Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1208910 | XP_013457733.1 | 95.146 | 206 | 10 | 0 | 1 | 206 | 1 | 206 | 9.18e-146 | 414 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1208910 | sp|Q9FGS5|NRT31_ARATH | 51.196 | 209 | 91 | 5 | 1 | 202 | 1 | 205 | 8.50e-64 | 199 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0004940 | Msa1208910 | 0.810834 | 9.464759e-51 | -8.615850e-47 |
| Msa0031650 | Msa1208910 | 0.811989 | 5.313560e-51 | -8.615850e-47 |
| Msa0059880 | Msa1208910 | 0.827104 | 1.880911e-54 | -8.615850e-47 |
| Msa0064990 | Msa1208910 | 0.805241 | 1.468307e-49 | -8.615850e-47 |
| Msa0093530 | Msa1208910 | 0.827792 | 1.286162e-54 | -8.615850e-47 |
| Msa0098380 | Msa1208910 | 0.810579 | 1.074737e-50 | -8.615850e-47 |
| Msa0107830 | Msa1208910 | 0.819057 | 1.417875e-52 | -8.615850e-47 |
| Msa0628200 | Msa1208910 | 0.808142 | 3.580630e-50 | -8.615850e-47 |
| Msa0629030 | Msa1208910 | 0.824294 | 8.724121e-54 | -8.615850e-47 |
| Msa0630130 | Msa1208910 | 0.846419 | 2.197439e-59 | -8.615850e-47 |
| Msa0634290 | Msa1208910 | 0.808444 | 3.088429e-50 | -8.615850e-47 |
| Msa0639400 | Msa1208910 | 0.814732 | 1.326429e-51 | -8.615850e-47 |
| Msa0639410 | Msa1208910 | 0.811780 | 5.899932e-51 | -8.615850e-47 |
| Msa0640290 | Msa1208910 | 0.801984 | 6.957999e-49 | -8.615850e-47 |
| Msa0645120 | Msa1208910 | 0.808679 | 2.750923e-50 | -8.615850e-47 |
| Msa0651820 | Msa1208910 | 0.801262 | 9.782563e-49 | -8.615850e-47 |
| Msa0657260 | Msa1208910 | 0.817922 | 2.564316e-52 | -8.615850e-47 |
| Msa0666580 | Msa1208910 | 0.821081 | 4.880296e-53 | -8.615850e-47 |
| Msa0668210 | Msa1208910 | 0.838467 | 2.827333e-57 | -8.615850e-47 |
| Msa0668220 | Msa1208910 | 0.809380 | 1.946696e-50 | -8.615850e-47 |
| Msa0668230 | Msa1208910 | 0.819905 | 9.084431e-53 | -8.615850e-47 |
| Msa0682420 | Msa1208910 | 0.813044 | 3.123484e-51 | -8.615850e-47 |
| Msa0701490 | Msa1208910 | 0.815720 | 7.998350e-52 | -8.615850e-47 |
| Msa0701560 | Msa1208910 | 0.816912 | 4.329754e-52 | -8.615850e-47 |
| Msa0715260 | Msa1208910 | 0.833143 | 6.316413e-56 | -8.615850e-47 |
| Msa0721260 | Msa1208910 | 0.847173 | 1.367466e-59 | -8.615850e-47 |
| Msa0728520 | Msa1208910 | 0.862107 | 6.404810e-64 | -8.615850e-47 |
| Msa0735340 | Msa1208910 | 0.801755 | 7.750514e-49 | -8.615850e-47 |
| Msa0738570 | Msa1208910 | 0.810393 | 1.178993e-50 | -8.615850e-47 |
| Msa0738660 | Msa1208910 | 0.810260 | 1.259243e-50 | -8.615850e-47 |
| Msa0753410 | Msa1208910 | 0.837525 | 4.938507e-57 | -8.615850e-47 |
| Msa0757240 | Msa1208910 | 0.834292 | 3.261722e-56 | -8.615850e-47 |
| Msa0776540 | Msa1208910 | 0.864579 | 1.098775e-64 | -8.615850e-47 |
| Msa0777070 | Msa1208910 | 0.803341 | 3.651243e-49 | -8.615850e-47 |
| Msa0783580 | Msa1208910 | 0.812097 | 5.032964e-51 | -8.615850e-47 |
| Msa0787860 | Msa1208910 | 0.800637 | 1.312825e-48 | -8.615850e-47 |
| Msa0793570 | Msa1208910 | 0.822526 | 2.259438e-53 | -8.615850e-47 |
| Msa0795320 | Msa1208910 | 0.805170 | 1.518844e-49 | -8.615850e-47 |
| Msa0795330 | Msa1208910 | 0.838930 | 2.145301e-57 | -8.615850e-47 |
| Msa0798230 | Msa1208910 | 0.838421 | 2.904915e-57 | -8.615850e-47 |
| Msa0819110 | Msa1208910 | 0.807710 | 4.425709e-50 | -8.615850e-47 |
| Msa0824860 | Msa1208910 | 0.801091 | 1.060559e-48 | -8.615850e-47 |
| Msa0826360 | Msa1208910 | 0.826273 | 2.970087e-54 | -8.615850e-47 |
| Msa0836740 | Msa1208910 | 0.831516 | 1.596814e-55 | -8.615850e-47 |
| Msa0837550 | Msa1208910 | 0.826911 | 2.091476e-54 | -8.615850e-47 |
| Msa0126960 | Msa1208910 | 0.826046 | 3.363573e-54 | -8.615850e-47 |
| Msa0139270 | Msa1208910 | 0.802555 | 5.307865e-49 | -8.615850e-47 |
| Msa0150910 | Msa1208910 | 0.818135 | 2.296004e-52 | -8.615850e-47 |
| Msa0171740 | Msa1208910 | 0.822603 | 2.168608e-53 | -8.615850e-47 |
| Msa0177550 | Msa1208910 | 0.805928 | 1.053240e-49 | -8.615850e-47 |
| Msa0194230 | Msa1208910 | 0.800808 | 1.211197e-48 | -8.615850e-47 |
| Msa0199860 | Msa1208910 | 0.832336 | 1.001700e-55 | -8.615850e-47 |
| Msa0200090 | Msa1208910 | 0.807758 | 4.321575e-50 | -8.615850e-47 |
| Msa0207930 | Msa1208910 | 0.838629 | 2.566504e-57 | -8.615850e-47 |
| Msa0207970 | Msa1208910 | 0.884613 | 1.650998e-71 | -8.615850e-47 |
| Msa0234120 | Msa1208910 | 0.826388 | 2.787460e-54 | -8.615850e-47 |
| Msa0390200 | Msa1208910 | 0.813531 | 2.441872e-51 | -8.615850e-47 |
| Msa0409490 | Msa1208910 | 0.800138 | 1.658924e-48 | -8.615850e-47 |
| Msa0414480 | Msa1208910 | 0.802302 | 5.983770e-49 | -8.615850e-47 |
| Msa0421080 | Msa1208910 | 0.822549 | 2.232312e-53 | -8.615850e-47 |
| Msa0464300 | Msa1208910 | 0.803792 | 2.943381e-49 | -8.615850e-47 |
| Msa0464310 | Msa1208910 | 0.801067 | 1.072345e-48 | -8.615850e-47 |
| Msa0985250 | Msa1208910 | 0.801570 | 8.461600e-49 | -8.615850e-47 |
| Msa0996980 | Msa1208910 | 0.810251 | 1.265183e-50 | -8.615850e-47 |
| Msa1003240 | Msa1208910 | 0.817933 | 2.549976e-52 | -8.615850e-47 |
| Msa1023430 | Msa1208910 | 0.841211 | 5.451746e-58 | -8.615850e-47 |
| Msa1024350 | Msa1208910 | 0.815742 | 7.910612e-52 | -8.615850e-47 |
| Msa1035050 | Msa1208910 | 0.804430 | 2.168563e-49 | -8.615850e-47 |
| Msa1037360 | Msa1208910 | 0.811219 | 7.810053e-51 | -8.615850e-47 |
| Msa1046220 | Msa1208910 | 0.818365 | 2.035989e-52 | -8.615850e-47 |
| Msa1048390 | Msa1208910 | 0.801556 | 8.515381e-49 | -8.615850e-47 |
| Msa1061670 | Msa1208910 | 0.801346 | 9.401172e-49 | -8.615850e-47 |
| Msa1061840 | Msa1208910 | 0.833253 | 5.930892e-56 | -8.615850e-47 |
| Msa1064940 | Msa1208910 | 0.829336 | 5.448286e-55 | -8.615850e-47 |
| Msa1087380 | Msa1208910 | 0.819043 | 1.428536e-52 | -8.615850e-47 |
| Msa0242390 | Msa1208910 | 0.827057 | 1.929975e-54 | -8.615850e-47 |
| Msa0243970 | Msa1208910 | 0.806473 | 8.085305e-50 | -8.615850e-47 |
| Msa0266640 | Msa1208910 | 0.829195 | 5.896547e-55 | -8.615850e-47 |
| Msa0266650 | Msa1208910 | 0.838061 | 3.595980e-57 | -8.615850e-47 |
| Msa0274250 | Msa1208910 | 0.800791 | 1.220940e-48 | -8.615850e-47 |
| Msa0274260 | Msa1208910 | 0.822956 | 1.794360e-53 | -8.615850e-47 |
| Msa0278950 | Msa1208910 | 0.803447 | 3.470339e-49 | -8.615850e-47 |
| Msa0280930 | Msa1208910 | 0.804894 | 1.735046e-49 | -8.615850e-47 |
| Msa0316230 | Msa1208910 | 0.805605 | 1.231426e-49 | -8.615850e-47 |
| Msa0318370 | Msa1208910 | 0.803800 | 2.932710e-49 | -8.615850e-47 |
| Msa0320130 | Msa1208910 | 0.834457 | 2.964909e-56 | -8.615850e-47 |
| Msa0320140 | Msa1208910 | 0.826226 | 3.046851e-54 | -8.615850e-47 |
| Msa0346170 | Msa1208910 | 0.822373 | 2.451945e-53 | -8.615850e-47 |
| Msa1107510 | Msa1208910 | 0.833505 | 5.133106e-56 | -8.615850e-47 |
| Msa1109890 | Msa1208910 | 0.805551 | 1.264045e-49 | -8.615850e-47 |
| Msa1110040 | Msa1208910 | 0.821897 | 3.162227e-53 | -8.615850e-47 |
| Msa1138110 | Msa1208910 | 0.821685 | 3.539567e-53 | -8.615850e-47 |
| Msa1151070 | Msa1208910 | 0.821583 | 3.738883e-53 | -8.615850e-47 |
| Msa1153690 | Msa1208910 | 0.803718 | 3.050230e-49 | -8.615850e-47 |
| Msa1173290 | Msa1208910 | 0.829453 | 5.104024e-55 | -8.615850e-47 |
| Msa1177090 | Msa1208910 | 0.804329 | 2.276672e-49 | -8.615850e-47 |
| Msa1208830 | Msa1208910 | 0.966019 | 2.298046e-125 | -8.615850e-47 |
| Msa1208910 | Msa1208920 | 0.801842 | 7.439988e-49 | -8.615850e-47 |
| Msa1208910 | Msa1221170 | 0.810782 | 9.712834e-51 | -8.615850e-47 |
| Msa1208910 | Msa1221520 | 0.800565 | 1.358100e-48 | -8.615850e-47 |
| Msa1208910 | Msa1263370 | 0.806785 | 6.947532e-50 | -8.615850e-47 |
| Msa1208910 | Msa1265500 | 0.811099 | 8.291948e-51 | -8.615850e-47 |
| Msa1208910 | Msa1269200 | 0.802163 | 6.392110e-49 | -8.615850e-47 |
| Msa1208910 | Msa1303150 | 0.809630 | 1.720845e-50 | -8.615850e-47 |
| Msa1208910 | Msa1323150 | 0.829498 | 4.976812e-55 | -8.615850e-47 |
| Msa1208910 | Msa1334000 | 0.802250 | 6.132426e-49 | -8.615850e-47 |
| Msa1208910 | Msa1341030 | 0.808481 | 3.032081e-50 | -8.615850e-47 |
| Msa1208910 | Msa1349420 | 0.807453 | 5.018540e-50 | -8.615850e-47 |
| Msa1208910 | Msa1398110 | 0.853769 | 1.919749e-61 | -8.615850e-47 |
| Msa1208910 | Msa1411550 | 0.809481 | 1.852491e-50 | -8.615850e-47 |
| Msa1208910 | Msa1419210 | 0.904186 | 1.595540e-79 | -8.615850e-47 |
| Msa1208910 | Msa1459930 | 0.802161 | 6.397196e-49 | -8.615850e-47 |
| Msa0489370 | Msa1208910 | 0.826927 | 2.073020e-54 | -8.615850e-47 |
| Msa0493710 | Msa1208910 | 0.844261 | 8.436118e-59 | -8.615850e-47 |
| Msa0533510 | Msa1208910 | 0.833926 | 4.026813e-56 | -8.615850e-47 |
| Msa0533520 | Msa1208910 | 0.838572 | 2.654924e-57 | -8.615850e-47 |
| Msa0538440 | Msa1208910 | 0.819277 | 1.263942e-52 | -8.615850e-47 |
| Msa0538450 | Msa1208910 | 0.828607 | 8.181750e-55 | -8.615850e-47 |
| Msa0538520 | Msa1208910 | 0.817971 | 2.500591e-52 | -8.615850e-47 |
| Msa0538530 | Msa1208910 | 0.816809 | 4.565687e-52 | -8.615850e-47 |
| Msa0546760 | Msa1208910 | 0.806662 | 7.377146e-50 | -8.615850e-47 |
| Msa0554660 | Msa1208910 | 0.805930 | 1.052181e-49 | -8.615850e-47 |
| Msa0588040 | Msa1208910 | 0.808337 | 3.254875e-50 | -8.615850e-47 |
| Msa0589720 | Msa1208910 | 0.827944 | 1.182440e-54 | -8.615850e-47 |
| Msa0589730 | Msa1208910 | 0.828997 | 6.585709e-55 | -8.615850e-47 |
| Msa0589740 | Msa1208910 | 0.808558 | 2.920305e-50 | -8.615850e-47 |
| Msa0589750 | Msa1208910 | 0.812108 | 5.004146e-51 | -8.615850e-47 |
| Msa0591490 | Msa1208910 | 0.807623 | 4.618014e-50 | -8.615850e-47 |
| Msa0594320 | Msa1208910 | 0.819779 | 9.708364e-53 | -8.615850e-47 |
| Msa0594330 | Msa1208910 | 0.848662 | 5.313475e-60 | -8.615850e-47 |
| Msa0597820 | Msa1208910 | 0.821533 | 3.839158e-53 | -8.615850e-47 |
| Msa0854840 | Msa1208910 | 0.809638 | 1.713750e-50 | -8.615850e-47 |
| Msa0859230 | Msa1208910 | 0.809630 | 1.720941e-50 | -8.615850e-47 |
| Msa0865830 | Msa1208910 | 0.810530 | 1.101032e-50 | -8.615850e-47 |
| Msa0868870 | Msa1208910 | 0.838529 | 2.725019e-57 | -8.615850e-47 |
| Msa0874750 | Msa1208910 | 0.810447 | 1.147363e-50 | -8.615850e-47 |
| Msa0886370 | Msa1208910 | 0.804963 | 1.678541e-49 | -8.615850e-47 |
| Msa0896920 | Msa1208910 | 0.818862 | 1.570415e-52 | -8.615850e-47 |
| Msa0910590 | Msa1208910 | 0.812677 | 3.759690e-51 | -8.615850e-47 |
| Msa0913500 | Msa1208910 | 0.802269 | 6.077827e-49 | -8.615850e-47 |
| Msa0932900 | Msa1208910 | 0.814326 | 1.630649e-51 | -8.615850e-47 |
| Msa0932910 | Msa1208910 | 0.807993 | 3.852385e-50 | -8.615850e-47 |
| Msa0932930 | Msa1208910 | 0.821009 | 5.071279e-53 | -8.615850e-47 |
| Msa0933580 | Msa1208910 | 0.812317 | 4.504794e-51 | -8.615850e-47 |
| Msa0948430 | Msa1208910 | 0.813134 | 2.984736e-51 | -8.615850e-47 |
| Msa0951060 | Msa1208910 | 0.818800 | 1.622745e-52 | -8.615850e-47 |
| Msa0970790 | Msa1208910 | 0.813101 | 3.034655e-51 | -8.615850e-47 |
| Msa0976400 | Msa1208910 | 0.818212 | 2.204766e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1208910 | MtrunA17_Chr4g0058331 | 95.146 | 206 | 10 | 0 | 1 | 206 | 1 | 206 | 8.45e-150 | 414 |
| Msa1208910 | MtrunA17_Chr4g0058311 | 95.631 | 206 | 9 | 0 | 1 | 206 | 1 | 206 | 2.82e-141 | 392 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1208910 | AT5G50200.1 | 51.196 | 209 | 91 | 5 | 1 | 202 | 1 | 205 | 8.64e-65 | 199 |
| Msa1208910 | AT5G50200.3 | 51.196 | 209 | 91 | 5 | 1 | 202 | 1 | 205 | 8.64e-65 | 199 |
| Msa1208910 | AT5G50200.2 | 51.196 | 209 | 91 | 5 | 1 | 202 | 1 | 205 | 8.64e-65 | 199 |
| Msa1208910 | AT4G24715.1 | 49.275 | 207 | 97 | 5 | 1 | 202 | 23 | 226 | 7.23e-63 | 195 |
Find 52 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTAGCATAACTTCAGCTTTA+TGG | 0.105787 | 8_1:+67262794 | None:intergenic |
| CATTGTCTTCAGTGCTTTCT+CGG | 0.283646 | 8_1:-67262472 | Msa1208910:CDS |
| ATCTATGCAGTTATGTTATC+TGG | 0.287035 | 8_1:-67262888 | Msa1208910:intron |
| CTCAAATTCAATCACTGCTT+TGG | 0.355774 | 8_1:+67262399 | None:intergenic |
| CGCATTTGATTCCAATGATA+AGG | 0.357587 | 8_1:-67262592 | Msa1208910:CDS |
| TACTTACCTTGTCCATGCTT+AGG | 0.361592 | 8_1:+67263010 | None:intergenic |
| ACTTACCTTGTCCATGCTTA+GGG | 0.362581 | 8_1:+67263011 | None:intergenic |
| TTTGAGATCAATGCAATTAG+TGG | 0.365486 | 8_1:-67262522 | Msa1208910:CDS |
| TCTTACCCGTGTTCGTGGTT+TGG | 0.369045 | 8_1:+67262556 | None:intergenic |
| ATAAGCCACTTCCTTATCAT+TGG | 0.373409 | 8_1:+67262581 | None:intergenic |
| GAATCAACCACTCGAAGGTT+TGG | 0.410270 | 8_1:+67262655 | None:intergenic |
| TGTTTAGCTGAAATTTGTTA+TGG | 0.421382 | 8_1:-67263082 | Msa1208910:CDS |
| AATGATAAGGAAGTGGCTTA+TGG | 0.425454 | 8_1:-67262579 | Msa1208910:CDS |
| GCTTTCTCGGTTGTGTCCCT+AGG | 0.446758 | 8_1:-67262459 | Msa1208910:CDS |
| GTCCCTGGAGAGTTCATCCT+CGG | 0.457081 | 8_1:+67262734 | None:intergenic |
| TAAGAAGAGTCTGTTCCAGC+TGG | 0.469266 | 8_1:+67262822 | None:intergenic |
| GCTACTCTTACCCGTGTTCG+TGG | 0.490262 | 8_1:+67262551 | None:intergenic |
| AGCTGAAATTTGTTATGGAA+AGG | 0.496497 | 8_1:-67263077 | Msa1208910:CDS |
| CTTCTATATTGAAAAGAGGA+AGG | 0.500127 | 8_1:-67262430 | Msa1208910:CDS |
| AGACCGAGGATGAACTCTCC+AGG | 0.511838 | 8_1:-67262737 | Msa1208910:CDS |
| TGATTCCAATGATAAGGAAG+TGG | 0.518875 | 8_1:-67262586 | Msa1208910:CDS |
| TGTTCTTCTATATTGAAAAG+AGG | 0.526365 | 8_1:-67262434 | Msa1208910:CDS |
| GTTGGAAGCATTGTAGGGCA+TGG | 0.526614 | 8_1:+67262686 | None:intergenic |
| TAGGGGAAGCTGTGACCTCA+AGG | 0.526901 | 8_1:+67263029 | None:intergenic |
| CAATATAGAAGAACACGCCT+AGG | 0.537687 | 8_1:+67262442 | None:intergenic |
| CTACAATGCTTCCAACAAAA+CGG | 0.547064 | 8_1:-67262679 | Msa1208910:CDS |
| TAGACAAAATCAGTGGAACA+TGG | 0.559795 | 8_1:-67262863 | Msa1208910:CDS |
| TTCTATATTGAAAAGAGGAA+GGG | 0.562873 | 8_1:-67262429 | Msa1208910:CDS |
| CGAGAAAGCACTGAAGACAA+TGG | 0.583492 | 8_1:+67262473 | None:intergenic |
| GTCACAGCTTCCCCTAAGCA+TGG | 0.584742 | 8_1:-67263022 | Msa1208910:CDS |
| CTAAACAAGACTTTCCCAGC+TGG | 0.586305 | 8_1:-67262837 | Msa1208910:CDS |
| TATGGCCAAACCACGAACAC+GGG | 0.596066 | 8_1:-67262561 | Msa1208910:CDS |
| TTATGGCCAAACCACGAACA+CGG | 0.597854 | 8_1:-67262562 | Msa1208910:CDS |
| CTTACCTTGTCCATGCTTAG+GGG | 0.615805 | 8_1:+67263012 | None:intergenic |
| AGACAAAATCAGTGGAACAT+GGG | 0.618150 | 8_1:-67262862 | Msa1208910:CDS |
| TATGGCAGCACACAAGCTTG+TGG | 0.620979 | 8_1:-67263125 | Msa1208910:CDS |
| TTCTTTGAAAAGAACCCTTG+AGG | 0.622622 | 8_1:-67263044 | Msa1208910:CDS |
| AAGAAGAGTCTGTTCCAGCT+GGG | 0.623941 | 8_1:+67262823 | None:intergenic |
| GACCGAGGATGAACTCTCCA+GGG | 0.630791 | 8_1:-67262736 | Msa1208910:CDS |
| AGGGGAAGCTGTGACCTCAA+GGG | 0.635053 | 8_1:+67263030 | None:intergenic |
| AATATAGAAGAACACGCCTA+GGG | 0.636139 | 8_1:+67262443 | None:intergenic |
| ATCATTGGAATCAAATGCGT+AGG | 0.647510 | 8_1:+67262596 | None:intergenic |
| CCTCGGTCTTTCTCCAAGCG+CGG | 0.651070 | 8_1:+67262751 | None:intergenic |
| GCGCAGAATCAACCACTCGA+AGG | 0.651463 | 8_1:+67262650 | None:intergenic |
| TCTGGAGTAGACAAAATCAG+TGG | 0.660740 | 8_1:-67262870 | Msa1208910:CDS |
| GCTTCCCCTAAGCATGGACA+AGG | 0.668529 | 8_1:-67263016 | Msa1208910:intron |
| AAACGGTCCAAACCTTCGAG+TGG | 0.693663 | 8_1:-67262662 | Msa1208910:CDS |
| AGCAGATATCAAGAGTCGCG+TGG | 0.697710 | 8_1:+67262496 | None:intergenic |
| AGATATCAAGAGTCGCGTGG+CGG | 0.700580 | 8_1:+67262499 | None:intergenic |
| CCGCGCTTGGAGAAAGACCG+AGG | 0.703551 | 8_1:-67262751 | Msa1208910:CDS |
| AAAGCTGAAGTTATGCTACG+CGG | 0.775950 | 8_1:-67262790 | Msa1208910:CDS |
| GTAGGCACGTACAAAGTACG+TGG | 0.784284 | 8_1:+67262614 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CAAAATTGAGTTTATATTGA+AGG | + | chr8_1:67262617-67262636 | None:intergenic | 20.0% |
| !!! | TGTTTAGCTGAAATTTGTTA+TGG | - | chr8_1:67262452-67262471 | Msa1208910:CDS | 25.0% |
| ! | TAATTAAATACGCCAATACA+AGG | + | chr8_1:67262566-67262585 | None:intergenic | 25.0% |
| ! | TGTTCTTCTATATTGAAAAG+AGG | - | chr8_1:67263100-67263119 | Msa1208910:CDS | 25.0% |
| ! | TTCTATATTGAAAAGAGGAA+GGG | - | chr8_1:67263105-67263124 | Msa1208910:CDS | 25.0% |
| !! | AGCTGAAATTTGTTATGGAA+AGG | - | chr8_1:67262457-67262476 | Msa1208910:CDS | 30.0% |
| ! | TAAGATTTTGCACCTTGTAT+TGG | - | chr8_1:67262551-67262570 | Msa1208910:CDS | 30.0% |
| ATCTATGCAGTTATGTTATC+TGG | - | chr8_1:67262646-67262665 | Msa1208910:CDS | 30.0% | |
| ! | TTTGAGATCAATGCAATTAG+TGG | - | chr8_1:67263012-67263031 | Msa1208910:intron | 30.0% |
| CTTCTATATTGAAAAGAGGA+AGG | - | chr8_1:67263104-67263123 | Msa1208910:CDS | 30.0% | |
| ! | TTCTTTGAAAAGAACCCTTG+AGG | - | chr8_1:67262490-67262509 | Msa1208910:CDS | 35.0% |
| TAGACAAAATCAGTGGAACA+TGG | - | chr8_1:67262671-67262690 | Msa1208910:CDS | 35.0% | |
| AGACAAAATCAGTGGAACAT+GGG | - | chr8_1:67262672-67262691 | Msa1208910:CDS | 35.0% | |
| GTAGCATAACTTCAGCTTTA+TGG | + | chr8_1:67262743-67262762 | None:intergenic | 35.0% | |
| !!! | GTTTTGTTGGAAGCATTGTA+GGG | + | chr8_1:67262856-67262875 | None:intergenic | 35.0% |
| CTACAATGCTTCCAACAAAA+CGG | - | chr8_1:67262855-67262874 | Msa1208910:CDS | 35.0% | |
| ATCATTGGAATCAAATGCGT+AGG | + | chr8_1:67262941-67262960 | None:intergenic | 35.0% | |
| CGCATTTGATTCCAATGATA+AGG | - | chr8_1:67262942-67262961 | Msa1208910:intron | 35.0% | |
| TGATTCCAATGATAAGGAAG+TGG | - | chr8_1:67262948-67262967 | Msa1208910:intron | 35.0% | |
| ATAAGCCACTTCCTTATCAT+TGG | + | chr8_1:67262956-67262975 | None:intergenic | 35.0% | |
| AATGATAAGGAAGTGGCTTA+TGG | - | chr8_1:67262955-67262974 | Msa1208910:intron | 35.0% | |
| AATATAGAAGAACACGCCTA+GGG | + | chr8_1:67263094-67263113 | None:intergenic | 35.0% | |
| ACTTACCTTGTCCATGCTTA+GGG | + | chr8_1:67262526-67262545 | None:intergenic | 40.0% | |
| TACTTACCTTGTCCATGCTT+AGG | + | chr8_1:67262527-67262546 | None:intergenic | 40.0% | |
| ! | TCTGGAGTAGACAAAATCAG+TGG | - | chr8_1:67262664-67262683 | Msa1208910:CDS | 40.0% |
| AAAGCTGAAGTTATGCTACG+CGG | - | chr8_1:67262744-67262763 | Msa1208910:CDS | 40.0% | |
| !!! | CGTTTTGTTGGAAGCATTGT+AGG | + | chr8_1:67262857-67262876 | None:intergenic | 40.0% |
| CATTGTCTTCAGTGCTTTCT+CGG | - | chr8_1:67263062-67263081 | Msa1208910:CDS | 40.0% | |
| CAATATAGAAGAACACGCCT+AGG | + | chr8_1:67263095-67263114 | None:intergenic | 40.0% | |
| CTTACCTTGTCCATGCTTAG+GGG | + | chr8_1:67262525-67262544 | None:intergenic | 45.0% | |
| CTAAACAAGACTTTCCCAGC+TGG | - | chr8_1:67262697-67262716 | Msa1208910:CDS | 45.0% | |
| AAGAAGAGTCTGTTCCAGCT+GGG | + | chr8_1:67262714-67262733 | None:intergenic | 45.0% | |
| TAAGAAGAGTCTGTTCCAGC+TGG | + | chr8_1:67262715-67262734 | None:intergenic | 45.0% | |
| !! | GTGTTGACAAGTTTTGTCCC+TGG | + | chr8_1:67262818-67262837 | None:intergenic | 45.0% |
| ! | GAAGGTTTGGACCGTTTTGT+TGG | + | chr8_1:67262869-67262888 | None:intergenic | 45.0% |
| GAATCAACCACTCGAAGGTT+TGG | + | chr8_1:67262882-67262901 | None:intergenic | 45.0% | |
| TTATGGCCAAACCACGAACA+CGG | - | chr8_1:67262972-67262991 | Msa1208910:intron | 45.0% | |
| !! | CGAGAAAGCACTGAAGACAA+TGG | + | chr8_1:67263064-67263083 | None:intergenic | 45.0% |
| !!! | AGCGCGGTCTTTTTGGCTTA+TGG | + | chr8_1:67262770-67262789 | None:intergenic | 50.0% |
| TAAGCCAAAAAGACCGCGCT+TGG | - | chr8_1:67262770-67262789 | Msa1208910:CDS | 50.0% | |
| ! | TTCTCCAAGCGCGGTCTTTT+TGG | + | chr8_1:67262777-67262796 | None:intergenic | 50.0% |
| !! | GTTGGAAGCATTGTAGGGCA+TGG | + | chr8_1:67262851-67262870 | None:intergenic | 50.0% |
| AAACGGTCCAAACCTTCGAG+TGG | - | chr8_1:67262872-67262891 | Msa1208910:CDS | 50.0% | |
| GTAGGCACGTACAAAGTACG+TGG | + | chr8_1:67262923-67262942 | None:intergenic | 50.0% | |
| TATGGCCAAACCACGAACAC+GGG | - | chr8_1:67262973-67262992 | Msa1208910:intron | 50.0% | |
| TCTTACCCGTGTTCGTGGTT+TGG | + | chr8_1:67262981-67263000 | None:intergenic | 50.0% | |
| AGATATCAAGAGTCGCGTGG+CGG | + | chr8_1:67263038-67263057 | None:intergenic | 50.0% | |
| AGCAGATATCAAGAGTCGCG+TGG | + | chr8_1:67263041-67263060 | None:intergenic | 50.0% | |
| AGGGGAAGCTGTGACCTCAA+GGG | + | chr8_1:67262507-67262526 | None:intergenic | 55.0% | |
| TAGGGGAAGCTGTGACCTCA+AGG | + | chr8_1:67262508-67262527 | None:intergenic | 55.0% | |
| GTCACAGCTTCCCCTAAGCA+TGG | - | chr8_1:67262512-67262531 | Msa1208910:CDS | 55.0% | |
| GCTTCCCCTAAGCATGGACA+AGG | - | chr8_1:67262518-67262537 | Msa1208910:CDS | 55.0% | |
| AGACCGAGGATGAACTCTCC+AGG | - | chr8_1:67262797-67262816 | Msa1208910:CDS | 55.0% | |
| GACCGAGGATGAACTCTCCA+GGG | - | chr8_1:67262798-67262817 | Msa1208910:CDS | 55.0% | |
| GTCCCTGGAGAGTTCATCCT+CGG | + | chr8_1:67262803-67262822 | None:intergenic | 55.0% | |
| GCGCAGAATCAACCACTCGA+AGG | + | chr8_1:67262887-67262906 | None:intergenic | 55.0% | |
| CGTACAAAGTACGTGGCCTG+AGG | + | chr8_1:67262916-67262935 | None:intergenic | 55.0% | |
| GCTACTCTTACCCGTGTTCG+TGG | + | chr8_1:67262986-67263005 | None:intergenic | 55.0% | |
| GCTTTCTCGGTTGTGTCCCT+AGG | - | chr8_1:67263075-67263094 | Msa1208910:CDS | 55.0% | |
| CCTCGGTCTTTCTCCAAGCG+CGG | + | chr8_1:67262786-67262805 | None:intergenic | 60.0% | |
| CCGCGCTTGGAGAAAGACCG+AGG | - | chr8_1:67262783-67262802 | Msa1208910:CDS | 65.0% | |
| GATTCTGCGCGACGTGCCTC+AGG | - | chr8_1:67262897-67262916 | Msa1208910:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 67262410 | 67263146 | 67262410 | ID=Msa1208910;Name=Msa1208910 |
| chr8_1 | mRNA | 67262410 | 67263146 | 67262410 | ID=Msa1208910-mRNA-1;Parent=Msa1208910;Name=Msa1208910-mRNA-1;_AED=0.31;_eAED=0.31;_QI=0|1|0|1|1|1|2|0|206 |
| chr8_1 | exon | 67263017 | 67263146 | 67263017 | ID=Msa1208910-mRNA-1:exon:22162;Parent=Msa1208910-mRNA-1 |
| chr8_1 | exon | 67262410 | 67262900 | 67262410 | ID=Msa1208910-mRNA-1:exon:22161;Parent=Msa1208910-mRNA-1 |
| chr8_1 | CDS | 67263017 | 67263146 | 67263017 | ID=Msa1208910-mRNA-1:cds;Parent=Msa1208910-mRNA-1 |
| chr8_1 | CDS | 67262410 | 67262900 | 67262410 | ID=Msa1208910-mRNA-1:cds;Parent=Msa1208910-mRNA-1 |
| Gene Sequence |
| Protein sequence |
