Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0017510 | Msa1069100 | 0.803755 | 2.996204e-49 | -8.615850e-47 |
| Msa0036430 | Msa1069100 | 0.803765 | 2.981264e-49 | -8.615850e-47 |
| Msa0038350 | Msa1069100 | -0.802562 | 5.289637e-49 | -8.615850e-47 |
| Msa0101810 | Msa1069100 | -0.829312 | 5.524548e-55 | -8.615850e-47 |
| Msa0604360 | Msa1069100 | 0.820783 | 5.714440e-53 | -8.615850e-47 |
| Msa0631120 | Msa1069100 | 0.823993 | 1.026839e-53 | -8.615850e-47 |
| Msa0647130 | Msa1069100 | 0.809208 | 2.120214e-50 | -8.615850e-47 |
| Msa0677400 | Msa1069100 | 0.825668 | 4.134540e-54 | -8.615850e-47 |
| Msa0691160 | Msa1069100 | 0.809217 | 2.110567e-50 | -8.615850e-47 |
| Msa0716450 | Msa1069100 | 0.827011 | 1.980053e-54 | -8.615850e-47 |
| Msa0719490 | Msa1069100 | -0.801277 | 9.713279e-49 | -8.615850e-47 |
| Msa0741900 | Msa1069100 | 0.835235 | 1.888485e-56 | -8.615850e-47 |
| Msa0754720 | Msa1069100 | -0.838598 | 2.614243e-57 | -8.615850e-47 |
| Msa0756630 | Msa1069100 | -0.839693 | 1.359845e-57 | -8.615850e-47 |
| Msa0789570 | Msa1069100 | 0.814774 | 1.298240e-51 | -8.615850e-47 |
| Msa0799030 | Msa1069100 | 0.800747 | 1.246798e-48 | -8.615850e-47 |
| Msa0802470 | Msa1069100 | -0.810538 | 1.096743e-50 | -8.615850e-47 |
| Msa0804680 | Msa1069100 | 0.804246 | 2.368874e-49 | -8.615850e-47 |
| Msa0810020 | Msa1069100 | 0.815268 | 1.008646e-51 | -8.615850e-47 |
| Msa0822990 | Msa1069100 | 0.807156 | 5.800145e-50 | -8.615850e-47 |
| Msa0829620 | Msa1069100 | 0.807071 | 6.045369e-50 | -8.615850e-47 |
| Msa0837440 | Msa1069100 | 0.803377 | 3.589523e-49 | -8.615850e-47 |
| Msa0130570 | Msa1069100 | 0.837238 | 5.846894e-57 | -8.615850e-47 |
| Msa0157290 | Msa1069100 | 0.803887 | 2.812506e-49 | -8.615850e-47 |
| Msa0168100 | Msa1069100 | 0.800203 | 1.609129e-48 | -8.615850e-47 |
| Msa0172350 | Msa1069100 | 0.811394 | 7.156480e-51 | -8.615850e-47 |
| Msa0188680 | Msa1069100 | -0.801510 | 8.704398e-49 | -8.615850e-47 |
| Msa0191040 | Msa1069100 | 0.808097 | 3.660409e-50 | -8.615850e-47 |
| Msa0191250 | Msa1069100 | 0.809447 | 1.883719e-50 | -8.615850e-47 |
| Msa0203030 | Msa1069100 | 0.806204 | 9.215224e-50 | -8.615850e-47 |
| Msa0203640 | Msa1069100 | 0.800319 | 1.524092e-48 | -8.615850e-47 |
| Msa0233340 | Msa1069100 | 0.807960 | 3.915347e-50 | -8.615850e-47 |
| Msa0369570 | Msa1069100 | 0.806741 | 7.099803e-50 | -8.615850e-47 |
| Msa0407910 | Msa1069100 | 0.804332 | 2.272382e-49 | -8.615850e-47 |
| Msa0407920 | Msa1069100 | 0.803640 | 3.165420e-49 | -8.615850e-47 |
| Msa0414170 | Msa1069100 | 0.813365 | 2.656416e-51 | -8.615850e-47 |
| Msa0423360 | Msa1069100 | -0.842533 | 2.439815e-58 | -8.615850e-47 |
| Msa0436280 | Msa1069100 | -0.805222 | 1.481243e-49 | -8.615850e-47 |
| Msa0437710 | Msa1069100 | 0.839785 | 1.287198e-57 | -8.615850e-47 |
| Msa0448550 | Msa1069100 | -0.801692 | 7.988116e-49 | -8.615850e-47 |
| Msa0981010 | Msa1069100 | 0.815393 | 9.461424e-52 | -8.615850e-47 |
| Msa0990960 | Msa1069100 | 0.810589 | 1.069194e-50 | -8.615850e-47 |
| Msa0996080 | Msa1069100 | 0.801146 | 1.033264e-48 | -8.615850e-47 |
| Msa1007270 | Msa1069100 | 0.807481 | 4.948436e-50 | -8.615850e-47 |
| Msa1008490 | Msa1069100 | 0.826665 | 2.394247e-54 | -8.615850e-47 |
| Msa1019020 | Msa1069100 | 0.816289 | 5.970575e-52 | -8.615850e-47 |
| Msa1037700 | Msa1069100 | -0.804240 | 2.375070e-49 | -8.615850e-47 |
| Msa1040120 | Msa1069100 | 0.827618 | 1.416598e-54 | -8.615850e-47 |
| Msa1069100 | Msa1083410 | 0.809458 | 1.873102e-50 | -8.615850e-47 |
| Msa1069100 | Msa1085300 | 0.803028 | 4.237525e-49 | -8.615850e-47 |
| Msa1069100 | Msa1097640 | 0.822399 | 2.419108e-53 | -8.615850e-47 |
| Msa1069100 | Msa1098560 | 0.836120 | 1.127029e-56 | -8.615850e-47 |
| Msa1069100 | Msa1124680 | -0.812292 | 4.563221e-51 | -8.615850e-47 |
| Msa1069100 | Msa1139810 | 0.811290 | 7.539396e-51 | -8.615850e-47 |
| Msa1069100 | Msa1147570 | 0.808803 | 2.587660e-50 | -8.615850e-47 |
| Msa1069100 | Msa1156970 | 0.800650 | 1.304884e-48 | -8.615850e-47 |
| Msa1069100 | Msa1217860 | 0.803362 | 3.614473e-49 | -8.615850e-47 |
| Msa1069100 | Msa1217900 | -0.823522 | 1.323975e-53 | -8.615850e-47 |
| Msa1069100 | Msa1242230 | 0.807195 | 5.689828e-50 | -8.615850e-47 |
| Msa1069100 | Msa1266960 | 0.805513 | 1.287461e-49 | -8.615850e-47 |
| Msa1069100 | Msa1269580 | -0.807163 | 5.780294e-50 | -8.615850e-47 |
| Msa1069100 | Msa1271280 | 0.803293 | 3.736473e-49 | -8.615850e-47 |
| Msa1069100 | Msa1271300 | 0.818784 | 1.636109e-52 | -8.615850e-47 |
| Msa1069100 | Msa1284260 | 0.808637 | 2.808541e-50 | -8.615850e-47 |
| Msa1069100 | Msa1322860 | 0.821131 | 4.752402e-53 | -8.615850e-47 |
| Msa1069100 | Msa1323190 | 0.814819 | 1.268440e-51 | -8.615850e-47 |
| Msa1069100 | Msa1323670 | 0.808572 | 2.899783e-50 | -8.615850e-47 |
| Msa1069100 | Msa1327050 | 0.819910 | 9.061635e-53 | -8.615850e-47 |
| Msa1069100 | Msa1329920 | 0.800484 | 1.410382e-48 | -8.615850e-47 |
| Msa1069100 | Msa1331910 | 0.811319 | 7.431139e-51 | -8.615850e-47 |
| Msa1069100 | Msa1390310 | 0.810756 | 9.838498e-51 | -8.615850e-47 |
| Msa1069100 | Msa1399560 | 0.812895 | 3.367763e-51 | -8.615850e-47 |
| Msa1069100 | Msa1405180 | 0.805852 | 1.092596e-49 | -8.615850e-47 |
| Msa1069100 | Msa1429250 | 0.801003 | 1.104995e-48 | -8.615850e-47 |
| Msa1069100 | Msa1429360 | -0.805673 | 1.191565e-49 | -8.615850e-47 |
| Msa1069100 | Msa1429420 | 0.809975 | 1.450858e-50 | -8.615850e-47 |
| Msa1069100 | Msa1443290 | -0.803258 | 3.798097e-49 | -8.615850e-47 |
| Msa1069100 | Msa1443840 | 0.801029 | 1.091917e-48 | -8.615850e-47 |
| Msa1069100 | Msa1460020 | 0.800693 | 1.278852e-48 | -8.615850e-47 |
| Msa0260820 | Msa1069100 | -0.800694 | 1.278072e-48 | -8.615850e-47 |
| Msa0264350 | Msa1069100 | 0.814940 | 1.192407e-51 | -8.615850e-47 |
| Msa0267760 | Msa1069100 | 0.814674 | 1.365985e-51 | -8.615850e-47 |
| Msa0272480 | Msa1069100 | -0.802271 | 6.072621e-49 | -8.615850e-47 |
| Msa0306300 | Msa1069100 | 0.806997 | 6.267460e-50 | -8.615850e-47 |
| Msa0307830 | Msa1069100 | -0.805431 | 1.339082e-49 | -8.615850e-47 |
| Msa0311690 | Msa1069100 | 0.801417 | 9.091492e-49 | -8.615850e-47 |
| Msa0321630 | Msa1069100 | -0.803026 | 4.242998e-49 | -8.615850e-47 |
| Msa0356890 | Msa1069100 | -0.804666 | 1.935781e-49 | -8.615850e-47 |
| Msa0509610 | Msa1069100 | 0.814060 | 1.867516e-51 | -8.615850e-47 |
| Msa0509970 | Msa1069100 | 0.841116 | 5.773995e-58 | -8.615850e-47 |
| Msa0526710 | Msa1069100 | 0.808499 | 3.006349e-50 | -8.615850e-47 |
| Msa0534590 | Msa1069100 | 0.810378 | 1.187622e-50 | -8.615850e-47 |
| Msa0540870 | Msa1069100 | 0.820738 | 5.851353e-53 | -8.615850e-47 |
| Msa0557680 | Msa1069100 | 0.806460 | 8.138576e-50 | -8.615850e-47 |
| Msa0566500 | Msa1069100 | -0.809709 | 1.654648e-50 | -8.615850e-47 |
| Msa0590800 | Msa1069100 | 0.813775 | 2.157340e-51 | -8.615850e-47 |
| Msa0858840 | Msa1069100 | 0.806112 | 9.635469e-50 | -8.615850e-47 |
| Msa0862100 | Msa1069100 | 0.810343 | 1.208316e-50 | -8.615850e-47 |
| Msa0867890 | Msa1069100 | 0.808162 | 3.545890e-50 | -8.615850e-47 |
| Msa0870530 | Msa1069100 | 0.806612 | 7.557326e-50 | -8.615850e-47 |
| Msa0880140 | Msa1069100 | 0.804485 | 2.112068e-49 | -8.615850e-47 |
| Msa0891040 | Msa1069100 | 0.801227 | 9.944977e-49 | -8.615850e-47 |
| Msa0896220 | Msa1069100 | 0.800172 | 1.632607e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 12 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGTTCTCAATAATGAAGTTT+TGG | 0.330937 | 7_2:-64643013 | Msa1069100:CDS |
| CACAGCTACTTGGCGACCAT+CGG | 0.442776 | 7_2:+64642967 | None:intergenic |
| AACTCCTTCCTCAACTCTCC+TGG | 0.449479 | 7_2:+64643065 | None:intergenic |
| TTCCTCAACTCTCCTGGAGA+TGG | 0.454694 | 7_2:+64643071 | None:intergenic |
| ACAGCTACTTGGCGACCATC+GGG | 0.494763 | 7_2:+64642968 | None:intergenic |
| TTACTATGGGTGGGCGAAAG+AGG | 0.510118 | 7_2:-64643113 | None:intergenic |
| TGAATGTGAGGGCCATCTCC+AGG | 0.525033 | 7_2:-64643083 | Msa1069100:CDS |
| CAATAAACCATTACTATGGG+TGG | 0.534098 | 7_2:-64643123 | None:intergenic |
| GAGAACTGCTTTCCGTACCT+TGG | 0.538578 | 7_2:+64643029 | None:intergenic |
| GGCCATCTCCAGGAGAGTTG+AGG | 0.573131 | 7_2:-64643073 | Msa1069100:CDS |
| AATAAACCATTACTATGGGT+GGG | 0.624893 | 7_2:-64643122 | None:intergenic |
| TCACAAGAATGACCAAGGTA+CGG | 0.626131 | 7_2:-64643041 | Msa1069100:CDS |
| AGAGGAGTGAAATGAATGTG+AGG | 0.631352 | 7_2:-64643095 | Msa1069100:CDS |
| ATCTCCAGGAGAGTTGAGGA+AGG | 0.641416 | 7_2:-64643069 | Msa1069100:CDS |
| GAGGAGTGAAATGAATGTGA+GGG | 0.646561 | 7_2:-64643094 | Msa1069100:CDS |
| TACAAATCAAGCTTACCCGA+TGG | 0.661383 | 7_2:-64642983 | Msa1069100:CDS |
| AGTTCTCACAAGAATGACCA+AGG | 0.691365 | 7_2:-64643046 | Msa1069100:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGTTCTCAATAATGAAGTTT+TGG | - | chr7_2:64643067-64643086 | Msa1069100:CDS | 25.0% |
| AGAGGAGTGAAATGAATGTG+AGG | - | chr7_2:64642985-64643004 | Msa1069100:CDS | 40.0% | |
| GAGGAGTGAAATGAATGTGA+GGG | - | chr7_2:64642986-64643005 | Msa1069100:CDS | 40.0% | |
| AGTTCTCACAAGAATGACCA+AGG | - | chr7_2:64643034-64643053 | Msa1069100:CDS | 40.0% | |
| TCACAAGAATGACCAAGGTA+CGG | - | chr7_2:64643039-64643058 | Msa1069100:CDS | 40.0% | |
| TACAAATCAAGCTTACCCGA+TGG | - | chr7_2:64643097-64643116 | Msa1069100:CDS | 40.0% | |
| TTCCTCAACTCTCCTGGAGA+TGG | + | chr7_2:64643012-64643031 | None:intergenic | 50.0% | |
| ATCTCCAGGAGAGTTGAGGA+AGG | - | chr7_2:64643011-64643030 | Msa1069100:CDS | 50.0% | |
| AACTCCTTCCTCAACTCTCC+TGG | + | chr7_2:64643018-64643037 | None:intergenic | 50.0% | |
| GAGAACTGCTTTCCGTACCT+TGG | + | chr7_2:64643054-64643073 | None:intergenic | 50.0% | |
| TGAATGTGAGGGCCATCTCC+AGG | - | chr7_2:64642997-64643016 | Msa1069100:CDS | 55.0% | |
| GGCCATCTCCAGGAGAGTTG+AGG | - | chr7_2:64643007-64643026 | Msa1069100:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7_2 | gene | 64642972 | 64643130 | 64642972 | ID=Msa1069100;Name=Msa1069100 |
| chr7_2 | mRNA | 64642972 | 64643130 | 64642972 | ID=Msa1069100-mRNA-1;Parent=Msa1069100;Name=Msa1069100-mRNA-1;_AED=0.84;_eAED=1.00;_QI=0|-1|0|1|-1|1|1|0|52 |
| chr7_2 | exon | 64642972 | 64643130 | 64642972 | ID=Msa1069100-mRNA-1:exon:13495;Parent=Msa1069100-mRNA-1 |
| chr7_2 | CDS | 64642972 | 64643130 | 64642972 | ID=Msa1069100-mRNA-1:cds;Parent=Msa1069100-mRNA-1 |
| Gene Sequence |
| Protein sequence |
