AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1358960


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1358960 MtrunA17_Chr2g0333351 98.571 210 2 1 1 210 1 209 9.61e-156 429
Msa1358960 MtrunA17_Chr4g0003001 60.181 221 64 10 1 210 58 265 1.02e-80 241
Msa1358960 MtrunA17_Chr4g0064971 45.912 159 70 3 1 158 1 144 2.10e-34 122
Msa1358960 MtrunA17_Chr2g0304551 38.378 185 86 6 1 165 1 177 1.63e-29 109
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1358960 AT1G15760.1 44.444 225 79 8 3 209 5 201 1.03e-57 181
Msa1358960 AT1G80520.1 47.489 219 77 8 1 209 1 191 9.86e-51 163
Msa1358960 AT2G12462.1 29.231 260 121 7 1 209 1 248 4.88e-26 101

Find 42 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTAGGCCTAATGGTGGATT+TGG 0.285518 tig0016604:-15933 Msa1358960:CDS
GCTTTGTCTGGTGATCTTGA+TGG 0.316091 tig0016604:-15795 Msa1358960:CDS
GAGAATTTCTAATCTGTGTT+TGG 0.325351 tig0016604:+16097 None:intergenic
TAAGTGGGATACCATGTTTA+AGG 0.337355 tig0016604:-15652 Msa1358960:CDS
GAGAAAATGTTTCTCACAAA+TGG 0.339341 tig0016604:-15852 Msa1358960:CDS
CCATCTCTTGTATATGAGTA+TGG 0.354378 tig0016604:-16206 Msa1358960:CDS
TACTGAAGAATCAGCACTTA+TGG 0.357191 tig0016604:-15964 Msa1358960:CDS
AGTACTTAGCTAACTATGTT+AGG 0.383843 tig0016604:-15998 Msa1358960:CDS
TTCTAATCTGTGTTTGGCTA+TGG 0.404684 tig0016604:+16103 None:intergenic
ATAATCATGATGATGGTTAT+TGG 0.406535 tig0016604:-15695 Msa1358960:CDS
CTAGGCCTAATGGTGGATTT+GGG 0.437057 tig0016604:-15932 Msa1358960:CDS
ACAGTAGCTCCTGCTTTGTC+TGG 0.454059 tig0016604:-15807 Msa1358960:CDS
CACTTATGGTGATGCCAACT+AGG 0.468970 tig0016604:-15950 Msa1358960:CDS
GAAGAGGAACACACAATTTG+TGG 0.477404 tig0016604:-15886 Msa1358960:CDS
CCATGCTTTGTAGAAACTCA+TGG 0.497469 tig0016604:+16132 None:intergenic
TTAACTCAACAGCCATGGAT+TGG 0.500177 tig0016604:-16259 None:intergenic
TTTCCAAAAGGAGCAAAAGA+TGG 0.506781 tig0016604:-15748 Msa1358960:CDS
AATCCACCATTAGGCCTAGT+TGG 0.510240 tig0016604:+15936 None:intergenic
ATGGTGGATTTGGGAGAAGA+TGG 0.518326 tig0016604:-15923 Msa1358960:CDS
CCATGAGTTTCTACAAAGCA+TGG 0.527981 tig0016604:-16132 Msa1358960:CDS
CAAAAGATGGATGGAGATGA+TGG 0.529408 tig0016604:-15735 Msa1358960:CDS
TACAAGAGATGGCTCAAGGT+TGG 0.536104 tig0016604:+16217 None:intergenic
CTGCTGTTGAAGAGATTAAG+TGG 0.538740 tig0016604:-15668 Msa1358960:CDS
CTTCTCCCAAATCCACCATT+AGG 0.539738 tig0016604:+15927 None:intergenic
GTGATGCCAACTAGGCCTAA+TGG 0.547391 tig0016604:-15942 Msa1358960:CDS
GATGGAGATGATGGTGGTGA+TGG 0.550508 tig0016604:-15726 Msa1358960:CDS
TGTTGTGTATCATTTCCAAA+AGG 0.557274 tig0016604:-15760 Msa1358960:CDS
CAAAAGGAGCAAAAGATGGA+TGG 0.561894 tig0016604:-15744 Msa1358960:CDS
AATAATGATAATCATGATGA+TGG 0.562055 tig0016604:-15702 Msa1358960:CDS
CCATACTCATATACAAGAGA+TGG 0.573633 tig0016604:+16206 None:intergenic
TCAAGATCACCAGACAAAGC+AGG 0.592387 tig0016604:+15798 None:intergenic
CATGAGTTTCTACAAAGCAT+GGG 0.601787 tig0016604:-16131 Msa1358960:CDS
GGAAGAGTGCTATAATGAAG+AGG 0.605141 tig0016604:-15902 Msa1358960:CDS
AAATGTTTCTCACAAATGGA+AGG 0.609404 tig0016604:-15848 Msa1358960:CDS
CATATACAAGAGATGGCTCA+AGG 0.614307 tig0016604:+16213 None:intergenic
AATTTGTAGCTGCAATCAAG+AGG 0.614506 tig0016604:-16028 Msa1358960:CDS
TAGCCATGAAAACCAATCCA+TGG 0.623251 tig0016604:+16247 None:intergenic
TGCTGTTGAAGAGATTAAGT+GGG 0.633418 tig0016604:-15667 Msa1358960:CDS
TGGCTTTAGATCCTTAAACA+TGG 0.634954 tig0016604:+15641 None:intergenic
ATGCCAACTAGGCCTAATGG+TGG 0.656564 tig0016604:-15939 Msa1358960:CDS
GAGGAACACACAATTTGTGG+TGG 0.682058 tig0016604:-15883 Msa1358960:CDS
AAGATGGATGGAGATGATGG+TGG 0.707845 tig0016604:-15732 Msa1358960:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAATGATAATCATGATGA+TGG - tig0016604:16178-16197 Msa1358960:CDS 20.0%
!!! ATAATCATGATGATGGTTAT+TGG - tig0016604:16185-16204 Msa1358960:CDS 25.0%
GAGAATTTCTAATCTGTGTT+TGG + tig0016604:15786-15805 None:intergenic 30.0%
AGTACTTAGCTAACTATGTT+AGG - tig0016604:15882-15901 Msa1358960:CDS 30.0%
GAGAAAATGTTTCTCACAAA+TGG - tig0016604:16028-16047 Msa1358960:CDS 30.0%
AAATGTTTCTCACAAATGGA+AGG - tig0016604:16032-16051 Msa1358960:CDS 30.0%
!! TTTTGGAAATGATACACAAC+AGG + tig0016604:16121-16140 None:intergenic 30.0%
TGTTGTGTATCATTTCCAAA+AGG - tig0016604:16120-16139 Msa1358960:CDS 30.0%
CCATACTCATATACAAGAGA+TGG + tig0016604:15677-15696 None:intergenic 35.0%
CCATCTCTTGTATATGAGTA+TGG - tig0016604:15674-15693 Msa1358960:CDS 35.0%
CATGAGTTTCTACAAAGCAT+GGG - tig0016604:15749-15768 Msa1358960:CDS 35.0%
TTCTAATCTGTGTTTGGCTA+TGG + tig0016604:15780-15799 None:intergenic 35.0%
! ACAAATTTTGCAACTGTGTG+TGG + tig0016604:15839-15858 None:intergenic 35.0%
AATTTGTAGCTGCAATCAAG+AGG - tig0016604:15852-15871 Msa1358960:CDS 35.0%
TACTGAAGAATCAGCACTTA+TGG - tig0016604:15916-15935 Msa1358960:CDS 35.0%
TTTCCAAAAGGAGCAAAAGA+TGG - tig0016604:16132-16151 Msa1358960:CDS 35.0%
TGCTGTTGAAGAGATTAAGT+GGG - tig0016604:16213-16232 Msa1358960:CDS 35.0%
TAAGTGGGATACCATGTTTA+AGG - tig0016604:16228-16247 Msa1358960:CDS 35.0%
!! TGGCTTTAGATCCTTAAACA+TGG + tig0016604:16242-16261 None:intergenic 35.0%
CATATACAAGAGATGGCTCA+AGG + tig0016604:15670-15689 None:intergenic 40.0%
! GCTCATTTTGTGCAAAGACA+AGG + tig0016604:15700-15719 None:intergenic 40.0%
CCATGCTTTGTAGAAACTCA+TGG + tig0016604:15751-15770 None:intergenic 40.0%
CCATGAGTTTCTACAAAGCA+TGG - tig0016604:15748-15767 Msa1358960:CDS 40.0%
!! GGAAGAGTGCTATAATGAAG+AGG - tig0016604:15978-15997 Msa1358960:CDS 40.0%
GAAGAGGAACACACAATTTG+TGG - tig0016604:15994-16013 Msa1358960:CDS 40.0%
! AAAGCAGGAGCTACTGTTTT+TGG + tig0016604:16070-16089 None:intergenic 40.0%
! CATCCATCTTTTGCTCCTTT+TGG + tig0016604:16138-16157 None:intergenic 40.0%
CAAAAGGAGCAAAAGATGGA+TGG - tig0016604:16136-16155 Msa1358960:CDS 40.0%
CAAAAGATGGATGGAGATGA+TGG - tig0016604:16145-16164 Msa1358960:CDS 40.0%
CTGCTGTTGAAGAGATTAAG+TGG - tig0016604:16212-16231 Msa1358960:CDS 40.0%
TACAAGAGATGGCTCAAGGT+TGG + tig0016604:15666-15685 None:intergenic 45.0%
! CACTTATGGTGATGCCAACT+AGG - tig0016604:15930-15949 Msa1358960:CDS 45.0%
AATCCACCATTAGGCCTAGT+TGG + tig0016604:15947-15966 None:intergenic 45.0%
!! ACTAGGCCTAATGGTGGATT+TGG - tig0016604:15947-15966 Msa1358960:CDS 45.0%
!! CTAGGCCTAATGGTGGATTT+GGG - tig0016604:15948-15967 Msa1358960:CDS 45.0%
CTTCTCCCAAATCCACCATT+AGG + tig0016604:15956-15975 None:intergenic 45.0%
!! ATGGTGGATTTGGGAGAAGA+TGG - tig0016604:15957-15976 Msa1358960:CDS 45.0%
GAGGAACACACAATTTGTGG+TGG - tig0016604:15997-16016 Msa1358960:CDS 45.0%
!!! AGGAGCTACTGTTTTTGGCA+TGG + tig0016604:16065-16084 None:intergenic 45.0%
TCAAGATCACCAGACAAAGC+AGG + tig0016604:16085-16104 None:intergenic 45.0%
GCTTTGTCTGGTGATCTTGA+TGG - tig0016604:16085-16104 Msa1358960:CDS 45.0%
! AAGATGGATGGAGATGATGG+TGG - tig0016604:16148-16167 Msa1358960:CDS 45.0%
GTGATGCCAACTAGGCCTAA+TGG - tig0016604:15938-15957 Msa1358960:CDS 50.0%
ATGCCAACTAGGCCTAATGG+TGG - tig0016604:15941-15960 Msa1358960:CDS 50.0%
ACAGTAGCTCCTGCTTTGTC+TGG - tig0016604:16073-16092 Msa1358960:CDS 50.0%
!! GATGGAGATGATGGTGGTGA+TGG - tig0016604:16154-16173 Msa1358960:CDS 50.0%
Chromosome Type Strat End Strand Name
tig0016604 gene 15635 16267 15635 ID=Msa1358960;Name=Msa1358960
tig0016604 mRNA 15635 16267 15635 ID=Msa1358960-mRNA-1;Parent=Msa1358960;Name=Msa1358960-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|210
tig0016604 exon 15635 16267 15635 ID=Msa1358960-mRNA-1:exon:11154;Parent=Msa1358960-mRNA-1
tig0016604 CDS 15635 16267 15635 ID=Msa1358960-mRNA-1:cds;Parent=Msa1358960-mRNA-1
Gene Sequence

>Msa1358960

ATGGATTGGTTTTCATGGCTATCAAAAACCAACCTTGAGCCATCTCTTGTATATGAGTATGGCCTTGTCTTTGCACAAAATGAGCTTGAAGAAGAAGACATGTTTTACTTCAACCATGAGTTTCTACAAAGCATGGGAATCTCCATAGCCAAACACAGATTAGAAATTCTCAAGCTTGCTAAAAAAGACAAGAGAAAAAGTCCACACACAGTTGCAAAATTTGTAGCTGCAATCAAGAGGACAAAGAAGTACTTAGCTAACTATGTTAGGACTTTGACTCATACTGAAGAATCAGCACTTATGGTGATGCCAACTAGGCCTAATGGTGGATTTGGGAGAAGATGGAAGAGTGCTATAATGAAGAGGAACACACAATTTGTGGTGGCTAAGCAAGAGAAAATGTTTCTCACAAATGGAAGGTCTTCTTCCATGCCAAAAACAGTAGCTCCTGCTTTGTCTGGTGATCTTGATGGTTTTTCTAGTCCTGTTGTGTATCATTTCCAAAAGGAGCAAAAGATGGATGGAGATGATGGTGGTGATGGTAATAATGATAATCATGATGATGGTTATTGGTCTGCTGCTGTTGAAGAGATTAAGTGGGATACCATGTTTAAGGATCTAAAGCCAAACTAA

Protein sequence

>Msa1358960

MDWFSWLSKTNLEPSLVYEYGLVFAQNELEEEDMFYFNHEFLQSMGISIAKHRLEILKLAKKDKRKSPHTVAKFVAAIKRTKKYLANYVRTLTHTEESALMVMPTRPNGGFGRRWKSAIMKRNTQFVVAKQEKMFLTNGRSSSMPKTVAPALSGDLDGFSSPVVYHFQKEQKMDGDDGGDGNNDNHDDGYWSAAVEEIKWDTMFKDLKPN*