AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1367260


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1367260 MtrunA17_Chr4g0074781 93.750 176 11 0 1 176 1 176 1.01e-120 338
Msa1367260 MtrunA17_Chr2g0288341 51.142 219 63 7 1 176 1 218 1.35e-55 174
Msa1367260 MtrunA17_Chr7g0264131 37.705 183 82 8 1 175 1 159 7.17e-21 84.0
Msa1367260 MtrunA17_Chr2g0326661 55.952 84 30 2 97 174 72 154 3.91e-20 82.0
Msa1367260 MtrunA17_Chr4g0029031 51.190 84 35 2 96 174 75 157 4.72e-18 76.6
Msa1367260 MtrunA17_Chr2g0288501 50.602 83 36 3 97 174 72 154 1.38e-16 72.8
Msa1367260 MtrunA17_Chr2g0321611 48.193 83 35 3 99 173 170 252 1.05e-14 70.1
Msa1367260 MtrunA17_Chr3g0099101 28.415 183 83 5 32 174 22 196 3.14e-14 67.4
Msa1367260 MtrunA17_Chr7g0266691 65.217 46 13 1 132 174 170 215 5.98e-12 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1367260 AT1G11700.1 47.087 206 74 7 1 176 1 201 3.07e-45 148
Msa1367260 AT1G61930.1 48.529 204 76 9 1 176 1 203 4.27e-43 142
Msa1367260 AT4G21930.1 46.193 197 71 9 1 176 1 183 3.40e-38 129
Msa1367260 AT3G45210.1 56.757 74 30 2 101 174 75 146 8.31e-21 84.0
Msa1367260 AT5G60680.1 35.519 183 87 4 1 174 1 161 1.66e-19 80.9
Msa1367260 AT4G21970.1 47.059 85 37 3 98 174 59 143 8.55e-19 79.0
Msa1367260 AT5G03230.1 46.591 88 34 2 99 174 78 164 2.90e-17 75.1
Msa1367260 AT2G28400.1 48.889 90 35 3 96 175 73 161 4.01e-17 74.7
Msa1367260 AT4G04630.1 48.315 89 34 3 98 174 78 166 1.25e-14 68.2
Msa1367260 AT4G26950.1 34.146 164 76 8 21 174 1 142 9.27e-14 65.5
Msa1367260 AT4G26950.2 34.146 164 76 8 21 174 1 142 9.27e-14 65.5
Msa1367260 AT3G15040.1 59.184 49 17 1 129 174 193 241 3.50e-12 63.2
Msa1367260 AT1G29640.1 47.561 82 31 4 97 174 64 137 7.26e-12 60.5

Find 59 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCATGAAGTAACCGATTTC+AGG 0.232378 tig0018511:+207065 Msa1367260:CDS
GTCGCAAGCGTTTGAGAATC+AGG 0.306206 tig0018511:+207195 Msa1367260:CDS
CAATTTCTCTCCCGGCGATT+GGG 0.307565 tig0018511:+207132 Msa1367260:CDS
GGTTCCGCCGCATGAGTATT+TGG 0.311065 tig0018511:+207375 Msa1367260:CDS
TTTGGCGCGTGGCCGGAAAA+TGG 0.357971 tig0018511:+207393 Msa1367260:CDS
CCGGAAAATGGCGGCAAATT+CGG 0.361903 tig0018511:+207405 Msa1367260:CDS
CACATCTTCTTCCCTGAAAT+CGG 0.365661 tig0018511:-207077 None:intergenic
AACCGAAACGAACGGTTGTT+AGG 0.366911 tig0018511:+207013 Msa1367260:CDS
CAACCGTTCGTTTCGGTTGG+TGG 0.379141 tig0018511:-207008 None:intergenic
GGTGGAATCGTTGCATGATA+TGG 0.383426 tig0018511:+207330 Msa1367260:CDS
GGGGTGGGTCGGACCTTAAA+AGG 0.389495 tig0018511:+207436 Msa1367260:CDS
TTCCTAACAACCGTTCGTTT+CGG 0.395593 tig0018511:-207015 None:intergenic
GTGCTGATGTTGCAACGTTC+CGG 0.398619 tig0018511:-207264 None:intergenic
TCAATTTCTCTCCCGGCGAT+TGG 0.413239 tig0018511:+207131 Msa1367260:CDS
GACGGGTTCGTGATGCTGTT+TGG 0.413527 tig0018511:+207470 Msa1367260:CDS
CTCCAATCCGGCACGTTCAC+CGG 0.413829 tig0018511:-207286 None:intergenic
CTCAACCATCGAACCCGGTT+TGG 0.416497 tig0018511:-207495 None:intergenic
GATGCTGTTTGGAACCAAAC+CGG 0.441060 tig0018511:+207481 Msa1367260:CDS
ATGTTGCAACGTTCCGGTAC+TGG 0.447319 tig0018511:-207258 None:intergenic
CACCGGTGAACGTGCCGGAT+TGG 0.454006 tig0018511:+207284 Msa1367260:CDS
GAATCGTTCACCACCAGTAC+CGG 0.485009 tig0018511:+207245 Msa1367260:CDS
ATGAGTATTTGGCGCGTGGC+CGG 0.490622 tig0018511:+207386 Msa1367260:CDS
ATGCTGTTTGGAACCAAACC+GGG 0.500659 tig0018511:+207482 Msa1367260:CDS
CGGAGTATCTTACTCCAATC+CGG 0.504693 tig0018511:-207298 None:intergenic
TAAGATACTCCGAGTTGAAT+CGG 0.506960 tig0018511:+207309 Msa1367260:CDS
TCATGAAGTAACCGATTTCA+GGG 0.508081 tig0018511:+207066 Msa1367260:CDS
TAACAACCGTTCGTTTCGGT+TGG 0.515395 tig0018511:-207011 None:intergenic
TGCGCGTGAATCCCAATCGC+CGG 0.518977 tig0018511:-207143 None:intergenic
GATTCTCAAACGCTTGCGAC+AGG 0.525300 tig0018511:-207192 None:intergenic
AAAAGGCCGTGATTTGAGAC+GGG 0.530895 tig0018511:+207453 Msa1367260:CDS
TATGGATGATGATAACGAAT+CGG 0.540008 tig0018511:+207348 Msa1367260:CDS
TGGAACCAAACCGGGTTCGA+TGG 0.549508 tig0018511:+207490 Msa1367260:CDS
CACAACGTCAATTTCTCTCC+CGG 0.549711 tig0018511:+207124 Msa1367260:CDS
TAAAAGGCCGTGATTTGAGA+CGG 0.552056 tig0018511:+207452 Msa1367260:CDS
GGTTACTTCATGAGTGGTGG+TGG 0.557686 tig0018511:-207056 None:intergenic
GTCGCTAAGTACCATGGCGA+AGG 0.565479 tig0018511:+206973 None:intergenic
CACGAACCCGTCTCAAATCA+CGG 0.568944 tig0018511:-207459 None:intergenic
TGATGATAACGAATCGGAGA+TGG 0.569605 tig0018511:+207354 Msa1367260:CDS
CAAACCGGGTTCGATGGTTG+AGG 0.576026 tig0018511:+207496 Msa1367260:CDS
GTGGTCACGATCTTCAACCA+TGG 0.577498 tig0018511:-207104 None:intergenic
CGCGCCAAATACTCATGCGG+CGG 0.577795 tig0018511:-207379 None:intergenic
ATCAGCACCGGTGAACGTGC+CGG 0.584228 tig0018511:+207279 Msa1367260:CDS
TCCACCTCAACCATCGAACC+CGG 0.586804 tig0018511:-207500 None:intergenic
GGCGCGTGGCCGGAAAATGG+CGG 0.591649 tig0018511:+207396 Msa1367260:CDS
GAATTTGCGACCCTTCGCCA+TGG 0.599300 tig0018511:-206984 None:intergenic
CCGCATGAGTATTTGGCGCG+TGG 0.607155 tig0018511:+207382 Msa1367260:CDS
GAACGTTGCAACATCAGCAC+CGG 0.627010 tig0018511:+207267 Msa1367260:CDS
GGAAGAAGATGTGTGGTCCA+TGG 0.635452 tig0018511:+207087 Msa1367260:CDS
ACGATTCCACCGATTCAACT+CGG 0.637845 tig0018511:-207318 None:intergenic
TCGCTAAGTACCATGGCGAA+GGG 0.643376 tig0018511:+206974 None:intergenic
CCACGCGCCAAATACTCATG+CGG 0.647845 tig0018511:-207382 None:intergenic
ATCGGTTACTTCATGAGTGG+TGG 0.648620 tig0018511:-207059 None:intergenic
CATTCACGTCGTCACAACCA+CGG 0.652333 tig0018511:+207166 Msa1367260:CDS
CGGGAGAGAAATTGACGTTG+TGG 0.653823 tig0018511:-207123 None:intergenic
TCACCACCAACCGAAACGAA+CGG 0.658770 tig0018511:+207005 Msa1367260:CDS
TTGCAACGTTCCGGTACTGG+TGG 0.660475 tig0018511:-207255 None:intergenic
GATACTCCGAGTTGAATCGG+TGG 0.676763 tig0018511:+207312 Msa1367260:CDS
GAAATCGGTTACTTCATGAG+TGG 0.682994 tig0018511:-207062 None:intergenic
ATTTCAGGGAAGAAGATGTG+TGG 0.693930 tig0018511:+207080 Msa1367260:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TATGGATGATGATAACGAAT+CGG + tig0018511:207348-207367 Msa1367260:CDS 30.0%
! TCATGAAGTAACCGATTTCA+GGG + tig0018511:207066-207085 Msa1367260:CDS 35.0%
! TAAGATACTCCGAGTTGAAT+CGG + tig0018511:207309-207328 Msa1367260:CDS 35.0%
TTCCTAACAACCGTTCGTTT+CGG - tig0018511:207018-207037 None:intergenic 40.0%
GAAATCGGTTACTTCATGAG+TGG - tig0018511:207065-207084 None:intergenic 40.0%
! CTCATGAAGTAACCGATTTC+AGG + tig0018511:207065-207084 Msa1367260:CDS 40.0%
CACATCTTCTTCCCTGAAAT+CGG - tig0018511:207080-207099 None:intergenic 40.0%
ATTTCAGGGAAGAAGATGTG+TGG + tig0018511:207080-207099 Msa1367260:CDS 40.0%
! TGATGATAACGAATCGGAGA+TGG + tig0018511:207354-207373 Msa1367260:CDS 40.0%
! TCTCAAATCACGGCCTTTTA+AGG - tig0018511:207452-207471 None:intergenic 40.0%
TAAAAGGCCGTGATTTGAGA+CGG + tig0018511:207452-207471 Msa1367260:CDS 40.0%
TAACAACCGTTCGTTTCGGT+TGG - tig0018511:207014-207033 None:intergenic 45.0%
AACCGAAACGAACGGTTGTT+AGG + tig0018511:207013-207032 Msa1367260:CDS 45.0%
ATCGGTTACTTCATGAGTGG+TGG - tig0018511:207062-207081 None:intergenic 45.0%
CACAACGTCAATTTCTCTCC+CGG + tig0018511:207124-207143 Msa1367260:CDS 45.0%
! CGGAGTATCTTACTCCAATC+CGG - tig0018511:207301-207320 None:intergenic 45.0%
!! ACGATTCCACCGATTCAACT+CGG - tig0018511:207321-207340 None:intergenic 45.0%
GGTGGAATCGTTGCATGATA+TGG + tig0018511:207330-207349 Msa1367260:CDS 45.0%
! CGGCAAATTCGGTTTTCGAA+GGG + tig0018511:207416-207435 Msa1367260:CDS 45.0%
! GGCAAATTCGGTTTTCGAAG+GGG + tig0018511:207417-207436 Msa1367260:CDS 45.0%
! AAATTCGGTTTTCGAAGGGG+TGG + tig0018511:207420-207439 Msa1367260:CDS 45.0%
! AATTCGGTTTTCGAAGGGGT+GGG + tig0018511:207421-207440 Msa1367260:CDS 45.0%
! AAAAGGCCGTGATTTGAGAC+GGG + tig0018511:207453-207472 Msa1367260:CDS 45.0%
! GATGCTGTTTGGAACCAAAC+CGG + tig0018511:207481-207500 Msa1367260:CDS 45.0%
! ATGCTGTTTGGAACCAAACC+GGG + tig0018511:207482-207501 Msa1367260:CDS 45.0%
TCACCACCAACCGAAACGAA+CGG + tig0018511:207005-207024 Msa1367260:CDS 50.0%
GGTTACTTCATGAGTGGTGG+TGG - tig0018511:207059-207078 None:intergenic 50.0%
GGAAGAAGATGTGTGGTCCA+TGG + tig0018511:207087-207106 Msa1367260:CDS 50.0%
GTGGTCACGATCTTCAACCA+TGG - tig0018511:207107-207126 None:intergenic 50.0%
CGGGAGAGAAATTGACGTTG+TGG - tig0018511:207126-207145 None:intergenic 50.0%
TCAATTTCTCTCCCGGCGAT+TGG + tig0018511:207131-207150 Msa1367260:CDS 50.0%
CAATTTCTCTCCCGGCGATT+GGG + tig0018511:207132-207151 Msa1367260:CDS 50.0%
CATTCACGTCGTCACAACCA+CGG + tig0018511:207166-207185 Msa1367260:CDS 50.0%
GATTCTCAAACGCTTGCGAC+AGG - tig0018511:207195-207214 None:intergenic 50.0%
GTCGCAAGCGTTTGAGAATC+AGG + tig0018511:207195-207214 Msa1367260:CDS 50.0%
GAATCGTTCACCACCAGTAC+CGG + tig0018511:207245-207264 Msa1367260:CDS 50.0%
ATGTTGCAACGTTCCGGTAC+TGG - tig0018511:207261-207280 None:intergenic 50.0%
! GTGCTGATGTTGCAACGTTC+CGG - tig0018511:207267-207286 None:intergenic 50.0%
GAACGTTGCAACATCAGCAC+CGG + tig0018511:207267-207286 Msa1367260:CDS 50.0%
! GATACTCCGAGTTGAATCGG+TGG + tig0018511:207312-207331 Msa1367260:CDS 50.0%
!! CCGAATTTGCCGCCATTTTC+CGG - tig0018511:207408-207427 None:intergenic 50.0%
CCGGAAAATGGCGGCAAATT+CGG + tig0018511:207405-207424 Msa1367260:CDS 50.0%
! GCGGCAAATTCGGTTTTCGA+AGG + tig0018511:207415-207434 Msa1367260:CDS 50.0%
CACGAACCCGTCTCAAATCA+CGG - tig0018511:207462-207481 None:intergenic 50.0%
CAACCGTTCGTTTCGGTTGG+TGG - tig0018511:207011-207030 None:intergenic 55.0%
! TTGCAACGTTCCGGTACTGG+TGG - tig0018511:207258-207277 None:intergenic 55.0%
GGTTCCGCCGCATGAGTATT+TGG + tig0018511:207375-207394 Msa1367260:CDS 55.0%
CCACGCGCCAAATACTCATG+CGG - tig0018511:207385-207404 None:intergenic 55.0%
ATGAGTATTTGGCGCGTGGC+CGG + tig0018511:207386-207405 Msa1367260:CDS 55.0%
! GACGGGTTCGTGATGCTGTT+TGG + tig0018511:207470-207489 Msa1367260:CDS 55.0%
TGGAACCAAACCGGGTTCGA+TGG + tig0018511:207490-207509 Msa1367260:CDS 55.0%
TGCGCGTGAATCCCAATCGC+CGG - tig0018511:207146-207165 None:intergenic 60.0%
TCACGTCGTCACAACCACGG+CGG + tig0018511:207169-207188 Msa1367260:CDS 60.0%
!! ATCAGCACCGGTGAACGTGC+CGG + tig0018511:207279-207298 Msa1367260:CDS 60.0%
CTCCAATCCGGCACGTTCAC+CGG - tig0018511:207289-207308 None:intergenic 60.0%
CGCGCCAAATACTCATGCGG+CGG - tig0018511:207382-207401 None:intergenic 60.0%
CCGCATGAGTATTTGGCGCG+TGG + tig0018511:207382-207401 Msa1367260:CDS 60.0%
TTTGGCGCGTGGCCGGAAAA+TGG + tig0018511:207393-207412 Msa1367260:CDS 60.0%
! CGGTTTTCGAAGGGGTGGGT+CGG + tig0018511:207425-207444 Msa1367260:CDS 60.0%
GGGGTGGGTCGGACCTTAAA+AGG + tig0018511:207436-207455 Msa1367260:CDS 60.0%
GCGCGTGAATCCCAATCGCC+GGG - tig0018511:207145-207164 None:intergenic 65.0%
CACCGGTGAACGTGCCGGAT+TGG + tig0018511:207284-207303 Msa1367260:CDS 65.0%
GGCGCGTGGCCGGAAAATGG+CGG + tig0018511:207396-207415 Msa1367260:CDS 70.0%
ACGCTTGCGACAGGCCGCCG+TGG - tig0018511:207186-207205 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
tig0018511 gene 206986 207516 206986 ID=Msa1367260;Name=Msa1367260
tig0018511 mRNA 206986 207516 206986 ID=Msa1367260-mRNA-1;Parent=Msa1367260;Name=Msa1367260-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|176
tig0018511 exon 206986 207516 206986 ID=Msa1367260-mRNA-1:exon:15380;Parent=Msa1367260-mRNA-1
tig0018511 CDS 206986 207516 206986 ID=Msa1367260-mRNA-1:cds;Parent=Msa1367260-mRNA-1
Gene Sequence

>Msa1367260

ATGGCGAAGGGTCGCAAATTCACCACCAACCGAAACGAACGGTTGTTAGGAACAAACTATATTCAAAGCTCCACCACCACTCATGAAGTAACCGATTTCAGGGAAGAAGATGTGTGGTCCATGGTTGAAGATCGTGACCACAACGTCAATTTCTCTCCCGGCGATTGGGATTCACGCGCACATTCACGTCGTCACAACCACGGCGGCCTGTCGCAAGCGTTTGAGAATCAGGTTAGTGATGTTGCGACTATGTCGTCGAGAATCGTTCACCACCAGTACCGGAACGTTGCAACATCAGCACCGGTGAACGTGCCGGATTGGAGTAAGATACTCCGAGTTGAATCGGTGGAATCGTTGCATGATATGGATGATGATAACGAATCGGAGATGGTTCCGCCGCATGAGTATTTGGCGCGTGGCCGGAAAATGGCGGCAAATTCGGTTTTCGAAGGGGTGGGTCGGACCTTAAAAGGCCGTGATTTGAGACGGGTTCGTGATGCTGTTTGGAACCAAACCGGGTTCGATGGTTGA

Protein sequence

>Msa1367260

MAKGRKFTTNRNERLLGTNYIQSSTTTHEVTDFREEDVWSMVEDRDHNVNFSPGDWDSRAHSRRHNHGGLSQAFENQVSDVATMSSRIVHHQYRNVATSAPVNVPDWSKILRVESVESLHDMDDDNESEMVPPHEYLARGRKMAANSVFEGVGRTLKGRDLRRVRDAVWNQTGFDG*