Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07433.t1 | XP_013468793.1 | 96.2 | 133 | 3 | 1 | 3 | 135 | 2 | 132 | 4.70E-66 | 260.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07433.t1 | A0A072VXN1 | 96.2 | 133 | 3 | 1 | 3 | 135 | 2 | 132 | 3.4e-66 | 260.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene07433 | 0.820326 | 7.28E-53 | -1.69E-46 |
| MS.gene049351 | MS.gene07433 | 0.840982 | 6.26E-58 | -1.69E-46 |
| MS.gene049356 | MS.gene07433 | 0.848926 | 4.49E-60 | -1.69E-46 |
| MS.gene049369 | MS.gene07433 | 0.84587 | 3.10E-59 | -1.69E-46 |
| MS.gene049609 | MS.gene07433 | 0.870084 | 1.91E-66 | -1.69E-46 |
| MS.gene049661 | MS.gene07433 | 0.831745 | 1.40E-55 | -1.69E-46 |
| MS.gene049662 | MS.gene07433 | 0.814895 | 1.22E-51 | -1.69E-46 |
| MS.gene049805 | MS.gene07433 | 0.848553 | 5.70E-60 | -1.69E-46 |
| MS.gene049907 | MS.gene07433 | 0.81032 | 1.22E-50 | -1.69E-46 |
| MS.gene050122 | MS.gene07433 | 0.829579 | 4.76E-55 | -1.69E-46 |
| MS.gene050397 | MS.gene07433 | 0.802857 | 4.60E-49 | -1.69E-46 |
| MS.gene050432 | MS.gene07433 | 0.832774 | 7.80E-56 | -1.69E-46 |
| MS.gene050499 | MS.gene07433 | 0.810439 | 1.15E-50 | -1.69E-46 |
| MS.gene050520 | MS.gene07433 | 0.85839 | 8.51E-63 | -1.69E-46 |
| MS.gene050593 | MS.gene07433 | 0.824845 | 6.47E-54 | -1.69E-46 |
| MS.gene050670 | MS.gene07433 | 0.850539 | 1.59E-60 | -1.69E-46 |
| MS.gene050686 | MS.gene07433 | 0.828126 | 1.07E-54 | -1.69E-46 |
| MS.gene050747 | MS.gene07433 | 0.814047 | 1.88E-51 | -1.69E-46 |
| MS.gene050767 | MS.gene07433 | 0.813452 | 2.54E-51 | -1.69E-46 |
| MS.gene050770 | MS.gene07433 | 0.814254 | 1.69E-51 | -1.69E-46 |
| MS.gene050772 | MS.gene07433 | 0.872893 | 2.24E-67 | -1.69E-46 |
| MS.gene050950 | MS.gene07433 | 0.807974 | 3.89E-50 | -1.69E-46 |
| MS.gene05105 | MS.gene07433 | 0.893827 | 4.37E-75 | -1.69E-46 |
| MS.gene051255 | MS.gene07433 | 0.854945 | 8.78E-62 | -1.69E-46 |
| MS.gene051360 | MS.gene07433 | 0.813662 | 2.29E-51 | -1.69E-46 |
| MS.gene051387 | MS.gene07433 | 0.83851 | 2.76E-57 | -1.69E-46 |
| MS.gene051666 | MS.gene07433 | 0.812353 | 4.42E-51 | -1.69E-46 |
| MS.gene051692 | MS.gene07433 | 0.853261 | 2.69E-61 | -1.69E-46 |
| MS.gene051810 | MS.gene07433 | 0.820064 | 8.36E-53 | -1.69E-46 |
| MS.gene051838 | MS.gene07433 | 0.806529 | 7.87E-50 | -1.69E-46 |
| MS.gene051858 | MS.gene07433 | 0.833761 | 4.43E-56 | -1.69E-46 |
| MS.gene052078 | MS.gene07433 | 0.802576 | 5.25E-49 | -1.69E-46 |
| MS.gene052188 | MS.gene07433 | 0.825746 | 3.96E-54 | -1.69E-46 |
| MS.gene052253 | MS.gene07433 | 0.832902 | 7.25E-56 | -1.69E-46 |
| MS.gene052271 | MS.gene07433 | 0.813135 | 2.98E-51 | -1.69E-46 |
| MS.gene052310 | MS.gene07433 | 0.816182 | 6.31E-52 | -1.69E-46 |
| MS.gene052322 | MS.gene07433 | 0.868869 | 4.74E-66 | -1.69E-46 |
| MS.gene052340 | MS.gene07433 | 0.80011 | 1.68E-48 | -1.69E-46 |
| MS.gene052346 | MS.gene07433 | 0.815836 | 7.54E-52 | -1.69E-46 |
| MS.gene052414 | MS.gene07433 | 0.818843 | 1.59E-52 | -1.69E-46 |
| MS.gene052421 | MS.gene07433 | 0.856803 | 2.51E-62 | -1.69E-46 |
| MS.gene052481 | MS.gene07433 | 0.832313 | 1.01E-55 | -1.69E-46 |
| MS.gene052484 | MS.gene07433 | 0.844077 | 9.45E-59 | -1.69E-46 |
| MS.gene052485 | MS.gene07433 | 0.847991 | 8.14E-60 | -1.69E-46 |
| MS.gene052615 | MS.gene07433 | 0.873622 | 1.28E-67 | -1.69E-46 |
| MS.gene052982 | MS.gene07433 | 0.858098 | 1.04E-62 | -1.69E-46 |
| MS.gene052999 | MS.gene07433 | 0.830859 | 2.32E-55 | -1.69E-46 |
| MS.gene053009 | MS.gene07433 | 0.825995 | 3.46E-54 | -1.69E-46 |
| MS.gene053103 | MS.gene07433 | 0.803921 | 2.77E-49 | -1.69E-46 |
| MS.gene053169 | MS.gene07433 | 0.803099 | 4.10E-49 | -1.69E-46 |
| MS.gene053207 | MS.gene07433 | 0.872715 | 2.57E-67 | -1.69E-46 |
| MS.gene053209 | MS.gene07433 | 0.873307 | 1.63E-67 | -1.69E-46 |
| MS.gene053213 | MS.gene07433 | 0.841828 | 3.75E-58 | -1.69E-46 |
| MS.gene053286 | MS.gene07433 | 0.830115 | 3.52E-55 | -1.69E-46 |
| MS.gene053322 | MS.gene07433 | 0.816656 | 4.94E-52 | -1.69E-46 |
| MS.gene053449 | MS.gene07433 | 0.84806 | 7.79E-60 | -1.69E-46 |
| MS.gene053574 | MS.gene07433 | 0.832564 | 8.80E-56 | -1.69E-46 |
| MS.gene053632 | MS.gene07433 | 0.83221 | 1.08E-55 | -1.69E-46 |
| MS.gene053758 | MS.gene07433 | 0.828816 | 7.28E-55 | -1.69E-46 |
| MS.gene053813 | MS.gene07433 | 0.853783 | 1.90E-61 | -1.69E-46 |
| MS.gene053824 | MS.gene07433 | 0.820605 | 6.28E-53 | -1.69E-46 |
| MS.gene053912 | MS.gene07433 | 0.824674 | 7.10E-54 | -1.69E-46 |
| MS.gene053916 | MS.gene07433 | 0.834188 | 3.46E-56 | -1.69E-46 |
| MS.gene054376 | MS.gene07433 | 0.80955 | 1.79E-50 | -1.69E-46 |
| MS.gene054600 | MS.gene07433 | 0.835922 | 1.27E-56 | -1.69E-46 |
| MS.gene054642 | MS.gene07433 | 0.809446 | 1.88E-50 | -1.69E-46 |
| MS.gene054804 | MS.gene07433 | 0.802968 | 4.36E-49 | -1.69E-46 |
| MS.gene054927 | MS.gene07433 | 0.825367 | 4.87E-54 | -1.69E-46 |
| MS.gene05494 | MS.gene07433 | 0.838055 | 3.61E-57 | -1.69E-46 |
| MS.gene05505 | MS.gene07433 | 0.866073 | 3.72E-65 | -1.69E-46 |
| MS.gene055116 | MS.gene07433 | 0.844819 | 5.97E-59 | -1.69E-46 |
| MS.gene055206 | MS.gene07433 | 0.82748 | 1.53E-54 | -1.69E-46 |
| MS.gene055224 | MS.gene07433 | 0.817405 | 3.35E-52 | -1.69E-46 |
| MS.gene055277 | MS.gene07433 | 0.819251 | 1.28E-52 | -1.69E-46 |
| MS.gene055289 | MS.gene07433 | 0.814669 | 1.37E-51 | -1.69E-46 |
| MS.gene055362 | MS.gene07433 | 0.805001 | 1.65E-49 | -1.69E-46 |
| MS.gene055412 | MS.gene07433 | 0.810431 | 1.16E-50 | -1.69E-46 |
| MS.gene055486 | MS.gene07433 | 0.8005 | 1.40E-48 | -1.69E-46 |
| MS.gene055499 | MS.gene07433 | 0.822655 | 2.11E-53 | -1.69E-46 |
| MS.gene055577 | MS.gene07433 | 0.804791 | 1.82E-49 | -1.69E-46 |
| MS.gene055647 | MS.gene07433 | 0.81429 | 1.66E-51 | -1.69E-46 |
| MS.gene055722 | MS.gene07433 | 0.85734 | 1.74E-62 | -1.69E-46 |
| MS.gene055741 | MS.gene07433 | 0.815663 | 8.24E-52 | -1.69E-46 |
| MS.gene055744 | MS.gene07433 | 0.814541 | 1.46E-51 | -1.69E-46 |
| MS.gene055909 | MS.gene07433 | 0.834337 | 3.18E-56 | -1.69E-46 |
| MS.gene055911 | MS.gene07433 | 0.835541 | 1.58E-56 | -1.69E-46 |
| MS.gene05592 | MS.gene07433 | 0.852558 | 4.27E-61 | -1.69E-46 |
| MS.gene055941 | MS.gene07433 | 0.823181 | 1.59E-53 | -1.69E-46 |
| MS.gene05601 | MS.gene07433 | 0.800039 | 1.74E-48 | -1.69E-46 |
| MS.gene05602 | MS.gene07433 | 0.823801 | 1.14E-53 | -1.69E-46 |
| MS.gene056191 | MS.gene07433 | 0.848831 | 4.77E-60 | -1.69E-46 |
| MS.gene056374 | MS.gene07433 | 0.805801 | 1.12E-49 | -1.69E-46 |
| MS.gene056407 | MS.gene07433 | 0.824703 | 6.99E-54 | -1.69E-46 |
| MS.gene056410 | MS.gene07433 | 0.82018 | 7.86E-53 | -1.69E-46 |
| MS.gene056422 | MS.gene07433 | 0.805594 | 1.24E-49 | -1.69E-46 |
| MS.gene056448 | MS.gene07433 | 0.80241 | 5.69E-49 | -1.69E-46 |
| MS.gene056511 | MS.gene07433 | 0.843652 | 1.23E-58 | -1.69E-46 |
| MS.gene056609 | MS.gene07433 | 0.812285 | 4.58E-51 | -1.69E-46 |
| MS.gene05664 | MS.gene07433 | 0.818261 | 2.15E-52 | -1.69E-46 |
| MS.gene056659 | MS.gene07433 | 0.821486 | 3.94E-53 | -1.69E-46 |
| MS.gene05666 | MS.gene07433 | 0.812903 | 3.35E-51 | -1.69E-46 |
| MS.gene056796 | MS.gene07433 | 0.81497 | 1.17E-51 | -1.69E-46 |
| MS.gene056897 | MS.gene07433 | 0.805839 | 1.10E-49 | -1.69E-46 |
| MS.gene05702 | MS.gene07433 | 0.812001 | 5.28E-51 | -1.69E-46 |
| MS.gene057076 | MS.gene07433 | 0.845292 | 4.45E-59 | -1.69E-46 |
| MS.gene057124 | MS.gene07433 | 0.881663 | 1.99E-70 | -1.69E-46 |
| MS.gene057129 | MS.gene07433 | 0.830044 | 3.67E-55 | -1.69E-46 |
| MS.gene057331 | MS.gene07433 | 0.850686 | 1.45E-60 | -1.69E-46 |
| MS.gene05735 | MS.gene07433 | 0.810579 | 1.07E-50 | -1.69E-46 |
| MS.gene057416 | MS.gene07433 | 0.851303 | 9.69E-61 | -1.69E-46 |
| MS.gene057734 | MS.gene07433 | 0.827282 | 1.70E-54 | -1.69E-46 |
| MS.gene057749 | MS.gene07433 | 0.845751 | 3.34E-59 | -1.69E-46 |
| MS.gene057823 | MS.gene07433 | 0.856147 | 3.92E-62 | -1.69E-46 |
| MS.gene058006 | MS.gene07433 | 0.856556 | 2.97E-62 | -1.69E-46 |
| MS.gene058019 | MS.gene07433 | 0.817493 | 3.21E-52 | -1.69E-46 |
| MS.gene058082 | MS.gene07433 | 0.808299 | 3.32E-50 | -1.69E-46 |
| MS.gene058120 | MS.gene07433 | 0.815921 | 7.22E-52 | -1.69E-46 |
| MS.gene058130 | MS.gene07433 | 0.844247 | 8.51E-59 | -1.69E-46 |
| MS.gene058293 | MS.gene07433 | 0.818805 | 1.62E-52 | -1.69E-46 |
| MS.gene058323 | MS.gene07433 | 0.83325 | 5.94E-56 | -1.69E-46 |
| MS.gene058660 | MS.gene07433 | 0.822309 | 2.54E-53 | -1.69E-46 |
| MS.gene058700 | MS.gene07433 | 0.822776 | 1.98E-53 | -1.69E-46 |
| MS.gene058723 | MS.gene07433 | 0.829504 | 4.96E-55 | -1.69E-46 |
| MS.gene058838 | MS.gene07433 | 0.801092 | 1.06E-48 | -1.69E-46 |
| MS.gene05886 | MS.gene07433 | 0.821618 | 3.67E-53 | -1.69E-46 |
| MS.gene058928 | MS.gene07433 | 0.829035 | 6.45E-55 | -1.69E-46 |
| MS.gene058948 | MS.gene07433 | 0.869453 | 3.06E-66 | -1.69E-46 |
| MS.gene058993 | MS.gene07433 | 0.826831 | 2.19E-54 | -1.69E-46 |
| MS.gene059023 | MS.gene07433 | 0.805279 | 1.44E-49 | -1.69E-46 |
| MS.gene059366 | MS.gene07433 | 0.83573 | 1.42E-56 | -1.69E-46 |
| MS.gene059367 | MS.gene07433 | 0.803835 | 2.88E-49 | -1.69E-46 |
| MS.gene059368 | MS.gene07433 | 0.834256 | 3.33E-56 | -1.69E-46 |
| MS.gene059420 | MS.gene07433 | 0.821111 | 4.80E-53 | -1.69E-46 |
| MS.gene059455 | MS.gene07433 | 0.814796 | 1.28E-51 | -1.69E-46 |
| MS.gene059536 | MS.gene07433 | 0.820221 | 7.69E-53 | -1.69E-46 |
| MS.gene059674 | MS.gene07433 | 0.832415 | 9.58E-56 | -1.69E-46 |
| MS.gene059676 | MS.gene07433 | 0.81865 | 1.75E-52 | -1.69E-46 |
| MS.gene059680 | MS.gene07433 | 0.814275 | 1.67E-51 | -1.69E-46 |
| MS.gene059688 | MS.gene07433 | 0.863688 | 2.08E-64 | -1.69E-46 |
| MS.gene059692 | MS.gene07433 | 0.857767 | 1.30E-62 | -1.69E-46 |
| MS.gene059695 | MS.gene07433 | 0.86567 | 4.99E-65 | -1.69E-46 |
| MS.gene059711 | MS.gene07433 | 0.856473 | 3.14E-62 | -1.69E-46 |
| MS.gene059718 | MS.gene07433 | 0.832312 | 1.02E-55 | -1.69E-46 |
| MS.gene059902 | MS.gene07433 | 0.831303 | 1.80E-55 | -1.69E-46 |
| MS.gene059974 | MS.gene07433 | 0.823738 | 1.18E-53 | -1.69E-46 |
| MS.gene060193 | MS.gene07433 | 0.806587 | 7.65E-50 | -1.69E-46 |
| MS.gene060356 | MS.gene07433 | 0.819023 | 1.44E-52 | -1.69E-46 |
| MS.gene060372 | MS.gene07433 | 0.829023 | 6.49E-55 | -1.69E-46 |
| MS.gene06037 | MS.gene07433 | 0.808836 | 2.55E-50 | -1.69E-46 |
| MS.gene060403 | MS.gene07433 | 0.83655 | 8.76E-57 | -1.69E-46 |
| MS.gene060469 | MS.gene07433 | 0.8246 | 7.39E-54 | -1.69E-46 |
| MS.gene060482 | MS.gene07433 | 0.818138 | 2.29E-52 | -1.69E-46 |
| MS.gene060804 | MS.gene07433 | 0.837673 | 4.53E-57 | -1.69E-46 |
| MS.gene060818 | MS.gene07433 | 0.814159 | 1.78E-51 | -1.69E-46 |
| MS.gene060835 | MS.gene07433 | 0.863088 | 3.20E-64 | -1.69E-46 |
| MS.gene060837 | MS.gene07433 | 0.89293 | 1.01E-74 | -1.69E-46 |
| MS.gene06092 | MS.gene07433 | 0.827022 | 1.97E-54 | -1.69E-46 |
| MS.gene061041 | MS.gene07433 | 0.820279 | 7.46E-53 | -1.69E-46 |
| MS.gene061049 | MS.gene07433 | 0.80561 | 1.23E-49 | -1.69E-46 |
| MS.gene061154 | MS.gene07433 | 0.845518 | 3.86E-59 | -1.69E-46 |
| MS.gene061200 | MS.gene07433 | 0.835285 | 1.83E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07433.t1 | MTR_1g076840 | 96.241 | 133 | 3 | 1 | 3 | 135 | 2 | 132 | 5.41e-91 | 259 |
| MS.gene07433.t1 | MTR_6g060170 | 82.474 | 97 | 17 | 0 | 39 | 135 | 2 | 98 | 1.70e-57 | 174 |
| MS.gene07433.t1 | MTR_1g069370 | 60.440 | 91 | 36 | 0 | 45 | 135 | 9 | 99 | 4.59e-38 | 125 |
| MS.gene07433.t1 | MTR_7g099200 | 59.341 | 91 | 37 | 0 | 45 | 135 | 5 | 95 | 2.70e-37 | 123 |
| MS.gene07433.t1 | MTR_6g059920 | 53.763 | 93 | 43 | 0 | 40 | 132 | 33 | 125 | 1.54e-30 | 106 |
| MS.gene07433.t1 | MTR_5g005820 | 51.087 | 92 | 45 | 0 | 40 | 131 | 2 | 93 | 2.45e-26 | 95.1 |
| MS.gene07433.t1 | MTR_6g060000 | 86.047 | 43 | 6 | 0 | 76 | 118 | 19 | 61 | 3.66e-22 | 83.6 |
| MS.gene07433.t1 | MTR_6g060090 | 68.000 | 50 | 14 | 1 | 68 | 117 | 9 | 56 | 2.21e-18 | 73.6 |
| MS.gene07433.t1 | MTR_1g086000 | 32.500 | 80 | 54 | 0 | 37 | 116 | 39 | 118 | 1.41e-12 | 61.2 |
| MS.gene07433.t1 | MTR_6g060030 | 55.172 | 58 | 8 | 1 | 96 | 135 | 7 | 64 | 1.63e-12 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07433.t1 | AT4G10300 | 67.669 | 133 | 39 | 3 | 6 | 135 | 3 | 134 | 1.03e-59 | 181 |
| MS.gene07433.t1 | AT3G04300 | 56.044 | 91 | 40 | 0 | 45 | 135 | 1 | 91 | 1.65e-35 | 118 |
| MS.gene07433.t1 | AT4G28703 | 48.454 | 97 | 41 | 1 | 47 | 134 | 8 | 104 | 4.92e-29 | 102 |
| MS.gene07433.t1 | AT4G10280 | 48.454 | 97 | 47 | 1 | 42 | 135 | 43 | 139 | 1.55e-26 | 97.1 |
| MS.gene07433.t1 | AT4G10290 | 43.617 | 94 | 50 | 1 | 42 | 132 | 15 | 108 | 3.28e-20 | 80.1 |
| MS.gene07433.t1 | AT2G32180 | 30.380 | 79 | 55 | 0 | 41 | 119 | 40 | 118 | 9.89e-12 | 58.9 |
| MS.gene07433.t1 | AT2G32180 | 30.380 | 79 | 55 | 0 | 41 | 119 | 40 | 118 | 9.89e-12 | 58.9 |
| MS.gene07433.t1 | AT2G32650 | 30.380 | 79 | 55 | 0 | 41 | 119 | 40 | 118 | 1.09e-11 | 58.5 |
| MS.gene07433.t1 | AT2G32650 | 30.380 | 79 | 55 | 0 | 41 | 119 | 40 | 118 | 1.09e-11 | 58.5 |
Find 34 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAGTACAACTCATCCCTTT+TGG | 0.324093 | 1.2:-63461989 | None:intergenic |
| TGAAATTTCTGCTGGTGATT+TGG | 0.359064 | 1.2:+63461958 | MS.gene07433:CDS |
| AACAGAGACATGCTATCTTT+TGG | 0.368243 | 1.2:+63461892 | MS.gene07433:CDS |
| CAAAAGGGATGAGTTGTACT+TGG | 0.379437 | 1.2:+63461990 | MS.gene07433:CDS |
| CTTGGTAGCAGTCAAATGTT+TGG | 0.389927 | 1.2:-63461284 | None:intergenic |
| ACAACTCTTCTCCTTGTTGA+AGG | 0.406999 | 1.2:-63461328 | None:intergenic |
| AGCTCACTCAACTTGGTGTT+AGG | 0.443465 | 1.2:+63461410 | MS.gene07433:CDS |
| AACACCAAGTTGAGTGAGCT+TGG | 0.455326 | 1.2:-63461407 | None:intergenic |
| AGTTGAGTGAGCTTGGACTC+AGG | 0.458878 | 1.2:-63461400 | None:intergenic |
| GGCAAAGTTACACTTACCTT+GGG | 0.470688 | 1.2:-63461307 | None:intergenic |
| CACTCCTAGTGGGGCTAATG+AGG | 0.472036 | 1.2:+63461931 | MS.gene07433:CDS |
| TATGTCCATGGAAAATTACT+TGG | 0.480875 | 1.2:-63461863 | None:intergenic |
| CTCAACTTGGTGTTAGGCAG+TGG | 0.492268 | 1.2:+63461416 | MS.gene07433:CDS |
| AGGCAAAGTTACACTTACCT+TGG | 0.501016 | 1.2:-63461308 | None:intergenic |
| TGTACTTGGGATGTGTCTGT+TGG | 0.525073 | 1.2:+63462004 | MS.gene07433:CDS |
| GAGTCCAAGCTCACTCAACT+TGG | 0.526860 | 1.2:+63461403 | MS.gene07433:CDS |
| AGTTACACTTACCTTGGGCT+TGG | 0.544555 | 1.2:-63461302 | None:intergenic |
| TTGGGATGTGTCTGTTGGTG+TGG | 0.547160 | 1.2:+63462009 | MS.gene07433:CDS |
| AGTCAAATGTTTGGTTTGTG+AGG | 0.547845 | 1.2:-63461275 | None:intergenic |
| GTCCATGGAAAATTACTTGG+AGG | 0.553197 | 1.2:-63461860 | None:intergenic |
| GCAGCTGTCACAGAGAAACT+TGG | 0.559175 | 1.2:+63461355 | MS.gene07433:CDS |
| AAGTGAAGGTCACTCCTAGT+GGG | 0.564562 | 1.2:+63461921 | MS.gene07433:CDS |
| TCCATGGAAAATTACTTGGA+GGG | 0.566600 | 1.2:-63461859 | None:intergenic |
| AAAGTGAAGGTCACTCCTAG+TGG | 0.587646 | 1.2:+63461920 | MS.gene07433:CDS |
| GAGGCAGTTGAAATTTCTGC+TGG | 0.589901 | 1.2:+63461950 | MS.gene07433:CDS |
| TGAGTGAGCTTGGACTCAGG+AGG | 0.606187 | 1.2:-63461397 | None:intergenic |
| AAAAGGGATGAGTTGTACTT+GGG | 0.609307 | 1.2:+63461991 | MS.gene07433:CDS |
| ATGGCATCATCATCATGTGT+AGG | 0.613363 | 1.2:+63461241 | MS.gene07433:CDS |
| GTGTAACTTTGCCTTCAACA+AGG | 0.618419 | 1.2:+63461317 | MS.gene07433:CDS |
| TCTGTTGCATCATATGTCCA+TGG | 0.622988 | 1.2:-63461875 | None:intergenic |
| GAGTGAGCTTGGACTCAGGA+GGG | 0.632998 | 1.2:-63461396 | None:intergenic |
| ACTGCCTCATTAGCCCCACT+AGG | 0.662701 | 1.2:-63461935 | None:intergenic |
| TTTGACTGCTACCAAGCCCA+AGG | 0.672230 | 1.2:+63461291 | MS.gene07433:CDS |
| AGTGAAGGTCACTCCTAGTG+GGG | 0.682734 | 1.2:+63461922 | MS.gene07433:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GATTTTTATTTTAATGAAAA+AGG | + | chr1.2:63461548-63461567 | MS.gene07433:intron | 10.0% |
| !! | ACAACATAAAAAATCTTTCA+CGG | + | chr1.2:63461749-63461768 | MS.gene07433:intron | 20.0% |
| !! | TACAGTTAAAGTCATATATA+TGG | - | chr1.2:63461672-63461691 | None:intergenic | 20.0% |
| !!! | AAACGTTTTTTATGATTTGA+CGG | + | chr1.2:63461707-63461726 | MS.gene07433:intron | 20.0% |
| ! | AAGAAATGTGGTATTTACTT+GGG | - | chr1.2:63461442-63461461 | None:intergenic | 25.0% |
| ! | AAGAAGATAATGAAGAAATG+TGG | - | chr1.2:63461454-63461473 | None:intergenic | 25.0% |
| ! | ATATGACTTTAACTGTAGTT+AGG | + | chr1.2:63461675-63461694 | MS.gene07433:intron | 25.0% |
| ! | TGGTGTAAATAAAAACAGAT+GGG | + | chr1.2:63461832-63461851 | MS.gene07433:intron | 25.0% |
| ! | TTAACGAGATTAATACACTA+TGG | + | chr1.2:63461626-63461645 | MS.gene07433:intron | 25.0% |
| ! | TTAGAATAATAGACATGATG+AGG | - | chr1.2:63461485-63461504 | None:intergenic | 25.0% |
| ! | TTGGTGTAAATAAAAACAGA+TGG | + | chr1.2:63461831-63461850 | MS.gene07433:intron | 25.0% |
| !!! | GTTTCTTACATTTTTTCTCA+TGG | + | chr1.2:63461513-63461532 | MS.gene07433:intron | 25.0% |
| GAAGAAATGTGGTATTTACT+TGG | - | chr1.2:63461443-63461462 | None:intergenic | 30.0% | |
| GGTGTAAATAAAAACAGATG+GGG | + | chr1.2:63461833-63461852 | MS.gene07433:intron | 30.0% | |
| TATGTCCATGGAAAATTACT+TGG | - | chr1.2:63461866-63461885 | None:intergenic | 30.0% | |
| !! | ATTTGGTTGTTTTCCCAAAA+GGG | + | chr1.2:63461975-63461994 | MS.gene07433:CDS | 30.0% |
| !!! | TCTTACATTTTTTCTCATGG+TGG | + | chr1.2:63461516-63461535 | MS.gene07433:intron | 30.0% |
| AAAAGGGATGAGTTGTACTT+GGG | + | chr1.2:63461991-63462010 | MS.gene07433:CDS | 35.0% | |
| TCCATGGAAAATTACTTGGA+GGG | - | chr1.2:63461862-63461881 | None:intergenic | 35.0% | |
| TGAAATTTCTGCTGGTGATT+TGG | + | chr1.2:63461958-63461977 | MS.gene07433:CDS | 35.0% | |
| ! | AACAGAGACATGCTATCTTT+TGG | + | chr1.2:63461892-63461911 | MS.gene07433:CDS | 35.0% |
| ! | AAGTACAACTCATCCCTTTT+GGG | - | chr1.2:63461991-63462010 | None:intergenic | 35.0% |
| !! | AGTCAAATGTTTGGTTTGTG+AGG | - | chr1.2:63461278-63461297 | None:intergenic | 35.0% |
| !! | GATTTGGTTGTTTTCCCAAA+AGG | + | chr1.2:63461974-63461993 | MS.gene07433:CDS | 35.0% |
| !! | TCTTTTGGAAGGAAAAGTGA+AGG | + | chr1.2:63461907-63461926 | MS.gene07433:CDS | 35.0% |
| ACAACTCTTCTCCTTGTTGA+AGG | - | chr1.2:63461331-63461350 | None:intergenic | 40.0% | |
| ACTAGAAGAGCAACTAGCAT+AGG | - | chr1.2:63461607-63461626 | None:intergenic | 40.0% | |
| AGGCAAAGTTACACTTACCT+TGG | - | chr1.2:63461311-63461330 | None:intergenic | 40.0% | |
| ATGGCATCATCATCATGTGT+AGG | + | chr1.2:63461241-63461260 | MS.gene07433:CDS | 40.0% | |
| CAAAAGGGATGAGTTGTACT+TGG | + | chr1.2:63461990-63462009 | MS.gene07433:CDS | 40.0% | |
| CTAGAAGAGCAACTAGCATA+GGG | - | chr1.2:63461606-63461625 | None:intergenic | 40.0% | |
| GGCAAAGTTACACTTACCTT+GGG | - | chr1.2:63461310-63461329 | None:intergenic | 40.0% | |
| GTCCATGGAAAATTACTTGG+AGG | - | chr1.2:63461863-63461882 | None:intergenic | 40.0% | |
| GTGTAACTTTGCCTTCAACA+AGG | + | chr1.2:63461317-63461336 | MS.gene07433:CDS | 40.0% | |
| TCTGTTGCATCATATGTCCA+TGG | - | chr1.2:63461878-63461897 | None:intergenic | 40.0% | |
| ! | CAAGTACAACTCATCCCTTT+TGG | - | chr1.2:63461992-63462011 | None:intergenic | 40.0% |
| ! | CTTGGTAGCAGTCAAATGTT+TGG | - | chr1.2:63461287-63461306 | None:intergenic | 40.0% |
| ! | GAGACATGCTATCTTTTGGA+AGG | + | chr1.2:63461896-63461915 | MS.gene07433:CDS | 40.0% |
| ! | TGACTGTGTACGCACATTTT+TGG | + | chr1.2:63461812-63461831 | MS.gene07433:intron | 40.0% |
| AAAGTGAAGGTCACTCCTAG+TGG | + | chr1.2:63461920-63461939 | MS.gene07433:CDS | 45.0% | |
| AAGTGAAGGTCACTCCTAGT+GGG | + | chr1.2:63461921-63461940 | MS.gene07433:CDS | 45.0% | |
| AGTTACACTTACCTTGGGCT+TGG | - | chr1.2:63461305-63461324 | None:intergenic | 45.0% | |
| GAGGCAGTTGAAATTTCTGC+TGG | + | chr1.2:63461950-63461969 | MS.gene07433:CDS | 45.0% | |
| TGTACTTGGGATGTGTCTGT+TGG | + | chr1.2:63462004-63462023 | MS.gene07433:CDS | 45.0% | |
| ! | AACACCAAGTTGAGTGAGCT+TGG | - | chr1.2:63461410-63461429 | None:intergenic | 45.0% |
| ! | GCCCTCCAAGTAATTTTCCA+TGG | + | chr1.2:63461858-63461877 | MS.gene07433:CDS | 45.0% |
| !! | AGCTCACTCAACTTGGTGTT+AGG | + | chr1.2:63461410-63461429 | MS.gene07433:CDS | 45.0% |
| AGTGAAGGTCACTCCTAGTG+GGG | + | chr1.2:63461922-63461941 | MS.gene07433:CDS | 50.0% | |
| AGTTGAGTGAGCTTGGACTC+AGG | - | chr1.2:63461403-63461422 | None:intergenic | 50.0% | |
| GAGTCCAAGCTCACTCAACT+TGG | + | chr1.2:63461403-63461422 | MS.gene07433:CDS | 50.0% | |
| GCAGCTGTCACAGAGAAACT+TGG | + | chr1.2:63461355-63461374 | MS.gene07433:CDS | 50.0% | |
| TTGGGATGTGTCTGTTGGTG+TGG | + | chr1.2:63462009-63462028 | MS.gene07433:CDS | 50.0% | |
| TTTGACTGCTACCAAGCCCA+AGG | + | chr1.2:63461291-63461310 | MS.gene07433:CDS | 50.0% | |
| !! | CTCAACTTGGTGTTAGGCAG+TGG | + | chr1.2:63461416-63461435 | MS.gene07433:CDS | 50.0% |
| ACTGCCTCATTAGCCCCACT+AGG | - | chr1.2:63461938-63461957 | None:intergenic | 55.0% | |
| CACTCCTAGTGGGGCTAATG+AGG | + | chr1.2:63461931-63461950 | MS.gene07433:CDS | 55.0% | |
| GAGTGAGCTTGGACTCAGGA+GGG | - | chr1.2:63461399-63461418 | None:intergenic | 55.0% | |
| TGAGTGAGCTTGGACTCAGG+AGG | - | chr1.2:63461400-63461419 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 63461241 | 63462054 | 63461241 | ID=MS.gene07433 |
| chr1.2 | mRNA | 63461241 | 63462054 | 63461241 | ID=MS.gene07433.t1;Parent=MS.gene07433 |
| chr1.2 | exon | 63461241 | 63461443 | 63461241 | ID=MS.gene07433.t1.exon1;Parent=MS.gene07433.t1 |
| chr1.2 | CDS | 63461241 | 63461443 | 63461241 | ID=cds.MS.gene07433.t1;Parent=MS.gene07433.t1 |
| chr1.2 | exon | 63461850 | 63462054 | 63461850 | ID=MS.gene07433.t1.exon2;Parent=MS.gene07433.t1 |
| chr1.2 | CDS | 63461850 | 63462054 | 63461850 | ID=cds.MS.gene07433.t1;Parent=MS.gene07433.t1 |
| Gene Sequence |
| Protein sequence |
