Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58826.t1 | XP_003624904.1 | 95.1 | 267 | 5 | 1 | 1 | 267 | 1 | 259 | 6.50E-136 | 493.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58826.t1 | Q9LXX5 | 78.9 | 213 | 44 | 1 | 56 | 267 | 50 | 262 | 8.1e-95 | 348.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58826.t1 | A2Q6H0 | 95.1 | 267 | 5 | 1 | 1 | 267 | 1 | 259 | 4.7e-136 | 493.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049137 | MS.gene58826 | 0.837415 | 5.27E-57 | -1.69E-46 |
| MS.gene049171 | MS.gene58826 | 0.858118 | 1.03E-62 | -1.69E-46 |
| MS.gene049173 | MS.gene58826 | 0.818337 | 2.07E-52 | -1.69E-46 |
| MS.gene049217 | MS.gene58826 | 0.827768 | 1.30E-54 | -1.69E-46 |
| MS.gene049222 | MS.gene58826 | 0.86263 | 4.42E-64 | -1.69E-46 |
| MS.gene049285 | MS.gene58826 | 0.893102 | 8.59E-75 | -1.69E-46 |
| MS.gene049320 | MS.gene58826 | 0.893788 | 4.54E-75 | -1.69E-46 |
| MS.gene049427 | MS.gene58826 | 0.817344 | 3.46E-52 | -1.69E-46 |
| MS.gene049609 | MS.gene58826 | 0.843207 | 1.61E-58 | -1.69E-46 |
| MS.gene049615 | MS.gene58826 | 0.828866 | 7.08E-55 | -1.69E-46 |
| MS.gene049627 | MS.gene58826 | 0.832724 | 8.03E-56 | -1.69E-46 |
| MS.gene049805 | MS.gene58826 | 0.836017 | 1.20E-56 | -1.69E-46 |
| MS.gene05010 | MS.gene58826 | 0.822903 | 1.85E-53 | -1.69E-46 |
| MS.gene050163 | MS.gene58826 | 0.895931 | 5.99E-76 | -1.69E-46 |
| MS.gene050165 | MS.gene58826 | 0.869989 | 2.05E-66 | -1.69E-46 |
| MS.gene050333 | MS.gene58826 | 0.845118 | 4.96E-59 | -1.69E-46 |
| MS.gene050504 | MS.gene58826 | 0.872747 | 2.51E-67 | -1.69E-46 |
| MS.gene050518 | MS.gene58826 | 0.84809 | 7.65E-60 | -1.69E-46 |
| MS.gene050538 | MS.gene58826 | 0.809476 | 1.86E-50 | -1.69E-46 |
| MS.gene050577 | MS.gene58826 | 0.862619 | 4.46E-64 | -1.69E-46 |
| MS.gene050593 | MS.gene58826 | 0.810058 | 1.39E-50 | -1.69E-46 |
| MS.gene050612 | MS.gene58826 | 0.840685 | 7.49E-58 | -1.69E-46 |
| MS.gene050675 | MS.gene58826 | 0.813925 | 2.00E-51 | -1.69E-46 |
| MS.gene050699 | MS.gene58826 | 0.884073 | 2.62E-71 | -1.69E-46 |
| MS.gene050747 | MS.gene58826 | 0.845822 | 3.20E-59 | -1.69E-46 |
| MS.gene050767 | MS.gene58826 | 0.822519 | 2.27E-53 | -1.69E-46 |
| MS.gene050770 | MS.gene58826 | 0.823666 | 1.23E-53 | -1.69E-46 |
| MS.gene050772 | MS.gene58826 | 0.860277 | 2.31E-63 | -1.69E-46 |
| MS.gene050781 | MS.gene58826 | 0.859936 | 2.93E-63 | -1.69E-46 |
| MS.gene050783 | MS.gene58826 | 0.819027 | 1.44E-52 | -1.69E-46 |
| MS.gene050950 | MS.gene58826 | 0.839615 | 1.43E-57 | -1.69E-46 |
| MS.gene050986 | MS.gene58826 | 0.847604 | 1.04E-59 | -1.69E-46 |
| MS.gene05100 | MS.gene58826 | 0.911763 | 4.21E-83 | -1.69E-46 |
| MS.gene051228 | MS.gene58826 | 0.803499 | 3.39E-49 | -1.69E-46 |
| MS.gene051551 | MS.gene58826 | 0.868701 | 5.37E-66 | -1.69E-46 |
| MS.gene051666 | MS.gene58826 | 0.843298 | 1.53E-58 | -1.69E-46 |
| MS.gene051692 | MS.gene58826 | 0.801475 | 8.85E-49 | -1.69E-46 |
| MS.gene051694 | MS.gene58826 | 0.82565 | 4.18E-54 | -1.69E-46 |
| MS.gene051810 | MS.gene58826 | 0.837435 | 5.21E-57 | -1.69E-46 |
| MS.gene051949 | MS.gene58826 | 0.802928 | 4.44E-49 | -1.69E-46 |
| MS.gene052164 | MS.gene58826 | 0.808826 | 2.56E-50 | -1.69E-46 |
| MS.gene052271 | MS.gene58826 | 0.843341 | 1.49E-58 | -1.69E-46 |
| MS.gene052379 | MS.gene58826 | 0.821025 | 5.03E-53 | -1.69E-46 |
| MS.gene052421 | MS.gene58826 | 0.802668 | 5.03E-49 | -1.69E-46 |
| MS.gene052486 | MS.gene58826 | 0.800154 | 1.65E-48 | -1.69E-46 |
| MS.gene052501 | MS.gene58826 | 0.818462 | 1.94E-52 | -1.69E-46 |
| MS.gene052503 | MS.gene58826 | 0.850619 | 1.51E-60 | -1.69E-46 |
| MS.gene052505 | MS.gene58826 | 0.800856 | 1.18E-48 | -1.69E-46 |
| MS.gene052615 | MS.gene58826 | 0.841465 | 4.67E-58 | -1.69E-46 |
| MS.gene052823 | MS.gene58826 | 0.846682 | 1.86E-59 | -1.69E-46 |
| MS.gene052825 | MS.gene58826 | 0.841493 | 4.60E-58 | -1.69E-46 |
| MS.gene052916 | MS.gene58826 | 0.804024 | 2.63E-49 | -1.69E-46 |
| MS.gene053103 | MS.gene58826 | 0.801532 | 8.61E-49 | -1.69E-46 |
| MS.gene053104 | MS.gene58826 | 0.808878 | 2.49E-50 | -1.69E-46 |
| MS.gene053203 | MS.gene58826 | 0.817647 | 2.96E-52 | -1.69E-46 |
| MS.gene053207 | MS.gene58826 | 0.828095 | 1.09E-54 | -1.69E-46 |
| MS.gene053213 | MS.gene58826 | 0.905127 | 5.96E-80 | -1.69E-46 |
| MS.gene053286 | MS.gene58826 | 0.900991 | 4.20E-78 | -1.69E-46 |
| MS.gene053322 | MS.gene58826 | 0.856622 | 2.84E-62 | -1.69E-46 |
| MS.gene053436 | MS.gene58826 | 0.856695 | 2.70E-62 | -1.69E-46 |
| MS.gene053480 | MS.gene58826 | 0.808478 | 3.04E-50 | -1.69E-46 |
| MS.gene053574 | MS.gene58826 | 0.82577 | 3.91E-54 | -1.69E-46 |
| MS.gene053579 | MS.gene58826 | 0.808461 | 3.06E-50 | -1.69E-46 |
| MS.gene053632 | MS.gene58826 | 0.878722 | 2.23E-69 | -1.69E-46 |
| MS.gene054376 | MS.gene58826 | 0.80252 | 5.40E-49 | -1.69E-46 |
| MS.gene054408 | MS.gene58826 | 0.843992 | 9.96E-59 | -1.69E-46 |
| MS.gene054595 | MS.gene58826 | 0.809853 | 1.54E-50 | -1.69E-46 |
| MS.gene054600 | MS.gene58826 | 0.86041 | 2.11E-63 | -1.69E-46 |
| MS.gene054642 | MS.gene58826 | 0.88317 | 5.63E-71 | -1.69E-46 |
| MS.gene05470 | MS.gene58826 | 0.838679 | 2.49E-57 | -1.69E-46 |
| MS.gene054802 | MS.gene58826 | 0.824907 | 6.26E-54 | -1.69E-46 |
| MS.gene054803 | MS.gene58826 | 0.82532 | 5.00E-54 | -1.69E-46 |
| MS.gene054804 | MS.gene58826 | 0.828201 | 1.03E-54 | -1.69E-46 |
| MS.gene054927 | MS.gene58826 | 0.800334 | 1.51E-48 | -1.69E-46 |
| MS.gene054954 | MS.gene58826 | 0.859839 | 3.13E-63 | -1.69E-46 |
| MS.gene05505 | MS.gene58826 | 0.816295 | 5.95E-52 | -1.69E-46 |
| MS.gene055206 | MS.gene58826 | 0.805595 | 1.24E-49 | -1.69E-46 |
| MS.gene055212 | MS.gene58826 | 0.857071 | 2.10E-62 | -1.69E-46 |
| MS.gene055289 | MS.gene58826 | 0.824321 | 8.60E-54 | -1.69E-46 |
| MS.gene055362 | MS.gene58826 | 0.90309 | 4.97E-79 | -1.69E-46 |
| MS.gene055397 | MS.gene58826 | 0.825553 | 4.40E-54 | -1.69E-46 |
| MS.gene055499 | MS.gene58826 | 0.865742 | 4.73E-65 | -1.69E-46 |
| MS.gene055502 | MS.gene58826 | 0.868516 | 6.16E-66 | -1.69E-46 |
| MS.gene055577 | MS.gene58826 | 0.824564 | 7.54E-54 | -1.69E-46 |
| MS.gene055647 | MS.gene58826 | 0.82472 | 6.93E-54 | -1.69E-46 |
| MS.gene055722 | MS.gene58826 | 0.820464 | 6.76E-53 | -1.69E-46 |
| MS.gene055748 | MS.gene58826 | 0.813056 | 3.10E-51 | -1.69E-46 |
| MS.gene055750 | MS.gene58826 | 0.810102 | 1.36E-50 | -1.69E-46 |
| MS.gene055751 | MS.gene58826 | 0.80229 | 6.02E-49 | -1.69E-46 |
| MS.gene05592 | MS.gene58826 | 0.807532 | 4.83E-50 | -1.69E-46 |
| MS.gene055941 | MS.gene58826 | 0.818117 | 2.32E-52 | -1.69E-46 |
| MS.gene055956 | MS.gene58826 | -0.82933 | 5.47E-55 | -1.69E-46 |
| MS.gene056083 | MS.gene58826 | 0.856754 | 2.60E-62 | -1.69E-46 |
| MS.gene056374 | MS.gene58826 | 0.885517 | 7.59E-72 | -1.69E-46 |
| MS.gene056504 | MS.gene58826 | 0.825521 | 4.48E-54 | -1.69E-46 |
| MS.gene056510 | MS.gene58826 | 0.818941 | 1.51E-52 | -1.69E-46 |
| MS.gene056610 | MS.gene58826 | 0.801401 | 9.16E-49 | -1.69E-46 |
| MS.gene056657 | MS.gene58826 | 0.898039 | 7.84E-77 | -1.69E-46 |
| MS.gene056692 | MS.gene58826 | 0.837281 | 5.70E-57 | -1.69E-46 |
| MS.gene057011 | MS.gene58826 | 0.823059 | 1.70E-53 | -1.69E-46 |
| MS.gene057129 | MS.gene58826 | 0.875337 | 3.34E-68 | -1.69E-46 |
| MS.gene057196 | MS.gene58826 | 0.804276 | 2.33E-49 | -1.69E-46 |
| MS.gene057331 | MS.gene58826 | 0.813297 | 2.75E-51 | -1.69E-46 |
| MS.gene057416 | MS.gene58826 | 0.884679 | 1.56E-71 | -1.69E-46 |
| MS.gene057658 | MS.gene58826 | 0.842546 | 2.42E-58 | -1.69E-46 |
| MS.gene057831 | MS.gene58826 | 0.830409 | 2.99E-55 | -1.69E-46 |
| MS.gene058019 | MS.gene58826 | 0.853553 | 2.21E-61 | -1.69E-46 |
| MS.gene058323 | MS.gene58826 | 0.831979 | 1.23E-55 | -1.69E-46 |
| MS.gene058419 | MS.gene58826 | 0.897603 | 1.20E-76 | -1.69E-46 |
| MS.gene058544 | MS.gene58826 | 0.886254 | 4.01E-72 | -1.69E-46 |
| MS.gene058723 | MS.gene58826 | 0.82182 | 3.29E-53 | -1.69E-46 |
| MS.gene058773 | MS.gene58826 | 0.839626 | 1.42E-57 | -1.69E-46 |
| MS.gene058838 | MS.gene58826 | 0.842924 | 1.92E-58 | -1.69E-46 |
| MS.gene059022 | MS.gene58826 | 0.874245 | 7.85E-68 | -1.69E-46 |
| MS.gene059023 | MS.gene58826 | 0.836071 | 1.16E-56 | -1.69E-46 |
| MS.gene05934 | MS.gene58826 | 0.826187 | 3.11E-54 | -1.69E-46 |
| MS.gene059536 | MS.gene58826 | 0.823443 | 1.38E-53 | -1.69E-46 |
| MS.gene059677 | MS.gene58826 | 0.898901 | 3.37E-77 | -1.69E-46 |
| MS.gene059718 | MS.gene58826 | 0.868948 | 4.47E-66 | -1.69E-46 |
| MS.gene059756 | MS.gene58826 | 0.858399 | 8.46E-63 | -1.69E-46 |
| MS.gene059801 | MS.gene58826 | 0.805681 | 1.19E-49 | -1.69E-46 |
| MS.gene059858 | MS.gene58826 | 0.804895 | 1.73E-49 | -1.69E-46 |
| MS.gene059865 | MS.gene58826 | 0.807114 | 5.92E-50 | -1.69E-46 |
| MS.gene059960 | MS.gene58826 | 0.841559 | 4.42E-58 | -1.69E-46 |
| MS.gene060703 | MS.gene58826 | 0.810883 | 9.24E-51 | -1.69E-46 |
| MS.gene060762 | MS.gene58826 | 0.825279 | 5.11E-54 | -1.69E-46 |
| MS.gene06077 | MS.gene58826 | 0.862887 | 3.69E-64 | -1.69E-46 |
| MS.gene060804 | MS.gene58826 | 0.814633 | 1.39E-51 | -1.69E-46 |
| MS.gene06092 | MS.gene58826 | 0.854202 | 1.44E-61 | -1.69E-46 |
| MS.gene061150 | MS.gene58826 | 0.894416 | 2.52E-75 | -1.69E-46 |
| MS.gene061200 | MS.gene58826 | 0.849385 | 3.34E-60 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58826.t1 | MTR_7g088880 | 95.131 | 267 | 5 | 1 | 1 | 267 | 1 | 259 | 0.0 | 509 |
| MS.gene58826.t1 | MTR_3g089890 | 31.707 | 164 | 91 | 6 | 108 | 267 | 121 | 267 | 1.48e-19 | 85.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58826.t1 | AT3G56650 | 74.380 | 242 | 60 | 2 | 28 | 267 | 21 | 262 | 2.92e-129 | 367 |
| MS.gene58826.t1 | AT1G77090 | 31.098 | 164 | 92 | 6 | 108 | 267 | 113 | 259 | 4.80e-19 | 84.3 |
Find 62 sgRNAs with CRISPR-Local
Find 108 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCCATGGCTCTGCACATTT+TGG | 0.188022 | 7.3:+20523181 | None:intergenic |
| CCCTTCAAGAATTCTCTTCT+TGG | 0.220023 | 7.3:+20523527 | None:intergenic |
| AACAGAAAACTAAACTTTAA+AGG | 0.234300 | 7.3:+20522088 | None:intergenic |
| GTGCTTTGGAAGTGAGTTTC+AGG | 0.256242 | 7.3:+20523551 | None:intergenic |
| TCCGAAGCTGTTTCCATGTT+GGG | 0.295637 | 7.3:+20523236 | None:intergenic |
| GAAACTCCATATGCTCTTAC+TGG | 0.304540 | 7.3:-20522227 | MS.gene58826:CDS |
| ATCTGGATCTAAAGTGTTTC+CGG | 0.321003 | 7.3:+20523015 | None:intergenic |
| AATTGTTGCATTTGGTTTGT+TGG | 0.333981 | 7.3:+20523090 | None:intergenic |
| CGAAGGGCAGGAGTATCTTT+AGG | 0.356398 | 7.3:+20523377 | None:intergenic |
| ATTCTCTTCTTGGTGTGCTT+TGG | 0.366749 | 7.3:+20523537 | None:intergenic |
| GAAGCCTCGAGGAGCTCATC+TGG | 0.367527 | 7.3:+20522998 | None:intergenic |
| ATGTCCTCAATTGTTGCATT+TGG | 0.371030 | 7.3:+20523082 | None:intergenic |
| TTGCATTTGGTTTGTTGGTT+AGG | 0.381877 | 7.3:+20523095 | None:intergenic |
| CGGATAGCTAACATACTATC+AGG | 0.410990 | 7.3:-20523217 | MS.gene58826:CDS |
| AGATGAGCTCCTCGAGGCTT+CGG | 0.416414 | 7.3:-20522996 | MS.gene58826:CDS |
| AGTTTGCCACTGCTTGTCAT+TGG | 0.423778 | 7.3:+20522142 | None:intergenic |
| TGTGAACCAGTAAGAGCATA+TGG | 0.439225 | 7.3:+20522221 | None:intergenic |
| ATCCGAAGCTGTTTCCATGT+TGG | 0.463719 | 7.3:+20523235 | None:intergenic |
| AAAGTGTTTCCGGTGACAAA+TGG | 0.467671 | 7.3:+20523025 | None:intergenic |
| ATGCAACAATTGAGGACATC+GGG | 0.486193 | 7.3:-20523078 | MS.gene58826:CDS |
| ATGAAGTTGTGAATGGGAAA+AGG | 0.490618 | 7.3:+20523642 | None:intergenic |
| AATGGGAAAAGGGAGGTGAA+TGG | 0.499776 | 7.3:+20523653 | None:intergenic |
| CCCCAACATGGAAACAGCTT+CGG | 0.500004 | 7.3:-20523237 | MS.gene58826:CDS |
| GTAGTTGATGAAGTTGTGAA+TGG | 0.502381 | 7.3:+20523635 | None:intergenic |
| CTTGCAAAAGCAACAGCAAA+GGG | 0.514105 | 7.3:-20522194 | MS.gene58826:CDS |
| ACCAAAATGTGCAGAGCCAT+GGG | 0.517062 | 7.3:-20523182 | MS.gene58826:CDS |
| AAGGATCTGATGATGAAGGA+GGG | 0.525395 | 7.3:+20523423 | None:intergenic |
| GAAGGATCTGATGATGAAGG+AGG | 0.525960 | 7.3:+20523422 | None:intergenic |
| AACCAAAATGTGCAGAGCCA+TGG | 0.527719 | 7.3:-20523183 | MS.gene58826:CDS |
| CCGAAGCTGTTTCCATGTTG+GGG | 0.529262 | 7.3:+20523237 | None:intergenic |
| GATACTCCTGCCCTTCGTGC+TGG | 0.532133 | 7.3:-20523371 | MS.gene58826:intron |
| CAAACCAAATGCAACAATTG+AGG | 0.532140 | 7.3:-20523086 | MS.gene58826:CDS |
| AAAGAAGGATCTGATGATGA+AGG | 0.536478 | 7.3:+20523419 | None:intergenic |
| AATGTACCAGCACGAAGGGC+AGG | 0.538174 | 7.3:+20523365 | None:intergenic |
| AAATGGTAAGAGCTTTGAAT+GGG | 0.539576 | 7.3:+20523610 | None:intergenic |
| GAAATGGTAAGAGCTTTGAA+TGG | 0.543485 | 7.3:+20523609 | None:intergenic |
| ATGTGCAGAGCCATGGGTCG+AGG | 0.546349 | 7.3:-20523176 | MS.gene58826:CDS |
| ACCAAGAAGAGAATTCTTGA+AGG | 0.556299 | 7.3:-20523528 | MS.gene58826:CDS |
| TGAAGTTGTGAATGGGAAAA+GGG | 0.559321 | 7.3:+20523643 | None:intergenic |
| TCTCTCGGTCCATTTGTCAC+CGG | 0.560247 | 7.3:-20523034 | MS.gene58826:CDS |
| GCTTTGAAGAGAACAAAAGA+AGG | 0.562441 | 7.3:+20523404 | None:intergenic |
| TCAAATTTAACCTCGACCCA+TGG | 0.563391 | 7.3:+20523166 | None:intergenic |
| TCTTGCAAAAGCAACAGCAA+AGG | 0.565478 | 7.3:-20522195 | MS.gene58826:CDS |
| TTGTTGGTTAGGCGTATCAA+AGG | 0.567096 | 7.3:+20523106 | None:intergenic |
| CAAGCTAGAGAAGTTTCAGT+TGG | 0.578431 | 7.3:-20523455 | MS.gene58826:CDS |
| ACTTCTCTAGCTTGTGACTG+TGG | 0.595522 | 7.3:+20523464 | None:intergenic |
| AATCAATGTACCAGCACGAA+GGG | 0.598231 | 7.3:+20523361 | None:intergenic |
| AACTTTAAAGGAATCAAGCA+TGG | 0.601558 | 7.3:+20522100 | None:intergenic |
| CGAAGCTGTTTCCATGTTGG+GGG | 0.605438 | 7.3:+20523238 | None:intergenic |
| TAGTTGATGAAGTTGTGAAT+GGG | 0.614272 | 7.3:+20523636 | None:intergenic |
| AGGAACTGAGACAGAAGCAG+AGG | 0.618479 | 7.3:+20523571 | None:intergenic |
| GAAAAGCTTATCGCTTCTCT+CGG | 0.619390 | 7.3:-20523049 | MS.gene58826:CDS |
| GATCTGATGATGAAGGAGGG+AGG | 0.623035 | 7.3:+20523426 | None:intergenic |
| TCTCTAGCTTGTGACTGTGG+TGG | 0.623327 | 7.3:+20523467 | None:intergenic |
| AATGCAACAATTGAGGACAT+CGG | 0.624867 | 7.3:-20523079 | MS.gene58826:CDS |
| TGAAAAGATTGATGATCAGA+CGG | 0.625680 | 7.3:-20522972 | MS.gene58826:intron |
| CCAAGAAGAGAATTCTTGAA+GGG | 0.629087 | 7.3:-20523527 | MS.gene58826:CDS |
| CTAGTGCCAATGACAAGCAG+TGG | 0.640793 | 7.3:-20522148 | MS.gene58826:CDS |
| CAATCAATGTACCAGCACGA+AGG | 0.662156 | 7.3:+20523360 | None:intergenic |
| GGTTCTGTGAGAGCAATAAG+TGG | 0.687982 | 7.3:+20523488 | None:intergenic |
| AGATCCAGATGAGCTCCTCG+AGG | 0.689017 | 7.3:-20523002 | MS.gene58826:CDS |
| AGTTGTGAATGGGAAAAGGG+AGG | 0.723640 | 7.3:+20523646 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAACTTAGTTCAAATATAA+CGG | - | chr7.3:20523277-20523296 | MS.gene58826:intron | 15.0% |
| !! | AAATTTGAAGATGAAAAACA+AGG | - | chr7.3:20522611-20522630 | MS.gene58826:intron | 20.0% |
| !! | AGTGGTATTATTATAGAAAA+TGG | - | chr7.3:20522913-20522932 | MS.gene58826:intron | 20.0% |
| !! | GTGGTATTATTATAGAAAAT+GGG | - | chr7.3:20522914-20522933 | MS.gene58826:intron | 20.0% |
| !! | TAACTAACATGACTAAAAAA+TGG | - | chr7.3:20523485-20523504 | MS.gene58826:CDS | 20.0% |
| !!! | TATATATCTGAAACTATGAT+AGG | + | chr7.3:20522954-20522973 | None:intergenic | 20.0% |
| ! | ATGTAAATGTTATCTGTTGT+TGG | - | chr7.3:20523452-20523471 | MS.gene58826:CDS | 25.0% |
| ! | TGAAAAACAAGGAAAAATTC+AGG | - | chr7.3:20522622-20522641 | MS.gene58826:intron | 25.0% |
| AACTTGTAAATTAGGAGGAT+TGG | + | chr7.3:20523306-20523325 | None:intergenic | 30.0% | |
| ATAGTCTGATAGAAATCAAG+TGG | - | chr7.3:20522895-20522914 | MS.gene58826:intron | 30.0% | |
| GATTCATATTCGCTAATAAG+TGG | + | chr7.3:20523379-20523398 | None:intergenic | 30.0% | |
| ! | AACTTTAAAGGAATCAAGCA+TGG | + | chr7.3:20523665-20523684 | None:intergenic | 30.0% |
| ! | TCGTTGAATATGATTAGCTA+TGG | - | chr7.3:20523094-20523113 | MS.gene58826:CDS | 30.0% |
| ! | TGAAAAGATTGATGATCAGA+CGG | - | chr7.3:20522790-20522809 | MS.gene58826:intron | 30.0% |
| !! | AAATGGTAAGAGCTTTGAAT+GGG | + | chr7.3:20522155-20522174 | None:intergenic | 30.0% |
| !! | AATTGTTGCATTTGGTTTGT+TGG | + | chr7.3:20522675-20522694 | None:intergenic | 30.0% |
| !! | GTCTATATTTAGTTCTTTGC+AGG | - | chr7.3:20522473-20522492 | MS.gene58826:intron | 30.0% |
| !! | TAGTTGATGAAGTTGTGAAT+GGG | + | chr7.3:20522129-20522148 | None:intergenic | 30.0% |
| !!! | AAACACTGTTGTTTTGTTTG+TGG | - | chr7.3:20523591-20523610 | MS.gene58826:CDS | 30.0% |
| AATGCAACAATTGAGGACAT+CGG | - | chr7.3:20522683-20522702 | MS.gene58826:intron | 35.0% | |
| ACCAAGAAGAGAATTCTTGA+AGG | - | chr7.3:20522234-20522253 | MS.gene58826:CDS | 35.0% | |
| ATGAAGTTGTGAATGGGAAA+AGG | + | chr7.3:20522123-20522142 | None:intergenic | 35.0% | |
| ATGTCCTCAATTGTTGCATT+TGG | + | chr7.3:20522683-20522702 | None:intergenic | 35.0% | |
| CAAACCAAATGCAACAATTG+AGG | - | chr7.3:20522676-20522695 | MS.gene58826:intron | 35.0% | |
| CAACGGTTAATCACGAAATT+CGG | - | chr7.3:20523177-20523196 | MS.gene58826:CDS | 35.0% | |
| CAGGGGTAAACTTGTAAATT+AGG | + | chr7.3:20523314-20523333 | None:intergenic | 35.0% | |
| CCAAAAAAATGATGAGAGCA+GGG | + | chr7.3:20523413-20523432 | None:intergenic | 35.0% | |
| CCAAGAAGAGAATTCTTGAA+GGG | - | chr7.3:20522235-20522254 | MS.gene58826:CDS | 35.0% | |
| GGGTAAACTTGTAAATTAGG+AGG | + | chr7.3:20523311-20523330 | None:intergenic | 35.0% | |
| TGAAGTTGTGAATGGGAAAA+GGG | + | chr7.3:20522122-20522141 | None:intergenic | 35.0% | |
| TTACATTTCAAGCAGCAGTT+TGG | + | chr7.3:20523438-20523457 | None:intergenic | 35.0% | |
| ! | AAAGAAGGATCTGATGATGA+AGG | + | chr7.3:20522346-20522365 | None:intergenic | 35.0% |
| ! | ATCTGGATCTAAAGTGTTTC+CGG | + | chr7.3:20522750-20522769 | None:intergenic | 35.0% |
| ! | GCTTTTATGAATGCACGAAA+TGG | + | chr7.3:20522172-20522191 | None:intergenic | 35.0% |
| !! | ACAAGGAAAAATTCAGGTTG+TGG | - | chr7.3:20522628-20522647 | MS.gene58826:intron | 35.0% |
| !! | GAAATGGTAAGAGCTTTGAA+TGG | + | chr7.3:20522156-20522175 | None:intergenic | 35.0% |
| !! | GCTTTGAAGAGAACAAAAGA+AGG | + | chr7.3:20522361-20522380 | None:intergenic | 35.0% |
| !! | GTAGTTGATGAAGTTGTGAA+TGG | + | chr7.3:20522130-20522149 | None:intergenic | 35.0% |
| !! | TTGCATTTGGTTTGTTGGTT+AGG | + | chr7.3:20522670-20522689 | None:intergenic | 35.0% |
| !!! | CCTGCTCTCATCATTTTTTT+GGG | - | chr7.3:20523411-20523430 | MS.gene58826:CDS | 35.0% |
| AATCAATGTACCAGCACGAA+GGG | + | chr7.3:20522404-20522423 | None:intergenic | 40.0% | |
| AATTCCCTCAGCATAACCTA+TGG | + | chr7.3:20522998-20523017 | None:intergenic | 40.0% | |
| ATGCAACAATTGAGGACATC+GGG | - | chr7.3:20522684-20522703 | MS.gene58826:intron | 40.0% | |
| CAAGCTAGAGAAGTTTCAGT+TGG | - | chr7.3:20522307-20522326 | MS.gene58826:intron | 40.0% | |
| CCCAAAAAAATGATGAGAGC+AGG | + | chr7.3:20523414-20523433 | None:intergenic | 40.0% | |
| CCCTTCAAGAATTCTCTTCT+TGG | + | chr7.3:20522238-20522257 | None:intergenic | 40.0% | |
| CGGATAGCTAACATACTATC+AGG | - | chr7.3:20522545-20522564 | MS.gene58826:intron | 40.0% | |
| CTTGCAAAAGCAACAGCAAA+GGG | - | chr7.3:20523568-20523587 | MS.gene58826:CDS | 40.0% | |
| GAAAAGCTTATCGCTTCTCT+CGG | - | chr7.3:20522713-20522732 | MS.gene58826:intron | 40.0% | |
| GAAACTCCATATGCTCTTAC+TGG | - | chr7.3:20523535-20523554 | MS.gene58826:CDS | 40.0% | |
| GACAAACACTCGAACATAGT+GGG | + | chr7.3:20523357-20523376 | None:intergenic | 40.0% | |
| TACAACAGTCACTCTGTTGT+CGG | + | chr7.3:20523220-20523239 | None:intergenic | 40.0% | |
| TCAAATTTAACCTCGACCCA+TGG | + | chr7.3:20522599-20522618 | None:intergenic | 40.0% | |
| TCTTGCAAAAGCAACAGCAA+AGG | - | chr7.3:20523567-20523586 | MS.gene58826:CDS | 40.0% | |
| ! | AAAGTGTTTCCGGTGACAAA+TGG | + | chr7.3:20522740-20522759 | None:intergenic | 40.0% |
| ! | AAGGATCTGATGATGAAGGA+GGG | + | chr7.3:20522342-20522361 | None:intergenic | 40.0% |
| ! | AGAGAAGCGATAAGCTTTTC+TGG | + | chr7.3:20522713-20522732 | None:intergenic | 40.0% |
| ! | ATAAACGCACAAGAGCACAA+CGG | - | chr7.3:20523160-20523179 | MS.gene58826:CDS | 40.0% |
| ! | ATTCTCTTCTTGGTGTGCTT+TGG | + | chr7.3:20522228-20522247 | None:intergenic | 40.0% |
| ! | GAGAAGCGATAAGCTTTTCT+GGG | + | chr7.3:20522712-20522731 | None:intergenic | 40.0% |
| ! | TGTGAACCAGTAAGAGCATA+TGG | + | chr7.3:20523544-20523563 | None:intergenic | 40.0% |
| ! | TTGTTGGTTAGGCGTATCAA+AGG | + | chr7.3:20522659-20522678 | None:intergenic | 40.0% |
| !!! | CCCTGCTCTCATCATTTTTT+TGG | - | chr7.3:20523410-20523429 | MS.gene58826:CDS | 40.0% |
| AACCAAAATGTGCAGAGCCA+TGG | - | chr7.3:20522579-20522598 | MS.gene58826:intron | 45.0% | |
| AATGGGAAAAGGGAGGTGAA+TGG | + | chr7.3:20522112-20522131 | None:intergenic | 45.0% | |
| ACCAAAATGTGCAGAGCCAT+GGG | - | chr7.3:20522580-20522599 | MS.gene58826:intron | 45.0% | |
| AGTTGTGAATGGGAAAAGGG+AGG | + | chr7.3:20522119-20522138 | None:intergenic | 45.0% | |
| AGTTTGCCACTGCTTGTCAT+TGG | + | chr7.3:20523623-20523642 | None:intergenic | 45.0% | |
| CAATCAATGTACCAGCACGA+AGG | + | chr7.3:20522405-20522424 | None:intergenic | 45.0% | |
| CCTACCATAGGTTATGCTGA+GGG | - | chr7.3:20522991-20523010 | MS.gene58826:CDS | 45.0% | |
| GAGTTTCATCCTCCTACCAT+AGG | - | chr7.3:20522979-20522998 | MS.gene58826:CDS | 45.0% | |
| GGACAAACACTCGAACATAG+TGG | + | chr7.3:20523358-20523377 | None:intergenic | 45.0% | |
| GGTTCTGTGAGAGCAATAAG+TGG | + | chr7.3:20522277-20522296 | None:intergenic | 45.0% | |
| TCAGCATAACCTATGGTAGG+AGG | + | chr7.3:20522991-20523010 | None:intergenic | 45.0% | |
| TCCTACCATAGGTTATGCTG+AGG | - | chr7.3:20522990-20523009 | MS.gene58826:CDS | 45.0% | |
| ! | ACTTCTCTAGCTTGTGACTG+TGG | + | chr7.3:20522301-20522320 | None:intergenic | 45.0% |
| ! | ATCCGAAGCTGTTTCCATGT+TGG | + | chr7.3:20522530-20522549 | None:intergenic | 45.0% |
| ! | GAAGGATCTGATGATGAAGG+AGG | + | chr7.3:20522343-20522362 | None:intergenic | 45.0% |
| ! | TCCGAAGCTGTTTCCATGTT+GGG | + | chr7.3:20522529-20522548 | None:intergenic | 45.0% |
| !! | GTGCTTTGGAAGTGAGTTTC+AGG | + | chr7.3:20522214-20522233 | None:intergenic | 45.0% |
| AACCCCTGACGTACAGTTCA+GGG | + | chr7.3:20523332-20523351 | None:intergenic | 50.0% | |
| AGGAACTGAGACAGAAGCAG+AGG | + | chr7.3:20522194-20522213 | None:intergenic | 50.0% | |
| AGTTCATCCTTCCCCCAACA+TGG | - | chr7.3:20522513-20522532 | MS.gene58826:intron | 50.0% | |
| ATGTTGGGGGAAGGATGAAC+TGG | + | chr7.3:20522514-20522533 | None:intergenic | 50.0% | |
| CCCCAACATGGAAACAGCTT+CGG | - | chr7.3:20522525-20522544 | MS.gene58826:intron | 50.0% | |
| CCCTCAGCATAACCTATGGT+AGG | + | chr7.3:20522994-20523013 | None:intergenic | 50.0% | |
| CTAGTGCCAATGACAAGCAG+TGG | - | chr7.3:20523614-20523633 | MS.gene58826:CDS | 50.0% | |
| TACCCCTGAACTGTACGTCA+GGG | - | chr7.3:20523326-20523345 | MS.gene58826:intron | 50.0% | |
| TCTCTCGGTCCATTTGTCAC+CGG | - | chr7.3:20522728-20522747 | MS.gene58826:intron | 50.0% | |
| TTACCCCTGAACTGTACGTC+AGG | - | chr7.3:20523325-20523344 | MS.gene58826:intron | 50.0% | |
| ! | CCGAAGCTGTTTCCATGTTG+GGG | + | chr7.3:20522528-20522547 | None:intergenic | 50.0% |
| ! | CGAAGCTGTTTCCATGTTGG+GGG | + | chr7.3:20522527-20522546 | None:intergenic | 50.0% |
| ! | GATCTGATGATGAAGGAGGG+AGG | + | chr7.3:20522339-20522358 | None:intergenic | 50.0% |
| ! | TCTCTAGCTTGTGACTGTGG+TGG | + | chr7.3:20522298-20522317 | None:intergenic | 50.0% |
| ! | TCTTTTCAACCGAAGCCTCG+AGG | + | chr7.3:20522778-20522797 | None:intergenic | 50.0% |
| !! | ACCCATGGCTCTGCACATTT+TGG | + | chr7.3:20522584-20522603 | None:intergenic | 50.0% |
| !! | CGAAGGGCAGGAGTATCTTT+AGG | + | chr7.3:20522388-20522407 | None:intergenic | 50.0% |
| AATGTACCAGCACGAAGGGC+AGG | + | chr7.3:20522400-20522419 | None:intergenic | 55.0% | |
| ACCCCTGAACTGTACGTCAG+GGG | - | chr7.3:20523327-20523346 | MS.gene58826:intron | 55.0% | |
| ACCCCTGACGTACAGTTCAG+GGG | + | chr7.3:20523331-20523350 | None:intergenic | 55.0% | |
| AGATCCAGATGAGCTCCTCG+AGG | - | chr7.3:20522760-20522779 | MS.gene58826:intron | 55.0% | |
| AGATGAGCTCCTCGAGGCTT+CGG | - | chr7.3:20522766-20522785 | MS.gene58826:intron | 55.0% | |
| GAACCCCTGACGTACAGTTC+AGG | + | chr7.3:20523333-20523352 | None:intergenic | 55.0% | |
| GGGGGAAGGATGAACTGGTA+TGG | + | chr7.3:20522509-20522528 | None:intergenic | 55.0% | |
| ! | GCTGTTTCCATGTTGGGGGA+AGG | + | chr7.3:20522523-20522542 | None:intergenic | 55.0% |
| ATGTGCAGAGCCATGGGTCG+AGG | - | chr7.3:20522586-20522605 | MS.gene58826:intron | 60.0% | |
| GAAGCCTCGAGGAGCTCATC+TGG | + | chr7.3:20522767-20522786 | None:intergenic | 60.0% | |
| GATACTCCTGCCCTTCGTGC+TGG | - | chr7.3:20522391-20522410 | MS.gene58826:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 20522097 | 20523687 | 20522097 | ID=MS.gene58826 |
| chr7.3 | mRNA | 20522097 | 20523687 | 20522097 | ID=MS.gene58826.t1;Parent=MS.gene58826 |
| chr7.3 | exon | 20523372 | 20523687 | 20523372 | ID=MS.gene58826.t1.exon1;Parent=MS.gene58826.t1 |
| chr7.3 | CDS | 20523372 | 20523687 | 20523372 | ID=cds.MS.gene58826.t1;Parent=MS.gene58826.t1 |
| chr7.3 | exon | 20522973 | 20523289 | 20522973 | ID=MS.gene58826.t1.exon2;Parent=MS.gene58826.t1 |
| chr7.3 | CDS | 20522973 | 20523289 | 20522973 | ID=cds.MS.gene58826.t1;Parent=MS.gene58826.t1 |
| chr7.3 | exon | 20522097 | 20522267 | 20522097 | ID=MS.gene58826.t1.exon3;Parent=MS.gene58826.t1 |
| chr7.3 | CDS | 20522097 | 20522267 | 20522097 | ID=cds.MS.gene58826.t1;Parent=MS.gene58826.t1 |
| Gene Sequence |
| Protein sequence |
