Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82112.t1 | XP_013452660.1 | 95.7 | 211 | 8 | 1 | 1 | 210 | 1 | 211 | 2.00E-100 | 375.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82112.t1 | Q9SJ52 | 44.1 | 161 | 84 | 5 | 26 | 184 | 60 | 216 | 3.7e-26 | 119.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82112.t1 | A0A072UCB3 | 95.7 | 211 | 8 | 1 | 1 | 210 | 1 | 211 | 1.5e-100 | 375.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene82112 | -0.833804 | 4.32E-56 | -1.69E-46 |
| MS.gene049217 | MS.gene82112 | -0.802735 | 4.87E-49 | -1.69E-46 |
| MS.gene049222 | MS.gene82112 | -0.806874 | 6.66E-50 | -1.69E-46 |
| MS.gene049259 | MS.gene82112 | 0.818836 | 1.59E-52 | -1.69E-46 |
| MS.gene049281 | MS.gene82112 | 0.854869 | 9.23E-62 | -1.69E-46 |
| MS.gene049285 | MS.gene82112 | -0.821826 | 3.28E-53 | -1.69E-46 |
| MS.gene049320 | MS.gene82112 | -0.846652 | 1.90E-59 | -1.69E-46 |
| MS.gene049549 | MS.gene82112 | 0.802763 | 4.81E-49 | -1.69E-46 |
| MS.gene049611 | MS.gene82112 | 0.826464 | 2.67E-54 | -1.69E-46 |
| MS.gene049633 | MS.gene82112 | -0.810564 | 1.08E-50 | -1.69E-46 |
| MS.gene049634 | MS.gene82112 | 0.843965 | 1.01E-58 | -1.69E-46 |
| MS.gene050095 | MS.gene82112 | 0.820282 | 7.45E-53 | -1.69E-46 |
| MS.gene050096 | MS.gene82112 | 0.811169 | 8.01E-51 | -1.69E-46 |
| MS.gene05010 | MS.gene82112 | -0.803369 | 3.60E-49 | -1.69E-46 |
| MS.gene050163 | MS.gene82112 | -0.807642 | 4.57E-50 | -1.69E-46 |
| MS.gene050165 | MS.gene82112 | -0.810283 | 1.24E-50 | -1.69E-46 |
| MS.gene050170 | MS.gene82112 | -0.800228 | 1.59E-48 | -1.69E-46 |
| MS.gene050363 | MS.gene82112 | 0.82189 | 3.17E-53 | -1.69E-46 |
| MS.gene050381 | MS.gene82112 | 0.865986 | 3.96E-65 | -1.69E-46 |
| MS.gene050496 | MS.gene82112 | -0.81582 | 7.60E-52 | -1.69E-46 |
| MS.gene050504 | MS.gene82112 | -0.846924 | 1.60E-59 | -1.69E-46 |
| MS.gene050577 | MS.gene82112 | -0.800559 | 1.36E-48 | -1.69E-46 |
| MS.gene050612 | MS.gene82112 | -0.849062 | 4.12E-60 | -1.69E-46 |
| MS.gene050675 | MS.gene82112 | -0.826742 | 2.30E-54 | -1.69E-46 |
| MS.gene050699 | MS.gene82112 | -0.826388 | 2.79E-54 | -1.69E-46 |
| MS.gene050772 | MS.gene82112 | -0.800393 | 1.47E-48 | -1.69E-46 |
| MS.gene05079 | MS.gene82112 | 0.802037 | 6.78E-49 | -1.69E-46 |
| MS.gene05080 | MS.gene82112 | -0.814242 | 1.70E-51 | -1.69E-46 |
| MS.gene050868 | MS.gene82112 | -0.846479 | 2.12E-59 | -1.69E-46 |
| MS.gene050871 | MS.gene82112 | -0.842509 | 2.48E-58 | -1.69E-46 |
| MS.gene050883 | MS.gene82112 | 0.849887 | 2.42E-60 | -1.69E-46 |
| MS.gene050986 | MS.gene82112 | -0.824147 | 9.45E-54 | -1.69E-46 |
| MS.gene051044 | MS.gene82112 | 0.86824 | 7.56E-66 | -1.69E-46 |
| MS.gene05104 | MS.gene82112 | 0.819013 | 1.45E-52 | -1.69E-46 |
| MS.gene051127 | MS.gene82112 | 0.869526 | 2.90E-66 | -1.69E-46 |
| MS.gene051138 | MS.gene82112 | 0.881199 | 2.93E-70 | -1.69E-46 |
| MS.gene051364 | MS.gene82112 | 0.820205 | 7.76E-53 | -1.69E-46 |
| MS.gene051366 | MS.gene82112 | 0.810246 | 1.27E-50 | -1.69E-46 |
| MS.gene05141 | MS.gene82112 | 0.811426 | 7.04E-51 | -1.69E-46 |
| MS.gene051422 | MS.gene82112 | 0.802282 | 6.04E-49 | -1.69E-46 |
| MS.gene051632 | MS.gene82112 | 0.810423 | 1.16E-50 | -1.69E-46 |
| MS.gene051651 | MS.gene82112 | 0.807123 | 5.89E-50 | -1.69E-46 |
| MS.gene051810 | MS.gene82112 | -0.81218 | 4.83E-51 | -1.69E-46 |
| MS.gene051946 | MS.gene82112 | 0.849005 | 4.27E-60 | -1.69E-46 |
| MS.gene051973 | MS.gene82112 | 0.824844 | 6.48E-54 | -1.69E-46 |
| MS.gene052164 | MS.gene82112 | -0.815203 | 1.04E-51 | -1.69E-46 |
| MS.gene052211 | MS.gene82112 | 0.822372 | 2.45E-53 | -1.69E-46 |
| MS.gene052482 | MS.gene82112 | -0.808948 | 2.41E-50 | -1.69E-46 |
| MS.gene052486 | MS.gene82112 | -0.816149 | 6.42E-52 | -1.69E-46 |
| MS.gene052802 | MS.gene82112 | 0.80241 | 5.69E-49 | -1.69E-46 |
| MS.gene052966 | MS.gene82112 | -0.802434 | 5.62E-49 | -1.69E-46 |
| MS.gene053203 | MS.gene82112 | -0.817602 | 3.03E-52 | -1.69E-46 |
| MS.gene053420 | MS.gene82112 | 0.874229 | 7.95E-68 | -1.69E-46 |
| MS.gene053488 | MS.gene82112 | 0.870582 | 1.31E-66 | -1.69E-46 |
| MS.gene053549 | MS.gene82112 | 0.831051 | 2.08E-55 | -1.69E-46 |
| MS.gene053561 | MS.gene82112 | 0.867628 | 1.19E-65 | -1.69E-46 |
| MS.gene053887 | MS.gene82112 | 0.845241 | 4.59E-59 | -1.69E-46 |
| MS.gene054054 | MS.gene82112 | 0.846254 | 2.44E-59 | -1.69E-46 |
| MS.gene054056 | MS.gene82112 | 0.831144 | 1.97E-55 | -1.69E-46 |
| MS.gene054616 | MS.gene82112 | 0.804218 | 2.40E-49 | -1.69E-46 |
| MS.gene054642 | MS.gene82112 | -0.815034 | 1.14E-51 | -1.69E-46 |
| MS.gene054706 | MS.gene82112 | 0.808544 | 2.94E-50 | -1.69E-46 |
| MS.gene054708 | MS.gene82112 | 0.846667 | 1.88E-59 | -1.69E-46 |
| MS.gene05470 | MS.gene82112 | -0.831644 | 1.49E-55 | -1.69E-46 |
| MS.gene054954 | MS.gene82112 | -0.818589 | 1.81E-52 | -1.69E-46 |
| MS.gene054959 | MS.gene82112 | 0.861032 | 1.36E-63 | -1.69E-46 |
| MS.gene055124 | MS.gene82112 | 0.841309 | 5.14E-58 | -1.69E-46 |
| MS.gene055154 | MS.gene82112 | 0.873193 | 1.78E-67 | -1.69E-46 |
| MS.gene055202 | MS.gene82112 | 0.818569 | 1.83E-52 | -1.69E-46 |
| MS.gene055240 | MS.gene82112 | 0.803649 | 3.15E-49 | -1.69E-46 |
| MS.gene055362 | MS.gene82112 | -0.841689 | 4.08E-58 | -1.69E-46 |
| MS.gene055502 | MS.gene82112 | -0.850008 | 2.24E-60 | -1.69E-46 |
| MS.gene055577 | MS.gene82112 | -0.837695 | 4.47E-57 | -1.69E-46 |
| MS.gene055748 | MS.gene82112 | -0.801176 | 1.02E-48 | -1.69E-46 |
| MS.gene055751 | MS.gene82112 | -0.806955 | 6.40E-50 | -1.69E-46 |
| MS.gene05589 | MS.gene82112 | -0.837801 | 4.20E-57 | -1.69E-46 |
| MS.gene055942 | MS.gene82112 | 0.847073 | 1.46E-59 | -1.69E-46 |
| MS.gene056224 | MS.gene82112 | 0.848233 | 6.98E-60 | -1.69E-46 |
| MS.gene056310 | MS.gene82112 | 0.811681 | 6.20E-51 | -1.69E-46 |
| MS.gene056311 | MS.gene82112 | 0.83158 | 1.54E-55 | -1.69E-46 |
| MS.gene056313 | MS.gene82112 | 0.814492 | 1.50E-51 | -1.69E-46 |
| MS.gene056504 | MS.gene82112 | -0.837768 | 4.28E-57 | -1.69E-46 |
| MS.gene056510 | MS.gene82112 | -0.800371 | 1.49E-48 | -1.69E-46 |
| MS.gene056550 | MS.gene82112 | 0.811406 | 7.11E-51 | -1.69E-46 |
| MS.gene056657 | MS.gene82112 | -0.821627 | 3.65E-53 | -1.69E-46 |
| MS.gene056692 | MS.gene82112 | -0.807594 | 4.68E-50 | -1.69E-46 |
| MS.gene056748 | MS.gene82112 | 0.813985 | 1.94E-51 | -1.69E-46 |
| MS.gene056761 | MS.gene82112 | 0.802821 | 4.68E-49 | -1.69E-46 |
| MS.gene056856 | MS.gene82112 | 0.831441 | 1.67E-55 | -1.69E-46 |
| MS.gene056857 | MS.gene82112 | 0.814386 | 1.58E-51 | -1.69E-46 |
| MS.gene056988 | MS.gene82112 | 0.827533 | 1.48E-54 | -1.69E-46 |
| MS.gene057014 | MS.gene82112 | 0.853644 | 2.08E-61 | -1.69E-46 |
| MS.gene057261 | MS.gene82112 | 0.80125 | 9.84E-49 | -1.69E-46 |
| MS.gene057548 | MS.gene82112 | 0.81565 | 8.29E-52 | -1.69E-46 |
| MS.gene057549 | MS.gene82112 | 0.808308 | 3.30E-50 | -1.69E-46 |
| MS.gene058068 | MS.gene82112 | 0.854093 | 1.55E-61 | -1.69E-46 |
| MS.gene058099 | MS.gene82112 | 0.84868 | 5.25E-60 | -1.69E-46 |
| MS.gene058109 | MS.gene82112 | 0.812819 | 3.50E-51 | -1.69E-46 |
| MS.gene058111 | MS.gene82112 | 0.802674 | 5.02E-49 | -1.69E-46 |
| MS.gene058117 | MS.gene82112 | 0.827009 | 1.98E-54 | -1.69E-46 |
| MS.gene058171 | MS.gene82112 | 0.827844 | 1.25E-54 | -1.69E-46 |
| MS.gene058173 | MS.gene82112 | 0.813787 | 2.14E-51 | -1.69E-46 |
| MS.gene058175 | MS.gene82112 | 0.828818 | 7.28E-55 | -1.69E-46 |
| MS.gene058268 | MS.gene82112 | 0.824666 | 7.13E-54 | -1.69E-46 |
| MS.gene058269 | MS.gene82112 | 0.826395 | 2.78E-54 | -1.69E-46 |
| MS.gene058334 | MS.gene82112 | 0.808681 | 2.75E-50 | -1.69E-46 |
| MS.gene058341 | MS.gene82112 | 0.812682 | 3.75E-51 | -1.69E-46 |
| MS.gene05837 | MS.gene82112 | -0.836029 | 1.19E-56 | -1.69E-46 |
| MS.gene058419 | MS.gene82112 | -0.827621 | 1.41E-54 | -1.69E-46 |
| MS.gene058493 | MS.gene82112 | 0.836071 | 1.16E-56 | -1.69E-46 |
| MS.gene058494 | MS.gene82112 | 0.816456 | 5.48E-52 | -1.69E-46 |
| MS.gene058544 | MS.gene82112 | -0.821607 | 3.69E-53 | -1.69E-46 |
| MS.gene058603 | MS.gene82112 | 0.874608 | 5.92E-68 | -1.69E-46 |
| MS.gene058628 | MS.gene82112 | 0.808441 | 3.09E-50 | -1.69E-46 |
| MS.gene058630 | MS.gene82112 | 0.804683 | 1.92E-49 | -1.69E-46 |
| MS.gene058773 | MS.gene82112 | -0.822418 | 2.39E-53 | -1.69E-46 |
| MS.gene058838 | MS.gene82112 | -0.807392 | 5.17E-50 | -1.69E-46 |
| MS.gene058840 | MS.gene82112 | 0.805789 | 1.13E-49 | -1.69E-46 |
| MS.gene059022 | MS.gene82112 | -0.85474 | 1.01E-61 | -1.69E-46 |
| MS.gene059023 | MS.gene82112 | -0.823329 | 1.47E-53 | -1.69E-46 |
| MS.gene059223 | MS.gene82112 | 0.827348 | 1.64E-54 | -1.69E-46 |
| MS.gene059677 | MS.gene82112 | -0.837035 | 6.59E-57 | -1.69E-46 |
| MS.gene059690 | MS.gene82112 | 0.847706 | 9.76E-60 | -1.69E-46 |
| MS.gene059691 | MS.gene82112 | 0.848006 | 8.07E-60 | -1.69E-46 |
| MS.gene059694 | MS.gene82112 | 0.860837 | 1.56E-63 | -1.69E-46 |
| MS.gene059697 | MS.gene82112 | 0.860561 | 1.90E-63 | -1.69E-46 |
| MS.gene059718 | MS.gene82112 | -0.828609 | 8.17E-55 | -1.69E-46 |
| MS.gene059865 | MS.gene82112 | -0.837004 | 6.71E-57 | -1.69E-46 |
| MS.gene059902 | MS.gene82112 | -0.802958 | 4.38E-49 | -1.69E-46 |
| MS.gene059916 | MS.gene82112 | 0.843365 | 1.47E-58 | -1.69E-46 |
| MS.gene059953 | MS.gene82112 | 0.820614 | 6.25E-53 | -1.69E-46 |
| MS.gene059955 | MS.gene82112 | 0.804124 | 2.51E-49 | -1.69E-46 |
| MS.gene060595 | MS.gene82112 | 0.80209 | 6.62E-49 | -1.69E-46 |
| MS.gene060762 | MS.gene82112 | -0.834473 | 2.94E-56 | -1.69E-46 |
| MS.gene060778 | MS.gene82112 | -0.802889 | 4.53E-49 | -1.69E-46 |
| MS.gene06077 | MS.gene82112 | -0.841264 | 5.28E-58 | -1.69E-46 |
| MS.gene060982 | MS.gene82112 | -0.819648 | 1.04E-52 | -1.69E-46 |
| MS.gene061044 | MS.gene82112 | 0.821441 | 4.03E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82112.t1 | MTR_6g471080 | 93.365 | 211 | 13 | 1 | 1 | 210 | 1 | 211 | 5.64e-129 | 362 |
| MS.gene82112.t1 | MTR_6g471090 | 80.976 | 205 | 38 | 1 | 3 | 206 | 6 | 210 | 7.14e-115 | 326 |
| MS.gene82112.t1 | MTR_6g071410 | 74.057 | 212 | 49 | 2 | 3 | 208 | 7 | 218 | 9.12e-105 | 301 |
| MS.gene82112.t1 | MTR_6g070890 | 74.057 | 212 | 49 | 2 | 3 | 208 | 7 | 218 | 9.12e-105 | 301 |
| MS.gene82112.t1 | MTR_6g070900 | 70.192 | 208 | 60 | 2 | 3 | 208 | 7 | 214 | 1.70e-96 | 280 |
| MS.gene82112.t1 | MTR_6g071400 | 70.192 | 208 | 60 | 2 | 3 | 208 | 7 | 214 | 1.70e-96 | 280 |
| MS.gene82112.t1 | MTR_6g071710 | 69.737 | 152 | 45 | 1 | 58 | 208 | 11 | 162 | 5.13e-66 | 201 |
| MS.gene82112.t1 | MTR_2g048270 | 58.553 | 152 | 55 | 5 | 45 | 195 | 2 | 146 | 1.57e-56 | 177 |
| MS.gene82112.t1 | MTR_1g057270 | 42.529 | 174 | 89 | 8 | 24 | 194 | 74 | 239 | 1.53e-32 | 118 |
| MS.gene82112.t1 | MTR_4g119428 | 41.111 | 180 | 97 | 7 | 17 | 194 | 65 | 237 | 6.66e-32 | 116 |
| MS.gene82112.t1 | MTR_7g106030 | 33.659 | 205 | 111 | 8 | 6 | 194 | 33 | 228 | 1.04e-29 | 110 |
| MS.gene82112.t1 | MTR_7g106010 | 34.759 | 187 | 112 | 6 | 11 | 193 | 43 | 223 | 4.93e-29 | 108 |
| MS.gene82112.t1 | MTR_7g105870 | 44.231 | 156 | 80 | 6 | 21 | 174 | 57 | 207 | 6.81e-26 | 100 |
| MS.gene82112.t1 | MTR_7g106000 | 32.474 | 194 | 108 | 9 | 5 | 183 | 31 | 216 | 4.20e-24 | 95.5 |
| MS.gene82112.t1 | MTR_7g105970 | 31.707 | 205 | 111 | 10 | 5 | 193 | 31 | 222 | 6.13e-24 | 95.1 |
| MS.gene82112.t1 | MTR_1g057400 | 30.288 | 208 | 116 | 10 | 5 | 194 | 10 | 206 | 2.78e-21 | 87.8 |
| MS.gene82112.t1 | MTR_6g453010 | 35.465 | 172 | 85 | 5 | 11 | 176 | 17 | 168 | 1.97e-19 | 82.4 |
| MS.gene82112.t1 | MTR_2g011350 | 34.884 | 86 | 55 | 1 | 20 | 104 | 37 | 122 | 2.23e-14 | 69.3 |
| MS.gene82112.t1 | MTR_7g106050 | 28.947 | 152 | 101 | 4 | 6 | 154 | 19 | 166 | 4.99e-14 | 68.6 |
| MS.gene82112.t1 | MTR_3g063310 | 34.074 | 135 | 76 | 5 | 21 | 148 | 83 | 211 | 1.25e-13 | 68.2 |
| MS.gene82112.t1 | MTR_1g057290 | 34.503 | 171 | 97 | 8 | 30 | 193 | 51 | 213 | 2.58e-12 | 63.9 |
| MS.gene82112.t1 | MTR_2g087000 | 25.568 | 176 | 118 | 4 | 9 | 180 | 52 | 218 | 3.73e-12 | 63.5 |
| MS.gene82112.t1 | MTR_1g019920 | 42.105 | 76 | 38 | 3 | 19 | 89 | 3 | 77 | 4.47e-12 | 60.1 |
| MS.gene82112.t1 | MTR_2g086790 | 25.146 | 171 | 111 | 5 | 4 | 161 | 16 | 182 | 7.58e-12 | 62.8 |
| MS.gene82112.t1 | MTR_5g076170 | 32.283 | 127 | 83 | 3 | 24 | 148 | 97 | 222 | 1.21e-11 | 62.4 |
| MS.gene82112.t1 | MTR_1g057420 | 31.884 | 138 | 88 | 5 | 26 | 161 | 39 | 172 | 6.45e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82112.t1 | AT2G35980 | 44.318 | 176 | 81 | 6 | 12 | 174 | 35 | 206 | 1.78e-36 | 127 |
| MS.gene82112.t1 | AT5G06320 | 39.247 | 186 | 102 | 8 | 11 | 194 | 52 | 228 | 7.71e-32 | 115 |
| MS.gene82112.t1 | AT2G35460 | 35.450 | 189 | 112 | 7 | 7 | 193 | 54 | 234 | 5.71e-31 | 114 |
| MS.gene82112.t1 | AT3G11650 | 32.984 | 191 | 120 | 6 | 4 | 193 | 52 | 235 | 9.74e-30 | 110 |
| MS.gene82112.t1 | AT3G52470 | 33.636 | 110 | 71 | 2 | 4 | 111 | 16 | 125 | 4.73e-16 | 73.9 |
| MS.gene82112.t1 | AT1G32270 | 30.323 | 155 | 101 | 5 | 28 | 180 | 1 | 150 | 1.20e-15 | 74.7 |
| MS.gene82112.t1 | AT1G32270 | 30.323 | 155 | 101 | 5 | 28 | 180 | 1 | 150 | 2.66e-15 | 73.9 |
| MS.gene82112.t1 | AT3G11660 | 30.539 | 167 | 98 | 6 | 4 | 161 | 15 | 172 | 1.00e-14 | 70.5 |
| MS.gene82112.t1 | AT4G09590 | 28.477 | 151 | 103 | 5 | 6 | 154 | 21 | 168 | 1.20e-13 | 67.4 |
| MS.gene82112.t1 | AT2G35960 | 31.469 | 143 | 92 | 5 | 21 | 161 | 35 | 173 | 6.65e-13 | 65.5 |
| MS.gene82112.t1 | AT2G35970 | 28.477 | 151 | 103 | 5 | 6 | 154 | 21 | 168 | 2.62e-12 | 63.9 |
| MS.gene82112.t1 | AT1G65690 | 30.872 | 149 | 96 | 6 | 9 | 154 | 71 | 215 | 1.02e-11 | 62.8 |
| MS.gene82112.t1 | AT5G22870 | 25.000 | 188 | 119 | 6 | 10 | 184 | 24 | 202 | 1.15e-11 | 62.0 |
| MS.gene82112.t1 | AT3G44220 | 29.932 | 147 | 95 | 6 | 16 | 160 | 31 | 171 | 3.42e-11 | 60.5 |
| MS.gene82112.t1 | AT5G06330 | 31.333 | 150 | 92 | 7 | 26 | 170 | 39 | 182 | 8.71e-11 | 59.7 |
Find 40 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCACCACTGCTACATCTTC+TGG | 0.249772 | 6.1:-63769483 | None:intergenic |
| CTTCTGGGACTAAAAGAAAA+TGG | 0.303320 | 6.1:-63769467 | None:intergenic |
| TAATTACCATCATCGTCTTC+TGG | 0.307893 | 6.1:+63768970 | MS.gene82112:CDS |
| TCTTGGGATTTATAAAGATT+TGG | 0.312711 | 6.1:+63769310 | MS.gene82112:CDS |
| TAGCAGTGGTGATTTCTTTA+CGG | 0.343521 | 6.1:+63769493 | MS.gene82112:CDS |
| ATTCGAATTAGAGCTAAGTA+TGG | 0.426821 | 6.1:+63769347 | MS.gene82112:CDS |
| TCGTCGTTTGAGAGTTCCTT+TGG | 0.428461 | 6.1:+63769415 | MS.gene82112:CDS |
| AAACGACGACACTGCAGAAC+CGG | 0.436027 | 6.1:-63769401 | None:intergenic |
| ACATGAAATTTCACGCTTGA+GGG | 0.443845 | 6.1:-63769005 | None:intergenic |
| GACTGGTCGGTTTAATCCAC+CGG | 0.447589 | 6.1:+63769382 | MS.gene82112:CDS |
| ATAGACTATGATGTTGTTGT+TGG | 0.461075 | 6.1:-63769111 | None:intergenic |
| TCACCACTGCTACATCTTCT+GGG | 0.464477 | 6.1:-63769482 | None:intergenic |
| TAGACTATGATGTTGTTGTT+GGG | 0.464761 | 6.1:-63769110 | None:intergenic |
| ATTCATCACCAATATGGGTT+GGG | 0.469338 | 6.1:+63768902 | None:intergenic |
| ACTTTGACACCCTTTGATCA+AGG | 0.484433 | 6.1:+63769188 | MS.gene82112:CDS |
| TGGTGATGATGAGTTACTAT+TGG | 0.487019 | 6.1:-63769447 | None:intergenic |
| TACATGAAATTTCACGCTTG+AGG | 0.491389 | 6.1:-63769006 | None:intergenic |
| TATTCTGAAGAGACACGTCT+TGG | 0.496905 | 6.1:+63769293 | MS.gene82112:CDS |
| GGAAATTCTATCATCAAACT+TGG | 0.500531 | 6.1:+63769251 | MS.gene82112:CDS |
| CTTGGACCTATCGAATTCAA+AGG | 0.500883 | 6.1:+63769230 | MS.gene82112:CDS |
| TTGCTGCTTTATTTATATCA+CGG | 0.504913 | 6.1:-63769517 | None:intergenic |
| AATTCATCACCAATATGGGT+TGG | 0.511959 | 6.1:+63768901 | None:intergenic |
| TATGGAAGCTTCTATAAGAC+TGG | 0.528774 | 6.1:+63769365 | MS.gene82112:CDS |
| ATTCTGAAGAGACACGTCTT+GGG | 0.539671 | 6.1:+63769294 | MS.gene82112:CDS |
| AGTCTATTATCGAAGAATCA+AGG | 0.541807 | 6.1:+63769127 | MS.gene82112:CDS |
| AGACTATGATGTTGTTGTTG+GGG | 0.552465 | 6.1:-63769109 | None:intergenic |
| TTCGATAGGTCCAAGAAAAG+TGG | 0.557190 | 6.1:-63769222 | None:intergenic |
| GAAGAATCAAGGCCATTGCT+TGG | 0.570010 | 6.1:+63769138 | MS.gene82112:CDS |
| AATATAAGTTGTTGTTTGTG+AGG | 0.572559 | 6.1:-63769055 | None:intergenic |
| GAATTTCCTTTGAATTCGAT+AGG | 0.573832 | 6.1:-63769236 | None:intergenic |
| ATGAAATTTCACGCTTGAGG+GGG | 0.578027 | 6.1:-63769003 | None:intergenic |
| ATTGTCTTTGTACCAAGCAA+TGG | 0.580644 | 6.1:-63769150 | None:intergenic |
| GAAGCTTCTATAAGACTGGT+CGG | 0.586174 | 6.1:+63769369 | MS.gene82112:CDS |
| TATTATCCAGAAGACGATGA+TGG | 0.595014 | 6.1:-63768976 | None:intergenic |
| GTTGTTTGTGAGGTTGAACT+CGG | 0.599773 | 6.1:-63769045 | None:intergenic |
| CATGAAATTTCACGCTTGAG+GGG | 0.610657 | 6.1:-63769004 | None:intergenic |
| CGACGACACTGCAGAACCGG+TGG | 0.610886 | 6.1:-63769398 | None:intergenic |
| CGATGATGGTAATTACGAAG+AGG | 0.647200 | 6.1:-63768962 | None:intergenic |
| AGTCCCAGAAGATGTAGCAG+TGG | 0.649919 | 6.1:+63769479 | MS.gene82112:CDS |
| CTATTGGAAGAAGAAACCAA+AGG | 0.773456 | 6.1:-63769431 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TCTTGGGATTTATAAAGATT+TGG | + | chr6.1:63769310-63769329 | MS.gene82112:CDS | 25.0% |
| !!! | AATATAAGTTGTTGTTTGTG+AGG | - | chr6.1:63769058-63769077 | None:intergenic | 25.0% |
| !!! | TTGCTGCTTTATTTATATCA+CGG | - | chr6.1:63769520-63769539 | None:intergenic | 25.0% |
| AGTCTATTATCGAAGAATCA+AGG | + | chr6.1:63769127-63769146 | MS.gene82112:CDS | 30.0% | |
| ATAGACTATGATGTTGTTGT+TGG | - | chr6.1:63769114-63769133 | None:intergenic | 30.0% | |
| ATTCGAATTAGAGCTAAGTA+TGG | + | chr6.1:63769347-63769366 | MS.gene82112:CDS | 30.0% | |
| GAATTTCCTTTGAATTCGAT+AGG | - | chr6.1:63769239-63769258 | None:intergenic | 30.0% | |
| GGAAATTCTATCATCAAACT+TGG | + | chr6.1:63769251-63769270 | MS.gene82112:CDS | 30.0% | |
| ! | ATTTTTGTGTCCTTGATCAA+AGG | - | chr6.1:63769201-63769220 | None:intergenic | 30.0% |
| ! | TAGACTATGATGTTGTTGTT+GGG | - | chr6.1:63769113-63769132 | None:intergenic | 30.0% |
| ! | TTTTTGTGTCCTTGATCAAA+GGG | - | chr6.1:63769200-63769219 | None:intergenic | 30.0% |
| !! | CACAAAAATACCACTTTTCT+TGG | + | chr6.1:63769212-63769231 | MS.gene82112:CDS | 30.0% |
| ACATGAAATTTCACGCTTGA+GGG | - | chr6.1:63769008-63769027 | None:intergenic | 35.0% | |
| ATTGTCTTTGTACCAAGCAA+TGG | - | chr6.1:63769153-63769172 | None:intergenic | 35.0% | |
| CTATTGGAAGAAGAAACCAA+AGG | - | chr6.1:63769434-63769453 | None:intergenic | 35.0% | |
| TAATTACCATCATCGTCTTC+TGG | + | chr6.1:63768970-63768989 | MS.gene82112:CDS | 35.0% | |
| TACATGAAATTTCACGCTTG+AGG | - | chr6.1:63769009-63769028 | None:intergenic | 35.0% | |
| TAGCAGTGGTGATTTCTTTA+CGG | + | chr6.1:63769493-63769512 | MS.gene82112:CDS | 35.0% | |
| TATGGAAGCTTCTATAAGAC+TGG | + | chr6.1:63769365-63769384 | MS.gene82112:CDS | 35.0% | |
| TATTATCCAGAAGACGATGA+TGG | - | chr6.1:63768979-63768998 | None:intergenic | 35.0% | |
| ! | AGACTATGATGTTGTTGTTG+GGG | - | chr6.1:63769112-63769131 | None:intergenic | 35.0% |
| ! | TGGTGATGATGAGTTACTAT+TGG | - | chr6.1:63769450-63769469 | None:intergenic | 35.0% |
| !! | CTTCTGGGACTAAAAGAAAA+TGG | - | chr6.1:63769470-63769489 | None:intergenic | 35.0% |
| ACTTTGACACCCTTTGATCA+AGG | + | chr6.1:63769188-63769207 | MS.gene82112:CDS | 40.0% | |
| ATGAAATTTCACGCTTGAGG+GGG | - | chr6.1:63769006-63769025 | None:intergenic | 40.0% | |
| CATGAAATTTCACGCTTGAG+GGG | - | chr6.1:63769007-63769026 | None:intergenic | 40.0% | |
| CTTGGACCTATCGAATTCAA+AGG | + | chr6.1:63769230-63769249 | MS.gene82112:CDS | 40.0% | |
| GAAGCTTCTATAAGACTGGT+CGG | + | chr6.1:63769369-63769388 | MS.gene82112:CDS | 40.0% | |
| GTTGTTTGTGAGGTTGAACT+CGG | - | chr6.1:63769048-63769067 | None:intergenic | 40.0% | |
| TTCGATAGGTCCAAGAAAAG+TGG | - | chr6.1:63769225-63769244 | None:intergenic | 40.0% | |
| ! | ATTCTGAAGAGACACGTCTT+GGG | + | chr6.1:63769294-63769313 | MS.gene82112:CDS | 40.0% |
| ! | CGATGATGGTAATTACGAAG+AGG | - | chr6.1:63768965-63768984 | None:intergenic | 40.0% |
| ! | TATTCTGAAGAGACACGTCT+TGG | + | chr6.1:63769293-63769312 | MS.gene82112:CDS | 40.0% |
| ATCACCACTGCTACATCTTC+TGG | - | chr6.1:63769486-63769505 | None:intergenic | 45.0% | |
| GAAGAATCAAGGCCATTGCT+TGG | + | chr6.1:63769138-63769157 | MS.gene82112:CDS | 45.0% | |
| TCACCACTGCTACATCTTCT+GGG | - | chr6.1:63769485-63769504 | None:intergenic | 45.0% | |
| TCGTCGTTTGAGAGTTCCTT+TGG | + | chr6.1:63769415-63769434 | MS.gene82112:CDS | 45.0% | |
| AAACGACGACACTGCAGAAC+CGG | - | chr6.1:63769404-63769423 | None:intergenic | 50.0% | |
| AGTCCCAGAAGATGTAGCAG+TGG | + | chr6.1:63769479-63769498 | MS.gene82112:CDS | 50.0% | |
| ! | GACTGGTCGGTTTAATCCAC+CGG | + | chr6.1:63769382-63769401 | MS.gene82112:CDS | 50.0% |
| CGACGACACTGCAGAACCGG+TGG | - | chr6.1:63769401-63769420 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 63768915 | 63769547 | 63768915 | ID=MS.gene82112 |
| chr6.1 | mRNA | 63768915 | 63769547 | 63768915 | ID=MS.gene82112.t1;Parent=MS.gene82112 |
| chr6.1 | exon | 63768915 | 63769547 | 63768915 | ID=MS.gene82112.t1.exon1;Parent=MS.gene82112.t1 |
| chr6.1 | CDS | 63768915 | 63769547 | 63768915 | ID=cds.MS.gene82112.t1;Parent=MS.gene82112.t1 |
| Gene Sequence |
| Protein sequence |
