AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene97395


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene97395.t1 MTR_3g015440 90.909 143 12 1 12 154 1 142 5.84e-92 267
MS.gene97395.t1 MTR_3g015440 91.549 142 11 1 13 154 2 142 8.29e-92 267
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene97395.t1 AT3G17670 48.855 131 57 2 33 154 6 135 3.30e-35 122
MS.gene97395.t1 AT3G17670 48.855 131 57 2 33 154 6 135 4.48e-35 123

Find 62 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGCACACCCGTATTTCTT+TGG 0.222264 3.1:+10102506 None:intergenic
CTGCTGCTGCTGTGCTTTGG+TGG 0.284352 3.1:+10102533 None:intergenic
CGGGACGGCTGCTCGTTGTT+TGG 0.315956 3.1:-10102325 MS.gene97395:CDS
GGAAGGAAGCCAGCTTTGTT+TGG 0.321861 3.1:+10102560 None:intergenic
CTGCTGTGCTTTGGTGGTGC+AGG 0.322544 3.1:+10102539 None:intergenic
CTTCTGCTGCTGCTGTGCTT+TGG 0.338547 3.1:+10102530 None:intergenic
GTGCACACCCGTATTTCTTT+GGG 0.344936 3.1:+10102507 None:intergenic
GCCAGCTTTGTTTGGGTGAT+GGG 0.345679 3.1:+10102568 None:intergenic
TGTAAAGTCGTCCGGTTGCT+TGG 0.362043 3.1:-10102409 MS.gene97395:CDS
ACCTCATCATCCGCTATTGC+GGG 0.397200 3.1:-10102344 MS.gene97395:CDS
GAAGGAAGCCAGCTTTGTTT+GGG 0.402768 3.1:+10102561 None:intergenic
GAGACCATGGTAAGTCTGTT+TGG 0.410408 3.1:-10102303 MS.gene97395:CDS
GACGACTTTACAGAGACGTT+TGG 0.431405 3.1:+10102420 None:intergenic
TGCTCTTGCTTTGAGGAAGA+AGG 0.433872 3.1:-10102259 MS.gene97395:CDS
AATTGGCGTGGATGCGGTTA+CGG 0.442355 3.1:+10102632 None:intergenic
TGGTGGAGGAGATAATAGTT+TGG 0.444099 3.1:-10102283 MS.gene97395:CDS
GAGAGTTTCTAGAGTTTGAA+AGG 0.447053 3.1:+10102458 None:intergenic
TGCTTGGGTAAGTGCGGTGC+TGG 0.449594 3.1:-10102393 MS.gene97395:CDS
TAGCGGATGATGAGGTAATC+AGG 0.450567 3.1:+10102351 None:intergenic
AAGAGACTGTGGGATTGAAT+TGG 0.451131 3.1:+10102615 None:intergenic
GTAAAGTCGTCCGGTTGCTT+GGG 0.451387 3.1:-10102408 MS.gene97395:CDS
AGCAGAAGCCCAAAGAAATA+CGG 0.454994 3.1:-10102515 MS.gene97395:CDS
GTTTGGATGCTCTTGCTTTG+AGG 0.461598 3.1:-10102266 MS.gene97395:CDS
AGCCAGCTTTGTTTGGGTGA+TGG 0.465425 3.1:+10102567 None:intergenic
ACCGCACTTACCCAAGCAAC+CGG 0.465811 3.1:+10102398 None:intergenic
CTCTTTGTAAGCTGTCATGT+GGG 0.472330 3.1:-10102597 MS.gene97395:CDS
TCAGGAAGGACCACCAGGTT+GGG 0.478230 3.1:+10102369 None:intergenic
AAAGGATCCTTGTTTGCGGC+AGG 0.482679 3.1:+10102476 None:intergenic
TTGTTTGCGGCAGGCACGGT+TGG 0.493358 3.1:+10102485 None:intergenic
ATCCTTGTTTGCGGCAGGCA+CGG 0.497473 3.1:+10102481 None:intergenic
GACTTTACAGAGACGTTTGG+AGG 0.498021 3.1:+10102423 None:intergenic
AACGTCTCTGTAAAGTCGTC+CGG 0.511697 3.1:-10102417 MS.gene97395:CDS
CCAGCTTTGTTTGGGTGATG+GGG 0.513955 3.1:+10102569 None:intergenic
GGTAATCAGGAAGGACCACC+AGG 0.515194 3.1:+10102364 None:intergenic
CCCCATCACCCAAACAAAGC+TGG 0.526664 3.1:-10102569 MS.gene97395:CDS
TACCTCATCATCCGCTATTG+CGG 0.534019 3.1:-10102345 MS.gene97395:CDS
AACCGTGCCTGCCGCAAACA+AGG 0.534436 3.1:-10102483 MS.gene97395:CDS
CAAACTCTAGAAACTCTCTC+TGG 0.537664 3.1:-10102453 MS.gene97395:CDS
GTGCGGTGCTGGTCCCAACC+TGG 0.538727 3.1:-10102382 MS.gene97395:CDS
TGGCGTGGATGCGGTTACGG+TGG 0.540320 3.1:+10102635 None:intergenic
TTTGAAAGGATCCTTGTTTG+CGG 0.546105 3.1:+10102472 None:intergenic
TGCTCGTTGTTTGGAGACCA+TGG 0.558612 3.1:-10102316 MS.gene97395:CDS
GGCGTGGATGCGGTTACGGT+GGG 0.560111 3.1:+10102636 None:intergenic
GCAGAAGCCCAAAGAAATAC+GGG 0.565624 3.1:-10102514 MS.gene97395:CDS
ATCAGGAAGGACCACCAGGT+TGG 0.575050 3.1:+10102368 None:intergenic
GGATTGAATTGGCGTGGATG+CGG 0.576414 3.1:+10102626 None:intergenic
GAGCAGCCGTCCCGCAATAG+CGG 0.581813 3.1:+10102334 None:intergenic
ATGGTAAGTCTGTTTGGTGG+AGG 0.589868 3.1:-10102297 MS.gene97395:CDS
TGTGCTTTGGTGGTGCAGGA+AGG 0.595048 3.1:+10102543 None:intergenic
ACCATGGTAAGTCTGTTTGG+TGG 0.604006 3.1:-10102300 MS.gene97395:CDS
TCTCTTTGTAAGCTGTCATG+TGG 0.607011 3.1:-10102598 MS.gene97395:CDS
GACAGCTTACAAAGAGACTG+TGG 0.611286 3.1:+10102604 None:intergenic
TCCCGCAATAGCGGATGATG+AGG 0.621652 3.1:+10102343 None:intergenic
TCCGGTTGCTTGGGTAAGTG+CGG 0.626454 3.1:-10102399 MS.gene97395:CDS
TACCTGCGAGAGCAAAAGCT+CGG 0.642802 3.1:+10102200 None:intergenic
GGATGATGAGGTAATCAGGA+AGG 0.643225 3.1:+10102355 None:intergenic
TCCACCAAACAGACTTACCA+TGG 0.651181 3.1:+10102299 None:intergenic
CATCATCCGCTATTGCGGGA+CGG 0.668576 3.1:-10102340 MS.gene97395:CDS
ACAGCTTACAAAGAGACTGT+GGG 0.668910 3.1:+10102605 None:intergenic
TCTTTGTAAGCTGTCATGTG+GGG 0.668932 3.1:-10102596 MS.gene97395:CDS
ACTGTGGGATTGAATTGGCG+TGG 0.696617 3.1:+10102620 None:intergenic
CGGTGCTGGTCCCAACCTGG+TGG 0.700612 3.1:-10102379 MS.gene97395:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AGAGAAGAAAAATTTCGCTG+AGG - chr3.1:10102604-10102623 MS.gene97395:CDS 35.0%
! TTTGAAAGGATCCTTGTTTG+CGG + chr3.1:10102358-10102377 None:intergenic 35.0%
!! GAGAGTTTCTAGAGTTTGAA+AGG + chr3.1:10102372-10102391 None:intergenic 35.0%
AAGAGACTGTGGGATTGAAT+TGG + chr3.1:10102215-10102234 None:intergenic 40.0%
ACAGCTTACAAAGAGACTGT+GGG + chr3.1:10102225-10102244 None:intergenic 40.0%
AGCAGAAGCCCAAAGAAATA+CGG - chr3.1:10102312-10102331 MS.gene97395:CDS 40.0%
CTCTTTGTAAGCTGTCATGT+GGG - chr3.1:10102230-10102249 MS.gene97395:CDS 40.0%
TCTCTTTGTAAGCTGTCATG+TGG - chr3.1:10102229-10102248 MS.gene97395:CDS 40.0%
TCTTTGTAAGCTGTCATGTG+GGG - chr3.1:10102231-10102250 MS.gene97395:CDS 40.0%
TGGTGGAGGAGATAATAGTT+TGG - chr3.1:10102544-10102563 MS.gene97395:CDS 40.0%
! CAAACTCTAGAAACTCTCTC+TGG - chr3.1:10102374-10102393 MS.gene97395:CDS 40.0%
AACGTCTCTGTAAAGTCGTC+CGG - chr3.1:10102410-10102429 MS.gene97395:CDS 45.0%
ACCATGGTAAGTCTGTTTGG+TGG - chr3.1:10102527-10102546 MS.gene97395:CDS 45.0%
GACAGCTTACAAAGAGACTG+TGG + chr3.1:10102226-10102245 None:intergenic 45.0%
GACGACTTTACAGAGACGTT+TGG + chr3.1:10102410-10102429 None:intergenic 45.0%
GACTTTACAGAGACGTTTGG+AGG + chr3.1:10102407-10102426 None:intergenic 45.0%
GAGACCATGGTAAGTCTGTT+TGG - chr3.1:10102524-10102543 MS.gene97395:CDS 45.0%
GCAGAAGCCCAAAGAAATAC+GGG - chr3.1:10102313-10102332 MS.gene97395:CDS 45.0%
GGATGATGAGGTAATCAGGA+AGG + chr3.1:10102475-10102494 None:intergenic 45.0%
GTGCACACCCGTATTTCTTT+GGG + chr3.1:10102323-10102342 None:intergenic 45.0%
GTTTGGATGCTCTTGCTTTG+AGG - chr3.1:10102561-10102580 MS.gene97395:CDS 45.0%
TACCTCATCATCCGCTATTG+CGG - chr3.1:10102482-10102501 MS.gene97395:CDS 45.0%
TCCACCAAACAGACTTACCA+TGG + chr3.1:10102531-10102550 None:intergenic 45.0%
! ATGGTAAGTCTGTTTGGTGG+AGG - chr3.1:10102530-10102549 MS.gene97395:CDS 45.0%
! TAGCGGATGATGAGGTAATC+AGG + chr3.1:10102479-10102498 None:intergenic 45.0%
!! GAAGGAAGCCAGCTTTGTTT+GGG + chr3.1:10102269-10102288 None:intergenic 45.0%
!! TGCTCTTGCTTTGAGGAAGA+AGG - chr3.1:10102568-10102587 MS.gene97395:CDS 45.0%
AATTGGCGTGGATGCGGTTA+CGG + chr3.1:10102198-10102217 None:intergenic 50.0%
ACCTCATCATCCGCTATTGC+GGG - chr3.1:10102483-10102502 MS.gene97395:CDS 50.0%
ACTGTGGGATTGAATTGGCG+TGG + chr3.1:10102210-10102229 None:intergenic 50.0%
GGATTGAATTGGCGTGGATG+CGG + chr3.1:10102204-10102223 None:intergenic 50.0%
GGTGCACACCCGTATTTCTT+TGG + chr3.1:10102324-10102343 None:intergenic 50.0%
GTAAAGTCGTCCGGTTGCTT+GGG - chr3.1:10102419-10102438 MS.gene97395:CDS 50.0%
TACCTGCGAGAGCAAAAGCT+CGG + chr3.1:10102630-10102649 None:intergenic 50.0%
TGCTCGTTGTTTGGAGACCA+TGG - chr3.1:10102511-10102530 MS.gene97395:CDS 50.0%
TGTAAAGTCGTCCGGTTGCT+TGG - chr3.1:10102418-10102437 MS.gene97395:CDS 50.0%
! AAAGGATCCTTGTTTGCGGC+AGG + chr3.1:10102354-10102373 None:intergenic 50.0%
!! AGCCAGCTTTGTTTGGGTGA+TGG + chr3.1:10102263-10102282 None:intergenic 50.0%
!! CCAGCTTTGTTTGGGTGATG+GGG + chr3.1:10102261-10102280 None:intergenic 50.0%
!! GCCAGCTTTGTTTGGGTGAT+GGG + chr3.1:10102262-10102281 None:intergenic 50.0%
!! GGAAGGAAGCCAGCTTTGTT+TGG + chr3.1:10102270-10102289 None:intergenic 50.0%
ACCGCACTTACCCAAGCAAC+CGG + chr3.1:10102432-10102451 None:intergenic 55.0%
ATCAGGAAGGACCACCAGGT+TGG + chr3.1:10102462-10102481 None:intergenic 55.0%
CATCATCCGCTATTGCGGGA+CGG - chr3.1:10102487-10102506 MS.gene97395:CDS 55.0%
CCCCATCACCCAAACAAAGC+TGG - chr3.1:10102258-10102277 MS.gene97395:CDS 55.0%
CTTCTGCTGCTGCTGTGCTT+TGG + chr3.1:10102300-10102319 None:intergenic 55.0%
GGTAATCAGGAAGGACCACC+AGG + chr3.1:10102466-10102485 None:intergenic 55.0%
TCAGGAAGGACCACCAGGTT+GGG + chr3.1:10102461-10102480 None:intergenic 55.0%
TCCGGTTGCTTGGGTAAGTG+CGG - chr3.1:10102428-10102447 MS.gene97395:CDS 55.0%
! ATCCTTGTTTGCGGCAGGCA+CGG + chr3.1:10102349-10102368 None:intergenic 55.0%
!! TCCCGCAATAGCGGATGATG+AGG + chr3.1:10102487-10102506 None:intergenic 55.0%
!! TGTGCTTTGGTGGTGCAGGA+AGG + chr3.1:10102287-10102306 None:intergenic 55.0%
AACCGTGCCTGCCGCAAACA+AGG - chr3.1:10102344-10102363 MS.gene97395:CDS 60.0%
CTGCTGCTGCTGTGCTTTGG+TGG + chr3.1:10102297-10102316 None:intergenic 60.0%
TGCTTGGGTAAGTGCGGTGC+TGG - chr3.1:10102434-10102453 MS.gene97395:CDS 60.0%
TTGTTTGCGGCAGGCACGGT+TGG + chr3.1:10102345-10102364 None:intergenic 60.0%
!! CTGCTGTGCTTTGGTGGTGC+AGG + chr3.1:10102291-10102310 None:intergenic 60.0%
CGGGACGGCTGCTCGTTGTT+TGG - chr3.1:10102502-10102521 MS.gene97395:CDS 65.0%
GAGCAGCCGTCCCGCAATAG+CGG + chr3.1:10102496-10102515 None:intergenic 65.0%
GGCGTGGATGCGGTTACGGT+GGG + chr3.1:10102194-10102213 None:intergenic 65.0%
TGGCGTGGATGCGGTTACGG+TGG + chr3.1:10102195-10102214 None:intergenic 65.0%
! GGCCGAGCTTTTGCTCTCGC+AGG - chr3.1:10102625-10102644 MS.gene97395:CDS 65.0%
!! CGGTGCTGGTCCCAACCTGG+TGG - chr3.1:10102448-10102467 MS.gene97395:CDS 70.0%
!! GTGCGGTGCTGGTCCCAACC+TGG - chr3.1:10102445-10102464 MS.gene97395:CDS 70.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 10102185 10102664 10102185 ID=MS.gene97395
chr3.1 mRNA 10102185 10102664 10102185 ID=MS.gene97395.t1;Parent=MS.gene97395
chr3.1 exon 10102185 10102664 10102185 ID=MS.gene97395.t1.exon1;Parent=MS.gene97395.t1
chr3.1 CDS 10102185 10102664 10102185 ID=cds.MS.gene97395.t1;Parent=MS.gene97395.t1
Gene Sequence

>MS.gene97395

ATGAATCCCACCGTAACCGCATCCACGCCAATTCAATCCCACAGTCTCTTTGTAAGCTGTCATGTGGGGACATCCCCATCACCCAAACAAAGCTGGCTTCCTTCCTGCACCACCAAAGCACAGCAGCAGCAGAAGCCCAAAGAAATACGGGTGTGCACCAACCGTGCCTGCCGCAAACAAGGATCCTTTCAAACTCTAGAAACTCTCTCTGGTATCGCTCCTCCAAACGTCTCTGTAAAGTCGTCCGGTTGCTTGGGTAAGTGCGGTGCTGGTCCCAACCTGGTGGTCCTTCCTGATTACCTCATCATCCGCTATTGCGGGACGGCTGCTCGTTGTTTGGAGACCATGGTAAGTCTGTTTGGTGGAGGAGATAATAGTTTGGATGCTCTTGCTTTGAGGAAGAAGGCTGATGTTGAGTTAGAGAAGAAAAATTTCGCTGAGGCCGAGCTTTTGCTCTCGCAGGTACACATATACTTGTAA

Protein sequence

>MS.gene97395.t1

MNPTVTASTPIQSHSLFVSCHVGTSPSPKQSWLPSCTTKAQQQQKPKEIRVCTNRACRKQGSFQTLETLSGIAPPNVSVKSSGCLGKCGAGPNLVVLPDYLIIRYCGTAARCLETMVSLFGGGDNSLDALALRKKADVELEKKNFAEAELLLSQVHIYL