AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580025899.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025899.01.T01 MTR_5g036560 94.505 91 5 0 3 93 410 500 5.93e-41 140
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 16 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGGAAATTATTTATGAAT+AGG 0.301527 5:-26365595 None:intergenic
ACTTCTTATCGTGGTCAAAA+AGG 0.318891 5:-26364387 MsG0580025899.01.T01:CDS
GACCACGATAAGAAGTTGTT+AGG 0.334966 5:+26364394 None:intergenic
AAAGCTAAAGCATGATTTCA+AGG 0.446740 5:-26364424 MsG0580025899.01.T01:CDS
TCCATCCTCTCAACGGTAGC+AGG 0.474386 5:+26365572 None:intergenic
ACCACGATAAGAAGTTGTTA+GGG 0.483102 5:+26364395 None:intergenic
TGGAAGTGTTGTACGAACTT+TGG 0.489754 5:-26365540 MsG0580025899.01.T01:CDS
GCCTGCTACCGTTGAGAGGA+TGG 0.498871 5:-26365573 MsG0580025899.01.T01:CDS
AGTGACATTAAATCAGAGAA+AGG 0.512134 5:-26365509 MsG0580025899.01.T01:CDS
GCGTCTCCAAACAAAATTGA+AGG 0.515742 5:-26365335 MsG0580025899.01.T01:intron
GTGACATTAAATCAGAGAAA+GGG 0.517883 5:-26365508 MsG0580025899.01.T01:CDS
ATAAGAAGTTGTTAGGGGTG+TGG 0.577233 5:+26364401 None:intergenic
TAAATCAGAGAAAGGGAAAG+CGG 0.634535 5:-26365501 MsG0580025899.01.T01:intron
ATAGGCCTGCTACCGTTGAG+AGG 0.639643 5:-26365577 MsG0580025899.01.T01:CDS
CCCCTAACAACTTCTTATCG+TGG 0.648364 5:-26364396 MsG0580025899.01.T01:CDS
CCACGATAAGAAGTTGTTAG+GGG 0.668169 5:+26364396 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAAAACTTTTTGTAATGTT+TGG - Chr5:26364893-26364912 MsG0580025899.01.T01:intron 15.0%
!!! TAAAACTTTTTGTAATGTTT+GGG - Chr5:26364894-26364913 MsG0580025899.01.T01:intron 15.0%
!!! TGATTTTTTTAAAATCGATT+TGG - Chr5:26364806-26364825 MsG0580025899.01.T01:intron 15.0%
!! CTATAAACTAAATACATGAT+TGG + Chr5:26365380-26365399 None:intergenic 20.0%
!! GATGAAAGAAAAAAATAGTT+TGG + Chr5:26364509-26364528 None:intergenic 20.0%
!!! CATGTATTTAGTTTATAGAA+GGG - Chr5:26365382-26365401 MsG0580025899.01.T01:CDS 20.0%
!!! TCATGTATTTAGTTTATAGA+AGG - Chr5:26365381-26365400 MsG0580025899.01.T01:CDS 20.0%
! AATGATACTTATTGTGTGTT+TGG - Chr5:26364748-26364767 MsG0580025899.01.T01:intron 25.0%
! AGAAGAAAACTACAAATAAG+GGG + Chr5:26364484-26364503 None:intergenic 25.0%
! ATGATACTTATTGTGTGTTT+GGG - Chr5:26364749-26364768 MsG0580025899.01.T01:intron 25.0%
! CAGAAGAAAACTACAAATAA+GGG + Chr5:26364485-26364504 None:intergenic 25.0%
! TCAGAAGAAAACTACAAATA+AGG + Chr5:26364486-26364505 None:intergenic 25.0%
! TCATTTCAAGAGAAAAATCA+TGG + Chr5:26364627-26364646 None:intergenic 25.0%
!! TAATGTTTGGGTTTGATTTA+GGG - Chr5:26364906-26364925 MsG0580025899.01.T01:intron 25.0%
!!! AAACTACTTTTCATCACTTT+TGG - Chr5:26365241-26365260 MsG0580025899.01.T01:intron 25.0%
!!! TCTGATTTAATGTCACTTTT+CGG + Chr5:26364410-26364429 None:intergenic 25.0%
!!! TTTTTCCTCTATTTTTCTTG+AGG + Chr5:26364541-26364560 None:intergenic 25.0%
AAAGCTAAAGCATGATTTCA+AGG - Chr5:26365498-26365517 MsG0580025899.01.T01:intron 30.0%
AGTGACATTAAATCAGAGAA+AGG - Chr5:26364413-26364432 MsG0580025899.01.T01:CDS 30.0%
ATTGAACCATAAAGTGTGTT+TGG + Chr5:26365168-26365187 None:intergenic 30.0%
CAAAATCCAAACACACTTTA+TGG - Chr5:26365159-26365178 MsG0580025899.01.T01:intron 30.0%
GAAGAAAACTACAAATAAGG+GGG + Chr5:26364483-26364502 None:intergenic 30.0%
GGAAAAACCTAATACAAAAG+TGG - Chr5:26364554-26364573 MsG0580025899.01.T01:intron 30.0%
GTGACATTAAATCAGAGAAA+GGG - Chr5:26364414-26364433 MsG0580025899.01.T01:CDS 30.0%
TAGTTTATAGAAGGGATGAT+GGG - Chr5:26365390-26365409 MsG0580025899.01.T01:intron 30.0%
TGACTAACATATCTCAACAT+AGG - Chr5:26365453-26365472 MsG0580025899.01.T01:intron 30.0%
TGATACTTATTGTGTGTTTG+GGG - Chr5:26364750-26364769 MsG0580025899.01.T01:intron 30.0%
TTAGTTTATAGAAGGGATGA+TGG - Chr5:26365389-26365408 MsG0580025899.01.T01:intron 30.0%
TTTCGTAAAGAACGAATGTT+TGG + Chr5:26365310-26365329 None:intergenic 30.0%
! GTAATGTTTGGGTTTGATTT+AGG - Chr5:26364905-26364924 MsG0580025899.01.T01:intron 30.0%
!! AGAGTACCTTCAATTTTGTT+TGG + Chr5:26364596-26364615 None:intergenic 30.0%
!! CTTTCTACCACTTTTGTATT+AGG + Chr5:26364564-26364583 None:intergenic 30.0%
!! GCTATTTTGAAAGATAGGTA+GGG - Chr5:26364716-26364735 MsG0580025899.01.T01:intron 30.0%
!! TTTCTGGCTTTGTAAATTCA+TGG + Chr5:26365097-26365116 None:intergenic 30.0%
!!! CTGATTTAATGTCACTTTTC+GGG + Chr5:26364409-26364428 None:intergenic 30.0%
!!! GAGATGCTATTTTGAAAGAT+AGG - Chr5:26364711-26364730 MsG0580025899.01.T01:intron 30.0%
!!! TGATTTAATGTCACTTTTCG+GGG + Chr5:26364408-26364427 None:intergenic 30.0%
!!! TGCTATTTTGAAAGATAGGT+AGG - Chr5:26364715-26364734 MsG0580025899.01.T01:intron 30.0%
AATAGATTCTAACGCGAAGT+TGG - Chr5:26365010-26365029 MsG0580025899.01.T01:intron 35.0%
ACCACGATAAGAAGTTGTTA+GGG + Chr5:26365530-26365549 None:intergenic 35.0%
ACTTCTTATCGTGGTCAAAA+AGG - Chr5:26365535-26365554 MsG0580025899.01.T01:CDS 35.0%
ATAGATTCTAACGCGAAGTT+GGG - Chr5:26365011-26365030 MsG0580025899.01.T01:intron 35.0%
CTAACATATCTCAACATAGG+AGG - Chr5:26365456-26365475 MsG0580025899.01.T01:intron 35.0%
GAATTTACAAAGCCAGAAAC+TGG - Chr5:26365098-26365117 MsG0580025899.01.T01:intron 35.0%
GATACTTATTGTGTGTTTGG+GGG - Chr5:26364751-26364770 MsG0580025899.01.T01:intron 35.0%
GGAGACATAAAAGTGATTGT+AGG - Chr5:26364927-26364946 MsG0580025899.01.T01:intron 35.0%
TAAATCAGAGAAAGGGAAAG+CGG - Chr5:26364421-26364440 MsG0580025899.01.T01:CDS 35.0%
TCAGTCCTCAAGAAAAATAG+AGG - Chr5:26364533-26364552 MsG0580025899.01.T01:intron 35.0%
TTAGTGCATTTATCTCTCAG+TGG + Chr5:26365211-26365230 None:intergenic 35.0%
! AAAAGTGATTGTAGGAGCAT+AGG - Chr5:26364935-26364954 MsG0580025899.01.T01:intron 35.0%
! CTTCGCGTTAGAATCTATTT+TGG + Chr5:26365009-26365028 None:intergenic 35.0%
! TTAGAGCTTTTGCTTAACAG+TGG - Chr5:26365036-26365055 MsG0580025899.01.T01:intron 35.0%
!! ATACATGATTGGTTTCACAG+TGG + Chr5:26365369-26365388 None:intergenic 35.0%
!! CATCACTTTTGGTTGAATTG+AGG - Chr5:26365252-26365271 MsG0580025899.01.T01:intron 35.0%
!! CTTTTGGTTGAATTGAGGTT+TGG - Chr5:26365257-26365276 MsG0580025899.01.T01:intron 35.0%
!! TAGGAGCATAGGATTGATTT+TGG - Chr5:26364946-26364965 MsG0580025899.01.T01:intron 35.0%
!!! TTTTTTCTCCATCCTCTCAA+CGG + Chr5:26364360-26364379 None:intergenic 35.0%
ATAAGAAGTTGTTAGGGGTG+TGG + Chr5:26365524-26365543 None:intergenic 40.0%
ATCAAAAAGTCGCGCAGAAA+AGG - Chr5:26365558-26365577 MsG0580025899.01.T01:CDS 40.0%
CATTTAAGTGAAACCAACGC+AGG + Chr5:26364667-26364686 None:intergenic 40.0%
CATTTCCTATATTCCTGCGT+TGG - Chr5:26364651-26364670 MsG0580025899.01.T01:intron 40.0%
CCACGATAAGAAGTTGTTAG+GGG + Chr5:26365529-26365548 None:intergenic 40.0%
CTCAACATAGGAGGAAAAAG+AGG - Chr5:26365465-26365484 MsG0580025899.01.T01:intron 40.0%
GACCACGATAAGAAGTTGTT+AGG + Chr5:26365531-26365550 None:intergenic 40.0%
GAGAGGATGGAGAAAAAAAG+TGG - Chr5:26364362-26364381 MsG0580025899.01.T01:CDS 40.0%
GCGTCTCCAAACAAAATTGA+AGG - Chr5:26364587-26364606 MsG0580025899.01.T01:intron 40.0%
TGAAACCAACGCAGGAATAT+AGG + Chr5:26364659-26364678 None:intergenic 40.0%
!! TGGAAGTGTTGTACGAACTT+TGG - Chr5:26364382-26364401 MsG0580025899.01.T01:CDS 40.0%
CCCCTAACAACTTCTTATCG+TGG - Chr5:26365526-26365545 MsG0580025899.01.T01:CDS 45.0%
!! AGGGATGATGGGATATGAGT+TGG - Chr5:26365401-26365420 MsG0580025899.01.T01:intron 45.0%
TCAGAGAAAGGGAAAGCGGT+AGG - Chr5:26364425-26364444 MsG0580025899.01.T01:CDS 50.0%
ATAGGCCTGCTACCGTTGAG+AGG - Chr5:26364345-26364364 MsG0580025899.01.T01:CDS 55.0%
GAGCGAGAAGCTCCAGTTTC+TGG + Chr5:26365113-26365132 None:intergenic 55.0%
TCCATCCTCTCAACGGTAGC+AGG + Chr5:26364353-26364372 None:intergenic 55.0%
GCCTGCTACCGTTGAGAGGA+TGG - Chr5:26364349-26364368 MsG0580025899.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 26364341 26365603 26364341 ID=MsG0580025899.01;Name=MsG0580025899.01
Chr5 mRNA 26364341 26365603 26364341 ID=MsG0580025899.01.T01;Parent=MsG0580025899.01;Name=MsG0580025899.01.T01;_AED=0.48;_eAED=0.52;_QI=0|1|0.33|1|0|0.66|3|0|100
Chr5 exon 26365502 26365603 26365502 ID=MsG0580025899.01.T01:exon:4340;Parent=MsG0580025899.01.T01
Chr5 exon 26365336 26365407 26365336 ID=MsG0580025899.01.T01:exon:4339;Parent=MsG0580025899.01.T01
Chr5 exon 26364341 26364469 26364341 ID=MsG0580025899.01.T01:exon:4338;Parent=MsG0580025899.01.T01
Chr5 CDS 26365502 26365603 26365502 ID=MsG0580025899.01.T01:cds;Parent=MsG0580025899.01.T01
Chr5 CDS 26365336 26365407 26365336 ID=MsG0580025899.01.T01:cds;Parent=MsG0580025899.01.T01
Chr5 CDS 26364341 26364469 26364341 ID=MsG0580025899.01.T01:cds;Parent=MsG0580025899.01.T01
Gene Sequence

>MsG0580025899.01.T01

ATGAATAGGCCTGCTACCGTTGAGAGGATGGAGAAAAAAAGTGGAAGTGTTGTACGAACTTTGGCCCCGAAAAGTGACATTAAATCAGAGAAAGGGAAAGCGTCCTCAAGAAAAATAGAGGAAAAACCTAATACAAAAGTGGTAGAAAGAACGCGTCTCCAAACAAAATTGAAGGAGGAAAAAGAGGCAGAGATGAAAAAGCTAAAGCATGATTTCAAGGCCACACCCCTAACAACTTCTTATCGTGGTCAAAAAGGATCAAAAAGTCGCGCAGAAAAGGTACGTGTTATACATCTGAACTGA

Protein sequence

>MsG0580025899.01.T01

MNRPATVERMEKKSGSVVRTLAPKSDIKSEKGKASSRKIEEKPNTKVVERTRLQTKLKEEKEAEMKKLKHDFKATPLTTSYRGQKGSKSRAEKVRVIHLN*