AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280010813.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010813.01.T01 MTR_2g091110 87.097 62 8 0 1 62 349 410 2.37e-26 100
MsG0280010813.01.T01 MTR_5g078500 63.934 61 22 0 6 66 242 302 1.29e-15 68.9
MsG0280010813.01.T01 MTR_2g091250 68.966 58 18 0 6 63 255 312 1.98e-15 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 9 sgRNAs with CRISPR-Local

Find 10 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGAAGCACTTTGAAGAAT+GGG 0.450476 2:+75368912 MsG0280010813.01.T01:CDS
GCAGAGTGAAGGACGAGCGC+AGG 0.460622 2:+75368876 MsG0280010813.01.T01:CDS
TGAAGAATGGGCAAAGAAGC+TGG 0.468676 2:+75368924 MsG0280010813.01.T01:CDS
AGGAGAAGCACTTTGAAGAA+TGG 0.469828 2:+75368911 MsG0280010813.01.T01:CDS
TTAATTCAAATTCAATCAAA+CGG 0.471300 2:-75368972 None:intergenic
AGCGCAGGAGCTTCAGTCGA+AGG 0.511888 2:+75368891 MsG0280010813.01.T01:CDS
TGAATTTGAATTAAACGAGA+AGG 0.532623 2:+75368981 MsG0280010813.01.T01:CDS
TTAAACGAGAAGGAACTTGA+AGG 0.586614 2:+75368991 MsG0280010813.01.T01:CDS
TTAAAAGAGAAGCAGAGTGA+AGG 0.624724 2:+75368865 MsG0280010813.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATTCAAATTCAATCAAA+CGG - Chr2:75368975-75368994 None:intergenic 15.0%
! TGAATTTGAATTAAACGAGA+AGG + Chr2:75368981-75369000 MsG0280010813.01.T01:CDS 25.0%
! TAAAAAGAGAAGCAAGTTGA+AGG + Chr2:75369032-75369051 MsG0280010813.01.T01:CDS 30.0%
TTAAAAGAGAAGCAGAGTGA+AGG + Chr2:75368865-75368884 MsG0280010813.01.T01:CDS 35.0%
TTAAACGAGAAGGAACTTGA+AGG + Chr2:75368991-75369010 MsG0280010813.01.T01:CDS 35.0%
!! AGGAGAAGCACTTTGAAGAA+TGG + Chr2:75368911-75368930 MsG0280010813.01.T01:CDS 40.0%
!! GGAGAAGCACTTTGAAGAAT+GGG + Chr2:75368912-75368931 MsG0280010813.01.T01:CDS 40.0%
TGAAGAATGGGCAAAGAAGC+TGG + Chr2:75368924-75368943 MsG0280010813.01.T01:CDS 45.0%
AGCGCAGGAGCTTCAGTCGA+AGG + Chr2:75368891-75368910 MsG0280010813.01.T01:CDS 60.0%
GCAGAGTGAAGGACGAGCGC+AGG + Chr2:75368876-75368895 MsG0280010813.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 75368853 75369065 75368853 ID=MsG0280010813.01;Name=MsG0280010813.01
Chr2 mRNA 75368853 75369065 75368853 ID=MsG0280010813.01.T01;Parent=MsG0280010813.01;Name=MsG0280010813.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|70
Chr2 exon 75368853 75369065 75368853 ID=MsG0280010813.01.T01:exon:11629;Parent=MsG0280010813.01.T01
Chr2 CDS 75368853 75369065 75368853 ID=MsG0280010813.01.T01:cds;Parent=MsG0280010813.01.T01
Gene Sequence

>MsG0280010813.01.T01

ATGAAGCTAGTGTTAAAAGAGAAGCAGAGTGAAGGACGAGCGCAGGAGCTTCAGTCGAAGGAGAAGCACTTTGAAGAATGGGCAAAGAAGCTGGAGTCAAGAGAGAAGCAGCTTGATGTCCGTTTGATTGAATTTGAATTAAACGAGAAGGAACTTGAAGGTCGTATGAATGAGCGTGATAAAAAGAGAAGCAAGTTGAAGGACGAGCAATAG

Protein sequence

>MsG0280010813.01.T01

MKLVLKEKQSEGRAQELQSKEKHFEEWAKKLESREKQLDVRLIEFELNEKELEGRMNERDKKRSKLKDEQ*