AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1003750


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 28 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTATCTTGGGATTTGTCTT+TGG 0.274219 7_1:-809280 Msa1003750:CDS
TCAGTAAAGTTACATTATCT+TGG 0.328279 7_1:-809293 Msa1003750:CDS
CTGATAATGAAGTTAGGGTT+TGG 0.352667 7_1:+809729 None:intergenic
GATGGCATGTTGTTGCTTCA+TGG 0.372099 7_1:-809410 Msa1003750:exon
ATGTTGTTGCTTCATGGACT+TGG 0.375530 7_1:-809404 Msa1003750:CDS
TGTCCTGATTGTAAACTTCC+TGG 0.394301 7_1:-809330 Msa1003750:CDS
ATACATACGTTTGAATTTGC+GGG 0.399310 7_1:-809831 Msa1003750:five_prime_UTR
AAAGAAGATTTGAATTGAAA+AGG 0.400354 7_1:+809755 None:intergenic
CTATTGGATTGGATTTCTGC+TGG 0.407759 7_1:-809608 Msa1003750:five_prime_UTR
TATACATACGTTTGAATTTG+CGG 0.415740 7_1:-809832 Msa1003750:five_prime_UTR
ATTTGTAGGATGGCCTTTGA+TGG 0.417636 7_1:-809357 Msa1003750:CDS
CAGTAAAGTTACATTATCTT+GGG 0.422158 7_1:-809292 Msa1003750:CDS
GTCCTGATTGTAAACTTCCT+GGG 0.462403 7_1:-809329 Msa1003750:CDS
ACTAGACAAGACAAAGGAAA+AGG 0.484810 7_1:-809808 Msa1003750:five_prime_UTR
ATGAAACATGTGGAATTTGT+AGG 0.488299 7_1:-809371 Msa1003750:CDS
TTTGAAGCAAAAGCACAAAC+AGG 0.490100 7_1:+809659 None:intergenic
TGTTGTTGCTTCATGGACTT+GGG 0.505473 7_1:-809403 Msa1003750:CDS
TCCCCAGGAAGTTTACAATC+AGG 0.518100 7_1:+809327 None:intergenic
TTCATGGACTTGGGATGCAC+AGG 0.541212 7_1:-809394 Msa1003750:CDS
GATTTGAATTGAAAAGGAGA+AGG 0.548938 7_1:+809761 None:intergenic
TCCTGATTGTAAACTTCCTG+GGG 0.574605 7_1:-809328 Msa1003750:CDS
CTGAGTGGACAGTCATCCCC+AGG 0.575217 7_1:+809312 None:intergenic
ATCAGGACAGCATCCATCAA+AGG 0.590910 7_1:+809344 None:intergenic
CTAGACAAGACAAAGGAAAA+GGG 0.599308 7_1:-809807 Msa1003750:five_prime_UTR
GATAATGTAACTTTACTGAG+TGG 0.670206 7_1:+809297 None:intergenic
TGCGGGACTAGACAAGACAA+AGG 0.697609 7_1:-809814 Msa1003750:five_prime_UTR
AACATGTGGAATTTGTAGGA+TGG 0.713439 7_1:-809367 Msa1003750:CDS
GATGCACAGGATGAAACATG+TGG 0.727134 7_1:-809381 Msa1003750:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAACTAATGAATAAATATT+AGG + chr7_1:809635-809654 None:intergenic 15.0%
!! AAAGAAGATTTGAATTGAAA+AGG + chr7_1:809354-809373 None:intergenic 20.0%
!!! ATTTTCTGATAATGAAGTTA+GGG + chr7_1:809385-809404 None:intergenic 20.0%
! TATACATACGTTTGAATTTG+CGG - chr7_1:809274-809293 Msa1003750:CDS 25.0%
!!! CATTTTCTGATAATGAAGTT+AGG + chr7_1:809386-809405 None:intergenic 25.0%
! ATCTCTAACTAACTTTATAC+AGG - chr7_1:809537-809556 Msa1003750:intron 25.0%
!!! AATATTAGGTTGATTTCACT+AGG + chr7_1:809621-809640 None:intergenic 25.0%
! TCAGTAAAGTTACATTATCT+TGG - chr7_1:809813-809832 Msa1003750:five_prime_UTR 25.0%
! CAGTAAAGTTACATTATCTT+GGG - chr7_1:809814-809833 Msa1003750:five_prime_UTR 25.0%
ATACATACGTTTGAATTTGC+GGG - chr7_1:809275-809294 Msa1003750:CDS 30.0%
GATTTGAATTGAAAAGGAGA+AGG + chr7_1:809348-809367 None:intergenic 30.0%
GGTTGAATGCAAAAATGAAA+AGG + chr7_1:809429-809448 None:intergenic 30.0%
!! TGACTTAGTTTTCTCTCTAT+TGG - chr7_1:809482-809501 Msa1003750:intron 30.0%
! TAGTTTTCTCTCTATTGGAT+TGG - chr7_1:809487-809506 Msa1003750:intron 30.0%
CTAACTTTATACAGGTTGTT+TGG - chr7_1:809545-809564 Msa1003750:intron 30.0%
TCTCTTATCTCAAATGTAGA+TGG - chr7_1:809678-809697 Msa1003750:five_prime_UTR 30.0%
ATGAAACATGTGGAATTTGT+AGG - chr7_1:809735-809754 Msa1003750:five_prime_UTR 30.0%
GATAATGTAACTTTACTGAG+TGG + chr7_1:809812-809831 None:intergenic 30.0%
ATTATCTTGGGATTTGTCTT+TGG - chr7_1:809826-809845 Msa1003750:five_prime_UTR 30.0%
ACTAGACAAGACAAAGGAAA+AGG - chr7_1:809298-809317 Msa1003750:CDS 35.0%
CTAGACAAGACAAAGGAAAA+GGG - chr7_1:809299-809318 Msa1003750:CDS 35.0%
! CTGATAATGAAGTTAGGGTT+TGG + chr7_1:809380-809399 None:intergenic 35.0%
! TTTGAAGCAAAAGCACAAAC+AGG + chr7_1:809450-809469 None:intergenic 35.0%
AACATGTGGAATTTGTAGGA+TGG - chr7_1:809739-809758 Msa1003750:five_prime_UTR 35.0%
! CTATTGGATTGGATTTCTGC+TGG - chr7_1:809498-809517 Msa1003750:intron 40.0%
CTTTATACAGGTTGTTTGGC+TGG - chr7_1:809549-809568 Msa1003750:intron 40.0%
! ATGTTGTTGCTTCATGGACT+TGG - chr7_1:809702-809721 Msa1003750:five_prime_UTR 40.0%
! TGTTGTTGCTTCATGGACTT+GGG - chr7_1:809703-809722 Msa1003750:five_prime_UTR 40.0%
ATTTGTAGGATGGCCTTTGA+TGG - chr7_1:809749-809768 Msa1003750:five_prime_UTR 40.0%
TGTCCTGATTGTAAACTTCC+TGG - chr7_1:809776-809795 Msa1003750:five_prime_UTR 40.0%
GTCCTGATTGTAAACTTCCT+GGG - chr7_1:809777-809796 Msa1003750:five_prime_UTR 40.0%
TCCTGATTGTAAACTTCCTG+GGG - chr7_1:809778-809797 Msa1003750:five_prime_UTR 40.0%
! GATGGCATGTTGTTGCTTCA+TGG - chr7_1:809696-809715 Msa1003750:five_prime_UTR 45.0%
GATGCACAGGATGAAACATG+TGG - chr7_1:809725-809744 Msa1003750:five_prime_UTR 45.0%
ATCAGGACAGCATCCATCAA+AGG + chr7_1:809765-809784 None:intergenic 45.0%
TCCCCAGGAAGTTTACAATC+AGG + chr7_1:809782-809801 None:intergenic 45.0%
TGCGGGACTAGACAAGACAA+AGG - chr7_1:809292-809311 Msa1003750:CDS 50.0%
TACAGGTTGTTTGGCTGGTG+TGG - chr7_1:809554-809573 Msa1003750:intron 50.0%
TTGTTTGGCTGGTGTGGTTC+TGG - chr7_1:809560-809579 Msa1003750:intron 50.0%
TTCATGGACTTGGGATGCAC+AGG - chr7_1:809712-809731 Msa1003750:five_prime_UTR 50.0%
CTGAGTGGACAGTCATCCCC+AGG + chr7_1:809797-809816 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 809274 809854 809274 ID=Msa1003750;Name=Msa1003750
chr7_1 mRNA 809274 809854 809274 ID=Msa1003750-mRNA-1;Parent=Msa1003750;Name=Msa1003750-mRNA-1;_AED=0.00;_eAED=0.00;_QI=261|1|0.5|1|1|0.5|2|0|51
chr7_1 exon 809274 809431 809274 ID=Msa1003750-mRNA-1:exon:282;Parent=Msa1003750-mRNA-1
chr7_1 exon 809599 809854 809599 ID=Msa1003750-mRNA-1:exon:281;Parent=Msa1003750-mRNA-1
chr7_1 five_prime_UTR 809599 809854 809599 ID=Msa1003750-mRNA-1:five_prime_utr;Parent=Msa1003750-mRNA-1
chr7_1 five_prime_UTR 809427 809431 809427 ID=Msa1003750-mRNA-1:five_prime_utr;Parent=Msa1003750-mRNA-1
chr7_1 CDS 809274 809426 809274 ID=Msa1003750-mRNA-1:cds;Parent=Msa1003750-mRNA-1
Gene Sequence

>Msa1003750

ATGTTGTTGCTTCATGGACTTGGGATGCACAGGATGAAACATGTGGAATTTGTAGGATGGCCTTTGATGGATGCTGTCCTGATTGTAAACTTCCTGGGGATGACTGTCCACTCAGTAAAGTTACATTATCTTGGGATTTGTCTTTGGTTAATC

Protein sequence

>Msa1003750

MLLLHGLGMHRMKHVEFVGWPLMDAVLIVNFLGMTVHSVKLHYLGICLWLI