AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0006380


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0006380 MtrunA17_Chr3g0137181 100.000 51 0 0 36 86 1 51 9.40e-33 107
Msa0006380 MtrunA17_Chr1g0149761 100.000 51 0 0 36 86 1 51 9.40e-33 107
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0006380 AT4G31985.1 90.196 51 5 0 36 86 1 51 1.39e-29 100
Msa0006380 AT3G02190.1 88.235 51 6 0 36 86 1 51 3.59e-28 96.7
Msa0006380 AT2G25210.1 90.909 44 4 0 43 86 1 44 4.89e-24 85.9

Find 33 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCCACATTTGAGCCGATTT+TGG 0.217972 1_1:-9244685 Msa0006380:three_prime_UTR
CCTTCTTCTTCGGCTGCGTC+TGG 0.331724 1_1:+9245358 None:intergenic
ACGGCGATACCAAACCCTAA+TGG 0.370707 1_1:+9245399 None:intergenic
AGAGGTCGAGTAACCCATTA+GGG 0.375345 1_1:-9245413 Msa0006380:CDS
TCTTCTTCTTCCTTCTTCTT+CGG 0.378925 1_1:+9245348 None:intergenic
CGGCGATACCAAACCCTAAT+GGG 0.392508 1_1:+9245400 None:intergenic
CAAACAGCTTTCACCAAAAT+CGG 0.399033 1_1:+9244672 None:intergenic
GTTCTCATACGGATCCAATA+AGG 0.402869 1_1:+9245143 None:intergenic
AAGAGGTCGAGTAACCCATT+AGG 0.404554 1_1:-9245414 Msa0006380:CDS
AGAACAGACCCATCCCTTAT+TGG 0.418161 1_1:-9245157 Msa0006380:CDS
CACTGGCGTCGTACCAAGCT+TGG 0.445699 1_1:-9244990 Msa0006380:CDS
TCGAGTAACCCATTAGGGTT+TGG 0.465477 1_1:-9245408 Msa0006380:CDS
TTCTCATACGGATCCAATAA+GGG 0.468482 1_1:+9245144 None:intergenic
GAGATCAATGTTGTTATGTA+AGG 0.485401 1_1:-9244863 Msa0006380:three_prime_UTR
AATTAACCTGATGGTGTTGT+CGG 0.494295 1_1:+9245121 None:intergenic
TTAGGGTTTGGTATCGCCGT+TGG 0.497744 1_1:-9245396 Msa0006380:CDS
CACCAAAATCGGCTCAAATG+TGG 0.498408 1_1:+9244683 None:intergenic
TTGTTATGTAAGGAAGTTTG+CGG 0.501796 1_1:-9244853 Msa0006380:three_prime_UTR
TGAGAACCGACAACACCATC+AGG 0.505441 1_1:-9245127 Msa0006380:intron
ACCAAAATCGGCTCAAATGT+GGG 0.509392 1_1:+9244684 None:intergenic
TTTGTTATAATAGTCTGTCT+AGG 0.516766 1_1:-9244653 Msa0006380:three_prime_UTR
CGTACCAAGCTTGGATTCTG+AGG 0.522895 1_1:-9244981 Msa0006380:exon
CGCTTAATAAAATAAAATGC+GGG 0.529617 1_1:+9244822 None:intergenic
TGGTGTTGTCGGTTCTCATA+CGG 0.540409 1_1:+9245132 None:intergenic
ACAATGCTAAGCGTCGTCAC+TGG 0.548403 1_1:-9245007 Msa0006380:CDS
AAAACCTCAGAATCCAAGCT+TGG 0.552557 1_1:+9244977 None:intergenic
CCAGACGCAGCCGAAGAAGA+AGG 0.553197 1_1:-9245358 Msa0006380:CDS
AGAAGCTCGCGAAGAAGATG+AGG 0.578712 1_1:-9245181 Msa0006380:CDS
CATACGGATCCAATAAGGGA+TGG 0.584103 1_1:+9245148 None:intergenic
ATGACGAATGTCTTGTGTGA+AGG 0.588940 1_1:+9245209 None:intergenic
ATACGGATCCAATAAGGGAT+GGG 0.595390 1_1:+9245149 None:intergenic
TCGCTTAATAAAATAAAATG+CGG 0.603548 1_1:+9244821 None:intergenic
GAGATGAAATGCTGAACCAA+CGG 0.639403 1_1:+9245380 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATTTTATTTTATTTTTT+AGG + chr1_1:9244633-9244652 None:intergenic 0.0%
!!! TTTATTTTATTTTATTTTTT+AGG + chr1_1:9244633-9244652 None:intergenic 0.0%
!!! ATGTTATTTCATTTGATTTT+AGG - chr1_1:9245068-9245087 Msa0006380:intron 15.0%
!!! ATGTTATTTCATTTGATTTT+AGG - chr1_1:9245068-9245087 Msa0006380:intron 15.0%
!! TCGCTTAATAAAATAAAATG+CGG + chr1_1:9245281-9245300 None:intergenic 20.0%
!! TCGCTTAATAAAATAAAATG+CGG + chr1_1:9245281-9245300 None:intergenic 20.0%
! ATAGATAGAAATTAACCTGA+TGG + chr1_1:9244990-9245009 None:intergenic 25.0%
!!! TTCTGAGGTTTTTTAGTTTA+TGG - chr1_1:9245133-9245152 Msa0006380:CDS 25.0%
!!! TCTGAGGTTTTTTAGTTTAT+GGG - chr1_1:9245134-9245153 Msa0006380:CDS 25.0%
! CGCTTAATAAAATAAAATGC+GGG + chr1_1:9245280-9245299 None:intergenic 25.0%
!!! TTATTTTATTAAGCGACTTG+TGG - chr1_1:9245285-9245304 Msa0006380:intron 25.0%
!!! CTTGTGATTTTGATTTTGTT+AGG - chr1_1:9245318-9245337 Msa0006380:intron 25.0%
!!! TTTGATTTTGTTAGGTAGAA+TGG - chr1_1:9245326-9245345 Msa0006380:intron 25.0%
!! TTTGTTATAATAGTCTGTCT+AGG - chr1_1:9245446-9245465 Msa0006380:intron 25.0%
! ATAGATAGAAATTAACCTGA+TGG + chr1_1:9244990-9245009 None:intergenic 25.0%
!!! TTCTGAGGTTTTTTAGTTTA+TGG - chr1_1:9245133-9245152 Msa0006380:CDS 25.0%
!!! TCTGAGGTTTTTTAGTTTAT+GGG - chr1_1:9245134-9245153 Msa0006380:CDS 25.0%
! CGCTTAATAAAATAAAATGC+GGG + chr1_1:9245280-9245299 None:intergenic 25.0%
!!! TTATTTTATTAAGCGACTTG+TGG - chr1_1:9245285-9245304 Msa0006380:intron 25.0%
!!! CTTGTGATTTTGATTTTGTT+AGG - chr1_1:9245318-9245337 Msa0006380:intron 25.0%
!!! TTTGATTTTGTTAGGTAGAA+TGG - chr1_1:9245326-9245345 Msa0006380:intron 25.0%
!! TTTGTTATAATAGTCTGTCT+AGG - chr1_1:9245446-9245465 Msa0006380:intron 25.0%
ATAACGAGAGCTATAAAAAG+AGG - chr1_1:9244668-9244687 Msa0006380:three_prime_UTR 30.0%
AGAATAGTGAAATTAGGAGT+GGG + chr1_1:9244826-9244845 None:intergenic 30.0%
GAATCGAGAATAGTGAAATT+AGG + chr1_1:9244832-9244851 None:intergenic 30.0%
!!! GACAGTGAATTTTGTTGTTT+TGG - chr1_1:9245178-9245197 Msa0006380:CDS 30.0%
GAGATCAATGTTGTTATGTA+AGG - chr1_1:9245236-9245255 Msa0006380:intron 30.0%
TTGTTATGTAAGGAAGTTTG+CGG - chr1_1:9245246-9245265 Msa0006380:intron 30.0%
! ATTTTGTTAGGTAGAATGGA+TGG - chr1_1:9245330-9245349 Msa0006380:intron 30.0%
ATAACGAGAGCTATAAAAAG+AGG - chr1_1:9244668-9244687 Msa0006380:three_prime_UTR 30.0%
AGAATAGTGAAATTAGGAGT+GGG + chr1_1:9244826-9244845 None:intergenic 30.0%
GAATCGAGAATAGTGAAATT+AGG + chr1_1:9244832-9244851 None:intergenic 30.0%
!!! GACAGTGAATTTTGTTGTTT+TGG - chr1_1:9245178-9245197 Msa0006380:CDS 30.0%
GAGATCAATGTTGTTATGTA+AGG - chr1_1:9245236-9245255 Msa0006380:intron 30.0%
TTGTTATGTAAGGAAGTTTG+CGG - chr1_1:9245246-9245265 Msa0006380:intron 30.0%
! ATTTTGTTAGGTAGAATGGA+TGG - chr1_1:9245330-9245349 Msa0006380:intron 30.0%
TCTTCTTCTTCCTTCTTCTT+CGG + chr1_1:9244754-9244773 None:intergenic 35.0%
AGAAGAAGAAGCTGCAAAAA+TGG - chr1_1:9244768-9244787 Msa0006380:three_prime_UTR 35.0%
TGAAATTAGGAGTGGGAATT+TGG + chr1_1:9244819-9244838 None:intergenic 35.0%
GAGAATAGTGAAATTAGGAG+TGG + chr1_1:9244827-9244846 None:intergenic 35.0%
TTCTCATACGGATCCAATAA+GGG + chr1_1:9244958-9244977 None:intergenic 35.0%
!! AATTAACCTGATGGTGTTGT+CGG + chr1_1:9244981-9245000 None:intergenic 35.0%
TTAACAGTACAACGTTGTTG+TGG + chr1_1:9245018-9245037 None:intergenic 35.0%
CAAACAGCTTTCACCAAAAT+CGG + chr1_1:9245430-9245449 None:intergenic 35.0%
TCTTCTTCTTCCTTCTTCTT+CGG + chr1_1:9244754-9244773 None:intergenic 35.0%
AGAAGAAGAAGCTGCAAAAA+TGG - chr1_1:9244768-9244787 Msa0006380:three_prime_UTR 35.0%
TGAAATTAGGAGTGGGAATT+TGG + chr1_1:9244819-9244838 None:intergenic 35.0%
GAGAATAGTGAAATTAGGAG+TGG + chr1_1:9244827-9244846 None:intergenic 35.0%
TTCTCATACGGATCCAATAA+GGG + chr1_1:9244958-9244977 None:intergenic 35.0%
!! AATTAACCTGATGGTGTTGT+CGG + chr1_1:9244981-9245000 None:intergenic 35.0%
TTAACAGTACAACGTTGTTG+TGG + chr1_1:9245018-9245037 None:intergenic 35.0%
CAAACAGCTTTCACCAAAAT+CGG + chr1_1:9245430-9245449 None:intergenic 35.0%
GAGATGAAATGCTGAACCAA+CGG + chr1_1:9244722-9244741 None:intergenic 40.0%
ATGACGAATGTCTTGTGTGA+AGG + chr1_1:9244893-9244912 None:intergenic 40.0%
ATACGGATCCAATAAGGGAT+GGG + chr1_1:9244953-9244972 None:intergenic 40.0%
GTTCTCATACGGATCCAATA+AGG + chr1_1:9244959-9244978 None:intergenic 40.0%
AAAACCTCAGAATCCAAGCT+TGG + chr1_1:9245125-9245144 None:intergenic 40.0%
! ACCAAAATCGGCTCAAATGT+GGG + chr1_1:9245418-9245437 None:intergenic 40.0%
GAGATGAAATGCTGAACCAA+CGG + chr1_1:9244722-9244741 None:intergenic 40.0%
ATGACGAATGTCTTGTGTGA+AGG + chr1_1:9244893-9244912 None:intergenic 40.0%
ATACGGATCCAATAAGGGAT+GGG + chr1_1:9244953-9244972 None:intergenic 40.0%
GTTCTCATACGGATCCAATA+AGG + chr1_1:9244959-9244978 None:intergenic 40.0%
AAAACCTCAGAATCCAAGCT+TGG + chr1_1:9245125-9245144 None:intergenic 40.0%
! ACCAAAATCGGCTCAAATGT+GGG + chr1_1:9245418-9245437 None:intergenic 40.0%
AAGAGGTCGAGTAACCCATT+AGG - chr1_1:9244685-9244704 Msa0006380:three_prime_UTR 45.0%
AGAGGTCGAGTAACCCATTA+GGG - chr1_1:9244686-9244705 Msa0006380:three_prime_UTR 45.0%
TCGAGTAACCCATTAGGGTT+TGG - chr1_1:9244691-9244710 Msa0006380:three_prime_UTR 45.0%
AGAACAGACCCATCCCTTAT+TGG - chr1_1:9244942-9244961 Msa0006380:three_prime_UTR 45.0%
CATACGGATCCAATAAGGGA+TGG + chr1_1:9244954-9244973 None:intergenic 45.0%
!! TGGTGTTGTCGGTTCTCATA+CGG + chr1_1:9244970-9244989 None:intergenic 45.0%
!! TCCCACATTTGAGCCGATTT+TGG - chr1_1:9245414-9245433 Msa0006380:CDS 45.0%
! CACCAAAATCGGCTCAAATG+TGG + chr1_1:9245419-9245438 None:intergenic 45.0%
AAGAGGTCGAGTAACCCATT+AGG - chr1_1:9244685-9244704 Msa0006380:three_prime_UTR 45.0%
AGAGGTCGAGTAACCCATTA+GGG - chr1_1:9244686-9244705 Msa0006380:three_prime_UTR 45.0%
TCGAGTAACCCATTAGGGTT+TGG - chr1_1:9244691-9244710 Msa0006380:three_prime_UTR 45.0%
AGAACAGACCCATCCCTTAT+TGG - chr1_1:9244942-9244961 Msa0006380:three_prime_UTR 45.0%
CATACGGATCCAATAAGGGA+TGG + chr1_1:9244954-9244973 None:intergenic 45.0%
!! TGGTGTTGTCGGTTCTCATA+CGG + chr1_1:9244970-9244989 None:intergenic 45.0%
!! TCCCACATTTGAGCCGATTT+TGG - chr1_1:9245414-9245433 Msa0006380:CDS 45.0%
! CACCAAAATCGGCTCAAATG+TGG + chr1_1:9245419-9245438 None:intergenic 45.0%
CGGCGATACCAAACCCTAAT+GGG + chr1_1:9244702-9244721 None:intergenic 50.0%
ACGGCGATACCAAACCCTAA+TGG + chr1_1:9244703-9244722 None:intergenic 50.0%
! TTAGGGTTTGGTATCGCCGT+TGG - chr1_1:9244703-9244722 Msa0006380:three_prime_UTR 50.0%
AGAAGCTCGCGAAGAAGATG+AGG - chr1_1:9244918-9244937 Msa0006380:three_prime_UTR 50.0%
TGAGAACCGACAACACCATC+AGG - chr1_1:9244972-9244991 Msa0006380:exon 50.0%
! ACAATGCTAAGCGTCGTCAC+TGG - chr1_1:9245092-9245111 Msa0006380:intron 50.0%
CGTACCAAGCTTGGATTCTG+AGG - chr1_1:9245118-9245137 Msa0006380:intron 50.0%
CGGCGATACCAAACCCTAAT+GGG + chr1_1:9244702-9244721 None:intergenic 50.0%
ACGGCGATACCAAACCCTAA+TGG + chr1_1:9244703-9244722 None:intergenic 50.0%
! TTAGGGTTTGGTATCGCCGT+TGG - chr1_1:9244703-9244722 Msa0006380:three_prime_UTR 50.0%
AGAAGCTCGCGAAGAAGATG+AGG - chr1_1:9244918-9244937 Msa0006380:three_prime_UTR 50.0%
TGAGAACCGACAACACCATC+AGG - chr1_1:9244972-9244991 Msa0006380:exon 50.0%
! ACAATGCTAAGCGTCGTCAC+TGG - chr1_1:9245092-9245111 Msa0006380:intron 50.0%
CGTACCAAGCTTGGATTCTG+AGG - chr1_1:9245118-9245137 Msa0006380:intron 50.0%
CCTTCTTCTTCGGCTGCGTC+TGG + chr1_1:9244744-9244763 None:intergenic 60.0%
CCAGACGCAGCCGAAGAAGA+AGG - chr1_1:9244741-9244760 Msa0006380:three_prime_UTR 60.0%
CACTGGCGTCGTACCAAGCT+TGG - chr1_1:9245109-9245128 Msa0006380:intron 60.0%
CCTTCTTCTTCGGCTGCGTC+TGG + chr1_1:9244744-9244763 None:intergenic 60.0%
CCAGACGCAGCCGAAGAAGA+AGG - chr1_1:9244741-9244760 Msa0006380:three_prime_UTR 60.0%
CACTGGCGTCGTACCAAGCT+TGG - chr1_1:9245109-9245128 Msa0006380:intron 60.0%
Chromosome Type Strat End Strand Name
chr1_1 gene 9244618 9245503 9244618 ID=Msa0006380;Name=Msa0006380
chr1_1 mRNA 9244618 9245503 9244618 ID=Msa0006380-mRNA-1;Parent=Msa0006380;Name=Msa0006380-mRNA-1;_AED=0.08;_eAED=0.08;_QI=0|0.66|0.5|0.75|1|1|4|365|86
chr1_1 exon 9244618 9245031 9244618 ID=Msa0006380-mRNA-1:exon:3280;Parent=Msa0006380-mRNA-1
chr1_1 exon 9245128 9245231 9245128 ID=Msa0006380-mRNA-1:exon:3279;Parent=Msa0006380-mRNA-1
chr1_1 exon 9245332 9245433 9245332 ID=Msa0006380-mRNA-1:exon:3278;Parent=Msa0006380-mRNA-1
chr1_1 exon 9245498 9245503 9245498 ID=Msa0006380-mRNA-1:exon:3277;Parent=Msa0006380-mRNA-1
chr1_1 CDS 9245498 9245503 9245498 ID=Msa0006380-mRNA-1:cds;Parent=Msa0006380-mRNA-1
chr1_1 CDS 9245332 9245433 9245332 ID=Msa0006380-mRNA-1:cds;Parent=Msa0006380-mRNA-1
chr1_1 CDS 9245128 9245231 9245128 ID=Msa0006380-mRNA-1:cds;Parent=Msa0006380-mRNA-1
chr1_1 CDS 9244983 9245031 9244983 ID=Msa0006380-mRNA-1:cds;Parent=Msa0006380-mRNA-1
chr1_1 three_prime_UTR 9244618 9244982 9244618 ID=Msa0006380-mRNA-1:three_prime_utr;Parent=Msa0006380-mRNA-1
Gene Sequence

>Msa0006380

ATGAGAAGGTCGAGTAACCCATTAGGGTTTGGTATCGCCGTTGGTTCAGCATTTCATCTCCAGACGCAGCCGAAGAAGAAGGAAGAAGAAGAAGAAGCTGCAAAAATGCCTTCACACAAGACATTCGTCATCAAGAAGAAGCTCGCGAAGAAGATGAGGCAGAACAGACCCATCCCTTATTGGATCCGTATGAGAACCGACAACACCATCAGGTACAATGCTAAGCGTCGTCACTGGCGTCGTACCAAGCTTGGATTCTGA

Protein sequence

>Msa0006380

MRRSSNPLGFGIAVGSAFHLQTQPKKKEEEEEAAKMPSHKTFVIKKKLAKKMRQNRPIPYWIRMRTDNTIRYNAKRRHWRRTKLGF*