AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0372440


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0372440 MtrunA17_Chr3g0120091 97.674 43 1 0 32 74 1 43 6.90e-14 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 32 sgRNAs with CRISPR-Local

Find 138 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTTAGAGTCCTCAAAATT+TGG 0.135898 3_1:+70896943 None:intergenic
TCCCACTTCCATTCCTTCTT+AGG 0.263163 3_1:+70897570 None:intergenic
AGTTCAAACAGTTTGGCTTC+TGG 0.307622 3_1:+70897927 None:intergenic
TGGGAGTTCCAGCTCAAGTT+TGG 0.326309 3_1:-70897552 Msa0372440:CDS
CTTTAGAGTCCTCAAAATTT+GGG 0.336690 3_1:+70896944 None:intergenic
GCCATGTTGTGGTTGGGATC+AGG 0.350494 3_1:-70897034 Msa0372440:CDS
ATGTGCAACATGGGCTATTA+TGG 0.360481 3_1:-70896998 Msa0372440:CDS
TACATAGAAGCAGCCGTGTT+TGG 0.417611 3_1:-70897963 Msa0372440:CDS
GGGAGTTCCAGCTCAAGTTT+GGG 0.421121 3_1:-70897551 Msa0372440:CDS
CCATGTTGTGGTTGGGATCA+GGG 0.449639 3_1:-70897033 Msa0372440:CDS
GGCCATCTGCATGTGCAACA+TGG 0.482270 3_1:-70897008 Msa0372440:CDS
ATGTTGCACATGCAGATGGC+CGG 0.495389 3_1:+70897010 None:intergenic
GCTGCTGCCCAAACTTGAGC+TGG 0.496231 3_1:+70897544 None:intergenic
TCATCAGCCATGTTGTGGTT+GGG 0.501070 3_1:-70897040 Msa0372440:CDS
GTCATCAGCCATGTTGTGGT+TGG 0.508937 3_1:-70897041 Msa0372440:CDS
ACAGAGACTCAAAATGTTGA+GGG 0.528820 3_1:-70896974 Msa0372440:CDS
GTTGTGGTTGGGATCAGGGC+CGG 0.544933 3_1:-70897029 Msa0372440:CDS
AACCTAAGAAGGAATGGAAG+TGG 0.544966 3_1:-70897572 Msa0372440:CDS
GCCATCTGCATGTGCAACAT+GGG 0.545810 3_1:-70897007 Msa0372440:CDS
GGTGAGAACCTAAGAAGGAA+TGG 0.550083 3_1:-70897578 Msa0372440:CDS
TTGAGCCAACATGTTAGCAA+TGG 0.557382 3_1:+70897493 None:intergenic
CACAGAGACTCAAAATGTTG+AGG 0.567343 3_1:-70896975 Msa0372440:CDS
GCCCATGTTGCACATGCAGA+TGG 0.577384 3_1:+70897006 None:intergenic
CCCTGATCCCAACCACAACA+TGG 0.583449 3_1:+70897033 None:intergenic
TTTGGGCAGCAGCTTTCATG+AGG 0.600879 3_1:-70897534 Msa0372440:CDS
ACCTAAGAAGGAATGGAAGT+GGG 0.611198 3_1:-70897571 Msa0372440:CDS
TCAAACCATTGCTAACATGT+TGG 0.611337 3_1:-70897498 Msa0372440:CDS
GTATGGGTGAGAACCTAAGA+AGG 0.616521 3_1:-70897583 Msa0372440:CDS
AGAAGTCATCAGCCATGTTG+TGG 0.623851 3_1:-70897045 Msa0372440:CDS
CAGCTTTCATGAGGACACAG+AGG 0.724339 3_1:-70897525 Msa0372440:CDS
ATGAAAATAAGACTCAACAG+TGG 0.736068 3_1:-70898005 Msa0372440:CDS
CAAGACGAAACTCTTCACGC+CGG 0.754527 3_1:-70897473 Msa0372440:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAATTATTCTGTTTATTA+AGG - chr3_1:70897208-70897227 Msa0372440:intron 10.0%
!! TAAATTATTCTGTTTATTAA+GGG - chr3_1:70897209-70897228 Msa0372440:intron 10.0%
!! TTAAATTATTCTGTTTATTA+AGG - chr3_1:70897208-70897227 Msa0372440:intron 10.0%
!! TAAATTATTCTGTTTATTAA+GGG - chr3_1:70897209-70897228 Msa0372440:intron 10.0%
!! ATAAACAGAATAATTTAACT+TGG + chr3_1:70897206-70897225 None:intergenic 15.0%
!!! ATAGTTAATTTTGATATGTT+TGG - chr3_1:70897592-70897611 Msa0372440:intron 15.0%
!! ATAAACAGAATAATTTAACT+TGG + chr3_1:70897206-70897225 None:intergenic 15.0%
!!! ATAGTTAATTTTGATATGTT+TGG - chr3_1:70897592-70897611 Msa0372440:intron 15.0%
!!! ATTTGTTTGTAATTGTTGTT+TGG - chr3_1:70897345-70897364 Msa0372440:intron 20.0%
!! AAATTTCAGTGTAAAAATCA+TGG + chr3_1:70897694-70897713 None:intergenic 20.0%
!!! ATTTGTTTGTAATTGTTGTT+TGG - chr3_1:70897345-70897364 Msa0372440:intron 20.0%
!! AAATTTCAGTGTAAAAATCA+TGG + chr3_1:70897694-70897713 None:intergenic 20.0%
! AAGAAATGAAAAACCAAACA+CGG + chr3_1:70897013-70897032 None:intergenic 25.0%
! AAAGAGAAAGAAAGAAAGAA+GGG + chr3_1:70897095-70897114 None:intergenic 25.0%
! TATTCTGTTTATTAAGGGTA+AGG - chr3_1:70897214-70897233 Msa0372440:intron 25.0%
! ACAACAATTACAAACAAATC+AGG + chr3_1:70897344-70897363 None:intergenic 25.0%
!!! TTGTTTGGTTGATTTTAGTA+TGG - chr3_1:70897360-70897379 Msa0372440:intron 25.0%
!!! TGTTTGGTTGATTTTAGTAT+GGG - chr3_1:70897361-70897380 Msa0372440:intron 25.0%
!!! TTTGTCGCTATAATTGATTT+TGG - chr3_1:70897563-70897582 Msa0372440:CDS 25.0%
! TAAATGTTAAGCTCAACTAA+AGG - chr3_1:70897813-70897832 Msa0372440:intron 25.0%
! AAATGTTAAGCTCAACTAAA+GGG - chr3_1:70897814-70897833 Msa0372440:intron 25.0%
!! TGTAGTTTGTAAAACTGATT+TGG - chr3_1:70897850-70897869 Msa0372440:intron 25.0%
!! GTAGTTTGTAAAACTGATTT+GGG - chr3_1:70897851-70897870 Msa0372440:intron 25.0%
!!! TAGTTTGTAAAACTGATTTG+GGG - chr3_1:70897852-70897871 Msa0372440:intron 25.0%
! AAGAAATGAAAAACCAAACA+CGG + chr3_1:70897013-70897032 None:intergenic 25.0%
! AAAGAGAAAGAAAGAAAGAA+GGG + chr3_1:70897095-70897114 None:intergenic 25.0%
! TATTCTGTTTATTAAGGGTA+AGG - chr3_1:70897214-70897233 Msa0372440:intron 25.0%
! ACAACAATTACAAACAAATC+AGG + chr3_1:70897344-70897363 None:intergenic 25.0%
!!! TTGTTTGGTTGATTTTAGTA+TGG - chr3_1:70897360-70897379 Msa0372440:intron 25.0%
!!! TGTTTGGTTGATTTTAGTAT+GGG - chr3_1:70897361-70897380 Msa0372440:intron 25.0%
!!! TTTGTCGCTATAATTGATTT+TGG - chr3_1:70897563-70897582 Msa0372440:CDS 25.0%
! TAAATGTTAAGCTCAACTAA+AGG - chr3_1:70897813-70897832 Msa0372440:intron 25.0%
! AAATGTTAAGCTCAACTAAA+GGG - chr3_1:70897814-70897833 Msa0372440:intron 25.0%
!! TGTAGTTTGTAAAACTGATT+TGG - chr3_1:70897850-70897869 Msa0372440:intron 25.0%
!! GTAGTTTGTAAAACTGATTT+GGG - chr3_1:70897851-70897870 Msa0372440:intron 25.0%
!!! TAGTTTGTAAAACTGATTTG+GGG - chr3_1:70897852-70897871 Msa0372440:intron 25.0%
ATGAAAATAAGACTCAACAG+TGG - chr3_1:70896955-70896974 Msa0372440:CDS 30.0%
GAAAGAGAAAGAAAGAAAGA+AGG + chr3_1:70897096-70897115 None:intergenic 30.0%
TTATAGCGACAAAAACATAC+TGG + chr3_1:70897556-70897575 None:intergenic 30.0%
!! GTTGACTTTAACTTGAAGTT+AGG - chr3_1:70897648-70897667 Msa0372440:intron 30.0%
TATTCTAACATCTCGTAGTT+TGG + chr3_1:70897793-70897812 None:intergenic 30.0%
TATGATTATGAAACCAGCTT+TGG - chr3_1:70897892-70897911 Msa0372440:intron 30.0%
ATGAAAATAAGACTCAACAG+TGG - chr3_1:70896955-70896974 Msa0372440:CDS 30.0%
GAAAGAGAAAGAAAGAAAGA+AGG + chr3_1:70897096-70897115 None:intergenic 30.0%
TTATAGCGACAAAAACATAC+TGG + chr3_1:70897556-70897575 None:intergenic 30.0%
!! GTTGACTTTAACTTGAAGTT+AGG - chr3_1:70897648-70897667 Msa0372440:intron 30.0%
TATTCTAACATCTCGTAGTT+TGG + chr3_1:70897793-70897812 None:intergenic 30.0%
TATGATTATGAAACCAGCTT+TGG - chr3_1:70897892-70897911 Msa0372440:intron 30.0%
GAGGTGAAGTAAAGAAATGT+AGG + chr3_1:70897073-70897092 None:intergenic 35.0%
TCAAACCATTGCTAACATGT+TGG - chr3_1:70897462-70897481 Msa0372440:intron 35.0%
TACTGGAATGGAATCTAAGT+GGG + chr3_1:70897539-70897558 None:intergenic 35.0%
ATACTGGAATGGAATCTAAG+TGG + chr3_1:70897540-70897559 None:intergenic 35.0%
!! GTCGCTATAATTGATTTTGG+AGG - chr3_1:70897566-70897585 Msa0372440:CDS 35.0%
!! TTTGTAAAACTGATTTGGGG+AGG - chr3_1:70897855-70897874 Msa0372440:intron 35.0%
ACAGAGACTCAAAATGTTGA+GGG - chr3_1:70897986-70898005 Msa0372440:CDS 35.0%
GAGGTGAAGTAAAGAAATGT+AGG + chr3_1:70897073-70897092 None:intergenic 35.0%
TCAAACCATTGCTAACATGT+TGG - chr3_1:70897462-70897481 Msa0372440:intron 35.0%
TACTGGAATGGAATCTAAGT+GGG + chr3_1:70897539-70897558 None:intergenic 35.0%
ATACTGGAATGGAATCTAAG+TGG + chr3_1:70897540-70897559 None:intergenic 35.0%
!! GTCGCTATAATTGATTTTGG+AGG - chr3_1:70897566-70897585 Msa0372440:CDS 35.0%
!! TTTGTAAAACTGATTTGGGG+AGG - chr3_1:70897855-70897874 Msa0372440:intron 35.0%
ACAGAGACTCAAAATGTTGA+GGG - chr3_1:70897986-70898005 Msa0372440:CDS 35.0%
! AGTTCAAACAGTTTGGCTTC+TGG + chr3_1:70897036-70897055 None:intergenic 40.0%
TGGGAAGAGTTCAAACAGTT+TGG + chr3_1:70897043-70897062 None:intergenic 40.0%
AAGAAATGTAGGCGTGTGAT+GGG + chr3_1:70897062-70897081 None:intergenic 40.0%
AAAGAAATGTAGGCGTGTGA+TGG + chr3_1:70897063-70897082 None:intergenic 40.0%
GAGAAAGAAAGAAAGAAGGG+AGG + chr3_1:70897092-70897111 None:intergenic 40.0%
AACCTAAGAAGGAATGGAAG+TGG - chr3_1:70897388-70897407 Msa0372440:intron 40.0%
ACCTAAGAAGGAATGGAAGT+GGG - chr3_1:70897389-70897408 Msa0372440:intron 40.0%
TTGAGCCAACATGTTAGCAA+TGG + chr3_1:70897470-70897489 None:intergenic 40.0%
GAATGGAATCTAAGTGGGAA+AGG + chr3_1:70897534-70897553 None:intergenic 40.0%
GCGACAAAAACATACTGGAA+TGG + chr3_1:70897551-70897570 None:intergenic 40.0%
! ATGTGCAACATGGGCTATTA+TGG - chr3_1:70897962-70897981 Msa0372440:CDS 40.0%
CACAGAGACTCAAAATGTTG+AGG - chr3_1:70897985-70898004 Msa0372440:CDS 40.0%
! AGTTCAAACAGTTTGGCTTC+TGG + chr3_1:70897036-70897055 None:intergenic 40.0%
TGGGAAGAGTTCAAACAGTT+TGG + chr3_1:70897043-70897062 None:intergenic 40.0%
AAGAAATGTAGGCGTGTGAT+GGG + chr3_1:70897062-70897081 None:intergenic 40.0%
AAAGAAATGTAGGCGTGTGA+TGG + chr3_1:70897063-70897082 None:intergenic 40.0%
GAGAAAGAAAGAAAGAAGGG+AGG + chr3_1:70897092-70897111 None:intergenic 40.0%
AACCTAAGAAGGAATGGAAG+TGG - chr3_1:70897388-70897407 Msa0372440:intron 40.0%
ACCTAAGAAGGAATGGAAGT+GGG - chr3_1:70897389-70897408 Msa0372440:intron 40.0%
TTGAGCCAACATGTTAGCAA+TGG + chr3_1:70897470-70897489 None:intergenic 40.0%
GAATGGAATCTAAGTGGGAA+AGG + chr3_1:70897534-70897553 None:intergenic 40.0%
GCGACAAAAACATACTGGAA+TGG + chr3_1:70897551-70897570 None:intergenic 40.0%
! ATGTGCAACATGGGCTATTA+TGG - chr3_1:70897962-70897981 Msa0372440:CDS 40.0%
CACAGAGACTCAAAATGTTG+AGG - chr3_1:70897985-70898004 Msa0372440:CDS 40.0%
TACATAGAAGCAGCCGTGTT+TGG - chr3_1:70896997-70897016 Msa0372440:CDS 45.0%
GTATGGGTGAGAACCTAAGA+AGG - chr3_1:70897377-70897396 Msa0372440:intron 45.0%
GGTGAGAACCTAAGAAGGAA+TGG - chr3_1:70897382-70897401 Msa0372440:intron 45.0%
TCCCACTTCCATTCCTTCTT+AGG + chr3_1:70897393-70897412 None:intergenic 45.0%
AGAAGTCATCAGCCATGTTG+TGG - chr3_1:70897915-70897934 Msa0372440:intron 45.0%
TCATCAGCCATGTTGTGGTT+GGG - chr3_1:70897920-70897939 Msa0372440:intron 45.0%
TACATAGAAGCAGCCGTGTT+TGG - chr3_1:70896997-70897016 Msa0372440:CDS 45.0%
GTATGGGTGAGAACCTAAGA+AGG - chr3_1:70897377-70897396 Msa0372440:intron 45.0%
GGTGAGAACCTAAGAAGGAA+TGG - chr3_1:70897382-70897401 Msa0372440:intron 45.0%
TCCCACTTCCATTCCTTCTT+AGG + chr3_1:70897393-70897412 None:intergenic 45.0%
AGAAGTCATCAGCCATGTTG+TGG - chr3_1:70897915-70897934 Msa0372440:intron 45.0%
TCATCAGCCATGTTGTGGTT+GGG - chr3_1:70897920-70897939 Msa0372440:intron 45.0%
!! ATACATAATAAATATAAATA+TGG + chr3_1:70897268-70897287 None:intergenic 5.0%
!! ATACATAATAAATATAAATA+TGG + chr3_1:70897268-70897287 None:intergenic 5.0%
TGGGAGTTCCAGCTCAAGTT+TGG - chr3_1:70897408-70897427 Msa0372440:intron 50.0%
GGGAGTTCCAGCTCAAGTTT+GGG - chr3_1:70897409-70897428 Msa0372440:intron 50.0%
TTTGGGCAGCAGCTTTCATG+AGG - chr3_1:70897426-70897445 Msa0372440:intron 50.0%
CAGCTTTCATGAGGACACAG+AGG - chr3_1:70897435-70897454 Msa0372440:intron 50.0%
CAAGACGAAACTCTTCACGC+CGG - chr3_1:70897487-70897506 Msa0372440:CDS 50.0%
GCTGATGACTTCTCCAAAGC+TGG + chr3_1:70897908-70897927 None:intergenic 50.0%
GTCATCAGCCATGTTGTGGT+TGG - chr3_1:70897919-70897938 Msa0372440:intron 50.0%
CCATGTTGTGGTTGGGATCA+GGG - chr3_1:70897927-70897946 Msa0372440:intron 50.0%
ATGTTGCACATGCAGATGGC+CGG + chr3_1:70897953-70897972 None:intergenic 50.0%
GCCATCTGCATGTGCAACAT+GGG - chr3_1:70897953-70897972 Msa0372440:CDS 50.0%
TGGGAGTTCCAGCTCAAGTT+TGG - chr3_1:70897408-70897427 Msa0372440:intron 50.0%
GGGAGTTCCAGCTCAAGTTT+GGG - chr3_1:70897409-70897428 Msa0372440:intron 50.0%
TTTGGGCAGCAGCTTTCATG+AGG - chr3_1:70897426-70897445 Msa0372440:intron 50.0%
CAGCTTTCATGAGGACACAG+AGG - chr3_1:70897435-70897454 Msa0372440:intron 50.0%
CAAGACGAAACTCTTCACGC+CGG - chr3_1:70897487-70897506 Msa0372440:CDS 50.0%
GCTGATGACTTCTCCAAAGC+TGG + chr3_1:70897908-70897927 None:intergenic 50.0%
GTCATCAGCCATGTTGTGGT+TGG - chr3_1:70897919-70897938 Msa0372440:intron 50.0%
CCATGTTGTGGTTGGGATCA+GGG - chr3_1:70897927-70897946 Msa0372440:intron 50.0%
ATGTTGCACATGCAGATGGC+CGG + chr3_1:70897953-70897972 None:intergenic 50.0%
GCCATCTGCATGTGCAACAT+GGG - chr3_1:70897953-70897972 Msa0372440:CDS 50.0%
TTTCCCGAGCTGTCTGCTAC+CGG + chr3_1:70897509-70897528 None:intergenic 55.0%
GCCATGTTGTGGTTGGGATC+AGG - chr3_1:70897926-70897945 Msa0372440:intron 55.0%
CCCTGATCCCAACCACAACA+TGG + chr3_1:70897930-70897949 None:intergenic 55.0%
GGCCATCTGCATGTGCAACA+TGG - chr3_1:70897952-70897971 Msa0372440:CDS 55.0%
GCCCATGTTGCACATGCAGA+TGG + chr3_1:70897957-70897976 None:intergenic 55.0%
TTTCCCGAGCTGTCTGCTAC+CGG + chr3_1:70897509-70897528 None:intergenic 55.0%
GCCATGTTGTGGTTGGGATC+AGG - chr3_1:70897926-70897945 Msa0372440:intron 55.0%
CCCTGATCCCAACCACAACA+TGG + chr3_1:70897930-70897949 None:intergenic 55.0%
GGCCATCTGCATGTGCAACA+TGG - chr3_1:70897952-70897971 Msa0372440:CDS 55.0%
GCCCATGTTGCACATGCAGA+TGG + chr3_1:70897957-70897976 None:intergenic 55.0%
GCTGCTGCCCAAACTTGAGC+TGG + chr3_1:70897419-70897438 None:intergenic 60.0%
GTTGTGGTTGGGATCAGGGC+CGG - chr3_1:70897931-70897950 Msa0372440:intron 60.0%
GCTGCTGCCCAAACTTGAGC+TGG + chr3_1:70897419-70897438 None:intergenic 60.0%
GTTGTGGTTGGGATCAGGGC+CGG - chr3_1:70897931-70897950 Msa0372440:intron 60.0%
CACGCCGGTAGCAGACAGCT+CGG - chr3_1:70897502-70897521 Msa0372440:CDS 65.0%
ACGCCGGTAGCAGACAGCTC+GGG - chr3_1:70897503-70897522 Msa0372440:CDS 65.0%
CACGCCGGTAGCAGACAGCT+CGG - chr3_1:70897502-70897521 Msa0372440:CDS 65.0%
ACGCCGGTAGCAGACAGCTC+GGG - chr3_1:70897503-70897522 Msa0372440:CDS 65.0%
Chromosome Type Strat End Strand Name
chr3_1 gene 70896955 70898027 70896955 ID=Msa0372440;Name=Msa0372440
chr3_1 mRNA 70896955 70898027 70896955 ID=Msa0372440-mRNA-1;Parent=Msa0372440;Name=Msa0372440-mRNA-1;_AED=0.61;_eAED=0.77;_QI=0|0|0|0.66|1|1|3|0|113
chr3_1 exon 70897936 70898027 70897936 ID=Msa0372440-mRNA-1:exon:15559;Parent=Msa0372440-mRNA-1
chr3_1 exon 70897474 70897604 70897474 ID=Msa0372440-mRNA-1:exon:15558;Parent=Msa0372440-mRNA-1
chr3_1 exon 70896955 70897073 70896955 ID=Msa0372440-mRNA-1:exon:15557;Parent=Msa0372440-mRNA-1
chr3_1 CDS 70897936 70898027 70897936 ID=Msa0372440-mRNA-1:cds;Parent=Msa0372440-mRNA-1
chr3_1 CDS 70897474 70897604 70897474 ID=Msa0372440-mRNA-1:cds;Parent=Msa0372440-mRNA-1
chr3_1 CDS 70896955 70897073 70896955 ID=Msa0372440-mRNA-1:cds;Parent=Msa0372440-mRNA-1
Gene Sequence

>Msa0372440

ATGAAAATAAGACTCAACAGTGGTCAATTACGTAACAATTGTTACATAGAAGCAGCCGTGTTTGGTTTTTCATTTCTTCCAGAAGCCAAACTTATGGGTGAGAACCTAAGAAGGAATGGAAGTGGGAGTTCCAGCTCAAGTTTGGGCAGCAGCTTTCATGAGGACACAGAGGATGATCAAACCATTGCTAACATGTTGGCTCAAGACGAAACTCTTCACGCCGCTTTGGAGAAGTCATCAGCCATGTTGTGGTTGGGATCAGGGCCGGCCATCTGCATGTGCAACATGGGCTATTATGGCACAGAGACTCAAAATGTTGAGGGTCTAAATCCCAAATTTTGA

Protein sequence

>Msa0372440

MKIRLNSGQLRNNCYIEAAVFGFSFLPEAKLMGENLRRNGSGSSSSSLGSSFHEDTEDDQTIANMLAQDETLHAALEKSSAMLWLGSGPAICMCNMGYYGTETQNVEGLNPKF*