AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0459960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0459960 MtrunA17_Chr3g0108671 95.833 216 9 0 1 216 1 216 2.86e-155 428
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0459960 AT5G58250.1 77.698 139 31 0 75 213 70 208 4.05e-77 231

Find 80 sgRNAs with CRISPR-Local

Find 237 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACCACCCAGATTCATATTT+AGG 0.214169 3_3:+56955588 None:intergenic
TTATTGATTGCCATATAAAA+AGG 0.239359 3_3:-56955134 None:intergenic
CCCTATCCTAAATATGAATC+TGG 0.285716 3_3:-56955594 Msa0459960:CDS
GACGTCAAAGATGAGATATT+TGG 0.293677 3_3:-56955225 Msa0459960:three_prime_UTR
TGTCATTGAGCCTAAGTTCT+TGG 0.311126 3_3:-56956412 Msa0459960:CDS
TTTGAACGCCGTCGCTACTA+TGG 0.311898 3_3:-56956465 Msa0459960:CDS
CCCCATTCTGTTTCAGTAAC+CGG 0.313035 3_3:-56955509 Msa0459960:three_prime_UTR
GAAGCAACAGCAGTGATAAA+AGG 0.313085 3_3:+56957955 None:intergenic
AAGAGAGAGTGGGACAATTC+GGG 0.320498 3_3:+56958268 None:intergenic
GTTAGAAAGGATGTGCCTTT+TGG 0.329162 3_3:+56958041 None:intergenic
GGCGTTCAAATAGTTGTTCT+TGG 0.330758 3_3:+56956478 None:intergenic
AAAGAGAGAGTGGGACAATT+CGG 0.331588 3_3:+56958267 None:intergenic
TCACTCTTTACCAATGAGTT+TGG 0.337284 3_3:-56958013 Msa0459960:CDS
AGAAATAGATCTACAAATTC+CGG 0.337387 3_3:+56955490 None:intergenic
GTCCTTAGGCTCTAAGTTCT+AGG 0.343635 3_3:-56955360 Msa0459960:three_prime_UTR
GTAATAAAGAACAGGATTAC+TGG 0.361902 3_3:-56956437 Msa0459960:CDS
CGCAGTTTCTTGATAAAGTC+AGG 0.393349 3_3:+56956381 None:intergenic
ATCTATATTTCACTCATTTG+AGG 0.401260 3_3:+56955324 None:intergenic
TATAAAGTTTGTGTAAAGGC+TGG 0.408888 3_3:-56955198 Msa0459960:three_prime_UTR
TGGGAAACTTTCTTGCCCTC+TGG 0.417486 3_3:-56955567 Msa0459960:CDS
TTCTCAAATTCCAGCTTAGT+AGG 0.423773 3_3:+56955636 None:intergenic
AAATTCCAGCTTAGTAGGAT+TGG 0.428131 3_3:+56955641 None:intergenic
AAAGTTGAAGTGTTGTCCTT+AGG 0.436163 3_3:-56955374 Msa0459960:three_prime_UTR
AACAAGAGCTAGCACGATGC+TGG 0.441102 3_3:-56958127 Msa0459960:exon
TCCGGTTACTGAAACAGAAT+GGG 0.442631 3_3:+56955508 None:intergenic
AATTTGAGAAGCCTGCAAAA+TGG 0.444037 3_3:-56955623 Msa0459960:CDS
TGCAAATGCAAAATTTATGC+TGG 0.444095 3_3:-56956517 Msa0459960:CDS
AAGAGTGAGTTCTGTTAGAA+AGG 0.452390 3_3:+56958028 None:intergenic
GTTTGAGCTACCATTGTAGT+TGG 0.453138 3_3:+56958078 None:intergenic
CCAGATTCATATTTAGGATA+GGG 0.459432 3_3:+56955594 None:intergenic
TAGTGTCAACCAACGGTCCT+TGG 0.460321 3_3:-56956341 Msa0459960:CDS
CTTGCCCTCTGGACAGAAAG+AGG 0.461986 3_3:-56955556 Msa0459960:CDS
GGCAATTGGCAAAGAGAGAG+TGG 0.462145 3_3:+56958257 None:intergenic
TGCAACAATAAAATAATACT+TGG 0.464259 3_3:+56956536 None:intergenic
AGCTAGCACGATGCTGGGCA+TGG 0.469448 3_3:-56958121 Msa0459960:exon
CAGATTCATGAAATTAAGAC+TGG 0.474576 3_3:-56955718 Msa0459960:CDS
GAGTGGAGAAAGATGGCAAT+TGG 0.484031 3_3:+56958243 None:intergenic
TGGAGAACGTAATAAAGAAC+AGG 0.488529 3_3:-56956445 Msa0459960:CDS
TGCAAAATTTATGCTGGATG+AGG 0.488625 3_3:-56956511 Msa0459960:CDS
ATTATATAAAGTTTGTGTAA+AGG 0.494941 3_3:-56955202 Msa0459960:three_prime_UTR
TTCCGGTTACTGAAACAGAA+TGG 0.495540 3_3:+56955507 None:intergenic
GTAGGAGAGACCAAACTCAT+TGG 0.500788 3_3:+56958003 None:intergenic
ATTTGAGAAGCCTGCAAAAT+GGG 0.501452 3_3:-56955622 Msa0459960:CDS
AGTTGGTGAACTCAAAGTTA+AGG 0.501880 3_3:+56958095 None:intergenic
TTCCAGCTTAGTAGGATTGG+AGG 0.503729 3_3:+56955644 None:intergenic
CCCAGATTCATATTTAGGAT+AGG 0.506511 3_3:+56955593 None:intergenic
GCTAGCACGATGCTGGGCAT+GGG 0.507041 3_3:-56958120 Msa0459960:exon
CTAAATATGAATCTGGGTGG+TGG 0.508070 3_3:-56955587 Msa0459960:CDS
AGCCTCCAATCCTACTAAGC+TGG 0.514208 3_3:-56955646 Msa0459960:CDS
TAAATATGAATCTGGGTGGT+GGG 0.516208 3_3:-56955586 Msa0459960:CDS
TACCTAGAACTTAGAGCCTA+AGG 0.520854 3_3:+56955358 None:intergenic
CTACTCTTACCTCTTCCAAA+AGG 0.529128 3_3:-56958056 Msa0459960:CDS
GGAAAACTATCCAAGAACTT+AGG 0.529282 3_3:+56956402 None:intergenic
TTCTGATGCACCTGCCACCA+AGG 0.538067 3_3:-56957908 Msa0459960:intron
AGGAGATAACTAGATAGATA+AGG 0.549778 3_3:-56958306 Msa0459960:five_prime_UTR
CCTATCCTAAATATGAATCT+GGG 0.552298 3_3:-56955593 Msa0459960:CDS
TTATGTTCTTACGTGATCCA+AGG 0.559994 3_3:+56956324 None:intergenic
TTCACCTCTTTCTGTCCAGA+GGG 0.560963 3_3:+56955552 None:intergenic
CTAGCACGATGCTGGGCATG+GGG 0.565374 3_3:-56958119 Msa0459960:exon
AAAATTTATGCTGGATGAGG+AGG 0.565968 3_3:-56956508 Msa0459960:CDS
ACAAGAGCTAGCACGATGCT+GGG 0.567957 3_3:-56958126 Msa0459960:exon
CAGCAAAGAGTGGAGAAAGA+TGG 0.569234 3_3:+56958236 None:intergenic
TTACGTGATCCAAGGACCGT+TGG 0.571710 3_3:+56956332 None:intergenic
CACAATATCTAACACGCAGT+CGG 0.588526 3_3:+56955263 None:intergenic
GTTCACCTCTTTCTGTCCAG+AGG 0.589301 3_3:+56955551 None:intergenic
GTTATGACAACAGCAAAGAG+TGG 0.590132 3_3:+56958226 None:intergenic
TACGTTCTCCATAGTAGCGA+CGG 0.602345 3_3:+56956457 None:intergenic
AGAGAGAGTGGGACAATTCG+GGG 0.612656 3_3:+56958269 None:intergenic
TTTGAGTTCACCAACTACAA+TGG 0.618572 3_3:-56958088 Msa0459960:CDS
GCAATTGGCAAAGAGAGAGT+GGG 0.634379 3_3:+56958258 None:intergenic
TGAGAAGCCTGCAAAATGGG+TGG 0.654553 3_3:-56955619 Msa0459960:CDS
CTGACTTTATCAAGAAACTG+CGG 0.655587 3_3:-56956380 Msa0459960:CDS
GTTGACACTAGAGCAACAGC+AGG 0.658553 3_3:+56956354 None:intergenic
TGTGATAAGATAACGCACAA+AGG 0.658969 3_3:+56958155 None:intergenic
CCGGTTACTGAAACAGAATG+GGG 0.659040 3_3:+56955509 None:intergenic
GTTGCTCTAGTGTCAACCAA+CGG 0.671470 3_3:-56956348 Msa0459960:CDS
GGTTACTGAAACAGAATGGG+GGG 0.677815 3_3:+56955511 None:intergenic
GAGAGAGTGGGACAATTCGG+GGG 0.678722 3_3:+56958270 None:intergenic
CGGTTACTGAAACAGAATGG+GGG 0.679526 3_3:+56955510 None:intergenic
ATCCTAAATATGAATCTGGG+TGG 0.714399 3_3:-56955590 Msa0459960:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGATTTTTTTTTTTTTTTGA+AGG - chr3_3:56955144-56955163 Msa0459960:three_prime_UTR 10.0%
!! TAATGTCTTTAAATTAAAAA+AGG + chr3_3:56956132-56956151 None:intergenic 10.0%
!!! TATTTTATTTTATTTTTGTG+GGG - chr3_3:56957237-56957256 Msa0459960:intron 10.0%
!!! ATATTAAATTACTTTTTTGA+GGG + chr3_3:56957342-56957361 None:intergenic 10.0%
!!! TATTAAATTACTTTTTTGAG+GGG + chr3_3:56957341-56957360 None:intergenic 15.0%
!!! GATATTAAATTACTTTTTTG+AGG + chr3_3:56957343-56957362 None:intergenic 15.0%
!! AAAAGAAATTGTAAGAAATA+AGG - chr3_3:56957625-56957644 Msa0459960:intron 15.0%
!! AAAGAAATTGTAAGAAATAA+GGG - chr3_3:56957626-56957645 Msa0459960:intron 15.0%
!! TTTACATTAATAAAAATCGT+TGG + chr3_3:56958006-56958025 None:intergenic 15.0%
!!! GATTTTTATTAATGTAAAAC+TGG - chr3_3:56958008-56958027 Msa0459960:CDS 15.0%
!! ATTATATAAAGTTTGTGTAA+AGG - chr3_3:56958268-56958287 Msa0459960:five_prime_UTR 15.0%
!!! GTTTTTGTTTCTTGAAATTT+TGG + chr3_3:56955496-56955515 None:intergenic 20.0%
!!! AATATTTGTTTTCTACTAAG+TGG - chr3_3:56955642-56955661 Msa0459960:CDS 20.0%
!! TAAATGTTTGAATGTTTGAA+AGG - chr3_3:56955708-56955727 Msa0459960:CDS 20.0%
!! AAATGTTTGAATGTTTGAAA+GGG - chr3_3:56955709-56955728 Msa0459960:CDS 20.0%
!!! AATTTTATGGACTAATTACA+AGG + chr3_3:56955770-56955789 None:intergenic 20.0%
!!! ATCAAGGATTTTCAATTTTA+TGG + chr3_3:56955783-56955802 None:intergenic 20.0%
!! TGCAACAATAAAATAATACT+TGG + chr3_3:56956937-56956956 None:intergenic 20.0%
!!! ATTAAATTACTTTTTTGAGG+GGG + chr3_3:56957340-56957359 None:intergenic 20.0%
!! TCATATTATACCATTTGAAA+CGG + chr3_3:56957606-56957625 None:intergenic 20.0%
!!! TTCTTGAAATTTTGGTTTGT+AGG + chr3_3:56955488-56955507 None:intergenic 25.0%
! ACAAGACATAGTTATAATGT+GGG + chr3_3:56955618-56955637 None:intergenic 25.0%
!! AATTTAAAATCTCAACCGTT+AGG - chr3_3:56956017-56956036 Msa0459960:intron 25.0%
! AGTTCCATTTACATCATTTA+GGG + chr3_3:56956319-56956338 None:intergenic 25.0%
! AAGTTCCATTTACATCATTT+AGG + chr3_3:56956320-56956339 None:intergenic 25.0%
! TCATTCTCTCTTATCTTTAA+AGG + chr3_3:56956513-56956532 None:intergenic 25.0%
! TTAAAGATAAGAGAGAATGA+TGG - chr3_3:56956513-56956532 Msa0459960:CDS 25.0%
! AGTTCATTTACGTATCAAAT+TGG - chr3_3:56956735-56956754 Msa0459960:intron 25.0%
!!! ACTTATTGAGTTGGTTTTTA+TGG - chr3_3:56956864-56956883 Msa0459960:intron 25.0%
!! CACTTTTAATATCTGATATG+TGG + chr3_3:56957381-56957400 None:intergenic 25.0%
! CACATATCAGATATTAAAAG+TGG - chr3_3:56957379-56957398 Msa0459960:intron 25.0%
! TTAAAAGTGGACAATTCATT+TGG - chr3_3:56957392-56957411 Msa0459960:intron 25.0%
! TGACAAACATAGAATACAAA+CGG + chr3_3:56957479-56957498 None:intergenic 25.0%
! AGAAATAGATCTACAAATTC+CGG + chr3_3:56957983-56958002 None:intergenic 25.0%
!!! GCTTTCAGTTTTGTAATTTT+TGG - chr3_3:56958055-56958074 Msa0459960:CDS 25.0%
!!! TCAGTTTTGTAATTTTTGGA+AGG - chr3_3:56958059-56958078 Msa0459960:CDS 25.0%
! ATCTATATTTCACTCATTTG+AGG + chr3_3:56958149-56958168 None:intergenic 25.0%
AGGAGATAACTAGATAGATA+AGG - chr3_3:56955164-56955183 Msa0459960:three_prime_UTR 30.0%
GATAAGAGAAAAAGAAACCT+TGG + chr3_3:56955582-56955601 None:intergenic 30.0%
CACAAGACATAGTTATAATG+TGG + chr3_3:56955619-56955638 None:intergenic 30.0%
!!! TGCAATTTTGAGGTCAATTT+TGG + chr3_3:56955746-56955765 None:intergenic 30.0%
! ATTACAAGGATGCAATTTTG+AGG + chr3_3:56955756-56955775 None:intergenic 30.0%
GACTCAAAATAATGTGATCA+AGG + chr3_3:56955799-56955818 None:intergenic 30.0%
TGCGGCAAAAAAATATCTAA+CGG - chr3_3:56955883-56955902 Msa0459960:intron 30.0%
TAAATGGAACTTAGCCTAAA+TGG - chr3_3:56956328-56956347 Msa0459960:intron 30.0%
! AGAACTACTAGTTTCATTTC+AGG - chr3_3:56956758-56956777 Msa0459960:intron 30.0%
TTTCATTTCAGGAACTGAAT+TGG - chr3_3:56956769-56956788 Msa0459960:intron 30.0%
! TCTTGATGGTTTATAAGCAT+TGG - chr3_3:56956830-56956849 Msa0459960:intron 30.0%
TGCAAATGCAAAATTTATGC+TGG - chr3_3:56956953-56956972 Msa0459960:intron 30.0%
GTAATAAAGAACAGGATTAC+TGG - chr3_3:56957033-56957052 Msa0459960:intron 30.0%
!! ATACTTCATTTTTCAGTGCT+TGG - chr3_3:56957173-56957192 Msa0459960:intron 30.0%
CTGTCATAAACTATGAAAAG+TGG - chr3_3:56957201-56957220 Msa0459960:intron 30.0%
! TATTATACCATTTGAAACGG+TGG + chr3_3:56957603-56957622 None:intergenic 30.0%
GCTGTCAACACATAATAAAA+TGG - chr3_3:56957677-56957696 Msa0459960:intron 30.0%
CAGATTCATGAAATTAAGAC+TGG - chr3_3:56957752-56957771 Msa0459960:intron 30.0%
! CCAGATTCATATTTAGGATA+GGG + chr3_3:56957879-56957898 None:intergenic 30.0%
CCTATCCTAAATATGAATCT+GGG - chr3_3:56957877-56957896 Msa0459960:intron 30.0%
TATAAAGTTTGTGTAAAGGC+TGG - chr3_3:56958272-56958291 Msa0459960:five_prime_UTR 30.0%
TGTGATAAGATAACGCACAA+AGG + chr3_3:56955318-56955337 None:intergenic 35.0%
!! AGTTGGTGAACTCAAAGTTA+AGG + chr3_3:56955378-56955397 None:intergenic 35.0%
! TTTGAGTTCACCAACTACAA+TGG - chr3_3:56955382-56955401 Msa0459960:three_prime_UTR 35.0%
AAGAGTGAGTTCTGTTAGAA+AGG + chr3_3:56955445-56955464 None:intergenic 35.0%
TCACTCTTTACCAATGAGTT+TGG - chr3_3:56955457-56955476 Msa0459960:three_prime_UTR 35.0%
AAGAGAAAAAGAAACCTTGG+TGG + chr3_3:56955579-56955598 None:intergenic 35.0%
TTGAGTCAGTTTAGTGAGTT+CGG - chr3_3:56955812-56955831 Msa0459960:intron 35.0%
TAAATGGAGCTTAGCCTAAA+TGG + chr3_3:56955845-56955864 None:intergenic 35.0%
AAGCTCCATTTACTCCATTT+AGG - chr3_3:56955853-56955872 Msa0459960:intron 35.0%
AGCTCCATTTACTCCATTTA+GGG - chr3_3:56955854-56955873 Msa0459960:intron 35.0%
CAAACCCTAAATGGAGTAAA+TGG + chr3_3:56955861-56955880 None:intergenic 35.0%
! CATTTACTCCATTTAGGGTT+TGG - chr3_3:56955859-56955878 Msa0459960:intron 35.0%
TGTTGTGATATGAGAGAAGA+AGG - chr3_3:56955938-56955957 Msa0459960:intron 35.0%
GTTGTGATATGAGAGAAGAA+GGG - chr3_3:56955939-56955958 Msa0459960:intron 35.0%
!!! CTCAACCGTTAGGTATTTTT+TGG - chr3_3:56956027-56956046 Msa0459960:intron 35.0%
TGTGGCCAAAAAATACCTAA+CGG + chr3_3:56956035-56956054 None:intergenic 35.0%
! ACATCATTTAGGGTGTGTTT+GGG + chr3_3:56956309-56956328 None:intergenic 35.0%
! TACATCATTTAGGGTGTGTT+TGG + chr3_3:56956310-56956329 None:intergenic 35.0%
CACACCCTAAATGATGTAAA+TGG - chr3_3:56956312-56956331 Msa0459960:intron 35.0%
TTAGGTACTAAACTGGAGTT+CGG + chr3_3:56956361-56956380 None:intergenic 35.0%
!! ATGACACCTTCTAGTTCATA+AGG + chr3_3:56956478-56956497 None:intergenic 35.0%
!! TATGAACTAGAAGGTGTCAT+TGG - chr3_3:56956478-56956497 Msa0459960:CDS 35.0%
!!! TAGAAGGTGTCATTGGTTTT+TGG - chr3_3:56956485-56956504 Msa0459960:CDS 35.0%
TGTAGATGAGAGAGAATGAT+TGG - chr3_3:56956557-56956576 Msa0459960:intron 35.0%
! TGATTGGTTAAACGAGAGAT+AGG - chr3_3:56956573-56956592 Msa0459960:intron 35.0%
!! TTTTGTTTAAACCCTCCAGT+AGG + chr3_3:56956637-56956656 None:intergenic 35.0%
GTCAATGTCAGATCACTTTA+GGG + chr3_3:56956670-56956689 None:intergenic 35.0%
AGTCAATGTCAGATCACTTT+AGG + chr3_3:56956671-56956690 None:intergenic 35.0%
CAATTAGCTGTTGTTCTTGA+TGG - chr3_3:56956816-56956835 Msa0459960:intron 35.0%
TCACGTAGTACTTATTGAGT+TGG - chr3_3:56956855-56956874 Msa0459960:intron 35.0%
TGCAAAATTTATGCTGGATG+AGG - chr3_3:56956959-56956978 Msa0459960:intron 35.0%
AAAATTTATGCTGGATGAGG+AGG - chr3_3:56956962-56956981 Msa0459960:intron 35.0%
TGGAGAACGTAATAAAGAAC+AGG - chr3_3:56957025-56957044 Msa0459960:intron 35.0%
GGAAAACTATCCAAGAACTT+AGG + chr3_3:56957071-56957090 None:intergenic 35.0%
CTGACTTTATCAAGAAACTG+CGG - chr3_3:56957090-56957109 Msa0459960:intron 35.0%
TTATGTTCTTACGTGATCCA+AGG + chr3_3:56957149-56957168 None:intergenic 35.0%
!! CACTTCAATAGCTTGCTATT+GGG + chr3_3:56957311-56957330 None:intergenic 35.0%
TTAATATCGTGTGACCCATT+AGG - chr3_3:56957355-56957374 Msa0459960:intron 35.0%
TATCTGATATGTGGCCTAAT+GGG + chr3_3:56957372-56957391 None:intergenic 35.0%
ATATCTGATATGTGGCCTAA+TGG + chr3_3:56957373-56957392 None:intergenic 35.0%
AATCAGCATATCTCAATCGT+TGG - chr3_3:56957441-56957460 Msa0459960:intron 35.0%
!!! AAATTTTAACTCTTCCCCTG+TGG - chr3_3:56957720-56957739 Msa0459960:intron 35.0%
!!! AATTTTAACTCTTCCCCTGT+GGG - chr3_3:56957721-56957740 Msa0459960:intron 35.0%
AAATTCCAGCTTAGTAGGAT+TGG + chr3_3:56957832-56957851 None:intergenic 35.0%
TTCTCAAATTCCAGCTTAGT+AGG + chr3_3:56957837-56957856 None:intergenic 35.0%
AATTTGAGAAGCCTGCAAAA+TGG - chr3_3:56957847-56957866 Msa0459960:intron 35.0%
ATTTGAGAAGCCTGCAAAAT+GGG - chr3_3:56957848-56957867 Msa0459960:intron 35.0%
CCCTATCCTAAATATGAATC+TGG - chr3_3:56957876-56957895 Msa0459960:intron 35.0%
! CCCAGATTCATATTTAGGAT+AGG + chr3_3:56957880-56957899 None:intergenic 35.0%
ATCCTAAATATGAATCTGGG+TGG - chr3_3:56957880-56957899 Msa0459960:intron 35.0%
TAAATATGAATCTGGGTGGT+GGG - chr3_3:56957884-56957903 Msa0459960:intron 35.0%
!! AAAGTTGAAGTGTTGTCCTT+AGG - chr3_3:56958096-56958115 Msa0459960:CDS 35.0%
GACGTCAAAGATGAGATATT+TGG - chr3_3:56958245-56958264 Msa0459960:five_prime_UTR 35.0%
AAAGAGAGAGTGGGACAATT+CGG + chr3_3:56955206-56955225 None:intergenic 40.0%
GTTATGACAACAGCAAAGAG+TGG + chr3_3:56955247-56955266 None:intergenic 40.0%
GTTTGAGCTACCATTGTAGT+TGG + chr3_3:56955395-56955414 None:intergenic 40.0%
CTACTCTTACCTCTTCCAAA+AGG - chr3_3:56955414-56955433 Msa0459960:three_prime_UTR 40.0%
! GTTAGAAAGGATGTGCCTTT+TGG + chr3_3:56955432-56955451 None:intergenic 40.0%
GAAGCAACAGCAGTGATAAA+AGG + chr3_3:56955518-56955537 None:intergenic 40.0%
TGAGTCAGTTTAGTGAGTTC+GGG - chr3_3:56955813-56955832 Msa0459960:intron 40.0%
TAGGTACTAAACTGGAGTTC+GGG + chr3_3:56956360-56956379 None:intergenic 40.0%
CTCCAGTTTAGTACCTAAAG+TGG - chr3_3:56956363-56956382 Msa0459960:CDS 40.0%
GACCACTTTAGGTACTAAAC+TGG + chr3_3:56956368-56956387 None:intergenic 40.0%
GTTCTCTTGAAGACCACTTT+AGG + chr3_3:56956379-56956398 None:intergenic 40.0%
! GACGAAAGATTGTTCTAGCT+AGG + chr3_3:56956414-56956433 None:intergenic 40.0%
! TCACAATCTAGGATTCGACA+GGG + chr3_3:56956452-56956471 None:intergenic 40.0%
! TTCACAATCTAGGATTCGAC+AGG + chr3_3:56956453-56956472 None:intergenic 40.0%
TGTCGAATCCTAGATTGTGA+AGG - chr3_3:56956452-56956471 Msa0459960:CDS 40.0%
TCATAAGGCCTTCACAATCT+AGG + chr3_3:56956463-56956482 None:intergenic 40.0%
! TGAAGGCCTTATGAACTAGA+AGG - chr3_3:56956469-56956488 Msa0459960:CDS 40.0%
GATAGGAACCAATGTACCTA+AGG - chr3_3:56956590-56956609 Msa0459960:intron 40.0%
GGCGTTCAAATAGTTGTTCT+TGG + chr3_3:56956995-56957014 None:intergenic 40.0%
TGTCATTGAGCCTAAGTTCT+TGG - chr3_3:56957058-56957077 Msa0459960:intron 40.0%
CGCAGTTTCTTGATAAAGTC+AGG + chr3_3:56957092-56957111 None:intergenic 40.0%
GTTGCACTATTGTATGAGCT+TGG + chr3_3:56957267-56957286 None:intergenic 40.0%
!! CCACTTCAATAGCTTGCTAT+TGG + chr3_3:56957312-56957331 None:intergenic 40.0%
CCAATAGCAAGCTATTGAAG+TGG - chr3_3:56957309-56957328 Msa0459960:intron 40.0%
!!! ATTACTTTTTTGAGGGGGAG+AGG + chr3_3:56957335-56957354 None:intergenic 40.0%
TTAGCAACTGACACCTCTAA+GGG + chr3_3:56957511-56957530 None:intergenic 40.0%
TTTAGCAACTGACACCTCTA+AGG + chr3_3:56957512-56957531 None:intergenic 40.0%
TAGAGATCCACCGTTTCAAA+TGG - chr3_3:56957593-56957612 Msa0459960:intron 40.0%
!! AGACTGGATCGAGTTTTATC+AGG - chr3_3:56957768-56957787 Msa0459960:intron 40.0%
CACCACCCAGATTCATATTT+AGG + chr3_3:56957885-56957904 None:intergenic 40.0%
CTAAATATGAATCTGGGTGG+TGG - chr3_3:56957883-56957902 Msa0459960:intron 40.0%
TCCGGTTACTGAAACAGAAT+GGG + chr3_3:56957965-56957984 None:intergenic 40.0%
TTCCGGTTACTGAAACAGAA+TGG + chr3_3:56957966-56957985 None:intergenic 40.0%
TACCTAGAACTTAGAGCCTA+AGG + chr3_3:56958115-56958134 None:intergenic 40.0%
CACAATATCTAACACGCAGT+CGG + chr3_3:56958210-56958229 None:intergenic 40.0%
AAGAGAGAGTGGGACAATTC+GGG + chr3_3:56955205-56955224 None:intergenic 45.0%
GCAATTGGCAAAGAGAGAGT+GGG + chr3_3:56955215-56955234 None:intergenic 45.0%
GAGTGGAGAAAGATGGCAAT+TGG + chr3_3:56955230-56955249 None:intergenic 45.0%
CAGCAAAGAGTGGAGAAAGA+TGG + chr3_3:56955237-56955256 None:intergenic 45.0%
!! AAGGATGTGCCTTTTGGAAG+AGG + chr3_3:56955426-56955445 None:intergenic 45.0%
GTAGGAGAGACCAAACTCAT+TGG + chr3_3:56955470-56955489 None:intergenic 45.0%
! GAAAAAGAAACCTTGGTGGC+AGG + chr3_3:56955575-56955594 None:intergenic 45.0%
AGTTCGGGTATGCTCCATTT+AGG - chr3_3:56955828-56955847 Msa0459960:intron 45.0%
! CTCCATTTAGGGTTTGGATG+CGG - chr3_3:56955865-56955884 Msa0459960:intron 45.0%
TGTCTTGCATCCAAACCCTA+AGG - chr3_3:56956055-56956074 Msa0459960:intron 45.0%
GTCTTGCATCCAAACCCTAA+GGG - chr3_3:56956056-56956075 Msa0459960:intron 45.0%
! CAAACCCTAAGGGTGTGTTT+GGG - chr3_3:56956066-56956085 Msa0459960:intron 45.0%
TCTTCCCAAACACACCCTTA+GGG + chr3_3:56956073-56956092 None:intergenic 45.0%
! TAAGGGTGTGTTTGGGAAGA+GGG - chr3_3:56956073-56956092 Msa0459960:intron 45.0%
TGTGTTTGGGAAGAGGGTTT+AGG - chr3_3:56956079-56956098 Msa0459960:intron 45.0%
TTTGGGAAGAGGGTTTAGGA+GGG - chr3_3:56956083-56956102 Msa0459960:intron 45.0%
! TCATTTAGGGTGTGTTTGGG+AGG + chr3_3:56956306-56956325 None:intergenic 45.0%
AGTTCGGGTATGCTCCATTT+AGG + chr3_3:56955828-56955847 None:intergenic 45.0%
ACCAATGTACCTAAGGCCAA+TGG - chr3_3:56956597-56956616 Msa0459960:intron 45.0%
GGATTTCACTTGGATGCCAT+TGG + chr3_3:56956616-56956635 None:intergenic 45.0%
GCATCCAAGTGAAATCCTAC+TGG - chr3_3:56956619-56956638 Msa0459960:intron 45.0%
TCCAAGTGAAATCCTACTGG+AGG - chr3_3:56956622-56956641 Msa0459960:intron 45.0%
CCAAGTGAAATCCTACTGGA+GGG - chr3_3:56956623-56956642 Msa0459960:intron 45.0%
TACGTTCTCCATAGTAGCGA+CGG + chr3_3:56957016-56957035 None:intergenic 45.0%
GTTGCTCTAGTGTCAACCAA+CGG - chr3_3:56957122-56957141 Msa0459960:intron 45.0%
TAGCAACTGACACCTCTAAG+GGG + chr3_3:56957510-56957529 None:intergenic 45.0%
AACGGTGGATCTCTATCTGA+TGG + chr3_3:56957588-56957607 None:intergenic 45.0%
AATCTGCCAAAACACCCACA+GGG + chr3_3:56957738-56957757 None:intergenic 45.0%
TTCCAGCTTAGTAGGATTGG+AGG + chr3_3:56957829-56957848 None:intergenic 45.0%
TTCACCTCTTTCTGTCCAGA+GGG + chr3_3:56957921-56957940 None:intergenic 45.0%
GGTTACTGAAACAGAATGGG+GGG + chr3_3:56957962-56957981 None:intergenic 45.0%
CGGTTACTGAAACAGAATGG+GGG + chr3_3:56957963-56957982 None:intergenic 45.0%
CCGGTTACTGAAACAGAATG+GGG + chr3_3:56957964-56957983 None:intergenic 45.0%
CCCCATTCTGTTTCAGTAAC+CGG - chr3_3:56957961-56957980 Msa0459960:CDS 45.0%
!! GTCCTTAGGCTCTAAGTTCT+AGG - chr3_3:56958110-56958129 Msa0459960:CDS 45.0%
!!! TTTATTTTATTTTATTTTTG+TGG - chr3_3:56957235-56957254 Msa0459960:intron 5.0%
!!! TTATTTTATTTTATTTTTGT+GGG - chr3_3:56957236-56957255 Msa0459960:intron 5.0%
GGACAATTCGGGGGATATCA+TGG + chr3_3:56955194-56955213 None:intergenic 50.0%
AGAGAGAGTGGGACAATTCG+GGG + chr3_3:56955204-56955223 None:intergenic 50.0%
GGCAATTGGCAAAGAGAGAG+TGG + chr3_3:56955216-56955235 None:intergenic 50.0%
AACAAGAGCTAGCACGATGC+TGG - chr3_3:56955343-56955362 Msa0459960:three_prime_UTR 50.0%
ACAAGAGCTAGCACGATGCT+GGG - chr3_3:56955344-56955363 Msa0459960:three_prime_UTR 50.0%
TGCCGCATCCAAACCCTAAA+TGG + chr3_3:56955870-56955889 None:intergenic 50.0%
GGGTTTGGATGCAAGACATG+TGG + chr3_3:56956053-56956072 None:intergenic 50.0%
CCAAACACACCCTTAGGGTT+TGG + chr3_3:56956068-56956087 None:intergenic 50.0%
! CCAAACCCTAAGGGTGTGTT+TGG - chr3_3:56956065-56956084 Msa0459960:intron 50.0%
CTCTTCCCAAACACACCCTT+AGG + chr3_3:56956074-56956093 None:intergenic 50.0%
! CTAAGGGTGTGTTTGGGAAG+AGG - chr3_3:56956072-56956091 Msa0459960:intron 50.0%
GTTTGGGAAGAGGGTTTAGG+AGG - chr3_3:56956082-56956101 Msa0459960:intron 50.0%
TTGGGAAGAGGGTTTAGGAG+GGG - chr3_3:56956084-56956103 Msa0459960:intron 50.0%
AAGAGGGTTTAGGAGGGGAA+GGG - chr3_3:56956089-56956108 Msa0459960:intron 50.0%
TTTGGGAGGAGGGTTTAGGA+GGG + chr3_3:56956292-56956311 None:intergenic 50.0%
TGTGTTTGGGAGGAGGGTTT+AGG + chr3_3:56956296-56956315 None:intergenic 50.0%
TTAGGGTGTGTTTGGGAGGA+GGG + chr3_3:56956302-56956321 None:intergenic 50.0%
! TTTAGGGTGTGTTTGGGAGG+AGG + chr3_3:56956303-56956322 None:intergenic 50.0%
!! GCCATTGGCCTTAGGTACAT+TGG + chr3_3:56956601-56956620 None:intergenic 50.0%
!! ACTTGGATGCCATTGGCCTT+AGG + chr3_3:56956609-56956628 None:intergenic 50.0%
CCCTCCAGTAGGATTTCACT+TGG + chr3_3:56956626-56956645 None:intergenic 50.0%
TTTGAACGCCGTCGCTACTA+TGG - chr3_3:56957005-56957024 Msa0459960:intron 50.0%
GTTGACACTAGAGCAACAGC+AGG + chr3_3:56957119-56957138 None:intergenic 50.0%
TAGTGTCAACCAACGGTCCT+TGG - chr3_3:56957129-56957148 Msa0459960:intron 50.0%
TTACGTGATCCAAGGACCGT+TGG + chr3_3:56957141-56957160 None:intergenic 50.0%
! GTCAGCGTTTTACCCCTTAG+AGG - chr3_3:56957495-56957514 Msa0459960:intron 50.0%
ATCTGCCAAAACACCCACAG+GGG + chr3_3:56957737-56957756 None:intergenic 50.0%
GAATCTGCCAAAACACCCAC+AGG + chr3_3:56957739-56957758 None:intergenic 50.0%
AGCCTCCAATCCTACTAAGC+TGG - chr3_3:56957824-56957843 Msa0459960:intron 50.0%
TGAGAAGCCTGCAAAATGGG+TGG - chr3_3:56957851-56957870 Msa0459960:intron 50.0%
TGGGAAACTTTCTTGCCCTC+TGG - chr3_3:56957903-56957922 Msa0459960:intron 50.0%
GTTCACCTCTTTCTGTCCAG+AGG + chr3_3:56957922-56957941 None:intergenic 50.0%
GAGAGAGTGGGACAATTCGG+GGG + chr3_3:56955203-56955222 None:intergenic 55.0%
TTCTGATGCACCTGCCACCA+AGG - chr3_3:56955562-56955581 Msa0459960:CDS 55.0%
GAAGAGGGTTTAGGAGGGGA+AGG - chr3_3:56956088-56956107 Msa0459960:intron 55.0%
AGGAGGGTTTAGGAGGGGAA+GGG + chr3_3:56956286-56956305 None:intergenic 55.0%
TTGGGAGGAGGGTTTAGGAG+GGG + chr3_3:56956291-56956310 None:intergenic 55.0%
GTTTGGGAGGAGGGTTTAGG+AGG + chr3_3:56956293-56956312 None:intergenic 55.0%
! CTCTTCCCCTGTGGGTGTTT+TGG - chr3_3:56957729-56957748 Msa0459960:intron 55.0%
!! TAGGGTGCCACCCATTTTGC+AGG + chr3_3:56957861-56957880 None:intergenic 55.0%
CTTGCCCTCTGGACAGAAAG+AGG - chr3_3:56957914-56957933 Msa0459960:CDS 55.0%
AGCTAGCACGATGCTGGGCA+TGG - chr3_3:56955349-56955368 Msa0459960:three_prime_UTR 60.0%
GCTAGCACGATGCTGGGCAT+GGG - chr3_3:56955350-56955369 Msa0459960:three_prime_UTR 60.0%
CTAGCACGATGCTGGGCATG+GGG - chr3_3:56955351-56955370 Msa0459960:three_prime_UTR 60.0%
AGGGTTTAGGAGGGGAAGGG+AGG - chr3_3:56956092-56956111 Msa0459960:intron 60.0%
GGGTTTAGGAGGGGAAGGGA+GGG - chr3_3:56956093-56956112 Msa0459960:intron 60.0%
GGTTTAGGAGGGGAAGGGAG+GGG - chr3_3:56956094-56956113 Msa0459960:intron 60.0%
GGTTTAGGAGGGGAAGGGAG+GGG + chr3_3:56956094-56956113 None:intergenic 60.0%
GGGTTTAGGAGGGGAAGGGA+GGG + chr3_3:56956093-56956112 None:intergenic 60.0%
AGGGTTTAGGAGGGGAAGGG+AGG + chr3_3:56956092-56956111 None:intergenic 60.0%
GAGGAGGGTTTAGGAGGGGA+AGG + chr3_3:56956287-56956306 None:intergenic 60.0%
TTAGGAGGGGAAGGGAGGGG+AGG - chr3_3:56956097-56956116 Msa0459960:intron 65.0%
TAGGAGGGGAAGGGAGGGGA+GGG + chr3_3:56956098-56956117 None:intergenic 65.0%
TTAGGAGGGGAAGGGAGGGG+AGG + chr3_3:56956097-56956116 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
chr3_3 gene 56955135 56958357 56955135 ID=Msa0459960;Name=Msa0459960
chr3_3 mRNA 56955135 56958357 56955135 ID=Msa0459960-mRNA-1;Parent=Msa0459960;Name=Msa0459960-mRNA-1;_AED=0.02;_eAED=0.02;_QI=224|1|1|1|1|1|3|410|216
chr3_3 exon 56955135 56955737 56955135 ID=Msa0459960-mRNA-1:exon:11488;Parent=Msa0459960-mRNA-1
chr3_3 exon 56956336 56956568 56956336 ID=Msa0459960-mRNA-1:exon:11487;Parent=Msa0459960-mRNA-1
chr3_3 exon 56957909 56958357 56957909 ID=Msa0459960-mRNA-1:exon:11486;Parent=Msa0459960-mRNA-1
chr3_3 five_prime_UTR 56958134 56958357 56958134 ID=Msa0459960-mRNA-1:five_prime_utr;Parent=Msa0459960-mRNA-1
chr3_3 CDS 56957909 56958133 56957909 ID=Msa0459960-mRNA-1:cds;Parent=Msa0459960-mRNA-1
chr3_3 CDS 56956336 56956568 56956336 ID=Msa0459960-mRNA-1:cds;Parent=Msa0459960-mRNA-1
chr3_3 CDS 56955545 56955737 56955545 ID=Msa0459960-mRNA-1:cds;Parent=Msa0459960-mRNA-1
chr3_3 three_prime_UTR 56955135 56955544 56955135 ID=Msa0459960-mRNA-1:three_prime_utr;Parent=Msa0459960-mRNA-1
Gene Sequence

>Msa0459960

ATGCTGGGCATGGGGTCCTTAACTTTGAGTTCACCAACTACAATGGTAGCTCAAACTACTCTTACCTCTTCCAAAAGGCACATCCTTTCTAACAGAACTCACTCTTTACCAATGAGTTTGGTCTCTCCTACAAACCAAAATTTCAAGAAACAAAAACCTTTTATCACTGCTGTTGCTTCTGTTCAATCTGACAACGTTAGTTCTTCTGATGCACCTGCCACCAAGAGTGAGTTCACCAAGTATTATTTTATTGTTGCAAATGCAAAATTTATGCTGGATGAGGAGGAGCATTTCCAAGAACAACTATTTGAACGCCGTCGCTACTATGGAGAACGTAATAAAGAACAGGATTACTGGCTTGTCATTGAGCCTAAGTTCTTGGATAGTTTTCCTGACTTTATCAAGAAACTGCGGAGACCTGCTGTTGCTCTAGTGTCAACCAACGGTCCTTGGATCACATTCATGAAATTAAGACTGGATCGAGTTTTATCAGGAAGTTTTGAAGCTGACAGCTTAGAAGAAGCATTAGCCTCCAATCCTACTAAGCTGGAATTTGAGAAGCCTGCAAAATGGGTGGCACCCTATCCTAAATATGAATCTGGGTGGTGGGAAACTTTCTTGCCCTCTGGACAGAAAGAGGTGAACTCTTGA
Protein sequence

>Msa0459960

MLGMGSLTLSSPTTMVAQTTLTSSKRHILSNRTHSLPMSLVSPTNQNFKKQKPFITAVASVQSDNVSSSDAPATKSEFTKYYFIVANAKFMLDEEEHFQEQLFERRRYYGERNKEQDYWLVIEPKFLDSFPDFIKKLRRPAVALVSTNGPWITFMKLRLDRVLSGSFEADSLEEALASNPTKLEFEKPAKWVAPYPKYESGWWETFLPSGQKEVNS*