AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1404370

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1404370 MtrunA17_Chr8g0335951 91.698 265 18 1 277 541 190 450 1.76e-174 500
Msa1404370 MtrunA17_Chr8g0335951 60.819 342 117 4 1 342 1 325 1.01e-131 391
Msa1404370 MtrunA17_Chr8g0349421 90.574 244 19 1 300 543 1 240 5.45e-159 453
Msa1404370 MtrunA17_Chr8g0349431 98.400 125 2 0 87 211 1 125 3.41e-84 257
Msa1404370 MtrunA17_Chr1g0185601 34.186 430 273 6 79 503 36 460 1.21e-80 261
Msa1404370 MtrunA17_Chr8g0368151 33.493 418 274 2 80 495 24 439 9.86e-80 258
Msa1404370 MtrunA17_Chr1g0194521 34.038 473 296 7 48 519 68 525 1.73e-78 256
Msa1404370 MtrunA17_Chr8g0359961 32.243 428 287 3 81 507 35 460 9.85e-74 243
Msa1404370 MtrunA17_Chr2g0324251 32.957 443 285 6 74 508 27 465 1.75e-72 239
Msa1404370 MtrunA17_Chr2g0303221 32.877 438 288 6 79 514 31 464 4.45e-71 236
Msa1404370 MtrunA17_Chr4g0021331 32.569 436 282 7 79 508 27 456 1.23e-69 232
Msa1404370 MtrunA17_Chr3g0132571 27.647 510 337 8 26 526 3 489 1.60e-68 229
Msa1404370 MtrunA17_Chr2g0282091 31.368 424 284 3 72 494 26 443 6.66e-66 222
Msa1404370 MtrunA17_Chr1g0155351 28.239 301 214 2 91 390 66 365 3.63e-41 153
Msa1404370 MtrunA17_Chr2g0289531 58.730 126 23 1 351 476 1 97 1.14e-40 142
Msa1404370 MtrunA17_Chr1g0171501 25.432 405 292 7 93 497 153 547 2.36e-29 122
Msa1404370 MtrunA17_Chr7g0256181 25.180 417 300 8 81 497 160 564 6.88e-29 121
Msa1404370 MtrunA17_Chr2g0328121 22.300 426 316 7 79 497 44 461 4.10e-28 117
Msa1404370 MtrunA17_Chr1g0168811 21.543 311 232 3 143 446 211 516 8.98e-19 90.1
Msa1404370 MtrunA17_Chr1g0168811 21.751 377 247 10 125 496 159 492 5.12e-11 65.5
Msa1404370 MtrunA17_Chr3g0143411 20.263 380 288 7 123 497 155 524 1.12e-16 83.2
Msa1404370 MtrunA17_Chr3g0099661 22.324 327 245 6 113 433 170 493 2.81e-16 82.4
Msa1404370 MtrunA17_Chr3g0099661 23.282 262 166 9 177 418 582 828 3.93e-12 69.3
Msa1404370 MtrunA17_Chr3g0099661 23.385 325 223 9 178 491 344 653 4.13e-11 65.9
Msa1404370 MtrunA17_Chr4g0059671 26.172 256 179 5 183 433 175 425 2.83e-16 82.4
Msa1404370 MtrunA17_Chr8g0359551 28.502 207 147 1 242 448 203 408 3.50e-16 82.0
Msa1404370 MtrunA17_Chr8g0359551 21.852 270 210 1 183 452 214 482 5.21e-13 71.6
Msa1404370 MtrunA17_Chr1g0200211 23.754 341 246 7 183 515 175 509 3.63e-16 82.0
Msa1404370 MtrunA17_Chr7g0220501 22.609 345 240 7 152 493 367 687 2.56e-15 79.3
Msa1404370 MtrunA17_Chr7g0220501 25.896 251 146 5 217 430 320 567 1.26e-11 67.8
Msa1404370 MtrunA17_Chr8g0377751 20.669 329 224 6 159 451 674 1001 3.86e-15 79.0
Msa1404370 MtrunA17_Chr5g0446031 19.943 351 274 4 103 451 406 751 5.05e-15 78.6
Msa1404370 MtrunA17_Chr5g0446031 22.984 248 181 3 199 446 579 816 3.25e-13 72.8
Msa1404370 MtrunA17_Chr5g0446031 22.340 376 269 11 183 549 205 566 3.64e-12 69.3
Msa1404370 MtrunA17_Chr2g0290441 20.217 277 217 3 180 453 218 493 3.31e-14 75.5
Msa1404370 MtrunA17_Chr2g0290441 22.222 279 215 2 174 451 106 383 4.32e-11 65.9
Msa1404370 MtrunA17_Chr3g0126831 22.420 281 210 3 238 518 287 559 4.00e-14 75.5
Msa1404370 MtrunA17_Chr4g0033981 22.727 264 200 2 183 446 324 583 4.56e-14 75.1
Msa1404370 MtrunA17_Chr7g0241721 20.885 407 313 5 128 531 3 403 4.93e-14 75.1
Msa1404370 MtrunA17_Chr2g0297991 19.467 375 292 7 67 433 117 489 7.74e-14 74.7
Msa1404370 MtrunA17_Chr2g0297991 22.823 333 243 8 180 507 377 700 1.35e-11 67.4
Msa1404370 MtrunA17_Chr2g0297991 22.667 300 214 6 158 452 537 823 2.50e-11 66.6
Msa1404370 MtrunA17_Chr4g0037841 20.795 327 246 6 183 503 237 556 1.56e-13 73.2
Msa1404370 MtrunA17_Chr4g0060341 20.795 327 246 6 183 503 237 556 1.69e-13 73.2
Msa1404370 MtrunA17_Chr3g0136851 23.843 281 203 6 152 426 274 549 5.99e-13 72.0
Msa1404370 MtrunA17_Chr8g0368891 19.926 271 216 1 183 453 383 652 8.30e-13 71.2
Msa1404370 MtrunA17_Chr5g0396041 20.501 439 330 9 109 537 193 622 8.42e-13 71.6
Msa1404370 MtrunA17_Chr8g0392911 22.642 265 200 3 184 446 198 459 1.19e-12 70.9
Msa1404370 MtrunA17_Chr8g0353181 23.615 343 218 7 183 525 202 500 1.31e-12 70.5
Msa1404370 MtrunA17_Chr4g0054821 24.522 314 210 7 175 479 254 549 2.15e-12 70.1
Msa1404370 MtrunA17_Chr8g0382891 22.193 374 230 10 104 426 73 436 2.99e-12 68.9
Msa1404370 MtrunA17_Chr7g0220481 23.214 280 203 4 153 430 263 532 3.83e-12 69.3
Msa1404370 MtrunA17_Chr8g0369641 19.935 306 236 4 180 483 490 788 4.48e-12 68.9
Msa1404370 MtrunA17_Chr2g0331911 23.077 312 232 5 199 507 178 484 7.04e-12 68.2
Msa1404370 MtrunA17_Chr1g0162281 20.060 334 257 5 178 508 226 552 8.45e-12 68.2
Msa1404370 MtrunA17_Chr8g0387281 21.587 315 233 5 128 435 332 639 8.51e-12 67.8
Msa1404370 MtrunA17_Chr8g0387281 23.410 346 250 7 183 518 239 579 9.45e-12 67.8
Msa1404370 MtrunA17_Chr7g0214351 21.835 316 235 7 183 494 313 620 8.87e-12 67.8
Msa1404370 MtrunA17_Chr5g0394721 21.407 327 243 6 114 434 206 524 9.54e-12 67.8
Msa1404370 MtrunA17_Chr5g0394721 22.807 285 214 3 179 459 236 518 6.68e-11 65.1
Msa1404370 MtrunA17_Chr7g0246931 21.409 369 272 10 93 452 157 516 9.57e-12 67.4
Msa1404370 MtrunA17_Chr7g0256621 22.013 318 231 6 184 494 153 460 1.36e-11 67.0
Msa1404370 MtrunA17_Chr1g0210801 18.896 471 329 11 108 535 97 557 1.58e-11 67.0
Msa1404370 MtrunA17_Chr6g0478691 21.053 323 249 3 183 504 245 562 1.70e-11 67.0
Msa1404370 MtrunA17_Chr2g0285331 20.900 311 241 2 198 507 284 590 1.87e-11 67.0
Msa1404370 MtrunA17_Chr8g0391651 22.182 275 209 3 180 452 416 687 2.01e-11 67.0
Msa1404370 MtrunA17_Chr2g0332881 22.067 358 259 8 172 524 266 608 2.44e-11 66.6
Msa1404370 MtrunA17_Chr6g0458411 20.173 347 265 5 178 523 231 566 2.63e-11 66.2
Msa1404370 MtrunA17_Chr6g0458411 22.857 385 244 9 184 523 98 474 4.67e-11 65.5
Msa1404370 MtrunA17_Chr7g0215561 21.656 314 238 5 183 494 312 619 3.49e-11 65.9
Msa1404370 MtrunA17_Chr8g0365781 22.376 362 247 6 96 433 494 845 3.67e-11 66.2
Msa1404370 MtrunA17_Chr1g0170071 26.380 163 117 2 170 330 675 836 4.55e-11 65.9
Msa1404370 MtrunA17_Chr4g0038401 24.380 242 168 6 212 446 354 587 5.55e-11 65.5
Msa1404370 MtrunA17_Chr4g0060641 20.760 342 255 5 172 506 411 743 5.73e-11 65.5
Msa1404370 MtrunA17_Chr7g0241871 21.270 315 239 4 186 498 99 406 5.90e-11 65.5
Msa1404370 MtrunA17_Chr2g0296401 24.901 253 176 6 181 422 239 488 8.24e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1404370 AT1G02150.1 58.527 516 203 3 10 523 3 509 0.0 633
Msa1404370 AT1G60770.1 32.289 415 277 2 81 493 25 437 9.75e-77 251
Msa1404370 AT4G02820.1 32.029 409 273 4 99 507 81 484 2.00e-74 246
Msa1404370 AT4G01990.1 33.796 432 282 3 77 507 37 465 1.24e-73 243
Msa1404370 AT2G20710.1 33.254 421 277 4 78 497 37 454 1.21e-72 240
Msa1404370 AT1G02370.1 34.524 420 260 6 81 492 73 485 1.78e-72 241
Msa1404370 AT5G27460.1 31.121 437 296 2 83 515 42 477 2.70e-68 229
Msa1404370 AT4G21705.1 30.046 436 303 2 76 510 22 456 7.19e-67 225
Msa1404370 AT2G20710.2 34.561 353 228 3 146 497 9 359 2.39e-62 210
Msa1404370 AT5G09450.1 31.746 315 207 4 71 383 52 360 7.10e-50 177
Msa1404370 AT1G28020.1 31.233 365 235 5 49 411 19 369 3.63e-45 166
Msa1404370 AT1G80270.1 27.273 385 270 6 77 460 128 503 2.01e-36 143
Msa1404370 AT1G80270.3 27.273 385 270 6 77 460 128 503 2.01e-36 143
Msa1404370 AT1G80270.2 27.273 385 270 6 77 460 128 503 2.01e-36 143
Msa1404370 AT1G80270.5 27.273 385 270 6 77 460 128 503 2.01e-36 143
Msa1404370 AT1G80270.4 27.273 385 270 6 77 460 128 503 2.01e-36 143
Msa1404370 AT1G15480.1 25.806 434 298 10 81 507 130 546 4.08e-33 134
Msa1404370 AT1G07590.1 23.587 407 303 5 96 498 82 484 2.11e-27 116
Msa1404370 AT3G11380.1 30.945 307 161 10 117 411 81 348 1.65e-25 110
Msa1404370 AT3G11380.1 30.899 178 120 3 98 273 365 541 1.25e-14 77.0
Msa1404370 AT3G15590.1 26.173 405 280 9 98 497 160 550 2.99e-25 110
Msa1404370 AT3G15590.2 26.173 405 280 9 98 497 160 550 2.99e-25 110
Msa1404370 AT5G02860.1 24.848 330 239 6 110 433 134 460 8.72e-19 90.9
Msa1404370 AT5G02860.1 23.053 321 242 2 180 500 313 628 8.90e-16 81.3
Msa1404370 AT5G02860.4 24.848 330 239 6 110 433 134 460 8.72e-19 90.9
Msa1404370 AT5G02860.4 23.053 321 242 2 180 500 313 628 8.90e-16 81.3
Msa1404370 AT5G02860.2 24.848 330 239 6 110 433 134 460 8.72e-19 90.9
Msa1404370 AT5G02860.2 23.053 321 242 2 180 500 313 628 8.90e-16 81.3
Msa1404370 AT5G02860.5 24.848 330 239 6 110 433 134 460 8.72e-19 90.9
Msa1404370 AT5G02860.5 23.053 321 242 2 180 500 313 628 8.90e-16 81.3
Msa1404370 AT5G02860.3 24.848 330 239 6 110 433 134 460 8.72e-19 90.9
Msa1404370 AT5G02860.3 23.053 321 242 2 180 500 313 628 8.90e-16 81.3
Msa1404370 AT1G28000.1 26.800 250 178 5 99 344 53 301 9.35e-19 89.0
Msa1404370 AT1G03560.1 23.792 269 203 2 180 447 256 523 1.64e-15 80.1
Msa1404370 AT4G30825.1 23.077 338 229 10 130 452 542 863 2.85e-15 79.7
Msa1404370 AT1G74580.2 22.222 333 236 8 174 495 413 733 3.01e-15 79.3
Msa1404370 AT1G74580.1 22.222 333 236 8 174 495 413 733 3.01e-15 79.3
Msa1404370 AT1G74580.3 22.222 333 236 8 174 495 413 733 3.01e-15 79.3
Msa1404370 AT5G01110.1 21.379 290 211 6 222 508 425 700 3.21e-15 79.3
Msa1404370 AT5G01110.1 21.289 357 266 6 102 452 158 505 1.37e-11 67.8
Msa1404370 AT3G18110.1 22.529 435 320 10 108 535 153 577 4.07e-15 79.3
Msa1404370 AT5G01110.2 21.379 290 211 6 222 508 453 728 4.32e-15 79.0
Msa1404370 AT5G01110.2 21.289 357 266 6 102 452 186 533 1.63e-11 67.8
Msa1404370 AT1G30290.2 21.408 341 258 4 188 522 284 620 1.71e-14 77.0
Msa1404370 AT1G30290.1 21.408 341 258 4 188 522 284 620 2.06e-14 77.0
Msa1404370 AT2G18940.1 23.220 323 239 5 180 500 350 665 3.09e-14 76.3
Msa1404370 AT3G06920.2 19.828 464 321 10 97 521 203 654 3.75e-14 76.3
Msa1404370 AT3G06920.3 19.828 464 321 10 97 521 203 654 3.75e-14 76.3
Msa1404370 AT3G06920.1 19.785 465 322 10 97 522 189 641 4.66e-14 75.9
Msa1404370 AT2G31400.2 22.360 322 209 9 183 493 411 702 5.63e-14 75.5
Msa1404370 AT2G31400.2 20.782 409 315 5 117 521 202 605 2.12e-12 70.5
Msa1404370 AT2G31400.1 22.360 322 209 9 183 493 411 702 6.65e-14 75.5
Msa1404370 AT2G31400.1 20.782 409 315 5 117 521 202 605 2.24e-12 70.5
Msa1404370 AT1G63150.2 24.354 271 198 5 180 447 152 418 2.27e-13 73.2
Msa1404370 AT1G63150.1 24.354 271 198 5 180 447 152 418 2.27e-13 73.2
Msa1404370 AT2G32630.1 19.294 425 298 7 122 508 200 617 4.49e-13 72.4
Msa1404370 AT5G65560.1 23.396 265 199 4 183 446 395 656 5.05e-13 72.4
Msa1404370 AT5G65560.2 23.396 265 199 4 183 446 395 656 5.05e-13 72.4
Msa1404370 AT5G65560.3 23.396 265 199 4 183 446 395 656 5.05e-13 72.4
Msa1404370 AT5G46580.1 22.615 283 214 4 175 453 250 531 6.35e-13 72.0
Msa1404370 AT1G43010.1 27.706 231 146 6 84 307 41 257 8.33e-13 68.9
Msa1404370 AT1G63230.1 21.118 322 240 6 180 498 9 319 2.05e-12 68.9
Msa1404370 AT3G22670.1 20.087 229 176 4 105 330 197 421 2.31e-12 70.1
Msa1404370 AT1G51965.1 21.322 469 295 15 109 517 113 567 2.35e-12 70.1
Msa1404370 AT5G25630.1 27.072 181 125 4 180 358 259 434 3.17e-12 69.7
Msa1404370 AT5G25630.3 27.072 181 125 4 180 358 208 383 3.30e-12 69.3
Msa1404370 AT4G11690.2 20.482 332 259 2 178 509 203 529 3.38e-12 69.3
Msa1404370 AT4G11690.1 20.482 332 259 2 178 509 230 556 3.41e-12 69.3
Msa1404370 AT1G20300.1 19.938 321 235 5 123 433 163 471 3.80e-12 69.3
Msa1404370 AT1G62590.2 24.277 346 241 7 117 452 231 565 3.95e-12 69.3
Msa1404370 AT1G62590.1 24.277 346 241 7 117 452 231 565 3.95e-12 69.3
Msa1404370 AT1G74900.1 22.096 353 256 9 110 452 52 395 4.01e-12 68.9
Msa1404370 AT2G35030.1 19.849 398 272 12 116 501 4 366 5.94e-12 68.9
Msa1404370 AT2G15630.1 20.728 357 247 5 183 507 262 614 6.15e-12 68.9
Msa1404370 AT1G63330.3 24.233 326 226 8 139 452 174 490 7.95e-12 68.2
Msa1404370 AT1G63330.1 24.233 326 226 8 139 452 174 490 7.95e-12 68.2
Msa1404370 AT1G63330.2 24.233 326 226 8 139 452 174 490 7.95e-12 68.2
Msa1404370 AT4G19890.1 23.750 320 224 7 183 494 324 631 8.31e-12 68.6
Msa1404370 AT1G10910.2 19.597 347 238 5 131 440 118 460 1.15e-11 67.8
Msa1404370 AT1G10910.1 19.597 347 238 5 131 440 118 460 1.18e-11 67.8
Msa1404370 AT5G04810.1 26.344 186 134 2 159 342 670 854 1.69e-11 67.8
Msa1404370 AT1G62910.1 21.324 408 304 7 117 518 229 625 1.73e-11 67.4
Msa1404370 AT1G62670.2 22.727 352 224 10 183 495 153 495 2.46e-11 67.0
Msa1404370 AT1G62670.1 22.727 352 224 10 183 495 153 495 2.46e-11 67.0
Msa1404370 AT4G31850.1 20.764 419 315 6 40 452 226 633 2.84e-11 67.0
Msa1404370 AT4G31850.1 21.184 321 240 6 180 496 292 603 3.34e-11 67.0
Msa1404370 AT5G16640.1 22.059 340 254 6 116 450 86 419 2.99e-11 66.2
Msa1404370 AT4G20090.2 21.569 357 266 7 127 476 164 513 3.32e-11 66.6
Msa1404370 AT4G20090.2 22.277 202 156 1 183 384 329 529 8.49e-11 65.1
Msa1404370 AT4G20090.1 21.569 357 266 7 127 476 164 513 3.32e-11 66.6
Msa1404370 AT4G20090.1 22.277 202 156 1 183 384 329 529 8.49e-11 65.1
Msa1404370 AT5G48730.1 22.455 334 243 7 150 474 149 475 3.44e-11 66.2
Msa1404370 AT3G09060.2 18.182 363 281 6 182 538 115 467 3.98e-11 66.2
Msa1404370 AT3G09060.1 18.182 363 281 6 182 538 115 467 3.98e-11 66.2
Msa1404370 AT5G42310.1 22.393 326 238 7 180 500 373 688 4.78e-11 66.2
Msa1404370 AT4G39620.2 22.289 332 241 10 117 437 103 428 5.54e-11 65.5
Msa1404370 AT5G39980.1 19.022 368 287 5 180 546 259 616 6.37e-11 65.5
Msa1404370 AT4G39620.1 22.289 332 241 10 117 437 103 428 8.21e-11 65.1
Msa1404370 AT5G61990.1 20.385 260 201 3 178 433 554 811 8.56e-11 65.5
Msa1404370 AT1G63070.1 18.873 355 274 4 172 518 235 583 9.29e-11 65.1

Find 111 sgRNAs with CRISPR-Local

Find 271 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGACTTGTGTCTTTAAATT+TGG 0.114196 tig0023062:+32612 Msa1404370:CDS
TATAGCATTCCATTTCAAAT+TGG 0.213868 tig0023062:-29112 None:intergenic
AGCTCCCATGTAGTCGAATT+TGG 0.237732 tig0023062:-31839 None:intergenic
ATCCTACCATTATTCCTAAT+TGG 0.237984 tig0023062:+31405 Msa1404370:CDS
CGACTTTATCATAATCCTTA+AGG 0.296327 tig0023062:-31250 None:intergenic
TCGAAGTAATGAGGAGTAAA+AGG 0.304308 tig0023062:+31177 Msa1404370:CDS
TTCTGAGGCGCTTTCGTGCT+TGG 0.317772 tig0023062:+31895 Msa1404370:CDS
GTACTAAATGTACTCCAATT+AGG 0.322076 tig0023062:-31419 None:intergenic
AAAGGGAAAGAGAAACGTTT+TGG 0.332422 tig0023062:-28939 None:intergenic
GAAACCCAAGCCAATAAAAT+TGG 0.333547 tig0023062:+31952 Msa1404370:CDS
AAAACATGATAGAGCTCTTC+AGG 0.338362 tig0023062:+29266 Msa1404370:CDS
TGTACTCCAATTAGGAATAA+TGG 0.340858 tig0023062:-31411 None:intergenic
GATGATGGAGAATCCTGAAT+TGG 0.341809 tig0023062:+29149 Msa1404370:CDS
TTTCGTCTTGACAAAGCTTA+AGG 0.346882 tig0023062:-31982 None:intergenic
CATTATCTCCTGAGTTTGTA+TGG 0.359485 tig0023062:+31542 Msa1404370:CDS
CTGCTTCTGTGTTGAATCAA+TGG 0.369186 tig0023062:+29175 Msa1404370:CDS
AAGGCCGAGGGTAGAATATT+AGG 0.374501 tig0023062:+31500 Msa1404370:CDS
ACTGACAGTGGAATTGGAAT+TGG 0.384505 tig0023062:+29012 Msa1404370:CDS
ATAGCATTCCATTTCAAATT+GGG 0.388781 tig0023062:-29111 None:intergenic
ATTGAGTTGTTGAAGAAGTC+GGG 0.392725 tig0023062:+32031 Msa1404370:CDS
TATTTCTTCTCTCGTAAGAA+TGG 0.397940 tig0023062:+31655 Msa1404370:CDS
GGGTCATATTGCTGAAAATA+GGG 0.397978 tig0023062:+31871 Msa1404370:CDS
ATATATATACATATAATATC+TGG 0.400262 tig0023062:+31315 Msa1404370:CDS
AGGGTCATATTGCTGAAAAT+AGG 0.400702 tig0023062:+31870 Msa1404370:CDS
CCCTCTGATGATTCTAGAAT+AGG 0.408766 tig0023062:+31728 Msa1404370:CDS
AGAGAAAACCCAATTTGAAA+TGG 0.410586 tig0023062:+29103 Msa1404370:CDS
CCGCATACGCTTCGTCGTTA+TGG 0.412804 tig0023062:-32057 None:intergenic
AACTCTGCAAAGTTCCCATT+TGG 0.415800 tig0023062:-29220 None:intergenic
CAGAAAAGCTTTACGAGGAA+TGG 0.415843 tig0023062:+31693 Msa1404370:CDS
GTTTCCAAGTATACCTAACT+TGG 0.418407 tig0023062:+31619 Msa1404370:CDS
AAGGAAGGAATCTCACCAAA+TGG 0.426265 tig0023062:+29205 Msa1404370:CDS
CACATTGACCCTTTGTTTGA+AGG 0.426662 tig0023062:-28983 None:intergenic
ACAGCATGGTATCCCAAGTT+AGG 0.429036 tig0023062:-31632 None:intergenic
AAGAAGATTGAATTTGAAGA+GGG 0.436849 tig0023062:-28909 None:intergenic
TTAAAAGACAAGAGAACATA+TGG 0.437458 tig0023062:+31098 Msa1404370:CDS
AATTGAGTTGTTGAAGAAGT+CGG 0.440073 tig0023062:+32030 Msa1404370:CDS
ATTCTTTCAATCCTGTTTGA+CGG 0.440996 tig0023062:-32100 None:intergenic
GAAGAGTATGAGAATAAGAA+AGG 0.442055 tig0023062:-28957 None:intergenic
ACGAGGAATGGGTTTCTGTA+AGG 0.443573 tig0023062:+31705 Msa1404370:CDS
TGCTTCTGTGTTGAATCAAT+GGG 0.447177 tig0023062:+29176 Msa1404370:CDS
GCAAAGATGAACCGTCAAAC+AGG 0.449173 tig0023062:+32089 Msa1404370:CDS
CGACAAGTGATTCAAAGAGT+TGG 0.450702 tig0023062:+31930 Msa1404370:CDS
ATCTCAAAGATTCACAGTTA+TGG 0.451129 tig0023062:+29066 Msa1404370:CDS
AGAGTCATCATTAAATTAAA+CGG 0.454778 tig0023062:-31218 None:intergenic
CGTAAGAATGGACGATATTG+AGG 0.465330 tig0023062:+31667 Msa1404370:CDS
ATACAAGAGGATTTCGATGA+TGG 0.469256 tig0023062:+29134 Msa1404370:CDS
AGAAAAGCTTTACGAGGAAT+GGG 0.471834 tig0023062:+31694 Msa1404370:CDS
TCAAGGATCAATAGAGAAGA+TGG 0.473452 tig0023062:+31355 Msa1404370:CDS
ACAGAAGCAGAACCCAATTC+AGG 0.475894 tig0023062:-29162 None:intergenic
TATCCCAAGTTAGGTATACT+TGG 0.477102 tig0023062:-31623 None:intergenic
GGCTGCAATGTATATTAAGA+TGG 0.477129 tig0023062:+31445 Msa1404370:CDS
GATCTAATCGCAAGAGTTCA+CGG 0.482500 tig0023062:+31032 Msa1404370:CDS
GACTCTGTACATGAACCTTA+AGG 0.486004 tig0023062:+31235 Msa1404370:CDS
ATGATGGAGAATCCTGAATT+GGG 0.490611 tig0023062:+29150 Msa1404370:CDS
AATATCTGGTTGTCCTCTTG+TGG 0.497939 tig0023062:+31329 Msa1404370:CDS
ATAAGCTGGTATCTTAAGAA+AGG 0.498356 tig0023062:+31761 Msa1404370:CDS
TTATGATAAAGTCGATATGT+TGG 0.499930 tig0023062:+31259 Msa1404370:CDS
GCAGATATTCTAAACCTCTC+TGG 0.506381 tig0023062:-30987 None:intergenic
TTGTCCTCTTGTGGATCTCA+AGG 0.509360 tig0023062:+31338 Msa1404370:CDS
ACATTGACCCTTTGTTTGAA+GGG 0.509737 tig0023062:-28982 None:intergenic
TAGGAAACCTTCTCATAAGC+TGG 0.515507 tig0023062:+31747 Msa1404370:CDS
CACGGAGTTTCTAGTGCAGA+AGG 0.519926 tig0023062:+31050 Msa1404370:CDS
CTTCTGATGCATGTGATATG+AGG 0.519967 tig0023062:+32568 Msa1404370:CDS
AAGAGTATGAGAATAAGAAA+GGG 0.530885 tig0023062:-28956 None:intergenic
GACAGTGGAATTGGAATTGG+AGG 0.533917 tig0023062:+29015 Msa1404370:CDS
TACATGGGAGCTTCTTTCTG+AGG 0.536998 tig0023062:+31850 Msa1404370:CDS
GATGTCCAAATTCGACTACA+TGG 0.539568 tig0023062:+31834 Msa1404370:CDS
CAACACTTCCATACAAACTC+AGG 0.539796 tig0023062:-31550 None:intergenic
CTGAGTTTGTATGGAAGTGT+TGG 0.541520 tig0023062:+31551 Msa1404370:CDS
TTATCTTCAACAAACAACAT+TGG 0.542696 tig0023062:+28845 None:intergenic
AGGAAGGAATCTCACCAAAT+GGG 0.547368 tig0023062:+29206 Msa1404370:CDS
TTCTTTAAGCATATGAGTGA+AGG 0.550405 tig0023062:+31806 Msa1404370:CDS
TGCTGAAAATAGGGTTTCTG+AGG 0.551041 tig0023062:+31880 Msa1404370:CDS
CATTGACCCTTTGTTTGAAG+GGG 0.551949 tig0023062:-28981 None:intergenic
AGGACGAAGTTTACCGCGTG+TGG 0.552160 tig0023062:+31579 Msa1404370:CDS
GTAAGAATGGACGATATTGA+GGG 0.554710 tig0023062:+31668 Msa1404370:CDS
GTATGGAAGTGTTGGAAACA+AGG 0.554809 tig0023062:+31559 Msa1404370:CDS
ATCAATGGGAGAATGAAGGA+AGG 0.564566 tig0023062:+29190 Msa1404370:CDS
AAACTCAGGAGATAATGAAA+AGG 0.565979 tig0023062:-31536 None:intergenic
TCGACCTAATATTCTACCCT+CGG 0.566861 tig0023062:-31504 None:intergenic
AAAGAAGATTGAATTTGAAG+AGG 0.567795 tig0023062:-28910 None:intergenic
AATCTTTACTCGAAGTAATG+AGG 0.567840 tig0023062:+31168 Msa1404370:CDS
TCATTCTAGATCAAGAGAAA+AGG 0.571003 tig0023062:+31142 Msa1404370:CDS
CAACAACTCAATTAAAACCT+CGG 0.574815 tig0023062:-32020 None:intergenic
TAAGATACCAGCTTATGAGA+AGG 0.579937 tig0023062:-31754 None:intergenic
TTGGAGTACATTTAGTACAA+TGG 0.583230 tig0023062:+31424 Msa1404370:CDS
TTTCCAAGTATACCTAACTT+GGG 0.589423 tig0023062:+31620 Msa1404370:CDS
TTTAAGCATATGAGTGAAGG+TGG 0.593303 tig0023062:+31809 Msa1404370:CDS
ACATGGGAGCTTCTTTCTGA+GGG 0.601564 tig0023062:+31851 Msa1404370:CDS
AATGAACATATGCATTCAAG+AGG 0.604245 tig0023062:-31124 None:intergenic
TCCTATTCTAGAATCATCAG+AGG 0.618071 tig0023062:-31729 None:intergenic
ATAGAAAACATACACAAGGA+TGG 0.618846 tig0023062:-32591 None:intergenic
ATTGACCCTTTGTTTGAAGG+GGG 0.620972 tig0023062:-28980 None:intergenic
TTGAATCAATGGGAGAATGA+AGG 0.623335 tig0023062:+29186 Msa1404370:CDS
AATGGAATGCTATATACAAG+AGG 0.631419 tig0023062:+29121 Msa1404370:CDS
AGAAGATTGAATTTGAAGAG+GGG 0.639018 tig0023062:-28908 None:intergenic
TTGACCCTTTGTTTGAAGGG+GGG 0.640200 tig0023062:-28979 None:intergenic
TTGTCAAGACGAAAACGACA+TGG 0.642627 tig0023062:+31991 Msa1404370:CDS
CCTATTCTAGAATCATCAGA+GGG 0.647606 tig0023062:-31728 None:intergenic
ATGAACATATGCATTCAAGA+GGG 0.654487 tig0023062:-31123 None:intergenic
AAGCATATGAGTGAAGGTGG+TGG 0.657423 tig0023062:+31812 Msa1404370:CDS
ATGTCCAAATTCGACTACAT+GGG 0.669288 tig0023062:+31835 Msa1404370:CDS
TGATCCTTGAGATCCACAAG+AGG 0.674298 tig0023062:-31342 None:intergenic
CGAGAGAAGAAATAACAGCA+TGG 0.674858 tig0023062:-31646 None:intergenic
CTCCAATTAGGAATAATGGT+AGG 0.679561 tig0023062:-31407 None:intergenic
TAAGAATGGACGATATTGAG+GGG 0.688947 tig0023062:+31669 Msa1404370:CDS
AAGAATGGACGATATTGAGG+GGG 0.691386 tig0023062:+31670 Msa1404370:CDS
ATTTATAGTTATTCCACACG+CGG 0.697764 tig0023062:-31592 None:intergenic
AGTCATAGAAAACATACACA+AGG 0.711577 tig0023062:-32595 None:intergenic
AAACGACATGGAGAGCGCCG+AGG 0.711901 tig0023062:+32003 Msa1404370:CDS
GGATGAACAATAGACCAGAG+AGG 0.722678 tig0023062:+30973 Msa1404370:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATATACATATAATATC+TGG + tig0023062:31315-31334 Msa1404370:CDS 10.0%
!! TCATTATATGTTATTCTATA+TGG + tig0023062:29305-29324 Msa1404370:intron 15.0%
!! AAACAAAAAATATGATTGAA+AGG - tig0023062:30272-30291 None:intergenic 15.0%
!! AAAAAATTTATATCAGTTTG+AGG - tig0023062:30526-30545 None:intergenic 15.0%
!!! AAGAATTGTGTTTTTTAATC+AGG - tig0023062:29868-29887 None:intergenic 20.0%
!!! TAGAATTCTTGTTTATTTTC+CGG - tig0023062:29923-29942 None:intergenic 20.0%
!! ACACACACAAAAAAAAAAAA+AGG + tig0023062:30088-30107 Msa1404370:intron 20.0%
!!! GTTAAAGGTTTTTGATTTTT+TGG - tig0023062:30327-30346 None:intergenic 20.0%
!! GTAGTAAAAGAAATACATAA+CGG + tig0023062:30493-30512 Msa1404370:intron 20.0%
!!! GGAAATTTTCAATATATTCA+GGG - tig0023062:30665-30684 None:intergenic 20.0%
!!! AGGAAATTTTCAATATATTC+AGG - tig0023062:30666-30685 None:intergenic 20.0%
!! TGATATTAAGACATTATTTG+AGG - tig0023062:30895-30914 None:intergenic 20.0%
!! AGAGTCATCATTAAATTAAA+CGG - tig0023062:31221-31240 None:intergenic 20.0%
! AAGAAGATTGAATTTGAAGA+GGG - tig0023062:28912-28931 None:intergenic 25.0%
! AAAGAAGATTGAATTTGAAG+AGG - tig0023062:28913-28932 None:intergenic 25.0%
!! AAGAGTATGAGAATAAGAAA+GGG - tig0023062:28959-28978 None:intergenic 25.0%
! ATAGCATTCCATTTCAAATT+GGG - tig0023062:29114-29133 None:intergenic 25.0%
! TATAGCATTCCATTTCAAAT+TGG - tig0023062:29115-29134 None:intergenic 25.0%
!!! TGTGTTTTTTAATCAGGAAA+TGG - tig0023062:29862-29881 None:intergenic 25.0%
!!! AGAATTCTTGTTTATTTTCC+GGG - tig0023062:29922-29941 None:intergenic 25.0%
! TTAAGTTTAAATGTCTCTGT+TGG - tig0023062:29991-30010 None:intergenic 25.0%
! AAATCATAATGTATCATGAG+AGG - tig0023062:30155-30174 None:intergenic 25.0%
! TTTGGGGATAGAAATTATTA+AGG - tig0023062:30244-30263 None:intergenic 25.0%
!! ATGATTGAAAGGTATGATTT+GGG - tig0023062:30261-30280 None:intergenic 25.0%
!! TATGATTGAAAGGTATGATT+TGG - tig0023062:30262-30281 None:intergenic 25.0%
! TTAAAACTTGTAGTGGTTAA+AGG - tig0023062:30342-30361 None:intergenic 25.0%
!! ACACAATTTAAAACTTGTAG+TGG - tig0023062:30349-30368 None:intergenic 25.0%
!! ACTGACCAATACAATTTTTA+GGG - tig0023062:30399-30418 None:intergenic 25.0%
!! ATTTCTTTTACTACGTTTCT+TGG - tig0023062:30487-30506 None:intergenic 25.0%
!! TAAAAGAAATACATAACGGA+CGG + tig0023062:30497-30516 Msa1404370:intron 25.0%
!! ACACCAACGTTAAAATTTTA+TGG + tig0023062:30575-30594 Msa1404370:intron 25.0%
!! CACCAACGTTAAAATTTTAT+GGG + tig0023062:30576-30595 Msa1404370:intron 25.0%
!! ACCAACGTTAAAATTTTATG+GGG + tig0023062:30577-30596 Msa1404370:intron 25.0%
!!! TTTTTTTTTACCAACAGTCT+AGG - tig0023062:30686-30705 None:intergenic 25.0%
! TACTTTCAAAAAATCATTCG+TGG - tig0023062:30919-30938 None:intergenic 25.0%
!!! TGTCTTTTAAATCATTGGTT+AGG - tig0023062:31088-31107 None:intergenic 25.0%
!! TCTCTTGTCTTTTAAATCAT+TGG - tig0023062:31093-31112 None:intergenic 25.0%
! TTAAAAGACAAGAGAACATA+TGG + tig0023062:31098-31117 Msa1404370:CDS 25.0%
! TTATGATAAAGTCGATATGT+TGG + tig0023062:31259-31278 Msa1404370:CDS 25.0%
! TTGAATGTAATAACATGTGT+TGG - tig0023062:32279-32298 None:intergenic 25.0%
!!! TCAAATTTTGTTTTGACTTG+TGG + tig0023062:32401-32420 Msa1404370:intron 25.0%
!!! AAAATGAGTTGATAGAACAT+AGG + tig0023062:32424-32443 Msa1404370:intron 25.0%
!!! AAATGAGTTGATAGAACATA+GGG + tig0023062:32425-32444 Msa1404370:intron 25.0%
!! TTCTTTTTCTCAAGCTTTTA+TGG + tig0023062:32463-32482 Msa1404370:intron 25.0%
!! ATGACTTGTGTCTTTAAATT+TGG + tig0023062:32612-32631 Msa1404370:CDS 25.0%
AGAAGATTGAATTTGAAGAG+GGG - tig0023062:28911-28930 None:intergenic 30.0%
! GAAGAGTATGAGAATAAGAA+AGG - tig0023062:28960-28979 None:intergenic 30.0%
ATCTCAAAGATTCACAGTTA+TGG + tig0023062:29066-29085 Msa1404370:CDS 30.0%
AGAGAAAACCCAATTTGAAA+TGG + tig0023062:29103-29122 Msa1404370:CDS 30.0%
AATGGAATGCTATATACAAG+AGG + tig0023062:29121-29140 Msa1404370:CDS 30.0%
TTGCTTCATAATGTTAAGGA+GGG - tig0023062:29455-29474 None:intergenic 30.0%
GTTCAATTCAATCTGTTGTT+CGG - tig0023062:29492-29511 None:intergenic 30.0%
TCCAAAAGAGTAAGAATGAA+CGG + tig0023062:29512-29531 Msa1404370:intron 30.0%
!! GAATTCTTGTTTATTTTCCG+GGG - tig0023062:29921-29940 None:intergenic 30.0%
CTAGAAGACATTAATGCAAT+AGG + tig0023062:29940-29959 Msa1404370:intron 30.0%
AATATATTCAGCTGCACAAT+TGG - tig0023062:30038-30057 None:intergenic 30.0%
AATTGTGATAGCTAAAGTGT+CGG - tig0023062:30214-30233 None:intergenic 30.0%
! TGATTGAAAGGTATGATTTG+GGG - tig0023062:30260-30279 None:intergenic 30.0%
ATCCACCCTAAAAATTGTAT+TGG + tig0023062:30391-30410 Msa1404370:intron 30.0%
! CACTGACCAATACAATTTTT+AGG - tig0023062:30400-30419 None:intergenic 30.0%
! CTAAAAATTGTATTGGTCAG+TGG + tig0023062:30398-30417 Msa1404370:intron 30.0%
TTGGATTTCATAGATGACTA+AGG - tig0023062:30468-30487 None:intergenic 30.0%
TTGAAAATTTCCTAGACTGT+TGG + tig0023062:30673-30692 Msa1404370:intron 30.0%
ACATGTGGATAGTATAAGAA+TGG - tig0023062:30730-30749 None:intergenic 30.0%
TTCCGTTTAGTAGAATCATA+GGG - tig0023062:30807-30826 None:intergenic 30.0%
ATTCCGTTTAGTAGAATCAT+AGG - tig0023062:30808-30827 None:intergenic 30.0%
! TTAAGACATTATTTGAGGCA+AGG - tig0023062:30890-30909 None:intergenic 30.0%
! ATGTTTTGCAGGTATATGAT+TGG + tig0023062:30952-30971 Msa1404370:intron 30.0%
ATGAACATATGCATTCAAGA+GGG - tig0023062:31126-31145 None:intergenic 30.0%
AATGAACATATGCATTCAAG+AGG - tig0023062:31127-31146 None:intergenic 30.0%
TCATTCTAGATCAAGAGAAA+AGG + tig0023062:31142-31161 Msa1404370:CDS 30.0%
AATCTTTACTCGAAGTAATG+AGG + tig0023062:31168-31187 Msa1404370:CDS 30.0%
CGACTTTATCATAATCCTTA+AGG - tig0023062:31253-31272 None:intergenic 30.0%
! AGTTTTTGAACAGATGTCAA+AGG + tig0023062:31382-31401 Msa1404370:CDS 30.0%
ATCCTACCATTATTCCTAAT+TGG + tig0023062:31405-31424 Msa1404370:CDS 30.0%
TGTACTCCAATTAGGAATAA+TGG - tig0023062:31414-31433 None:intergenic 30.0%
GTACTAAATGTACTCCAATT+AGG - tig0023062:31422-31441 None:intergenic 30.0%
TTGGAGTACATTTAGTACAA+TGG + tig0023062:31424-31443 Msa1404370:CDS 30.0%
AAACTCAGGAGATAATGAAA+AGG - tig0023062:31539-31558 None:intergenic 30.0%
ATTTATAGTTATTCCACACG+CGG - tig0023062:31595-31614 None:intergenic 30.0%
TTTCCAAGTATACCTAACTT+GGG + tig0023062:31620-31639 Msa1404370:CDS 30.0%
TATTTCTTCTCTCGTAAGAA+TGG + tig0023062:31655-31674 Msa1404370:CDS 30.0%
ATAAGCTGGTATCTTAAGAA+AGG + tig0023062:31761-31780 Msa1404370:CDS 30.0%
TTCTTTAAGCATATGAGTGA+AGG + tig0023062:31806-31825 Msa1404370:CDS 30.0%
CAACAACTCAATTAAAACCT+CGG - tig0023062:32023-32042 None:intergenic 30.0%
! AATTGAGTTGTTGAAGAAGT+CGG + tig0023062:32030-32049 Msa1404370:CDS 30.0%
ATTCTTTCAATCCTGTTTGA+CGG - tig0023062:32103-32122 None:intergenic 30.0%
ATCAAAAGCTACTATCTACT+TGG - tig0023062:32180-32199 None:intergenic 30.0%
! ACACCTTCAATTTGAAATGT+AGG - tig0023062:32215-32234 None:intergenic 30.0%
!! AATGAGTTGATAGAACATAG+GGG + tig0023062:32426-32445 Msa1404370:intron 30.0%
CTTATCACAACAGTTATGAA+GGG + tig0023062:32486-32505 Msa1404370:intron 30.0%
ATAGAAAACATACACAAGGA+TGG - tig0023062:32594-32613 None:intergenic 30.0%
AGTCATAGAAAACATACACA+AGG - tig0023062:32598-32617 None:intergenic 30.0%
! AAAGGGAAAGAGAAACGTTT+TGG - tig0023062:28942-28961 None:intergenic 35.0%
! ACATTGACCCTTTGTTTGAA+GGG - tig0023062:28985-29004 None:intergenic 35.0%
! AATGTGTTTTACACTGACAG+TGG + tig0023062:29000-29019 Msa1404370:CDS 35.0%
! TTTTACACTGACAGTGGAAT+TGG + tig0023062:29006-29025 Msa1404370:CDS 35.0%
ATACAAGAGGATTTCGATGA+TGG + tig0023062:29134-29153 Msa1404370:CDS 35.0%
! ATGATGGAGAATCCTGAATT+GGG + tig0023062:29150-29169 Msa1404370:CDS 35.0%
TGCTTCTGTGTTGAATCAAT+GGG + tig0023062:29176-29195 Msa1404370:CDS 35.0%
TTGAATCAATGGGAGAATGA+AGG + tig0023062:29186-29205 Msa1404370:CDS 35.0%
AAAACATGATAGAGCTCTTC+AGG + tig0023062:29266-29285 Msa1404370:CDS 35.0%
ATGGTTAAAACAACTGCAGT+AGG + tig0023062:29324-29343 Msa1404370:intron 35.0%
!!! CCTAGCTTTTTTGGTTTTTG+CGG + tig0023062:29347-29366 Msa1404370:intron 35.0%
!!! TGCTAGTTTTCGCTGTTTTA+GGG + tig0023062:29407-29426 Msa1404370:intron 35.0%
TGCTTCATAATGTTAAGGAG+GGG - tig0023062:29454-29473 None:intergenic 35.0%
GTTGCTTCATAATGTTAAGG+AGG - tig0023062:29456-29475 None:intergenic 35.0%
! GCTGTTGCTTCATAATGTTA+AGG - tig0023062:29459-29478 None:intergenic 35.0%
! ACCGTTCATTCTTACTCTTT+TGG - tig0023062:29516-29535 None:intergenic 35.0%
! TAACATGGTAGTGTATTGGA+TGG - tig0023062:29544-29563 None:intergenic 35.0%
TCTTTGGTACGATAGAGATT+GGG - tig0023062:29598-29617 None:intergenic 35.0%
TGTTGGACCATTCCATAATA+CGG + tig0023062:29627-29646 Msa1404370:intron 35.0%
TTGGTCATGATATAGTTCTC+AGG - tig0023062:29894-29913 None:intergenic 35.0%
TTGCCAAATAGATGACACTT+TGG + tig0023062:30173-30192 Msa1404370:intron 35.0%
! TAGCCAAAGTGTCATCTATT+TGG - tig0023062:30179-30198 None:intergenic 35.0%
TTTGGACACAGATTGAACAA+TGG - tig0023062:30309-30328 None:intergenic 35.0%
!! GACCAATACAATTTTTAGGG+TGG - tig0023062:30396-30415 None:intergenic 35.0%
!! GCCCCATAAAATTTTAACGT+TGG - tig0023062:30581-30600 None:intergenic 35.0%
! ACGTTAAAATTTTATGGGGC+CGG + tig0023062:30581-30600 Msa1404370:intron 35.0%
TTACTGACAGTTTGCAAATC+TGG - tig0023062:30758-30777 None:intergenic 35.0%
TAGTAGAATCATAGGGGAAA+TGG - tig0023062:30800-30819 None:intergenic 35.0%
TCCCCTATGATTCTACTAAA+CGG + tig0023062:30802-30821 Msa1404370:intron 35.0%
TCCGTTTAGTAGAATCATAG+GGG - tig0023062:30806-30825 None:intergenic 35.0%
CATACTTTCATGCATTCCAA+AGG - tig0023062:30835-30854 None:intergenic 35.0%
! GACTAATGAACATGTTTTGC+AGG + tig0023062:30941-30960 Msa1404370:intron 35.0%
TCGAAGTAATGAGGAGTAAA+AGG + tig0023062:31177-31196 Msa1404370:CDS 35.0%
TCAAGGATCAATAGAGAAGA+TGG + tig0023062:31355-31374 Msa1404370:CDS 35.0%
CTCCAATTAGGAATAATGGT+AGG - tig0023062:31410-31429 None:intergenic 35.0%
GGCTGCAATGTATATTAAGA+TGG + tig0023062:31445-31464 Msa1404370:CDS 35.0%
!! AGCACAAGAGTGCTTAAAAA+AGG + tig0023062:31481-31500 Msa1404370:CDS 35.0%
CATTATCTCCTGAGTTTGTA+TGG + tig0023062:31542-31561 Msa1404370:CDS 35.0%
GTTTCCAAGTATACCTAACT+TGG + tig0023062:31619-31638 Msa1404370:CDS 35.0%
TATCCCAAGTTAGGTATACT+TGG - tig0023062:31626-31645 None:intergenic 35.0%
GTAAGAATGGACGATATTGA+GGG + tig0023062:31668-31687 Msa1404370:CDS 35.0%
TAAGAATGGACGATATTGAG+GGG + tig0023062:31669-31688 Msa1404370:CDS 35.0%
AGAAAAGCTTTACGAGGAAT+GGG + tig0023062:31694-31713 Msa1404370:CDS 35.0%
!! CCTATTCTAGAATCATCAGA+GGG - tig0023062:31731-31750 None:intergenic 35.0%
!! TCCTATTCTAGAATCATCAG+AGG - tig0023062:31732-31751 None:intergenic 35.0%
TAAGATACCAGCTTATGAGA+AGG - tig0023062:31757-31776 None:intergenic 35.0%
TTTAAGCATATGAGTGAAGG+TGG + tig0023062:31809-31828 Msa1404370:CDS 35.0%
ATGTCCAAATTCGACTACAT+GGG + tig0023062:31835-31854 Msa1404370:CDS 35.0%
AGGGTCATATTGCTGAAAAT+AGG + tig0023062:31870-31889 Msa1404370:CDS 35.0%
GGGTCATATTGCTGAAAATA+GGG + tig0023062:31871-31890 Msa1404370:CDS 35.0%
GAAACCCAAGCCAATAAAAT+TGG + tig0023062:31952-31971 Msa1404370:CDS 35.0%
! AGGAATGCAGCCAATTTTAT+TGG - tig0023062:31965-31984 None:intergenic 35.0%
TTTCGTCTTGACAAAGCTTA+AGG - tig0023062:31985-32004 None:intergenic 35.0%
! ATTGAGTTGTTGAAGAAGTC+GGG + tig0023062:32031-32050 Msa1404370:CDS 35.0%
! TTGAAATGTAGGTGCTACAT+AGG - tig0023062:32204-32223 None:intergenic 35.0%
GCACCTACATTTCAAATTGA+AGG + tig0023062:32209-32228 Msa1404370:intron 35.0%
! CATTTCAAATTGAAGGTGTG+TGG + tig0023062:32216-32235 Msa1404370:intron 35.0%
TGTTGGTGTAATGTCAATGT+TGG - tig0023062:32262-32281 None:intergenic 35.0%
!! ATGAGTTGATAGAACATAGG+GGG + tig0023062:32427-32446 Msa1404370:intron 35.0%
GCTTATCACAACAGTTATGA+AGG + tig0023062:32485-32504 Msa1404370:intron 35.0%
! GACTGATGTTTTGCTCTAAT+AGG + tig0023062:32537-32556 Msa1404370:intron 35.0%
! ATTGACCCTTTGTTTGAAGG+GGG - tig0023062:28983-29002 None:intergenic 40.0%
! CATTGACCCTTTGTTTGAAG+GGG - tig0023062:28984-29003 None:intergenic 40.0%
! CACATTGACCCTTTGTTTGA+AGG - tig0023062:28986-29005 None:intergenic 40.0%
ACTGACAGTGGAATTGGAAT+TGG + tig0023062:29012-29031 Msa1404370:CDS 40.0%
!! GATGATGGAGAATCCTGAAT+TGG + tig0023062:29149-29168 Msa1404370:CDS 40.0%
CTGCTTCTGTGTTGAATCAA+TGG + tig0023062:29175-29194 Msa1404370:CDS 40.0%
ATCAATGGGAGAATGAAGGA+AGG + tig0023062:29190-29209 Msa1404370:CDS 40.0%
AAGGAAGGAATCTCACCAAA+TGG + tig0023062:29205-29224 Msa1404370:CDS 40.0%
AGGAAGGAATCTCACCAAAT+GGG + tig0023062:29206-29225 Msa1404370:CDS 40.0%
AACTCTGCAAAGTTCCCATT+TGG - tig0023062:29223-29242 None:intergenic 40.0%
CCGCAAAAACCAAAAAAGCT+AGG - tig0023062:29350-29369 None:intergenic 40.0%
!!! CTGCTAGTTTTCGCTGTTTT+AGG + tig0023062:29406-29425 Msa1404370:intron 40.0%
!!! GCTAGTTTTCGCTGTTTTAG+GGG + tig0023062:29408-29427 Msa1404370:intron 40.0%
!! GAGGGGGTATGTTTTTAACA+CGG - tig0023062:29437-29456 None:intergenic 40.0%
GCTTCATAATGTTAAGGAGG+GGG - tig0023062:29453-29472 None:intergenic 40.0%
! AGGCTAACATGGTAGTGTAT+TGG - tig0023062:29548-29567 None:intergenic 40.0%
GTCTTTGGTACGATAGAGAT+TGG - tig0023062:29599-29618 None:intergenic 40.0%
TCACCATCCGTATTATGGAA+TGG - tig0023062:29637-29656 None:intergenic 40.0%
GATGATCACCATCCGTATTA+TGG - tig0023062:29642-29661 None:intergenic 40.0%
! TCTATTTTCAGCAGGACAAC+AGG - tig0023062:29784-29803 None:intergenic 40.0%
! GTTTTTACTTTGGCTGATGG+AGG - tig0023062:29831-29850 None:intergenic 40.0%
!!! CCTGTTTTTACTTTGGCTGA+TGG - tig0023062:29834-29853 None:intergenic 40.0%
CCATCAGCCAAAGTAAAAAC+AGG + tig0023062:29831-29850 Msa1404370:intron 40.0%
!!! GGTTGCTCCTGTTTTTACTT+TGG - tig0023062:29841-29860 None:intergenic 40.0%
ATATCATGACCAAACTACCC+CGG + tig0023062:29901-29920 Msa1404370:intron 40.0%
!! GTTTATTTTCCGGGGTAGTT+TGG - tig0023062:29913-29932 None:intergenic 40.0%
! TTAACAGCTTTTGCAGTGAC+TGG + tig0023062:30007-30026 Msa1404370:intron 40.0%
! TTTGTGTGTGTGTTGCTGAT+TGG - tig0023062:30079-30098 None:intergenic 40.0%
! TAGGGTGGATTTCATAGTCT+TGG - tig0023062:30381-30400 None:intergenic 40.0%
TTAACGTTGGTGTGAGAACA+TGG - tig0023062:30568-30587 None:intergenic 40.0%
TACCACGTCAAAAGTTTAGC+CGG - tig0023062:30603-30622 None:intergenic 40.0%
! GCAAATCTGGAATTGACATG+TGG - tig0023062:30745-30764 None:intergenic 40.0%
ACTAAACGGAATGCTTCCTT+TGG + tig0023062:30816-30835 Msa1404370:intron 40.0%
GCAGATATTCTAAACCTCTC+TGG - tig0023062:30990-31009 None:intergenic 40.0%
GATCTAATCGCAAGAGTTCA+CGG + tig0023062:31032-31051 Msa1404370:CDS 40.0%
GACTCTGTACATGAACCTTA+AGG + tig0023062:31235-31254 Msa1404370:CDS 40.0%
AATATCTGGTTGTCCTCTTG+TGG + tig0023062:31329-31348 Msa1404370:CDS 40.0%
TCGACCTAATATTCTACCCT+CGG - tig0023062:31507-31526 None:intergenic 40.0%
CAACACTTCCATACAAACTC+AGG - tig0023062:31553-31572 None:intergenic 40.0%
! CTGAGTTTGTATGGAAGTGT+TGG + tig0023062:31551-31570 Msa1404370:CDS 40.0%
!! GTATGGAAGTGTTGGAAACA+AGG + tig0023062:31559-31578 Msa1404370:CDS 40.0%
CGAGAGAAGAAATAACAGCA+TGG - tig0023062:31649-31668 None:intergenic 40.0%
CGTAAGAATGGACGATATTG+AGG + tig0023062:31667-31686 Msa1404370:CDS 40.0%
AAGAATGGACGATATTGAGG+GGG + tig0023062:31670-31689 Msa1404370:CDS 40.0%
CAGAAAAGCTTTACGAGGAA+TGG + tig0023062:31693-31712 Msa1404370:CDS 40.0%
!! CCCTCTGATGATTCTAGAAT+AGG + tig0023062:31728-31747 Msa1404370:CDS 40.0%
TAGGAAACCTTCTCATAAGC+TGG + tig0023062:31747-31766 Msa1404370:CDS 40.0%
GATGTCCAAATTCGACTACA+TGG + tig0023062:31834-31853 Msa1404370:CDS 40.0%
TGCTGAAAATAGGGTTTCTG+AGG + tig0023062:31880-31899 Msa1404370:CDS 40.0%
CGACAAGTGATTCAAAGAGT+TGG + tig0023062:31930-31949 Msa1404370:CDS 40.0%
!!! GCAGCCAATTTTATTGGCTT+GGG - tig0023062:31959-31978 None:intergenic 40.0%
!! TGCAGCCAATTTTATTGGCT+TGG - tig0023062:31960-31979 None:intergenic 40.0%
TTGTCAAGACGAAAACGACA+TGG + tig0023062:31991-32010 Msa1404370:CDS 40.0%
CTAGGTTCTTATGCTAGGTT+AGG + tig0023062:32509-32528 Msa1404370:intron 40.0%
TCTTATGCTAGGTTAGGACA+TGG + tig0023062:32515-32534 Msa1404370:intron 40.0%
CTTCTGATGCATGTGATATG+AGG + tig0023062:32568-32587 Msa1404370:CDS 40.0%
TCTTCCCCCCTTCAAACAAA+GGG + tig0023062:28975-28994 Msa1404370:CDS 45.0%
! TTGACCCTTTGTTTGAAGGG+GGG - tig0023062:28982-29001 None:intergenic 45.0%
GACAGTGGAATTGGAATTGG+AGG + tig0023062:29015-29034 Msa1404370:CDS 45.0%
ACAGAAGCAGAACCCAATTC+AGG - tig0023062:29165-29184 None:intergenic 45.0%
!!! TGCAGTAGGCCTAGCTTTTT+TGG + tig0023062:29338-29357 Msa1404370:intron 45.0%
!!! TTGGTTTTTGCGGTGCTGTT+TGG + tig0023062:29357-29376 Msa1404370:intron 45.0%
CATCTACAGTGAGGCTAACA+TGG - tig0023062:29559-29578 None:intergenic 45.0%
CGTAGTTCACATCTACAGTG+AGG - tig0023062:29568-29587 None:intergenic 45.0%
TTGGTACGATAGAGATTGGG+AGG - tig0023062:29595-29614 None:intergenic 45.0%
ATGGAATGGTCCAACAACAG+TGG - tig0023062:29623-29642 None:intergenic 45.0%
GGACCATTCCATAATACGGA+TGG + tig0023062:29631-29650 Msa1404370:intron 45.0%
! AAGCCTTATCCCACTAAGTG+GGG - tig0023062:29679-29698 None:intergenic 45.0%
! CAAGCCTTATCCCACTAAGT+GGG - tig0023062:29680-29699 None:intergenic 45.0%
! GGAGGGATTCTATTTTCAGC+AGG - tig0023062:29792-29811 None:intergenic 45.0%
GGATGAACAATAGACCAGAG+AGG + tig0023062:30973-30992 Msa1404370:CDS 45.0%
TTGTCCTCTTGTGGATCTCA+AGG + tig0023062:31338-31357 Msa1404370:CDS 45.0%
TGATCCTTGAGATCCACAAG+AGG - tig0023062:31345-31364 None:intergenic 45.0%
! AGAGTGCTTAAAAAAGGCCG+AGG + tig0023062:31487-31506 Msa1404370:CDS 45.0%
GAGTGCTTAAAAAAGGCCGA+GGG + tig0023062:31488-31507 Msa1404370:CDS 45.0%
AAGGCCGAGGGTAGAATATT+AGG + tig0023062:31500-31519 Msa1404370:CDS 45.0%
ACAGCATGGTATCCCAAGTT+AGG - tig0023062:31635-31654 None:intergenic 45.0%
! ACGAGGAATGGGTTTCTGTA+AGG + tig0023062:31705-31724 Msa1404370:CDS 45.0%
AAGCATATGAGTGAAGGTGG+TGG + tig0023062:31812-31831 Msa1404370:CDS 45.0%
AGCTCCCATGTAGTCGAATT+TGG - tig0023062:31842-31861 None:intergenic 45.0%
TACATGGGAGCTTCTTTCTG+AGG + tig0023062:31850-31869 Msa1404370:CDS 45.0%
ACATGGGAGCTTCTTTCTGA+GGG + tig0023062:31851-31870 Msa1404370:CDS 45.0%
GCAAAGATGAACCGTCAAAC+AGG + tig0023062:32089-32108 Msa1404370:CDS 45.0%
GTGTGGTGTCTAACATGTGT+CGG + tig0023062:32233-32252 Msa1404370:intron 45.0%
CACAACAGTTATGAAGGGCT+AGG + tig0023062:32491-32510 Msa1404370:intron 45.0%
AAGGGCTAGGTTCTTATGCT+AGG + tig0023062:32504-32523 Msa1404370:intron 45.0%
!!! TATAAATTTTTTAATTCAAA+AGG + tig0023062:30534-30553 Msa1404370:intron 5.0%
CTCTTCCCCCCTTCAAACAA+AGG + tig0023062:28974-28993 Msa1404370:CDS 50.0%
ACCAAAGACGCCACTGTTGT+TGG + tig0023062:29610-29629 Msa1404370:intron 50.0%
TCCAACAACAGTGGCGTCTT+TGG - tig0023062:29614-29633 None:intergenic 50.0%
TGTTATCCGACCCCACTTAG+TGG + tig0023062:29666-29685 Msa1404370:intron 50.0%
GTTATCCGACCCCACTTAGT+GGG + tig0023062:29667-29686 Msa1404370:intron 50.0%
CTTATCCCACTAAGTGGGGT+CGG - tig0023062:29675-29694 None:intergenic 50.0%
! CCAAGCCTTATCCCACTAAG+TGG - tig0023062:29681-29700 None:intergenic 50.0%
CCACTTAGTGGGATAAGGCT+TGG + tig0023062:29678-29697 Msa1404370:intron 50.0%
CTCCACCAGAACTGCTTCAT+TGG + tig0023062:29758-29777 Msa1404370:intron 50.0%
ACAGGCCAATGAAGCAGTTC+TGG - tig0023062:29766-29785 None:intergenic 50.0%
! ACTTTGGCTGATGGAGGCAT+CGG - tig0023062:29825-29844 None:intergenic 50.0%
GTCAGTGGTGACTCAGTTGA+TGG + tig0023062:30413-30432 Msa1404370:intron 50.0%
ACGTTGGTGTGAGAACATGG+AGG - tig0023062:30565-30584 None:intergenic 50.0%
!! CACGGAGTTTCTAGTGCAGA+AGG + tig0023062:31050-31069 Msa1404370:CDS 50.0%
CCATAACGACGAAGCGTATG+CGG + tig0023062:32057-32076 Msa1404370:CDS 50.0%
TCCTCGATGTGTGTGTGTGT+GGG + tig0023062:32359-32378 Msa1404370:intron 50.0%
CGACCCCACTTAGTGGGATA+AGG + tig0023062:29673-29692 Msa1404370:intron 55.0%
! GGCCAATGAAGCAGTTCTGG+TGG - tig0023062:29763-29782 None:intergenic 55.0%
!! GCATCGGTGACTGGTTTGGA+GGG - tig0023062:29809-29828 None:intergenic 55.0%
! GGCCGGCTAAACTTTTGACG+TGG + tig0023062:30598-30617 Msa1404370:intron 55.0%
AGGACGAAGTTTACCGCGTG+TGG + tig0023062:31579-31598 Msa1404370:CDS 55.0%
GGGGGCAGAAAAGCTTTACG+AGG + tig0023062:31688-31707 Msa1404370:CDS 55.0%
!! TTCTGAGGCGCTTTCGTGCT+TGG + tig0023062:31895-31914 Msa1404370:CDS 55.0%
CCGCATACGCTTCGTCGTTA+TGG - tig0023062:32060-32079 None:intergenic 55.0%
GTCCTCGATGTGTGTGTGTG+TGG + tig0023062:32358-32377 Msa1404370:intron 55.0%
ACCCACACACACACACATCG+AGG - tig0023062:32363-32382 None:intergenic 55.0%
!! GGCATCGGTGACTGGTTTGG+AGG - tig0023062:29810-29829 None:intergenic 60.0%
!! GGAGGCATCGGTGACTGGTT+TGG - tig0023062:29813-29832 None:intergenic 60.0%
!! CTGATGGAGGCATCGGTGAC+TGG - tig0023062:29818-29837 None:intergenic 60.0%
!! AAACGACATGGAGAGCGCCG+AGG + tig0023062:32003-32022 Msa1404370:CDS 60.0%
Chromosome Type Strat End Strand Name
tig0023062 gene 28849 32644 28849 ID=Msa1404370;Name=Msa1404370
tig0023062 mRNA 28849 32644 28849 ID=Msa1404370-mRNA-1;Parent=Msa1404370;Name=Msa1404370-mRNA-1;_AED=0.15;_eAED=0.15;_QI=43|0.5|0.33|1|1|1|3|0|551
tig0023062 exon 28849 29287 28849 ID=Msa1404370-mRNA-1:exon:19768;Parent=Msa1404370-mRNA-1
tig0023062 exon 30963 32136 30963 ID=Msa1404370-mRNA-1:exon:19769;Parent=Msa1404370-mRNA-1
tig0023062 exon 32559 32644 32559 ID=Msa1404370-mRNA-1:exon:19770;Parent=Msa1404370-mRNA-1
tig0023062 five_prime_UTR 28849 28891 28849 ID=Msa1404370-mRNA-1:five_prime_utr;Parent=Msa1404370-mRNA-1
tig0023062 CDS 28892 29287 28892 ID=Msa1404370-mRNA-1:cds;Parent=Msa1404370-mRNA-1
tig0023062 CDS 30963 32136 30963 ID=Msa1404370-mRNA-1:cds;Parent=Msa1404370-mRNA-1
tig0023062 CDS 32559 32644 32559 ID=Msa1404370-mRNA-1:cds;Parent=Msa1404370-mRNA-1
Gene Sequence

>Msa1404370

ATGATGACTACTATTACCCCTCTTCAAATTCAATCTTCTTTACAAAACCAAAACGTTTCTCTTTCCCTTTCTTATTCTCATACTCTTCCCCCCTTCAAACAAAGGGTCAATGTGTTTTACACTGACAGTGGAATTGGAATTGGAGGAGCAAGAAGAGTTTCTGTCACTTGTTCAATCTCAAAGATTCACAGTTATGGTACACTTGATTATGAGAGAAAACCCAATTTGAAATGGAATGCTATATACAAGAGGATTTCGATGATGGAGAATCCTGAATTGGGTTCTGCTTCTGTGTTGAATCAATGGGAGAATGAAGGAAGGAATCTCACCAAATGGGAACTTTGCAGAGTTGTTAAAGAGTTGAGAAAGTACAGAAAACATGATAGAGCTCTTCAGGTATATGATTGGATGAACAATAGACCAGAGAGGTTTAGAATATCTGCAAGTGACGTTGCGATTCAGTTAGATCTAATCGCAAGAGTTCACGGAGTTTCTAGTGCAGAAGGTTTTTTTCTGAACCTAACCAATGATTTAAAAGACAAGAGAACATATGGTGCCCTCTTGAATGCATATGTTCATTCTAGATCAAGAGAAAAGGCAGAATCTTTACTCGAAGTAATGAGGAGTAAAAGGTACTTAATACATTCACTACCGTTTAATTTAATGATGACTCTGTACATGAACCTTAAGGATTATGATAAAGTCGATATGTTGGTTTCAGAAATGAAAGAAAAGAACATACAGCTAGATATATATACATATAATATCTGGTTGTCCTCTTGTGGATCTCAAGGATCAATAGAGAAGATGGAACAAGTTTTTGAACAGATGTCAAAGGATCCTACCATTATTCCTAATTGGAGTACATTTAGTACAATGGCTGCAATGTATATTAAGATGGAACTGTTTGAAAAAGCACAAGAGTGCTTAAAAAAGGCCGAGGGTAGAATATTAGGTCGAGATAAAGTACCTTTTCATTATCTCCTGAGTTTGTATGGAAGTGTTGGAAACAAGGACGAAGTTTACCGCGTGTGGAATAACTATAAATCAATGTTTCCAAGTATACCTAACTTGGGATACCATGCTGTTATTTCTTCTCTCGTAAGAATGGACGATATTGAGGGGGCAGAAAAGCTTTACGAGGAATGGGTTTCTGTAAGGCCCTCTGATGATTCTAGAATAGGAAACCTTCTCATAAGCTGGTATCTTAAGAAAGGTAAATCAGATAAAGTTTTTAGCTTCTTTAAGCATATGAGTGAAGGTGGTGGATGTCCAAATTCGACTACATGGGAGCTTCTTTCTGAGGGTCATATTGCTGAAAATAGGGTTTCTGAGGCGCTTTCGTGCTTGGAAAAAGCTTTTACGACAAGTGATTCAAAGAGTTGGAAACCCAAGCCAATAAAATTGGCTGCATTCCTTAAGCTTTGTCAAGACGAAAACGACATGGAGAGCGCCGAGGTTTTAATTGAGTTGTTGAAGAAGTCGGGATACCATAACGACGAAGCGTATGCGGCACTCATTAGCAAAGATGAACCGTCAAACAGGATTGAAAGAATTGATGATGTTGACAGAATTGACTCTTCTGATGCATGTGATATGAGGCCATCCTTGTGTATGTTTTCTATGACTTGTGTCTTTAAATTTGGAATGAAGTAG
Protein sequence

>Msa1404370

MMTTITPLQIQSSLQNQNVSLSLSYSHTLPPFKQRVNVFYTDSGIGIGGARRVSVTCSISKIHSYGTLDYERKPNLKWNAIYKRISMMENPELGSASVLNQWENEGRNLTKWELCRVVKELRKYRKHDRALQVYDWMNNRPERFRISASDVAIQLDLIARVHGVSSAEGFFLNLTNDLKDKRTYGALLNAYVHSRSREKAESLLEVMRSKRYLIHSLPFNLMMTLYMNLKDYDKVDMLVSEMKEKNIQLDIYTYNIWLSSCGSQGSIEKMEQVFEQMSKDPTIIPNWSTFSTMAAMYIKMELFEKAQECLKKAEGRILGRDKVPFHYLLSLYGSVGNKDEVYRVWNNYKSMFPSIPNLGYHAVISSLVRMDDIEGAEKLYEEWVSVRPSDDSRIGNLLISWYLKKGKSDKVFSFFKHMSEGGGCPNSTTWELLSEGHIAENRVSEALSCLEKAFTTSDSKSWKPKPIKLAAFLKLCQDENDMESAEVLIELLKKSGYHNDEAYAALISKDEPSNRIERIDDVDRIDSSDACDMRPSLCMFSMTCVFKFGMK*