Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0472820 | XP_013461511.2 | 96.172 | 209 | 8 | 0 | 1 | 209 | 1 | 209 | 4.88e-132 | 379 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0472820 | sp|A0A2U7XUE3|CHIL2_HUMLU | 70.335 | 209 | 62 | 0 | 1 | 209 | 1 | 209 | 3.18e-97 | 284 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0472820 | I3S971 | 96.172 | 209 | 8 | 0 | 1 | 209 | 1 | 209 | 2.33e-132 | 379 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0015070 | Msa0472820 | 0.813247 | 2.819848e-51 | -8.615850e-47 |
| Msa0024650 | Msa0472820 | 0.804773 | 1.839142e-49 | -8.615850e-47 |
| Msa0025570 | Msa0472820 | 0.802257 | 6.113128e-49 | -8.615850e-47 |
| Msa0051910 | Msa0472820 | 0.822573 | 2.203831e-53 | -8.615850e-47 |
| Msa0072440 | Msa0472820 | 0.818971 | 1.483803e-52 | -8.615850e-47 |
| Msa0107830 | Msa0472820 | 0.827702 | 1.352400e-54 | -8.615850e-47 |
| Msa0111780 | Msa0472820 | 0.819769 | 9.761898e-53 | -8.615850e-47 |
| Msa0120860 | Msa0472820 | 0.836793 | 7.597105e-57 | -8.615850e-47 |
| Msa0121320 | Msa0472820 | 0.801158 | 1.027524e-48 | -8.615850e-47 |
| Msa0125860 | Msa0472820 | 0.818640 | 1.763496e-52 | -8.615850e-47 |
| Msa0129290 | Msa0472820 | 0.801835 | 7.466248e-49 | -8.615850e-47 |
| Msa0137760 | Msa0472820 | 0.800888 | 1.166701e-48 | -8.615850e-47 |
| Msa0145050 | Msa0472820 | 0.812750 | 3.623161e-51 | -8.615850e-47 |
| Msa0163690 | Msa0472820 | 0.807007 | 6.235735e-50 | -8.615850e-47 |
| Msa0166180 | Msa0472820 | 0.801711 | 7.915714e-49 | -8.615850e-47 |
| Msa0187660 | Msa0472820 | 0.806948 | 6.419332e-50 | -8.615850e-47 |
| Msa0220090 | Msa0472820 | 0.809064 | 2.275701e-50 | -8.615850e-47 |
| Msa0374470 | Msa0472820 | 0.800434 | 1.443915e-48 | -8.615850e-47 |
| Msa0374600 | Msa0472820 | 0.803685 | 3.098103e-49 | -8.615850e-47 |
| Msa0378320 | Msa0472820 | 0.801025 | 1.093695e-48 | -8.615850e-47 |
| Msa0390200 | Msa0472820 | 0.824726 | 6.905031e-54 | -8.615850e-47 |
| Msa0405200 | Msa0472820 | 0.805712 | 1.169148e-49 | -8.615850e-47 |
| Msa0405210 | Msa0472820 | 0.819782 | 9.692079e-53 | -8.615850e-47 |
| Msa0433090 | Msa0472820 | 0.805722 | 1.163603e-49 | -8.615850e-47 |
| Msa0449500 | Msa0472820 | 0.806178 | 9.330035e-50 | -8.615850e-47 |
| Msa0460950 | Msa0472820 | 0.803258 | 3.798993e-49 | -8.615850e-47 |
| Msa0470420 | Msa0472820 | 0.812154 | 4.889631e-51 | -8.615850e-47 |
| Msa0472820 | Msa0490100 | 0.804357 | 2.245977e-49 | -8.615850e-47 |
| Msa0472820 | Msa0493870 | 0.806428 | 8.267165e-50 | -8.615850e-47 |
| Msa0472820 | Msa0497680 | 0.815425 | 9.304768e-52 | -8.615850e-47 |
| Msa0472820 | Msa0524710 | 0.808339 | 3.250775e-50 | -8.615850e-47 |
| Msa0472820 | Msa0525400 | 0.800002 | 1.767424e-48 | -8.615850e-47 |
| Msa0472820 | Msa0526880 | 0.801192 | 1.011268e-48 | -8.615850e-47 |
| Msa0472820 | Msa0580380 | 0.820070 | 8.328173e-53 | -8.615850e-47 |
| Msa0472820 | Msa0583390 | 0.813910 | 2.014775e-51 | -8.615850e-47 |
| Msa0472820 | Msa0588040 | 0.808549 | 2.932516e-50 | -8.615850e-47 |
| Msa0472820 | Msa0597170 | 0.823064 | 1.694003e-53 | -8.615850e-47 |
| Msa0472820 | Msa0597820 | 0.828095 | 1.087471e-54 | -8.615850e-47 |
| Msa0472820 | Msa0606600 | 0.804766 | 1.845108e-49 | -8.615850e-47 |
| Msa0472820 | Msa0614020 | -0.802880 | 4.547840e-49 | -8.615850e-47 |
| Msa0472820 | Msa0614960 | 0.808962 | 2.393367e-50 | -8.615850e-47 |
| Msa0472820 | Msa0645060 | 0.808538 | 2.947873e-50 | -8.615850e-47 |
| Msa0472820 | Msa0652590 | 0.806545 | 7.809491e-50 | -8.615850e-47 |
| Msa0472820 | Msa0675620 | 0.828986 | 6.627438e-55 | -8.615850e-47 |
| Msa0472820 | Msa0686830 | 0.803440 | 3.482090e-49 | -8.615850e-47 |
| Msa0472820 | Msa0699170 | 0.818505 | 1.892967e-52 | -8.615850e-47 |
| Msa0472820 | Msa0716620 | 0.810816 | 9.551213e-51 | -8.615850e-47 |
| Msa0472820 | Msa0731580 | 0.814645 | 1.386133e-51 | -8.615850e-47 |
| Msa0472820 | Msa0733560 | 0.800333 | 1.513704e-48 | -8.615850e-47 |
| Msa0472820 | Msa0744710 | 0.809398 | 1.929790e-50 | -8.615850e-47 |
| Msa0472820 | Msa0774660 | 0.807857 | 4.118188e-50 | -8.615850e-47 |
| Msa0472820 | Msa0794200 | 0.807217 | 5.629304e-50 | -8.615850e-47 |
| Msa0472820 | Msa0795880 | 0.807428 | 5.079911e-50 | -8.615850e-47 |
| Msa0472820 | Msa0799120 | 0.814852 | 1.247294e-51 | -8.615850e-47 |
| Msa0472820 | Msa0809920 | 0.831852 | 1.319408e-55 | -8.615850e-47 |
| Msa0472820 | Msa0814160 | 0.805014 | 1.637620e-49 | -8.615850e-47 |
| Msa0472820 | Msa0819150 | 0.801638 | 8.192874e-49 | -8.615850e-47 |
| Msa0472820 | Msa0826240 | 0.814563 | 1.445396e-51 | -8.615850e-47 |
| Msa0472820 | Msa0829990 | 0.806095 | 9.713626e-50 | -8.615850e-47 |
| Msa0472820 | Msa0830930 | 0.837064 | 6.479035e-57 | -8.615850e-47 |
| Msa0472820 | Msa0854840 | 0.809521 | 1.816215e-50 | -8.615850e-47 |
| Msa0472820 | Msa0869660 | 0.802125 | 6.506569e-49 | -8.615850e-47 |
| Msa0472820 | Msa0874750 | 0.824381 | 8.324547e-54 | -8.615850e-47 |
| Msa0472820 | Msa0976400 | 0.816964 | 4.215758e-52 | -8.615850e-47 |
| Msa0472820 | Msa0985380 | 0.804802 | 1.813740e-49 | -8.615850e-47 |
| Msa0472820 | Msa1002820 | 0.801885 | 7.291560e-49 | -8.615850e-47 |
| Msa0472820 | Msa1035050 | 0.808582 | 2.885572e-50 | -8.615850e-47 |
| Msa0472820 | Msa1041260 | 0.808213 | 3.459202e-50 | -8.615850e-47 |
| Msa0472820 | Msa1056890 | 0.805417 | 1.348197e-49 | -8.615850e-47 |
| Msa0472820 | Msa1061840 | 0.815788 | 7.725976e-52 | -8.615850e-47 |
| Msa0472820 | Msa1062070 | 0.830167 | 3.421194e-55 | -8.615850e-47 |
| Msa0472820 | Msa1066150 | 0.827291 | 1.696980e-54 | -8.615850e-47 |
| Msa0472820 | Msa1068780 | 0.817189 | 3.752216e-52 | -8.615850e-47 |
| Msa0472820 | Msa1071350 | 0.804877 | 1.749155e-49 | -8.615850e-47 |
| Msa0472820 | Msa1075090 | 0.801028 | 1.092167e-48 | -8.615850e-47 |
| Msa0472820 | Msa1085820 | 0.807943 | 3.948991e-50 | -8.615850e-47 |
| Msa0472820 | Msa1108560 | 0.835786 | 1.369828e-56 | -8.615850e-47 |
| Msa0472820 | Msa1110900 | 0.813498 | 2.482566e-51 | -8.615850e-47 |
| Msa0472820 | Msa1127840 | 0.800048 | 1.730091e-48 | -8.615850e-47 |
| Msa0472820 | Msa1131770 | 0.810276 | 1.249072e-50 | -8.615850e-47 |
| Msa0472820 | Msa1132850 | 0.811472 | 6.883254e-51 | -8.615850e-47 |
| Msa0472820 | Msa1137750 | 0.806145 | 9.484512e-50 | -8.615850e-47 |
| Msa0472820 | Msa1150750 | 0.823582 | 1.281899e-53 | -8.615850e-47 |
| Msa0472820 | Msa1156740 | 0.824195 | 9.207835e-54 | -8.615850e-47 |
| Msa0472820 | Msa1165660 | 0.822819 | 1.931785e-53 | -8.615850e-47 |
| Msa0472820 | Msa1172870 | 0.806831 | 6.796022e-50 | -8.615850e-47 |
| Msa0472820 | Msa1179340 | 0.818228 | 2.187201e-52 | -8.615850e-47 |
| Msa0472820 | Msa1186430 | 0.807991 | 3.856582e-50 | -8.615850e-47 |
| Msa0472820 | Msa1202110 | 0.800251 | 1.573211e-48 | -8.615850e-47 |
| Msa0472820 | Msa1223960 | 0.827529 | 1.487742e-54 | -8.615850e-47 |
| Msa0472820 | Msa1223990 | 0.808522 | 2.971440e-50 | -8.615850e-47 |
| Msa0472820 | Msa1225890 | 0.810093 | 1.368214e-50 | -8.615850e-47 |
| Msa0472820 | Msa1240710 | 0.820271 | 7.489348e-53 | -8.615850e-47 |
| Msa0472820 | Msa1263110 | 0.828892 | 6.983462e-55 | -8.615850e-47 |
| Msa0472820 | Msa1269200 | 0.808911 | 2.454369e-50 | -8.615850e-47 |
| Msa0472820 | Msa1275220 | 0.802700 | 4.954478e-49 | -8.615850e-47 |
| Msa0472820 | Msa1279750 | 0.803137 | 4.023039e-49 | -8.615850e-47 |
| Msa0472820 | Msa1280540 | 0.813699 | 2.242529e-51 | -8.615850e-47 |
| Msa0472820 | Msa1343980 | 0.914586 | 1.610746e-84 | -8.615850e-47 |
| Msa0472820 | Msa1364690 | 0.807903 | 4.026953e-50 | -8.615850e-47 |
| Msa0472820 | Msa1366470 | 0.811222 | 7.800406e-51 | -8.615850e-47 |
| Msa0472820 | Msa1382740 | 0.800924 | 1.147159e-48 | -8.615850e-47 |
| Msa0472820 | Msa1386980 | 0.943379 | 1.339279e-102 | -8.615850e-47 |
| Msa0472820 | Msa1390200 | 0.812238 | 4.688854e-51 | -8.615850e-47 |
| Msa0472820 | Msa1437590 | 0.808761 | 2.641837e-50 | -8.615850e-47 |
| Msa0472820 | Msa1445010 | 0.808497 | 3.008496e-50 | -8.615850e-47 |
| Msa0472820 | Msa1458270 | 0.815109 | 1.093827e-51 | -8.615850e-47 |
| Msa0242720 | Msa0472820 | 0.827658 | 1.385488e-54 | -8.615850e-47 |
| Msa0261400 | Msa0472820 | 0.807620 | 4.623830e-50 | -8.615850e-47 |
| Msa0267700 | Msa0472820 | 0.801239 | 9.890254e-49 | -8.615850e-47 |
| Msa0271700 | Msa0472820 | 0.813834 | 2.094458e-51 | -8.615850e-47 |
| Msa0271720 | Msa0472820 | 0.810963 | 8.873688e-51 | -8.615850e-47 |
| Msa0303650 | Msa0472820 | 0.815180 | 1.054887e-51 | -8.615850e-47 |
| Msa0343520 | Msa0472820 | 0.800394 | 1.471459e-48 | -8.615850e-47 |
| Msa0346170 | Msa0472820 | 0.805031 | 1.624481e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0472820 | MtrunA17_Chr3g0128761 | 92.228 | 193 | 15 | 0 | 17 | 209 | 10 | 202 | 1.94e-117 | 332 |
| Msa0472820 | MtrunA17_Chr1g0213011 | 32.227 | 211 | 131 | 5 | 6 | 208 | 8 | 214 | 1.08e-20 | 86.3 |
| Msa0472820 | MtrunA17_Chr1g0213021 | 28.230 | 209 | 140 | 4 | 6 | 207 | 11 | 216 | 3.66e-19 | 82.0 |
| Msa0472820 | MtrunA17_Chr1g0213031 | 27.536 | 207 | 140 | 4 | 6 | 205 | 11 | 214 | 1.29e-18 | 80.5 |
| Msa0472820 | MtrunA17_Chr1g0213051 | 27.442 | 215 | 145 | 6 | 1 | 205 | 1 | 214 | 2.63e-17 | 77.4 |
| Msa0472820 | MtrunA17_Chr2g0313901 | 28.500 | 200 | 132 | 6 | 10 | 207 | 86 | 276 | 2.38e-16 | 75.5 |
| Msa0472820 | MtrunA17_Chr1g0213041 | 27.805 | 205 | 140 | 5 | 8 | 205 | 11 | 214 | 3.13e-16 | 74.3 |
| Msa0472820 | MtrunA17_Chr7g0224381 | 28.758 | 153 | 102 | 3 | 1 | 146 | 1 | 153 | 1.23e-14 | 68.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0472820 | AT5G05270.1 | 63.158 | 209 | 76 | 1 | 1 | 208 | 1 | 209 | 1.04e-84 | 250 |
| Msa0472820 | AT5G05270.2 | 63.158 | 209 | 76 | 1 | 1 | 208 | 1 | 209 | 1.04e-84 | 250 |
| Msa0472820 | AT3G55120.1 | 29.907 | 214 | 130 | 8 | 8 | 209 | 21 | 226 | 2.86e-16 | 75.1 |
| Msa0472820 | AT1G53520.1 | 26.344 | 186 | 131 | 4 | 23 | 207 | 106 | 286 | 1.17e-15 | 73.9 |
| Msa0472820 | AT5G66230.2 | 28.638 | 213 | 126 | 8 | 8 | 209 | 11 | 208 | 2.81e-14 | 71.2 |
| Msa0472820 | AT5G66220.1 | 28.387 | 155 | 103 | 3 | 59 | 209 | 9 | 159 | 7.61e-13 | 64.7 |
Find 78 sgRNAs with CRISPR-Local
Find 115 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTATTCAATTGGGGTTTATT+TGG | 0.127513 | 3_3:+75256236 | Msa0472820:CDS |
| AGAATTTCACTTGGAGAAAA+TGG | 0.134571 | 3_3:-75256215 | None:intergenic |
| TTCTTTGACGCTCTCATTTC+TGG | 0.168815 | 3_3:+75256330 | Msa0472820:CDS |
| TGTTGTGATTAAAGAGATTA+AGG | 0.213746 | 3_3:+75256579 | Msa0472820:CDS |
| TCCTTCAACCATTACATCTT+TGG | 0.255062 | 3_3:+75257192 | Msa0472820:CDS |
| ATTGCTTAGGTTGTTATATA+AGG | 0.261912 | 3_3:-75255606 | None:intergenic |
| AATAAATTGTTATTCTTTGT+TGG | 0.283400 | 3_3:+75257400 | Msa0472820:three_prime_UTR |
| CAAGTGAAATTCTATTCAAT+TGG | 0.286514 | 3_3:+75256225 | Msa0472820:CDS |
| AATACAGATTGTGGTATCTT+TGG | 0.300414 | 3_3:+75257078 | Msa0472820:intron |
| TGCAAGTGGCTTGCTACTTC+AGG | 0.308776 | 3_3:-75256261 | None:intergenic |
| CACACTCCGTAGCTAAATTA+AGG | 0.334675 | 3_3:-75255571 | None:intergenic |
| AAGTGAAATTCTATTCAATT+GGG | 0.340514 | 3_3:+75256226 | Msa0472820:CDS |
| AGTGGCTAGTGAAACTGTTT+TGG | 0.373529 | 3_3:+75255848 | Msa0472820:intron |
| CATTGCACGTGTGGAATCTA+TGG | 0.382464 | 3_3:-75257429 | None:intergenic |
| GAGATTAAGGGTGCACAATA+TGG | 0.383753 | 3_3:+75256592 | Msa0472820:CDS |
| GGATGAAGAAGAAGAAGCTT+TGG | 0.390087 | 3_3:+75256669 | Msa0472820:CDS |
| GACTTTCTCCACTTTGTTTG+CGG | 0.390887 | 3_3:+75257311 | Msa0472820:three_prime_UTR |
| AGCTCTTACTAGTTAGTTGC+TGG | 0.395872 | 3_3:-75255721 | None:intergenic |
| TTGTCTTCCAACTCCTTGGC+AGG | 0.403272 | 3_3:-75256300 | None:intergenic |
| TCTGCAGTTGGAGAGTTTGC+TGG | 0.409543 | 3_3:-75256757 | None:intergenic |
| ATTAAGAAATGGTATCTTGC+TGG | 0.410454 | 3_3:+75257154 | Msa0472820:CDS |
| ACCAAGGAGAGAAAGGGTTT+TGG | 0.417350 | 3_3:-75255907 | None:intergenic |
| GACCGCTGTGAGGGACCGTT+TGG | 0.422176 | 3_3:+75256627 | Msa0472820:CDS |
| ACCAAAACCCTTTCTCTCCT+TGG | 0.424712 | 3_3:+75255906 | Msa0472820:CDS |
| TTGGTAGTAGTGATCTTTGA+AGG | 0.439710 | 3_3:-75255888 | None:intergenic |
| TGCTACTTTACCTCTGCAGT+TGG | 0.445412 | 3_3:-75256769 | None:intergenic |
| GTTGTGATTAAAGAGATTAA+GGG | 0.453545 | 3_3:+75256580 | Msa0472820:CDS |
| TAGAGAGAATTGTGGTGAGA+TGG | 0.457933 | 3_3:-75255670 | None:intergenic |
| TTCATCCTCATATTTGTCAT+CGG | 0.460901 | 3_3:-75256653 | None:intergenic |
| ATCATTGTCTTCCAACTCCT+TGG | 0.461782 | 3_3:-75256304 | None:intergenic |
| GTTTCTTGAAGTACTTAGAC+TGG | 0.476418 | 3_3:-75256711 | None:intergenic |
| TAGTGGAGACTATTAAGAAA+TGG | 0.482123 | 3_3:+75257143 | Msa0472820:CDS |
| TACCATGACCAAGGAGAGAA+AGG | 0.488761 | 3_3:-75255914 | None:intergenic |
| CTGCAGTTGGAGAGTTTGCT+GGG | 0.497551 | 3_3:-75256756 | None:intergenic |
| CTTAATAGTCTCCACTACAT+TGG | 0.499125 | 3_3:-75257137 | None:intergenic |
| GCCAAAGATGTAATGGTTGA+AGG | 0.506201 | 3_3:-75257193 | None:intergenic |
| CTTAAGATGAGATGTAAGAA+TGG | 0.508372 | 3_3:-75255634 | None:intergenic |
| TGGATAATTCCTCTGAGAGA+TGG | 0.511898 | 3_3:-75257219 | None:intergenic |
| CAATTGAATAGAATTTCACT+TGG | 0.511986 | 3_3:-75256224 | None:intergenic |
| AAAGTTTGCCATTGCACGTG+TGG | 0.515900 | 3_3:-75257438 | None:intergenic |
| AAGAATGGTAGATATTGCTT+AGG | 0.517256 | 3_3:-75255619 | None:intergenic |
| AGTCTAATTGCCTTCTCCAC+AGG | 0.518956 | 3_3:-75256556 | None:intergenic |
| ACTTGTGACTTTCTTTCAAG+TGG | 0.521102 | 3_3:-75255525 | None:intergenic |
| CAGATTGTGGTATCTTTGGA+AGG | 0.521938 | 3_3:+75257082 | Msa0472820:intron |
| TACCTTTCCACTGTTGCAAG+TGG | 0.522349 | 3_3:-75256275 | None:intergenic |
| ATGTGCAGCTCCTGTGGAGA+AGG | 0.525875 | 3_3:+75256546 | Msa0472820:intron |
| AGAGGAAGTAGAGAGAATTG+TGG | 0.528018 | 3_3:-75255678 | None:intergenic |
| ATGGGTAGCCAAAGATGTAA+TGG | 0.528630 | 3_3:-75257200 | None:intergenic |
| GGATAATTCCTCTGAGAGAT+GGG | 0.538139 | 3_3:-75257218 | None:intergenic |
| ACCATGACCAAGGAGAGAAA+GGG | 0.540135 | 3_3:-75255913 | None:intergenic |
| ACCCTTTCTCTCCTTGGTCA+TGG | 0.540415 | 3_3:+75255912 | Msa0472820:CDS |
| GTGAATATGTGCAGCTCCTG+TGG | 0.541590 | 3_3:+75256540 | Msa0472820:intron |
| AATGGTATCTTGCTGGCTCA+AGG | 0.546466 | 3_3:+75257161 | Msa0472820:CDS |
| CTACCACCTTAATTTAGCTA+CGG | 0.556695 | 3_3:+75255565 | Msa0472820:five_prime_UTR |
| CATAGATTCCACACGTGCAA+TGG | 0.557143 | 3_3:+75257430 | Msa0472820:three_prime_UTR |
| ATTTGTCATCGGCTGCCAAA+CGG | 0.557673 | 3_3:-75256642 | None:intergenic |
| AGTGAAATTCTATTCAATTG+GGG | 0.558547 | 3_3:+75256227 | Msa0472820:CDS |
| TGGTAGTAGTGATCTTTGAA+GGG | 0.566326 | 3_3:-75255887 | None:intergenic |
| ATGGTATCTTGCTGGCTCAA+GGG | 0.567607 | 3_3:+75257162 | Msa0472820:CDS |
| AGGTAAGCCTGCCAAGGAGT+TGG | 0.569479 | 3_3:+75256293 | Msa0472820:CDS |
| TAGCAAGCCACTTGCAACAG+TGG | 0.573061 | 3_3:+75256268 | Msa0472820:CDS |
| GGCAGCCGATGACAAATATG+AGG | 0.581635 | 3_3:+75256648 | Msa0472820:CDS |
| CGTAGCTAAATTAAGGTGGT+AGG | 0.585339 | 3_3:-75255564 | None:intergenic |
| GCAGAATTCCGCAAACAAAG+TGG | 0.588511 | 3_3:-75257319 | None:intergenic |
| TTTGATAGGGATTACCGACA+TGG | 0.591441 | 3_3:+75256188 | Msa0472820:intron |
| ATCGATGTAGATAGAGACAG+AGG | 0.592514 | 3_3:-75255696 | None:intergenic |
| AGCCACTTGCAACAGTGGAA+AGG | 0.600159 | 3_3:+75256273 | Msa0472820:CDS |
| AGATTGTGGTATCTTTGGAA+GGG | 0.601655 | 3_3:+75257083 | Msa0472820:intron |
| GTGGAAAGGTAAGCCTGCCA+AGG | 0.623835 | 3_3:+75256287 | Msa0472820:CDS |
| GAGAAAATGGATCTCCATGT+CGG | 0.629435 | 3_3:-75256202 | None:intergenic |
| TCAGATTGAGACCGCTGTGA+GGG | 0.629678 | 3_3:+75256618 | Msa0472820:CDS |
| AGCAAACTCTCCAACTGCAG+AGG | 0.645259 | 3_3:+75256759 | Msa0472820:CDS |
| AATAGAGAATGCCAATGTAG+TGG | 0.651147 | 3_3:+75257126 | Msa0472820:CDS |
| TGCCAAACGGTCCCTCACAG+CGG | 0.689378 | 3_3:-75256629 | None:intergenic |
| TTTGGCTACCCATCTCTCAG+AGG | 0.689828 | 3_3:+75257210 | Msa0472820:CDS |
| TTCAGATTGAGACCGCTGTG+AGG | 0.696200 | 3_3:+75256617 | Msa0472820:CDS |
| ACTCCGTAGCTAAATTAAGG+TGG | 0.699822 | 3_3:-75255568 | None:intergenic |
| GTAGCAACTTACCATGACCA+AGG | 0.712424 | 3_3:-75255923 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTGTTTAATAATAATGT+TGG | - | chr3_3:75255497-75255516 | None:intergenic | 10.0% |
| !! | CAACATTTATGATATAATTA+AGG | - | chr3_3:75256486-75256505 | None:intergenic | 15.0% |
| !!! | AATTAGTTTAAGTTGATTTT+AGG | + | chr3_3:75256839-75256858 | Msa0472820:intron | 15.0% |
| !!! | AAAAAAATTAAGTTGGTTAT+AGG | - | chr3_3:75256905-75256924 | None:intergenic | 15.0% |
| !!! | AATTCTGCTTTTTAATTTAT+TGG | + | chr3_3:75257334-75257353 | Msa0472820:three_prime_UTR | 15.0% |
| !!! | AATAAATTGTTATTCTTTGT+TGG | + | chr3_3:75257400-75257419 | Msa0472820:three_prime_UTR | 15.0% |
| !! | TCAAATCAAGAAAAAGAATT+TGG | - | chr3_3:75255977-75255996 | None:intergenic | 20.0% |
| !!! | TCTTGATTTGATTTTGAATA+TGG | + | chr3_3:75255986-75256005 | Msa0472820:intron | 20.0% |
| !!! | CTTGATTTGATTTTGAATAT+GGG | + | chr3_3:75255987-75256006 | Msa0472820:intron | 20.0% |
| !!! | TTGATTTGATTTTGAATATG+GGG | + | chr3_3:75255988-75256007 | Msa0472820:intron | 20.0% |
| !! | AAGTGAAATTCTATTCAATT+GGG | + | chr3_3:75256226-75256245 | Msa0472820:CDS | 20.0% |
| !!! | AACTAATTCTAAGAATGAAT+AGG | - | chr3_3:75256827-75256846 | None:intergenic | 20.0% |
| !! | TTGTTAATATTAGTTGAAGT+TGG | - | chr3_3:75257017-75257036 | None:intergenic | 20.0% |
| ! | CATTTGTAAAAAAAGAACGT+AGG | + | chr3_3:75255451-75255470 | Msa0472820:five_prime_UTR | 25.0% |
| !! | ATTGCTTAGGTTGTTATATA+AGG | - | chr3_3:75255609-75255628 | None:intergenic | 25.0% |
| ! | GAATTTGGAAGAATTTCAAA+AGG | - | chr3_3:75255962-75255981 | None:intergenic | 25.0% |
| !! | GGAAAATCAAGTTATTGATT+TGG | - | chr3_3:75256165-75256184 | None:intergenic | 25.0% |
| !! | TTGATTTTCCTCTTTTGATA+GGG | + | chr3_3:75256175-75256194 | Msa0472820:intron | 25.0% |
| ! | CAATTGAATAGAATTTCACT+TGG | - | chr3_3:75256227-75256246 | None:intergenic | 25.0% |
| ! | CAAGTGAAATTCTATTCAAT+TGG | + | chr3_3:75256225-75256244 | Msa0472820:CDS | 25.0% |
| ! | AGTGAAATTCTATTCAATTG+GGG | + | chr3_3:75256227-75256246 | Msa0472820:CDS | 25.0% |
| ! | AAAGAAGTTGGATTATAAAC+GGG | - | chr3_3:75256367-75256386 | None:intergenic | 25.0% |
| ! | GAAAGAAGTTGGATTATAAA+CGG | - | chr3_3:75256368-75256387 | None:intergenic | 25.0% |
| ! | AAGATATTATCGAAAGAAGT+TGG | - | chr3_3:75256379-75256398 | None:intergenic | 25.0% |
| ! | TGTTGTGATTAAAGAGATTA+AGG | + | chr3_3:75256579-75256598 | Msa0472820:CDS | 25.0% |
| ! | GTTGTGATTAAAGAGATTAA+GGG | + | chr3_3:75256580-75256599 | Msa0472820:CDS | 25.0% |
| ! | AGAATGAATAGGATCAATTA+AGG | - | chr3_3:75256816-75256835 | None:intergenic | 25.0% |
| ! | TATGAGATTTGAATTTCATC+CGG | + | chr3_3:75256944-75256963 | Msa0472820:intron | 25.0% |
| !!! | TGTTGCTATTTTGTACTAAT+TGG | + | chr3_3:75257288-75257307 | Msa0472820:three_prime_UTR | 25.0% |
| !!! | GTTGCTATTTTGTACTAATT+GGG | + | chr3_3:75257289-75257308 | Msa0472820:three_prime_UTR | 25.0% |
| AAAAAAGAACGTAGGAGTTT+AGG | + | chr3_3:75255459-75255478 | Msa0472820:five_prime_UTR | 30.0% | |
| AAGAATGGTAGATATTGCTT+AGG | - | chr3_3:75255622-75255641 | None:intergenic | 30.0% | |
| CTTAAGATGAGATGTAAGAA+TGG | - | chr3_3:75255637-75255656 | None:intergenic | 30.0% | |
| GTTTGTGAATTTGATTACAG+TGG | + | chr3_3:75255830-75255849 | Msa0472820:intron | 30.0% | |
| ! | CTTGATTTTCCTCTTTTGAT+AGG | + | chr3_3:75256174-75256193 | Msa0472820:intron | 30.0% |
| AGAATTTCACTTGGAGAAAA+TGG | - | chr3_3:75256218-75256237 | None:intergenic | 30.0% | |
| CTATTCAATTGGGGTTTATT+TGG | + | chr3_3:75256236-75256255 | Msa0472820:CDS | 30.0% | |
| GAGCTCGTCTATTTATTATT+TGG | + | chr3_3:75256434-75256453 | Msa0472820:intron | 30.0% | |
| TTCATCCTCATATTTGTCAT+CGG | - | chr3_3:75256656-75256675 | None:intergenic | 30.0% | |
| TGAATAGGATCAATTAAGGT+AGG | - | chr3_3:75256812-75256831 | None:intergenic | 30.0% | |
| !! | AGTTGATTTTAGGTACAAGT+TGG | + | chr3_3:75256849-75256868 | Msa0472820:intron | 30.0% |
| !! | TAAGAGTTTGATTTTGTCAC+CGG | - | chr3_3:75256966-75256985 | None:intergenic | 30.0% |
| ACAACAAACAATCATCACAT+TGG | - | chr3_3:75257058-75257077 | None:intergenic | 30.0% | |
| !! | GTTTGTTGTAATACAGATTG+TGG | + | chr3_3:75257069-75257088 | Msa0472820:intron | 30.0% |
| AATACAGATTGTGGTATCTT+TGG | + | chr3_3:75257078-75257097 | Msa0472820:intron | 30.0% | |
| TAGTGGAGACTATTAAGAAA+TGG | + | chr3_3:75257143-75257162 | Msa0472820:CDS | 30.0% | |
| ATTAAGAAATGGTATCTTGC+TGG | + | chr3_3:75257154-75257173 | Msa0472820:CDS | 30.0% | |
| ! | AACACAAAGTTTTCTTCACT+TGG | - | chr3_3:75257242-75257261 | None:intergenic | 30.0% |
| ACTTGTGACTTTCTTTCAAG+TGG | - | chr3_3:75255528-75255547 | None:intergenic | 35.0% | |
| CTACCACCTTAATTTAGCTA+CGG | + | chr3_3:75255565-75255584 | Msa0472820:five_prime_UTR | 35.0% | |
| TGGTAGTAGTGATCTTTGAA+GGG | - | chr3_3:75255890-75255909 | None:intergenic | 35.0% | |
| ! | TTGGTAGTAGTGATCTTTGA+AGG | - | chr3_3:75255891-75255910 | None:intergenic | 35.0% |
| CTAAAAGTAGCAAGAGATGT+AGG | + | chr3_3:75256105-75256124 | Msa0472820:intron | 35.0% | |
| ! | GTTTCTTGAAGTACTTAGAC+TGG | - | chr3_3:75256714-75256733 | None:intergenic | 35.0% |
| !!! | GATTTTAGGTACAAGTTGGT+TGG | + | chr3_3:75256853-75256872 | Msa0472820:intron | 35.0% |
| ! | AGATTGTGGTATCTTTGGAA+GGG | + | chr3_3:75257083-75257102 | Msa0472820:intron | 35.0% |
| AATAGAGAATGCCAATGTAG+TGG | + | chr3_3:75257126-75257145 | Msa0472820:CDS | 35.0% | |
| CTTAATAGTCTCCACTACAT+TGG | - | chr3_3:75257140-75257159 | None:intergenic | 35.0% | |
| TCCTTCAACCATTACATCTT+TGG | + | chr3_3:75257192-75257211 | Msa0472820:CDS | 35.0% | |
| CGTAGCTAAATTAAGGTGGT+AGG | - | chr3_3:75255567-75255586 | None:intergenic | 40.0% | |
| ACTCCGTAGCTAAATTAAGG+TGG | - | chr3_3:75255571-75255590 | None:intergenic | 40.0% | |
| CACACTCCGTAGCTAAATTA+AGG | - | chr3_3:75255574-75255593 | None:intergenic | 40.0% | |
| TAGAGAGAATTGTGGTGAGA+TGG | - | chr3_3:75255673-75255692 | None:intergenic | 40.0% | |
| AGAGGAAGTAGAGAGAATTG+TGG | - | chr3_3:75255681-75255700 | None:intergenic | 40.0% | |
| ATCGATGTAGATAGAGACAG+AGG | - | chr3_3:75255699-75255718 | None:intergenic | 40.0% | |
| AGCTCTTACTAGTTAGTTGC+TGG | - | chr3_3:75255724-75255743 | None:intergenic | 40.0% | |
| ! | AGTGGCTAGTGAAACTGTTT+TGG | + | chr3_3:75255848-75255867 | Msa0472820:intron | 40.0% |
| TCGGTAATCCCTATCAAAAG+AGG | - | chr3_3:75256186-75256205 | None:intergenic | 40.0% | |
| TTTGATAGGGATTACCGACA+TGG | + | chr3_3:75256188-75256207 | Msa0472820:intron | 40.0% | |
| GAGAAAATGGATCTCCATGT+CGG | - | chr3_3:75256205-75256224 | None:intergenic | 40.0% | |
| ATCATTGTCTTCCAACTCCT+TGG | - | chr3_3:75256307-75256326 | None:intergenic | 40.0% | |
| TTCTTTGACGCTCTCATTTC+TGG | + | chr3_3:75256330-75256349 | Msa0472820:CDS | 40.0% | |
| ! | GAGATTAAGGGTGCACAATA+TGG | + | chr3_3:75256592-75256611 | Msa0472820:CDS | 40.0% |
| GGATGAAGAAGAAGAAGCTT+TGG | + | chr3_3:75256669-75256688 | Msa0472820:CDS | 40.0% | |
| GTAAAGTAGCAAGCAGCATT+AGG | + | chr3_3:75256781-75256800 | Msa0472820:intron | 40.0% | |
| CAGATTGTGGTATCTTTGGA+AGG | + | chr3_3:75257082-75257101 | Msa0472820:intron | 40.0% | |
| GCCAAAGATGTAATGGTTGA+AGG | - | chr3_3:75257196-75257215 | None:intergenic | 40.0% | |
| ATGGGTAGCCAAAGATGTAA+TGG | - | chr3_3:75257203-75257222 | None:intergenic | 40.0% | |
| ! | GGATAATTCCTCTGAGAGAT+GGG | - | chr3_3:75257221-75257240 | None:intergenic | 40.0% |
| ! | TGGATAATTCCTCTGAGAGA+TGG | - | chr3_3:75257222-75257241 | None:intergenic | 40.0% |
| ! | GACTTTCTCCACTTTGTTTG+CGG | + | chr3_3:75257311-75257330 | Msa0472820:three_prime_UTR | 40.0% |
| ACCAAAACCCTTTCTCTCCT+TGG | + | chr3_3:75255906-75255925 | Msa0472820:CDS | 45.0% | |
| ! | ACCAAGGAGAGAAAGGGTTT+TGG | - | chr3_3:75255910-75255929 | None:intergenic | 45.0% |
| ACCATGACCAAGGAGAGAAA+GGG | - | chr3_3:75255916-75255935 | None:intergenic | 45.0% | |
| TACCATGACCAAGGAGAGAA+AGG | - | chr3_3:75255917-75255936 | None:intergenic | 45.0% | |
| GTAGCAACTTACCATGACCA+AGG | - | chr3_3:75255926-75255945 | None:intergenic | 45.0% | |
| TACCTTTCCACTGTTGCAAG+TGG | - | chr3_3:75256278-75256297 | None:intergenic | 45.0% | |
| AGTCTAATTGCCTTCTCCAC+AGG | - | chr3_3:75256559-75256578 | None:intergenic | 45.0% | |
| ! | ATTTGTCATCGGCTGCCAAA+CGG | - | chr3_3:75256645-75256664 | None:intergenic | 45.0% |
| !! | TGCTACTTTACCTCTGCAGT+TGG | - | chr3_3:75256772-75256791 | None:intergenic | 45.0% |
| !! | AATGGTATCTTGCTGGCTCA+AGG | + | chr3_3:75257161-75257180 | Msa0472820:CDS | 45.0% |
| !! | ATGGTATCTTGCTGGCTCAA+GGG | + | chr3_3:75257162-75257181 | Msa0472820:CDS | 45.0% |
| GCAGAATTCCGCAAACAAAG+TGG | - | chr3_3:75257322-75257341 | None:intergenic | 45.0% | |
| CATTGCACGTGTGGAATCTA+TGG | - | chr3_3:75257432-75257451 | None:intergenic | 45.0% | |
| CATAGATTCCACACGTGCAA+TGG | + | chr3_3:75257430-75257449 | Msa0472820:three_prime_UTR | 45.0% | |
| AAAGTTTGCCATTGCACGTG+TGG | - | chr3_3:75257441-75257460 | None:intergenic | 45.0% | |
| !! | TAAAAAAAAAAAAATTAAGT+TGG | - | chr3_3:75256912-75256931 | None:intergenic | 5.0% |
| ACCCTTTCTCTCCTTGGTCA+TGG | + | chr3_3:75255912-75255931 | Msa0472820:CDS | 50.0% | |
| !! | TGCAAGTGGCTTGCTACTTC+AGG | - | chr3_3:75256264-75256283 | None:intergenic | 50.0% |
| TAGCAAGCCACTTGCAACAG+TGG | + | chr3_3:75256268-75256287 | Msa0472820:CDS | 50.0% | |
| AGCCACTTGCAACAGTGGAA+AGG | + | chr3_3:75256273-75256292 | Msa0472820:CDS | 50.0% | |
| TTGTCTTCCAACTCCTTGGC+AGG | - | chr3_3:75256303-75256322 | None:intergenic | 50.0% | |
| GTGAATATGTGCAGCTCCTG+TGG | + | chr3_3:75256540-75256559 | Msa0472820:intron | 50.0% | |
| TTCAGATTGAGACCGCTGTG+AGG | + | chr3_3:75256617-75256636 | Msa0472820:CDS | 50.0% | |
| TCAGATTGAGACCGCTGTGA+GGG | + | chr3_3:75256618-75256637 | Msa0472820:CDS | 50.0% | |
| GGCAGCCGATGACAAATATG+AGG | + | chr3_3:75256648-75256667 | Msa0472820:CDS | 50.0% | |
| ! | CTGCAGTTGGAGAGTTTGCT+GGG | - | chr3_3:75256759-75256778 | None:intergenic | 50.0% |
| TCTGCAGTTGGAGAGTTTGC+TGG | - | chr3_3:75256760-75256779 | None:intergenic | 50.0% | |
| AGCAAACTCTCCAACTGCAG+AGG | + | chr3_3:75256759-75256778 | Msa0472820:CDS | 50.0% | |
| TTTGGCTACCCATCTCTCAG+AGG | + | chr3_3:75257210-75257229 | Msa0472820:CDS | 50.0% | |
| GTGGAAAGGTAAGCCTGCCA+AGG | + | chr3_3:75256287-75256306 | Msa0472820:CDS | 55.0% | |
| AGGTAAGCCTGCCAAGGAGT+TGG | + | chr3_3:75256293-75256312 | Msa0472820:CDS | 55.0% | |
| ATGTGCAGCTCCTGTGGAGA+AGG | + | chr3_3:75256546-75256565 | Msa0472820:intron | 55.0% | |
| TGCCAAACGGTCCCTCACAG+CGG | - | chr3_3:75256632-75256651 | None:intergenic | 60.0% | |
| GACCGCTGTGAGGGACCGTT+TGG | + | chr3_3:75256627-75256646 | Msa0472820:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3_3 | gene | 75255450 | 75257479 | 75255450 | ID=Msa0472820;Name=Msa0472820 |
| chr3_3 | mRNA | 75255450 | 75257479 | 75255450 | ID=Msa0472820-mRNA-1;Parent=Msa0472820;Name=Msa0472820-mRNA-1;_AED=0.03;_eAED=0.03;_QI=284|1|1|1|0.75|0.8|5|233|209 |
| chr3_3 | exon | 75255450 | 75255734 | 75255450 | ID=Msa0472820-mRNA-1:exon:19462;Parent=Msa0472820-mRNA-1 |
| chr3_3 | exon | 75255850 | 75255933 | 75255850 | ID=Msa0472820-mRNA-1:exon:19463;Parent=Msa0472820-mRNA-1 |
| chr3_3 | exon | 75256196 | 75256351 | 75256196 | ID=Msa0472820-mRNA-1:exon:19464;Parent=Msa0472820-mRNA-1 |
| chr3_3 | exon | 75256554 | 75256780 | 75256554 | ID=Msa0472820-mRNA-1:exon:19465;Parent=Msa0472820-mRNA-1 |
| chr3_3 | exon | 75257085 | 75257479 | 75257085 | ID=Msa0472820-mRNA-1:exon:19466;Parent=Msa0472820-mRNA-1 |
| chr3_3 | five_prime_UTR | 75255450 | 75255733 | 75255450 | ID=Msa0472820-mRNA-1:five_prime_utr;Parent=Msa0472820-mRNA-1 |
| chr3_3 | CDS | 75255734 | 75255734 | 75255734 | ID=Msa0472820-mRNA-1:cds;Parent=Msa0472820-mRNA-1 |
| chr3_3 | CDS | 75255850 | 75255933 | 75255850 | ID=Msa0472820-mRNA-1:cds;Parent=Msa0472820-mRNA-1 |
| chr3_3 | CDS | 75256196 | 75256351 | 75256196 | ID=Msa0472820-mRNA-1:cds;Parent=Msa0472820-mRNA-1 |
| chr3_3 | CDS | 75256554 | 75256780 | 75256554 | ID=Msa0472820-mRNA-1:cds;Parent=Msa0472820-mRNA-1 |
| chr3_3 | CDS | 75257085 | 75257246 | 75257085 | ID=Msa0472820-mRNA-1:cds;Parent=Msa0472820-mRNA-1 |
| chr3_3 | three_prime_UTR | 75257247 | 75257479 | 75257247 | ID=Msa0472820-mRNA-1:three_prime_utr;Parent=Msa0472820-mRNA-1 |
| Gene Sequence |
| Protein sequence |
