Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0298410 | XP_024632606.1 | 93.606 | 391 | 21 | 2 | 1 | 391 | 252 | 638 | 0.0 | 701 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0298410 | sp|Q66GQ2|Y5162_ARATH | 26.178 | 191 | 139 | 1 | 1 | 189 | 223 | 413 | 1.75e-13 | 75.5 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0298410 | G7IHH1 | 93.606 | 391 | 21 | 2 | 1 | 391 | 255 | 641 | 0.0 | 701 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0000050 | Msa0298410 | 0.811565 | 6.569337e-51 | -8.615850e-47 |
| Msa0001940 | Msa0298410 | 0.813221 | 2.855936e-51 | -8.615850e-47 |
| Msa0017670 | Msa0298410 | 0.800209 | 1.604339e-48 | -8.615850e-47 |
| Msa0017950 | Msa0298410 | 0.808572 | 2.899222e-50 | -8.615850e-47 |
| Msa0024550 | Msa0298410 | 0.814979 | 1.169309e-51 | -8.615850e-47 |
| Msa0027820 | Msa0298410 | 0.807536 | 4.817164e-50 | -8.615850e-47 |
| Msa0035120 | Msa0298410 | 0.831177 | 1.934675e-55 | -8.615850e-47 |
| Msa0072720 | Msa0298410 | 0.806554 | 7.776893e-50 | -8.615850e-47 |
| Msa0080580 | Msa0298410 | 0.825022 | 5.880574e-54 | -8.615850e-47 |
| Msa0089980 | Msa0298410 | 0.813338 | 2.691803e-51 | -8.615850e-47 |
| Msa0090010 | Msa0298410 | 0.815156 | 1.067877e-51 | -8.615850e-47 |
| Msa0093850 | Msa0298410 | 0.802210 | 6.249784e-49 | -8.615850e-47 |
| Msa0097770 | Msa0298410 | 0.801131 | 1.040637e-48 | -8.615850e-47 |
| Msa0098040 | Msa0298410 | 0.821830 | 3.276429e-53 | -8.615850e-47 |
| Msa0106620 | Msa0298410 | 0.818941 | 1.507406e-52 | -8.615850e-47 |
| Msa0106710 | Msa0298410 | 0.810800 | 9.627033e-51 | -8.615850e-47 |
| Msa0108410 | Msa0298410 | 0.830412 | 2.979847e-55 | -8.615850e-47 |
| Msa0120400 | Msa0298410 | 0.815055 | 1.124508e-51 | -8.615850e-47 |
| Msa0138360 | Msa0298410 | 0.804865 | 1.759556e-49 | -8.615850e-47 |
| Msa0186010 | Msa0298410 | 0.839530 | 1.499623e-57 | -8.615850e-47 |
| Msa0207350 | Msa0298410 | 0.817166 | 3.795721e-52 | -8.615850e-47 |
| Msa0214550 | Msa0298410 | 0.801897 | 7.247911e-49 | -8.615850e-47 |
| Msa0217700 | Msa0298410 | 0.809725 | 1.641954e-50 | -8.615850e-47 |
| Msa0219270 | Msa0298410 | 0.832269 | 1.040627e-55 | -8.615850e-47 |
| Msa0221000 | Msa0298410 | 0.809076 | 2.262596e-50 | -8.615850e-47 |
| Msa0225100 | Msa0298410 | 0.827641 | 1.398405e-54 | -8.615850e-47 |
| Msa0225480 | Msa0298410 | 0.805476 | 1.310874e-49 | -8.615850e-47 |
| Msa0234090 | Msa0298410 | 0.802541 | 5.342987e-49 | -8.615850e-47 |
| Msa0237210 | Msa0298410 | 0.800602 | 1.334340e-48 | -8.615850e-47 |
| Msa0240900 | Msa0298410 | 0.847497 | 1.114104e-59 | -8.615850e-47 |
| Msa0244640 | Msa0298410 | 0.814103 | 1.827156e-51 | -8.615850e-47 |
| Msa0251710 | Msa0298410 | 0.817350 | 3.451246e-52 | -8.615850e-47 |
| Msa0259210 | Msa0298410 | 0.822855 | 1.894169e-53 | -8.615850e-47 |
| Msa0259220 | Msa0298410 | 0.811232 | 7.761121e-51 | -8.615850e-47 |
| Msa0261170 | Msa0298410 | 0.804974 | 1.669806e-49 | -8.615850e-47 |
| Msa0265850 | Msa0298410 | 0.825548 | 4.414870e-54 | -8.615850e-47 |
| Msa0266180 | Msa0298410 | 0.832654 | 8.358093e-56 | -8.615850e-47 |
| Msa0276740 | Msa0298410 | 0.815573 | 8.626921e-52 | -8.615850e-47 |
| Msa0278570 | Msa0298410 | 0.807311 | 5.378494e-50 | -8.615850e-47 |
| Msa0279850 | Msa0298410 | 0.830518 | 2.806920e-55 | -8.615850e-47 |
| Msa0283330 | Msa0298410 | 0.818878 | 1.557847e-52 | -8.615850e-47 |
| Msa0287740 | Msa0298410 | 0.822292 | 2.560687e-53 | -8.615850e-47 |
| Msa0296820 | Msa0298410 | 0.806318 | 8.720715e-50 | -8.615850e-47 |
| Msa0298410 | Msa0300270 | 0.819940 | 8.920115e-53 | -8.615850e-47 |
| Msa0298410 | Msa0300320 | 0.835093 | 2.050113e-56 | -8.615850e-47 |
| Msa0298410 | Msa0307670 | 0.818859 | 1.573041e-52 | -8.615850e-47 |
| Msa0298410 | Msa0308630 | 0.800007 | 1.763974e-48 | -8.615850e-47 |
| Msa0298410 | Msa0316680 | 0.807242 | 5.563162e-50 | -8.615850e-47 |
| Msa0298410 | Msa0317970 | 0.851641 | 7.775237e-61 | -8.615850e-47 |
| Msa0298410 | Msa0320500 | 0.812506 | 4.096895e-51 | -8.615850e-47 |
| Msa0298410 | Msa0321620 | 0.814702 | 1.346705e-51 | -8.615850e-47 |
| Msa0298410 | Msa0322900 | 0.819163 | 1.341838e-52 | -8.615850e-47 |
| Msa0298410 | Msa0336110 | 0.836731 | 7.878460e-57 | -8.615850e-47 |
| Msa0298410 | Msa0336310 | 0.808211 | 3.462593e-50 | -8.615850e-47 |
| Msa0298410 | Msa0336320 | 0.815596 | 8.523298e-52 | -8.615850e-47 |
| Msa0298410 | Msa0337700 | 0.819382 | 1.195884e-52 | -8.615850e-47 |
| Msa0298410 | Msa0341690 | 0.815623 | 8.406246e-52 | -8.615850e-47 |
| Msa0298410 | Msa0378720 | 0.824391 | 8.279024e-54 | -8.615850e-47 |
| Msa0298410 | Msa0382370 | 0.810674 | 1.024954e-50 | -8.615850e-47 |
| Msa0298410 | Msa0403640 | 0.813944 | 1.980304e-51 | -8.615850e-47 |
| Msa0298410 | Msa0427100 | 0.802871 | 4.567399e-49 | -8.615850e-47 |
| Msa0298410 | Msa0427120 | 0.806205 | 9.211972e-50 | -8.615850e-47 |
| Msa0298410 | Msa0432160 | 0.827056 | 1.931670e-54 | -8.615850e-47 |
| Msa0298410 | Msa0432670 | 0.801078 | 1.066636e-48 | -8.615850e-47 |
| Msa0298410 | Msa0450400 | -0.829027 | 6.474506e-55 | -8.615850e-47 |
| Msa0298410 | Msa0460590 | 0.806956 | 6.395223e-50 | -8.615850e-47 |
| Msa0298410 | Msa0467100 | 0.803292 | 3.736616e-49 | -8.615850e-47 |
| Msa0298410 | Msa0474730 | 0.822475 | 2.321904e-53 | -8.615850e-47 |
| Msa0298410 | Msa0476680 | 0.829552 | 4.830234e-55 | -8.615850e-47 |
| Msa0298410 | Msa0479200 | 0.815236 | 1.025087e-51 | -8.615850e-47 |
| Msa0298410 | Msa0481970 | 0.802034 | 6.792380e-49 | -8.615850e-47 |
| Msa0298410 | Msa0485240 | 0.830164 | 3.425364e-55 | -8.615850e-47 |
| Msa0298410 | Msa0521010 | 0.828864 | 7.093815e-55 | -8.615850e-47 |
| Msa0298410 | Msa0527190 | 0.813219 | 2.860072e-51 | -8.615850e-47 |
| Msa0298410 | Msa0534400 | 0.808185 | 3.507385e-50 | -8.615850e-47 |
| Msa0298410 | Msa0580370 | 0.803116 | 4.063689e-49 | -8.615850e-47 |
| Msa0298410 | Msa0581470 | 0.808547 | 2.935235e-50 | -8.615850e-47 |
| Msa0298410 | Msa0583360 | -0.821583 | 3.737211e-53 | -8.615850e-47 |
| Msa0298410 | Msa0619050 | 0.804791 | 1.823022e-49 | -8.615850e-47 |
| Msa0298410 | Msa0624900 | 0.812825 | 3.489542e-51 | -8.615850e-47 |
| Msa0298410 | Msa0626460 | 0.809183 | 2.146532e-50 | -8.615850e-47 |
| Msa0298410 | Msa0630940 | 0.807519 | 4.858993e-50 | -8.615850e-47 |
| Msa0298410 | Msa0638480 | 0.812431 | 4.254351e-51 | -8.615850e-47 |
| Msa0298410 | Msa0659120 | 0.802229 | 6.195031e-49 | -8.615850e-47 |
| Msa0298410 | Msa0664540 | 0.818422 | 1.976353e-52 | -8.615850e-47 |
| Msa0298410 | Msa0693330 | 0.827125 | 1.859422e-54 | -8.615850e-47 |
| Msa0298410 | Msa0701360 | 0.812329 | 4.478087e-51 | -8.615850e-47 |
| Msa0298410 | Msa0702720 | 0.843563 | 1.297868e-58 | -8.615850e-47 |
| Msa0298410 | Msa0705580 | 0.821319 | 4.300997e-53 | -8.615850e-47 |
| Msa0298410 | Msa0715420 | 0.803710 | 3.061399e-49 | -8.615850e-47 |
| Msa0298410 | Msa0725000 | 0.801371 | 9.290889e-49 | -8.615850e-47 |
| Msa0298410 | Msa0752450 | 0.807339 | 5.306009e-50 | -8.615850e-47 |
| Msa0298410 | Msa0762910 | 0.845328 | 4.348555e-59 | -8.615850e-47 |
| Msa0298410 | Msa0769180 | 0.804263 | 2.348995e-49 | -8.615850e-47 |
| Msa0298410 | Msa0770330 | 0.821443 | 4.027850e-53 | -8.615850e-47 |
| Msa0298410 | Msa0792370 | 0.804298 | 2.310172e-49 | -8.615850e-47 |
| Msa0298410 | Msa0795870 | 0.814172 | 1.763857e-51 | -8.615850e-47 |
| Msa0298410 | Msa0805330 | 0.851472 | 8.682236e-61 | -8.615850e-47 |
| Msa0298410 | Msa0833530 | 0.816293 | 5.960422e-52 | -8.615850e-47 |
| Msa0298410 | Msa0833650 | 0.808678 | 2.752101e-50 | -8.615850e-47 |
| Msa0298410 | Msa0842680 | 0.834009 | 3.839022e-56 | -8.615850e-47 |
| Msa0298410 | Msa0855870 | 0.821718 | 3.479310e-53 | -8.615850e-47 |
| Msa0298410 | Msa0861510 | 0.805015 | 1.636942e-49 | -8.615850e-47 |
| Msa0298410 | Msa0871720 | 0.832502 | 9.115859e-56 | -8.615850e-47 |
| Msa0298410 | Msa0933760 | 0.817231 | 3.671455e-52 | -8.615850e-47 |
| Msa0298410 | Msa0934140 | 0.826024 | 3.403108e-54 | -8.615850e-47 |
| Msa0298410 | Msa0937020 | 0.812182 | 4.822059e-51 | -8.615850e-47 |
| Msa0298410 | Msa0967380 | 0.827805 | 1.276861e-54 | -8.615850e-47 |
| Msa0298410 | Msa0967870 | 0.827149 | 1.835016e-54 | -8.615850e-47 |
| Msa0298410 | Msa0971650 | 0.801063 | 1.074343e-48 | -8.615850e-47 |
| Msa0298410 | Msa1000920 | 0.833987 | 3.889529e-56 | -8.615850e-47 |
| Msa0298410 | Msa1034500 | 0.840577 | 7.996661e-58 | -8.615850e-47 |
| Msa0298410 | Msa1065880 | 0.805796 | 1.122914e-49 | -8.615850e-47 |
| Msa0298410 | Msa1074220 | 0.816513 | 5.319972e-52 | -8.615850e-47 |
| Msa0298410 | Msa1085330 | 0.802767 | 4.798688e-49 | -8.615850e-47 |
| Msa0298410 | Msa1087650 | 0.844550 | 7.052611e-59 | -8.615850e-47 |
| Msa0298410 | Msa1089450 | 0.850325 | 1.826506e-60 | -8.615850e-47 |
| Msa0298410 | Msa1111380 | 0.805333 | 1.404445e-49 | -8.615850e-47 |
| Msa0298410 | Msa1132840 | 0.805908 | 1.063809e-49 | -8.615850e-47 |
| Msa0298410 | Msa1145580 | 0.813257 | 2.805264e-51 | -8.615850e-47 |
| Msa0298410 | Msa1155170 | -0.808854 | 2.523622e-50 | -8.615850e-47 |
| Msa0298410 | Msa1162770 | 0.801543 | 8.570639e-49 | -8.615850e-47 |
| Msa0298410 | Msa1191180 | 0.801020 | 1.096504e-48 | -8.615850e-47 |
| Msa0298410 | Msa1204100 | -0.808175 | 3.523205e-50 | -8.615850e-47 |
| Msa0298410 | Msa1211230 | -0.822685 | 2.075523e-53 | -8.615850e-47 |
| Msa0298410 | Msa1211420 | -0.809105 | 2.230717e-50 | -8.615850e-47 |
| Msa0298410 | Msa1225860 | -0.823474 | 1.358612e-53 | -8.615850e-47 |
| Msa0298410 | Msa1226270 | 0.805901 | 1.067073e-49 | -8.615850e-47 |
| Msa0298410 | Msa1228200 | 0.810211 | 1.290568e-50 | -8.615850e-47 |
| Msa0298410 | Msa1243870 | 0.802666 | 5.035467e-49 | -8.615850e-47 |
| Msa0298410 | Msa1265590 | 0.821893 | 3.168515e-53 | -8.615850e-47 |
| Msa0298410 | Msa1267770 | 0.812175 | 4.839616e-51 | -8.615850e-47 |
| Msa0298410 | Msa1268240 | 0.807612 | 4.641548e-50 | -8.615850e-47 |
| Msa0298410 | Msa1277080 | 0.810453 | 1.143979e-50 | -8.615850e-47 |
| Msa0298410 | Msa1285550 | 0.823878 | 1.092393e-53 | -8.615850e-47 |
| Msa0298410 | Msa1299400 | -0.810605 | 1.061009e-50 | -8.615850e-47 |
| Msa0298410 | Msa1325920 | 0.804328 | 2.277632e-49 | -8.615850e-47 |
| Msa0298410 | Msa1329050 | -0.808493 | 3.014180e-50 | -8.615850e-47 |
| Msa0298410 | Msa1331050 | -0.814403 | 1.568604e-51 | -8.615850e-47 |
| Msa0298410 | Msa1335160 | 0.805289 | 1.434506e-49 | -8.615850e-47 |
| Msa0298410 | Msa1341840 | 0.831351 | 1.753464e-55 | -8.615850e-47 |
| Msa0298410 | Msa1348090 | 0.820568 | 6.402614e-53 | -8.615850e-47 |
| Msa0298410 | Msa1357790 | 0.827894 | 1.215822e-54 | -8.615850e-47 |
| Msa0298410 | Msa1369690 | 0.823281 | 1.507016e-53 | -8.615850e-47 |
| Msa0298410 | Msa1371520 | 0.803824 | 2.898609e-49 | -8.615850e-47 |
| Msa0298410 | Msa1390690 | 0.800991 | 1.111709e-48 | -8.615850e-47 |
| Msa0298410 | Msa1390700 | 0.816581 | 5.135641e-52 | -8.615850e-47 |
| Msa0298410 | Msa1392910 | 0.810515 | 1.109537e-50 | -8.615850e-47 |
| Msa0298410 | Msa1393820 | 0.802601 | 5.191230e-49 | -8.615850e-47 |
| Msa0298410 | Msa1403710 | 0.803426 | 3.505185e-49 | -8.615850e-47 |
| Msa0298410 | Msa1418240 | 0.829649 | 4.574898e-55 | -8.615850e-47 |
| Msa0298410 | Msa1423780 | 0.808705 | 2.716159e-50 | -8.615850e-47 |
| Msa0298410 | Msa1446330 | 0.800165 | 1.637934e-48 | -8.615850e-47 |
| Msa0298410 | Msa1449860 | 0.801800 | 7.589000e-49 | -8.615850e-47 |
| Msa0298410 | Msa1461370 | 0.823570 | 1.289922e-53 | -8.615850e-47 |
| Msa0298410 | Msa1461540 | 0.818325 | 2.078617e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0298410 | MtrunA17_Chr2g0321731 | 93.606 | 391 | 21 | 2 | 1 | 391 | 255 | 641 | 0.0 | 701 |
| Msa0298410 | MtrunA17_Chr4g0026051 | 51.134 | 397 | 171 | 10 | 1 | 391 | 248 | 627 | 6.25e-101 | 312 |
| Msa0298410 | MtrunA17_Chr7g0266861 | 41.689 | 379 | 165 | 13 | 1 | 349 | 252 | 604 | 1.97e-69 | 231 |
| Msa0298410 | MtrunA17_Chr3g0098801 | 30.060 | 336 | 177 | 6 | 4 | 300 | 230 | 546 | 8.74e-33 | 130 |
| Msa0298410 | MtrunA17_Chr5g0444041 | 41.530 | 183 | 107 | 0 | 4 | 186 | 219 | 401 | 2.50e-28 | 117 |
| Msa0298410 | MtrunA17_Chr2g0288331 | 36.022 | 186 | 119 | 0 | 4 | 189 | 172 | 357 | 1.74e-22 | 99.0 |
| Msa0298410 | MtrunA17_Chr5g0397031 | 28.272 | 191 | 136 | 1 | 1 | 190 | 226 | 416 | 2.84e-22 | 99.0 |
| Msa0298410 | MtrunA17_Chr7g0275251 | 29.167 | 216 | 138 | 5 | 4 | 210 | 165 | 374 | 1.46e-17 | 84.3 |
| Msa0298410 | MtrunA17_Chr8g0347871 | 28.877 | 187 | 132 | 1 | 1 | 186 | 194 | 380 | 2.64e-17 | 83.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0298410 | AT3G11590.2 | 49.811 | 265 | 121 | 6 | 1 | 260 | 98 | 355 | 1.16e-56 | 192 |
| Msa0298410 | AT3G11590.1 | 49.811 | 265 | 121 | 6 | 1 | 260 | 260 | 517 | 4.22e-56 | 194 |
| Msa0298410 | AT1G50660.1 | 39.130 | 184 | 112 | 0 | 4 | 187 | 245 | 428 | 1.43e-34 | 135 |
| Msa0298410 | AT5G22310.1 | 39.409 | 203 | 82 | 5 | 54 | 256 | 248 | 409 | 1.62e-30 | 122 |
| Msa0298410 | AT3G20350.1 | 34.884 | 215 | 130 | 2 | 8 | 222 | 226 | 430 | 2.41e-30 | 123 |
| Msa0298410 | AT5G41620.4 | 26.178 | 191 | 139 | 1 | 1 | 189 | 106 | 296 | 1.58e-14 | 75.5 |
| Msa0298410 | AT5G41620.3 | 26.178 | 191 | 139 | 1 | 1 | 189 | 106 | 296 | 1.58e-14 | 75.5 |
| Msa0298410 | AT5G41620.2 | 26.178 | 191 | 139 | 1 | 1 | 189 | 64 | 254 | 1.75e-14 | 75.1 |
| Msa0298410 | AT5G41620.1 | 26.178 | 191 | 139 | 1 | 1 | 189 | 223 | 413 | 1.78e-14 | 75.5 |
Find 52 sgRNAs with CRISPR-Local
Find 136 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATTATCTTCCTCTAGATTT+TGG | 0.099398 | 2_3:+52374395 | None:intergenic |
| GAAAAGGGCTAAAGAGATTT+TGG | 0.212294 | 2_3:-52374755 | Msa0298410:CDS |
| TCAATCTATTGAATTGAATA+TGG | 0.239027 | 2_3:-52374332 | Msa0298410:CDS |
| CCACTTTCTTTATCAAAATC+TGG | 0.249092 | 2_3:+52373992 | None:intergenic |
| GTTTCAATTGATAAAGATAT+AGG | 0.289690 | 2_3:-52374241 | Msa0298410:CDS |
| TAGACTCATAATAGGCTTAT+AGG | 0.291883 | 2_3:+52374033 | None:intergenic |
| GAATTGATCGATTTGGTTGC+GGG | 0.324724 | 2_3:+52375023 | None:intergenic |
| TTTCAATTGATAAAGATATA+GGG | 0.337013 | 2_3:-52374240 | Msa0298410:CDS |
| CATGAAGATTTCCAAAGAAA+TGG | 0.357190 | 2_3:-52374827 | Msa0298410:CDS |
| ATACTCAATATTGTTCTGAT+TGG | 0.361199 | 2_3:+52374984 | None:intergenic |
| TGGTCTCTTAGACTCATAAT+AGG | 0.362914 | 2_3:+52374025 | None:intergenic |
| CTTGAATGAATTGATCGATT+TGG | 0.380451 | 2_3:+52375016 | None:intergenic |
| CAAAATCTGGAAATGATATA+TGG | 0.386837 | 2_3:+52374005 | None:intergenic |
| AATTCGAGAAGAAGTTGAAA+AGG | 0.392229 | 2_3:-52374653 | Msa0298410:CDS |
| AGTATCAATTTGAAGAGAAA+AGG | 0.393878 | 2_3:-52374561 | Msa0298410:CDS |
| AGAGGAAAATGCCCTGGCTT+TGG | 0.424601 | 2_3:-52373933 | Msa0298410:CDS |
| TCTATCTTCTCCAACACCTT+TGG | 0.426390 | 2_3:+52374711 | None:intergenic |
| TGGCTTCTGAGAGCTTCATA+TGG | 0.428417 | 2_3:+52374584 | None:intergenic |
| TTTCTCTTCAAATTGATACT+TGG | 0.434157 | 2_3:+52374564 | None:intergenic |
| GGAAAATGCCCTGGCTTTGG+AGG | 0.439312 | 2_3:-52373930 | Msa0298410:CDS |
| TGAGTTTGAGGTACATTGAT+GGG | 0.446281 | 2_3:-52373961 | Msa0298410:CDS |
| CACAAGTAGAGGAAATGAAA+AGG | 0.450688 | 2_3:-52374687 | Msa0298410:CDS |
| ATTGTCGAAAGAACACGAAA+TGG | 0.459265 | 2_3:-52374779 | Msa0298410:CDS |
| AGAACACGAAATGGAGAAAA+GGG | 0.459626 | 2_3:-52374770 | Msa0298410:CDS |
| AAGAACACGAAATGGAGAAA+AGG | 0.461476 | 2_3:-52374771 | Msa0298410:CDS |
| TATCATGTCCCTCCAAAGCC+AGG | 0.466896 | 2_3:+52373921 | None:intergenic |
| TTGAAGAGGAAAAGATTGCT+TGG | 0.472224 | 2_3:-52374948 | Msa0298410:CDS |
| AGAAATAAAGAAGAAGAGAA+AGG | 0.475340 | 2_3:-52374496 | Msa0298410:CDS |
| AGAGGAAATGAAAAGGGAAT+CGG | 0.478419 | 2_3:-52374680 | Msa0298410:CDS |
| GATGATATTGATGTTAATCC+AGG | 0.478815 | 2_3:-52374472 | Msa0298410:CDS |
| TTGAATTTGAGAAGAAGCTA+AGG | 0.484393 | 2_3:-52374870 | Msa0298410:CDS |
| TTGAGTTTGAGGTACATTGA+TGG | 0.490634 | 2_3:-52373962 | Msa0298410:CDS |
| GTATCAATTTGAAGAGAAAA+GGG | 0.497216 | 2_3:-52374560 | Msa0298410:CDS |
| TCCTTGAGTTTCTTAAGACC+TGG | 0.504987 | 2_3:+52374454 | None:intergenic |
| TGAATTGATCGATTTGGTTG+CGG | 0.523191 | 2_3:+52375022 | None:intergenic |
| ACAAGTAGAGGAAATGAAAA+GGG | 0.531779 | 2_3:-52374686 | Msa0298410:CDS |
| AGATGATAAAGAAGAAAATG+AGG | 0.536416 | 2_3:-52374062 | Msa0298410:CDS |
| TTTGGAATTCCAAAATCTAG+AGG | 0.564517 | 2_3:-52374404 | Msa0298410:CDS |
| GGGAAGTATTTGCTTCAACA+AGG | 0.569289 | 2_3:-52374197 | Msa0298410:CDS |
| GGAAGTATTTGCTTCAACAA+GGG | 0.586753 | 2_3:-52374196 | Msa0298410:CDS |
| GATGATAAAGAAGAAAATGA+GGG | 0.588141 | 2_3:-52374061 | Msa0298410:CDS |
| GATGAATTAGCCAAAGGTGT+TGG | 0.590367 | 2_3:-52374721 | Msa0298410:CDS |
| AGAAGATAGATCACAAGTAG+AGG | 0.595577 | 2_3:-52374698 | Msa0298410:CDS |
| GAGTTTGAGGTACATTGATG+GGG | 0.599467 | 2_3:-52373960 | Msa0298410:CDS |
| GAAAATGCCCTGGCTTTGGA+GGG | 0.600285 | 2_3:-52373929 | Msa0298410:CDS |
| GGAAGCAGAGGAAAATGCCC+TGG | 0.603191 | 2_3:-52373939 | Msa0298410:CDS |
| TCCAGGTCTTAAGAAACTCA+AGG | 0.612971 | 2_3:-52374455 | Msa0298410:CDS |
| GTACATTGATGGGGAAGCAG+AGG | 0.621003 | 2_3:-52373951 | Msa0298410:CDS |
| AATTTGAGAAGAAGCTAAGG+AGG | 0.621879 | 2_3:-52374867 | Msa0298410:CDS |
| ATCATGTCCCTCCAAAGCCA+GGG | 0.630793 | 2_3:+52373922 | None:intergenic |
| GTTTGTGATGAATTAGCCAA+AGG | 0.638876 | 2_3:-52374727 | Msa0298410:CDS |
| AGAAAGAGAGAAGATTCGCG+AGG | 0.695830 | 2_3:-52374917 | Msa0298410:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATCAATTGAATTTATTTT+TGG | - | chr2_3:52374840-52374859 | Msa0298410:CDS | 10.0% |
| !!! | AAATCAATTGAATTTATTTT+TGG | - | chr2_3:52374840-52374859 | Msa0298410:CDS | 10.0% |
| !! | TTTCAATTGATAAAGATATA+GGG | - | chr2_3:52374697-52374716 | Msa0298410:CDS | 15.0% |
| !! | TTTCAATTGATAAAGATATA+GGG | - | chr2_3:52374697-52374716 | Msa0298410:CDS | 15.0% |
| !!! | CTATTTGAACAAAACATTTT+TGG | - | chr2_3:52374515-52374534 | Msa0298410:CDS | 20.0% |
| !! | TCAATCTATTGAATTGAATA+TGG | - | chr2_3:52374605-52374624 | Msa0298410:CDS | 20.0% |
| !! | GTTTCAATTGATAAAGATAT+AGG | - | chr2_3:52374696-52374715 | Msa0298410:CDS | 20.0% |
| !! | TACAAATAGTACTAAAAAGA+AGG | - | chr2_3:52374767-52374786 | Msa0298410:CDS | 20.0% |
| !!! | CTATTTGAACAAAACATTTT+TGG | - | chr2_3:52374515-52374534 | Msa0298410:CDS | 20.0% |
| !! | TCAATCTATTGAATTGAATA+TGG | - | chr2_3:52374605-52374624 | Msa0298410:CDS | 20.0% |
| !! | GTTTCAATTGATAAAGATAT+AGG | - | chr2_3:52374696-52374715 | Msa0298410:CDS | 20.0% |
| !! | TACAAATAGTACTAAAAAGA+AGG | - | chr2_3:52374767-52374786 | Msa0298410:CDS | 20.0% |
| ! | ATACTCAATATTGTTCTGAT+TGG | + | chr2_3:52373956-52373975 | None:intergenic | 25.0% |
| !! | TTTTATGTTCTCCATTTCTT+TGG | + | chr2_3:52374124-52374143 | None:intergenic | 25.0% |
| !! | TTCTTTCGACAATTTTGAAT+AGG | + | chr2_3:52374151-52374170 | None:intergenic | 25.0% |
| ! | TTTCTCTTCAAATTGATACT+TGG | + | chr2_3:52374376-52374395 | None:intergenic | 25.0% |
| ! | AGTATCAATTTGAAGAGAAA+AGG | - | chr2_3:52374376-52374395 | Msa0298410:CDS | 25.0% |
| ! | GTATCAATTTGAAGAGAAAA+GGG | - | chr2_3:52374377-52374396 | Msa0298410:CDS | 25.0% |
| ! | AGAAATAAAGAAGAAGAGAA+AGG | - | chr2_3:52374441-52374460 | Msa0298410:CDS | 25.0% |
| ! | ATGACAAAAAAAGTTACAAG+TGG | - | chr2_3:52374631-52374650 | Msa0298410:CDS | 25.0% |
| !!! | GAGAAGATCTTTTAAAGATT+GGG | - | chr2_3:52374719-52374738 | Msa0298410:CDS | 25.0% |
| !!! | AGAAGATCTTTTAAAGATTG+GGG | - | chr2_3:52374720-52374739 | Msa0298410:CDS | 25.0% |
| ! | AGATGATAAAGAAGAAAATG+AGG | - | chr2_3:52374875-52374894 | Msa0298410:CDS | 25.0% |
| ! | GATGATAAAGAAGAAAATGA+GGG | - | chr2_3:52374876-52374895 | Msa0298410:CDS | 25.0% |
| ! | CAAAATCTGGAAATGATATA+TGG | + | chr2_3:52374935-52374954 | None:intergenic | 25.0% |
| ! | ATACTCAATATTGTTCTGAT+TGG | + | chr2_3:52373956-52373975 | None:intergenic | 25.0% |
| !! | TTTTATGTTCTCCATTTCTT+TGG | + | chr2_3:52374124-52374143 | None:intergenic | 25.0% |
| !! | TTCTTTCGACAATTTTGAAT+AGG | + | chr2_3:52374151-52374170 | None:intergenic | 25.0% |
| ! | TTTCTCTTCAAATTGATACT+TGG | + | chr2_3:52374376-52374395 | None:intergenic | 25.0% |
| ! | AGTATCAATTTGAAGAGAAA+AGG | - | chr2_3:52374376-52374395 | Msa0298410:CDS | 25.0% |
| ! | GTATCAATTTGAAGAGAAAA+GGG | - | chr2_3:52374377-52374396 | Msa0298410:CDS | 25.0% |
| ! | AGAAATAAAGAAGAAGAGAA+AGG | - | chr2_3:52374441-52374460 | Msa0298410:CDS | 25.0% |
| ! | ATGACAAAAAAAGTTACAAG+TGG | - | chr2_3:52374631-52374650 | Msa0298410:CDS | 25.0% |
| !!! | GAGAAGATCTTTTAAAGATT+GGG | - | chr2_3:52374719-52374738 | Msa0298410:CDS | 25.0% |
| !!! | AGAAGATCTTTTAAAGATTG+GGG | - | chr2_3:52374720-52374739 | Msa0298410:CDS | 25.0% |
| ! | AGATGATAAAGAAGAAAATG+AGG | - | chr2_3:52374875-52374894 | Msa0298410:CDS | 25.0% |
| ! | GATGATAAAGAAGAAAATGA+GGG | - | chr2_3:52374876-52374895 | Msa0298410:CDS | 25.0% |
| ! | CAAAATCTGGAAATGATATA+TGG | + | chr2_3:52374935-52374954 | None:intergenic | 25.0% |
| CTTGAATGAATTGATCGATT+TGG | + | chr2_3:52373924-52373943 | None:intergenic | 30.0% | |
| TTGAATTTGAGAAGAAGCTA+AGG | - | chr2_3:52374067-52374086 | Msa0298410:CDS | 30.0% | |
| CATGAAGATTTCCAAAGAAA+TGG | - | chr2_3:52374110-52374129 | Msa0298410:CDS | 30.0% | |
| ACAAGTAGAGGAAATGAAAA+GGG | - | chr2_3:52374251-52374270 | Msa0298410:CDS | 30.0% | |
| AATTCGAGAAGAAGTTGAAA+AGG | - | chr2_3:52374284-52374303 | Msa0298410:CDS | 30.0% | |
| GATGATATTGATGTTAATCC+AGG | - | chr2_3:52374465-52374484 | Msa0298410:CDS | 30.0% | |
| ! | TTTGGAATTCCAAAATCTAG+AGG | - | chr2_3:52374533-52374552 | Msa0298410:CDS | 30.0% |
| ! | CATTATCTTCCTCTAGATTT+TGG | + | chr2_3:52374545-52374564 | None:intergenic | 30.0% |
| !!! | TGAGAATATTCCACTTTTTG+AGG | - | chr2_3:52374665-52374684 | Msa0298410:CDS | 30.0% |
| !! | GGAGAAGATCTTTTAAAGAT+TGG | - | chr2_3:52374718-52374737 | Msa0298410:CDS | 30.0% |
| ACTAAAAAGAAGGACTTTGA+TGG | - | chr2_3:52374777-52374796 | Msa0298410:CDS | 30.0% | |
| TAGACTCATAATAGGCTTAT+AGG | + | chr2_3:52374907-52374926 | None:intergenic | 30.0% | |
| CCACTTTCTTTATCAAAATC+TGG | + | chr2_3:52374948-52374967 | None:intergenic | 30.0% | |
| !! | CCAGATTTTGATAAAGAAAG+TGG | - | chr2_3:52374945-52374964 | Msa0298410:CDS | 30.0% |
| CTTGAATGAATTGATCGATT+TGG | + | chr2_3:52373924-52373943 | None:intergenic | 30.0% | |
| TTGAATTTGAGAAGAAGCTA+AGG | - | chr2_3:52374067-52374086 | Msa0298410:CDS | 30.0% | |
| CATGAAGATTTCCAAAGAAA+TGG | - | chr2_3:52374110-52374129 | Msa0298410:CDS | 30.0% | |
| ACAAGTAGAGGAAATGAAAA+GGG | - | chr2_3:52374251-52374270 | Msa0298410:CDS | 30.0% | |
| AATTCGAGAAGAAGTTGAAA+AGG | - | chr2_3:52374284-52374303 | Msa0298410:CDS | 30.0% | |
| GATGATATTGATGTTAATCC+AGG | - | chr2_3:52374465-52374484 | Msa0298410:CDS | 30.0% | |
| ! | TTTGGAATTCCAAAATCTAG+AGG | - | chr2_3:52374533-52374552 | Msa0298410:CDS | 30.0% |
| ! | CATTATCTTCCTCTAGATTT+TGG | + | chr2_3:52374545-52374564 | None:intergenic | 30.0% |
| !!! | TGAGAATATTCCACTTTTTG+AGG | - | chr2_3:52374665-52374684 | Msa0298410:CDS | 30.0% |
| !! | GGAGAAGATCTTTTAAAGAT+TGG | - | chr2_3:52374718-52374737 | Msa0298410:CDS | 30.0% |
| ACTAAAAAGAAGGACTTTGA+TGG | - | chr2_3:52374777-52374796 | Msa0298410:CDS | 30.0% | |
| TAGACTCATAATAGGCTTAT+AGG | + | chr2_3:52374907-52374926 | None:intergenic | 30.0% | |
| CCACTTTCTTTATCAAAATC+TGG | + | chr2_3:52374948-52374967 | None:intergenic | 30.0% | |
| !! | CCAGATTTTGATAAAGAAAG+TGG | - | chr2_3:52374945-52374964 | Msa0298410:CDS | 30.0% |
| !! | TGAATTGATCGATTTGGTTG+CGG | + | chr2_3:52373918-52373937 | None:intergenic | 35.0% |
| !! | TCTCATGAAGCATTTTGAAG+AGG | - | chr2_3:52373975-52373994 | Msa0298410:CDS | 35.0% |
| TTGAAGAGGAAAAGATTGCT+TGG | - | chr2_3:52373989-52374008 | Msa0298410:CDS | 35.0% | |
| AATTTGAGAAGAAGCTAAGG+AGG | - | chr2_3:52374070-52374089 | Msa0298410:CDS | 35.0% | |
| ATTGTCGAAAGAACACGAAA+TGG | - | chr2_3:52374158-52374177 | Msa0298410:CDS | 35.0% | |
| AAGAACACGAAATGGAGAAA+AGG | - | chr2_3:52374166-52374185 | Msa0298410:CDS | 35.0% | |
| AGAACACGAAATGGAGAAAA+GGG | - | chr2_3:52374167-52374186 | Msa0298410:CDS | 35.0% | |
| ! | GAAAAGGGCTAAAGAGATTT+TGG | - | chr2_3:52374182-52374201 | Msa0298410:CDS | 35.0% |
| GTTTGTGATGAATTAGCCAA+AGG | - | chr2_3:52374210-52374229 | Msa0298410:CDS | 35.0% | |
| AGAAGATAGATCACAAGTAG+AGG | - | chr2_3:52374239-52374258 | Msa0298410:CDS | 35.0% | |
| CACAAGTAGAGGAAATGAAA+AGG | - | chr2_3:52374250-52374269 | Msa0298410:CDS | 35.0% | |
| AGAGGAAATGAAAAGGGAAT+CGG | - | chr2_3:52374257-52374276 | Msa0298410:CDS | 35.0% | |
| ! | ACTCTTTTTGCCTCAAAAAG+TGG | + | chr2_3:52374678-52374697 | None:intergenic | 35.0% |
| !! | GGAAGTATTTGCTTCAACAA+GGG | - | chr2_3:52374741-52374760 | Msa0298410:CDS | 35.0% |
| !! | AGTTTTGATCAGCTTGAAAG+TGG | - | chr2_3:52374816-52374835 | Msa0298410:CDS | 35.0% |
| TGGTCTCTTAGACTCATAAT+AGG | + | chr2_3:52374915-52374934 | None:intergenic | 35.0% | |
| !!! | GTGGTAAACTTTTGAGTTTG+AGG | - | chr2_3:52374964-52374983 | Msa0298410:CDS | 35.0% |
| ! | TTGAGTTTGAGGTACATTGA+TGG | - | chr2_3:52374975-52374994 | Msa0298410:CDS | 35.0% |
| ! | TGAGTTTGAGGTACATTGAT+GGG | - | chr2_3:52374976-52374995 | Msa0298410:CDS | 35.0% |
| !! | TGAATTGATCGATTTGGTTG+CGG | + | chr2_3:52373918-52373937 | None:intergenic | 35.0% |
| !! | TCTCATGAAGCATTTTGAAG+AGG | - | chr2_3:52373975-52373994 | Msa0298410:CDS | 35.0% |
| TTGAAGAGGAAAAGATTGCT+TGG | - | chr2_3:52373989-52374008 | Msa0298410:CDS | 35.0% | |
| AATTTGAGAAGAAGCTAAGG+AGG | - | chr2_3:52374070-52374089 | Msa0298410:CDS | 35.0% | |
| ATTGTCGAAAGAACACGAAA+TGG | - | chr2_3:52374158-52374177 | Msa0298410:CDS | 35.0% | |
| AAGAACACGAAATGGAGAAA+AGG | - | chr2_3:52374166-52374185 | Msa0298410:CDS | 35.0% | |
| AGAACACGAAATGGAGAAAA+GGG | - | chr2_3:52374167-52374186 | Msa0298410:CDS | 35.0% | |
| ! | GAAAAGGGCTAAAGAGATTT+TGG | - | chr2_3:52374182-52374201 | Msa0298410:CDS | 35.0% |
| GTTTGTGATGAATTAGCCAA+AGG | - | chr2_3:52374210-52374229 | Msa0298410:CDS | 35.0% | |
| AGAAGATAGATCACAAGTAG+AGG | - | chr2_3:52374239-52374258 | Msa0298410:CDS | 35.0% | |
| CACAAGTAGAGGAAATGAAA+AGG | - | chr2_3:52374250-52374269 | Msa0298410:CDS | 35.0% | |
| AGAGGAAATGAAAAGGGAAT+CGG | - | chr2_3:52374257-52374276 | Msa0298410:CDS | 35.0% | |
| ! | ACTCTTTTTGCCTCAAAAAG+TGG | + | chr2_3:52374678-52374697 | None:intergenic | 35.0% |
| !! | GGAAGTATTTGCTTCAACAA+GGG | - | chr2_3:52374741-52374760 | Msa0298410:CDS | 35.0% |
| !! | AGTTTTGATCAGCTTGAAAG+TGG | - | chr2_3:52374816-52374835 | Msa0298410:CDS | 35.0% |
| TGGTCTCTTAGACTCATAAT+AGG | + | chr2_3:52374915-52374934 | None:intergenic | 35.0% | |
| !!! | GTGGTAAACTTTTGAGTTTG+AGG | - | chr2_3:52374964-52374983 | Msa0298410:CDS | 35.0% |
| ! | TTGAGTTTGAGGTACATTGA+TGG | - | chr2_3:52374975-52374994 | Msa0298410:CDS | 35.0% |
| ! | TGAGTTTGAGGTACATTGAT+GGG | - | chr2_3:52374976-52374995 | Msa0298410:CDS | 35.0% |
| !! | GAATTGATCGATTTGGTTGC+GGG | + | chr2_3:52373917-52373936 | None:intergenic | 40.0% |
| ! | GATGAATTAGCCAAAGGTGT+TGG | - | chr2_3:52374216-52374235 | Msa0298410:CDS | 40.0% |
| TCTATCTTCTCCAACACCTT+TGG | + | chr2_3:52374229-52374248 | None:intergenic | 40.0% | |
| TCCAGGTCTTAAGAAACTCA+AGG | - | chr2_3:52374482-52374501 | Msa0298410:CDS | 40.0% | |
| TCCTTGAGTTTCTTAAGACC+TGG | + | chr2_3:52374486-52374505 | None:intergenic | 40.0% | |
| !! | GGGAAGTATTTGCTTCAACA+AGG | - | chr2_3:52374740-52374759 | Msa0298410:CDS | 40.0% |
| ! | GAGTTTGAGGTACATTGATG+GGG | - | chr2_3:52374977-52374996 | Msa0298410:CDS | 40.0% |
| !! | GAATTGATCGATTTGGTTGC+GGG | + | chr2_3:52373917-52373936 | None:intergenic | 40.0% |
| ! | GATGAATTAGCCAAAGGTGT+TGG | - | chr2_3:52374216-52374235 | Msa0298410:CDS | 40.0% |
| TCTATCTTCTCCAACACCTT+TGG | + | chr2_3:52374229-52374248 | None:intergenic | 40.0% | |
| TCCAGGTCTTAAGAAACTCA+AGG | - | chr2_3:52374482-52374501 | Msa0298410:CDS | 40.0% | |
| TCCTTGAGTTTCTTAAGACC+TGG | + | chr2_3:52374486-52374505 | None:intergenic | 40.0% | |
| !! | GGGAAGTATTTGCTTCAACA+AGG | - | chr2_3:52374740-52374759 | Msa0298410:CDS | 40.0% |
| ! | GAGTTTGAGGTACATTGATG+GGG | - | chr2_3:52374977-52374996 | Msa0298410:CDS | 40.0% |
| AGAAAGAGAGAAGATTCGCG+AGG | - | chr2_3:52374020-52374039 | Msa0298410:CDS | 45.0% | |
| ! | TGGCTTCTGAGAGCTTCATA+TGG | + | chr2_3:52374356-52374375 | None:intergenic | 45.0% |
| AGAAAGAGAGAAGATTCGCG+AGG | - | chr2_3:52374020-52374039 | Msa0298410:CDS | 45.0% | |
| ! | TGGCTTCTGAGAGCTTCATA+TGG | + | chr2_3:52374356-52374375 | None:intergenic | 45.0% |
| GTACATTGATGGGGAAGCAG+AGG | - | chr2_3:52374986-52375005 | Msa0298410:CDS | 50.0% | |
| AGAGGAAAATGCCCTGGCTT+TGG | - | chr2_3:52375004-52375023 | Msa0298410:CDS | 50.0% | |
| !! | GAAAATGCCCTGGCTTTGGA+GGG | - | chr2_3:52375008-52375027 | Msa0298410:CDS | 50.0% |
| ATCATGTCCCTCCAAAGCCA+GGG | + | chr2_3:52375018-52375037 | None:intergenic | 50.0% | |
| TATCATGTCCCTCCAAAGCC+AGG | + | chr2_3:52375019-52375038 | None:intergenic | 50.0% | |
| GTACATTGATGGGGAAGCAG+AGG | - | chr2_3:52374986-52375005 | Msa0298410:CDS | 50.0% | |
| AGAGGAAAATGCCCTGGCTT+TGG | - | chr2_3:52375004-52375023 | Msa0298410:CDS | 50.0% | |
| !! | GAAAATGCCCTGGCTTTGGA+GGG | - | chr2_3:52375008-52375027 | Msa0298410:CDS | 50.0% |
| ATCATGTCCCTCCAAAGCCA+GGG | + | chr2_3:52375018-52375037 | None:intergenic | 50.0% | |
| TATCATGTCCCTCCAAAGCC+AGG | + | chr2_3:52375019-52375038 | None:intergenic | 50.0% | |
| GGAAGCAGAGGAAAATGCCC+TGG | - | chr2_3:52374998-52375017 | Msa0298410:CDS | 55.0% | |
| !! | GGAAAATGCCCTGGCTTTGG+AGG | - | chr2_3:52375007-52375026 | Msa0298410:CDS | 55.0% |
| GGAAGCAGAGGAAAATGCCC+TGG | - | chr2_3:52374998-52375017 | Msa0298410:CDS | 55.0% | |
| !! | GGAAAATGCCCTGGCTTTGG+AGG | - | chr2_3:52375007-52375026 | Msa0298410:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_3 | gene | 52373892 | 52375067 | 52373892 | ID=Msa0298410;Name=Msa0298410 |
| chr2_3 | mRNA | 52373892 | 52375067 | 52373892 | ID=Msa0298410-mRNA-1;Parent=Msa0298410;Name=Msa0298410-mRNA-1;_AED=0.35;_eAED=0.35;_QI=0|-1|0|1|-1|1|1|0|391 |
| chr2_3 | exon | 52373892 | 52375067 | 52373892 | ID=Msa0298410-mRNA-1:exon:16729;Parent=Msa0298410-mRNA-1 |
| chr2_3 | CDS | 52373892 | 52375067 | 52373892 | ID=Msa0298410-mRNA-1:cds;Parent=Msa0298410-mRNA-1 |
| Gene Sequence |
| Protein sequence |
