Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0821220 | XP_003617205.1 | 96.667 | 60 | 2 | 0 | 1 | 60 | 24 | 83 | 5.11e-32 | 117 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0821220 | G7KCU3 | 96.667 | 60 | 2 | 0 | 1 | 60 | 24 | 83 | 2.44e-32 | 117 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0015140 | Msa0821220 | 0.800635 | 1.314083e-48 | -8.615850e-47 |
| Msa0026660 | Msa0821220 | 0.825736 | 3.984738e-54 | -8.615850e-47 |
| Msa0029260 | Msa0821220 | 0.810805 | 9.604349e-51 | -8.615850e-47 |
| Msa0030300 | Msa0821220 | 0.807116 | 5.915420e-50 | -8.615850e-47 |
| Msa0033880 | Msa0821220 | 0.829788 | 4.232112e-55 | -8.615850e-47 |
| Msa0037160 | Msa0821220 | 0.807462 | 4.995583e-50 | -8.615850e-47 |
| Msa0057370 | Msa0821220 | 0.805846 | 1.096161e-49 | -8.615850e-47 |
| Msa0077900 | Msa0821220 | 0.800335 | 1.512241e-48 | -8.615850e-47 |
| Msa0081650 | Msa0821220 | 0.802406 | 5.695241e-49 | -8.615850e-47 |
| Msa0603670 | Msa0821220 | 0.823163 | 1.605878e-53 | -8.615850e-47 |
| Msa0609000 | Msa0821220 | 0.807939 | 3.956811e-50 | -8.615850e-47 |
| Msa0610130 | Msa0821220 | 0.817098 | 3.932547e-52 | -8.615850e-47 |
| Msa0614510 | Msa0821220 | 0.803384 | 3.577159e-49 | -8.615850e-47 |
| Msa0619920 | Msa0821220 | 0.822671 | 2.090609e-53 | -8.615850e-47 |
| Msa0620620 | Msa0821220 | 0.823760 | 1.164361e-53 | -8.615850e-47 |
| Msa0682090 | Msa0821220 | 0.822807 | 1.944534e-53 | -8.615850e-47 |
| Msa0682140 | Msa0821220 | 0.823515 | 1.328760e-53 | -8.615850e-47 |
| Msa0687020 | Msa0821220 | 0.800704 | 1.272050e-48 | -8.615850e-47 |
| Msa0694840 | Msa0821220 | 0.800782 | 1.226310e-48 | -8.615850e-47 |
| Msa0703910 | Msa0821220 | 0.809688 | 1.672365e-50 | -8.615850e-47 |
| Msa0706390 | Msa0821220 | 0.801688 | 8.003332e-49 | -8.615850e-47 |
| Msa0706400 | Msa0821220 | 0.804558 | 2.039136e-49 | -8.615850e-47 |
| Msa0715410 | Msa0821220 | 0.806389 | 8.424802e-50 | -8.615850e-47 |
| Msa0745200 | Msa0821220 | 0.831680 | 1.455334e-55 | -8.615850e-47 |
| Msa0777320 | Msa0821220 | 0.815706 | 8.055972e-52 | -8.615850e-47 |
| Msa0780880 | Msa0821220 | 0.823976 | 1.036039e-53 | -8.615850e-47 |
| Msa0781270 | Msa0821220 | 0.833472 | 5.229758e-56 | -8.615850e-47 |
| Msa0786750 | Msa0821220 | 0.812786 | 3.557288e-51 | -8.615850e-47 |
| Msa0821190 | Msa0821220 | 0.854316 | 1.334830e-61 | -8.615850e-47 |
| Msa0821220 | Msa0822500 | 0.800921 | 1.148420e-48 | -8.615850e-47 |
| Msa0821220 | Msa0826760 | 0.814765 | 1.304090e-51 | -8.615850e-47 |
| Msa0821220 | Msa0827280 | 0.817092 | 3.945653e-52 | -8.615850e-47 |
| Msa0821220 | Msa0865570 | 0.805141 | 1.540586e-49 | -8.615850e-47 |
| Msa0821220 | Msa0899350 | 0.808003 | 3.834169e-50 | -8.615850e-47 |
| Msa0821220 | Msa0901320 | 0.801293 | 9.641914e-49 | -8.615850e-47 |
| Msa0821220 | Msa0980020 | 0.810104 | 1.360493e-50 | -8.615850e-47 |
| Msa0821220 | Msa0997430 | 0.807404 | 5.138426e-50 | -8.615850e-47 |
| Msa0821220 | Msa1024750 | 0.803441 | 3.481258e-49 | -8.615850e-47 |
| Msa0821220 | Msa1025940 | 0.810493 | 1.121281e-50 | -8.615850e-47 |
| Msa0821220 | Msa1029970 | 0.811432 | 7.022992e-51 | -8.615850e-47 |
| Msa0821220 | Msa1089320 | 0.829041 | 6.425210e-55 | -8.615850e-47 |
| Msa0821220 | Msa1126850 | 0.807568 | 4.743092e-50 | -8.615850e-47 |
| Msa0821220 | Msa1152520 | 0.805498 | 1.296746e-49 | -8.615850e-47 |
| Msa0821220 | Msa1163540 | 0.813213 | 2.867608e-51 | -8.615850e-47 |
| Msa0821220 | Msa1173170 | 0.803081 | 4.132110e-49 | -8.615850e-47 |
| Msa0821220 | Msa1179980 | 0.800172 | 1.632270e-48 | -8.615850e-47 |
| Msa0821220 | Msa1202580 | 0.804564 | 2.033616e-49 | -8.615850e-47 |
| Msa0821220 | Msa1204630 | 0.805175 | 1.515471e-49 | -8.615850e-47 |
| Msa0821220 | Msa1206310 | 0.804273 | 2.337553e-49 | -8.615850e-47 |
| Msa0821220 | Msa1206360 | 0.806643 | 7.446014e-50 | -8.615850e-47 |
| Msa0821220 | Msa1211830 | 0.805340 | 1.399672e-49 | -8.615850e-47 |
| Msa0821220 | Msa1270620 | 0.802642 | 5.092710e-49 | -8.615850e-47 |
| Msa0821220 | Msa1270690 | 0.801532 | 8.612552e-49 | -8.615850e-47 |
| Msa0821220 | Msa1278900 | 0.800887 | 1.166988e-48 | -8.615850e-47 |
| Msa0821220 | Msa1286610 | 0.805265 | 1.451386e-49 | -8.615850e-47 |
| Msa0821220 | Msa1307180 | 0.812517 | 4.073590e-51 | -8.615850e-47 |
| Msa0821220 | Msa1316360 | 0.814367 | 1.597208e-51 | -8.615850e-47 |
| Msa0821220 | Msa1331350 | 0.826500 | 2.622285e-54 | -8.615850e-47 |
| Msa0821220 | Msa1364210 | 0.830191 | 3.374121e-55 | -8.615850e-47 |
| Msa0821220 | Msa1384870 | 0.827907 | 1.207115e-54 | -8.615850e-47 |
| Msa0821220 | Msa1389850 | 0.850670 | 1.461259e-60 | -8.615850e-47 |
| Msa0821220 | Msa1389880 | 0.848907 | 4.542304e-60 | -8.615850e-47 |
| Msa0821220 | Msa1407190 | 0.819526 | 1.108827e-52 | -8.615850e-47 |
| Msa0821220 | Msa1424780 | 0.812711 | 3.695912e-51 | -8.615850e-47 |
| Msa0821220 | Msa1432130 | 0.820274 | 7.479397e-53 | -8.615850e-47 |
| Msa0821220 | Msa1437210 | 0.812658 | 3.794823e-51 | -8.615850e-47 |
| Msa0127850 | Msa0821220 | 0.819387 | 1.192708e-52 | -8.615850e-47 |
| Msa0131450 | Msa0821220 | 0.805712 | 1.169499e-49 | -8.615850e-47 |
| Msa0167980 | Msa0821220 | 0.805051 | 1.608608e-49 | -8.615850e-47 |
| Msa0169300 | Msa0821220 | 0.811310 | 7.461865e-51 | -8.615850e-47 |
| Msa0174000 | Msa0821220 | 0.822935 | 1.814694e-53 | -8.615850e-47 |
| Msa0177210 | Msa0821220 | 0.805897 | 1.069030e-49 | -8.615850e-47 |
| Msa0177230 | Msa0821220 | 0.820411 | 6.957141e-53 | -8.615850e-47 |
| Msa0185510 | Msa0821220 | 0.807282 | 5.455112e-50 | -8.615850e-47 |
| Msa0197660 | Msa0821220 | 0.800179 | 1.627495e-48 | -8.615850e-47 |
| Msa0205010 | Msa0821220 | 0.802152 | 6.425508e-49 | -8.615850e-47 |
| Msa0221600 | Msa0821220 | 0.801636 | 8.201008e-49 | -8.615850e-47 |
| Msa0378970 | Msa0821220 | 0.800386 | 1.476840e-48 | -8.615850e-47 |
| Msa0385490 | Msa0821220 | 0.809673 | 1.684695e-50 | -8.615850e-47 |
| Msa0428050 | Msa0821220 | 0.823211 | 1.565277e-53 | -8.615850e-47 |
| Msa0437400 | Msa0821220 | 0.804425 | 2.174021e-49 | -8.615850e-47 |
| Msa0439890 | Msa0821220 | 0.807464 | 4.991582e-50 | -8.615850e-47 |
| Msa0468320 | Msa0821220 | 0.801026 | 1.093148e-48 | -8.615850e-47 |
| Msa0474390 | Msa0821220 | 0.808417 | 3.128688e-50 | -8.615850e-47 |
| Msa0239490 | Msa0821220 | 0.803093 | 4.109911e-49 | -8.615850e-47 |
| Msa0239800 | Msa0821220 | 0.809653 | 1.701362e-50 | -8.615850e-47 |
| Msa0296820 | Msa0821220 | 0.806845 | 6.747871e-50 | -8.615850e-47 |
| Msa0317510 | Msa0821220 | 0.801497 | 8.757960e-49 | -8.615850e-47 |
| Msa0339910 | Msa0821220 | 0.801214 | 1.000737e-48 | -8.615850e-47 |
| Msa0505380 | Msa0821220 | 0.803381 | 3.581283e-49 | -8.615850e-47 |
| Msa0510930 | Msa0821220 | 0.800841 | 1.192952e-48 | -8.615850e-47 |
| Msa0517640 | Msa0821220 | 0.800668 | 1.293742e-48 | -8.615850e-47 |
| Msa0518440 | Msa0821220 | 0.810152 | 1.328889e-50 | -8.615850e-47 |
| Msa0518810 | Msa0821220 | 0.810851 | 9.385037e-51 | -8.615850e-47 |
| Msa0546570 | Msa0821220 | 0.818273 | 2.135977e-52 | -8.615850e-47 |
| Msa0550540 | Msa0821220 | 0.803935 | 2.748731e-49 | -8.615850e-47 |
| Msa0550570 | Msa0821220 | 0.820205 | 7.757580e-53 | -8.615850e-47 |
| Msa0562940 | Msa0821220 | 0.807975 | 3.886647e-50 | -8.615850e-47 |
| Msa0597000 | Msa0821220 | 0.804098 | 2.542908e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0821220 | MtrunA17_Chr5g0441411 | 96.667 | 60 | 2 | 0 | 1 | 60 | 24 | 83 | 4.70e-36 | 117 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAAACTAACAGATTCATTT+TGG | 0.150883 | 5_3:+59215570 | Msa0821220:intron |
| AAAGTCAGAGGCTGCTAAAA+AGG | 0.332380 | 5_3:+59215601 | Msa0821220:CDS |
| AGATCCAGGTGTTAATTGTC+TGG | 0.439476 | 5_3:-59215246 | None:intergenic |
| TTAACACCTGGATCTGGATC+TGG | 0.464092 | 5_3:+59215254 | Msa0821220:CDS |
| AGACAATTAACACCTGGATC+TGG | 0.479178 | 5_3:+59215248 | Msa0821220:CDS |
| GCAGATCCAGATCCAGATCC+AGG | 0.505288 | 5_3:-59215260 | None:intergenic |
| TGCATGGAATGAAAAGGATA+AGG | 0.508203 | 5_3:+59215387 | Msa0821220:CDS |
| TATCACTGCAGCTAAATGAA+TGG | 0.519982 | 5_3:+59215359 | Msa0821220:intron |
| ATCTGGATCTGCACAGGAAG+AGG | 0.529978 | 5_3:+59215271 | Msa0821220:CDS |
| ATCTGGATCTGGATCTGCAC+AGG | 0.538020 | 5_3:+59215265 | Msa0821220:CDS |
| GCATGATGCATGGAATGAAA+AGG | 0.553411 | 5_3:+59215381 | Msa0821220:CDS |
| TTGGAACAAGCTAAAGTCAG+AGG | 0.617833 | 5_3:+59215589 | Msa0821220:CDS |
| TAAATGAATGGCATGATGCA+TGG | 0.637004 | 5_3:+59215371 | Msa0821220:CDS |
| TCATCCAGACAATTAACACC+TGG | 0.646730 | 5_3:+59215242 | Msa0821220:CDS |
| AATCAAGGAATCAACAAACA+TGG | 0.667146 | 5_3:+59215418 | Msa0821220:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GATGTATTTGTTGAACAATA+AGG | - | chr5_3:59215456-59215475 | None:intergenic | 25.0% |
| !! | ATTTTGTTACAACAATCAAC+TGG | + | chr5_3:59215507-59215526 | Msa0821220:intron | 25.0% |
| !! | AAAATGAATCTGTTAGTTTG+GGG | - | chr5_3:59215571-59215590 | None:intergenic | 25.0% |
| !! | CAAAATGAATCTGTTAGTTT+GGG | - | chr5_3:59215572-59215591 | None:intergenic | 25.0% |
| !! | GATAAGGATTTTGAGAATCA+AGG | + | chr5_3:59215403-59215422 | Msa0821220:CDS | 30.0% |
| AATCAAGGAATCAACAAACA+TGG | + | chr5_3:59215418-59215437 | Msa0821220:CDS | 30.0% | |
| CCAAAATGAATCTGTTAGTT+TGG | - | chr5_3:59215573-59215592 | None:intergenic | 30.0% | |
| ! | CCAAACTAACAGATTCATTT+TGG | + | chr5_3:59215570-59215589 | Msa0821220:intron | 30.0% |
| TATCACTGCAGCTAAATGAA+TGG | + | chr5_3:59215359-59215378 | Msa0821220:intron | 35.0% | |
| TAAATGAATGGCATGATGCA+TGG | + | chr5_3:59215371-59215390 | Msa0821220:CDS | 35.0% | |
| TGCATGGAATGAAAAGGATA+AGG | + | chr5_3:59215387-59215406 | Msa0821220:CDS | 35.0% | |
| TCATCCAGACAATTAACACC+TGG | + | chr5_3:59215242-59215261 | Msa0821220:CDS | 40.0% | |
| AGATCCAGGTGTTAATTGTC+TGG | - | chr5_3:59215249-59215268 | None:intergenic | 40.0% | |
| AGACAATTAACACCTGGATC+TGG | + | chr5_3:59215248-59215267 | Msa0821220:CDS | 40.0% | |
| GCATGATGCATGGAATGAAA+AGG | + | chr5_3:59215381-59215400 | Msa0821220:CDS | 40.0% | |
| TTGGAACAAGCTAAAGTCAG+AGG | + | chr5_3:59215589-59215608 | Msa0821220:CDS | 40.0% | |
| TTAACACCTGGATCTGGATC+TGG | + | chr5_3:59215254-59215273 | Msa0821220:CDS | 45.0% | |
| ATCTGGATCTGGATCTGCAC+AGG | + | chr5_3:59215265-59215284 | Msa0821220:CDS | 50.0% | |
| ATCTGGATCTGCACAGGAAG+AGG | + | chr5_3:59215271-59215290 | Msa0821220:CDS | 50.0% | |
| TGCACAGGAAGAGGTAGTTG+TGG | + | chr5_3:59215280-59215299 | Msa0821220:intron | 50.0% | |
| GCAGATCCAGATCCAGATCC+AGG | - | chr5_3:59215263-59215282 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_3 | gene | 59215224 | 59215622 | 59215224 | ID=Msa0821220;Name=Msa0821220 |
| chr5_3 | mRNA | 59215224 | 59215622 | 59215224 | ID=Msa0821220-mRNA-1;Parent=Msa0821220;Name=Msa0821220-mRNA-1;_AED=0.15;_eAED=0.15;_QI=0|0|0|1|1|1|3|0|60 |
| chr5_3 | exon | 59215224 | 59215292 | 59215224 | ID=Msa0821220-mRNA-1:exon:18007;Parent=Msa0821220-mRNA-1 |
| chr5_3 | exon | 59215370 | 59215439 | 59215370 | ID=Msa0821220-mRNA-1:exon:18008;Parent=Msa0821220-mRNA-1 |
| chr5_3 | exon | 59215582 | 59215622 | 59215582 | ID=Msa0821220-mRNA-1:exon:18009;Parent=Msa0821220-mRNA-1 |
| chr5_3 | CDS | 59215224 | 59215292 | 59215224 | ID=Msa0821220-mRNA-1:cds;Parent=Msa0821220-mRNA-1 |
| chr5_3 | CDS | 59215370 | 59215439 | 59215370 | ID=Msa0821220-mRNA-1:cds;Parent=Msa0821220-mRNA-1 |
| chr5_3 | CDS | 59215582 | 59215622 | 59215582 | ID=Msa0821220-mRNA-1:cds;Parent=Msa0821220-mRNA-1 |
| Gene Sequence |
| Protein sequence |
