AlfalfaGEDB | Msa0850720

M. sativa cultivar zhongmu-4 / Msa0850720

KEGG: -
GO: GO:0003674 GO:0005215 GO:0005337 GO:0005345 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006863 GO:0008150 GO:0009914 GO:0010184 GO:0010817 GO:0015205 GO:0015211 GO:0015851 GO:0015858 GO:0015860 GO:0015931 GO:0015932 GO:0016020 GO:0016021 GO:0022857 GO:0031224 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0065007 GO:0065008 GO:0071702 GO:0071705 GO:0071944 GO:0072530 GO:1901264 GO:1901505 GO:1901642 GO:1904823
PFAM: PF16913.8

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0850720	XP_003615204.1	94.955	337	12	3	1	337	1	332	0.0	583

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0850720	sp\|Q9FZ95\|PUP3_ARATH	53.918	319	139	3	17	335	1	311	3.02e-121	356

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0850720	G7KBC7	94.955	337	12	3	1	337	1	332	0.0	583

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0005160	Msa0850720	0.834871	2.332036e-56	-8.615850e-47
Msa0006820	Msa0850720	0.807535	4.820438e-50	-8.615850e-47
Msa0008850	Msa0850720	0.840294	9.483768e-58	-8.615850e-47
Msa0018470	Msa0850720	0.818537	1.861538e-52	-8.615850e-47
Msa0047470	Msa0850720	0.839635	1.408429e-57	-8.615850e-47
Msa0055280	Msa0850720	0.805082	1.584628e-49	-8.615850e-47
Msa0057260	Msa0850720	0.824883	6.341792e-54	-8.615850e-47
Msa0057740	Msa0850720	0.829027	6.476593e-55	-8.615850e-47
Msa0067150	Msa0850720	0.807633	4.596233e-50	-8.615850e-47
Msa0080620	Msa0850720	0.802270	6.074594e-49	-8.615850e-47
Msa0098700	Msa0850720	0.815052	1.126111e-51	-8.615850e-47
Msa0103060	Msa0850720	0.844704	6.409580e-59	-8.615850e-47
Msa0606060	Msa0850720	0.806345	8.604478e-50	-8.615850e-47
Msa0607280	Msa0850720	0.820250	7.575676e-53	-8.615850e-47
Msa0610360	Msa0850720	0.801898	7.244503e-49	-8.615850e-47
Msa0615850	Msa0850720	0.819473	1.140114e-52	-8.615850e-47
Msa0617830	Msa0850720	0.822010	2.977754e-53	-8.615850e-47
Msa0618520	Msa0850720	0.805210	1.490048e-49	-8.615850e-47
Msa0620750	Msa0850720	0.802765	4.803694e-49	-8.615850e-47
Msa0650890	Msa0850720	0.800977	1.118732e-48	-8.615850e-47
Msa0653710	Msa0850720	0.810107	1.358447e-50	-8.615850e-47
Msa0656270	Msa0850720	0.805580	1.246183e-49	-8.615850e-47
Msa0656680	Msa0850720	0.821806	3.318883e-53	-8.615850e-47
Msa0686610	Msa0850720	0.812223	4.723429e-51	-8.615850e-47
Msa0690720	Msa0850720	0.800568	1.355823e-48	-8.615850e-47
Msa0698900	Msa0850720	0.834334	3.182784e-56	-8.615850e-47
Msa0724050	Msa0850720	0.803410	3.532090e-49	-8.615850e-47
Msa0732200	Msa0850720	0.804924	1.710493e-49	-8.615850e-47
Msa0739610	Msa0850720	0.808173	3.527655e-50	-8.615850e-47
Msa0763910	Msa0850720	0.804936	1.700650e-49	-8.615850e-47
Msa0773260	Msa0850720	0.809913	1.495879e-50	-8.615850e-47
Msa0773290	Msa0850720	0.879371	1.316070e-69	-8.615850e-47
Msa0793060	Msa0850720	0.801864	7.363859e-49	-8.615850e-47
Msa0822370	Msa0850720	0.820802	5.659100e-53	-8.615850e-47
Msa0824770	Msa0850720	0.800404	1.464197e-48	-8.615850e-47
Msa0149070	Msa0850720	0.803609	3.212401e-49	-8.615850e-47
Msa0157020	Msa0850720	0.812523	4.061423e-51	-8.615850e-47
Msa0158610	Msa0850720	0.816952	4.241720e-52	-8.615850e-47
Msa0200840	Msa0850720	0.812857	3.432015e-51	-8.615850e-47
Msa0203180	Msa0850720	0.839942	1.171749e-57	-8.615850e-47
Msa0210880	Msa0850720	0.837458	5.135949e-57	-8.615850e-47
Msa0216210	Msa0850720	0.803471	3.430789e-49	-8.615850e-47
Msa0225880	Msa0850720	0.803975	2.696289e-49	-8.615850e-47
Msa0230980	Msa0850720	0.827546	1.473611e-54	-8.615850e-47
Msa0234180	Msa0850720	0.825073	5.717793e-54	-8.615850e-47
Msa0417150	Msa0850720	0.825387	4.820242e-54	-8.615850e-47
Msa0421800	Msa0850720	0.831930	1.262777e-55	-8.615850e-47
Msa0427050	Msa0850720	0.804476	2.121540e-49	-8.615850e-47
Msa0456480	Msa0850720	0.818990	1.469013e-52	-8.615850e-47
Msa0466830	Msa0850720	0.809928	1.484835e-50	-8.615850e-47
Msa0467800	Msa0850720	0.835838	1.329185e-56	-8.615850e-47
Msa0467880	Msa0850720	0.836398	9.580840e-57	-8.615850e-47
Msa0243470	Msa0850720	0.809367	1.959990e-50	-8.615850e-47
Msa0246300	Msa0850720	0.831745	1.402732e-55	-8.615850e-47
Msa0260470	Msa0850720	0.807466	4.985754e-50	-8.615850e-47
Msa0265580	Msa0850720	0.807269	5.489739e-50	-8.615850e-47
Msa0270460	Msa0850720	0.836419	9.461641e-57	-8.615850e-47
Msa0281940	Msa0850720	0.804621	1.978686e-49	-8.615850e-47
Msa0284870	Msa0850720	0.817967	2.504896e-52	-8.615850e-47
Msa0304110	Msa0850720	0.815822	7.593331e-52	-8.615850e-47
Msa0309060	Msa0850720	0.824567	7.525538e-54	-8.615850e-47
Msa0341190	Msa0850720	0.816903	4.349308e-52	-8.615850e-47
Msa0358290	Msa0850720	0.801712	7.909543e-49	-8.615850e-47
Msa0362430	Msa0850720	0.815365	9.597735e-52	-8.615850e-47
Msa0514260	Msa0850720	0.829393	5.277638e-55	-8.615850e-47
Msa0534420	Msa0850720	0.830249	3.266133e-55	-8.615850e-47
Msa0553520	Msa0850720	0.807758	4.322760e-50	-8.615850e-47
Msa0558890	Msa0850720	0.806225	9.120047e-50	-8.615850e-47
Msa0568610	Msa0850720	0.810119	1.350652e-50	-8.615850e-47
Msa0583310	Msa0850720	0.824805	6.614314e-54	-8.615850e-47
Msa0850720	Msa0850740	0.942441	7.144724e-102	-8.615850e-47
Msa0850720	Msa0859530	0.809124	2.209478e-50	-8.615850e-47
Msa0850720	Msa0861800	0.802280	6.046537e-49	-8.615850e-47
Msa0850720	Msa0899730	0.815363	9.607121e-52	-8.615850e-47
Msa0850720	Msa0913120	0.809627	1.723359e-50	-8.615850e-47
Msa0850720	Msa0934830	0.828176	1.040055e-54	-8.615850e-47
Msa0850720	Msa0964650	0.844988	5.374087e-59	-8.615850e-47
Msa0850720	Msa0971860	0.831233	1.874247e-55	-8.615850e-47
Msa0850720	Msa0981680	0.827027	1.961918e-54	-8.615850e-47
Msa0850720	Msa1024580	0.808623	2.828101e-50	-8.615850e-47
Msa0850720	Msa1030430	0.813882	2.043531e-51	-8.615850e-47
Msa0850720	Msa1043870	0.803562	3.284863e-49	-8.615850e-47
Msa0850720	Msa1067910	0.818028	2.427540e-52	-8.615850e-47
Msa0850720	Msa1079610	0.800464	1.423458e-48	-8.615850e-47
Msa0850720	Msa1130660	0.800309	1.530887e-48	-8.615850e-47
Msa0850720	Msa1186580	0.810436	1.153995e-50	-8.615850e-47
Msa0850720	Msa1198450	0.864640	1.051477e-64	-8.615850e-47
Msa0850720	Msa1211260	0.813925	1.999565e-51	-8.615850e-47
Msa0850720	Msa1211300	0.805569	1.253097e-49	-8.615850e-47
Msa0850720	Msa1211390	0.829039	6.431719e-55	-8.615850e-47
Msa0850720	Msa1220740	0.822154	2.757160e-53	-8.615850e-47
Msa0850720	Msa1283120	0.833010	6.818164e-56	-8.615850e-47
Msa0850720	Msa1299370	0.823930	1.062139e-53	-8.615850e-47
Msa0850720	Msa1322420	0.820328	7.270135e-53	-8.615850e-47
Msa0850720	Msa1327820	0.822126	2.798364e-53	-8.615850e-47
Msa0850720	Msa1334650	0.813002	3.191125e-51	-8.615850e-47
Msa0850720	Msa1349360	0.806467	8.110193e-50	-8.615850e-47
Msa0850720	Msa1361190	0.808171	3.530633e-50	-8.615850e-47
Msa0850720	Msa1364520	0.805078	1.588335e-49	-8.615850e-47
Msa0850720	Msa1365210	0.808603	2.855663e-50	-8.615850e-47
Msa0850720	Msa1388520	0.827035	1.953899e-54	-8.615850e-47
Msa0850720	Msa1395580	0.801556	8.514843e-49	-8.615850e-47
Msa0850720	Msa1413220	0.804535	2.062113e-49	-8.615850e-47
Msa0850720	Msa1413900	0.801115	1.048687e-48	-8.615850e-47
Msa0850720	Msa1415270	0.864091	1.559789e-64	-8.615850e-47
Msa0850720	Msa1435470	0.827142	1.841709e-54	-8.615850e-47
Msa0850720	Msa1440180	0.825949	3.545672e-54	-8.615850e-47
Msa0850720	Msa1449740	0.824226	9.052177e-54	-8.615850e-47
Msa0850720	Msa1458920	0.825100	5.635439e-54	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0850720	MtrunA17_Chr5g0426921	94.955	337	12	3	1	337	1	332	0.0	583
Msa0850720	MtrunA17_Chr3g0132551	62.997	327	113	4	7	328	4	327	2.76e-147	420
Msa0850720	MtrunA17_Chr3g0132561	53.517	327	110	5	7	328	3	292	7.80e-117	341
Msa0850720	MtrunA17_Chr2g0296491	35.962	317	185	6	7	322	60	359	6.07e-61	200
Msa0850720	MtrunA17_Chr4g0055041	34.228	298	187	3	27	321	42	333	5.90e-52	176
Msa0850720	MtrunA17_Chr2g0283381	33.000	300	193	2	22	321	44	335	1.22e-48	167
Msa0850720	MtrunA17_Chr7g0249781	34.688	320	195	5	6	323	14	321	5.58e-48	165
Msa0850720	MtrunA17_Chr3g0102091	33.127	323	202	4	3	321	30	342	1.30e-47	165
Msa0850720	MtrunA17_Chr8g0346091	32.258	310	183	3	20	323	53	341	1.52e-45	159
Msa0850720	MtrunA17_Chr2g0296481	32.836	335	201	8	7	339	40	352	2.51e-43	154
Msa0850720	MtrunA17_Chr2g0313931	36.719	128	68	2	22	148	34	149	2.00e-16	76.3
Msa0850720	MtrunA17_Chr6g0483671	31.452	124	85	0	198	321	5	128	9.46e-15	71.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0850720	AT2G33750.2	58.117	308	122	2	17	324	3	303	5.97e-123	357
Msa0850720	AT1G28220.1	53.918	319	139	3	17	335	1	311	3.07e-122	356
Msa0850720	AT2G33750.1	56.113	319	122	3	17	324	3	314	2.97e-119	348
Msa0850720	AT1G28230.1	57.508	313	130	3	17	328	1	311	1.34e-116	342
Msa0850720	AT4G18220.1	35.154	293	177	4	32	323	57	337	3.16e-47	164
Msa0850720	AT4G18210.1	32.609	322	197	4	7	323	31	337	1.47e-42	152
Msa0850720	AT4G18205.1	34.828	290	172	4	41	326	61	337	9.76e-42	149
Msa0850720	AT1G30840.2	32.500	320	198	6	7	322	11	316	7.40e-39	142
Msa0850720	AT1G30840.1	32.500	320	198	6	7	322	11	316	7.40e-39	142
Msa0850720	AT4G18197.1	33.103	290	177	4	41	326	64	340	3.22e-38	140
Msa0850720	AT2G24220.1	33.230	322	199	4	2	323	21	326	2.46e-37	137
Msa0850720	AT2G24220.2	33.230	322	199	4	2	323	39	344	3.01e-37	137
Msa0850720	AT1G44750.6	32.692	312	197	4	13	323	24	323	8.92e-37	136
Msa0850720	AT1G44750.2	32.692	312	197	4	13	323	24	323	8.92e-37	136
Msa0850720	AT1G44750.1	32.692	312	197	4	13	323	36	335	1.04e-36	136
Msa0850720	AT1G44750.7	36.406	217	137	1	107	323	44	259	7.58e-36	132
Msa0850720	AT1G44750.5	36.406	217	137	1	107	323	44	259	7.58e-36	132
Msa0850720	AT1G44750.4	36.406	217	137	1	107	323	44	259	7.58e-36	132
Msa0850720	AT1G44750.8	36.406	217	137	1	107	323	44	259	7.58e-36	132
Msa0850720	AT1G44750.3	36.406	217	137	1	107	323	44	259	7.58e-36	132
Msa0850720	AT4G18195.1	31.597	288	184	3	41	326	67	343	2.77e-34	130
Msa0850720	AT4G18190.1	31.046	306	193	5	3	302	20	313	4.29e-33	127
Msa0850720	AT4G08700.1	27.190	331	214	6	5	323	9	324	9.58e-33	125
Msa0850720	AT5G41160.1	26.888	331	218	6	5	323	9	327	3.17e-31	121
Msa0850720	AT1G57943.1	28.470	281	174	7	49	318	85	349	3.33e-21	94.0
Msa0850720	AT1G57943.2	28.470	281	174	7	49	318	84	348	3.57e-21	94.0
Msa0850720	AT1G19770.1	26.510	298	202	5	51	340	88	376	5.58e-20	90.5
Msa0850720	AT1G57980.1	27.500	280	177	6	49	318	82	345	1.26e-17	83.6
Msa0850720	AT1G57990.1	25.418	299	200	7	49	337	78	363	5.10e-17	81.6
Msa0850720	AT1G47603.1	24.484	339	215	9	7	319	24	347	9.12e-15	75.1
Msa0850720	AT1G09860.1	27.551	294	181	12	49	327	69	345	2.82e-13	70.5
Msa0850720	AT1G75470.1	20.874	206	151	3	124	319	144	347	4.67e-11	63.9

Find 77 sgRNAs with CRISPR-Local

Find 284 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCTTTGGTGCTCTCACTTT+GGG	0.109096	5_4:+40440693	Msa0850720:CDS
GCTAAGGGAATGAGAATTAT+TGG	0.211382	5_4:-40439006	None:intergenic
TGTGCCTCTTCATTGCTATC+TGG	0.276545	5_4:+40440585	Msa0850720:CDS
AAAGACAATAGGATAAAATT+TGG	0.295022	5_4:-40440949	None:intergenic
GACCCTTGGGAGTGTTGTTT+TGG	0.297124	5_4:+40439314	Msa0850720:CDS
AACAGCTAAGATTTCAGTTA+TGG	0.309424	5_4:-40440631	None:intergenic
TTGGCTTCTGCTGCTTTGTA+TGG	0.317318	5_4:+40439411	Msa0850720:CDS
TTGCTTTGGTGCTCTCACTT+TGG	0.319968	5_4:+40440692	Msa0850720:CDS
GGATTGAGTTTAGTATTAGA+AGG	0.332698	5_4:-40438879	None:intergenic
TCTTAACATGTAAAGAAGTA+TGG	0.337721	5_4:-40438737	None:intergenic
TATTACTTATTCTCTTGTTA+TGG	0.358957	5_4:+40439482	Msa0850720:CDS
TCTACTAAGGATTATGTGAT+TGG	0.363277	5_4:+40439375	Msa0850720:CDS
AGCAGAGAAAGGGGTTGCTT+TGG	0.380450	5_4:+40440678	Msa0850720:CDS
TTATTATTGTTGTTGCCGTT+AGG	0.386452	5_4:-40439072	None:intergenic
TTGTGGTCATTCCTCAAGTT+GGG	0.388041	5_4:-40440887	None:intergenic
ACAGCTAAGATTTCAGTTAT+GGG	0.401537	5_4:-40440630	None:intergenic
AACGGTAACGGTAACGGTTG+TGG	0.403687	5_4:-40439041	None:intergenic
TATTATTGTTGTTGCCGTTA+GGG	0.409224	5_4:-40439071	None:intergenic
ACCACCTTTGTTTATCGCTT+CGG	0.421218	5_4:+40439113	Msa0850720:CDS
TGAAGTATAGACGCATTACT+AGG	0.425291	5_4:-40438930	None:intergenic
CTAAGGGAATGAGAATTATT+GGG	0.433473	5_4:-40439005	None:intergenic
TTGAAATCCCTTGCTTCCCT+TGG	0.436924	5_4:-40440474	None:intergenic
TTTGTGGTCATTCCTCAAGT+TGG	0.447528	5_4:-40440888	None:intergenic
GAAGCAAGGGATTTCAAGCT+TGG	0.447995	5_4:+40440480	Msa0850720:CDS
GCCATACACGTTTGCCGCCA+TGG	0.460981	5_4:-40438954	None:intergenic
AAGCAAGGGATTTCAAGCTT+GGG	0.461079	5_4:+40440481	Msa0850720:CDS
ATTATTCTTTCCACCATGGC+TGG	0.461751	5_4:-40438814	None:intergenic
TCCATGGCGGCAAACGTGTA+TGG	0.467259	5_4:+40438953	Msa0850720:CDS
ACTTGTTGGTGAGTCTACTA+AGG	0.477683	5_4:+40439362	Msa0850720:CDS
GTGATTGAATGAAGACTTTG+AGG	0.480812	5_4:-40440784	None:intergenic
TTGGGAAGGCAGATGTTTCA+AGG	0.487413	5_4:-40438987	None:intergenic
GGGTCCACCACATGTTCCTA+TGG	0.491438	5_4:-40438908	None:intergenic
ACAATAGGATAAAATTTGGC+GGG	0.497107	5_4:-40440945	None:intergenic
GATGGTGGAGATAGACTTGT+TGG	0.502081	5_4:+40439348	Msa0850720:CDS
TTTATCGCTTCGGCAGTCAT+CGG	0.508139	5_4:+40439123	Msa0850720:CDS
CACATGTTCCTATGGCTAAG+AGG	0.511689	5_4:-40438900	None:intergenic
TTTCGTAACGGTAACGGTAA+CGG	0.513127	5_4:-40439047	None:intergenic
CGATTTCGTAGGTGATTCCA+AGG	0.517543	5_4:+40440457	Msa0850720:intron
GTAGAGGTAGTCGTCTAAAC+CGG	0.524198	5_4:-40439154	None:intergenic
TTATAAACCAACTCCAACAA+CGG	0.529943	5_4:-40439444	None:intergenic
GAAGTATAGACGCATTACTA+GGG	0.537685	5_4:-40438929	None:intergenic
GACAATAGGATAAAATTTGG+CGG	0.542586	5_4:-40440946	None:intergenic
GTTAATTATTCTTTCCACCA+TGG	0.544404	5_4:-40438818	None:intergenic
GCTTTGGTGCTCTCACTTTG+GGG	0.547915	5_4:+40440694	Msa0850720:CDS
ACGGTTGTGGATGTGAGAAA+TGG	0.560217	5_4:-40439028	None:intergenic
CGGTAGACGCTTTATGCCGC+AGG	0.567407	5_4:-40439178	None:intergenic
AGGTGATTCCAAGGGAAGCA+AGG	0.567865	5_4:+40440466	Msa0850720:intron
CTGCGGCATAAAGCGTCTAC+CGG	0.571271	5_4:+40439179	Msa0850720:CDS
TACCGTTACGAAAACCCTAA+CGG	0.577948	5_4:+40439057	Msa0850720:CDS
GCAGTCATCGGAGTTCTCAC+CGG	0.580044	5_4:+40439135	Msa0850720:CDS
AGCAAGGGATTTCAAGCTTG+GGG	0.584822	5_4:+40440482	Msa0850720:CDS
AGTACTATGTTGTGCTAGTG+TGG	0.588131	5_4:+40440511	Msa0850720:CDS
CTCTTAGCCATAGGAACATG+TGG	0.591598	5_4:+40438901	Msa0850720:CDS
CTAGCTTTGTACCCAACTTG+AGG	0.592540	5_4:+40440876	Msa0850720:three_prime_UTR
AACTCAATCCTCTTAGCCAT+AGG	0.604730	5_4:+40438892	Msa0850720:CDS
ATGCGTCTATACTTCATCCA+TGG	0.605124	5_4:+40438937	Msa0850720:CDS
GATACCAGATAGCAATGAAG+AGG	0.606353	5_4:-40440589	None:intergenic
GTGCCAAAACAACACTCCCA+AGG	0.608152	5_4:-40439317	None:intergenic
TGCCAAAACAACACTCCCAA+GGG	0.608894	5_4:-40439316	None:intergenic
GTGGATGTGAGAAATGGCTA+AGG	0.612500	5_4:-40439022	None:intergenic
GGTGATTCCAAGGGAAGCAA+GGG	0.622275	5_4:+40440467	Msa0850720:intron
CAATAGGATAAAATTTGGCG+GGG	0.623803	5_4:-40440944	None:intergenic
CGCGGTGAAAGCTAAGTGTG+AGG	0.632940	5_4:-40439226	None:intergenic
GGGAATGAGAATTATTGGGA+AGG	0.638688	5_4:-40439001	None:intergenic
GCTTTATGCCGCAGGCGTAG+AGG	0.652914	5_4:-40439170	None:intergenic
TTGGCACTGAATTCTGATGG+TGG	0.661099	5_4:+40439333	Msa0850720:CDS
GACGACTACCTCTACGCCTG+CGG	0.669034	5_4:+40439162	Msa0850720:CDS
TGGATGTGAGAAATGGCTAA+GGG	0.669361	5_4:-40439021	None:intergenic
AAGTATAGACGCATTACTAG+GGG	0.673904	5_4:-40438928	None:intergenic
TTAGCCATAGGAACATGTGG+TGG	0.677866	5_4:+40438904	Msa0850720:CDS
ACTGCCGAAGCGATAAACAA+AGG	0.695167	5_4:-40439117	None:intergenic
ATTAACGAAGAAGTTGACAC+CGG	0.698512	5_4:-40439198	None:intergenic
GCCGAAGCGATAAACAAAGG+TGG	0.698965	5_4:-40439114	None:intergenic
CGTCTATACTTCATCCATGG+CGG	0.706082	5_4:+40438940	Msa0850720:CDS
GATTTCGTAGGTGATTCCAA+GGG	0.713597	5_4:+40440458	Msa0850720:intron
TAGTGTGGAGTGCTATAATG+TGG	0.749239	5_4:+40440526	Msa0850720:CDS
CTCACACTTAGCTTTCACCG+CGG	0.755684	5_4:+40439227	Msa0850720:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GTTAAAAGTTTAAATAAAAA+AGG	-	chr5_4:40439698-40439717	None:intergenic	10.0%
!!	AAAAATATAGAATTAGAATA+AGG	+	chr5_4:40439900-40439919	Msa0850720:intron	10.0%
!!	GTTAAAAGTTTAAATAAAAA+AGG	-	chr5_4:40439698-40439717	None:intergenic	10.0%
!!	AAAAATATAGAATTAGAATA+AGG	+	chr5_4:40439900-40439919	Msa0850720:intron	10.0%
!!	AATAAAAAAGGAAAAAAACA+TGG	-	chr5_4:40439686-40439705	None:intergenic	15.0%
!!	ATCAATAATTCAATATCAAT+GGG	-	chr5_4:40439967-40439986	None:intergenic	15.0%
!!	CTAAGAATAATTATTACATT+TGG	+	chr5_4:40440023-40440042	Msa0850720:intron	15.0%
!!!	AAATTTAAAACCTAAATTGT+GGG	-	chr5_4:40440099-40440118	None:intergenic	15.0%
!!!	AAAATTTAAAACCTAAATTG+TGG	-	chr5_4:40440100-40440119	None:intergenic	15.0%
!!!	AAAAGTTTCTTTTAATTGTA+AGG	+	chr5_4:40440142-40440161	Msa0850720:intron	15.0%
!!	TATTACTAATGTAAAACATA+AGG	-	chr5_4:40440426-40440445	None:intergenic	15.0%
!!	AAAAATGAAAAATAGAGAAA+TGG	+	chr5_4:40440756-40440775	Msa0850720:three_prime_UTR	15.0%
!!	AATTAAAACAAAAAGACAAT+AGG	-	chr5_4:40440963-40440982	None:intergenic	15.0%
!!!	TTGTCTTTTTGTTTTAATTT+TGG	+	chr5_4:40440964-40440983	Msa0850720:three_prime_UTR	15.0%
!!!	TGTCTTTTTGTTTTAATTTT+GGG	+	chr5_4:40440965-40440984	Msa0850720:three_prime_UTR	15.0%
!!	AATAAAAAAGGAAAAAAACA+TGG	-	chr5_4:40439686-40439705	None:intergenic	15.0%
!!	ATCAATAATTCAATATCAAT+GGG	-	chr5_4:40439967-40439986	None:intergenic	15.0%
!!	CTAAGAATAATTATTACATT+TGG	+	chr5_4:40440023-40440042	Msa0850720:intron	15.0%
!!!	AAATTTAAAACCTAAATTGT+GGG	-	chr5_4:40440099-40440118	None:intergenic	15.0%
!!!	AAAATTTAAAACCTAAATTG+TGG	-	chr5_4:40440100-40440119	None:intergenic	15.0%
!!!	AAAAGTTTCTTTTAATTGTA+AGG	+	chr5_4:40440142-40440161	Msa0850720:intron	15.0%
!!	TATTACTAATGTAAAACATA+AGG	-	chr5_4:40440426-40440445	None:intergenic	15.0%
!!	AAAAATGAAAAATAGAGAAA+TGG	+	chr5_4:40440756-40440775	Msa0850720:three_prime_UTR	15.0%
!!	AATTAAAACAAAAAGACAAT+AGG	-	chr5_4:40440963-40440982	None:intergenic	15.0%
!!!	TTGTCTTTTTGTTTTAATTT+TGG	+	chr5_4:40440964-40440983	Msa0850720:three_prime_UTR	15.0%
!!!	TGTCTTTTTGTTTTAATTTT+GGG	+	chr5_4:40440965-40440984	Msa0850720:three_prime_UTR	15.0%
!!	TATTACTTATTCTCTTGTTA+TGG	+	chr5_4:40439482-40439501	Msa0850720:CDS	20.0%
!!	ATAACAGAATTAGAATATTG+TGG	+	chr5_4:40439870-40439889	Msa0850720:intron	20.0%
!!	CATCAATAATTCAATATCAA+TGG	-	chr5_4:40439968-40439987	None:intergenic	20.0%
!!!	AGATGAGTTTATATTTTATG+CGG	-	chr5_4:40440238-40440257	None:intergenic	20.0%
!!	AATAAACACAATGTAAAACA+TGG	-	chr5_4:40440280-40440299	None:intergenic	20.0%
!!!	GTAATGATGTTATTTTTGTT+TGG	+	chr5_4:40440817-40440836	Msa0850720:three_prime_UTR	20.0%
!!!	TTTTGAATTGTCAATATTGT+AGG	+	chr5_4:40440853-40440872	Msa0850720:three_prime_UTR	20.0%
!!!	CAAAATGTCTTAATATTTTG+TGG	-	chr5_4:40440907-40440926	None:intergenic	20.0%
!!	AAAGACAATAGGATAAAATT+TGG	-	chr5_4:40440952-40440971	None:intergenic	20.0%
!!	TATTACTTATTCTCTTGTTA+TGG	+	chr5_4:40439482-40439501	Msa0850720:CDS	20.0%
!!	ATAACAGAATTAGAATATTG+TGG	+	chr5_4:40439870-40439889	Msa0850720:intron	20.0%
!!	CATCAATAATTCAATATCAA+TGG	-	chr5_4:40439968-40439987	None:intergenic	20.0%
!!!	AGATGAGTTTATATTTTATG+CGG	-	chr5_4:40440238-40440257	None:intergenic	20.0%
!!	AATAAACACAATGTAAAACA+TGG	-	chr5_4:40440280-40440299	None:intergenic	20.0%
!!!	GTAATGATGTTATTTTTGTT+TGG	+	chr5_4:40440817-40440836	Msa0850720:three_prime_UTR	20.0%
!!!	TTTTGAATTGTCAATATTGT+AGG	+	chr5_4:40440853-40440872	Msa0850720:three_prime_UTR	20.0%
!!!	CAAAATGTCTTAATATTTTG+TGG	-	chr5_4:40440907-40440926	None:intergenic	20.0%
!!	AAAGACAATAGGATAAAATT+TGG	-	chr5_4:40440952-40440971	None:intergenic	20.0%
!	TCTTAACATGTAAAGAAGTA+TGG	-	chr5_4:40438740-40438759	None:intergenic	25.0%
!!!	TTAATTTTTTGCTAGAGTAG+AGG	-	chr5_4:40438794-40438813	None:intergenic	25.0%
!!!	GATTGGTTTTGTTATGATAT+TGG	+	chr5_4:40439392-40439411	Msa0850720:CDS	25.0%
!!	GATCATAGACAATGATTTTA+AGG	+	chr5_4:40439557-40439576	Msa0850720:CDS	25.0%
!	TAAGAATAACACACATTCTA+AGG	-	chr5_4:40439813-40439832	None:intergenic	25.0%
!	TTATTCTTAGATTCAATACG+TGG	-	chr5_4:40440013-40440032	None:intergenic	25.0%
!	ATTCCAACTTAATTTACGTA+AGG	+	chr5_4:40440057-40440076	Msa0850720:intron	25.0%
!	AATCCTTACGTAAATTAAGT+TGG	-	chr5_4:40440063-40440082	None:intergenic	25.0%
!!!	GTTTCTTTTAATTGTAAGGT+AGG	+	chr5_4:40440146-40440165	Msa0850720:intron	25.0%
!	ATATGTATAGTAAGTTGTTG+TGG	-	chr5_4:40440373-40440392	None:intergenic	25.0%
!	TCTTAACATGTAAAGAAGTA+TGG	-	chr5_4:40438740-40438759	None:intergenic	25.0%
!!!	TTAATTTTTTGCTAGAGTAG+AGG	-	chr5_4:40438794-40438813	None:intergenic	25.0%
!!!	GATTGGTTTTGTTATGATAT+TGG	+	chr5_4:40439392-40439411	Msa0850720:CDS	25.0%
!!	GATCATAGACAATGATTTTA+AGG	+	chr5_4:40439557-40439576	Msa0850720:CDS	25.0%
!	TAAGAATAACACACATTCTA+AGG	-	chr5_4:40439813-40439832	None:intergenic	25.0%
!	TTATTCTTAGATTCAATACG+TGG	-	chr5_4:40440013-40440032	None:intergenic	25.0%
!	ATTCCAACTTAATTTACGTA+AGG	+	chr5_4:40440057-40440076	Msa0850720:intron	25.0%
!	AATCCTTACGTAAATTAAGT+TGG	-	chr5_4:40440063-40440082	None:intergenic	25.0%
!!!	GTTTCTTTTAATTGTAAGGT+AGG	+	chr5_4:40440146-40440165	Msa0850720:intron	25.0%
!	ATATGTATAGTAAGTTGTTG+TGG	-	chr5_4:40440373-40440392	None:intergenic	25.0%
	GTTAATTATTCTTTCCACCA+TGG	-	chr5_4:40438821-40438840	None:intergenic	30.0%
	GGATTGAGTTTAGTATTAGA+AGG	-	chr5_4:40438882-40438901	None:intergenic	30.0%
	CTAAGGGAATGAGAATTATT+GGG	-	chr5_4:40439008-40439027	None:intergenic	30.0%
!	TATTATTGTTGTTGCCGTTA+GGG	-	chr5_4:40439074-40439093	None:intergenic	30.0%
!	TTATTATTGTTGTTGCCGTT+AGG	-	chr5_4:40439075-40439094	None:intergenic	30.0%
	TCTACTAAGGATTATGTGAT+TGG	+	chr5_4:40439375-40439394	Msa0850720:CDS	30.0%
	TTATAAACCAACTCCAACAA+CGG	-	chr5_4:40439447-40439466	None:intergenic	30.0%
	AATTGTGAGTGTACAAAGTA+TGG	-	chr5_4:40439623-40439642	None:intergenic	30.0%
	AAAACACGTGTACATTTGTT+TGG	-	chr5_4:40439774-40439793	None:intergenic	30.0%
	ACAATGTAAAACATGGAAGT+CGG	-	chr5_4:40440273-40440292	None:intergenic	30.0%
!	ATGTGGCAATTTTTCTTCTT+AGG	+	chr5_4:40440543-40440562	Msa0850720:CDS	30.0%
	ACAGCTAAGATTTCAGTTAT+GGG	-	chr5_4:40440633-40440652	None:intergenic	30.0%
	AACAGCTAAGATTTCAGTTA+TGG	-	chr5_4:40440634-40440653	None:intergenic	30.0%
!!	AGAAATTTTCAAGCAGAGAA+AGG	+	chr5_4:40440667-40440686	Msa0850720:CDS	30.0%
!!	GAAATTTTCAAGCAGAGAAA+GGG	+	chr5_4:40440668-40440687	Msa0850720:CDS	30.0%
!!	AAATTTTCAAGCAGAGAAAG+GGG	+	chr5_4:40440669-40440688	Msa0850720:CDS	30.0%
	ACAATAGGATAAAATTTGGC+GGG	-	chr5_4:40440948-40440967	None:intergenic	30.0%
	GACAATAGGATAAAATTTGG+CGG	-	chr5_4:40440949-40440968	None:intergenic	30.0%
	GTTAATTATTCTTTCCACCA+TGG	-	chr5_4:40438821-40438840	None:intergenic	30.0%
	GGATTGAGTTTAGTATTAGA+AGG	-	chr5_4:40438882-40438901	None:intergenic	30.0%
	CTAAGGGAATGAGAATTATT+GGG	-	chr5_4:40439008-40439027	None:intergenic	30.0%
!	TATTATTGTTGTTGCCGTTA+GGG	-	chr5_4:40439074-40439093	None:intergenic	30.0%
!	TTATTATTGTTGTTGCCGTT+AGG	-	chr5_4:40439075-40439094	None:intergenic	30.0%
	TCTACTAAGGATTATGTGAT+TGG	+	chr5_4:40439375-40439394	Msa0850720:CDS	30.0%
	TTATAAACCAACTCCAACAA+CGG	-	chr5_4:40439447-40439466	None:intergenic	30.0%
	AATTGTGAGTGTACAAAGTA+TGG	-	chr5_4:40439623-40439642	None:intergenic	30.0%
	AAAACACGTGTACATTTGTT+TGG	-	chr5_4:40439774-40439793	None:intergenic	30.0%
	ACAATGTAAAACATGGAAGT+CGG	-	chr5_4:40440273-40440292	None:intergenic	30.0%
!	ATGTGGCAATTTTTCTTCTT+AGG	+	chr5_4:40440543-40440562	Msa0850720:CDS	30.0%
	ACAGCTAAGATTTCAGTTAT+GGG	-	chr5_4:40440633-40440652	None:intergenic	30.0%
	AACAGCTAAGATTTCAGTTA+TGG	-	chr5_4:40440634-40440653	None:intergenic	30.0%
!!	AGAAATTTTCAAGCAGAGAA+AGG	+	chr5_4:40440667-40440686	Msa0850720:CDS	30.0%
!!	GAAATTTTCAAGCAGAGAAA+GGG	+	chr5_4:40440668-40440687	Msa0850720:CDS	30.0%
!!	AAATTTTCAAGCAGAGAAAG+GGG	+	chr5_4:40440669-40440688	Msa0850720:CDS	30.0%
	ACAATAGGATAAAATTTGGC+GGG	-	chr5_4:40440948-40440967	None:intergenic	30.0%
	GACAATAGGATAAAATTTGG+CGG	-	chr5_4:40440949-40440968	None:intergenic	30.0%
	AGCAAAAAATTAACCAGCCA+TGG	+	chr5_4:40438801-40438820	Msa0850720:exon	35.0%
	AAAAAATTAACCAGCCATGG+TGG	+	chr5_4:40438804-40438823	Msa0850720:exon	35.0%
	AAGTATAGACGCATTACTAG+GGG	-	chr5_4:40438931-40438950	None:intergenic	35.0%
	GAAGTATAGACGCATTACTA+GGG	-	chr5_4:40438932-40438951	None:intergenic	35.0%
	TGAAGTATAGACGCATTACT+AGG	-	chr5_4:40438933-40438952	None:intergenic	35.0%
	GCTAAGGGAATGAGAATTAT+TGG	-	chr5_4:40439009-40439028	None:intergenic	35.0%
	ATTAACGAAGAAGTTGACAC+CGG	-	chr5_4:40439201-40439220	None:intergenic	35.0%
!!	TCTATTGTTTTGTTGACCCT+TGG	+	chr5_4:40439300-40439319	Msa0850720:CDS	35.0%
!!	CTATTGTTTTGTTGACCCTT+GGG	+	chr5_4:40439301-40439320	Msa0850720:CDS	35.0%
!!!	TGGATTTGTTTTACCGTTGT+TGG	+	chr5_4:40439431-40439450	Msa0850720:CDS	35.0%
!!!	CTACTTTGTTTTGCGTTGTT+GGG	+	chr5_4:40439532-40439551	Msa0850720:CDS	35.0%
	AATTGTGAGTGTACAAAGCA+TGG	-	chr5_4:40439720-40439739	None:intergenic	35.0%
	GTACATTTGTTTGGTTACTG+AGG	-	chr5_4:40439765-40439784	None:intergenic	35.0%
!!!	AGTTTTGAGAAACAGAGAGA+GGG	-	chr5_4:40439934-40439953	None:intergenic	35.0%
	CTAAATTGTGGGACTAAGAT+TGG	-	chr5_4:40440088-40440107	None:intergenic	35.0%
	CAATCTTAGTCCCACAATTT+AGG	+	chr5_4:40440086-40440105	Msa0850720:intron	35.0%
!	AATGAGTATTGCGATTTCGT+AGG	+	chr5_4:40440446-40440465	Msa0850720:intron	35.0%
!	TTTTTCTTCTTAGGAGCGAT+TGG	+	chr5_4:40440552-40440571	Msa0850720:CDS	35.0%
	GTGATTGAATGAAGACTTTG+AGG	-	chr5_4:40440787-40440806	None:intergenic	35.0%
	CAATAGGATAAAATTTGGCG+GGG	-	chr5_4:40440947-40440966	None:intergenic	35.0%
	AGCAAAAAATTAACCAGCCA+TGG	+	chr5_4:40438801-40438820	Msa0850720:exon	35.0%
	AAAAAATTAACCAGCCATGG+TGG	+	chr5_4:40438804-40438823	Msa0850720:exon	35.0%
	AAGTATAGACGCATTACTAG+GGG	-	chr5_4:40438931-40438950	None:intergenic	35.0%
	GAAGTATAGACGCATTACTA+GGG	-	chr5_4:40438932-40438951	None:intergenic	35.0%
	TGAAGTATAGACGCATTACT+AGG	-	chr5_4:40438933-40438952	None:intergenic	35.0%
	GCTAAGGGAATGAGAATTAT+TGG	-	chr5_4:40439009-40439028	None:intergenic	35.0%
	ATTAACGAAGAAGTTGACAC+CGG	-	chr5_4:40439201-40439220	None:intergenic	35.0%
!!	TCTATTGTTTTGTTGACCCT+TGG	+	chr5_4:40439300-40439319	Msa0850720:CDS	35.0%
!!	CTATTGTTTTGTTGACCCTT+GGG	+	chr5_4:40439301-40439320	Msa0850720:CDS	35.0%
!!!	TGGATTTGTTTTACCGTTGT+TGG	+	chr5_4:40439431-40439450	Msa0850720:CDS	35.0%
!!!	CTACTTTGTTTTGCGTTGTT+GGG	+	chr5_4:40439532-40439551	Msa0850720:CDS	35.0%
	AATTGTGAGTGTACAAAGCA+TGG	-	chr5_4:40439720-40439739	None:intergenic	35.0%
	GTACATTTGTTTGGTTACTG+AGG	-	chr5_4:40439765-40439784	None:intergenic	35.0%
!!!	AGTTTTGAGAAACAGAGAGA+GGG	-	chr5_4:40439934-40439953	None:intergenic	35.0%
	CTAAATTGTGGGACTAAGAT+TGG	-	chr5_4:40440088-40440107	None:intergenic	35.0%
	CAATCTTAGTCCCACAATTT+AGG	+	chr5_4:40440086-40440105	Msa0850720:intron	35.0%
!	AATGAGTATTGCGATTTCGT+AGG	+	chr5_4:40440446-40440465	Msa0850720:intron	35.0%
!	TTTTTCTTCTTAGGAGCGAT+TGG	+	chr5_4:40440552-40440571	Msa0850720:CDS	35.0%
	GTGATTGAATGAAGACTTTG+AGG	-	chr5_4:40440787-40440806	None:intergenic	35.0%
	CAATAGGATAAAATTTGGCG+GGG	-	chr5_4:40440947-40440966	None:intergenic	35.0%
	ATTATTCTTTCCACCATGGC+TGG	-	chr5_4:40438817-40438836	None:intergenic	40.0%
	AACTCAATCCTCTTAGCCAT+AGG	+	chr5_4:40438892-40438911	Msa0850720:CDS	40.0%
	ATGCGTCTATACTTCATCCA+TGG	+	chr5_4:40438937-40438956	Msa0850720:CDS	40.0%
	GGGAATGAGAATTATTGGGA+AGG	-	chr5_4:40439004-40439023	None:intergenic	40.0%
	TGGATGTGAGAAATGGCTAA+GGG	-	chr5_4:40439024-40439043	None:intergenic	40.0%
!	TTTCGTAACGGTAACGGTAA+CGG	-	chr5_4:40439050-40439069	None:intergenic	40.0%
!	TAGGGTTTTCGTAACGGTAA+CGG	-	chr5_4:40439056-40439075	None:intergenic	40.0%
	TACCGTTACGAAAACCCTAA+CGG	+	chr5_4:40439057-40439076	Msa0850720:CDS	40.0%
!	ACCACCTTTGTTTATCGCTT+CGG	+	chr5_4:40439113-40439132	Msa0850720:CDS	40.0%
	AAGAAAAAACGCGAAAACCG+CGG	-	chr5_4:40439247-40439266	None:intergenic	40.0%
!!	GTTTTGGCACTGAATTCTGA+TGG	+	chr5_4:40439330-40439349	Msa0850720:CDS	40.0%
!	ACTTGTTGGTGAGTCTACTA+AGG	+	chr5_4:40439362-40439381	Msa0850720:CDS	40.0%
!	TGTTTTACCGTTGTTGGAGT+TGG	+	chr5_4:40439437-40439456	Msa0850720:CDS	40.0%
!!!	GCTACTTTGTTTTGCGTTGT+TGG	+	chr5_4:40439531-40439550	Msa0850720:CDS	40.0%
!!	TTTTGAGAAACAGAGAGAGG+GGG	-	chr5_4:40439932-40439951	None:intergenic	40.0%
!!!	GTTTTGAGAAACAGAGAGAG+GGG	-	chr5_4:40439933-40439952	None:intergenic	40.0%
!!!	GAGTTTTGAGAAACAGAGAG+AGG	-	chr5_4:40439935-40439954	None:intergenic	40.0%
	GATTTCGTAGGTGATTCCAA+GGG	+	chr5_4:40440458-40440477	Msa0850720:intron	40.0%
	AAGCAAGGGATTTCAAGCTT+GGG	+	chr5_4:40440481-40440500	Msa0850720:CDS	40.0%
	AGTACTATGTTGTGCTAGTG+TGG	+	chr5_4:40440511-40440530	Msa0850720:CDS	40.0%
!	TAGTGTGGAGTGCTATAATG+TGG	+	chr5_4:40440526-40440545	Msa0850720:CDS	40.0%
	GATACCAGATAGCAATGAAG+AGG	-	chr5_4:40440592-40440611	None:intergenic	40.0%
	TTGTGGTCATTCCTCAAGTT+GGG	-	chr5_4:40440890-40440909	None:intergenic	40.0%
	TTTGTGGTCATTCCTCAAGT+TGG	-	chr5_4:40440891-40440910	None:intergenic	40.0%
	ATTATTCTTTCCACCATGGC+TGG	-	chr5_4:40438817-40438836	None:intergenic	40.0%
	AACTCAATCCTCTTAGCCAT+AGG	+	chr5_4:40438892-40438911	Msa0850720:CDS	40.0%
	ATGCGTCTATACTTCATCCA+TGG	+	chr5_4:40438937-40438956	Msa0850720:CDS	40.0%
	GGGAATGAGAATTATTGGGA+AGG	-	chr5_4:40439004-40439023	None:intergenic	40.0%
	TGGATGTGAGAAATGGCTAA+GGG	-	chr5_4:40439024-40439043	None:intergenic	40.0%
!	TTTCGTAACGGTAACGGTAA+CGG	-	chr5_4:40439050-40439069	None:intergenic	40.0%
!	TAGGGTTTTCGTAACGGTAA+CGG	-	chr5_4:40439056-40439075	None:intergenic	40.0%
	TACCGTTACGAAAACCCTAA+CGG	+	chr5_4:40439057-40439076	Msa0850720:CDS	40.0%
!	ACCACCTTTGTTTATCGCTT+CGG	+	chr5_4:40439113-40439132	Msa0850720:CDS	40.0%
	AAGAAAAAACGCGAAAACCG+CGG	-	chr5_4:40439247-40439266	None:intergenic	40.0%
!!	GTTTTGGCACTGAATTCTGA+TGG	+	chr5_4:40439330-40439349	Msa0850720:CDS	40.0%
!	ACTTGTTGGTGAGTCTACTA+AGG	+	chr5_4:40439362-40439381	Msa0850720:CDS	40.0%
!	TGTTTTACCGTTGTTGGAGT+TGG	+	chr5_4:40439437-40439456	Msa0850720:CDS	40.0%
!!!	GCTACTTTGTTTTGCGTTGT+TGG	+	chr5_4:40439531-40439550	Msa0850720:CDS	40.0%
!!	TTTTGAGAAACAGAGAGAGG+GGG	-	chr5_4:40439932-40439951	None:intergenic	40.0%
!!!	GTTTTGAGAAACAGAGAGAG+GGG	-	chr5_4:40439933-40439952	None:intergenic	40.0%
!!!	GAGTTTTGAGAAACAGAGAG+AGG	-	chr5_4:40439935-40439954	None:intergenic	40.0%
	GATTTCGTAGGTGATTCCAA+GGG	+	chr5_4:40440458-40440477	Msa0850720:intron	40.0%
	AAGCAAGGGATTTCAAGCTT+GGG	+	chr5_4:40440481-40440500	Msa0850720:CDS	40.0%
	AGTACTATGTTGTGCTAGTG+TGG	+	chr5_4:40440511-40440530	Msa0850720:CDS	40.0%
!	TAGTGTGGAGTGCTATAATG+TGG	+	chr5_4:40440526-40440545	Msa0850720:CDS	40.0%
	GATACCAGATAGCAATGAAG+AGG	-	chr5_4:40440592-40440611	None:intergenic	40.0%
	TTGTGGTCATTCCTCAAGTT+GGG	-	chr5_4:40440890-40440909	None:intergenic	40.0%
	TTTGTGGTCATTCCTCAAGT+TGG	-	chr5_4:40440891-40440910	None:intergenic	40.0%
	CACATGTTCCTATGGCTAAG+AGG	-	chr5_4:40438903-40438922	None:intergenic	45.0%
	CTCTTAGCCATAGGAACATG+TGG	+	chr5_4:40438901-40438920	Msa0850720:CDS	45.0%
	TTAGCCATAGGAACATGTGG+TGG	+	chr5_4:40438904-40438923	Msa0850720:CDS	45.0%
	CGTCTATACTTCATCCATGG+CGG	+	chr5_4:40438940-40438959	Msa0850720:CDS	45.0%
	TTGGGAAGGCAGATGTTTCA+AGG	-	chr5_4:40438990-40439009	None:intergenic	45.0%
	GTGGATGTGAGAAATGGCTA+AGG	-	chr5_4:40439025-40439044	None:intergenic	45.0%
	ACGGTTGTGGATGTGAGAAA+TGG	-	chr5_4:40439031-40439050	None:intergenic	45.0%
!	TGCCGTTAGGGTTTTCGTAA+CGG	-	chr5_4:40439062-40439081	None:intergenic	45.0%
	ACTGCCGAAGCGATAAACAA+AGG	-	chr5_4:40439120-40439139	None:intergenic	45.0%
	TTTATCGCTTCGGCAGTCAT+CGG	+	chr5_4:40439123-40439142	Msa0850720:CDS	45.0%
	GTAGAGGTAGTCGTCTAAAC+CGG	-	chr5_4:40439157-40439176	None:intergenic	45.0%
	TGCCAAAACAACACTCCCAA+GGG	-	chr5_4:40439319-40439338	None:intergenic	45.0%
!	TTGGCACTGAATTCTGATGG+TGG	+	chr5_4:40439333-40439352	Msa0850720:CDS	45.0%
!!	GATGGTGGAGATAGACTTGT+TGG	+	chr5_4:40439348-40439367	Msa0850720:CDS	45.0%
!!	TTGGCTTCTGCTGCTTTGTA+TGG	+	chr5_4:40439411-40439430	Msa0850720:CDS	45.0%
	TTTGAGAAACAGAGAGAGGG+GGG	-	chr5_4:40439931-40439950	None:intergenic	45.0%
	CGGTAAGAAAATGTGCAGTG+AGG	-	chr5_4:40440218-40440237	None:intergenic	45.0%
	CGATTTCGTAGGTGATTCCA+AGG	+	chr5_4:40440457-40440476	Msa0850720:intron	45.0%
	TTGAAATCCCTTGCTTCCCT+TGG	-	chr5_4:40440477-40440496	None:intergenic	45.0%
	GAAGCAAGGGATTTCAAGCT+TGG	+	chr5_4:40440480-40440499	Msa0850720:CDS	45.0%
	AGCAAGGGATTTCAAGCTTG+GGG	+	chr5_4:40440482-40440501	Msa0850720:CDS	45.0%
	TGTGCCTCTTCATTGCTATC+TGG	+	chr5_4:40440585-40440604	Msa0850720:CDS	45.0%
!!	TTGCTTTGGTGCTCTCACTT+TGG	+	chr5_4:40440692-40440711	Msa0850720:CDS	45.0%
!!	TGCTTTGGTGCTCTCACTTT+GGG	+	chr5_4:40440693-40440712	Msa0850720:CDS	45.0%
!!	CTAGCTTTGTACCCAACTTG+AGG	+	chr5_4:40440876-40440895	Msa0850720:three_prime_UTR	45.0%
	CACATGTTCCTATGGCTAAG+AGG	-	chr5_4:40438903-40438922	None:intergenic	45.0%
	CTCTTAGCCATAGGAACATG+TGG	+	chr5_4:40438901-40438920	Msa0850720:CDS	45.0%
	TTAGCCATAGGAACATGTGG+TGG	+	chr5_4:40438904-40438923	Msa0850720:CDS	45.0%
	CGTCTATACTTCATCCATGG+CGG	+	chr5_4:40438940-40438959	Msa0850720:CDS	45.0%
	TTGGGAAGGCAGATGTTTCA+AGG	-	chr5_4:40438990-40439009	None:intergenic	45.0%
	GTGGATGTGAGAAATGGCTA+AGG	-	chr5_4:40439025-40439044	None:intergenic	45.0%
	ACGGTTGTGGATGTGAGAAA+TGG	-	chr5_4:40439031-40439050	None:intergenic	45.0%
!	TGCCGTTAGGGTTTTCGTAA+CGG	-	chr5_4:40439062-40439081	None:intergenic	45.0%
	ACTGCCGAAGCGATAAACAA+AGG	-	chr5_4:40439120-40439139	None:intergenic	45.0%
	TTTATCGCTTCGGCAGTCAT+CGG	+	chr5_4:40439123-40439142	Msa0850720:CDS	45.0%
	GTAGAGGTAGTCGTCTAAAC+CGG	-	chr5_4:40439157-40439176	None:intergenic	45.0%
	TGCCAAAACAACACTCCCAA+GGG	-	chr5_4:40439319-40439338	None:intergenic	45.0%
!	TTGGCACTGAATTCTGATGG+TGG	+	chr5_4:40439333-40439352	Msa0850720:CDS	45.0%
!!	GATGGTGGAGATAGACTTGT+TGG	+	chr5_4:40439348-40439367	Msa0850720:CDS	45.0%
!!	TTGGCTTCTGCTGCTTTGTA+TGG	+	chr5_4:40439411-40439430	Msa0850720:CDS	45.0%
	TTTGAGAAACAGAGAGAGGG+GGG	-	chr5_4:40439931-40439950	None:intergenic	45.0%
	CGGTAAGAAAATGTGCAGTG+AGG	-	chr5_4:40440218-40440237	None:intergenic	45.0%
	CGATTTCGTAGGTGATTCCA+AGG	+	chr5_4:40440457-40440476	Msa0850720:intron	45.0%
	TTGAAATCCCTTGCTTCCCT+TGG	-	chr5_4:40440477-40440496	None:intergenic	45.0%
	GAAGCAAGGGATTTCAAGCT+TGG	+	chr5_4:40440480-40440499	Msa0850720:CDS	45.0%
	AGCAAGGGATTTCAAGCTTG+GGG	+	chr5_4:40440482-40440501	Msa0850720:CDS	45.0%
	TGTGCCTCTTCATTGCTATC+TGG	+	chr5_4:40440585-40440604	Msa0850720:CDS	45.0%
!!	TTGCTTTGGTGCTCTCACTT+TGG	+	chr5_4:40440692-40440711	Msa0850720:CDS	45.0%
!!	TGCTTTGGTGCTCTCACTTT+GGG	+	chr5_4:40440693-40440712	Msa0850720:CDS	45.0%
!!	CTAGCTTTGTACCCAACTTG+AGG	+	chr5_4:40440876-40440895	Msa0850720:three_prime_UTR	45.0%
!!!	ATTTTTTTAATTCTTTATTT+AGG	+	chr5_4:40440115-40440134	Msa0850720:intron	5.0%
!!!	ATTTTTTTAATTCTTTATTT+AGG	+	chr5_4:40440115-40440134	Msa0850720:intron	5.0%
!	AACGGTAACGGTAACGGTTG+TGG	-	chr5_4:40439044-40439063	None:intergenic	50.0%
	GCCGAAGCGATAAACAAAGG+TGG	-	chr5_4:40439117-40439136	None:intergenic	50.0%
!	CTCACACTTAGCTTTCACCG+CGG	+	chr5_4:40439227-40439246	Msa0850720:CDS	50.0%
!!!	GACCCTTGGGAGTGTTGTTT+TGG	+	chr5_4:40439314-40439333	Msa0850720:CDS	50.0%
	GTGCCAAAACAACACTCCCA+AGG	-	chr5_4:40439320-40439339	None:intergenic	50.0%
	TTGAGAAACAGAGAGAGGGG+GGG	-	chr5_4:40439930-40439949	None:intergenic	50.0%
	AGGTGATTCCAAGGGAAGCA+AGG	+	chr5_4:40440466-40440485	Msa0850720:intron	50.0%
	GGTGATTCCAAGGGAAGCAA+GGG	+	chr5_4:40440467-40440486	Msa0850720:intron	50.0%
	AGCAGAGAAAGGGGTTGCTT+TGG	+	chr5_4:40440678-40440697	Msa0850720:CDS	50.0%
!!	GCTTTGGTGCTCTCACTTTG+GGG	+	chr5_4:40440694-40440713	Msa0850720:CDS	50.0%
!	AACGGTAACGGTAACGGTTG+TGG	-	chr5_4:40439044-40439063	None:intergenic	50.0%
	GCCGAAGCGATAAACAAAGG+TGG	-	chr5_4:40439117-40439136	None:intergenic	50.0%
!	CTCACACTTAGCTTTCACCG+CGG	+	chr5_4:40439227-40439246	Msa0850720:CDS	50.0%
!!!	GACCCTTGGGAGTGTTGTTT+TGG	+	chr5_4:40439314-40439333	Msa0850720:CDS	50.0%
	GTGCCAAAACAACACTCCCA+AGG	-	chr5_4:40439320-40439339	None:intergenic	50.0%
	TTGAGAAACAGAGAGAGGGG+GGG	-	chr5_4:40439930-40439949	None:intergenic	50.0%
	AGGTGATTCCAAGGGAAGCA+AGG	+	chr5_4:40440466-40440485	Msa0850720:intron	50.0%
	GGTGATTCCAAGGGAAGCAA+GGG	+	chr5_4:40440467-40440486	Msa0850720:intron	50.0%
	AGCAGAGAAAGGGGTTGCTT+TGG	+	chr5_4:40440678-40440697	Msa0850720:CDS	50.0%
!!	GCTTTGGTGCTCTCACTTTG+GGG	+	chr5_4:40440694-40440713	Msa0850720:CDS	50.0%
	GGGTCCACCACATGTTCCTA+TGG	-	chr5_4:40438911-40438930	None:intergenic	55.0%
	TCCATGGCGGCAAACGTGTA+TGG	+	chr5_4:40438953-40438972	Msa0850720:CDS	55.0%
	GCAGTCATCGGAGTTCTCAC+CGG	+	chr5_4:40439135-40439154	Msa0850720:CDS	55.0%
!	CTGCGGCATAAAGCGTCTAC+CGG	+	chr5_4:40439179-40439198	Msa0850720:CDS	55.0%
	CGCGGTGAAAGCTAAGTGTG+AGG	-	chr5_4:40439229-40439248	None:intergenic	55.0%
	TGAGAAACAGAGAGAGGGGG+GGG	-	chr5_4:40439929-40439948	None:intergenic	55.0%
	GGGTCCACCACATGTTCCTA+TGG	-	chr5_4:40438911-40438930	None:intergenic	55.0%
	TCCATGGCGGCAAACGTGTA+TGG	+	chr5_4:40438953-40438972	Msa0850720:CDS	55.0%
	GCAGTCATCGGAGTTCTCAC+CGG	+	chr5_4:40439135-40439154	Msa0850720:CDS	55.0%
!	CTGCGGCATAAAGCGTCTAC+CGG	+	chr5_4:40439179-40439198	Msa0850720:CDS	55.0%
	CGCGGTGAAAGCTAAGTGTG+AGG	-	chr5_4:40439229-40439248	None:intergenic	55.0%
	TGAGAAACAGAGAGAGGGGG+GGG	-	chr5_4:40439929-40439948	None:intergenic	55.0%
	GCCATACACGTTTGCCGCCA+TGG	-	chr5_4:40438957-40438976	None:intergenic	60.0%
	GACGACTACCTCTACGCCTG+CGG	+	chr5_4:40439162-40439181	Msa0850720:CDS	60.0%
	GCTTTATGCCGCAGGCGTAG+AGG	-	chr5_4:40439173-40439192	None:intergenic	60.0%
	CGGTAGACGCTTTATGCCGC+AGG	-	chr5_4:40439181-40439200	None:intergenic	60.0%
	AGAAACAGAGAGAGGGGGGG+GGG	-	chr5_4:40439927-40439946	None:intergenic	60.0%
	GAGAAACAGAGAGAGGGGGG+GGG	-	chr5_4:40439928-40439947	None:intergenic	60.0%
	GCCATACACGTTTGCCGCCA+TGG	-	chr5_4:40438957-40438976	None:intergenic	60.0%
	GACGACTACCTCTACGCCTG+CGG	+	chr5_4:40439162-40439181	Msa0850720:CDS	60.0%
	GCTTTATGCCGCAGGCGTAG+AGG	-	chr5_4:40439173-40439192	None:intergenic	60.0%
	CGGTAGACGCTTTATGCCGC+AGG	-	chr5_4:40439181-40439200	None:intergenic	60.0%
	AGAAACAGAGAGAGGGGGGG+GGG	-	chr5_4:40439927-40439946	None:intergenic	60.0%
	GAGAAACAGAGAGAGGGGGG+GGG	-	chr5_4:40439928-40439947	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr5_4	gene	40438706	40441001	40438706	ID=Msa0850720;Name=Msa0850720
chr5_4	mRNA	40438706	40441001	40438706	ID=Msa0850720-mRNA-1;Parent=Msa0850720;Name=Msa0850720-mRNA-1;_AED=0.00;_eAED=0.00;_QI=114\|1\|1\|1\|1\|1\|2\|264\|342
chr5_4	exon	40438706	40439578	40438706	ID=Msa0850720-mRNA-1:exon:12748;Parent=Msa0850720-mRNA-1
chr5_4	exon	40440468	40441001	40440468	ID=Msa0850720-mRNA-1:exon:12749;Parent=Msa0850720-mRNA-1
chr5_4	five_prime_UTR	40438706	40438819	40438706	ID=Msa0850720-mRNA-1:five_prime_utr;Parent=Msa0850720-mRNA-1
chr5_4	CDS	40438820	40439578	40438820	ID=Msa0850720-mRNA-1:cds;Parent=Msa0850720-mRNA-1
chr5_4	CDS	40440468	40440737	40440468	ID=Msa0850720-mRNA-1:cds;Parent=Msa0850720-mRNA-1
chr5_4	three_prime_UTR	40440738	40441001	40440738	ID=Msa0850720-mRNA-1:three_prime_utr;Parent=Msa0850720-mRNA-1

Gene Sequence

>Msa0850720

ATGGTGGAAAGAATAATTAACGAAGAAGAAGAAAGCAAAAAAAGAAGCATGAAGAAACTCCTTCTAATACTAAACTCAATCCTCTTAGCCATAGGAACATGTGGTGGACCCCTAGTAATGCGTCTATACTTCATCCATGGCGGCAAACGTGTATGGCTCTCAAGCTTCCTTGAAACATCTGCCTTCCCAATAATTCTCATTCCCTTAGCCATTTCTCACATCCACAACCGTTACCGTTACCGTTACGAAAACCCTAACGGCAACAACAATAATAACAACTTTGTCTCAATGAAACCACCTTTGTTTATCGCTTCGGCAGTCATCGGAGTTCTCACCGGTTTAGACGACTACCTCTACGCCTGCGGCATAAAGCGTCTACCGGTGTCAACTTCTTCGTTAATTCAATCCTCACACTTAGCTTTCACCGCGGTTTTCGCGTTTTTTCTTGTGAAGCATAAGTTCACAGCTTATTCTGTTAACTCTATTGTTTTGTTGACCCTTGGGAGTGTTGTTTTGGCACTGAATTCTGATGGTGGAGATAGACTTGTTGGTGAGTCTACTAAGGATTATGTGATTGGTTTTGTTATGATATTGGCTTCTGCTGCTTTGTATGGATTTGTTTTACCGTTGTTGGAGTTGGTTTATAAAAAGAGTAAACAAGCTATTACTTATTCTCTTGTTATGGAGATTCAGCTTGTTATGTGCTTCTTTGCTACTTTGTTTTGCGTTGTTGGGATGATCATAGACAATGATTTTAAGGTGATTCCAAGGGAAGCAAGGGATTTCAAGCTTGGGGAAACAAAGTACTATGTTGTGCTAGTGTGGAGTGCTATAATGTGGCAATTTTTCTTCTTAGGAGCGATTGGAGTTATCTTTTGTGCCTCTTCATTGCTATCTGGTATCATAATTGCTGCATTTCTTCCCATAACTGAAATCTTAGCTGTTATTGTCTACAAAGAGAAATTTTCAAGCAGAGAAAGGGGTTGCTTTGGTGCTCTCACTTTGGGGCTTTGTGTCTTACTTTTATGA

Protein sequence

>Msa0850720

MVERIINEEEESKKRSMKKLLLILNSILLAIGTCGGPLVMRLYFIHGGKRVWLSSFLETSAFPIILIPLAISHIHNRYRYRYENPNGNNNNNNFVSMKPPLFIASAVIGVLTGLDDYLYACGIKRLPVSTSSLIQSSHLAFTAVFAFFLVKHKFTAYSVNSIVLLTLGSVVLALNSDGGDRLVGESTKDYVIGFVMILASAALYGFVLPLLELVYKKSKQAITYSLVMEIQLVMCFFATLFCVVGMIIDNDFKVIPREARDFKLGETKYYVVLVWSAIMWQFFFLGAIGVIFCASSLLSGIIIAAFLPITEILAVIVYKEKFSSRERGCFGALTLGLCVLLL*