AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1020540


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1020540 MtrunA17_Chr7g0220411 77.632 152 4 1 1 122 53 204 2.41e-79 232
Msa1020540 MtrunA17_Chr7g0220401 53.247 77 28 6 42 113 56 129 4.41e-12 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 29 sgRNAs with CRISPR-Local

Find 131 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTATACACTATGGATTATT+AGG 0.191132 7_1:-44185555 None:intergenic
TTGTGATGTTGTGCCAATTA+AGG 0.246710 7_1:+44185386 Msa1020540:CDS
AGAGAGACTTCAAAGATTTC+AGG 0.285228 7_1:+44184496 Msa1020540:CDS
TGTTTCTTCATGATGCATTA+TGG 0.299984 7_1:-44184449 None:intergenic
CTCTCTGGGCTACCCTTAAT+TGG 0.306444 7_1:-44185399 None:intergenic
ATAGACCTTGTTGATCTCTC+TGG 0.319933 7_1:-44185414 None:intergenic
ATTAGGTTGGTGGTTATTGA+TGG 0.351867 7_1:-44185538 None:intergenic
GGTTATTGATGGGTGGCCTA+TGG 0.383756 7_1:-44185527 None:intergenic
CATATGAGGAAGCTAGGATT+AGG 0.383881 7_1:+44184424 Msa1020540:CDS
TGTGATGTTGTGCCAATTAA+GGG 0.423992 7_1:+44185387 Msa1020540:CDS
GTTATTGATGGGTGGCCTAT+GGG 0.426738 7_1:-44185526 None:intergenic
ACAAGTGATGATTCTAAGAA+TGG 0.430722 7_1:+44184469 Msa1020540:CDS
TACACTATGGATTATTAGGT+TGG 0.431300 7_1:-44185551 None:intergenic
ATTGATGCTGCAAAAGAGAT+TGG 0.439380 7_1:+44185462 Msa1020540:CDS
TATAAAGACTATAGTCATAA+GGG 0.449557 7_1:+44185495 Msa1020540:CDS
GTATAAAGACTATAGTCATA+AGG 0.458134 7_1:+44185494 Msa1020540:CDS
TTAGGTTGGTGGTTATTGAT+GGG 0.477398 7_1:-44185537 None:intergenic
AATCCAGTGATTCTCTGAAA+AGG 0.485827 7_1:-44184397 None:intergenic
TAGACCTTGTTGATCTCTCT+GGG 0.503078 7_1:-44185413 None:intergenic
ATTCCTTGGAATTTCAAACT+TGG 0.505171 7_1:-44185328 None:intergenic
TCACCAAGTTTGAAATTCCA+AGG 0.527456 7_1:+44185325 Msa1020540:CDS
ACTATGGATTATTAGGTTGG+TGG 0.530182 7_1:-44185548 None:intergenic
TGAACACTTTGTGTTTGTGT+TGG 0.535270 7_1:-44185358 None:intergenic
TTGATGGGTGGCCTATGGGA+TGG 0.541064 7_1:-44185522 None:intergenic
GGTTGGTGGTTATTGATGGG+TGG 0.541114 7_1:-44185534 None:intergenic
GTGTTGGTGAGGCTATTCCT+TGG 0.562537 7_1:-44185342 None:intergenic
ACTTTGTGTTTGTGTTGGTG+AGG 0.582251 7_1:-44185353 None:intergenic
ATAAGGGAAAGCCATCCCAT+AGG 0.646277 7_1:+44185511 Msa1020540:CDS
GTAGCCCAGAGAGATCAACA+AGG 0.801014 7_1:+44185409 Msa1020540:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTTGTTCATTTTTAAAT+CGG + chr7_1:44185187-44185206 Msa1020540:intron 10.0%
!! TGTTTAATAGAATATTAATA+TGG - chr7_1:44185267-44185286 None:intergenic 10.0%
!!! AAATTTGTTCATTTTTAAAT+CGG + chr7_1:44185187-44185206 Msa1020540:intron 10.0%
!! TGTTTAATAGAATATTAATA+TGG - chr7_1:44185267-44185286 None:intergenic 10.0%
!! ACAATATACTAGAATATTAA+TGG - chr7_1:44184843-44184862 None:intergenic 15.0%
!! ACAATATACTAGAATATTAA+TGG - chr7_1:44184843-44184862 None:intergenic 15.0%
!!! TAATTTTCTAGCTTATGAAA+AGG + chr7_1:44184816-44184835 Msa1020540:intron 20.0%
!! AATATTCTAGTATATTGTGA+AGG + chr7_1:44184845-44184864 Msa1020540:intron 20.0%
!! ACAGAAAATTATATATTGCA+AGG - chr7_1:44185100-44185119 None:intergenic 20.0%
!! TAAAAAGTTGTATATTCGTA+AGG - chr7_1:44185130-44185149 None:intergenic 20.0%
!!! AACTTTTTACTTTTGATCTT+CGG + chr7_1:44185141-44185160 Msa1020540:intron 20.0%
!!! AAAAATGAACAAATTTTGCA+GGG - chr7_1:44185183-44185202 None:intergenic 20.0%
!!! TAAAAATGAACAAATTTTGC+AGG - chr7_1:44185184-44185203 None:intergenic 20.0%
!! TATAAAGACTATAGTCATAA+GGG + chr7_1:44185495-44185514 Msa1020540:CDS 20.0%
!!! TAATTTTCTAGCTTATGAAA+AGG + chr7_1:44184816-44184835 Msa1020540:intron 20.0%
!! AATATTCTAGTATATTGTGA+AGG + chr7_1:44184845-44184864 Msa1020540:intron 20.0%
!! ACAGAAAATTATATATTGCA+AGG - chr7_1:44185100-44185119 None:intergenic 20.0%
!! TAAAAAGTTGTATATTCGTA+AGG - chr7_1:44185130-44185149 None:intergenic 20.0%
!!! AACTTTTTACTTTTGATCTT+CGG + chr7_1:44185141-44185160 Msa1020540:intron 20.0%
!!! AAAAATGAACAAATTTTGCA+GGG - chr7_1:44185183-44185202 None:intergenic 20.0%
!!! TAAAAATGAACAAATTTTGC+AGG - chr7_1:44185184-44185203 None:intergenic 20.0%
!! TATAAAGACTATAGTCATAA+GGG + chr7_1:44185495-44185514 Msa1020540:CDS 20.0%
! TATAAAGAATACGCATGTTA+AGG - chr7_1:44184898-44184917 None:intergenic 25.0%
! GTATAAAGACTATAGTCATA+AGG + chr7_1:44185494-44185513 Msa1020540:CDS 25.0%
! TATAAAGAATACGCATGTTA+AGG - chr7_1:44184898-44184917 None:intergenic 25.0%
! GTATAAAGACTATAGTCATA+AGG + chr7_1:44185494-44185513 Msa1020540:CDS 25.0%
!!! TCACTGGATTTTTTCATATG+AGG + chr7_1:44184410-44184429 Msa1020540:CDS 30.0%
!!! TTTTTTCATATGAGGAAGCT+AGG + chr7_1:44184418-44184437 Msa1020540:CDS 30.0%
TGTTTCTTCATGATGCATTA+TGG - chr7_1:44184452-44184471 None:intergenic 30.0%
! ACAAGTGATGATTCTAAGAA+TGG + chr7_1:44184469-44184488 Msa1020540:CDS 30.0%
ATGGAATATGTGACTTTGAA+AGG + chr7_1:44184533-44184552 Msa1020540:intron 30.0%
! TGGAATATGTGACTTTGAAA+GGG + chr7_1:44184534-44184553 Msa1020540:intron 30.0%
GATTTCACTGATGATTATCA+TGG + chr7_1:44184646-44184665 Msa1020540:intron 30.0%
! TTTCACACTCAGTTATGTTT+TGG - chr7_1:44184688-44184707 None:intergenic 30.0%
ATTCCTTGGAATTTCAAACT+TGG - chr7_1:44185331-44185350 None:intergenic 30.0%
!!! TCACTGGATTTTTTCATATG+AGG + chr7_1:44184410-44184429 Msa1020540:CDS 30.0%
!!! TTTTTTCATATGAGGAAGCT+AGG + chr7_1:44184418-44184437 Msa1020540:CDS 30.0%
TGTTTCTTCATGATGCATTA+TGG - chr7_1:44184452-44184471 None:intergenic 30.0%
! ACAAGTGATGATTCTAAGAA+TGG + chr7_1:44184469-44184488 Msa1020540:CDS 30.0%
ATGGAATATGTGACTTTGAA+AGG + chr7_1:44184533-44184552 Msa1020540:intron 30.0%
! TGGAATATGTGACTTTGAAA+GGG + chr7_1:44184534-44184553 Msa1020540:intron 30.0%
GATTTCACTGATGATTATCA+TGG + chr7_1:44184646-44184665 Msa1020540:intron 30.0%
! TTTCACACTCAGTTATGTTT+TGG - chr7_1:44184688-44184707 None:intergenic 30.0%
ATTCCTTGGAATTTCAAACT+TGG - chr7_1:44185331-44185350 None:intergenic 30.0%
! AATCCAGTGATTCTCTGAAA+AGG - chr7_1:44184400-44184419 None:intergenic 35.0%
AGAGAGACTTCAAAGATTTC+AGG + chr7_1:44184496-44184515 Msa1020540:CDS 35.0%
! AATATGTGACTTTGAAAGGG+AGG + chr7_1:44184537-44184556 Msa1020540:intron 35.0%
! ATATGTGACTTTGAAAGGGA+GGG + chr7_1:44184538-44184557 Msa1020540:intron 35.0%
ACCAACAAGAAAGCTGAAAA+TGG + chr7_1:44184609-44184628 Msa1020540:intron 35.0%
! TCCATTTTCAGCTTTCTTGT+TGG - chr7_1:44184613-44184632 None:intergenic 35.0%
! AGAAAGCTGAAAATGGACTT+TGG + chr7_1:44184616-44184635 Msa1020540:intron 35.0%
!! ACACTCAGTTATGTTTTGGA+AGG - chr7_1:44184684-44184703 None:intergenic 35.0%
! ACAGAAGCATATGACAATGA+AGG + chr7_1:44185284-44185303 Msa1020540:intron 35.0%
! TCACCAAGTTTGAAATTCCA+AGG + chr7_1:44185325-44185344 Msa1020540:CDS 35.0%
TGAACACTTTGTGTTTGTGT+TGG - chr7_1:44185361-44185380 None:intergenic 35.0%
TTGTGATGTTGTGCCAATTA+AGG + chr7_1:44185386-44185405 Msa1020540:CDS 35.0%
TGTGATGTTGTGCCAATTAA+GGG + chr7_1:44185387-44185406 Msa1020540:CDS 35.0%
ATTGATGCTGCAAAAGAGAT+TGG + chr7_1:44185462-44185481 Msa1020540:CDS 35.0%
!! TTAGGTTGGTGGTTATTGAT+GGG - chr7_1:44185540-44185559 None:intergenic 35.0%
!! ATTAGGTTGGTGGTTATTGA+TGG - chr7_1:44185541-44185560 None:intergenic 35.0%
! AATCCAGTGATTCTCTGAAA+AGG - chr7_1:44184400-44184419 None:intergenic 35.0%
AGAGAGACTTCAAAGATTTC+AGG + chr7_1:44184496-44184515 Msa1020540:CDS 35.0%
! AATATGTGACTTTGAAAGGG+AGG + chr7_1:44184537-44184556 Msa1020540:intron 35.0%
! ATATGTGACTTTGAAAGGGA+GGG + chr7_1:44184538-44184557 Msa1020540:intron 35.0%
ACCAACAAGAAAGCTGAAAA+TGG + chr7_1:44184609-44184628 Msa1020540:intron 35.0%
! TCCATTTTCAGCTTTCTTGT+TGG - chr7_1:44184613-44184632 None:intergenic 35.0%
! AGAAAGCTGAAAATGGACTT+TGG + chr7_1:44184616-44184635 Msa1020540:intron 35.0%
!! ACACTCAGTTATGTTTTGGA+AGG - chr7_1:44184684-44184703 None:intergenic 35.0%
! ACAGAAGCATATGACAATGA+AGG + chr7_1:44185284-44185303 Msa1020540:intron 35.0%
! TCACCAAGTTTGAAATTCCA+AGG + chr7_1:44185325-44185344 Msa1020540:CDS 35.0%
TGAACACTTTGTGTTTGTGT+TGG - chr7_1:44185361-44185380 None:intergenic 35.0%
TTGTGATGTTGTGCCAATTA+AGG + chr7_1:44185386-44185405 Msa1020540:CDS 35.0%
TGTGATGTTGTGCCAATTAA+GGG + chr7_1:44185387-44185406 Msa1020540:CDS 35.0%
ATTGATGCTGCAAAAGAGAT+TGG + chr7_1:44185462-44185481 Msa1020540:CDS 35.0%
!! TTAGGTTGGTGGTTATTGAT+GGG - chr7_1:44185540-44185559 None:intergenic 35.0%
!! ATTAGGTTGGTGGTTATTGA+TGG - chr7_1:44185541-44185560 None:intergenic 35.0%
! GAACCTTTTCAGAGAATCAC+TGG + chr7_1:44184394-44184413 Msa1020540:CDS 40.0%
CATATGAGGAAGCTAGGATT+AGG + chr7_1:44184424-44184443 Msa1020540:CDS 40.0%
TCAGGTGCAACTTATTGTGA+TGG + chr7_1:44184514-44184533 Msa1020540:intron 40.0%
! TATGTGACTTTGAAAGGGAG+GGG + chr7_1:44184539-44184558 Msa1020540:intron 40.0%
CTGATGATTATCATGGACCA+AGG + chr7_1:44184653-44184672 Msa1020540:intron 40.0%
!!! CTCAGTTATGTTTTGGAAGG+TGG - chr7_1:44184681-44184700 None:intergenic 40.0%
AGCTATAGCTTCATCAGTGT+AGG + chr7_1:44184740-44184759 Msa1020540:intron 40.0%
CATTCTCATTACCTGTGGAA+TGG - chr7_1:44184988-44185007 None:intergenic 40.0%
TCCACAGGTAATGAGAATGA+TGG + chr7_1:44184989-44185008 Msa1020540:intron 40.0%
ACCATCATTCTCATTACCTG+TGG - chr7_1:44184993-44185012 None:intergenic 40.0%
GTAATGAGAATGATGGTCTG+AGG + chr7_1:44184996-44185015 Msa1020540:intron 40.0%
GCATATGACAATGAAGGCAA+AGG + chr7_1:44185290-44185309 Msa1020540:intron 40.0%
! ACTTTGTGTTTGTGTTGGTG+AGG - chr7_1:44185356-44185375 None:intergenic 40.0%
TAGACCTTGTTGATCTCTCT+GGG - chr7_1:44185416-44185435 None:intergenic 40.0%
ATAGACCTTGTTGATCTCTC+TGG - chr7_1:44185417-44185436 None:intergenic 40.0%
CCACCAACCTAATAATCCAT+AGG + chr7_1:44185548-44185567 Msa1020540:CDS 40.0%
! GAACCTTTTCAGAGAATCAC+TGG + chr7_1:44184394-44184413 Msa1020540:CDS 40.0%
CATATGAGGAAGCTAGGATT+AGG + chr7_1:44184424-44184443 Msa1020540:CDS 40.0%
TCAGGTGCAACTTATTGTGA+TGG + chr7_1:44184514-44184533 Msa1020540:intron 40.0%
! TATGTGACTTTGAAAGGGAG+GGG + chr7_1:44184539-44184558 Msa1020540:intron 40.0%
CTGATGATTATCATGGACCA+AGG + chr7_1:44184653-44184672 Msa1020540:intron 40.0%
!!! CTCAGTTATGTTTTGGAAGG+TGG - chr7_1:44184681-44184700 None:intergenic 40.0%
AGCTATAGCTTCATCAGTGT+AGG + chr7_1:44184740-44184759 Msa1020540:intron 40.0%
CATTCTCATTACCTGTGGAA+TGG - chr7_1:44184988-44185007 None:intergenic 40.0%
TCCACAGGTAATGAGAATGA+TGG + chr7_1:44184989-44185008 Msa1020540:intron 40.0%
ACCATCATTCTCATTACCTG+TGG - chr7_1:44184993-44185012 None:intergenic 40.0%
GTAATGAGAATGATGGTCTG+AGG + chr7_1:44184996-44185015 Msa1020540:intron 40.0%
GCATATGACAATGAAGGCAA+AGG + chr7_1:44185290-44185309 Msa1020540:intron 40.0%
! ACTTTGTGTTTGTGTTGGTG+AGG - chr7_1:44185356-44185375 None:intergenic 40.0%
TAGACCTTGTTGATCTCTCT+GGG - chr7_1:44185416-44185435 None:intergenic 40.0%
ATAGACCTTGTTGATCTCTC+TGG - chr7_1:44185417-44185436 None:intergenic 40.0%
! CTATGTTGCTTCCATTCCAC+AGG + chr7_1:44184974-44184993 Msa1020540:intron 45.0%
ATAAGGGAAAGCCATCCCAT+AGG + chr7_1:44185511-44185530 Msa1020540:CDS 45.0%
! GTTATTGATGGGTGGCCTAT+GGG - chr7_1:44185529-44185548 None:intergenic 45.0%
! CTATGTTGCTTCCATTCCAC+AGG + chr7_1:44184974-44184993 Msa1020540:intron 45.0%
ATAAGGGAAAGCCATCCCAT+AGG + chr7_1:44185511-44185530 Msa1020540:CDS 45.0%
! GTTATTGATGGGTGGCCTAT+GGG - chr7_1:44185529-44185548 None:intergenic 45.0%
!!! TGTTTTGGAAGGTGGTGCCT+TGG - chr7_1:44184673-44184692 None:intergenic 50.0%
GCGCATCGTTCGTTTGTACT+TGG - chr7_1:44185042-44185061 None:intergenic 50.0%
AGTACAAACGAACGATGCGC+AGG + chr7_1:44185042-44185061 Msa1020540:intron 50.0%
! GTGTTGGTGAGGCTATTCCT+TGG - chr7_1:44185345-44185364 None:intergenic 50.0%
CTCTCTGGGCTACCCTTAAT+TGG - chr7_1:44185402-44185421 None:intergenic 50.0%
GTAGCCCAGAGAGATCAACA+AGG + chr7_1:44185409-44185428 Msa1020540:CDS 50.0%
! GGTTATTGATGGGTGGCCTA+TGG - chr7_1:44185530-44185549 None:intergenic 50.0%
!! GGTTGGTGGTTATTGATGGG+TGG - chr7_1:44185537-44185556 None:intergenic 50.0%
!!! TGTTTTGGAAGGTGGTGCCT+TGG - chr7_1:44184673-44184692 None:intergenic 50.0%
GCGCATCGTTCGTTTGTACT+TGG - chr7_1:44185042-44185061 None:intergenic 50.0%
AGTACAAACGAACGATGCGC+AGG + chr7_1:44185042-44185061 Msa1020540:intron 50.0%
! GTGTTGGTGAGGCTATTCCT+TGG - chr7_1:44185345-44185364 None:intergenic 50.0%
CTCTCTGGGCTACCCTTAAT+TGG - chr7_1:44185402-44185421 None:intergenic 50.0%
GTAGCCCAGAGAGATCAACA+AGG + chr7_1:44185409-44185428 Msa1020540:CDS 50.0%
! GGTTATTGATGGGTGGCCTA+TGG - chr7_1:44185530-44185549 None:intergenic 50.0%
!! GGTTGGTGGTTATTGATGGG+TGG - chr7_1:44185537-44185556 None:intergenic 50.0%
! TTGATGGGTGGCCTATGGGA+TGG - chr7_1:44185525-44185544 None:intergenic 55.0%
! TTGATGGGTGGCCTATGGGA+TGG - chr7_1:44185525-44185544 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr7_1 gene 44184394 44185569 44184394 ID=Msa1020540;Name=Msa1020540
chr7_1 mRNA 44184394 44185569 44184394 ID=Msa1020540-mRNA-1;Parent=Msa1020540;Name=Msa1020540-mRNA-1;_AED=0.36;_eAED=0.36;_QI=0|0|0|1|0|0.5|2|0|122
chr7_1 exon 44184394 44184517 44184394 ID=Msa1020540-mRNA-1:exon:8552;Parent=Msa1020540-mRNA-1
chr7_1 exon 44185325 44185569 44185325 ID=Msa1020540-mRNA-1:exon:8553;Parent=Msa1020540-mRNA-1
chr7_1 CDS 44184394 44184517 44184394 ID=Msa1020540-mRNA-1:cds;Parent=Msa1020540-mRNA-1
chr7_1 CDS 44185325 44185569 44185325 ID=Msa1020540-mRNA-1:cds;Parent=Msa1020540-mRNA-1
Gene Sequence

>Msa1020540

GAACCTTTTCAGAGAATCACTGGATTTTTTCATATGAGGAAGCTAGGATTAGGAACCATAATGCATCATGAAGAAACAAGTGATGATTCTAAGAATGGAAAAAGAGAGACTTCAAAGATTTCAGTCACCAAGTTTGAAATTCCAAGGAATAGCCTCACCAACACAAACACAAAGTGTTCACAAAATTGTGATGTTGTGCCAATTAAGGGTAGCCCAGAGAGATCAACAAGGTCTATACAAATTTCTGAAGAAGCTCAAAGTATTGATGCTGCAAAAGAGATTGGAAGTCTCATGTATAAAGACTATAGTCATAAGGGAAAGCCATCCCATAGGCCACCCATCAATAACCACCAACCTAATAATCCATAG

Protein sequence

>Msa1020540

EPFQRITGFFHMRKLGLGTIMHHEETSDDSKNGKRETSKISVTKFEIPRNSLTNTNTKCSQNCDVVPIKGSPERSTRSIQISEEAQSIDAAKEIGSLMYKDYSHKGKPSHRPPINNHQPNNP*