AlfalfaGEDB | Msa0143740

M. sativa cultivar zhongmu-4 / Msa0143740

KEGG: -
GO: -
PFAM: PF13637.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0143740	XP_024640501.2	66.424	688	130	7	1	589	28	713	0.0	882

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0143740	A0A396HTM3	66.383	705	120	10	1	590	32	734	0.0	871

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0010150	Msa0143740	0.813484	2.500739e-51	-8.615850e-47
Msa0048950	Msa0143740	0.848029	7.949778e-60	-8.615850e-47
Msa0051100	Msa0143740	-0.800140	1.657117e-48	-8.615850e-47
Msa0054760	Msa0143740	-0.800277	1.554391e-48	-8.615850e-47
Msa0059880	Msa0143740	0.881825	1.739553e-70	-8.615850e-47
Msa0072030	Msa0143740	0.811547	6.629839e-51	-8.615850e-47
Msa0101650	Msa0143740	-0.803765	2.981274e-49	-8.615850e-47
Msa0139460	Msa0143740	0.812300	4.544892e-51	-8.615850e-47
Msa0140270	Msa0143740	0.809521	1.815891e-50	-8.615850e-47
Msa0143680	Msa0143740	0.964465	2.318490e-123	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0143740	MtrunA17_Chr5g0410171	66.383	705	120	10	1	590	32	734	0.0	871
Msa0143740	MtrunA17_Chr5g0410341	88.358	335	6	2	288	589	411	745	0.0	585
Msa0143740	MtrunA17_Chr5g0410341	87.859	313	14	4	1	289	30	342	0.0	555
Msa0143740	MtrunA17_Chr5g0410201	77.744	328	40	2	296	590	1	328	1.25e-178	508
Msa0143740	MtrunA17_Chr5g0410271	77.538	325	40	2	298	589	2	326	3.09e-175	499
Msa0143740	MtrunA17_Chr6g0457101	41.333	675	291	16	1	588	30	686	7.67e-149	446
Msa0143740	MtrunA17_Chr5g0410461	52.660	376	135	7	260	594	96	469	6.48e-113	346
Msa0143740	MtrunA17_Chr6g0456141	52.035	344	124	6	288	597	51	387	2.79e-111	338
Msa0143740	MtrunA17_Chr6g0456101	36.262	717	311	24	1	583	30	734	1.35e-108	343
Msa0143740	MtrunA17_Chr5g0410371	59.122	296	82	4	329	587	1	294	1.72e-102	313

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0143740	AT3G18670.1	35.751	579	281	19	70	587	35	583	4.60e-98	311
Msa0143740	AT3G18670.2	33.654	624	288	19	70	587	35	638	2.00e-95	306
Msa0143740	AT5G04690.3	30.756	582	312	14	81	587	66	631	2.69e-66	229
Msa0143740	AT5G04690.1	31.616	563	312	16	81	587	66	611	1.08e-64	224
Msa0143740	AT5G04690.2	30.201	596	311	15	81	587	66	645	5.92e-64	223
Msa0143740	AT5G04700.1	29.711	589	310	15	90	587	80	655	4.99e-61	215
Msa0143740	AT5G04680.3	28.767	584	316	17	90	587	67	636	1.09e-53	194
Msa0143740	AT5G04680.4	28.523	596	316	17	90	587	67	650	5.24e-52	190
Msa0143740	AT5G04730.1	29.795	537	284	16	138	587	58	588	4.34e-50	183
Msa0143740	AT5G04680.2	28.571	532	274	14	151	587	7	527	2.09e-48	177

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Find 144 sgRNAs with CRISPR-Local

Find 306 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAAGCCTGGAATAAATT+GGG	0.481257	1_4:+2784399	Msa0143740:CDS
AAATGCCGTGTAGACGCATT+TGG	0.419337	1_4:+2787750	Msa0143740:CDS
AAATGGGCAAAAGATACAGC+TGG	0.580445	1_4:+2788465	Msa0143740:CDS
AACAGGGATTTCACCATCAT+TGG	0.379563	1_4:-2785645	None:intergenic
AACCCATATCCACTACCACT+TGG	0.557493	1_4:-2786000	None:intergenic
AACTACCGCAACGTGAAGAA+CGG	0.612033	1_4:-2785432	None:intergenic
AAGAACGGTCCTCCCCATAG+AGG	0.622685	1_4:-2785417	None:intergenic
AAGAGAAATTCTATGGTTCA+AGG	0.541072	1_4:+2787854	Msa0143740:CDS
AAGTGTGATTTGTGGGCAAG+AGG	0.558162	1_4:-2785922	None:intergenic
AATATATTCCACATCCTCTA+TGG	0.260429	1_4:+2785403	Msa0143740:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CTAAGACTGAATATGAGATT+TGG	+	chr1_4:2784289-2784308	Msa0143740:CDS	30.0%
CATATCATTCAACTAACATG+TGG	+	chr1_4:2784336-2784355	Msa0143740:CDS	30.0%
CTAATATGAGTAAGATTCTC+TGG	-	chr1_4:2784363-2784382	None:intergenic	30.0%
TAAAGAAGCCTGGAATAAAT+TGG	+	chr1_4:2784398-2784417	Msa0143740:CDS	30.0%
AAAGAAGCCTGGAATAAATT+GGG	+	chr1_4:2784399-2784418	Msa0143740:CDS	30.0%
ATAAATAAAGTGTGTGAGTG+AGG	+	chr1_4:2784795-2784814	Msa0143740:intron	30.0%
GTTGTATGAGATGTTACAAA+TGG	+	chr1_4:2784874-2784893	Msa0143740:intron	30.0%
TAGTTACAACGCTTAAGATT+GGG	-	chr1_4:2785072-2785091	None:intergenic	30.0%
TTAGTTACAACGCTTAAGAT+TGG	-	chr1_4:2785073-2785092	None:intergenic	30.0%
TTGTAACTAAGAACAACCAA+CGG	+	chr1_4:2785083-2785102	Msa0143740:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_4	gene	2784225	2788986	2784225	ID=Msa0143740;Name=Msa0143740
chr1_4	mRNA	2784225	2788986	2784225	ID=Msa0143740-mRNA-1;Parent=Msa0143740;Name=Msa0143740-mRNA-1;_AED=0.08;_eAED=0.08;_QI=0\|0\|0\|1\|0.16\|0.14\|7\|0\|610
chr1_4	exon	2784225	2784468	2784225	ID=Msa0143740-mRNA-1:exon:1250;Parent=Msa0143740-mRNA-1
chr1_4	exon	2785300	2785530	2785300	ID=Msa0143740-mRNA-1:exon:1251;Parent=Msa0143740-mRNA-1
chr1_4	exon	2785540	2785761	2785540	ID=Msa0143740-mRNA-1:exon:1252;Parent=Msa0143740-mRNA-1
chr1_4	exon	2785884	2786045	2785884	ID=Msa0143740-mRNA-1:exon:1253;Parent=Msa0143740-mRNA-1
chr1_4	exon	2787439	2787687	2787439	ID=Msa0143740-mRNA-1:exon:1254;Parent=Msa0143740-mRNA-1
chr1_4	exon	2787697	2787875	2787697	ID=Msa0143740-mRNA-1:exon:1255;Parent=Msa0143740-mRNA-1
chr1_4	exon	2788444	2788986	2788444	ID=Msa0143740-mRNA-1:exon:1256;Parent=Msa0143740-mRNA-1
chr1_4	CDS	2784225	2784468	2784225	ID=Msa0143740-mRNA-1:cds;Parent=Msa0143740-mRNA-1
chr1_4	CDS	2785300	2785530	2785300	ID=Msa0143740-mRNA-1:cds;Parent=Msa0143740-mRNA-1
chr1_4	CDS	2785540	2785761	2785540	ID=Msa0143740-mRNA-1:cds;Parent=Msa0143740-mRNA-1
chr1_4	CDS	2785884	2786045	2785884	ID=Msa0143740-mRNA-1:cds;Parent=Msa0143740-mRNA-1
chr1_4	CDS	2787439	2787687	2787439	ID=Msa0143740-mRNA-1:cds;Parent=Msa0143740-mRNA-1
chr1_4	CDS	2787697	2787875	2787697	ID=Msa0143740-mRNA-1:cds;Parent=Msa0143740-mRNA-1
chr1_4	CDS	2788444	2788986	2788444	ID=Msa0143740-mRNA-1:cds;Parent=Msa0143740-mRNA-1

Gene Sequence

>Msa0143740

AACTATCTTGTGGGACATGGTCTGTGGGGTGTTGTGACCTCTATTCCTGAAATTGGTGCGCGTTCTAAGACTGAATATGAGATTTGGAACAGGAAGAATGGGAAGGCATTGCATATCATTCAACTAACATGTGGACCAGAGAATCTTACTCATATTAGAGATCTTCATATTGCTAAAGAAGCCTGGAATAAATTGGGTGCAACTTATAGCTCAGATTTGCAAGCCGATCCAGATATTGAGCAAGGTGTGGTGGATGATAGTCTCCTTCAATATAAATCGCTGCACAAGTATATTGAGAGCGGTGAGTGGAAAGATGCAAACTCGTTTATTAAGAGGGACAGTACAGCAATATATTCCACATCCTCTATGGGGAGGACCGTTCTTCACGTTGCGGTAGTTGCTGGGCACGAGGAAATTGTGAAGAAATTGGTGAAGGAAGGGAAAGATAAGTTAGTGAAAATGAAAGACAATCGAGCCCTAGCTCTTGTTGCTGAGCTGACCGGAAACACGAATATAGCGAAGTGTATGACTATGGTATATAAGAAAATATCACGGTCAGAGGACCTGTTATCTATGAAGACCAATGATGGTGAAATCCCTGTTCTTCTTGCTGCTGCTAAGGGACACAAAGAAATGACACGTTATCTTTACCGTTGTACACCGTTGGAAGACCTGGCCGATGATAATTATCGCAATGGTGTGGCTTTGAGTTTACTCCAACGATTTCCTCAAATGCCTCTTGCCCACAAATCACACTTTGAATCGGATTGTGTGCAACCATTATACGCATTGGCACGCATGCCTTCTGTGTTTCCAAGTGGTAGTGGATATGGGTTCATACGACAATTCTTTAACGATAGAATAAGGAAAATATATAATCAGAAAATGACCTACCGTGTAGCTCTTGAAATATTGAGTTGCTTGCACCAAAGAATTCAAGAGTTTAAGGAATCAGAACTTAGAGAGGCTTCGGCTTACGATGCAATGTTGCAAGCAGCAAAGCATGGGATTATTGAGTTTATAGATGCAATGAGAAAGGGTAATCCTGACCTCTTATGGGCCATTGATAAAAATAAAAGAGGCGTATTTTCACATGCAATTTTGAACCCCGTTTTCGAACTCATACATGATGCAACTGTAAATGGACGGAAGGAGATAGTTAAATGCCGTGTAGACGCATTTGGCAATTCCATGTTGCACTTGGCAGGATATTTAGGGCCTTCCTCTGATCTTGATCGTAGATCCGGTCCTGCTATGCAGATGCAAAGAGAAATTCTATGGTTCAAGGAGCTGGTGAAAGCTGGGGAGAAATGGGCAAAAGATACAGCTGGTTCATTTACTCTTGTGGCTACACTCATCACTACTATCATGTTTGCTGCAGCTTTCACTGTCCCTGGTGGAAACAATCAAGACAATGGAGTTCCCTTGTTCTTGCACGACATCACATTTGATGTGTTTATTATAGCAGATGCAGCATCTCTTTTCACTTCTTCAACTTCGGTCCTTCTCTTCATTGGAATCCTAACGGCTCGTTACGCTGAAAAAGATTTCCTTAAGTCATTGCCACTAAGGTTACTTTTCGCACTCATTGCCCTTTTCTTCTCTGTGGTTTCTATGATAGTTGCCTTCTGTGCTTCACTTGCTATGCTCTTAAAGGGACATCATAGAGTCATAATAACAGCAATGTCATTTGCCAGTGTTCCTGTTATCGTTCTAGTTCCGTCACAATTGCGCCTCTTCCTTGAGATTTTCAAGTCTACAGTATTATATCCAATTGAAACTTATTACTTATCTAGAACGAACTGTAACATTTGTTTTTGCATTGATTGC

Protein sequence

>Msa0143740

NYLVGHGLWGVVTSIPEIGARSKTEYEIWNRKNGKALHIIQLTCGPENLTHIRDLHIAKEAWNKLGATYSSDLQADPDIEQGVVDDSLLQYKSLHKYIESGEWKDANSFIKRDSTAIYSTSSMGRTVLHVAVVAGHEEIVKKLVKEGKDKLVKMKDNRALALVAELTGNTNIAKCMTMVYKKISRSEDLLSMKTNDGEIPVLLAAAKGHKEMTRYLYRCTPLEDLADDNYRNGVALSLLQRFPQMPLAHKSHFESDCVQPLYALARMPSVFPSGSGYGFIRQFFNDRIRKIYNQKMTYRVALEILSCLHQRIQEFKESELREASAYDAMLQAAKHGIIEFIDAMRKGNPDLLWAIDKNKRGVFSHAILNPVFELIHDATVNGRKEIVKCRVDAFGNSMLHLAGYLGPSSDLDRRSGPAMQMQREILWFKELVKAGEKWAKDTAGSFTLVATLITTIMFAAAFTVPGGNNQDNGVPLFLHDITFDVFIIADAASLFTSSTSVLLFIGILTARYAEKDFLKSLPLRLLFALIALFFSVVSMIVAFCASLAMLLKGHHRVIITAMSFASVPVIVLVPSQLRLFLEIFKSTVLYPIETYYLSRTNCNICFCIDC