Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0235140 | XP_003593755.1 | 70.159 | 315 | 90 | 3 | 1 | 314 | 1 | 312 | 7.98e-144 | 418 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0235140 | sp|Q9FVS1|FBK23_ARATH | 30.000 | 200 | 128 | 6 | 6 | 197 | 2 | 197 | 1.16e-15 | 80.1 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0235140 | G7ILH6 | 70.159 | 315 | 90 | 3 | 1 | 314 | 1 | 312 | 3.81e-144 | 418 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0007770 | Msa0235140 | 0.806202 | 9.225111e-50 | -8.615850e-47 |
| Msa0016570 | Msa0235140 | 0.825222 | 5.274151e-54 | -8.615850e-47 |
| Msa0056530 | Msa0235140 | 0.800848 | 1.188712e-48 | -8.615850e-47 |
| Msa0102070 | Msa0235140 | 0.819976 | 8.751227e-53 | -8.615850e-47 |
| Msa0111510 | Msa0235140 | 0.828031 | 1.127126e-54 | -8.615850e-47 |
| Msa0123810 | Msa0235140 | 0.822211 | 2.674165e-53 | -8.615850e-47 |
| Msa0142220 | Msa0235140 | 0.830765 | 2.442656e-55 | -8.615850e-47 |
| Msa0145750 | Msa0235140 | 0.827866 | 1.234599e-54 | -8.615850e-47 |
| Msa0153910 | Msa0235140 | 0.840871 | 6.697002e-58 | -8.615850e-47 |
| Msa0159720 | Msa0235140 | 0.811556 | 6.598183e-51 | -8.615850e-47 |
| Msa0165190 | Msa0235140 | 0.815721 | 7.994056e-52 | -8.615850e-47 |
| Msa0188460 | Msa0235140 | 0.824328 | 8.566536e-54 | -8.615850e-47 |
| Msa0192970 | Msa0235140 | 0.845688 | 3.474159e-59 | -8.615850e-47 |
| Msa0227480 | Msa0235140 | 0.824403 | 8.225685e-54 | -8.615850e-47 |
| Msa0231000 | Msa0235140 | 0.817343 | 3.464444e-52 | -8.615850e-47 |
| Msa0232850 | Msa0235140 | 0.807730 | 4.381623e-50 | -8.615850e-47 |
| Msa0235140 | Msa0267000 | 0.818397 | 2.002033e-52 | -8.615850e-47 |
| Msa0235140 | Msa0267010 | 0.817313 | 3.518759e-52 | -8.615850e-47 |
| Msa0235140 | Msa0270520 | 0.843321 | 1.505210e-58 | -8.615850e-47 |
| Msa0235140 | Msa0273100 | 0.801045 | 1.083721e-48 | -8.615850e-47 |
| Msa0235140 | Msa0306460 | 0.802160 | 6.399388e-49 | -8.615850e-47 |
| Msa0235140 | Msa0309020 | 0.825205 | 5.323255e-54 | -8.615850e-47 |
| Msa0235140 | Msa0313760 | 0.940600 | 1.767397e-100 | -8.615850e-47 |
| Msa0235140 | Msa0337900 | 0.811917 | 5.507913e-51 | -8.615850e-47 |
| Msa0235140 | Msa0342240 | 0.825286 | 5.090929e-54 | -8.615850e-47 |
| Msa0235140 | Msa0355220 | 0.804573 | 2.024924e-49 | -8.615850e-47 |
| Msa0235140 | Msa0360510 | 0.831428 | 1.678345e-55 | -8.615850e-47 |
| Msa0235140 | Msa0363300 | 0.803666 | 3.126951e-49 | -8.615850e-47 |
| Msa0235140 | Msa0384800 | 0.882816 | 7.583229e-71 | -8.615850e-47 |
| Msa0235140 | Msa0390070 | 0.810677 | 1.023258e-50 | -8.615850e-47 |
| Msa0235140 | Msa0402290 | 0.811645 | 6.312363e-51 | -8.615850e-47 |
| Msa0235140 | Msa0406120 | 0.850424 | 1.712990e-60 | -8.615850e-47 |
| Msa0235140 | Msa0414410 | 0.837190 | 6.014651e-57 | -8.615850e-47 |
| Msa0235140 | Msa0414420 | 0.854714 | 1.023719e-61 | -8.615850e-47 |
| Msa0235140 | Msa0435230 | 0.804197 | 2.424763e-49 | -8.615850e-47 |
| Msa0235140 | Msa0436780 | 0.806044 | 9.956762e-50 | -8.615850e-47 |
| Msa0235140 | Msa0436800 | 0.824268 | 8.851602e-54 | -8.615850e-47 |
| Msa0235140 | Msa0438620 | 0.804794 | 1.820274e-49 | -8.615850e-47 |
| Msa0235140 | Msa0450000 | 0.815558 | 8.692963e-52 | -8.615850e-47 |
| Msa0235140 | Msa0452900 | 0.834144 | 3.551614e-56 | -8.615850e-47 |
| Msa0235140 | Msa0453120 | 0.809173 | 2.157233e-50 | -8.615850e-47 |
| Msa0235140 | Msa0454910 | 0.808235 | 3.421443e-50 | -8.615850e-47 |
| Msa0235140 | Msa0459160 | 0.829781 | 4.247000e-55 | -8.615850e-47 |
| Msa0235140 | Msa0459170 | 0.835682 | 1.455931e-56 | -8.615850e-47 |
| Msa0235140 | Msa0506270 | 0.803430 | 3.500110e-49 | -8.615850e-47 |
| Msa0235140 | Msa0564890 | 0.870341 | 1.570630e-66 | -8.615850e-47 |
| Msa0235140 | Msa0589780 | 0.810748 | 9.877628e-51 | -8.615850e-47 |
| Msa0235140 | Msa0596150 | 0.802516 | 5.405285e-49 | -8.615850e-47 |
| Msa0235140 | Msa0598780 | 0.818446 | 1.952143e-52 | -8.615850e-47 |
| Msa0235140 | Msa0639610 | 0.809884 | 1.517086e-50 | -8.615850e-47 |
| Msa0235140 | Msa0668260 | 0.810775 | 9.747164e-51 | -8.615850e-47 |
| Msa0235140 | Msa0678930 | 0.817284 | 3.570942e-52 | -8.615850e-47 |
| Msa0235140 | Msa0694370 | 0.816280 | 5.998866e-52 | -8.615850e-47 |
| Msa0235140 | Msa0697060 | 0.820905 | 5.358260e-53 | -8.615850e-47 |
| Msa0235140 | Msa0726140 | 0.878815 | 2.069696e-69 | -8.615850e-47 |
| Msa0235140 | Msa0735080 | 0.807924 | 3.985338e-50 | -8.615850e-47 |
| Msa0235140 | Msa0736000 | 0.815778 | 7.766459e-52 | -8.615850e-47 |
| Msa0235140 | Msa0743810 | 0.852947 | 3.304154e-61 | -8.615850e-47 |
| Msa0235140 | Msa0745060 | 0.816079 | 6.652328e-52 | -8.615850e-47 |
| Msa0235140 | Msa0766630 | 0.820599 | 6.298637e-53 | -8.615850e-47 |
| Msa0235140 | Msa0767720 | 0.832815 | 7.621475e-56 | -8.615850e-47 |
| Msa0235140 | Msa0783160 | 0.823286 | 1.503366e-53 | -8.615850e-47 |
| Msa0235140 | Msa0787600 | 0.819408 | 1.179785e-52 | -8.615850e-47 |
| Msa0235140 | Msa0811080 | 0.825762 | 3.927278e-54 | -8.615850e-47 |
| Msa0235140 | Msa0815140 | 0.801594 | 8.363253e-49 | -8.615850e-47 |
| Msa0235140 | Msa0825860 | 0.825197 | 5.345619e-54 | -8.615850e-47 |
| Msa0235140 | Msa0833290 | 0.815015 | 1.147701e-51 | -8.615850e-47 |
| Msa0235140 | Msa0846330 | 0.814101 | 1.828733e-51 | -8.615850e-47 |
| Msa0235140 | Msa0846350 | 0.838829 | 2.278669e-57 | -8.615850e-47 |
| Msa0235140 | Msa0865320 | 0.803864 | 2.843573e-49 | -8.615850e-47 |
| Msa0235140 | Msa0885280 | 0.849385 | 3.345850e-60 | -8.615850e-47 |
| Msa0235140 | Msa0885290 | 0.852385 | 4.780480e-61 | -8.615850e-47 |
| Msa0235140 | Msa0887020 | 0.801783 | 7.650432e-49 | -8.615850e-47 |
| Msa0235140 | Msa0907260 | 0.818959 | 1.493004e-52 | -8.615850e-47 |
| Msa0235140 | Msa0922720 | 0.802335 | 5.889884e-49 | -8.615850e-47 |
| Msa0235140 | Msa0935910 | 0.808263 | 3.375742e-50 | -8.615850e-47 |
| Msa0235140 | Msa0954910 | 0.804495 | 2.101478e-49 | -8.615850e-47 |
| Msa0235140 | Msa0956270 | 0.811723 | 6.070784e-51 | -8.615850e-47 |
| Msa0235140 | Msa0981370 | 0.827797 | 1.282578e-54 | -8.615850e-47 |
| Msa0235140 | Msa0989060 | 0.859994 | 2.812435e-63 | -8.615850e-47 |
| Msa0235140 | Msa0998690 | 0.818816 | 1.609191e-52 | -8.615850e-47 |
| Msa0235140 | Msa1002310 | 0.802334 | 5.894936e-49 | -8.615850e-47 |
| Msa0235140 | Msa1016280 | 0.821280 | 4.391281e-53 | -8.615850e-47 |
| Msa0235140 | Msa1024240 | 0.813159 | 2.947110e-51 | -8.615850e-47 |
| Msa0235140 | Msa1025360 | 0.826456 | 2.685512e-54 | -8.615850e-47 |
| Msa0235140 | Msa1025370 | 0.817439 | 3.295918e-52 | -8.615850e-47 |
| Msa0235140 | Msa1044830 | 0.822842 | 1.908106e-53 | -8.615850e-47 |
| Msa0235140 | Msa1044840 | 0.828935 | 6.815705e-55 | -8.615850e-47 |
| Msa0235140 | Msa1092990 | 0.815382 | 9.512386e-52 | -8.615850e-47 |
| Msa0235140 | Msa1100980 | 0.822209 | 2.677439e-53 | -8.615850e-47 |
| Msa0235140 | Msa1109440 | 0.846673 | 1.873362e-59 | -8.615850e-47 |
| Msa0235140 | Msa1114030 | 0.802228 | 6.197212e-49 | -8.615850e-47 |
| Msa0235140 | Msa1121200 | 0.817983 | 2.484163e-52 | -8.615850e-47 |
| Msa0235140 | Msa1135740 | 0.817415 | 3.336354e-52 | -8.615850e-47 |
| Msa0235140 | Msa1136090 | 0.855393 | 6.501481e-62 | -8.615850e-47 |
| Msa0235140 | Msa1150370 | 0.816974 | 4.194017e-52 | -8.615850e-47 |
| Msa0235140 | Msa1160670 | 0.837097 | 6.354986e-57 | -8.615850e-47 |
| Msa0235140 | Msa1187940 | 0.825455 | 4.643735e-54 | -8.615850e-47 |
| Msa0235140 | Msa1187950 | 0.823467 | 1.363717e-53 | -8.615850e-47 |
| Msa0235140 | Msa1192640 | 0.848022 | 7.986363e-60 | -8.615850e-47 |
| Msa0235140 | Msa1260100 | 0.801509 | 8.707619e-49 | -8.615850e-47 |
| Msa0235140 | Msa1269670 | 0.815692 | 8.115388e-52 | -8.615850e-47 |
| Msa0235140 | Msa1270230 | 0.819351 | 1.215575e-52 | -8.615850e-47 |
| Msa0235140 | Msa1270240 | 0.818442 | 1.955937e-52 | -8.615850e-47 |
| Msa0235140 | Msa1283330 | 0.841325 | 5.089922e-58 | -8.615850e-47 |
| Msa0235140 | Msa1285930 | 0.866516 | 2.693149e-65 | -8.615850e-47 |
| Msa0235140 | Msa1287800 | 0.844172 | 8.909886e-59 | -8.615850e-47 |
| Msa0235140 | Msa1287810 | 0.849638 | 2.843368e-60 | -8.615850e-47 |
| Msa0235140 | Msa1287820 | 0.827364 | 1.629493e-54 | -8.615850e-47 |
| Msa0235140 | Msa1287830 | 0.837013 | 6.676781e-57 | -8.615850e-47 |
| Msa0235140 | Msa1289430 | 0.811510 | 6.751788e-51 | -8.615850e-47 |
| Msa0235140 | Msa1297210 | 0.807118 | 5.908203e-50 | -8.615850e-47 |
| Msa0235140 | Msa1307890 | 0.801275 | 9.725335e-49 | -8.615850e-47 |
| Msa0235140 | Msa1310120 | 0.801210 | 1.002744e-48 | -8.615850e-47 |
| Msa0235140 | Msa1369430 | 0.828120 | 1.072459e-54 | -8.615850e-47 |
| Msa0235140 | Msa1404000 | 0.849377 | 3.362871e-60 | -8.615850e-47 |
| Msa0235140 | Msa1422180 | 0.843920 | 1.041294e-58 | -8.615850e-47 |
| Msa0235140 | Msa1433120 | 0.825167 | 5.431887e-54 | -8.615850e-47 |
| Msa0235140 | Msa1438250 | 0.801535 | 8.602414e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0235140 | MtrunA17_Chr2g0283611 | 70.159 | 315 | 90 | 3 | 1 | 314 | 1 | 312 | 7.34e-148 | 418 |
| Msa0235140 | MtrunA17_Chr2g0283601 | 89.130 | 46 | 5 | 0 | 1 | 46 | 1 | 46 | 1.32e-21 | 87.0 |
| Msa0235140 | MtrunA17_Chr2g0283601 | 75.556 | 45 | 11 | 0 | 270 | 314 | 42 | 86 | 1.71e-17 | 76.3 |
| Msa0235140 | MtrunA17_Chr1g0178491 | 33.742 | 163 | 99 | 4 | 17 | 176 | 36 | 192 | 1.63e-21 | 93.6 |
| Msa0235140 | MtrunA17_Chr7g0266441 | 28.402 | 169 | 106 | 4 | 17 | 176 | 32 | 194 | 1.23e-13 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0235140 | AT1G57790.1 | 30.000 | 200 | 128 | 6 | 6 | 197 | 2 | 197 | 1.18e-16 | 80.1 |
| Msa0235140 | AT4G10660.1 | 30.909 | 165 | 97 | 6 | 82 | 237 | 38 | 194 | 3.51e-16 | 78.2 |
| Msa0235140 | AT4G10695.2 | 25.217 | 230 | 152 | 8 | 43 | 262 | 15 | 234 | 1.04e-15 | 75.5 |
| Msa0235140 | AT4G10695.1 | 25.217 | 230 | 152 | 8 | 43 | 262 | 15 | 234 | 1.04e-15 | 75.5 |
| Msa0235140 | AT1G49360.5 | 32.468 | 231 | 129 | 12 | 9 | 222 | 77 | 297 | 1.94e-15 | 77.0 |
| Msa0235140 | AT1G49360.1 | 32.468 | 231 | 129 | 12 | 9 | 222 | 100 | 320 | 2.12e-15 | 77.0 |
| Msa0235140 | AT1G49360.3 | 32.727 | 220 | 121 | 12 | 20 | 222 | 8 | 217 | 3.62e-15 | 75.9 |
| Msa0235140 | AT1G49360.2 | 32.727 | 220 | 121 | 12 | 20 | 222 | 8 | 217 | 3.62e-15 | 75.9 |
| Msa0235140 | AT1G49360.4 | 32.727 | 220 | 121 | 12 | 20 | 222 | 8 | 217 | 3.62e-15 | 75.9 |
| Msa0235140 | AT3G18720.1 | 28.520 | 277 | 173 | 15 | 13 | 271 | 44 | 313 | 3.69e-15 | 75.9 |
| Msa0235140 | AT4G12370.1 | 44.444 | 90 | 44 | 4 | 83 | 170 | 18 | 103 | 2.88e-13 | 69.7 |
| Msa0235140 | AT4G00893.1 | 31.111 | 180 | 107 | 6 | 10 | 181 | 38 | 208 | 6.33e-13 | 69.3 |
| Msa0235140 | AT4G12382.1 | 32.558 | 129 | 75 | 4 | 8 | 130 | 1 | 123 | 3.68e-11 | 60.5 |
| Msa0235140 | AT4G12382.2 | 32.558 | 129 | 75 | 4 | 8 | 130 | 1 | 123 | 3.68e-11 | 60.5 |
Find 64 sgRNAs with CRISPR-Local
Find 140 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGATGATCATCTCCTTAA+AGG | 0.136143 | 2_2:+7973484 | Msa0235140:CDS |
| GCACGATCCATACTCCTTTA+AGG | 0.163291 | 2_2:-7973498 | None:intergenic |
| TATATTCATGGTGGTAAAAT+TGG | 0.170543 | 2_2:-7973132 | None:intergenic |
| ATAATTTGTTTCTCTTTAAA+AGG | 0.183643 | 2_2:+7973152 | Msa0235140:CDS |
| ATGGAGCGAGTGTAGTTTAT+TGG | 0.249967 | 2_2:+7973255 | Msa0235140:CDS |
| ACTCTATGATGCTCAAAATT+TGG | 0.258194 | 2_2:+7973429 | Msa0235140:CDS |
| CAAGTTGACCCAGCCAGTTC+TGG | 0.303744 | 2_2:-7972866 | None:intergenic |
| AGAGAAACAAATTATATTCA+TGG | 0.329456 | 2_2:-7973144 | None:intergenic |
| GATTCTGTGATAGGACAATT+TGG | 0.336565 | 2_2:-7972977 | None:intergenic |
| ATCATCTCCTTAAAGGAGTA+TGG | 0.340645 | 2_2:+7973491 | Msa0235140:CDS |
| TCTTAGTGTTTATGATATCT+TGG | 0.361386 | 2_2:-7973520 | None:intergenic |
| TGCTGTCAAATATTGCAAAT+AGG | 0.363050 | 2_2:+7972666 | Msa0235140:CDS |
| GCGCTCTGCGACTGAACCTT+TGG | 0.370991 | 2_2:+7972736 | Msa0235140:CDS |
| TTGGTTGACGCTAAATTTAG+TGG | 0.396967 | 2_2:+7972755 | Msa0235140:CDS |
| TTCATCTGCTCCCACGTCTC+AGG | 0.396997 | 2_2:+7973021 | Msa0235140:CDS |
| CAGTCATTCGATTCTGTGAT+AGG | 0.398376 | 2_2:-7972986 | None:intergenic |
| ACTACACTCGCTCCATTGTT+TGG | 0.401720 | 2_2:-7973248 | None:intergenic |
| TTCATCATGTGTGCAAAGAT+TGG | 0.403215 | 2_2:+7972714 | Msa0235140:CDS |
| TATAACTACAAGTTTATTGC+TGG | 0.411591 | 2_2:-7973209 | None:intergenic |
| TGAAAGGAAAACTAGGACTT+GGG | 0.413009 | 2_2:+7973377 | Msa0235140:CDS |
| TTTGTCGGGGAGATAATTCA+TGG | 0.421163 | 2_2:+7973101 | Msa0235140:CDS |
| AGAACTGCACACGCTTTGTC+GGG | 0.421785 | 2_2:+7973087 | Msa0235140:CDS |
| CACTATCGAAATTCCAGAAC+TGG | 0.428141 | 2_2:+7972853 | Msa0235140:CDS |
| CTGAAAGGAAAACTAGGACT+TGG | 0.430022 | 2_2:+7973376 | Msa0235140:CDS |
| TGGTGTGGGGTGATTACATT+TGG | 0.434879 | 2_2:+7973449 | Msa0235140:CDS |
| GATCCATGGTTTCTGTTGTA+TGG | 0.439332 | 2_2:+7972782 | Msa0235140:CDS |
| GTCAAATATTGCAAATAGGC+TGG | 0.448250 | 2_2:+7972670 | Msa0235140:CDS |
| AGGTGTGCATTCTTTATTGA+AGG | 0.450535 | 2_2:+7973172 | Msa0235140:CDS |
| TAGAACTGCACACGCTTTGT+CGG | 0.462441 | 2_2:+7973086 | Msa0235140:CDS |
| GCCTTGCATCCTACGACGGA+TGG | 0.482267 | 2_2:+7972888 | Msa0235140:CDS |
| TTTATCATCCTTGTTTAGCA+TGG | 0.488027 | 2_2:-7972825 | None:intergenic |
| ATCGAAATTCCAGAACTGGC+TGG | 0.492699 | 2_2:+7972857 | Msa0235140:CDS |
| ATATTTGACAGCAGATCATA+AGG | 0.498174 | 2_2:-7972656 | None:intergenic |
| AGTATTGAAATGTAGATTGA+GGG | 0.511372 | 2_2:+7973306 | Msa0235140:CDS |
| CAGTTCTAATTTATATTGAG+TGG | 0.524994 | 2_2:-7973071 | None:intergenic |
| GGTGTGCATTCTTTATTGAA+GGG | 0.534190 | 2_2:+7973173 | Msa0235140:CDS |
| CAGTATTGAAATGTAGATTG+AGG | 0.535602 | 2_2:+7973305 | Msa0235140:CDS |
| ATGTTGCAATAGATACTTGT+GGG | 0.537656 | 2_2:+7973404 | Msa0235140:CDS |
| AAGGATCTGAAAGGAAAACT+AGG | 0.543003 | 2_2:+7973370 | Msa0235140:CDS |
| AATTTAGTGGATGTGATCCA+TGG | 0.549686 | 2_2:+7972768 | Msa0235140:CDS |
| TCGAAATTCCAGAACTGGCT+GGG | 0.550468 | 2_2:+7972858 | Msa0235140:CDS |
| GTGTGCATTCTTTATTGAAG+GGG | 0.552789 | 2_2:+7973174 | Msa0235140:CDS |
| GCCATCCGTCGTAGGATGCA+AGG | 0.563825 | 2_2:-7972889 | None:intergenic |
| TGATGCTCAAAATTTGGTGT+GGG | 0.569401 | 2_2:+7973435 | Msa0235140:CDS |
| TTTCTGTTGTATGGTGAAGA+AGG | 0.575330 | 2_2:+7972791 | Msa0235140:CDS |
| AAAGGGAAAGCATCGATCAA+AGG | 0.578404 | 2_2:+7973351 | Msa0235140:CDS |
| ATGATGCTCAAAATTTGGTG+TGG | 0.583531 | 2_2:+7973434 | Msa0235140:CDS |
| CTAAATTTAGCGTCAACCAA+AGG | 0.595406 | 2_2:-7972752 | None:intergenic |
| GAGAAGCAGCCATCCGTCGT+AGG | 0.605702 | 2_2:-7972897 | None:intergenic |
| ACAATGCAATCCTGAGACGT+GGG | 0.606280 | 2_2:-7973031 | None:intergenic |
| AACAATGCAATCCTGAGACG+TGG | 0.609541 | 2_2:-7973032 | None:intergenic |
| TCGTTGTTCCATGCTAAACA+AGG | 0.611298 | 2_2:+7972817 | Msa0235140:CDS |
| TTGAGTGGCGTGATTAATCA+CGG | 0.617271 | 2_2:-7973056 | None:intergenic |
| TCACCATACAACAGAAACCA+TGG | 0.617900 | 2_2:-7972785 | None:intergenic |
| CATCGATCAAAGGATCTGAA+AGG | 0.623852 | 2_2:+7973361 | Msa0235140:CDS |
| AAAAGAAAAGCAAAGGAGAG+TGG | 0.624016 | 2_2:+7972624 | Msa0235140:CDS |
| ACTTGCCTTGCATCCTACGA+CGG | 0.635214 | 2_2:+7972884 | Msa0235140:CDS |
| GAACTGCACACGCTTTGTCG+GGG | 0.640853 | 2_2:+7973088 | Msa0235140:CDS |
| GAAAGGAAAACTAGGACTTG+GGG | 0.666358 | 2_2:+7973378 | Msa0235140:CDS |
| AATGTTGCAATAGATACTTG+TGG | 0.675405 | 2_2:+7973403 | Msa0235140:CDS |
| GAAACAAATTATATTCATGG+TGG | 0.683671 | 2_2:-7973141 | None:intergenic |
| ACGTTGCTAATACCAAACAA+TGG | 0.689270 | 2_2:+7973236 | Msa0235140:CDS |
| GATGCTCAAAATTTGGTGTG+GGG | 0.729523 | 2_2:+7973436 | Msa0235140:CDS |
| TTGTTTAGCATGGAACAACG+AGG | 0.763244 | 2_2:-7972815 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATAATTTGTTTCTCTTTAAA+AGG | + | chr2_2:7973152-7973171 | Msa0235140:CDS | 15.0% |
| !!! | ATAATTTGTTTCTCTTTAAA+AGG | + | chr2_2:7973152-7973171 | Msa0235140:CDS | 15.0% |
| !! | AGAGAAACAAATTATATTCA+TGG | - | chr2_2:7973147-7973166 | None:intergenic | 20.0% |
| !! | AGAGAAACAAATTATATTCA+TGG | - | chr2_2:7973147-7973166 | None:intergenic | 20.0% |
| !!! | TTTGCTTTTCTTTTTGCATA+TGG | - | chr2_2:7972618-7972637 | None:intergenic | 25.0% |
| ! | ATATGCAAAAAGAAAAGCAA+AGG | + | chr2_2:7972617-7972636 | Msa0235140:CDS | 25.0% |
| !!! | TGTATTTTTTCTCTAGAGAA+GGG | - | chr2_2:7972953-7972972 | None:intergenic | 25.0% |
| !!! | ATGTATTTTTTCTCTAGAGA+AGG | - | chr2_2:7972954-7972973 | None:intergenic | 25.0% |
| !! | CAGTTCTAATTTATATTGAG+TGG | - | chr2_2:7973074-7973093 | None:intergenic | 25.0% |
| ! | TATATTCATGGTGGTAAAAT+TGG | - | chr2_2:7973135-7973154 | None:intergenic | 25.0% |
| ! | GAAACAAATTATATTCATGG+TGG | - | chr2_2:7973144-7973163 | None:intergenic | 25.0% |
| ! | TATAACTACAAGTTTATTGC+TGG | - | chr2_2:7973212-7973231 | None:intergenic | 25.0% |
| !! | AGTATTGAAATGTAGATTGA+GGG | + | chr2_2:7973306-7973325 | Msa0235140:CDS | 25.0% |
| ! | ATTGTCAAAGAAAGCAAAAA+AGG | + | chr2_2:7973333-7973352 | Msa0235140:CDS | 25.0% |
| ! | TTGTCAAAGAAAGCAAAAAA+GGG | + | chr2_2:7973334-7973353 | Msa0235140:CDS | 25.0% |
| !!! | TTTGCTTTTCTTTTTGCATA+TGG | - | chr2_2:7972618-7972637 | None:intergenic | 25.0% |
| ! | ATATGCAAAAAGAAAAGCAA+AGG | + | chr2_2:7972617-7972636 | Msa0235140:CDS | 25.0% |
| !!! | TGTATTTTTTCTCTAGAGAA+GGG | - | chr2_2:7972953-7972972 | None:intergenic | 25.0% |
| !!! | ATGTATTTTTTCTCTAGAGA+AGG | - | chr2_2:7972954-7972973 | None:intergenic | 25.0% |
| !! | CAGTTCTAATTTATATTGAG+TGG | - | chr2_2:7973074-7973093 | None:intergenic | 25.0% |
| ! | TATATTCATGGTGGTAAAAT+TGG | - | chr2_2:7973135-7973154 | None:intergenic | 25.0% |
| ! | GAAACAAATTATATTCATGG+TGG | - | chr2_2:7973144-7973163 | None:intergenic | 25.0% |
| ! | TATAACTACAAGTTTATTGC+TGG | - | chr2_2:7973212-7973231 | None:intergenic | 25.0% |
| !! | AGTATTGAAATGTAGATTGA+GGG | + | chr2_2:7973306-7973325 | Msa0235140:CDS | 25.0% |
| ! | ATTGTCAAAGAAAGCAAAAA+AGG | + | chr2_2:7973333-7973352 | Msa0235140:CDS | 25.0% |
| ! | TTGTCAAAGAAAGCAAAAAA+GGG | + | chr2_2:7973334-7973353 | Msa0235140:CDS | 25.0% |
| ATATTTGACAGCAGATCATA+AGG | - | chr2_2:7972659-7972678 | None:intergenic | 30.0% | |
| TGCTGTCAAATATTGCAAAT+AGG | + | chr2_2:7972666-7972685 | Msa0235140:CDS | 30.0% | |
| ! | TTTATCATCCTTGTTTAGCA+TGG | - | chr2_2:7972828-7972847 | None:intergenic | 30.0% |
| !!! | GTATTTTTTCTCTAGAGAAG+GGG | - | chr2_2:7972952-7972971 | None:intergenic | 30.0% |
| ! | CAGTATTGAAATGTAGATTG+AGG | + | chr2_2:7973305-7973324 | Msa0235140:CDS | 30.0% |
| AATGTTGCAATAGATACTTG+TGG | + | chr2_2:7973403-7973422 | Msa0235140:CDS | 30.0% | |
| ATGTTGCAATAGATACTTGT+GGG | + | chr2_2:7973404-7973423 | Msa0235140:CDS | 30.0% | |
| ACTCTATGATGCTCAAAATT+TGG | + | chr2_2:7973429-7973448 | Msa0235140:CDS | 30.0% | |
| ! | TTTGATGATCATCTCCTTAA+AGG | + | chr2_2:7973484-7973503 | Msa0235140:CDS | 30.0% |
| ATATTTGACAGCAGATCATA+AGG | - | chr2_2:7972659-7972678 | None:intergenic | 30.0% | |
| TGCTGTCAAATATTGCAAAT+AGG | + | chr2_2:7972666-7972685 | Msa0235140:CDS | 30.0% | |
| ! | TTTATCATCCTTGTTTAGCA+TGG | - | chr2_2:7972828-7972847 | None:intergenic | 30.0% |
| !!! | GTATTTTTTCTCTAGAGAAG+GGG | - | chr2_2:7972952-7972971 | None:intergenic | 30.0% |
| ! | CAGTATTGAAATGTAGATTG+AGG | + | chr2_2:7973305-7973324 | Msa0235140:CDS | 30.0% |
| AATGTTGCAATAGATACTTG+TGG | + | chr2_2:7973403-7973422 | Msa0235140:CDS | 30.0% | |
| ATGTTGCAATAGATACTTGT+GGG | + | chr2_2:7973404-7973423 | Msa0235140:CDS | 30.0% | |
| ACTCTATGATGCTCAAAATT+TGG | + | chr2_2:7973429-7973448 | Msa0235140:CDS | 30.0% | |
| ! | TTTGATGATCATCTCCTTAA+AGG | + | chr2_2:7973484-7973503 | Msa0235140:CDS | 30.0% |
| AAAAGAAAAGCAAAGGAGAG+TGG | + | chr2_2:7972624-7972643 | Msa0235140:CDS | 35.0% | |
| GTCAAATATTGCAAATAGGC+TGG | + | chr2_2:7972670-7972689 | Msa0235140:CDS | 35.0% | |
| TTCATCATGTGTGCAAAGAT+TGG | + | chr2_2:7972714-7972733 | Msa0235140:CDS | 35.0% | |
| CTAAATTTAGCGTCAACCAA+AGG | - | chr2_2:7972755-7972774 | None:intergenic | 35.0% | |
| TTGGTTGACGCTAAATTTAG+TGG | + | chr2_2:7972755-7972774 | Msa0235140:CDS | 35.0% | |
| ! | AATTTAGTGGATGTGATCCA+TGG | + | chr2_2:7972768-7972787 | Msa0235140:CDS | 35.0% |
| TTTCTGTTGTATGGTGAAGA+AGG | + | chr2_2:7972791-7972810 | Msa0235140:CDS | 35.0% | |
| GATTCTGTGATAGGACAATT+TGG | - | chr2_2:7972980-7972999 | None:intergenic | 35.0% | |
| AGGTGTGCATTCTTTATTGA+AGG | + | chr2_2:7973172-7973191 | Msa0235140:CDS | 35.0% | |
| GGTGTGCATTCTTTATTGAA+GGG | + | chr2_2:7973173-7973192 | Msa0235140:CDS | 35.0% | |
| GTGTGCATTCTTTATTGAAG+GGG | + | chr2_2:7973174-7973193 | Msa0235140:CDS | 35.0% | |
| ACGTTGCTAATACCAAACAA+TGG | + | chr2_2:7973236-7973255 | Msa0235140:CDS | 35.0% | |
| AAGGATCTGAAAGGAAAACT+AGG | + | chr2_2:7973370-7973389 | Msa0235140:CDS | 35.0% | |
| TGAAAGGAAAACTAGGACTT+GGG | + | chr2_2:7973377-7973396 | Msa0235140:CDS | 35.0% | |
| ! | ATGATGCTCAAAATTTGGTG+TGG | + | chr2_2:7973434-7973453 | Msa0235140:CDS | 35.0% |
| !! | TGATGCTCAAAATTTGGTGT+GGG | + | chr2_2:7973435-7973454 | Msa0235140:CDS | 35.0% |
| ATCATCTCCTTAAAGGAGTA+TGG | + | chr2_2:7973491-7973510 | Msa0235140:CDS | 35.0% | |
| AAAAGAAAAGCAAAGGAGAG+TGG | + | chr2_2:7972624-7972643 | Msa0235140:CDS | 35.0% | |
| GTCAAATATTGCAAATAGGC+TGG | + | chr2_2:7972670-7972689 | Msa0235140:CDS | 35.0% | |
| TTCATCATGTGTGCAAAGAT+TGG | + | chr2_2:7972714-7972733 | Msa0235140:CDS | 35.0% | |
| CTAAATTTAGCGTCAACCAA+AGG | - | chr2_2:7972755-7972774 | None:intergenic | 35.0% | |
| TTGGTTGACGCTAAATTTAG+TGG | + | chr2_2:7972755-7972774 | Msa0235140:CDS | 35.0% | |
| ! | AATTTAGTGGATGTGATCCA+TGG | + | chr2_2:7972768-7972787 | Msa0235140:CDS | 35.0% |
| TTTCTGTTGTATGGTGAAGA+AGG | + | chr2_2:7972791-7972810 | Msa0235140:CDS | 35.0% | |
| GATTCTGTGATAGGACAATT+TGG | - | chr2_2:7972980-7972999 | None:intergenic | 35.0% | |
| AGGTGTGCATTCTTTATTGA+AGG | + | chr2_2:7973172-7973191 | Msa0235140:CDS | 35.0% | |
| GGTGTGCATTCTTTATTGAA+GGG | + | chr2_2:7973173-7973192 | Msa0235140:CDS | 35.0% | |
| GTGTGCATTCTTTATTGAAG+GGG | + | chr2_2:7973174-7973193 | Msa0235140:CDS | 35.0% | |
| ACGTTGCTAATACCAAACAA+TGG | + | chr2_2:7973236-7973255 | Msa0235140:CDS | 35.0% | |
| AAGGATCTGAAAGGAAAACT+AGG | + | chr2_2:7973370-7973389 | Msa0235140:CDS | 35.0% | |
| TGAAAGGAAAACTAGGACTT+GGG | + | chr2_2:7973377-7973396 | Msa0235140:CDS | 35.0% | |
| ! | ATGATGCTCAAAATTTGGTG+TGG | + | chr2_2:7973434-7973453 | Msa0235140:CDS | 35.0% |
| !! | TGATGCTCAAAATTTGGTGT+GGG | + | chr2_2:7973435-7973454 | Msa0235140:CDS | 35.0% |
| ATCATCTCCTTAAAGGAGTA+TGG | + | chr2_2:7973491-7973510 | Msa0235140:CDS | 35.0% | |
| GATCCATGGTTTCTGTTGTA+TGG | + | chr2_2:7972782-7972801 | Msa0235140:CDS | 40.0% | |
| TCACCATACAACAGAAACCA+TGG | - | chr2_2:7972788-7972807 | None:intergenic | 40.0% | |
| TTGTTTAGCATGGAACAACG+AGG | - | chr2_2:7972818-7972837 | None:intergenic | 40.0% | |
| TCGTTGTTCCATGCTAAACA+AGG | + | chr2_2:7972817-7972836 | Msa0235140:CDS | 40.0% | |
| CACTATCGAAATTCCAGAAC+TGG | + | chr2_2:7972853-7972872 | Msa0235140:CDS | 40.0% | |
| CAGTCATTCGATTCTGTGAT+AGG | - | chr2_2:7972989-7973008 | None:intergenic | 40.0% | |
| TTGAGTGGCGTGATTAATCA+CGG | - | chr2_2:7973059-7973078 | None:intergenic | 40.0% | |
| TTTGTCGGGGAGATAATTCA+TGG | + | chr2_2:7973101-7973120 | Msa0235140:CDS | 40.0% | |
| ATGGAGCGAGTGTAGTTTAT+TGG | + | chr2_2:7973255-7973274 | Msa0235140:CDS | 40.0% | |
| !! | AAAGGGAAAGCATCGATCAA+AGG | + | chr2_2:7973351-7973370 | Msa0235140:CDS | 40.0% |
| CATCGATCAAAGGATCTGAA+AGG | + | chr2_2:7973361-7973380 | Msa0235140:CDS | 40.0% | |
| CTGAAAGGAAAACTAGGACT+TGG | + | chr2_2:7973376-7973395 | Msa0235140:CDS | 40.0% | |
| GAAAGGAAAACTAGGACTTG+GGG | + | chr2_2:7973378-7973397 | Msa0235140:CDS | 40.0% | |
| !! | GATGCTCAAAATTTGGTGTG+GGG | + | chr2_2:7973436-7973455 | Msa0235140:CDS | 40.0% |
| GATCCATGGTTTCTGTTGTA+TGG | + | chr2_2:7972782-7972801 | Msa0235140:CDS | 40.0% | |
| TCACCATACAACAGAAACCA+TGG | - | chr2_2:7972788-7972807 | None:intergenic | 40.0% | |
| TTGTTTAGCATGGAACAACG+AGG | - | chr2_2:7972818-7972837 | None:intergenic | 40.0% | |
| TCGTTGTTCCATGCTAAACA+AGG | + | chr2_2:7972817-7972836 | Msa0235140:CDS | 40.0% | |
| CACTATCGAAATTCCAGAAC+TGG | + | chr2_2:7972853-7972872 | Msa0235140:CDS | 40.0% | |
| CAGTCATTCGATTCTGTGAT+AGG | - | chr2_2:7972989-7973008 | None:intergenic | 40.0% | |
| TTGAGTGGCGTGATTAATCA+CGG | - | chr2_2:7973059-7973078 | None:intergenic | 40.0% | |
| TTTGTCGGGGAGATAATTCA+TGG | + | chr2_2:7973101-7973120 | Msa0235140:CDS | 40.0% | |
| ATGGAGCGAGTGTAGTTTAT+TGG | + | chr2_2:7973255-7973274 | Msa0235140:CDS | 40.0% | |
| !! | AAAGGGAAAGCATCGATCAA+AGG | + | chr2_2:7973351-7973370 | Msa0235140:CDS | 40.0% |
| CATCGATCAAAGGATCTGAA+AGG | + | chr2_2:7973361-7973380 | Msa0235140:CDS | 40.0% | |
| CTGAAAGGAAAACTAGGACT+TGG | + | chr2_2:7973376-7973395 | Msa0235140:CDS | 40.0% | |
| GAAAGGAAAACTAGGACTTG+GGG | + | chr2_2:7973378-7973397 | Msa0235140:CDS | 40.0% | |
| !! | GATGCTCAAAATTTGGTGTG+GGG | + | chr2_2:7973436-7973455 | Msa0235140:CDS | 40.0% |
| ATCGAAATTCCAGAACTGGC+TGG | + | chr2_2:7972857-7972876 | Msa0235140:CDS | 45.0% | |
| TCGAAATTCCAGAACTGGCT+GGG | + | chr2_2:7972858-7972877 | Msa0235140:CDS | 45.0% | |
| ACAATGCAATCCTGAGACGT+GGG | - | chr2_2:7973034-7973053 | None:intergenic | 45.0% | |
| AACAATGCAATCCTGAGACG+TGG | - | chr2_2:7973035-7973054 | None:intergenic | 45.0% | |
| TAGAACTGCACACGCTTTGT+CGG | + | chr2_2:7973086-7973105 | Msa0235140:CDS | 45.0% | |
| ACTACACTCGCTCCATTGTT+TGG | - | chr2_2:7973251-7973270 | None:intergenic | 45.0% | |
| TGGTGTGGGGTGATTACATT+TGG | + | chr2_2:7973449-7973468 | Msa0235140:CDS | 45.0% | |
| GCACGATCCATACTCCTTTA+AGG | - | chr2_2:7973501-7973520 | None:intergenic | 45.0% | |
| ATCGAAATTCCAGAACTGGC+TGG | + | chr2_2:7972857-7972876 | Msa0235140:CDS | 45.0% | |
| TCGAAATTCCAGAACTGGCT+GGG | + | chr2_2:7972858-7972877 | Msa0235140:CDS | 45.0% | |
| ACAATGCAATCCTGAGACGT+GGG | - | chr2_2:7973034-7973053 | None:intergenic | 45.0% | |
| AACAATGCAATCCTGAGACG+TGG | - | chr2_2:7973035-7973054 | None:intergenic | 45.0% | |
| TAGAACTGCACACGCTTTGT+CGG | + | chr2_2:7973086-7973105 | Msa0235140:CDS | 45.0% | |
| ACTACACTCGCTCCATTGTT+TGG | - | chr2_2:7973251-7973270 | None:intergenic | 45.0% | |
| TGGTGTGGGGTGATTACATT+TGG | + | chr2_2:7973449-7973468 | Msa0235140:CDS | 45.0% | |
| GCACGATCCATACTCCTTTA+AGG | - | chr2_2:7973501-7973520 | None:intergenic | 45.0% | |
| ACTTGCCTTGCATCCTACGA+CGG | + | chr2_2:7972884-7972903 | Msa0235140:CDS | 50.0% | |
| AGAACTGCACACGCTTTGTC+GGG | + | chr2_2:7973087-7973106 | Msa0235140:CDS | 50.0% | |
| ACTTGCCTTGCATCCTACGA+CGG | + | chr2_2:7972884-7972903 | Msa0235140:CDS | 50.0% | |
| AGAACTGCACACGCTTTGTC+GGG | + | chr2_2:7973087-7973106 | Msa0235140:CDS | 50.0% | |
| ! | CAAGTTGACCCAGCCAGTTC+TGG | - | chr2_2:7972869-7972888 | None:intergenic | 55.0% |
| TTCATCTGCTCCCACGTCTC+AGG | + | chr2_2:7973021-7973040 | Msa0235140:CDS | 55.0% | |
| GAACTGCACACGCTTTGTCG+GGG | + | chr2_2:7973088-7973107 | Msa0235140:CDS | 55.0% | |
| ! | CAAGTTGACCCAGCCAGTTC+TGG | - | chr2_2:7972869-7972888 | None:intergenic | 55.0% |
| TTCATCTGCTCCCACGTCTC+AGG | + | chr2_2:7973021-7973040 | Msa0235140:CDS | 55.0% | |
| GAACTGCACACGCTTTGTCG+GGG | + | chr2_2:7973088-7973107 | Msa0235140:CDS | 55.0% | |
| GCGCTCTGCGACTGAACCTT+TGG | + | chr2_2:7972736-7972755 | Msa0235140:CDS | 60.0% | |
| GCCTTGCATCCTACGACGGA+TGG | + | chr2_2:7972888-7972907 | Msa0235140:CDS | 60.0% | |
| GCCATCCGTCGTAGGATGCA+AGG | - | chr2_2:7972892-7972911 | None:intergenic | 60.0% | |
| GAGAAGCAGCCATCCGTCGT+AGG | - | chr2_2:7972900-7972919 | None:intergenic | 60.0% | |
| GCGCTCTGCGACTGAACCTT+TGG | + | chr2_2:7972736-7972755 | Msa0235140:CDS | 60.0% | |
| GCCTTGCATCCTACGACGGA+TGG | + | chr2_2:7972888-7972907 | Msa0235140:CDS | 60.0% | |
| GCCATCCGTCGTAGGATGCA+AGG | - | chr2_2:7972892-7972911 | None:intergenic | 60.0% | |
| GAGAAGCAGCCATCCGTCGT+AGG | - | chr2_2:7972900-7972919 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_2 | gene | 7972596 | 7973540 | 7972596 | ID=Msa0235140;Name=Msa0235140 |
| chr2_2 | mRNA | 7972596 | 7973540 | 7972596 | ID=Msa0235140-mRNA-1;Parent=Msa0235140;Name=Msa0235140-mRNA-1;_AED=0.00;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|314 |
| chr2_2 | exon | 7972596 | 7973540 | 7972596 | ID=Msa0235140-mRNA-1:exon:3453;Parent=Msa0235140-mRNA-1 |
| chr2_2 | CDS | 7972596 | 7973540 | 7972596 | ID=Msa0235140-mRNA-1:cds;Parent=Msa0235140-mRNA-1 |
| Gene Sequence |
| Protein sequence |
