AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1355970

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1355970 MtrunA17_Chr3g0136731 84.615 52 1 1 1 45 1 52 5.03e-24 84.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 42 sgRNAs with CRISPR-Local

Find 257 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCAACACTCGCTCTCTTAT+TGG 0.189980 tig0015260:-1700 Msa1355970:three_prime_UTR
CCGATTTACCGCAGGTATTT+TGG 0.194402 tig0015260:-4804 Msa1355970:intron
GGTAGAATATTCACCTAATC+AGG 0.266410 tig0015260:-1818 Msa1355970:three_prime_UTR
AGCTGATTGTTCTGGGAAAT+TGG 0.293695 tig0015260:-2140 Msa1355970:three_prime_UTR
GTTAATTTGTTGGTATGCCT+TGG 0.359869 tig0015260:-1894 Msa1355970:three_prime_UTR
TATGATACTTGTTAATTTGT+TGG 0.367418 tig0015260:-1904 Msa1355970:three_prime_UTR
ATTAGAAGAACGAGAAATAA+AGG 0.372863 tig0015260:+4937 None:intergenic
AGTTACAAGTTTGCATCCTT+TGG 0.374388 tig0015260:-2180 Msa1355970:three_prime_UTR
CGACTCTTATCACTTTATAT+AGG 0.377946 tig0015260:+1635 None:intergenic
CTAATAGCAGCTCATGTTCT+CGG 0.382026 tig0015260:-4919 Msa1355970:CDS
TTAATTTGTTGGTATGCCTT+GGG 0.390776 tig0015260:-1893 Msa1355970:three_prime_UTR
CCCTGGACGCAATAGAACTT+TGG 0.432262 tig0015260:+5077 None:intergenic
ATTGCGTCCAGGGTTCAGTT+GGG 0.433906 tig0015260:-5067 Msa1355970:five_prime_UTR
AATAACACATCACCCTGATT+AGG 0.435564 tig0015260:+1805 None:intergenic
CCTGGACGCAATAGAACTTT+GGG 0.446142 tig0015260:+5078 None:intergenic
TATTGCGTCCAGGGTTCAGT+TGG 0.448218 tig0015260:-5068 Msa1355970:five_prime_UTR
CCCAAAGTTCTATTGCGTCC+AGG 0.467958 tig0015260:-5078 Msa1355970:five_prime_UTR
AGCGAGTGTTGGTGAGATTG+TGG 0.469667 tig0015260:+1710 None:intergenic
AGCAGCTCATGTTCTCGGTC+TGG 0.477055 tig0015260:-4914 Msa1355970:intron
CTTGCGCCATTGTACAAAAC+GGG 0.483536 tig0015260:+4980 None:intergenic
TTTCATTTGTTGAACTGTGA+AGG 0.489687 tig0015260:-5042 Msa1355970:five_prime_UTR
CCAAAGTTCTATTGCGTCCA+GGG 0.490923 tig0015260:-5077 Msa1355970:five_prime_UTR
GCTCTCTTATTGGATGATAT+CGG 0.497090 tig0015260:-1690 Msa1355970:three_prime_UTR
TAACTCACTGCTTAGATGAT+CGG 0.503644 tig0015260:+1758 None:intergenic
TCTTGCGCCATTGTACAAAA+CGG 0.509754 tig0015260:+4979 None:intergenic
TTGTTGAACTGTGAAGGATC+TGG 0.525998 tig0015260:-5036 Msa1355970:five_prime_UTR
TCTGGGAAATTGGCAATGCT+CGG 0.550050 tig0015260:-2130 Msa1355970:three_prime_UTR
TGAAAACCCCAACTGAACCC+TGG 0.556509 tig0015260:+5060 None:intergenic
TTGCGTCCAGGGTTCAGTTG+GGG 0.562869 tig0015260:-5066 Msa1355970:five_prime_UTR
AAATTGGCAATGCTCGGAAG+TGG 0.564571 tig0015260:-2124 Msa1355970:three_prime_UTR
TGAGCCTTTCGCCTCTGCAG+TGG 0.573093 tig0015260:+4754 None:intergenic
ACGAGAAATAAAGGAACTGC+CGG 0.582979 tig0015260:+4946 None:intergenic
TCCAATAAGAGAGCGAGTGT+TGG 0.598655 tig0015260:+1699 None:intergenic
ATTCTTAAACATGCAGCCCA+AGG 0.606729 tig0015260:+1877 None:intergenic
GGCGCAAGATCAATACTCAC+CGG 0.610616 tig0015260:-4965 Msa1355970:CDS
GGACGCAATAGAACTTTGGG+TGG 0.611940 tig0015260:+5081 None:intergenic
GTAGAATATTCACCTAATCA+GGG 0.617357 tig0015260:-1817 Msa1355970:three_prime_UTR
GACGCAATAGAACTTTGGGT+GGG 0.618603 tig0015260:+5082 None:intergenic
AACAATCTCAGAAGACCTTG+AGG 0.620821 tig0015260:+2076 None:intergenic
ACAACCACTGCAGAGGCGAA+AGG 0.629999 tig0015260:-4758 Msa1355970:CDS
CTCAGAAACAACCACTGCAG+AGG 0.630477 tig0015260:-4765 Msa1355970:CDS
GAAAGGCTCACTGAAAGAGA+AGG 0.649077 tig0015260:-4741 Msa1355970:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTAAAATTGTTATTTATTTA+TGG - tig0015260:3693-3712 Msa1355970:intron 10.0%
!!! GTAATTGTTTTCAATTTTAT+AGG - tig0015260:2045-2064 Msa1355970:three_prime_UTR 15.0%
!!! TTTGATTTTTCAAAATTGAT+AGG - tig0015260:2179-2198 Msa1355970:three_prime_UTR 15.0%
!!! ATAGAAACTAGTAATTTTTT+TGG - tig0015260:2323-2342 Msa1355970:intron 15.0%
!!! ATTGAAAAACTCAAATTTTT+TGG + tig0015260:2907-2926 None:intergenic 15.0%
!!! AAAATTTGAGTTTTTCAATT+TGG - tig0015260:2908-2927 Msa1355970:intron 15.0%
!!! AAATTTGAGTTTTTCAATTT+GGG - tig0015260:2909-2928 Msa1355970:intron 15.0%
!! AATATATTTCTCAATAAAGT+TGG - tig0015260:3366-3385 Msa1355970:intron 15.0%
!! TAAATAAAGTAATTTAACCT+TGG - tig0015260:3757-3776 Msa1355970:intron 15.0%
!!! GATAAATTTTTAAGCTTTTA+AGG + tig0015260:3800-3819 None:intergenic 15.0%
!! TTATTTGAGAATAGATGTAA+GGG + tig0015260:1891-1910 None:intergenic 20.0%
!! GATTTGAACTATAATGTAAA+TGG - tig0015260:2089-2108 Msa1355970:three_prime_UTR 20.0%
!!! CTAGTTTCTATATATGATTT+TGG + tig0015260:2314-2333 None:intergenic 20.0%
!! GTATTTGTAAATAAACTAGT+AGG + tig0015260:2395-2414 None:intergenic 20.0%
!! AAAAAATTTCTATTGATGAG+AGG - tig0015260:2740-2759 Msa1355970:intron 20.0%
!! AAAAATTTCTATTGATGAGA+GGG - tig0015260:2741-2760 Msa1355970:intron 20.0%
!!! AATTTGAGTTTTTCAATTTG+GGG - tig0015260:2910-2929 Msa1355970:intron 20.0%
!! AAGTATATAAACCTATCAAT+CGG + tig0015260:3411-3430 None:intergenic 20.0%
!!! ACATTATATTTTATGTCTGT+AGG - tig0015260:3450-3469 Msa1355970:intron 20.0%
!!! ACTTCTTTCTTTGTTTATTT+GGG - tig0015260:4241-4260 Msa1355970:intron 20.0%
!!! TATGATACTTGTTAATTTGT+TGG - tig0015260:4823-4842 Msa1355970:intron 20.0%
! ATTAGAAGAACGAGAAATAA+AGG + tig0015260:1793-1812 None:intergenic 25.0%
! TATTTGAGAATAGATGTAAG+GGG + tig0015260:1890-1909 None:intergenic 25.0%
! GTTATTTGAGAATAGATGTA+AGG + tig0015260:1892-1911 None:intergenic 25.0%
! TTATAAATCCAAAATACCTG+CGG + tig0015260:1934-1953 None:intergenic 25.0%
! ACACTGTCTGATATAAATTA+GGG + tig0015260:2027-2046 None:intergenic 25.0%
! TACACTGTCTGATATAAATT+AGG + tig0015260:2028-2047 None:intergenic 25.0%
! GAACTATAATGTAAATGGAT+CGG - tig0015260:2094-2113 Msa1355970:three_prime_UTR 25.0%
! GTTTAAATTCATATGTTGCT+CGG - tig0015260:2123-2142 Msa1355970:three_prime_UTR 25.0%
!! GTTTCTATATATGATTTTGG+AGG + tig0015260:2311-2330 None:intergenic 25.0%
!! ATATGACTCAGGTATATTTT+GGG - tig0015260:2691-2710 Msa1355970:intron 25.0%
! ATTCATGGGAGATAAATTTA+TGG - tig0015260:2780-2799 Msa1355970:intron 25.0%
! GATATTGCATATTATATGCA+GGG - tig0015260:2807-2826 Msa1355970:intron 25.0%
! ATATTGCATATTATATGCAG+GGG - tig0015260:2808-2827 Msa1355970:intron 25.0%
!! ACTTTTGAATTGCAAAGAAA+AGG + tig0015260:2959-2978 None:intergenic 25.0%
! ACTTCAATTTAACAACATTG+AGG + tig0015260:2998-3017 None:intergenic 25.0%
! ATGTTGTTAAATTGAAGTCA+TGG - tig0015260:3000-3019 Msa1355970:intron 25.0%
!! CATGGAGATTATTTTCTTTT+AGG + tig0015260:3181-3200 None:intergenic 25.0%
! CTAAAAGAAAATAATCTCCA+TGG - tig0015260:3179-3198 Msa1355970:intron 25.0%
!! TTTAACTTCTAGACAATCTT+TGG + tig0015260:3309-3328 None:intergenic 25.0%
! TAATCTAAACTCCCAAAATA+TGG + tig0015260:3522-3541 None:intergenic 25.0%
!! CACTTCTTTCTTTGTTTATT+TGG - tig0015260:4240-4259 Msa1355970:intron 25.0%
! CAGTTGTTAAATTGAAATCT+AGG - tig0015260:4471-4490 Msa1355970:intron 25.0%
!! CAAAAATTTCTTTTCTCCAA+AGG + tig0015260:4566-4585 None:intergenic 25.0%
!!! AATTTTGATCATTTTCCTCA+AGG - tig0015260:4636-4655 Msa1355970:intron 25.0%
!!! CAACTCAAATTTTGTTTTAC+AGG + tig0015260:4758-4777 None:intergenic 25.0%
!!! GTTTTTTGATAGAAGAATAG+TGG - tig0015260:4888-4907 Msa1355970:intron 25.0%
! TTTCATTTGTTGAACTGTGA+AGG - tig0015260:1685-1704 Msa1355970:three_prime_UTR 30.0%
!!! CTGGTTTATTAGGTTTTAAG+AGG - tig0015260:2251-2270 Msa1355970:intron 30.0%
! GAAAGTAAAGTACTCTTACA+GGG - tig0015260:2345-2364 Msa1355970:intron 30.0%
CATGCAAACCTAAAATGTAA+GGG + tig0015260:2531-2550 None:intergenic 30.0%
AAAATAGCATGATATGACTC+AGG - tig0015260:2680-2699 Msa1355970:intron 30.0%
! GATATGACTCAGGTATATTT+TGG - tig0015260:2690-2709 Msa1355970:intron 30.0%
TCAATTGACTAGTACATTCA+TGG - tig0015260:2765-2784 Msa1355970:intron 30.0%
CAATTGACTAGTACATTCAT+GGG - tig0015260:2766-2785 Msa1355970:intron 30.0%
ATGGGAGATAAATTTATGGT+AGG - tig0015260:2784-2803 Msa1355970:intron 30.0%
TGGGAGATAAATTTATGGTA+GGG - tig0015260:2785-2804 Msa1355970:intron 30.0%
GGATATTGCATATTATATGC+AGG - tig0015260:2806-2825 Msa1355970:intron 30.0%
ACAATTAAATTGTGGAGAAG+TGG + tig0015260:2857-2876 None:intergenic 30.0%
AGAAAAGGAGAATCATCAAT+TGG + tig0015260:2944-2963 None:intergenic 30.0%
CTTTGCAATTCAAAAGTTTG+TGG - tig0015260:2962-2981 Msa1355970:intron 30.0%
GCTTTAACAAAATCTGAACT+AGG + tig0015260:3213-3232 None:intergenic 30.0%
ATCCAAAACTTCGATAAAGA+TGG + tig0015260:3235-3254 None:intergenic 30.0%
CTTGAAAAAGTCATAAGTAG+AGG + tig0015260:3266-3285 None:intergenic 30.0%
TTGTTAATGATCCGATTGAT+AGG - tig0015260:3397-3416 Msa1355970:intron 30.0%
TTATGAATGCACAATTTCCA+AGG + tig0015260:3777-3796 None:intergenic 30.0%
AAGGATAAAAGGGCAAAAAA+GGG + tig0015260:4053-4072 None:intergenic 30.0%
TAAGGATAAAAGGGCAAAAA+AGG + tig0015260:4054-4073 None:intergenic 30.0%
ACGAACGGGTATTAAATAAA+GGG + tig0015260:4108-4127 None:intergenic 30.0%
! TTCTTTGTTTATTTGGGAGT+AGG - tig0015260:4247-4266 Msa1355970:intron 30.0%
AATGCATACAATAGACAATG+TGG + tig0015260:4402-4421 None:intergenic 30.0%
!! TTACTTTTGTAGTCCAACTA+GGG - tig0015260:4438-4457 Msa1355970:intron 30.0%
! ATTTTTGTAGCTGATTGTTC+TGG - tig0015260:4579-4598 Msa1355970:intron 30.0%
! TTTTTGTAGCTGATTGTTCT+GGG - tig0015260:4580-4599 Msa1355970:intron 30.0%
!!! AGTTGATGAAGTGCATTTTT+AGG - tig0015260:4773-4792 Msa1355970:CDS 30.0%
!! TTAATTTGTTGGTATGCCTT+GGG - tig0015260:4834-4853 Msa1355970:intron 30.0%
GTAGAATATTCACCTAATCA+GGG - tig0015260:4910-4929 Msa1355970:intron 30.0%
CTGGAAGAAAAAAGAAACAC+TGG - tig0015260:1710-1729 Msa1355970:three_prime_UTR 35.0%
AACTACACACGATGAACTAA+AGG + tig0015260:2206-2225 None:intergenic 35.0%
AAACCTAATAAACCAGAACG+CGG + tig0015260:2247-2266 None:intergenic 35.0%
!!! GGTTTTAAGAGGATCTATGT+AGG - tig0015260:2262-2281 Msa1355970:intron 35.0%
! GGAAAGTAAAGTACTCTTAC+AGG - tig0015260:2344-2363 Msa1355970:intron 35.0%
! ATCGTTTTCGTAGCTCATAT+AGG - tig0015260:2449-2468 Msa1355970:intron 35.0%
!! GAGCATTGAAATTGATGATC+TGG - tig0015260:2490-2509 Msa1355970:intron 35.0%
! TTTAGCGTCCCTTACATTTT+AGG - tig0015260:2520-2539 Msa1355970:intron 35.0%
CCATGCAAACCTAAAATGTA+AGG + tig0015260:2532-2551 None:intergenic 35.0%
!!! CCTTACATTTTAGGTTTGCA+TGG - tig0015260:2529-2548 Msa1355970:intron 35.0%
! AGTACGAGCAAGTTTGAAAT+AGG - tig0015260:2625-2644 Msa1355970:intron 35.0%
GAGCTCACACAATTAAATTG+TGG + tig0015260:2865-2884 None:intergenic 35.0%
!! TTTTTGGTCAAGTAGACTAG+TGG + tig0015260:2891-2910 None:intergenic 35.0%
CAAAAGTTTGTGGTTCCTAT+TGG - tig0015260:2972-2991 Msa1355970:intron 35.0%
TTAACAACATTGAGGCCAAT+AGG + tig0015260:2990-3009 None:intergenic 35.0%
ATTCCTTCACAAACTTCCTA+CGG + tig0015260:3068-3087 None:intergenic 35.0%
!!! GTGTTTATATGGCGGATTTT+CGG - tig0015260:3121-3140 Msa1355970:intron 35.0%
AAAACCAATGTTGTCAACAG+CGG + tig0015260:3158-3177 None:intergenic 35.0%
! AGCCATCTTTATCGAAGTTT+TGG - tig0015260:3230-3249 Msa1355970:intron 35.0%
AGTGCACTATTAACGTTACA+AGG + tig0015260:3483-3502 None:intergenic 35.0%
CTAAACTCCCAAAATATGGT+GGG + tig0015260:3518-3537 None:intergenic 35.0%
TCTAAACTCCCAAAATATGG+TGG + tig0015260:3519-3538 None:intergenic 35.0%
GGCACAAGATAAGGATAAAA+GGG + tig0015260:4063-4082 None:intergenic 35.0%
! AATGCTAATTTGTGCCCTTA+GGG + tig0015260:4084-4103 None:intergenic 35.0%
! AAATGCTAATTTGTGCCCTT+AGG + tig0015260:4085-4104 None:intergenic 35.0%
CACGAACGGGTATTAAATAA+AGG + tig0015260:4109-4128 None:intergenic 35.0%
TACAATAGACAATGTGGACT+TGG + tig0015260:4396-4415 None:intergenic 35.0%
!! CTTACTTTTGTAGTCCAACT+AGG - tig0015260:4437-4456 Msa1355970:intron 35.0%
AGTTACAAGTTTGCATCCTT+TGG - tig0015260:4547-4566 Msa1355970:intron 35.0%
!! TGATGAAGTGCATTTTTAGG+AGG - tig0015260:4776-4795 Msa1355970:CDS 35.0%
!! GTTAATTTGTTGGTATGCCT+TGG - tig0015260:4833-4852 Msa1355970:intron 35.0%
GGTAGAATATTCACCTAATC+AGG - tig0015260:4909-4928 Msa1355970:intron 35.0%
AATAACACATCACCCTGATT+AGG + tig0015260:4925-4944 None:intergenic 35.0%
TAACTCACTGCTTAGATGAT+CGG + tig0015260:4972-4991 None:intergenic 35.0%
GCTCTCTTATTGGATGATAT+CGG - tig0015260:5037-5056 Msa1355970:five_prime_UTR 35.0%
TTGTTGAACTGTGAAGGATC+TGG - tig0015260:1691-1710 Msa1355970:three_prime_UTR 40.0%
! CTTCTTCCCGTTTTGTACAA+TGG - tig0015260:1741-1760 Msa1355970:three_prime_UTR 40.0%
TCTTGCGCCATTGTACAAAA+CGG + tig0015260:1751-1770 None:intergenic 40.0%
ACGAGAAATAAAGGAACTGC+CGG + tig0015260:1784-1803 None:intergenic 40.0%
CTAATAGCAGCTCATGTTCT+CGG - tig0015260:1808-1827 Msa1355970:three_prime_UTR 40.0%
TGAAATGAAGTGAGGGAAAG+TGG + tig0015260:1851-1870 None:intergenic 40.0%
GGTAGCATGAAATGAAGTGA+GGG + tig0015260:1858-1877 None:intergenic 40.0%
TGGTAGCATGAAATGAAGTG+AGG + tig0015260:1859-1878 None:intergenic 40.0%
GAGAATAGATGTAAGGGGAA+TGG + tig0015260:1885-1904 None:intergenic 40.0%
CCAAAATACCTGCGGTAAAT+CGG + tig0015260:1926-1945 None:intergenic 40.0%
AACCTAATAAACCAGAACGC+GGG + tig0015260:2246-2265 None:intergenic 40.0%
AATGTAAGGGACGCTAAAGA+TGG + tig0015260:2518-2537 None:intergenic 40.0%
GTAGGAAGTTTGTGAAGGAA+TGG - tig0015260:3067-3086 Msa1355970:intron 40.0%
TCCGCAATGGTGTGTTTATA+TGG - tig0015260:3110-3129 Msa1355970:intron 40.0%
GCCATATAAACACACCATTG+CGG + tig0015260:3114-3133 None:intergenic 40.0%
GCAATGGTGTGTTTATATGG+CGG - tig0015260:3113-3132 Msa1355970:intron 40.0%
! TGAACTAGGTTGAGTATCCA+TGG + tig0015260:3199-3218 None:intergenic 40.0%
! TCCATTGCCCACCATATTTT+GGG - tig0015260:3508-3527 Msa1355970:intron 40.0%
TTGAGTCAGCCTTGGAAATA+TGG - tig0015260:3612-3631 Msa1355970:intron 40.0%
TTCTTATCCTTGTGCCCTTA+GGG + tig0015260:3735-3754 None:intergenic 40.0%
TTTCTTATCCTTGTGCCCTT+AGG + tig0015260:3736-3755 None:intergenic 40.0%
GTTGTTGTCATGTGACTGAA+AGG - tig0015260:3904-3923 Msa1355970:intron 40.0%
AACAGCCTCTTGTGTAAAAC+AGG - tig0015260:3947-3966 Msa1355970:intron 40.0%
ACAGCCTCTTGTGTAAAACA+GGG - tig0015260:3948-3967 Msa1355970:intron 40.0%
! CTTACCCTGTTTTACACAAG+AGG + tig0015260:3955-3974 None:intergenic 40.0%
CTCTTGTGTAAAACAGGGTA+AGG - tig0015260:3953-3972 Msa1355970:intron 40.0%
CGTACAATACACCAAATGGT+GGG - tig0015260:3979-3998 Msa1355970:intron 40.0%
GGGCACAAGATAAGGATAAA+AGG + tig0015260:4064-4083 None:intergenic 40.0%
TATCCTTATCTTGTGCCCTA+AGG - tig0015260:4066-4085 Msa1355970:intron 40.0%
ATCCTTATCTTGTGCCCTAA+GGG - tig0015260:4067-4086 Msa1355970:intron 40.0%
AACTGCATTAAACAGCACAC+AGG + tig0015260:4135-4154 None:intergenic 40.0%
ATGCAGTTTGTGCCAAACTA+AGG - tig0015260:4147-4166 Msa1355970:intron 40.0%
CTCAAGTTAGAGCCTTAGTT+TGG + tig0015260:4162-4181 None:intergenic 40.0%
TTTATTTGGGAGTAGGAGTG+TGG - tig0015260:4254-4273 Msa1355970:intron 40.0%
! TTATTTGGGAGTAGGAGTGT+GGG - tig0015260:4255-4274 Msa1355970:intron 40.0%
! GGTCTGGAGTTAACTAAAGA+TGG + tig0015260:4370-4389 None:intergenic 40.0%
!! AGCTGATTGTTCTGGGAAAT+TGG - tig0015260:4587-4606 Msa1355970:intron 40.0%
AACAATCTCAGAAGACCTTG+AGG + tig0015260:4654-4673 None:intergenic 40.0%
! TGTTTTACAGGAAGAACGAC+TGG + tig0015260:4746-4765 None:intergenic 40.0%
ATTCTTAAACATGCAGCCCA+AGG + tig0015260:4853-4872 None:intergenic 40.0%
GACGCAATAGAACTTTGGGT+GGG + tig0015260:1648-1667 None:intergenic 45.0%
CCTGGACGCAATAGAACTTT+GGG + tig0015260:1652-1671 None:intergenic 45.0%
CCAAAGTTCTATTGCGTCCA+GGG - tig0015260:1650-1669 Msa1355970:three_prime_UTR 45.0%
CTTGCGCCATTGTACAAAAC+GGG + tig0015260:1750-1769 None:intergenic 45.0%
AGATGTAAGGGGAATGGAAC+TGG + tig0015260:1879-1898 None:intergenic 45.0%
! CCGATTTACCGCAGGTATTT+TGG - tig0015260:1923-1942 Msa1355970:three_prime_UTR 45.0%
GAAAGGCTCACTGAAAGAGA+AGG - tig0015260:1986-2005 Msa1355970:three_prime_UTR 45.0%
ACCTAATAAACCAGAACGCG+GGG + tig0015260:2245-2264 None:intergenic 45.0%
TCTTACAGGGCTCATCAGTT+TGG - tig0015260:2358-2377 Msa1355970:intron 45.0%
GAGCTATGTAGCATGCATGA+TGG - tig0015260:2573-2592 Msa1355970:intron 45.0%
TGCATATTATATGCAGGGGC+CGG - tig0015260:2812-2831 Msa1355970:intron 45.0%
!! GGAATGCCACGATGGATTTT+TGG - tig0015260:3035-3054 Msa1355970:intron 45.0%
GGTTGTCCAAAAATCCATCG+TGG + tig0015260:3044-3063 None:intergenic 45.0%
!! GATTTTTGGACAACCACCGT+AGG - tig0015260:3049-3068 Msa1355970:intron 45.0%
! AAACACACCATTGCGGACTA+TGG + tig0015260:3107-3126 None:intergenic 45.0%
! GTCCATTGCCCACCATATTT+TGG - tig0015260:3507-3526 Msa1355970:intron 45.0%
!! TCCCAAAATATGGTGGGCAA+TGG + tig0015260:3512-3531 None:intergenic 45.0%
GCTCGAAAAATGCACACAAC+AGG + tig0015260:3588-3607 None:intergenic 45.0%
! TTTCCAAGGCTGACTCAAGA+AGG + tig0015260:3610-3629 None:intergenic 45.0%
AGTCAGCCTTGGAAATATGG+TGG - tig0015260:3615-3634 Msa1355970:intron 45.0%
GTACAGCCACCATATTTCCA+AGG + tig0015260:3624-3643 None:intergenic 45.0%
TCTTGCTAACTTCTGCCCTA+AGG - tig0015260:3717-3736 Msa1355970:intron 45.0%
CTTGCTAACTTCTGCCCTAA+GGG - tig0015260:3718-3737 Msa1355970:intron 45.0%
ATCCTTATCTTGTGCCCTGA+GGG - tig0015260:3858-3877 Msa1355970:intron 45.0%
GTCATGTGACTGAAAGGTCA+CGG - tig0015260:3910-3929 Msa1355970:intron 45.0%
TCATGTGACTGAAAGGTCAC+GGG - tig0015260:3911-3930 Msa1355970:intron 45.0%
! TTACACAAGAGGCTGTTTCC+AGG + tig0015260:3944-3963 None:intergenic 45.0%
GCTGCGTACAATACACCAAA+TGG - tig0015260:3975-3994 Msa1355970:intron 45.0%
GCGTACAATACACCAAATGG+TGG - tig0015260:3978-3997 Msa1355970:intron 45.0%
TAGACAATGTGGACTTGGAC+TGG + tig0015260:4391-4410 None:intergenic 45.0%
TCTGGGAAATTGGCAATGCT+CGG - tig0015260:4597-4616 Msa1355970:intron 45.0%
AAATTGGCAATGCTCGGAAG+TGG - tig0015260:4603-4622 Msa1355970:intron 45.0%
ACCAACACTCGCTCTCTTAT+TGG - tig0015260:5027-5046 Msa1355970:five_prime_UTR 45.0%
TCCAATAAGAGAGCGAGTGT+TGG + tig0015260:5031-5050 None:intergenic 45.0%
!!! TAAAATTGTTATTTATTTAT+GGG - tig0015260:3694-3713 Msa1355970:intron 5.0%
GGACGCAATAGAACTTTGGG+TGG + tig0015260:1649-1668 None:intergenic 50.0%
CCCAAAGTTCTATTGCGTCC+AGG - tig0015260:1649-1668 Msa1355970:three_prime_UTR 50.0%
CCCTGGACGCAATAGAACTT+TGG + tig0015260:1653-1672 None:intergenic 50.0%
TATTGCGTCCAGGGTTCAGT+TGG - tig0015260:1659-1678 Msa1355970:three_prime_UTR 50.0%
ATTGCGTCCAGGGTTCAGTT+GGG - tig0015260:1660-1679 Msa1355970:three_prime_UTR 50.0%
TGAAAACCCCAACTGAACCC+TGG + tig0015260:1670-1689 None:intergenic 50.0%
GGCGCAAGATCAATACTCAC+CGG - tig0015260:1762-1781 Msa1355970:three_prime_UTR 50.0%
! GTGCTTTTCCGATTTACCGC+AGG - tig0015260:1915-1934 Msa1355970:three_prime_UTR 50.0%
CTCAGAAACAACCACTGCAG+AGG - tig0015260:1962-1981 Msa1355970:three_prime_UTR 50.0%
GGCTCACTGAAAGAGAAGGT+TGG - tig0015260:1990-2009 Msa1355970:three_prime_UTR 50.0%
!! ACCCCGCGTTCTGGTTTATT+AGG - tig0015260:2241-2260 Msa1355970:intron 50.0%
GGGTGCGCAATTCTCTAATC+TGG - tig0015260:2711-2730 Msa1355970:intron 50.0%
GCATATTATATGCAGGGGCC+GGG - tig0015260:2813-2832 Msa1355970:intron 50.0%
CATATTATATGCAGGGGCCG+GGG - tig0015260:2814-2833 Msa1355970:intron 50.0%
! TTGTGGAGAAGTGGAGTGTC+CGG + tig0015260:2848-2867 None:intergenic 50.0%
!! TTGAAGTCATGGTGCTGCCA+TGG - tig0015260:3011-3030 Msa1355970:intron 50.0%
CCTTCACAAACTTCCTACGG+TGG + tig0015260:3065-3084 None:intergenic 50.0%
CCACCGTAGGAAGTTTGTGA+AGG - tig0015260:3062-3081 Msa1355970:intron 50.0%
TTGTGAAGGAATGGCTGCCA+TGG - tig0015260:3076-3095 Msa1355970:intron 50.0%
ATGTTGTCAACAGCGGATGG+CGG + tig0015260:3151-3170 None:intergenic 50.0%
CCAATGTTGTCAACAGCGGA+TGG + tig0015260:3154-3173 None:intergenic 50.0%
CCATCCGCTGTTGACAACAT+TGG - tig0015260:3151-3170 Msa1355970:intron 50.0%
GATCCTTATCTTGTGCCCTG+AGG - tig0015260:3857-3876 Msa1355970:intron 50.0%
TCCTTATCTTGTGCCCTGAG+GGG - tig0015260:3859-3878 Msa1355970:intron 50.0%
CAACTTTACCAGTTGCGCCA+AGG + tig0015260:3888-3907 None:intergenic 50.0%
AAGGGCAAAAAAGGGCAACC+CGG + tig0015260:4045-4064 None:intergenic 50.0%
TGCCCTTAGGGCACAAGATA+AGG + tig0015260:4072-4091 None:intergenic 50.0%
!! GAAAGAAGTGCTGCGAGTGA+TGG + tig0015260:4230-4249 None:intergenic 50.0%
! GAGTAGGAGTGTGGGTCTTA+TGG - tig0015260:4263-4282 Msa1355970:intron 50.0%
!! AATGTGGACTTGGACTGGTC+TGG + tig0015260:4386-4405 None:intergenic 50.0%
!! AGCGAGTGTTGGTGAGATTG+TGG + tig0015260:5020-5039 None:intergenic 50.0%
TTGCGTCCAGGGTTCAGTTG+GGG - tig0015260:1661-1680 Msa1355970:three_prime_UTR 55.0%
AGCAGCTCATGTTCTCGGTC+TGG - tig0015260:1813-1832 Msa1355970:three_prime_UTR 55.0%
ACAACCACTGCAGAGGCGAA+AGG - tig0015260:1969-1988 Msa1355970:three_prime_UTR 55.0%
TGATCTACAACCCCGCGTTC+TGG - tig0015260:2232-2251 Msa1355970:intron 55.0%
! TGTGGAGAAGTGGAGTGTCC+GGG + tig0015260:2847-2866 None:intergenic 55.0%
!! AAGTCATGGTGCTGCCATGG+TGG - tig0015260:3014-3033 Msa1355970:intron 55.0%
TCCATCGTGGCATTCCACCA+TGG + tig0015260:3031-3050 None:intergenic 55.0%
GAGCCTTCTTGAGTCAGCCT+TGG - tig0015260:3604-3623 Msa1355970:intron 55.0%
! ACTTCTGCCCTAAGGGCACA+AGG - tig0015260:3725-3744 Msa1355970:intron 55.0%
ACCCCTCAGGGCACAAGATA+AGG + tig0015260:3863-3882 None:intergenic 55.0%
AAGGTCACGGGTTCAAGTCC+TGG - tig0015260:3923-3942 Msa1355970:intron 55.0%
CTAGAGCTCCCGCATATGCA+GGG + tig0015260:4018-4037 None:intergenic 55.0%
ACTAGAGCTCCCGCATATGC+AGG + tig0015260:4019-4038 None:intergenic 55.0%
AGCACACAGGATGCACGAAC+GGG + tig0015260:4122-4141 None:intergenic 55.0%
CAGCACACAGGATGCACGAA+CGG + tig0015260:4123-4142 None:intergenic 55.0%
CTGATCAGTGCTGCCCTAGT+TGG + tig0015260:4454-4473 None:intergenic 55.0%
TGAGCCTTTCGCCTCTGCAG+TGG + tig0015260:1976-1995 None:intergenic 60.0%
! GTGGAGAAGTGGAGTGTCCG+GGG + tig0015260:2846-2865 None:intergenic 60.0%
! GCCATGGTGGAATGCCACGA+TGG - tig0015260:3027-3046 Msa1355970:intron 60.0%
!! TGTCAACAGCGGATGGCGGA+AGG + tig0015260:3147-3166 None:intergenic 60.0%
TGTGCCCTGAGGGGTAACCT+TGG - tig0015260:3868-3887 Msa1355970:intron 60.0%
TGCGCCAAGGTTACCCCTCA+GGG + tig0015260:3875-3894 None:intergenic 60.0%
TTGCGCCAAGGTTACCCCTC+AGG + tig0015260:3876-3895 None:intergenic 60.0%
AGGGGTAACCTTGGCGCAAC+TGG - tig0015260:3877-3896 Msa1355970:intron 60.0%
! CAAATGGTGGGACCCCTTCC+CGG - tig0015260:3991-4010 Msa1355970:intron 60.0%
CATATGCAGGGTCCGGGAAG+GGG + tig0015260:4006-4025 None:intergenic 60.0%
GCATATGCAGGGTCCGGGAA+GGG + tig0015260:4007-4026 None:intergenic 60.0%
CTTCCCGGACCCTGCATATG+CGG - tig0015260:4006-4025 Msa1355970:intron 60.0%
TTCCCGGACCCTGCATATGC+GGG - tig0015260:4007-4026 Msa1355970:intron 60.0%
! ATGCGGGAGCTCTAGTGCAC+CGG - tig0015260:4023-4042 Msa1355970:intron 60.0%
! TGCGGACTATGGCGCTGCCA+TGG + tig0015260:3096-3115 None:intergenic 65.0%
GGCAGCGCCATAGTCCGCAA+TGG - tig0015260:3097-3116 Msa1355970:intron 65.0%
CGGGAAGGGGTCCCACCATT+TGG + tig0015260:3993-4012 None:intergenic 65.0%
CGCATATGCAGGGTCCGGGA+AGG + tig0015260:4008-4027 None:intergenic 65.0%
CTCCCGCATATGCAGGGTCC+GGG + tig0015260:4012-4031 None:intergenic 65.0%
GCTCCCGCATATGCAGGGTC+CGG + tig0015260:4013-4032 None:intergenic 65.0%
! TGCGGGAGCTCTAGTGCACC+GGG - tig0015260:4024-4043 Msa1355970:intron 65.0%
AGTGTCCGGGGTTCGAGCCC+CGG + tig0015260:2834-2853 None:intergenic 70.0%
! AGGGGCCGGGGCTCGAACCC+CGG - tig0015260:2826-2845 Msa1355970:intron 80.0%
Chromosome Type Strat End Strand Name
tig0015260 gene 1638 5111 1638 ID=Msa1355970;Name=Msa1355970
tig0015260 mRNA 1638 5111 1638 ID=Msa1355970-mRNA-1;Parent=Msa1355970;Name=Msa1355970-mRNA-1;_AED=0.15;_eAED=0.15;_QI=122|1|1|1|0.5|0.33|3|571|45
tig0015260 exon 1638 2200 1638 ID=Msa1355970-mRNA-1:exon:9682;Parent=Msa1355970-mRNA-1
tig0015260 exon 4742 4812 4742 ID=Msa1355970-mRNA-1:exon:9681;Parent=Msa1355970-mRNA-1
tig0015260 exon 4915 5111 4915 ID=Msa1355970-mRNA-1:exon:9680;Parent=Msa1355970-mRNA-1
tig0015260 five_prime_UTR 4990 5111 4990 ID=Msa1355970-mRNA-1:five_prime_utr;Parent=Msa1355970-mRNA-1
tig0015260 CDS 4915 4989 4915 ID=Msa1355970-mRNA-1:cds;Parent=Msa1355970-mRNA-1
tig0015260 CDS 4750 4812 4750 ID=Msa1355970-mRNA-1:cds;Parent=Msa1355970-mRNA-1
tig0015260 three_prime_UTR 4742 4749 4742 ID=Msa1355970-mRNA-1:three_prime_utr;Parent=Msa1355970-mRNA-1
tig0015260 three_prime_UTR 1638 2200 1638 ID=Msa1355970-mRNA-1:three_prime_utr;Parent=Msa1355970-mRNA-1
Gene Sequence

>Msa1355970

ATGGCGCAAGATCAATACTCACCGGCAGTTCCTTTATTTCTCGTTCTTCTAATAGCAGCTCATGTTCTCGGTCTGGTATTTTGGATTTATAAATTAGCTACTCAGAAACAACCACTGCAGAGGCGAAAGGCTCACTGA
Protein sequence

>Msa1355970

MAQDQYSPAVPLFLVLLIAAHVLGLVFWIYKLATQKQPLQRRKAH*