AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene070242


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070242.t1 MTR_7g029510 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene070242.t1 MTR_7g099610 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene070242.t1 MTR_5g063620 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene070242.t1 MTR_4g061827 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene070242.t1 MTR_4g061920 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene070242.t1 MTR_3g462400 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene070242.t1 MTR_3g054380 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene070242.t1 MTR_3g462730 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene070242.t1 MTR_3g462510 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene070242.t1 MTR_3g070920 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene070242.t1 MTR_3g061440 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene070242.t1 MTR_3g462410 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene070242.t1 MTR_2g096100 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene070242.t1 MTR_8g038460 100.000 103 0 0 1 103 1 103 2.85e-68 199
MS.gene070242.t1 MTR_4g128150 100.000 102 0 0 1 102 1 102 1.67e-66 204
MS.gene070242.t1 MTR_7g029520 84.000 50 8 0 27 76 1 50 2.15e-21 87.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070242.t1 AT5G59970 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene070242.t1 AT5G59690 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene070242.t1 AT5G59970 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene070242.t1 AT3G46320 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene070242.t1 AT3G45930 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene070242.t1 AT3G53730 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene070242.t1 AT2G28740 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene070242.t1 AT1G07660 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene070242.t1 AT1G07820 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene070242.t1 AT1G07820 100.000 103 0 0 1 103 1 103 2.99e-68 199
MS.gene070242.t1 AT1G07660 83.495 103 0 1 1 103 1 86 4.34e-51 155

Find 32 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGCAGGGAAGGACTCTTTA+TGG 0.247348 8.2:+6155719 MS.gene070242:CDS
GGAAGGACTCTTTATGGATT+TGG 0.297351 8.2:+6155725 MS.gene070242:CDS
TCCTTTGCCTCTTCCTGACA+TGG 0.418595 8.2:-6155441 None:intergenic
TCTTTATGGATTTGGCGGTT+AGG 0.439485 8.2:+6155733 MS.gene070242:CDS
GGCAAAGGAGGAAAGGGTCT+TGG 0.446329 8.2:+6155455 MS.gene070242:CDS
CAAGAATTCACAGCCATGTC+AGG 0.481900 8.2:+6155428 None:intergenic
CGTTTACGCTCTCAAGAGGC+AGG 0.488613 8.2:+6155703 MS.gene070242:CDS
GGTTCTTCGCGATAACATCC+AGG 0.500175 8.2:+6155505 MS.gene070242:CDS
AGGACTCTTTATGGATTTGG+CGG 0.509079 8.2:+6155728 MS.gene070242:CDS
GGAGGAAAGGGTCTTGGAAA+GGG 0.511441 8.2:+6155461 MS.gene070242:CDS
TCGCAGGCTTCGTGATTCCC+TGG 0.511861 8.2:-6155523 None:intergenic
AGGAAGAGGCAAAGGAGGAA+AGG 0.518412 8.2:+6155448 MS.gene070242:CDS
GGAAGAGGCAAAGGAGGAAA+GGG 0.527098 8.2:+6155449 MS.gene070242:CDS
CCATCGCAGTAACAGTCTTA+CGG 0.545551 8.2:-6155676 None:intergenic
GCCATGTCAGGAAGAGGCAA+AGG 0.547501 8.2:+6155440 None:intergenic
AGGAGGAAAGGGTCTTGGAA+AGG 0.553664 8.2:+6155460 MS.gene070242:CDS
CCGTAAGACTGTTACTGCGA+TGG 0.561095 8.2:+6155676 MS.gene070242:CDS
CTTGCGAGACGACGAATCGC+AGG 0.571080 8.2:-6155539 None:intergenic
TTCACAGCCATGTCAGGAAG+AGG 0.573281 8.2:+6155434 None:intergenic
AGGAGCAAAACGACACCGTA+AGG 0.575994 8.2:+6155484 MS.gene070242:CDS
TGTTATCGCGAAGAACCTTA+CGG 0.576355 8.2:-6155499 None:intergenic
ATGTCAGGAAGAGGCAAAGG+AGG 0.581057 8.2:+6155443 MS.gene070242:CDS
ACGCTCTCAAGAGGCAGGGA+AGG 0.583359 8.2:+6155708 MS.gene070242:CDS
ACGGCGAGCATGCTCTGTGT+AGG 0.590446 8.2:-6155657 None:intergenic
GGAAAGGGTCTTGGAAAGGG+AGG 0.621886 8.2:+6155464 MS.gene070242:CDS
ATTCGTCGTCTCGCAAGACG+TGG 0.624267 8.2:+6155545 MS.gene070242:CDS
TTAATCTATGAAGAAACTCG+TGG 0.634992 8.2:+6155590 MS.gene070242:CDS
ATGTCGTTTACGCTCTCAAG+AGG 0.636714 8.2:+6155699 MS.gene070242:CDS
CGTCGTCTCGCAAGACGTGG+TGG 0.642630 8.2:+6155548 MS.gene070242:CDS
GTTCTTCGCGATAACATCCA+GGG 0.657095 8.2:+6155506 MS.gene070242:CDS
GTTTACGCTCTCAAGAGGCA+GGG 0.663401 8.2:+6155704 MS.gene070242:CDS
GGTGGTGTGAAGCGTATCAG+TGG 0.669421 8.2:+6155566 MS.gene070242:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TTAATCTATGAAGAAACTCG+TGG + chr8.2:6155590-6155609 MS.gene070242:CDS 30.0%
AGGACTCTTTATGGATTTGG+CGG + chr8.2:6155728-6155747 MS.gene070242:CDS 40.0%
GGAAGGACTCTTTATGGATT+TGG + chr8.2:6155725-6155744 MS.gene070242:CDS 40.0%
TGTTATCGCGAAGAACCTTA+CGG - chr8.2:6155502-6155521 None:intergenic 40.0%
CCATCGCAGTAACAGTCTTA+CGG - chr8.2:6155679-6155698 None:intergenic 45.0%
GTTCTTCGCGATAACATCCA+GGG + chr8.2:6155506-6155525 MS.gene070242:CDS 45.0%
! ATGTCGTTTACGCTCTCAAG+AGG + chr8.2:6155699-6155718 MS.gene070242:CDS 45.0%
AGGAAGAGGCAAAGGAGGAA+AGG + chr8.2:6155448-6155467 MS.gene070242:CDS 50.0%
AGGAGCAAAACGACACCGTA+AGG + chr8.2:6155484-6155503 MS.gene070242:CDS 50.0%
AGGAGGAAAGGGTCTTGGAA+AGG + chr8.2:6155460-6155479 MS.gene070242:CDS 50.0%
AGGCAGGGAAGGACTCTTTA+TGG + chr8.2:6155719-6155738 MS.gene070242:CDS 50.0%
ATGTCAGGAAGAGGCAAAGG+AGG + chr8.2:6155443-6155462 MS.gene070242:CDS 50.0%
CCGTAAGACTGTTACTGCGA+TGG + chr8.2:6155676-6155695 MS.gene070242:CDS 50.0%
GGAAGAGGCAAAGGAGGAAA+GGG + chr8.2:6155449-6155468 MS.gene070242:CDS 50.0%
GGAGGAAAGGGTCTTGGAAA+GGG + chr8.2:6155461-6155480 MS.gene070242:CDS 50.0%
GGTTCTTCGCGATAACATCC+AGG + chr8.2:6155505-6155524 MS.gene070242:CDS 50.0%
GTTTACGCTCTCAAGAGGCA+GGG + chr8.2:6155704-6155723 MS.gene070242:CDS 50.0%
ATTCGTCGTCTCGCAAGACG+TGG + chr8.2:6155545-6155564 MS.gene070242:CDS 55.0%
CGTTTACGCTCTCAAGAGGC+AGG + chr8.2:6155703-6155722 MS.gene070242:CDS 55.0%
GGAAAGGGTCTTGGAAAGGG+AGG + chr8.2:6155464-6155483 MS.gene070242:CDS 55.0%
GGCAAAGGAGGAAAGGGTCT+TGG + chr8.2:6155455-6155474 MS.gene070242:CDS 55.0%
GGTGGTGTGAAGCGTATCAG+TGG + chr8.2:6155566-6155585 MS.gene070242:CDS 55.0%
ACGCTCTCAAGAGGCAGGGA+AGG + chr8.2:6155708-6155727 MS.gene070242:CDS 60.0%
ACGGCGAGCATGCTCTGTGT+AGG - chr8.2:6155660-6155679 None:intergenic 60.0%
CTTGCGAGACGACGAATCGC+AGG - chr8.2:6155542-6155561 None:intergenic 60.0%
TCGCAGGCTTCGTGATTCCC+TGG - chr8.2:6155526-6155545 None:intergenic 60.0%
CGTCGTCTCGCAAGACGTGG+TGG + chr8.2:6155548-6155567 MS.gene070242:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 6155443 6155754 6155443 ID=MS.gene070242
chr8.2 mRNA 6155443 6155754 6155443 ID=MS.gene070242.t1;Parent=MS.gene070242
chr8.2 exon 6155443 6155754 6155443 ID=MS.gene070242.t1.exon1;Parent=MS.gene070242.t1
chr8.2 CDS 6155443 6155754 6155443 ID=cds.MS.gene070242.t1;Parent=MS.gene070242.t1
Gene Sequence

>MS.gene070242

ATGTCAGGAAGAGGCAAAGGAGGAAAGGGTCTTGGAAAGGGAGGAGCAAAACGACACCGTAAGGTTCTTCGCGATAACATCCAGGGAATCACGAAGCCTGCGATTCGTCGTCTCGCAAGACGTGGTGGTGTGAAGCGTATCAGTGGCTTAATCTATGAAGAAACTCGTGGTGTTCTCAAAATCTTTCTTGAGAATGTGATTCGTGATGCTGTCACCTACACAGAGCATGCTCGCCGTAAGACTGTTACTGCGATGGATGTCGTTTACGCTCTCAAGAGGCAGGGAAGGACTCTTTATGGATTTGGCGGTTAG

Protein sequence

>MS.gene070242.t1

MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKIFLENVIRDAVTYTEHARRKTVTAMDVVYALKRQGRTLYGFGG