AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene46487


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene46487.t1 MTR_1g054305 99.242 132 1 0 1 132 228 359 5.37e-88 261
MS.gene46487.t1 MTR_4g127800 29.197 137 86 2 2 132 248 379 6.54e-13 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene46487.t1 AT3G11945 80.000 130 26 0 1 130 222 351 4.33e-68 210
MS.gene46487.t1 AT3G11945 80.000 130 26 0 1 130 229 358 6.60e-68 210
MS.gene46487.t1 AT2G18950 31.496 127 86 1 2 127 230 356 3.19e-15 71.2
MS.gene46487.t1 AT2G18950 33.708 89 58 1 2 89 230 318 6.91e-12 61.2

Find 22 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGACCTTTGCTACAAAATT+AGG 0.197774 1.1:-42142075 MS.gene46487:CDS
GGTGTTGTTGACAGGCTTTC+AGG 0.223817 1.1:-42141891 MS.gene46487:intron
AACATTTCTTTCCTTGGTTC+TGG 0.229462 1.1:-42142045 MS.gene46487:CDS
CATATAAATTGCTGCCAATA+TGG 0.315997 1.1:+42141996 None:intergenic
GTTCGGAACATTTCTTTCCT+TGG 0.385731 1.1:-42142051 MS.gene46487:CDS
GAACTATGTTGTTACCATAT+TGG 0.406125 1.1:-42142010 MS.gene46487:CDS
ATAATAAGAAATGCTGCAAC+AGG 0.417997 1.1:+42142815 None:intergenic
TTGCTACAAAATTAGGAGTT+CGG 0.428806 1.1:-42142068 MS.gene46487:CDS
CTACGATCTCCTTCAACATC+TGG 0.429352 1.1:+42142310 None:intergenic
GTTGACAGGCTTTCAGGAGA+TGG 0.469985 1.1:-42141885 MS.gene46487:intron
AAAGATCTTCCAGATGTTGA+AGG 0.481104 1.1:-42142319 MS.gene46487:CDS
AGCATTTCTTATTATTGCTA+CGG 0.484068 1.1:-42142807 MS.gene46487:intron
TGCTTCAAGCTTGATTTACC+AGG 0.498689 1.1:-42141836 MS.gene46487:CDS
GGCAGCAATTTATATGCCCC+AGG 0.523927 1.1:-42141989 MS.gene46487:intron
ATTAATCCGCTCATCGAACC+TGG 0.545003 1.1:+42141818 None:intergenic
TACGCCACTAGAGCTGCTCT+TGG 0.549187 1.1:-42142639 MS.gene46487:CDS
AAGTCCAAGAGCAGCTCTAG+TGG 0.553924 1.1:+42142635 None:intergenic
ATCTGCCTGCAGCTCTCCTG+TGG 0.560779 1.1:-42142392 MS.gene46487:intron
GAAGCAAATATTGCATGAGC+TGG 0.584720 1.1:+42141853 None:intergenic
TCAGCAAAATTCCAGAACCA+AGG 0.633793 1.1:+42142034 None:intergenic
CAGATGTTGAAGGAGATCGT+AGG 0.666387 1.1:-42142309 MS.gene46487:intron
AAGAGACTTATGCAGGTACG+CGG 0.681026 1.1:-42142687 MS.gene46487:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAGGTTTATTTTGAAATGA+AGG + chr1.1:42142734-42142753 None:intergenic 20.0%
! AATAAACCTTATGTAAGTTG+TGG - chr1.1:42142743-42142762 MS.gene46487:intron 25.0%
! AGCATTTCTTATTATTGCTA+CGG - chr1.1:42141848-42141867 MS.gene46487:CDS 25.0%
! ATTGCATGTAATAATTAACC+AGG - chr1.1:42142548-42142567 MS.gene46487:intron 25.0%
!! ACAGTTAGTAAAGTATCATT+TGG - chr1.1:42141928-42141947 MS.gene46487:intron 25.0%
!!! GTTGTTTCATATTTTGCAAT+TGG - chr1.1:42142077-42142096 MS.gene46487:CDS 25.0%
AAAGTTGTGATAAAAACCAC+AGG + chr1.1:42142282-42142301 None:intergenic 30.0%
AGTGTCTATTGTTTGATTAG+AGG - chr1.1:42142126-42142145 MS.gene46487:intron 30.0%
ATAATAAGAAATGCTGCAAC+AGG + chr1.1:42141843-42141862 None:intergenic 30.0%
CATATAAATTGCTGCCAATA+TGG + chr1.1:42142662-42142681 None:intergenic 30.0%
GAACTATGTTGTTACCATAT+TGG - chr1.1:42142645-42142664 MS.gene46487:CDS 30.0%
GATCATCAACAAATAAAGAG+TGG + chr1.1:42142513-42142532 None:intergenic 30.0%
TGATGATCATGCATGTAATA+TGG - chr1.1:42142525-42142544 MS.gene46487:intron 30.0%
TTAGAATCATGTTGCGTTAA+GGG - chr1.1:42142375-42142394 MS.gene46487:CDS 30.0%
TTGCTACAAAATTAGGAGTT+CGG - chr1.1:42142587-42142606 MS.gene46487:intron 30.0%
! AACTCCTAATTTTGTAGCAA+AGG + chr1.1:42142587-42142606 None:intergenic 30.0%
! TTTAGAATCATGTTGCGTTA+AGG - chr1.1:42142374-42142393 MS.gene46487:CDS 30.0%
! TTTCGTAACATTTTTCGCAT+TGG - chr1.1:42142299-42142318 MS.gene46487:intron 30.0%
AAAGATCTTCCAGATGTTGA+AGG - chr1.1:42142336-42142355 MS.gene46487:CDS 35.0%
AACATTTCTTTCCTTGGTTC+TGG - chr1.1:42142610-42142629 MS.gene46487:intron 35.0%
ACAACACCACAACTTACATA+AGG + chr1.1:42142752-42142771 None:intergenic 35.0%
ACAAGATCAAACGCATAAGT+TGG + chr1.1:42142241-42142260 None:intergenic 35.0%
AGTGCAAAGATTGTGAGAAA+AGG + chr1.1:42141887-42141906 None:intergenic 35.0%
TCGACCTTTGCTACAAAATT+AGG - chr1.1:42142580-42142599 MS.gene46487:intron 35.0%
! AGACTAGTTAACATTCACCT+GGG + chr1.1:42142686-42142705 None:intergenic 35.0%
AAAGGTCGATATCTGATACC+TGG + chr1.1:42142569-42142588 None:intergenic 40.0%
GAAGCAAATATTGCATGAGC+TGG + chr1.1:42142805-42142824 None:intergenic 40.0%
GCAAAGATTGTGAGAAAAGG+AGG + chr1.1:42141884-42141903 None:intergenic 40.0%
GTTAAGGGTTGCAAGTTCAT+TGG - chr1.1:42142390-42142409 MS.gene46487:intron 40.0%
GTTCGGAACATTTCTTTCCT+TGG - chr1.1:42142604-42142623 MS.gene46487:intron 40.0%
TCAGCAAAATTCCAGAACCA+AGG + chr1.1:42142624-42142643 None:intergenic 40.0%
TGCTTCAAGCTTGATTTACC+AGG - chr1.1:42142819-42142838 MS.gene46487:CDS 40.0%
TTGACAGAAGAGACTTATGC+AGG - chr1.1:42141961-42141980 MS.gene46487:intron 40.0%
! AACGCATAAGTTGGAGACAA+CGG + chr1.1:42142232-42142251 None:intergenic 40.0%
! CGCGGTTTTCTACTAAACTT+TGG - chr1.1:42141986-42142005 MS.gene46487:intron 40.0%
! GACTAGTTAACATTCACCTG+GGG + chr1.1:42142685-42142704 None:intergenic 40.0%
! GAGACTAGTTAACATTCACC+TGG + chr1.1:42142687-42142706 None:intergenic 40.0%
!! TAAGTTGTGGTGTTGTTGAC+AGG - chr1.1:42142756-42142775 MS.gene46487:intron 40.0%
AAGAGACTTATGCAGGTACG+CGG - chr1.1:42141968-42141987 MS.gene46487:intron 45.0%
CAGATGTTGAAGGAGATCGT+AGG - chr1.1:42142346-42142365 MS.gene46487:CDS 45.0%
CTACGATCTCCTTCAACATC+TGG + chr1.1:42142348-42142367 None:intergenic 45.0%
!! CTCTTGGACTTGCTTTTGAG+TGG - chr1.1:42142032-42142051 MS.gene46487:CDS 45.0%
!! TTGGACTTGCTTTTGAGTGG+AGG - chr1.1:42142035-42142054 MS.gene46487:CDS 45.0%
AAAAACCACAGGAGAGCTGC+AGG + chr1.1:42142271-42142290 None:intergenic 50.0%
GGCAGCAATTTATATGCCCC+AGG - chr1.1:42142666-42142685 MS.gene46487:CDS 50.0%
GTTGACAGGCTTTCAGGAGA+TGG - chr1.1:42142770-42142789 MS.gene46487:intron 50.0%
! AAGTCCAAGAGCAGCTCTAG+TGG + chr1.1:42142023-42142042 None:intergenic 50.0%
!! GGTGTTGTTGACAGGCTTTC+AGG - chr1.1:42142764-42142783 MS.gene46487:intron 50.0%
TACGCCACTAGAGCTGCTCT+TGG - chr1.1:42142016-42142035 MS.gene46487:CDS 55.0%
ATCTGCCTGCAGCTCTCCTG+TGG - chr1.1:42142263-42142282 MS.gene46487:intron 60.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 42141828 42142849 42141828 ID=MS.gene46487
chr1.1 mRNA 42141828 42142849 42141828 ID=MS.gene46487.t1;Parent=MS.gene46487
chr1.1 exon 42142808 42142849 42142808 ID=MS.gene46487.t1.exon1;Parent=MS.gene46487.t1
chr1.1 CDS 42142808 42142849 42142808 ID=cds.MS.gene46487.t1;Parent=MS.gene46487.t1
chr1.1 exon 42142621 42142694 42142621 ID=MS.gene46487.t1.exon2;Parent=MS.gene46487.t1
chr1.1 CDS 42142621 42142694 42142621 ID=cds.MS.gene46487.t1;Parent=MS.gene46487.t1
chr1.1 exon 42142310 42142402 42142310 ID=MS.gene46487.t1.exon3;Parent=MS.gene46487.t1
chr1.1 CDS 42142310 42142402 42142310 ID=cds.MS.gene46487.t1;Parent=MS.gene46487.t1
chr1.1 exon 42141990 42142107 42141990 ID=MS.gene46487.t1.exon4;Parent=MS.gene46487.t1
chr1.1 CDS 42141990 42142107 42141990 ID=cds.MS.gene46487.t1;Parent=MS.gene46487.t1
chr1.1 exon 42141828 42141899 42141828 ID=MS.gene46487.t1.exon5;Parent=MS.gene46487.t1
chr1.1 CDS 42141828 42141899 42141828 ID=cds.MS.gene46487.t1;Parent=MS.gene46487.t1
Gene Sequence

>MS.gene46487

ATGAAACGCTTTCCTGTTGCAGCATTTCTTATTATTGCTACGGTATGACATAACCTCCTTTTCTCACAATCTTTGCACTGCTAACTGTAATTTGTCAATTACAGTTAGTAAAGTATCATTTGGATGAACAAGATTGACAGAAGAGACTTATGCAGGTACGCGGTTTTCTACTAAACTTTGGTGTGTACTACGCCACTAGAGCTGCTCTTGGACTTGCTTTTGAGTGGAGGTGAGTTAGAGCTTATCGTTGTTGTTTCATATTTTGCAATTGGTAAAGTTTTGAACAACTGAGATCGCAAGTGTCTATTGTTTGATTAGAGGCTCAGCAATGCTTATATTGACTATGAATTATGTTGCTGCTATTATAATGAATAAAACTTGTGATGATGTGAGATTTTCATCCGTTGTCTCCAACTTATGCGTTTGATCTTGTATATCTGCCTGCAGCTCTCCTGTGGTTTTTATCACAACTTTCGTAACATTTTTCGCATTGGTAATTGCTATAACAAAAGATCTTCCAGATGTTGAAGGAGATCGTAGGTACGTTTTAGAATCATGTTGCGTTAAGGGTTGCAAGTTCATTGGTTCTATTTATCTGCGATGATGATACATCATTTGACTTATATTTCATTATATACATGCGCGCGCGCATGCTTGCATCTTACATAATCTGCTACTGCTACCACTCTTTATTTGTTGATGATCATGCATGTAATATGGATTGCATGTAATAATTAACCAGGTATCAGATATCGACCTTTGCTACAAAATTAGGAGTTCGGAACATTTCTTTCCTTGGTTCTGGAATTTTGCTGATGAACTATGTTGTTACCATATTGGCAGCAATTTATATGCCCCAGGTGAATGTTAACTAGTCTCATGTTCGAATCTCTTATCATTTGTCCTTCATTTCAAAATAAACCTTATGTAAGTTGTGGTGTTGTTGACAGGCTTTCAGGAGATGGTTACTGATACCAGCTCATGCAATATTTGCTTCAAGCTTGATTTACCAGGTTCGATGA

Protein sequence

>MS.gene46487.t1

MKRFPVAAFLIIATVRGFLLNFGVYYATRAALGLAFEWSSPVVFITTFVTFFALVIAITKDLPDVEGDRRYQISTFATKLGVRNISFLGSGILLMNYVVTILAAIYMPQAFRRWLLIPAHAIFASSLIYQVR