AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene77196


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77196.t1 MTR_7g059255 98.765 81 1 0 6 86 94 174 3.03e-53 163
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77196.t1 AT4G13220 60.976 82 30 1 7 86 95 176 4.64e-29 102

Find 24 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTACAGCTTTCTTTATTT+TGG 0.099548 7.2:-37782146 MS.gene77196:intron
GTTACTGAAATCCTTGCTAT+TGG 0.328579 7.2:+37782214 None:intergenic
ATCCTTTGGAAGAGGCTAAT+AGG 0.388720 7.2:-37781320 MS.gene77196:CDS
CTTGATCTTTATTGAATGCT+AGG 0.393502 7.2:+37781388 None:intergenic
GAAATCCTTGCTATTGGTCT+TGG 0.422289 7.2:+37782220 None:intergenic
CAAAGGATCATCCATAGGTT+TGG 0.442636 7.2:+37781335 None:intergenic
CAAACCTATGGATGATCCTT+TGG 0.442745 7.2:-37781334 MS.gene77196:CDS
GGAAGAGGCTAATAGGATAA+TGG 0.446378 7.2:-37781313 MS.gene77196:CDS
CAAGAACCACCTAGCGGGAA+TGG 0.467436 7.2:-37781369 MS.gene77196:CDS
GTTTGTTCATAGGCTACCAT+AGG 0.522734 7.2:-37781429 MS.gene77196:intron
CTGCAGAATGCATGGAGAGT+TGG 0.536539 7.2:-37782588 None:intergenic
AGGAACTACGACCAAACCTA+TGG 0.545163 7.2:-37781346 MS.gene77196:CDS
TGCAAAGTATGCAAGTCCTA+TGG 0.552339 7.2:+37781413 None:intergenic
AGATAGTTCTGCAGAATGCA+TGG 0.555735 7.2:-37782596 None:intergenic
AAGATCAAGAACCACCTAGC+GGG 0.560815 7.2:-37781374 MS.gene77196:CDS
ATCCTATTAGCCTCTTCCAA+AGG 0.564567 7.2:+37781318 None:intergenic
TATGGATGATCCTTTGGAAG+AGG 0.564870 7.2:-37781328 MS.gene77196:CDS
GTAGTTCCTCCATTCCCGCT+AGG 0.572693 7.2:+37781360 None:intergenic
TCTTCCAAAGGATCATCCAT+AGG 0.581655 7.2:+37781330 None:intergenic
AAATAAAGAAAGCTGTAGAG+AGG 0.616626 7.2:+37782149 None:intergenic
GTTCCTCCATTCCCGCTAGG+TGG 0.621366 7.2:+37781363 None:intergenic
GAACCACCTAGCGGGAATGG+AGG 0.633606 7.2:-37781366 MS.gene77196:CDS
CTGTACCAAGACCAATAGCA+AGG 0.633937 7.2:-37782225 MS.gene77196:CDS
AAAGATCAAGAACCACCTAG+CGG 0.725332 7.2:-37781375 MS.gene77196:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTAATATGATATGAAAAA+TGG + chr7.2:37781768-37781787 None:intergenic 10.0%
!! AATAGTTGAGACTTATTTAT+AGG + chr7.2:37781927-37781946 None:intergenic 20.0%
!! ATAGTTGAGACTTATTTATA+GGG + chr7.2:37781926-37781945 None:intergenic 20.0%
!! TTCCCTTTAATAACATAATA+TGG + chr7.2:37782121-37782140 None:intergenic 20.0%
! AAAAAATTCAACCCTACAAA+AGG + chr7.2:37782173-37782192 None:intergenic 25.0%
! AAAAATTCAACCCTACAAAA+GGG + chr7.2:37782172-37782191 None:intergenic 25.0%
! ATAGTGAATAAACCATAGTT+AGG - chr7.2:37781838-37781857 MS.gene77196:intron 25.0%
! GGCCATATTATGTTATTAAA+GGG - chr7.2:37782116-37782135 MS.gene77196:intron 25.0%
! GTAAAAAACTTGAAAACAAC+AGG + chr7.2:37781566-37781585 None:intergenic 25.0%
! GTTAGGTTATATGTAAACTA+TGG - chr7.2:37781855-37781874 MS.gene77196:intron 25.0%
! GTTTACATATAACCTAACTA+TGG + chr7.2:37781853-37781872 None:intergenic 25.0%
! TAGTTGAGACTTATTTATAG+GGG + chr7.2:37781925-37781944 None:intergenic 25.0%
! TGGCCATATTATGTTATTAA+AGG - chr7.2:37782115-37782134 MS.gene77196:intron 25.0%
! TTGTACTAAATCACTTATTG+CGG + chr7.2:37781442-37781461 None:intergenic 25.0%
!! AACATCAATGCTATATTTTG+TGG - chr7.2:37782412-37782431 MS.gene77196:intron 25.0%
!!! TACAAAGTATTGTCAAACAA+CGG - chr7.2:37782275-37782294 MS.gene77196:intron 25.0%
AAATAAAGAAAGCTGTAGAG+AGG + chr7.2:37781734-37781753 None:intergenic 30.0%
AAATGGTAATTGTGACTTAC+AGG + chr7.2:37781352-37781371 None:intergenic 30.0%
AATGGTAATTGTGACTTACA+GGG + chr7.2:37781351-37781370 None:intergenic 30.0%
ATATTTGTGCTATATGGTAG+CGG - chr7.2:37781393-37781412 MS.gene77196:CDS 30.0%
ATCCTAAAGTCTTAACCATT+GGG + chr7.2:37782378-37782397 None:intergenic 30.0%
CAAGTACATTGTTTGTTCAT+AGG - chr7.2:37782441-37782460 MS.gene77196:intron 30.0%
CTTGATCTTTATTGAATGCT+AGG + chr7.2:37782495-37782514 None:intergenic 30.0%
GAGAAACATGAAAAACTAGA+AGG + chr7.2:37781602-37781621 None:intergenic 30.0%
TAAGTCACAATTACCATTTC+CGG - chr7.2:37781353-37781372 MS.gene77196:CDS 30.0%
TAGCAGATATTTGTGCTATA+TGG - chr7.2:37781387-37781406 MS.gene77196:CDS 30.0%
TATCCTAAAGTCTTAACCAT+TGG + chr7.2:37782379-37782398 None:intergenic 30.0%
TGGTTTCATGTTATGAATCA+TGG + chr7.2:37782073-37782092 None:intergenic 30.0%
! AAGGATTTCAGTAACTTTTG+TGG - chr7.2:37781674-37781693 MS.gene77196:intron 30.0%
! ATATGTCTTTTCCCTTTTGT+AGG - chr7.2:37782158-37782177 MS.gene77196:CDS 30.0%
! CTATGGTTAGTTAGTTAGTT+AGG - chr7.2:37781872-37781891 MS.gene77196:intron 30.0%
! TATGTCTTTTCCCTTTTGTA+GGG - chr7.2:37782159-37782178 MS.gene77196:CDS 30.0%
!! CTCTACAGCTTTCTTTATTT+TGG - chr7.2:37781734-37781753 MS.gene77196:intron 30.0%
AAGTCACAATTACCATTTCC+GGG - chr7.2:37781354-37781373 MS.gene77196:CDS 35.0%
ACATGAATGAACATGGCAAT+TGG + chr7.2:37782093-37782112 None:intergenic 35.0%
ACTCTACTATGAGTCACTAT+GGG - chr7.2:37781980-37781999 MS.gene77196:intron 35.0%
ATAGCATAGGTGTATTCATG+TGG + chr7.2:37782012-37782031 None:intergenic 35.0%
ATCAGCAAGAAAGATAGCAT+AGG + chr7.2:37782025-37782044 None:intergenic 35.0%
ATCATTATGACCTTTATCCC+TGG - chr7.2:37782043-37782062 MS.gene77196:intron 35.0%
ATTGACAACAAGTTTCCCAA+TGG - chr7.2:37782360-37782379 MS.gene77196:intron 35.0%
TACTCTACTATGAGTCACTA+TGG - chr7.2:37781979-37781998 MS.gene77196:intron 35.0%
TAGCATAGGTGTATTCATGT+GGG + chr7.2:37782011-37782030 None:intergenic 35.0%
TCATTATGACCTTTATCCCT+GGG - chr7.2:37782044-37782063 MS.gene77196:intron 35.0%
TCTAGCATCAGCAAAAAGAA+GGG + chr7.2:37781632-37781651 None:intergenic 35.0%
TTATGAATCATGGAATCCCA+GGG + chr7.2:37782063-37782082 None:intergenic 35.0%
TTCCCAATGGTTAAGACTTT+AGG - chr7.2:37782373-37782392 MS.gene77196:intron 35.0%
TTCTAGCATCAGCAAAAAGA+AGG + chr7.2:37781633-37781652 None:intergenic 35.0%
! GTTACTGAAATCCTTGCTAT+TGG + chr7.2:37781669-37781688 None:intergenic 35.0%
! TACAAAGTCTTGTCGAATAG+TGG - chr7.2:37781457-37781476 MS.gene77196:intron 35.0%
!! GGGAAACTTGTTGTCAATTA+CGG + chr7.2:37782358-37782377 None:intergenic 35.0%
AAAGATCAAGAACCACCTAG+CGG - chr7.2:37782505-37782524 MS.gene77196:intron 40.0%
ATCACTTATTGCGGAACAAC+AGG + chr7.2:37781433-37781452 None:intergenic 40.0%
CAAACCTATGGATGATCCTT+TGG - chr7.2:37782546-37782565 MS.gene77196:intron 40.0%
CAAAGGATCATCCATAGGTT+TGG + chr7.2:37782548-37782567 None:intergenic 40.0%
GGAAGAGGCTAATAGGATAA+TGG - chr7.2:37782567-37782586 MS.gene77196:intron 40.0%
GTTATGAATCATGGAATCCC+AGG + chr7.2:37782064-37782083 None:intergenic 40.0%
GTTTGTTCATAGGCTACCAT+AGG - chr7.2:37782451-37782470 MS.gene77196:intron 40.0%
TATTTGACAATGCCCGGAAA+TGG + chr7.2:37781369-37781388 None:intergenic 40.0%
TCGAATAGTGGCTATAACAG+TGG - chr7.2:37781469-37781488 MS.gene77196:intron 40.0%
TCTTCCAAAGGATCATCCAT+AGG + chr7.2:37782553-37782572 None:intergenic 40.0%
TGCAAAGTATGCAAGTCCTA+TGG + chr7.2:37782470-37782489 None:intergenic 40.0%
TGCCATGTTCATTCATGTAC+TGG - chr7.2:37782095-37782114 MS.gene77196:intron 40.0%
! ATAGCTGCTGTTTGAAACAG+TGG + chr7.2:37782206-37782225 None:intergenic 40.0%
! ATCCTATTAGCCTCTTCCAA+AGG + chr7.2:37782565-37782584 None:intergenic 40.0%
! ATCCTTTGGAAGAGGCTAAT+AGG - chr7.2:37782560-37782579 MS.gene77196:intron 40.0%
! ATCTGCTATTTGACAATGCC+CGG + chr7.2:37781375-37781394 None:intergenic 40.0%
! TATGGATGATCCTTTGGAAG+AGG - chr7.2:37782552-37782571 MS.gene77196:intron 40.0%
!! GAAATCCTTGCTATTGGTCT+TGG + chr7.2:37781663-37781682 None:intergenic 40.0%
!!! ACAGGCAGTTTTTTCAATCG+CGG + chr7.2:37781548-37781567 None:intergenic 40.0%
AAGATCAAGAACCACCTAGC+GGG - chr7.2:37782506-37782525 MS.gene77196:intron 45.0%
AGGAACTACGACCAAACCTA+TGG - chr7.2:37782534-37782553 MS.gene77196:intron 45.0%
CAATCGCGGATCACAGAAAA+TGG + chr7.2:37781534-37781553 None:intergenic 45.0%
CTGTACCAAGACCAATAGCA+AGG - chr7.2:37781655-37781674 MS.gene77196:intron 45.0%
GGCCAGTACATGAATGAACA+TGG + chr7.2:37782100-37782119 None:intergenic 45.0%
GGTAAGGATGGAATTGATGC+AGG - chr7.2:37781313-37781332 MS.gene77196:CDS 45.0%
GTTATAGCACTGCAGTGTAG+CGG - chr7.2:37781493-37781512 MS.gene77196:intron 45.0%
TCATGGAATCCCAGGGATAA+AGG + chr7.2:37782056-37782075 None:intergenic 45.0%
!! GCATGGAGAGTTGGTAAGGA+TGG - chr7.2:37781301-37781320 MS.gene77196:CDS 50.0%
CAAGAACCACCTAGCGGGAA+TGG - chr7.2:37782511-37782530 MS.gene77196:intron 55.0%
GTAGTTCCTCCATTCCCGCT+AGG + chr7.2:37782523-37782542 None:intergenic 55.0%
GAACCACCTAGCGGGAATGG+AGG - chr7.2:37782514-37782533 MS.gene77196:intron 60.0%
GTTCCTCCATTCCCGCTAGG+TGG + chr7.2:37782520-37782539 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 37781299 37782603 37781299 ID=MS.gene77196
chr7.2 mRNA 37781299 37782603 37781299 ID=MS.gene77196.t1;Parent=MS.gene77196
chr7.2 exon 37782589 37782603 37782589 ID=MS.gene77196.t1.exon1;Parent=MS.gene77196.t1
chr7.2 CDS 37782589 37782603 37782589 ID=cds.MS.gene77196.t1;Parent=MS.gene77196.t1
chr7.2 exon 37782147 37782251 37782147 ID=MS.gene77196.t1.exon2;Parent=MS.gene77196.t1
chr7.2 CDS 37782147 37782251 37782147 ID=cds.MS.gene77196.t1;Parent=MS.gene77196.t1
chr7.2 exon 37781299 37781439 37781299 ID=MS.gene77196.t1.exon3;Parent=MS.gene77196.t1
chr7.2 CDS 37781299 37781439 37781299 ID=cds.MS.gene77196.t1;Parent=MS.gene77196.t1
Gene Sequence

>MS.gene77196

ATGCATGGAGAGTTGGTAAGGATGGAATTGATGCAGGCACAAATCTAGTCCCTGTAAGTCACAATTACCATTTCCGGGCATTGTCAAATAGCAGATATTTGTGCTATATGGTAGCGGAATTTTAACAAATTCCTGTTGTTCCGCAATAAGTGATTTAGTACAAAGTCTTGTCGAATAGTGGCTATAACAGTGGCGTTATAGCACTGCAGTGTAGCGGAATTTGAACAAATCGCCATTTTCTGTGATCCGCGATTGAAAAAACTGCCTGTTGTTTTCAAGTTTTTTACAAGTACTAGAATACCTTCTAGTTTTTCATGTTTCTCTTTTCTTCCCTTCTTTTTGCTGATGCTAGAATTCTGTACCAAGACCAATAGCAAGGATTTCAGTAACTTTTGTGGCTCTGAGTGCTTTGCTTTTTGTGTTCAAGTCTCTCCTCTCTACAGCTTTCTTTATTTTGGTAAGTCATCCATTTTTCATATCATATTAAAATATCAGATTATTGTTTTAAAATTTTGTATTGCTATAGTATATATTAGAGTATAGTGAATAAACCATAGTTAGGTTATATGTAAACTATGGTTAGTTAGTTAGTTAGGTAGTTGTGAGTTAGTTAGACTAACTCACCCCTATAAATAAGTCTCAACTATTGTACTATCTTTCATCTTTTATCAAATCTAGTTTACTCTACTATGAGTCACTATGGGTATCTCCCACATGAATACACCTATGCTATCTTTCTTGCTGATCATTATGACCTTTATCCCTGGGATTCCATGATTCATAACATGAAACCAATTGCCATGTTCATTCATGTACTGGCCATATTATGTTATTAAAGGGAACTTGTGCATCGAAAAGAATATGTCTTTTCCCTTTTGTAGGGTTGAATTTTTTTTACTTAAAGCCACTGTTTCAAACAGCAGCTATGATTATAGCATAATTTGAACAAATCACTATTCTGCAATACGCTATTTAATACAAAGTATTGTCAAACAACGGCTATAGTTGTGCACTATAGTGTCTCAAAAATTTGAACAAATCACTAATTTCTGCAATCCGTAATTGACAACAAGTTTCCCAATGGTTAAGACTTTAGGATAGTTCTAAGCTTTAAACATCAATGCTATATTTTGTGGTTAACACAAGTACATTGTTTGTTCATAGGCTACCATAGGACTTGCATACTTTGCATACCTAGCATTCAATAAAGATCAAGAACCACCTAGCGGGAATGGAGGAACTACGACCAAACCTATGGATGATCCTTTGGAAGAGGCTAATAGGATAATGGATAAATACAAGTAG

Protein sequence

>MS.gene77196.t1

MHGELNSVPRPIARISVTFVALSALLFVFKSLLSTAFFILATIGLAYFAYLAFNKDQEPPSGNGGTTTKPMDDPLEEANRIMDKYK