AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780038198.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780038198.01.T01 MTR_8g056780 100.000 62 0 0 1 62 1 62 4.46e-36 115
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780038198.01.T01 ATCG00300 96.774 62 2 0 1 62 1 62 5.01e-35 112

Find 9 sgRNAs with CRISPR-Local

Find 12 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGTACACCAATCAATAAAA+TGG 0.272913 7:-41948957 MsG0780038197.01.T01:intron
GTTGTATTTGCTTCTCCTGA+TGG 0.417220 7:+41948980 MsG0780038198.01.T01:CDS
TATTTGCTTCTCCTGATGGT+TGG 0.468755 7:+41948984 MsG0780038198.01.T01:CDS
TCCGGTACATCATTATGGAT+TGG 0.474883 7:+41949031 MsG0780038198.01.T01:CDS
TTATTACTTGACCAACCATC+AGG 0.487376 7:-41948995 MsG0780038197.01.T01:intron
TCCAATCCATAATGATGTAC+CGG 0.492547 7:-41949032 MsG0780038197.01.T01:intron
TCAGGAGAAGCAAATACAAC+AGG 0.517061 7:-41948977 MsG0780038197.01.T01:intron
ATTGGATTAGTATTTCTAGT+GGG 0.580331 7:+41949049 MsG0780038198.01.T01:CDS
GATTGGATTAGTATTTCTAG+TGG 0.595754 7:+41949048 MsG0780038198.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATAAAAATGTTGTATTTTC+CGG + Chr7:41949013-41949032 MsG0780038198.01.T01:CDS 15.0%
!! ACTTCATCCATTTTATTGAT+TGG + Chr7:41948950-41948969 MsG0780038198.01.T01:CDS 25.0%
!!! ATTGGATTAGTATTTCTAGT+GGG + Chr7:41949049-41949068 MsG0780038198.01.T01:CDS 25.0%
AGGTACACCAATCAATAAAA+TGG - Chr7:41948960-41948979 MsG0780038197.01.T01:intron 30.0%
! TATTTTCCGGTACATCATTA+TGG + Chr7:41949026-41949045 MsG0780038198.01.T01:CDS 30.0%
!! GATTGGATTAGTATTTCTAG+TGG + Chr7:41949048-41949067 MsG0780038198.01.T01:CDS 30.0%
TCCAATCCATAATGATGTAC+CGG - Chr7:41949035-41949054 MsG0780038197.01.T01:intron 35.0%
TTATTACTTGACCAACCATC+AGG - Chr7:41948998-41949017 MsG0780038197.01.T01:intron 35.0%
GTTGTATTTGCTTCTCCTGA+TGG + Chr7:41948980-41948999 MsG0780038198.01.T01:CDS 40.0%
TATTTGCTTCTCCTGATGGT+TGG + Chr7:41948984-41949003 MsG0780038198.01.T01:CDS 40.0%
TCAGGAGAAGCAAATACAAC+AGG - Chr7:41948980-41948999 MsG0780038197.01.T01:intron 40.0%
TCCGGTACATCATTATGGAT+TGG + Chr7:41949031-41949050 MsG0780038198.01.T01:CDS 40.0%
Chromosome Type Strat End Strand Name
Chr7 gene 41948908 41949096 41948908 ID=MsG0780038198.01;Name=MsG0780038198.01
Chr7 mRNA 41948908 41949096 41948908 ID=MsG0780038198.01.T01;Parent=MsG0780038198.01;Name=MsG0780038198.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|62
Chr7 exon 41948908 41949096 41948908 ID=MsG0780038198.01.T01:exon:14204;Parent=MsG0780038198.01.T01
Chr7 CDS 41948908 41949096 41948908 ID=MsG0780038198.01.T01:cds;Parent=MsG0780038198.01.T01
Gene Sequence

>MsG0780038198.01.T01

ATGACTATTGCTTTTCAATTAGCTGTTTTTGCATTAATTGTTACTTCATCCATTTTATTGATTGGTGTACCTGTTGTATTTGCTTCTCCTGATGGTTGGTCAAGTAATAAAAATGTTGTATTTTCCGGTACATCATTATGGATTGGATTAGTATTTCTAGTGGGTATTCTTAATTCTCTCATCTCTTGA

Protein sequence

>MsG0780038198.01.T01

MTIAFQLAVFALIVTSSILLIGVPVVFASPDGWSSNKNVVFSGTSLWIGLVFLVGILNSLIS*