AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880043648.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043648.01.T01 MTR_8g039090 95.833 192 8 0 1 192 1 192 4.29e-137 381
MsG0880043648.01.T01 MTR_3g019160 76.316 190 45 0 3 192 2 191 3.75e-109 310
MsG0880043648.01.T01 MTR_3g019090 72.105 190 53 0 3 192 2 191 1.28e-105 301
MsG0880043648.01.T01 MTR_3g019150 72.632 190 51 1 3 192 2 190 6.37e-102 292
MsG0880043648.01.T01 MTR_3g019120 62.827 191 68 1 3 190 2 192 2.90e-86 259
MsG0880043648.01.T01 MTR_3g019120 62.827 191 68 1 3 190 2 192 5.61e-85 259
MsG0880043648.01.T01 MTR_3g019090 73.239 142 38 0 51 192 1 142 1.16e-79 234
MsG0880043648.01.T01 MTR_3g019120 66.447 152 51 0 39 190 1 152 6.60e-73 227
MsG0880043648.01.T01 MTR_3g019150 76.000 100 24 0 93 192 35 134 6.35e-55 171
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043648.01.T01 AT3G27890 74.866 187 47 0 5 191 10 196 3.30e-101 290

Find 37 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAAAGTGGCAGCTCTTTC+TGG 0.181158 8:-28424299 MsG0880043648.01.T01:CDS
CCATCATCATTGAACTTTGC+AGG 0.314182 8:+28422787 None:intergenic
CATCTAGACCACCAAATGTT+TGG 0.329711 8:-28422951 MsG0880043648.01.T01:CDS
CATTGAAGAATGCAATTGAT+TGG 0.367565 8:-28422975 MsG0880043648.01.T01:CDS
ATCTAGACCACCAAATGTTT+GGG 0.385196 8:-28422950 MsG0880043648.01.T01:CDS
CAATCAATTGCATTCTTCAA+TGG 0.420729 8:+28422976 None:intergenic
GCATTTACTCTGAGACTTCA+AGG 0.432779 8:-28422706 MsG0880043648.01.T01:CDS
TGGCGAAAAGCTTCAACAAC+AGG 0.452851 8:+28424032 None:intergenic
ATTGAAGAATGCAATTGATT+GGG 0.453647 8:-28422974 MsG0880043648.01.T01:CDS
ATAAGCACTGGAGGAGACTT+TGG 0.462138 8:-28422907 MsG0880043648.01.T01:CDS
TTTCCAGCCCAAACATTTGG+TGG 0.464756 8:+28422943 None:intergenic
AAGACGAACCAGCACGGATA+AGG 0.470569 8:+28424246 None:intergenic
GCACTGGAGGAGACTTTGGT+GGG 0.497747 8:-28422903 MsG0880043648.01.T01:CDS
CATTCAGGCCTTATCCGTGC+TGG 0.509003 8:-28424254 MsG0880043648.01.T01:intron
GCAGCAATTGAATTGAGTAA+AGG 0.518179 8:-28424143 MsG0880043648.01.T01:intron
AGACCACCAAATGTTTGGGC+TGG 0.522737 8:-28422946 MsG0880043648.01.T01:CDS
CCTGCAAAGTTCAATGATGA+TGG 0.528301 8:-28422787 MsG0880043648.01.T01:CDS
TTGAGTAAAGGAGCAATTGA+AGG 0.530953 8:-28424131 MsG0880043648.01.T01:CDS
GCAGCTGCAATCATAAGCAC+TGG 0.535634 8:-28422919 MsG0880043648.01.T01:CDS
CAGCACGGATAAGGCCTGAA+TGG 0.546637 8:+28424255 None:intergenic
GAGTATAACTACTCTGTCAC+AGG 0.571097 8:-28423969 MsG0880043648.01.T01:intron
CACTATCATCTACGCCAAGT+TGG 0.576435 8:-28422871 MsG0880043648.01.T01:CDS
TCAAGATCAGTGTTGTACAT+TGG 0.583062 8:+28424074 None:intergenic
AGCACTGGAGGAGACTTTGG+TGG 0.586110 8:-28422904 MsG0880043648.01.T01:CDS
GATCGATATAAATTCCAACT+TGG 0.591111 8:+28422857 None:intergenic
CGAAAGAAGACGAACCAGCA+CGG 0.596751 8:+28424240 None:intergenic
CGAAAAGCTTCAACAACAGG+TGG 0.602230 8:+28424035 None:intergenic
TGGCGATTTGATTGATGAAG+AGG 0.612134 8:-28422767 MsG0880043648.01.T01:CDS
AACACTGATCTTGAAAACAA+AGG 0.627471 8:-28424065 MsG0880043648.01.T01:CDS
AATGCTCTTAGAAAGAACTC+AGG 0.630349 8:+28422817 None:intergenic
ACTGGAGGAGACTTTGGTGG+GGG 0.632673 8:-28422901 MsG0880043648.01.T01:CDS
TCATCATTGAACTTTGCAGG+TGG 0.635212 8:+28422790 None:intergenic
GTGACAGAGTAGTTATACTC+AGG 0.639364 8:+28423972 None:intergenic
CACTGGAGGAGACTTTGGTG+GGG 0.648236 8:-28422902 MsG0880043648.01.T01:CDS
GCTGCAATCATAAGCACTGG+AGG 0.650272 8:-28422916 MsG0880043648.01.T01:CDS
TATCAGCTTGAAGAATCTTG+TGG 0.652286 8:+28424012 None:intergenic
GGCAGCAGCAGTGATCAAAG+TGG 0.706403 8:-28424312 MsG0880043648.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATTGTGAGTTAGAAA+TGG + Chr8:28423926-28423945 None:intergenic 20.0%
!! ATATATATAGTCACATATGT+AGG + Chr8:28423205-28423224 None:intergenic 20.0%
!! ATTATTGTTTCATTCATCAA+GGG + Chr8:28423263-28423282 None:intergenic 20.0%
!! GACTATTAATTTATACATGT+AGG - Chr8:28423293-28423312 MsG0880043648.01.T01:intron 20.0%
!! TGACTATATATATAATACTG+CGG - Chr8:28423212-28423231 MsG0880043648.01.T01:intron 20.0%
! AACCCAAAAAACTACAATTA+TGG + Chr8:28423576-28423595 None:intergenic 25.0%
! ATTGAAGAATGCAATTGATT+GGG - Chr8:28424036-28424055 MsG0880043648.01.T01:CDS 25.0%
! CATTATTGTTTCATTCATCA+AGG + Chr8:28423264-28423283 None:intergenic 25.0%
!!! TCGTTGACATTTTTAGAATT+AGG - Chr8:28423367-28423386 MsG0880043648.01.T01:intron 25.0%
AACACTGATCTTGAAAACAA+AGG - Chr8:28422945-28422964 MsG0880043648.01.T01:CDS 30.0%
AAGAGGAATTTAGTGAATTC+GGG + Chr8:28423469-28423488 None:intergenic 30.0%
ACACAAAACAAAGGTGAAAA+TGG + Chr8:28424008-28424027 None:intergenic 30.0%
ACTATATCTCCGAATTTAGA+AGG + Chr8:28423861-28423880 None:intergenic 30.0%
AGAAAAACTTCATACCATTC+AGG - Chr8:28422741-28422760 MsG0880043648.01.T01:CDS 30.0%
ATGGGATATTTGATTGCAAT+TGG - Chr8:28423529-28423548 MsG0880043648.01.T01:intron 30.0%
ATTGCAATCAAATATCCCAT+TGG + Chr8:28423529-28423548 None:intergenic 30.0%
CAAGAGGAATTTAGTGAATT+CGG + Chr8:28423470-28423489 None:intergenic 30.0%
CAATCAATTGCATTCTTCAA+TGG + Chr8:28424037-28424056 None:intergenic 30.0%
CATTGAAGAATGCAATTGAT+TGG - Chr8:28424035-28424054 MsG0880043648.01.T01:CDS 30.0%
CTATATCTCCGAATTTAGAA+GGG + Chr8:28423860-28423879 None:intergenic 30.0%
GATCGATATAAATTCCAACT+TGG + Chr8:28424156-28424175 None:intergenic 30.0%
GTTCTAATCAAATCACCATT+TGG + Chr8:28423409-28423428 None:intergenic 30.0%
TGACAGTCTCAACATTTAAA+GGG - Chr8:28423606-28423625 MsG0880043648.01.T01:intron 30.0%
TTGACAGTCTCAACATTTAA+AGG - Chr8:28423605-28423624 MsG0880043648.01.T01:intron 30.0%
! ACAAGTTCTTTTATCTTTGC+AGG - Chr8:28424282-28424301 MsG0880043648.01.T01:CDS 30.0%
! ATGGTATGAAGTTTTTCTGA+AGG + Chr8:28422739-28422758 None:intergenic 30.0%
! CGATTTTTGTTCACACAATT+CGG - Chr8:28423170-28423189 MsG0880043648.01.T01:intron 30.0%
! GTTGTAGTATTGAATTTGCA+TGG - Chr8:28422832-28422851 MsG0880043648.01.T01:CDS 30.0%
!! TTGTAGCTTTGAAATGATCA+CGG + Chr8:28423701-28423720 None:intergenic 30.0%
!!! AGGCCATAATTGTAGTTTTT+TGG - Chr8:28423570-28423589 MsG0880043648.01.T01:intron 30.0%
!!! GGCCATAATTGTAGTTTTTT+GGG - Chr8:28423571-28423590 MsG0880043648.01.T01:intron 30.0%
!!! TAGTTTTTTGGGTTGAATCT+CGG - Chr8:28423582-28423601 MsG0880043648.01.T01:intron 30.0%
AATGCTCTTAGAAAGAACTC+AGG + Chr8:28424196-28424215 None:intergenic 35.0%
AGAGGAATTTAGTGAATTCG+GGG + Chr8:28423468-28423487 None:intergenic 35.0%
ATCTAGACCACCAAATGTTT+GGG - Chr8:28424060-28424079 MsG0880043648.01.T01:CDS 35.0%
CACTAAGTCTGTGTTTGATA+AGG + Chr8:28423901-28423920 None:intergenic 35.0%
CGAATTCACTAAATTCCTCT+TGG - Chr8:28423468-28423487 MsG0880043648.01.T01:intron 35.0%
GCAGCAATTGAATTGAGTAA+AGG - Chr8:28422867-28422886 MsG0880043648.01.T01:CDS 35.0%
GCTACAAATTGTCAAAGTGT+AGG - Chr8:28423714-28423733 MsG0880043648.01.T01:intron 35.0%
GTCAAAGTGTAGGAACTTAA+CGG - Chr8:28423724-28423743 MsG0880043648.01.T01:intron 35.0%
TATCAGCTTGAAGAATCTTG+TGG + Chr8:28423001-28423020 None:intergenic 35.0%
TCAAGATCAGTGTTGTACAT+TGG + Chr8:28422939-28422958 None:intergenic 35.0%
TGGAGCTAAACACAAAACAA+AGG + Chr8:28424017-28424036 None:intergenic 35.0%
TTCACTAAATTCCTCTTGGT+AGG - Chr8:28423472-28423491 MsG0880043648.01.T01:intron 35.0%
TTCATTCATCAAGGGTTCAA+GGG + Chr8:28423255-28423274 None:intergenic 35.0%
TTGAGTAAAGGAGCAATTGA+AGG - Chr8:28422879-28422898 MsG0880043648.01.T01:CDS 35.0%
TTTCATTCATCAAGGGTTCA+AGG + Chr8:28423256-28423275 None:intergenic 35.0%
AAACAAGCGCTAAACCTATC+AGG + Chr8:28423639-28423658 None:intergenic 40.0%
AAAGTGTAGGAACTTAACGG+TGG - Chr8:28423727-28423746 MsG0880043648.01.T01:intron 40.0%
AACTGCAGCCCTTCTAAATT+CGG - Chr8:28423849-28423868 MsG0880043648.01.T01:intron 40.0%
ATTGCAAAAACGGCTGCATA+TGG - Chr8:28423758-28423777 MsG0880043648.01.T01:intron 40.0%
CATCTAGACCACCAAATGTT+TGG - Chr8:28424059-28424078 MsG0880043648.01.T01:CDS 40.0%
CCATCATCATTGAACTTTGC+AGG + Chr8:28424226-28424245 None:intergenic 40.0%
CCTGCAAAGTTCAATGATGA+TGG - Chr8:28424223-28424242 MsG0880043648.01.T01:intron 40.0%
GAGTATAACTACTCTGTCAC+AGG - Chr8:28423041-28423060 MsG0880043648.01.T01:intron 40.0%
GCAATTGGATGACTCTAGTT+TGG - Chr8:28423544-28423563 MsG0880043648.01.T01:intron 40.0%
GGACAATCCCATTGCAAAAA+CGG - Chr8:28423748-28423767 MsG0880043648.01.T01:intron 40.0%
GTGACAGAGTAGTTATACTC+AGG + Chr8:28423041-28423060 None:intergenic 40.0%
TCAACTGCAACTCATCCAAA+TGG - Chr8:28423391-28423410 MsG0880043648.01.T01:intron 40.0%
TCATCATTGAACTTTGCAGG+TGG + Chr8:28424223-28424242 None:intergenic 40.0%
TGGCGATTTGATTGATGAAG+AGG - Chr8:28424243-28424262 MsG0880043648.01.T01:intron 40.0%
!! GCATTTACTCTGAGACTTCA+AGG - Chr8:28424304-28424323 MsG0880043648.01.T01:CDS 40.0%
!!! ATATGCAGCCGTTTTTGCAA+TGG + Chr8:28423759-28423778 None:intergenic 40.0%
!!! TATGCAGCCGTTTTTGCAAT+GGG + Chr8:28423758-28423777 None:intergenic 40.0%
AAGCGCTAAACCTATCAGGT+AGG + Chr8:28423635-28423654 None:intergenic 45.0%
AGCGCTAAACCTATCAGGTA+GGG + Chr8:28423634-28423653 None:intergenic 45.0%
ATCAAAGTGGCAGCTCTTTC+TGG - Chr8:28422711-28422730 MsG0880043648.01.T01:CDS 45.0%
CACTATCATCTACGCCAAGT+TGG - Chr8:28424139-28424158 MsG0880043648.01.T01:CDS 45.0%
CGAAAAGCTTCAACAACAGG+TGG + Chr8:28422978-28422997 None:intergenic 45.0%
GACTTTAACCTCGCATGTGA+AGG - Chr8:28423433-28423452 MsG0880043648.01.T01:intron 45.0%
GATTTGAACCTTCACATGCG+AGG + Chr8:28423444-28423463 None:intergenic 45.0%
TGGCGAAAAGCTTCAACAAC+AGG + Chr8:28422981-28423000 None:intergenic 45.0%
TTTCCAGCCCAAACATTTGG+TGG + Chr8:28424070-28424089 None:intergenic 45.0%
! GCTTTTCCAGCCCAAACATT+TGG + Chr8:28424073-28424092 None:intergenic 45.0%
! GGATGACTCTAGTTTGGTCT+AGG - Chr8:28423550-28423569 MsG0880043648.01.T01:intron 45.0%
!! AAATATCCCATTGGTGCCGA+GGG + Chr8:28423520-28423539 None:intergenic 45.0%
!! ATAAGCACTGGAGGAGACTT+TGG - Chr8:28424103-28424122 MsG0880043648.01.T01:CDS 45.0%
!! GAAATGATCACGGTGTCGTT+GGG + Chr8:28423691-28423710 None:intergenic 45.0%
!! TAAAGGGTACCCTACCTGAT+AGG - Chr8:28423622-28423641 MsG0880043648.01.T01:intron 45.0%
!! TGAAATGATCACGGTGTCGT+TGG + Chr8:28423692-28423711 None:intergenic 45.0%
AAACGGCTGCATATGGCTCA+CGG - Chr8:28423765-28423784 MsG0880043648.01.T01:intron 50.0%
AAGACGAACCAGCACGGATA+AGG + Chr8:28422767-28422786 None:intergenic 50.0%
AGACCACCAAATGTTTGGGC+TGG - Chr8:28424064-28424083 MsG0880043648.01.T01:CDS 50.0%
AGGGATGTATCAACATGGGG+GGG + Chr8:28423501-28423520 None:intergenic 50.0%
ATACATCCCTCGGCACCAAT+GGG - Chr8:28423511-28423530 MsG0880043648.01.T01:intron 50.0%
CCCCATGTTGATACATCCCT+CGG - Chr8:28423501-28423520 MsG0880043648.01.T01:intron 50.0%
CCGAGGGATGTATCAACATG+GGG + Chr8:28423504-28423523 None:intergenic 50.0%
CGAAAGAAGACGAACCAGCA+CGG + Chr8:28422773-28422792 None:intergenic 50.0%
CGAGGGATGTATCAACATGG+GGG + Chr8:28423503-28423522 None:intergenic 50.0%
GAGGGATGTATCAACATGGG+GGG + Chr8:28423502-28423521 None:intergenic 50.0%
GCAGCTGCAATCATAAGCAC+TGG - Chr8:28424091-28424110 MsG0880043648.01.T01:CDS 50.0%
GCCGAGGGATGTATCAACAT+GGG + Chr8:28423505-28423524 None:intergenic 50.0%
TGCCGAGGGATGTATCAACA+TGG + Chr8:28423506-28423525 None:intergenic 50.0%
!! CAAATATCCCATTGGTGCCG+AGG + Chr8:28423521-28423540 None:intergenic 50.0%
!! GCTGCAATCATAAGCACTGG+AGG - Chr8:28424094-28424113 MsG0880043648.01.T01:CDS 50.0%
ACTGGAGGAGACTTTGGTGG+GGG - Chr8:28424109-28424128 MsG0880043648.01.T01:CDS 55.0%
CACTGGAGGAGACTTTGGTG+GGG - Chr8:28424108-28424127 MsG0880043648.01.T01:CDS 55.0%
CAGCACGGATAAGGCCTGAA+TGG + Chr8:28422758-28422777 None:intergenic 55.0%
GATACATCCCTCGGCACCAA+TGG - Chr8:28423510-28423529 MsG0880043648.01.T01:intron 55.0%
GCACTGGAGGAGACTTTGGT+GGG - Chr8:28424107-28424126 MsG0880043648.01.T01:CDS 55.0%
GGCAGCAGCAGTGATCAAAG+TGG - Chr8:28422698-28422717 MsG0880043648.01.T01:CDS 55.0%
! AGCACTGGAGGAGACTTTGG+TGG - Chr8:28424106-28424125 MsG0880043648.01.T01:CDS 55.0%
! CATTCAGGCCTTATCCGTGC+TGG - Chr8:28422756-28422775 MsG0880043648.01.T01:CDS 55.0%
TGGGGGGGTTGCCTACCAAG+AGG + Chr8:28423486-28423505 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 28422696 28424336 28422696 ID=MsG0880043648.01;Name=MsG0880043648.01
Chr8 mRNA 28422696 28424336 28422696 ID=MsG0880043648.01.T01;Parent=MsG0880043648.01;Name=MsG0880043648.01.T01;_AED=0.47;_eAED=0.48;_QI=0|0|0|1|1|1|3|0|192
Chr8 exon 28424255 28424336 28424255 ID=MsG0880043648.01.T01:exon:4888;Parent=MsG0880043648.01.T01
Chr8 exon 28423970 28424161 28423970 ID=MsG0880043648.01.T01:exon:4887;Parent=MsG0880043648.01.T01
Chr8 exon 28422696 28423000 28422696 ID=MsG0880043648.01.T01:exon:4886;Parent=MsG0880043648.01.T01
Chr8 CDS 28424255 28424336 28424255 ID=MsG0880043648.01.T01:cds;Parent=MsG0880043648.01.T01
Chr8 CDS 28423970 28424161 28423970 ID=MsG0880043648.01.T01:cds;Parent=MsG0880043648.01.T01
Chr8 CDS 28422696 28423000 28422696 ID=MsG0880043648.01.T01:cds;Parent=MsG0880043648.01.T01
Gene Sequence

>MsG0880043648.01.T01

ATGGCAGCAGCAGTGATCAAAGTGGCAGCTCTTTCTGGTTCCTTCAGAAAAACTTCATACCATTCAGGCCTTATCCGTGCTGCAATTGAATTGAGTAAAGGAGCAATTGAAGGCATTGAGATTGAGTTCATTGATATTTCAACTCTACCAATGTACAACACTGATCTTGAAAACAAAGGAACTTATCCACCTGTTGTTGAAGCTTTTCGCCACAAGATTCTTCAAGCTGATAGTGTTCTCTTTGCTTCTCCTGAGTATAACTACTCTGTCACAGCTCCATTGAAGAATGCAATTGATTGGGCATCTAGACCACCAAATGTTTGGGCTGGAAAAGCAGCTGCAATCATAAGCACTGGAGGAGACTTTGGTGGGGGAAGATCACACTATCATCTACGCCAAGTTGGAATTTATATCGATCTTCATTTCATCAATAAACCTGAGTTCTTTCTAAGAGCATTTGAGCCACCTGCAAAGTTCAATGATGATGGCGATTTGATTGATGAAGAGGCTAAGAATAAGTTGAAACAAGTTCTTTTATCTTTGCAGGCATTTACTCTGAGACTTCAAGGCAAAAACTGA

Protein sequence

>MsG0880043648.01.T01

MAAAVIKVAALSGSFRKTSYHSGLIRAAIELSKGAIEGIEIEFIDISTLPMYNTDLENKGTYPPVVEAFRHKILQADSVLFASPEYNYSVTAPLKNAIDWASRPPNVWAGKAAAIISTGGDFGGGRSHYHLRQVGIYIDLHFINKPEFFLRAFEPPAKFNDDGDLIDEEAKNKLKQVLLSLQAFTLRLQGKN*