AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0119040

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0119040 MtrunA17_Chr4g0003101 60.920 87 28 2 1 87 184 264 5.42e-28 106
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0119040 AT1G15290.1 57.143 49 21 0 1 49 153 201 3.83e-12 61.6
Msa0119040 AT1G15290.2 57.143 49 21 0 1 49 153 201 5.04e-12 61.2

Find 65 sgRNAs with CRISPR-Local

Find 284 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAGTTGTCCCTTCTGGATT+TGG 0.232227 1_3:-42481901 Msa0119040:three_prime_UTR
AAGTTGTCCCTTCTGGATTT+GGG 0.234508 1_3:-42481900 Msa0119040:three_prime_UTR
AGGAGATTACTTTCAGATTC+AGG 0.239023 1_3:-42482820 Msa0119040:intron
GGAGATGAAGTTGTCCCTTC+TGG 0.258232 1_3:-42481907 Msa0119040:three_prime_UTR
TAACAGTTTATCTAGCGATT+AGG 0.277835 1_3:+42483246 None:intergenic
CACAAATTGTGATTCAATAT+CGG 0.293147 1_3:-42483091 Msa0119040:five_prime_UTR
AAGAAACCTTCATTAAATTC+AGG 0.300861 1_3:+42481939 None:intergenic
AACAGTTTATCTAGCGATTA+GGG 0.310080 1_3:+42483247 None:intergenic
ACAAAACACTAGTTATATTA+AGG 0.313616 1_3:+42481068 None:intergenic
CAAGACTAAGAGTAGAAGAT+AGG 0.335113 1_3:-42481129 Msa0119040:three_prime_UTR
ACAACTACAGCTACAATTAC+AGG 0.363670 1_3:-42481837 Msa0119040:intron
TTTGAAGAAATGTGAATTGA+AGG 0.370761 1_3:-42482862 Msa0119040:CDS
CTGTCATTGAAGGGAAGGTT+TGG 0.377281 1_3:-42482492 Msa0119040:intron
ATAGTATATCTTGCCACTTC+CGG 0.397513 1_3:+42482442 None:intergenic
TAGACAACTTGGACTGCATC+TGG 0.405693 1_3:-42482201 Msa0119040:CDS
CTGCATCTGGAAGATCTCTA+TGG 0.414301 1_3:-42482188 Msa0119040:CDS
GTAGTTGTATGTATATCATA+TGG 0.418651 1_3:+42481852 None:intergenic
TTGTGAGGAATTTGTTTACT+TGG 0.445810 1_3:-42483292 Msa0119040:five_prime_UTR
AATTTATTATAAAATATAAG+AGG 0.455639 1_3:+42480929 None:intergenic
GAGGCGGTGAAGCATGCTGA+TGG 0.457396 1_3:-42482470 Msa0119040:CDS
GTCCCTTCTGGATTTGGGGT+TGG 0.458123 1_3:-42481895 Msa0119040:three_prime_UTR
AGAAACCTTCATTAAATTCA+GGG 0.460737 1_3:+42481940 None:intergenic
TTCACTTAGCATTGCTCAAG+AGG 0.463962 1_3:+42482615 None:intergenic
GCTGATGGGGCTGCCGGAAG+TGG 0.468765 1_3:-42482455 Msa0119040:CDS
AGGCGGTGAAGCATGCTGAT+GGG 0.489921 1_3:-42482469 Msa0119040:CDS
GACAAGGATTGAGAAACAAT+TGG 0.492818 1_3:-42481186 Msa0119040:three_prime_UTR
AGGAGCCCTGAATTTAATGA+AGG 0.493408 1_3:-42481945 Msa0119040:three_prime_UTR
TTGAAGGAAGAAGATGATAG+AGG 0.499044 1_3:-42482846 Msa0119040:CDS
TTCATCCGACGCCGAAGTTG+TGG 0.500052 1_3:-42483047 Msa0119040:CDS
CAAAAGACGGGGCAATTACA+AGG 0.508315 1_3:-42481155 Msa0119040:three_prime_UTR
GGCGGTGAAGCATGCTGATG+GGG 0.508593 1_3:-42482468 Msa0119040:CDS
AAGACTAAGAGTAGAAGATA+GGG 0.508621 1_3:-42481128 Msa0119040:three_prime_UTR
AATTTGATATCTAGACAACT+TGG 0.510674 1_3:-42482212 Msa0119040:intron
GAAGAAGATGATAGAGGCAA+AGG 0.512391 1_3:-42482840 Msa0119040:CDS
AAGCATGCTGATGGGGCTGC+CGG 0.516159 1_3:-42482461 Msa0119040:CDS
TTGGCAAAAGAGCAAAAGAC+GGG 0.528765 1_3:-42481167 Msa0119040:three_prime_UTR
TCATCCGACGCCGAAGTTGT+GGG 0.531444 1_3:-42483046 Msa0119040:CDS
TCATTGAAGGGAAGGTTTGG+AGG 0.531755 1_3:-42482489 Msa0119040:intron
ATGAAGGTTTCTTCACCTGT+GGG 0.538137 1_3:-42481929 Msa0119040:three_prime_UTR
AATGAAGGTTTCTTCACCTG+TGG 0.539316 1_3:-42481930 Msa0119040:three_prime_UTR
TGATTCAATATCGGAGAATG+TGG 0.542320 1_3:-42483082 Msa0119040:five_prime_UTR
AGATTGGTAAAAGTTGAAGA+AGG 0.563942 1_3:-42481213 Msa0119040:three_prime_UTR
TGATGAAATGATTGTGAGGA+AGG 0.570504 1_3:-42483339 None:intergenic
AGAGACTCACGCAAAATCAG+AGG 0.576920 1_3:+42482988 None:intergenic
ACAAGTCACCATCAATATGA+AGG 0.583661 1_3:+42481001 None:intergenic
ATCGGAGAATGTGGGAATGG+TGG 0.584217 1_3:-42483073 Msa0119040:exon
AGTTGTCCCTTCTGGATTTG+GGG 0.591313 1_3:-42481899 Msa0119040:three_prime_UTR
AAATCCCACAACTTCGGCGT+CGG 0.597204 1_3:+42483042 None:intergenic
AGTGGCAAGATATACTATGA+AGG 0.597910 1_3:-42482437 Msa0119040:CDS
AATATCGGAGAATGTGGGAA+TGG 0.601867 1_3:-42483076 Msa0119040:exon
GAGACTCACGCAAAATCAGA+GGG 0.608124 1_3:+42482989 None:intergenic
TTGAAGGGAAGGTTTGGAGG+CGG 0.610256 1_3:-42482486 Msa0119040:intron
ACACCAACCCCAAATCCAGA+AGG 0.616176 1_3:+42481892 None:intergenic
ATCTGGAAGATCTCTATGGA+AGG 0.626020 1_3:-42482184 Msa0119040:intron
ATTGTGTTAAAAGAACTTTG+AGG 0.627012 1_3:-42481965 Msa0119040:exon
ATTGGCAAAAGAGCAAAAGA+CGG 0.635114 1_3:-42481168 Msa0119040:three_prime_UTR
GGACAACTTCATCTCCCCAC+AGG 0.640268 1_3:+42481914 None:intergenic
CACCAACCCCAAATCCAGAA+GGG 0.652893 1_3:+42481893 None:intergenic
TGAAGGTTTCTTCACCTGTG+GGG 0.665032 1_3:-42481928 Msa0119040:three_prime_UTR
GATTCAATATCGGAGAATGT+GGG 0.665724 1_3:-42483081 Msa0119040:five_prime_UTR
AGTTGAAGAAGGCAACGACA+AGG 0.669781 1_3:-42481202 Msa0119040:three_prime_UTR
TGTTTGATGAAATGATTGTG+AGG 0.699167 1_3:-42483307 Msa0119040:five_prime_UTR
TGTTTGATGAAATGATTGTG+AGG 0.699167 1_3:-42483343 None:intergenic
TGGCAAAAGAGCAAAAGACG+GGG 0.714282 1_3:-42481166 Msa0119040:three_prime_UTR
AGACTCACGCAAAATCAGAG+GGG 0.782830 1_3:+42482990 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATTTCATTTATTACAT+AGG - chr1_3:42481461-42481480 Msa0119040:intron 10.0%
!! TTTATTTCATTTATTACATA+GGG - chr1_3:42481462-42481481 Msa0119040:intron 10.0%
!!! TATTTGTTTTATATGAAATT+TGG - chr1_3:42481974-42481993 Msa0119040:intron 10.0%
!!! TTTTATTTCATTTATTACAT+AGG - chr1_3:42481461-42481480 Msa0119040:intron 10.0%
!! TTTATTTCATTTATTACATA+GGG - chr1_3:42481462-42481481 Msa0119040:intron 10.0%
!!! TATTTGTTTTATATGAAATT+TGG - chr1_3:42481974-42481993 Msa0119040:intron 10.0%
!! AAATTTAACAAACAAAAACA+GGG + chr1_3:42481698-42481717 None:intergenic 15.0%
!! TTATGAAAATAAATAGAACA+AGG + chr1_3:42482189-42482208 None:intergenic 15.0%
!!! ATTTTCATAATTTCAATTTG+AGG - chr1_3:42482199-42482218 Msa0119040:CDS 15.0%
!! AAATTTAACAAACAAAAACA+GGG + chr1_3:42481698-42481717 None:intergenic 15.0%
!! TTATGAAAATAAATAGAACA+AGG + chr1_3:42482189-42482208 None:intergenic 15.0%
!!! ATTTTCATAATTTCAATTTG+AGG - chr1_3:42482199-42482218 Msa0119040:CDS 15.0%
!!! AATTTTTATGCGTTTTTGTT+AGG - chr1_3:42481312-42481331 Msa0119040:intron 20.0%
!!! ATTTTTATGCGTTTTTGTTA+GGG - chr1_3:42481313-42481332 Msa0119040:intron 20.0%
!! GAAATTTAACAAACAAAAAC+AGG + chr1_3:42481699-42481718 None:intergenic 20.0%
!! ATAAAGATATGTCTACAATT+TGG - chr1_3:42482113-42482132 Msa0119040:intron 20.0%
!! TGCTATTAACATCAAATATA+GGG + chr1_3:42482257-42482276 None:intergenic 20.0%
!! TATATATATGATTTATCCCA+AGG - chr1_3:42482555-42482574 Msa0119040:intron 20.0%
!! ATATATATGATTTATCCCAA+GGG - chr1_3:42482556-42482575 Msa0119040:intron 20.0%
!! ATAAAGTTTATGCAAGTTAA+AGG + chr1_3:42482823-42482842 None:intergenic 20.0%
!! TAAAAAGAAAAACAGAATTC+TGG + chr1_3:42482883-42482902 None:intergenic 20.0%
!!! GAATTCTGTTTTTCTTTTTA+AGG - chr1_3:42482883-42482902 Msa0119040:intron 20.0%
!!! TTTTTGTTTGATCAATCATA+TGG - chr1_3:42483003-42483022 Msa0119040:CDS 20.0%
!! ACAAAACACTAGTTATATTA+AGG + chr1_3:42483180-42483199 None:intergenic 20.0%
!!! ATGTATTTTATGACTAATGT+CGG - chr1_3:42483285-42483304 Msa0119040:five_prime_UTR 20.0%
!!! AATTTTTATGCGTTTTTGTT+AGG - chr1_3:42481312-42481331 Msa0119040:intron 20.0%
!!! ATTTTTATGCGTTTTTGTTA+GGG - chr1_3:42481313-42481332 Msa0119040:intron 20.0%
!! GAAATTTAACAAACAAAAAC+AGG + chr1_3:42481699-42481718 None:intergenic 20.0%
!! ATAAAGATATGTCTACAATT+TGG - chr1_3:42482113-42482132 Msa0119040:intron 20.0%
!! TGCTATTAACATCAAATATA+GGG + chr1_3:42482257-42482276 None:intergenic 20.0%
!! TATATATATGATTTATCCCA+AGG - chr1_3:42482555-42482574 Msa0119040:intron 20.0%
!! ATATATATGATTTATCCCAA+GGG - chr1_3:42482556-42482575 Msa0119040:intron 20.0%
!! ATAAAGTTTATGCAAGTTAA+AGG + chr1_3:42482823-42482842 None:intergenic 20.0%
!! TAAAAAGAAAAACAGAATTC+TGG + chr1_3:42482883-42482902 None:intergenic 20.0%
!!! GAATTCTGTTTTTCTTTTTA+AGG - chr1_3:42482883-42482902 Msa0119040:intron 20.0%
!!! TTTTTGTTTGATCAATCATA+TGG - chr1_3:42483003-42483022 Msa0119040:CDS 20.0%
!! ACAAAACACTAGTTATATTA+AGG + chr1_3:42483180-42483199 None:intergenic 20.0%
!!! ATGTATTTTATGACTAATGT+CGG - chr1_3:42483285-42483304 Msa0119040:five_prime_UTR 20.0%
! CACAAATTGTGATTCAATAT+CGG - chr1_3:42481154-42481173 Msa0119040:three_prime_UTR 25.0%
! TTTGAAGAAATGTGAATTGA+AGG - chr1_3:42481383-42481402 Msa0119040:intron 25.0%
! GCTACAAAATAGACTAATAA+GGG + chr1_3:42481594-42481613 None:intergenic 25.0%
! TGCTACAAAATAGACTAATA+AGG + chr1_3:42481595-42481614 None:intergenic 25.0%
!!! TATTAGTCTATTTTGTAGCA+TGG - chr1_3:42481595-42481614 Msa0119040:intron 25.0%
!!! TGCTAAGTGAATTTTAGTTT+TGG - chr1_3:42481642-42481661 Msa0119040:intron 25.0%
!!! GGCTTTGAATATAATGATAT+AGG + chr1_3:42481883-42481902 None:intergenic 25.0%
! TCATTATATTCAAAGCCAAT+AGG - chr1_3:42481886-42481905 Msa0119040:three_prime_UTR 25.0%
! AAACAAAAAATAGCACCTAT+TGG + chr1_3:42481904-42481923 None:intergenic 25.0%
!!! AGGTGCTATTTTTTGTTTTT+AGG - chr1_3:42481906-42481925 Msa0119040:three_prime_UTR 25.0%
! TCTAATCAATATGTCATTGA+AGG + chr1_3:42481941-42481960 None:intergenic 25.0%
! AATTTGATATCTAGACAACT+TGG - chr1_3:42482033-42482052 Msa0119040:intron 25.0%
!! GTCTACAATTTGGAAAATTT+TGG - chr1_3:42482123-42482142 Msa0119040:intron 25.0%
! CTGCTATTAACATCAAATAT+AGG + chr1_3:42482258-42482277 None:intergenic 25.0%
! ATTGTGTTAAAAGAACTTTG+AGG - chr1_3:42482280-42482299 Msa0119040:intron 25.0%
! AGAAACCTTCATTAAATTCA+GGG + chr1_3:42482308-42482327 None:intergenic 25.0%
! AAGAAACCTTCATTAAATTC+AGG + chr1_3:42482309-42482328 None:intergenic 25.0%
!!! CATATGGTTTTTTGATTGTT+GGG + chr1_3:42482380-42482399 None:intergenic 25.0%
!!! TCATATGGTTTTTTGATTGT+TGG + chr1_3:42482381-42482400 None:intergenic 25.0%
! GTAGTTGTATGTATATCATA+TGG + chr1_3:42482396-42482415 None:intergenic 25.0%
! TATTTGTAGCTATTCAAAGT+GGG + chr1_3:42482774-42482793 None:intergenic 25.0%
! ATATTTGTAGCTATTCAAAG+TGG + chr1_3:42482775-42482794 None:intergenic 25.0%
!!! AATCATATGGTTTTGTAGAT+TGG - chr1_3:42483016-42483035 Msa0119040:CDS 25.0%
!! ATTTTACACCTTCATATTGA+TGG - chr1_3:42483236-42483255 Msa0119040:five_prime_UTR 25.0%
!! TGTATTTTATGACTAATGTC+GGG - chr1_3:42483286-42483305 Msa0119040:five_prime_UTR 25.0%
! CACAAATTGTGATTCAATAT+CGG - chr1_3:42481154-42481173 Msa0119040:three_prime_UTR 25.0%
! TTTGAAGAAATGTGAATTGA+AGG - chr1_3:42481383-42481402 Msa0119040:intron 25.0%
! GCTACAAAATAGACTAATAA+GGG + chr1_3:42481594-42481613 None:intergenic 25.0%
! TGCTACAAAATAGACTAATA+AGG + chr1_3:42481595-42481614 None:intergenic 25.0%
!!! TATTAGTCTATTTTGTAGCA+TGG - chr1_3:42481595-42481614 Msa0119040:intron 25.0%
!!! TGCTAAGTGAATTTTAGTTT+TGG - chr1_3:42481642-42481661 Msa0119040:intron 25.0%
!!! GGCTTTGAATATAATGATAT+AGG + chr1_3:42481883-42481902 None:intergenic 25.0%
! TCATTATATTCAAAGCCAAT+AGG - chr1_3:42481886-42481905 Msa0119040:three_prime_UTR 25.0%
! AAACAAAAAATAGCACCTAT+TGG + chr1_3:42481904-42481923 None:intergenic 25.0%
!!! AGGTGCTATTTTTTGTTTTT+AGG - chr1_3:42481906-42481925 Msa0119040:three_prime_UTR 25.0%
! TCTAATCAATATGTCATTGA+AGG + chr1_3:42481941-42481960 None:intergenic 25.0%
! AATTTGATATCTAGACAACT+TGG - chr1_3:42482033-42482052 Msa0119040:intron 25.0%
!! GTCTACAATTTGGAAAATTT+TGG - chr1_3:42482123-42482142 Msa0119040:intron 25.0%
! CTGCTATTAACATCAAATAT+AGG + chr1_3:42482258-42482277 None:intergenic 25.0%
! ATTGTGTTAAAAGAACTTTG+AGG - chr1_3:42482280-42482299 Msa0119040:intron 25.0%
! AGAAACCTTCATTAAATTCA+GGG + chr1_3:42482308-42482327 None:intergenic 25.0%
! AAGAAACCTTCATTAAATTC+AGG + chr1_3:42482309-42482328 None:intergenic 25.0%
!!! CATATGGTTTTTTGATTGTT+GGG + chr1_3:42482380-42482399 None:intergenic 25.0%
!!! TCATATGGTTTTTTGATTGT+TGG + chr1_3:42482381-42482400 None:intergenic 25.0%
! GTAGTTGTATGTATATCATA+TGG + chr1_3:42482396-42482415 None:intergenic 25.0%
! TATTTGTAGCTATTCAAAGT+GGG + chr1_3:42482774-42482793 None:intergenic 25.0%
! ATATTTGTAGCTATTCAAAG+TGG + chr1_3:42482775-42482794 None:intergenic 25.0%
!!! AATCATATGGTTTTGTAGAT+TGG - chr1_3:42483016-42483035 Msa0119040:CDS 25.0%
!! ATTTTACACCTTCATATTGA+TGG - chr1_3:42483236-42483255 Msa0119040:five_prime_UTR 25.0%
!! TGTATTTTATGACTAATGTC+GGG - chr1_3:42483286-42483305 Msa0119040:five_prime_UTR 25.0%
TGTTTGATGAAATGATTGTG+AGG - chr1_3:42480938-42480957 Msa0119040:three_prime_UTR 30.0%
! TTGTGAGGAATTTGTTTACT+TGG - chr1_3:42480953-42480972 Msa0119040:three_prime_UTR 30.0%
AACAGTTTATCTAGCGATTA+GGG + chr1_3:42481001-42481020 None:intergenic 30.0%
TAACAGTTTATCTAGCGATT+AGG + chr1_3:42481002-42481021 None:intergenic 30.0%
! GCAATGAATCAACTTTTCAA+AGG + chr1_3:42481047-42481066 None:intergenic 30.0%
TCATAAAAATCCCACAACTT+CGG + chr1_3:42481212-42481231 None:intergenic 30.0%
AACATTTGACATGGTGATAA+GGG - chr1_3:42481339-42481358 Msa0119040:intron 30.0%
GCAACAATAACAATATTGCA+GGG + chr1_3:42481516-42481535 None:intergenic 30.0%
TGCAACAATAACAATATTGC+AGG + chr1_3:42481517-42481536 None:intergenic 30.0%
TTAACAAACAAAAACAGGGA+AGG + chr1_3:42481694-42481713 None:intergenic 30.0%
ATACAAAACTCTGTCATTGA+AGG - chr1_3:42481743-42481762 Msa0119040:intron 30.0%
TACAAAACTCTGTCATTGAA+GGG - chr1_3:42481744-42481763 Msa0119040:intron 30.0%
ATTCATTGTTATAGTCCCTT+GGG + chr1_3:42482574-42482593 None:intergenic 30.0%
AATTAGAGATGTAGAGAAGT+AGG + chr1_3:42482620-42482639 None:intergenic 30.0%
TACTTCTCTACATCTCTAAT+TGG - chr1_3:42482619-42482638 Msa0119040:intron 30.0%
ACTTCTCTACATCTCTAATT+GGG - chr1_3:42482620-42482639 Msa0119040:CDS 30.0%
TGTTTGCATTTCATTGAGTT+AGG - chr1_3:42482670-42482689 Msa0119040:intron 30.0%
GAGTAAAAGAAAGACTCTAA+AGG + chr1_3:42482962-42482981 None:intergenic 30.0%
AGATTGGTAAAAGTTGAAGA+AGG - chr1_3:42483032-42483051 Msa0119040:CDS 30.0%
AAGACTAAGAGTAGAAGATA+GGG - chr1_3:42483117-42483136 Msa0119040:five_prime_UTR 30.0%
TGTTTGATGAAATGATTGTG+AGG - chr1_3:42480938-42480957 Msa0119040:three_prime_UTR 30.0%
! TTGTGAGGAATTTGTTTACT+TGG - chr1_3:42480953-42480972 Msa0119040:three_prime_UTR 30.0%
AACAGTTTATCTAGCGATTA+GGG + chr1_3:42481001-42481020 None:intergenic 30.0%
TAACAGTTTATCTAGCGATT+AGG + chr1_3:42481002-42481021 None:intergenic 30.0%
! GCAATGAATCAACTTTTCAA+AGG + chr1_3:42481047-42481066 None:intergenic 30.0%
TCATAAAAATCCCACAACTT+CGG + chr1_3:42481212-42481231 None:intergenic 30.0%
AACATTTGACATGGTGATAA+GGG - chr1_3:42481339-42481358 Msa0119040:intron 30.0%
GCAACAATAACAATATTGCA+GGG + chr1_3:42481516-42481535 None:intergenic 30.0%
TGCAACAATAACAATATTGC+AGG + chr1_3:42481517-42481536 None:intergenic 30.0%
TTAACAAACAAAAACAGGGA+AGG + chr1_3:42481694-42481713 None:intergenic 30.0%
ATACAAAACTCTGTCATTGA+AGG - chr1_3:42481743-42481762 Msa0119040:intron 30.0%
TACAAAACTCTGTCATTGAA+GGG - chr1_3:42481744-42481763 Msa0119040:intron 30.0%
ATTCATTGTTATAGTCCCTT+GGG + chr1_3:42482574-42482593 None:intergenic 30.0%
AATTAGAGATGTAGAGAAGT+AGG + chr1_3:42482620-42482639 None:intergenic 30.0%
TACTTCTCTACATCTCTAAT+TGG - chr1_3:42482619-42482638 Msa0119040:intron 30.0%
ACTTCTCTACATCTCTAATT+GGG - chr1_3:42482620-42482639 Msa0119040:CDS 30.0%
TGTTTGCATTTCATTGAGTT+AGG - chr1_3:42482670-42482689 Msa0119040:intron 30.0%
GAGTAAAAGAAAGACTCTAA+AGG + chr1_3:42482962-42482981 None:intergenic 30.0%
AGATTGGTAAAAGTTGAAGA+AGG - chr1_3:42483032-42483051 Msa0119040:CDS 30.0%
AAGACTAAGAGTAGAAGATA+GGG - chr1_3:42483117-42483136 Msa0119040:five_prime_UTR 30.0%
TGATTCAATATCGGAGAATG+TGG - chr1_3:42481163-42481182 Msa0119040:three_prime_UTR 35.0%
GATTCAATATCGGAGAATGT+GGG - chr1_3:42481164-42481183 Msa0119040:three_prime_UTR 35.0%
TTAGGGTTGAACATTTGACA+TGG - chr1_3:42481330-42481349 Msa0119040:intron 35.0%
GAACATTTGACATGGTGATA+AGG - chr1_3:42481338-42481357 Msa0119040:intron 35.0%
TTGAAGGAAGAAGATGATAG+AGG - chr1_3:42481399-42481418 Msa0119040:intron 35.0%
AGGAGATTACTTTCAGATTC+AGG - chr1_3:42481425-42481444 Msa0119040:intron 35.0%
! ATGTGTCTGATTTAAAGCTG+AGG - chr1_3:42481536-42481555 Msa0119040:intron 35.0%
ACAAAAACAGGGAAGGAATA+AGG + chr1_3:42481687-42481706 None:intergenic 35.0%
! GTTTGTTAAATTTCCGATCG+TGG - chr1_3:42481705-42481724 Msa0119040:intron 35.0%
!! GAGTTTTGTATGACCAGAAT+TGG + chr1_3:42481734-42481753 None:intergenic 35.0%
! ATAGTATATCTTGCCACTTC+CGG + chr1_3:42481806-42481825 None:intergenic 35.0%
AGTGGCAAGATATACTATGA+AGG - chr1_3:42481808-42481827 Msa0119040:intron 35.0%
! CTGCAATGCTATATGTTTTC+AGG - chr1_3:42481843-42481862 Msa0119040:three_prime_UTR 35.0%
ACAACTACAGCTACAATTAC+AGG - chr1_3:42482408-42482427 Msa0119040:CDS 35.0%
CATTCATTGTTATAGTCCCT+TGG + chr1_3:42482575-42482594 None:intergenic 35.0%
AGGGACTATAACAATGAATG+AGG - chr1_3:42482575-42482594 Msa0119040:intron 35.0%
ATGCAAGTTAAAGGAACTCA+CGG + chr1_3:42482814-42482833 None:intergenic 35.0%
!! CTAAAGGCACTTCATTGTTT+AGG + chr1_3:42482946-42482965 None:intergenic 35.0%
! CTTTCTTTTACTCTTCTCAG+AGG - chr1_3:42482969-42482988 Msa0119040:intron 35.0%
GACAAGGATTGAGAAACAAT+TGG - chr1_3:42483059-42483078 Msa0119040:CDS 35.0%
ATTGGCAAAAGAGCAAAAGA+CGG - chr1_3:42483077-42483096 Msa0119040:exon 35.0%
CAAGACTAAGAGTAGAAGAT+AGG - chr1_3:42483116-42483135 Msa0119040:five_prime_UTR 35.0%
ACAAGTCACCATCAATATGA+AGG + chr1_3:42483247-42483266 None:intergenic 35.0%
TGATTCAATATCGGAGAATG+TGG - chr1_3:42481163-42481182 Msa0119040:three_prime_UTR 35.0%
GATTCAATATCGGAGAATGT+GGG - chr1_3:42481164-42481183 Msa0119040:three_prime_UTR 35.0%
TTAGGGTTGAACATTTGACA+TGG - chr1_3:42481330-42481349 Msa0119040:intron 35.0%
GAACATTTGACATGGTGATA+AGG - chr1_3:42481338-42481357 Msa0119040:intron 35.0%
TTGAAGGAAGAAGATGATAG+AGG - chr1_3:42481399-42481418 Msa0119040:intron 35.0%
AGGAGATTACTTTCAGATTC+AGG - chr1_3:42481425-42481444 Msa0119040:intron 35.0%
! ATGTGTCTGATTTAAAGCTG+AGG - chr1_3:42481536-42481555 Msa0119040:intron 35.0%
ACAAAAACAGGGAAGGAATA+AGG + chr1_3:42481687-42481706 None:intergenic 35.0%
! GTTTGTTAAATTTCCGATCG+TGG - chr1_3:42481705-42481724 Msa0119040:intron 35.0%
!! GAGTTTTGTATGACCAGAAT+TGG + chr1_3:42481734-42481753 None:intergenic 35.0%
! ATAGTATATCTTGCCACTTC+CGG + chr1_3:42481806-42481825 None:intergenic 35.0%
AGTGGCAAGATATACTATGA+AGG - chr1_3:42481808-42481827 Msa0119040:intron 35.0%
! CTGCAATGCTATATGTTTTC+AGG - chr1_3:42481843-42481862 Msa0119040:three_prime_UTR 35.0%
ACAACTACAGCTACAATTAC+AGG - chr1_3:42482408-42482427 Msa0119040:CDS 35.0%
CATTCATTGTTATAGTCCCT+TGG + chr1_3:42482575-42482594 None:intergenic 35.0%
AGGGACTATAACAATGAATG+AGG - chr1_3:42482575-42482594 Msa0119040:intron 35.0%
ATGCAAGTTAAAGGAACTCA+CGG + chr1_3:42482814-42482833 None:intergenic 35.0%
!! CTAAAGGCACTTCATTGTTT+AGG + chr1_3:42482946-42482965 None:intergenic 35.0%
! CTTTCTTTTACTCTTCTCAG+AGG - chr1_3:42482969-42482988 Msa0119040:intron 35.0%
GACAAGGATTGAGAAACAAT+TGG - chr1_3:42483059-42483078 Msa0119040:CDS 35.0%
ATTGGCAAAAGAGCAAAAGA+CGG - chr1_3:42483077-42483096 Msa0119040:exon 35.0%
CAAGACTAAGAGTAGAAGAT+AGG - chr1_3:42483116-42483135 Msa0119040:five_prime_UTR 35.0%
ACAAGTCACCATCAATATGA+AGG + chr1_3:42483247-42483266 None:intergenic 35.0%
AATATCGGAGAATGTGGGAA+TGG - chr1_3:42481169-42481188 Msa0119040:three_prime_UTR 40.0%
GAAGAAGATGATAGAGGCAA+AGG - chr1_3:42481405-42481424 Msa0119040:intron 40.0%
GCAACAACATTTACACTCTC+AGG + chr1_3:42481562-42481581 None:intergenic 40.0%
TTCACTTAGCATTGCTCAAG+AGG + chr1_3:42481633-42481652 None:intergenic 40.0%
AAACTCTGTCATTGAAGGGA+AGG - chr1_3:42481748-42481767 Msa0119040:intron 40.0%
ATCTGGAAGATCTCTATGGA+AGG - chr1_3:42482061-42482080 Msa0119040:intron 40.0%
AGGAGCCCTGAATTTAATGA+AGG - chr1_3:42482300-42482319 Msa0119040:intron 40.0%
AATGAAGGTTTCTTCACCTG+TGG - chr1_3:42482315-42482334 Msa0119040:intron 40.0%
ATGAAGGTTTCTTCACCTGT+GGG - chr1_3:42482316-42482335 Msa0119040:intron 40.0%
AAGTTGTCCCTTCTGGATTT+GGG - chr1_3:42482345-42482364 Msa0119040:intron 40.0%
CATCTCTAATTGGGTGTGAA+TGG - chr1_3:42482629-42482648 Msa0119040:CDS 40.0%
AAGTTAAAGGAACTCACGGT+GGG + chr1_3:42482810-42482829 None:intergenic 40.0%
AATTCTGGTACTAGAGAGTC+AGG + chr1_3:42482868-42482887 None:intergenic 40.0%
TTGGCAAAAGAGCAAAAGAC+GGG - chr1_3:42483078-42483097 Msa0119040:exon 40.0%
AATATCGGAGAATGTGGGAA+TGG - chr1_3:42481169-42481188 Msa0119040:three_prime_UTR 40.0%
GAAGAAGATGATAGAGGCAA+AGG - chr1_3:42481405-42481424 Msa0119040:intron 40.0%
GCAACAACATTTACACTCTC+AGG + chr1_3:42481562-42481581 None:intergenic 40.0%
TTCACTTAGCATTGCTCAAG+AGG + chr1_3:42481633-42481652 None:intergenic 40.0%
AAACTCTGTCATTGAAGGGA+AGG - chr1_3:42481748-42481767 Msa0119040:intron 40.0%
ATCTGGAAGATCTCTATGGA+AGG - chr1_3:42482061-42482080 Msa0119040:intron 40.0%
AGGAGCCCTGAATTTAATGA+AGG - chr1_3:42482300-42482319 Msa0119040:intron 40.0%
AATGAAGGTTTCTTCACCTG+TGG - chr1_3:42482315-42482334 Msa0119040:intron 40.0%
ATGAAGGTTTCTTCACCTGT+GGG - chr1_3:42482316-42482335 Msa0119040:intron 40.0%
AAGTTGTCCCTTCTGGATTT+GGG - chr1_3:42482345-42482364 Msa0119040:intron 40.0%
CATCTCTAATTGGGTGTGAA+TGG - chr1_3:42482629-42482648 Msa0119040:CDS 40.0%
AAGTTAAAGGAACTCACGGT+GGG + chr1_3:42482810-42482829 None:intergenic 40.0%
AATTCTGGTACTAGAGAGTC+AGG + chr1_3:42482868-42482887 None:intergenic 40.0%
TTGGCAAAAGAGCAAAAGAC+GGG - chr1_3:42483078-42483097 Msa0119040:exon 40.0%
AGACTCACGCAAAATCAGAG+GGG + chr1_3:42481258-42481277 None:intergenic 45.0%
GAGACTCACGCAAAATCAGA+GGG + chr1_3:42481259-42481278 None:intergenic 45.0%
AGAGACTCACGCAAAATCAG+AGG + chr1_3:42481260-42481279 None:intergenic 45.0%
CCAGAATTGGATTCCACGAT+CGG + chr1_3:42481721-42481740 None:intergenic 45.0%
CTGTCATTGAAGGGAAGGTT+TGG - chr1_3:42481753-42481772 Msa0119040:intron 45.0%
TCATTGAAGGGAAGGTTTGG+AGG - chr1_3:42481756-42481775 Msa0119040:intron 45.0%
TAGACAACTTGGACTGCATC+TGG - chr1_3:42482044-42482063 Msa0119040:intron 45.0%
CTGCATCTGGAAGATCTCTA+TGG - chr1_3:42482057-42482076 Msa0119040:intron 45.0%
TGAAGGTTTCTTCACCTGTG+GGG - chr1_3:42482317-42482336 Msa0119040:intron 45.0%
GAAGTTGTCCCTTCTGGATT+TGG - chr1_3:42482344-42482363 Msa0119040:intron 45.0%
AGTTGTCCCTTCTGGATTTG+GGG - chr1_3:42482346-42482365 Msa0119040:intron 45.0%
TCTCTATCTGTCATTGCACC+CGG - chr1_3:42482464-42482483 Msa0119040:CDS 45.0%
!!! ACTCATCATGCTGCTTTTCC+GGG + chr1_3:42482485-42482504 None:intergenic 45.0%
!!! GACTCATCATGCTGCTTTTC+CGG + chr1_3:42482486-42482505 None:intergenic 45.0%
CTAATTGGGTGTGAATGGAG+AGG - chr1_3:42482634-42482653 Msa0119040:CDS 45.0%
GTGAATGGAGAGGTAGATCT+CGG - chr1_3:42482644-42482663 Msa0119040:intron 45.0%
AATGTGTGATGCTGCTGTCT+TGG - chr1_3:42482742-42482761 Msa0119040:intron 45.0%
AGTTAAAGGAACTCACGGTG+GGG + chr1_3:42482809-42482828 None:intergenic 45.0%
CAAGTTAAAGGAACTCACGG+TGG + chr1_3:42482811-42482830 None:intergenic 45.0%
AGTTGAAGAAGGCAACGACA+AGG - chr1_3:42483043-42483062 Msa0119040:CDS 45.0%
TGGCAAAAGAGCAAAAGACG+GGG - chr1_3:42483079-42483098 Msa0119040:exon 45.0%
CAAAAGACGGGGCAATTACA+AGG - chr1_3:42483090-42483109 Msa0119040:five_prime_UTR 45.0%
AGACTCACGCAAAATCAGAG+GGG + chr1_3:42481258-42481277 None:intergenic 45.0%
GAGACTCACGCAAAATCAGA+GGG + chr1_3:42481259-42481278 None:intergenic 45.0%
AGAGACTCACGCAAAATCAG+AGG + chr1_3:42481260-42481279 None:intergenic 45.0%
CCAGAATTGGATTCCACGAT+CGG + chr1_3:42481721-42481740 None:intergenic 45.0%
CTGTCATTGAAGGGAAGGTT+TGG - chr1_3:42481753-42481772 Msa0119040:intron 45.0%
TCATTGAAGGGAAGGTTTGG+AGG - chr1_3:42481756-42481775 Msa0119040:intron 45.0%
TAGACAACTTGGACTGCATC+TGG - chr1_3:42482044-42482063 Msa0119040:intron 45.0%
CTGCATCTGGAAGATCTCTA+TGG - chr1_3:42482057-42482076 Msa0119040:intron 45.0%
TGAAGGTTTCTTCACCTGTG+GGG - chr1_3:42482317-42482336 Msa0119040:intron 45.0%
GAAGTTGTCCCTTCTGGATT+TGG - chr1_3:42482344-42482363 Msa0119040:intron 45.0%
AGTTGTCCCTTCTGGATTTG+GGG - chr1_3:42482346-42482365 Msa0119040:intron 45.0%
TCTCTATCTGTCATTGCACC+CGG - chr1_3:42482464-42482483 Msa0119040:CDS 45.0%
!!! ACTCATCATGCTGCTTTTCC+GGG + chr1_3:42482485-42482504 None:intergenic 45.0%
!!! GACTCATCATGCTGCTTTTC+CGG + chr1_3:42482486-42482505 None:intergenic 45.0%
CTAATTGGGTGTGAATGGAG+AGG - chr1_3:42482634-42482653 Msa0119040:CDS 45.0%
GTGAATGGAGAGGTAGATCT+CGG - chr1_3:42482644-42482663 Msa0119040:intron 45.0%
AATGTGTGATGCTGCTGTCT+TGG - chr1_3:42482742-42482761 Msa0119040:intron 45.0%
AGTTAAAGGAACTCACGGTG+GGG + chr1_3:42482809-42482828 None:intergenic 45.0%
CAAGTTAAAGGAACTCACGG+TGG + chr1_3:42482811-42482830 None:intergenic 45.0%
AGTTGAAGAAGGCAACGACA+AGG - chr1_3:42483043-42483062 Msa0119040:CDS 45.0%
TGGCAAAAGAGCAAAAGACG+GGG - chr1_3:42483079-42483098 Msa0119040:exon 45.0%
CAAAAGACGGGGCAATTACA+AGG - chr1_3:42483090-42483109 Msa0119040:five_prime_UTR 45.0%
!! AATTTATTATAAAATATAAG+AGG + chr1_3:42483319-42483338 None:intergenic 5.0%
!! AATTTATTATAAAATATAAG+AGG + chr1_3:42483319-42483338 None:intergenic 5.0%
ATCGGAGAATGTGGGAATGG+TGG - chr1_3:42481172-42481191 Msa0119040:three_prime_UTR 50.0%
AAATCCCACAACTTCGGCGT+CGG + chr1_3:42481206-42481225 None:intergenic 50.0%
CCGATCGTGGAATCCAATTC+TGG - chr1_3:42481718-42481737 Msa0119040:intron 50.0%
TTGAAGGGAAGGTTTGGAGG+CGG - chr1_3:42481759-42481778 Msa0119040:intron 50.0%
GGAGATGAAGTTGTCCCTTC+TGG - chr1_3:42482338-42482357 Msa0119040:intron 50.0%
CACCAACCCCAAATCCAGAA+GGG + chr1_3:42482355-42482374 None:intergenic 50.0%
ACACCAACCCCAAATCCAGA+AGG + chr1_3:42482356-42482375 None:intergenic 50.0%
AATGGAGAGGTAGATCTCGG+CGG - chr1_3:42482647-42482666 Msa0119040:intron 50.0%
TGGTACTAGAGAGTCAGGCA+CGG + chr1_3:42482863-42482882 None:intergenic 50.0%
ATCGGAGAATGTGGGAATGG+TGG - chr1_3:42481172-42481191 Msa0119040:three_prime_UTR 50.0%
AAATCCCACAACTTCGGCGT+CGG + chr1_3:42481206-42481225 None:intergenic 50.0%
CCGATCGTGGAATCCAATTC+TGG - chr1_3:42481718-42481737 Msa0119040:intron 50.0%
TTGAAGGGAAGGTTTGGAGG+CGG - chr1_3:42481759-42481778 Msa0119040:intron 50.0%
GGAGATGAAGTTGTCCCTTC+TGG - chr1_3:42482338-42482357 Msa0119040:intron 50.0%
CACCAACCCCAAATCCAGAA+GGG + chr1_3:42482355-42482374 None:intergenic 50.0%
ACACCAACCCCAAATCCAGA+AGG + chr1_3:42482356-42482375 None:intergenic 50.0%
AATGGAGAGGTAGATCTCGG+CGG - chr1_3:42482647-42482666 Msa0119040:intron 50.0%
TGGTACTAGAGAGTCAGGCA+CGG + chr1_3:42482863-42482882 None:intergenic 50.0%
TTCATCCGACGCCGAAGTTG+TGG - chr1_3:42481198-42481217 Msa0119040:three_prime_UTR 55.0%
TCATCCGACGCCGAAGTTGT+GGG - chr1_3:42481199-42481218 Msa0119040:three_prime_UTR 55.0%
AGGCGGTGAAGCATGCTGAT+GGG - chr1_3:42481776-42481795 Msa0119040:intron 55.0%
GGACAACTTCATCTCCCCAC+AGG + chr1_3:42482334-42482353 None:intergenic 55.0%
! GTCCCTTCTGGATTTGGGGT+TGG - chr1_3:42482350-42482369 Msa0119040:intron 55.0%
TTCATCCGACGCCGAAGTTG+TGG - chr1_3:42481198-42481217 Msa0119040:three_prime_UTR 55.0%
TCATCCGACGCCGAAGTTGT+GGG - chr1_3:42481199-42481218 Msa0119040:three_prime_UTR 55.0%
AGGCGGTGAAGCATGCTGAT+GGG - chr1_3:42481776-42481795 Msa0119040:intron 55.0%
GGACAACTTCATCTCCCCAC+AGG + chr1_3:42482334-42482353 None:intergenic 55.0%
! GTCCCTTCTGGATTTGGGGT+TGG - chr1_3:42482350-42482369 Msa0119040:intron 55.0%
GAGGCGGTGAAGCATGCTGA+TGG - chr1_3:42481775-42481794 Msa0119040:intron 60.0%
GGCGGTGAAGCATGCTGATG+GGG - chr1_3:42481777-42481796 Msa0119040:intron 60.0%
AAGCATGCTGATGGGGCTGC+CGG - chr1_3:42481784-42481803 Msa0119040:intron 60.0%
GAGGCGGTGAAGCATGCTGA+TGG - chr1_3:42481775-42481794 Msa0119040:intron 60.0%
GGCGGTGAAGCATGCTGATG+GGG - chr1_3:42481777-42481796 Msa0119040:intron 60.0%
AAGCATGCTGATGGGGCTGC+CGG - chr1_3:42481784-42481803 Msa0119040:intron 60.0%
GCTGATGGGGCTGCCGGAAG+TGG - chr1_3:42481790-42481809 Msa0119040:intron 70.0%
GCTGATGGGGCTGCCGGAAG+TGG - chr1_3:42481790-42481809 Msa0119040:intron 70.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 42480914 42483353 42480914 ID=Msa0119040;Name=Msa0119040
chr1_3 mRNA 42480914 42483353 42480914 ID=Msa0119040-mRNA-1;Parent=Msa0119040;Name=Msa0119040-mRNA-1;_AED=0.21;_eAED=0.21;_QI=274|0.5|0.71|1|0.66|0.71|7|460|109
chr1_3 exon 42480914 42481233 42480914 ID=Msa0119040-mRNA-1:exon:12826;Parent=Msa0119040-mRNA-1
chr1_3 exon 42481838 42481989 42481838 ID=Msa0119040-mRNA-1:exon:12825;Parent=Msa0119040-mRNA-1
chr1_3 exon 42482185 42482220 42482185 ID=Msa0119040-mRNA-1:exon:12824;Parent=Msa0119040-mRNA-1
chr1_3 exon 42482403 42482502 42482403 ID=Msa0119040-mRNA-1:exon:12823;Parent=Msa0119040-mRNA-1
chr1_3 exon 42482620 42482654 42482620 ID=Msa0119040-mRNA-1:exon:12822;Parent=Msa0119040-mRNA-1
chr1_3 exon 42482821 42482885 42482821 ID=Msa0119040-mRNA-1:exon:12821;Parent=Msa0119040-mRNA-1
chr1_3 exon 42482998 42483353 42482998 ID=Msa0119040-mRNA-1:exon:12820;Parent=Msa0119040-mRNA-1
chr1_3 five_prime_UTR 42483080 42483353 42483080 ID=Msa0119040-mRNA-1:five_prime_utr;Parent=Msa0119040-mRNA-1
chr1_3 CDS 42482998 42483079 42482998 ID=Msa0119040-mRNA-1:cds;Parent=Msa0119040-mRNA-1
chr1_3 CDS 42482821 42482885 42482821 ID=Msa0119040-mRNA-1:cds;Parent=Msa0119040-mRNA-1
chr1_3 CDS 42482620 42482654 42482620 ID=Msa0119040-mRNA-1:cds;Parent=Msa0119040-mRNA-1
chr1_3 CDS 42482403 42482502 42482403 ID=Msa0119040-mRNA-1:cds;Parent=Msa0119040-mRNA-1
chr1_3 CDS 42482185 42482220 42482185 ID=Msa0119040-mRNA-1:cds;Parent=Msa0119040-mRNA-1
chr1_3 CDS 42481978 42481989 42481978 ID=Msa0119040-mRNA-1:cds;Parent=Msa0119040-mRNA-1
chr1_3 three_prime_UTR 42481838 42481977 42481838 ID=Msa0119040-mRNA-1:three_prime_utr;Parent=Msa0119040-mRNA-1
chr1_3 three_prime_UTR 42480914 42481233 42480914 ID=Msa0119040-mRNA-1:three_prime_utr;Parent=Msa0119040-mRNA-1
Gene Sequence

>Msa0119040

ATGGTGGCGATTCATCCGACGCCGAAGTTGTGGGATTTTTATGAGTTCTTTTCGTTTTCGAATCTTACCCCTCTGATTTTGCATTTGAAGAAATGTGAATTGAAGGAAGAAGATGATAGAGGCAAAGGAGATTACTTTCAGATTCAGCATGGTTTTAGTGAGATCCTCTTGAGCAATGCTAAGGAAGGTTTGGAGGCGGTGAAGCATGCTGATGGGGCTGCCGGAAGTGGCAAGATATACTATGAAGGTGAGAGTGTGATCTGCAATGCTATATGTTTTCAGACAACTTGGACTGCATCTGGAAGATCTCTATGGAAGAAATTGTGTTAA
Protein sequence

>Msa0119040

MVAIHPTPKLWDFYEFFSFSNLTPLILHLKKCELKEEDDRGKGDYFQIQHGFSEILLSNAKEGLEAVKHADGAAGSGKIYYEGESVICNAICFQTTWTASGRSLWKKLC*