AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0697040


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0697040 MtrunA17_Chr4g0049651 95.588 68 3 0 1 68 1 68 3.62e-43 145
Msa0697040 MtrunA17_Chr4g0049671 72.059 68 10 1 1 68 1 59 2.58e-26 99.8
Msa0697040 MtrunA17_Chr4g0049701 80.769 52 10 0 17 68 14 65 1.06e-22 87.8
Msa0697040 MtrunA17_Chr4g0049691 50.450 111 11 3 1 67 1 111 5.42e-22 81.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0697040 AT1G74520.1 43.939 66 37 0 2 67 4 69 1.31e-11 56.6
Msa0697040 AT1G74520.2 43.939 66 37 0 2 67 4 69 1.31e-11 56.6
Msa0697040 AT5G62490.1 47.541 61 32 0 7 67 9 69 3.35e-11 55.5

Find 27 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTATTCAGGTCGTAGGATT+TGG 0.253838 4_4:-67022611 Msa0697040:five_prime_UTR
GTTATAGTACGGATTTATTC+AGG 0.269672 4_4:-67022624 Msa0697040:five_prime_UTR
AGCTTGAATTTGGTAGTAAA+TGG 0.281705 4_4:-67022396 Msa0697040:exon
TTCTTCATGGTTTCTCAAAC+TGG 0.286518 4_4:-67022372 Msa0697040:CDS
TTATTCAGGTCGTAGGATTT+GGG 0.299422 4_4:-67022610 Msa0697040:five_prime_UTR
CATACCATGCAAAATGATAA+AGG 0.301623 4_4:+67022339 None:intergenic
TTCCATTCGGAGCTTGAATT+TGG 0.308560 4_4:-67022406 Msa0697040:five_prime_UTR
CTCCGAATGGAAAAGGAAAA+TGG 0.330831 4_4:+67022417 None:intergenic
CCAAGGATTTGATCTCATAT+TGG 0.353877 4_4:-67022020 Msa0697040:CDS
GGTAGTAAATGGCTTCTTCA+TGG 0.362420 4_4:-67022385 Msa0697040:exon
ATATTCAAAGAGGTAAATTA+AGG 0.374191 4_4:+67021987 None:intergenic
AAATAGTAAAGTAGCTTAGT+TGG 0.385187 4_4:+67022471 None:intergenic
CCAATATGAGATCAAATCCT+TGG 0.466673 4_4:+67022020 None:intergenic
GTCAGTTTCAATTGCTTGTA+CGG 0.466858 4_4:+67022056 None:intergenic
ATTCAAGCTCCGAATGGAAA+AGG 0.483180 4_4:+67022410 None:intergenic
AGTAAAGTAGCTTAGTTGGT+AGG 0.504642 4_4:+67022475 None:intergenic
GACACGGAGCAAAATGTCAT+TGG 0.524996 4_4:+67022530 None:intergenic
AAAGAATTTACCGTAGAAGA+AGG 0.542698 4_4:+67021953 None:intergenic
TACGGATTTATTCAGGTCGT+AGG 0.564676 4_4:-67022617 Msa0697040:five_prime_UTR
TACCAAATTCAAGCTCCGAA+TGG 0.564717 4_4:+67022404 None:intergenic
CTTAGTTGGTAGGTTGCAAG+TGG 0.575855 4_4:+67022485 None:intergenic
GTTGGTAGGTTGCAAGTGGG+AGG 0.596186 4_4:+67022489 None:intergenic
TATTCAGGTCGTAGGATTTG+GGG 0.598701 4_4:-67022609 Msa0697040:five_prime_UTR
TCATAAAAGCATATTCAAAG+AGG 0.621411 4_4:+67021977 None:intergenic
TGACTCATATGCAGAAACCA+AGG 0.655333 4_4:-67022037 Msa0697040:CDS
TTAGTTGGTAGGTTGCAAGT+GGG 0.704476 4_4:+67022486 None:intergenic
GAAAATAAGATATACCGACA+CGG 0.728030 4_4:+67022514 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTATTCTATCTACTTTT+GGG + chr4_4:67021971-67021990 None:intergenic 15.0%
!!! TTTTTTATTCTATCTACTTT+TGG + chr4_4:67021972-67021991 None:intergenic 15.0%
!! GAATAAAAAAAGTTATAGTA+CGG - chr4_4:67021982-67022001 Msa0697040:CDS 15.0%
!!! TTTTTGTTTTTTTGTGATTA+TGG - chr4_4:67022519-67022538 Msa0697040:five_prime_UTR 15.0%
!!! TTTTATTCTATCTACTTTTG+GGG + chr4_4:67021970-67021989 None:intergenic 20.0%
!! GAAAAAGATGAAGAAAATAA+AGG + chr4_4:67022326-67022345 None:intergenic 20.0%
!! TGTCAAAACAATAATGTAAA+AGG + chr4_4:67022480-67022499 None:intergenic 20.0%
!!! TTTTTTGTGATTATGGTTTT+TGG - chr4_4:67022526-67022545 Msa0697040:five_prime_UTR 20.0%
!! ATATTCAAAGAGGTAAATTA+AGG + chr4_4:67022633-67022652 None:intergenic 20.0%
! AAATAGTAAAGTAGCTTAGT+TGG + chr4_4:67022149-67022168 None:intergenic 25.0%
! AAGATGAAGAAAATAAAGGA+GGG + chr4_4:67022322-67022341 None:intergenic 25.0%
! AAAGATGAAGAAAATAAAGG+AGG + chr4_4:67022323-67022342 None:intergenic 25.0%
!! TTATTTCCTTCTCTTTTTCT+AGG - chr4_4:67022384-67022403 Msa0697040:exon 25.0%
!! TCATAAAAGCATATTCAAAG+AGG + chr4_4:67022643-67022662 None:intergenic 25.0%
GTTATAGTACGGATTTATTC+AGG - chr4_4:67021993-67022012 Msa0697040:CDS 30.0%
GAAAATAAGATATACCGACA+CGG + chr4_4:67022106-67022125 None:intergenic 30.0%
! TTCCATTTTCCTTTTCCATT+CGG - chr4_4:67022198-67022217 Msa0697040:intron 30.0%
! AGCTTGAATTTGGTAGTAAA+TGG - chr4_4:67022221-67022240 Msa0697040:intron 30.0%
! AATGCCTTTATCATTTTGCA+TGG - chr4_4:67022274-67022293 Msa0697040:intron 30.0%
CATACCATGCAAAATGATAA+AGG + chr4_4:67022281-67022300 None:intergenic 30.0%
TTTATTCAGGTCGTAGGATT+TGG - chr4_4:67022006-67022025 Msa0697040:CDS 35.0%
TTATTCAGGTCGTAGGATTT+GGG - chr4_4:67022007-67022026 Msa0697040:CDS 35.0%
AGAAGCAGAAAAAACAAGAG+AGG - chr4_4:67022034-67022053 Msa0697040:CDS 35.0%
GAAGCAGAAAAAACAAGAGA+GGG - chr4_4:67022035-67022054 Msa0697040:CDS 35.0%
AGTAAAGTAGCTTAGTTGGT+AGG + chr4_4:67022145-67022164 None:intergenic 35.0%
TTCTTCATGGTTTCTCAAAC+TGG - chr4_4:67022245-67022264 Msa0697040:intron 35.0%
TCCTATGTAAGTTCTTGATC+TGG - chr4_4:67022427-67022446 Msa0697040:five_prime_UTR 35.0%
!! TGTGATTATGGTTTTTGGAC+AGG - chr4_4:67022531-67022550 Msa0697040:five_prime_UTR 35.0%
GTCAGTTTCAATTGCTTGTA+CGG + chr4_4:67022564-67022583 None:intergenic 35.0%
CCAATATGAGATCAAATCCT+TGG + chr4_4:67022600-67022619 None:intergenic 35.0%
CCAAGGATTTGATCTCATAT+TGG - chr4_4:67022597-67022616 Msa0697040:five_prime_UTR 35.0%
! CTTTTATGAGCCTTCTTCTA+CGG - chr4_4:67022654-67022673 Msa0697040:five_prime_UTR 35.0%
TACGGATTTATTCAGGTCGT+AGG - chr4_4:67022000-67022019 Msa0697040:CDS 40.0%
TATTCAGGTCGTAGGATTTG+GGG - chr4_4:67022008-67022027 Msa0697040:CDS 40.0%
CGTTCGCATTTACTTCAAGT+AGG + chr4_4:67022062-67022081 None:intergenic 40.0%
ACTTGAAGTAAATGCGAACG+AGG - chr4_4:67022062-67022081 Msa0697040:CDS 40.0%
CTTGAAGTAAATGCGAACGA+GGG - chr4_4:67022063-67022082 Msa0697040:CDS 40.0%
! AATGACATTTTGCTCCGTGT+CGG - chr4_4:67022089-67022108 Msa0697040:intron 40.0%
!! TTAGTTGGTAGGTTGCAAGT+GGG + chr4_4:67022134-67022153 None:intergenic 40.0%
CTCCGAATGGAAAAGGAAAA+TGG + chr4_4:67022203-67022222 None:intergenic 40.0%
ATTCAAGCTCCGAATGGAAA+AGG + chr4_4:67022210-67022229 None:intergenic 40.0%
TTCCATTCGGAGCTTGAATT+TGG - chr4_4:67022211-67022230 Msa0697040:CDS 40.0%
TACCAAATTCAAGCTCCGAA+TGG + chr4_4:67022216-67022235 None:intergenic 40.0%
GGTAGTAAATGGCTTCTTCA+TGG - chr4_4:67022232-67022251 Msa0697040:intron 40.0%
AAGAGGCCTAGAAAAAGAGA+AGG + chr4_4:67022393-67022412 None:intergenic 40.0%
GCCAGATCAAGAACTTACAT+AGG + chr4_4:67022431-67022450 None:intergenic 40.0%
AAGTTCTTGATCTGGCTGTT+TGG - chr4_4:67022435-67022454 Msa0697040:five_prime_UTR 40.0%
TGACTCATATGCAGAAACCA+AGG - chr4_4:67022580-67022599 Msa0697040:intron 40.0%
GACACGGAGCAAAATGTCAT+TGG + chr4_4:67022090-67022109 None:intergenic 45.0%
!! CTTAGTTGGTAGGTTGCAAG+TGG + chr4_4:67022135-67022154 None:intergenic 45.0%
! TTCTAGGCCTCTTTTTGCTC+TGG - chr4_4:67022400-67022419 Msa0697040:five_prime_UTR 45.0%
GGATACACCAGAGCAAAAAG+AGG + chr4_4:67022410-67022429 None:intergenic 45.0%
! GTTGGTAGGTTGCAAGTGGG+AGG + chr4_4:67022131-67022150 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr4_4 gene 67021960 67022679 67021960 ID=Msa0697040;Name=Msa0697040
chr4_4 mRNA 67021960 67022679 67021960 ID=Msa0697040-mRNA-1;Parent=Msa0697040;Name=Msa0697040-mRNA-1;_AED=0.48;_eAED=0.48;_QI=252|1|0.75|1|0|0|4|0|69
chr4_4 exon 67021960 67022086 67021960 ID=Msa0697040-mRNA-1:exon:19598;Parent=Msa0697040-mRNA-1
chr4_4 exon 67022207 67022233 67022207 ID=Msa0697040-mRNA-1:exon:19597;Parent=Msa0697040-mRNA-1
chr4_4 exon 67022344 67022555 67022344 ID=Msa0697040-mRNA-1:exon:19596;Parent=Msa0697040-mRNA-1
chr4_4 exon 67022584 67022679 67022584 ID=Msa0697040-mRNA-1:exon:19595;Parent=Msa0697040-mRNA-1
chr4_4 five_prime_UTR 67022584 67022679 67022584 ID=Msa0697040-mRNA-1:five_prime_utr;Parent=Msa0697040-mRNA-1
chr4_4 five_prime_UTR 67022400 67022555 67022400 ID=Msa0697040-mRNA-1:five_prime_utr;Parent=Msa0697040-mRNA-1
chr4_4 CDS 67022344 67022399 67022344 ID=Msa0697040-mRNA-1:cds;Parent=Msa0697040-mRNA-1
chr4_4 CDS 67022207 67022233 67022207 ID=Msa0697040-mRNA-1:cds;Parent=Msa0697040-mRNA-1
chr4_4 CDS 67021960 67022086 67021960 ID=Msa0697040-mRNA-1:cds;Parent=Msa0697040-mRNA-1
Gene Sequence

>Msa0697040

ATGGCTTCTTCATGGTTTCTCAAACTGGCTTTCAAATGCCTTTATCATTTTGCATGGCCTCTTTTTGCTCTGGTGTATCCTATGTGTGCTTCCGTACAAGCAATTGAAACTGACTCATATGCAGAAACCAAGGATTTGATCTCATATTGGATACTTCTTTCCTTAATTTACCTCTTTGAATATGCTTTTATGAGCCTTCTTCTACGGTAA

Protein sequence

>Msa0697040

MASSWFLKLAFKCLYHFAWPLFALVYPMCASVQAIETDSYAETKDLISYWILLSLIYLFEYAFMSLLLR*