AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0553940

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0553940 MtrunA17_Chr4g0039791 93.056 72 5 0 50 121 490 561 2.45e-40 140
Msa0553940 MtrunA17_Chr5g0409771 71.765 85 24 0 37 121 471 555 1.53e-33 122
Msa0553940 MtrunA17_Chr5g0409791 84.615 65 10 0 57 121 504 568 3.22e-31 115
Msa0553940 MtrunA17_Chr1g0197391 58.442 77 28 1 49 121 494 570 6.56e-21 86.7
Msa0553940 MtrunA17_Chr1g0157401 56.522 69 30 0 53 121 492 560 9.92e-21 86.3
Msa0553940 MtrunA17_Chr3g0116251 59.016 61 25 0 53 113 488 548 5.03e-20 84.3
Msa0553940 MtrunA17_Chr3g0116271 59.016 61 25 0 53 113 490 550 5.77e-20 84.0
Msa0553940 MtrunA17_Chr1g0180551 42.268 97 47 1 26 113 517 613 1.54e-17 77.0
Msa0553940 MtrunA17_Chr1g0186071 52.459 61 29 0 53 113 488 548 4.74e-17 75.5
Msa0553940 MtrunA17_Chr4g0072561 41.176 85 50 0 37 121 464 548 1.55e-16 74.3
Msa0553940 MtrunA17_Chr1g0180541 50.769 65 32 0 49 113 476 540 1.41e-15 71.6
Msa0553940 MtrunA17_Chr4g0000461 50.000 68 33 1 53 119 473 540 1.87e-15 71.2
Msa0553940 MtrunA17_Chr2g0334131 45.833 72 38 1 49 119 211 282 4.51e-15 69.3
Msa0553940 MtrunA17_Chr5g0395431 41.379 87 51 0 33 119 499 585 1.79e-14 68.2
Msa0553940 MtrunA17_Chr2g0327341 42.029 69 40 0 53 121 471 539 1.93e-14 68.2
Msa0553940 MtrunA17_Chr4g0028411 44.262 61 34 0 53 113 474 534 1.99e-14 68.2
Msa0553940 MtrunA17_Chr3g0102411 46.377 69 37 0 53 121 473 541 1.11e-13 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0553940 AT4G19010.1 51.020 98 42 2 25 121 470 562 2.24e-22 91.3
Msa0553940 AT5G63380.1 57.377 61 26 0 53 113 490 550 1.50e-19 83.2
Msa0553940 AT1G20510.1 57.377 61 26 0 53 113 476 536 2.44e-19 82.4
Msa0553940 AT1G51680.1 46.067 89 44 1 33 121 477 561 4.58e-18 79.0
Msa0553940 AT3G21240.1 42.697 89 47 1 33 121 470 554 1.87e-17 77.4
Msa0553940 AT1G20500.1 52.174 69 33 0 53 121 482 550 2.24e-17 77.0
Msa0553940 AT1G20480.1 49.275 69 35 0 53 121 497 565 3.22e-17 76.6
Msa0553940 AT5G38120.1 54.098 61 28 0 53 113 482 542 4.01e-17 76.3
Msa0553940 AT1G65060.1 51.515 66 32 0 49 114 492 557 5.53e-16 73.2
Msa0553940 AT4G05160.1 39.326 89 54 0 33 121 456 544 1.23e-15 72.0
Msa0553940 AT3G21230.1 45.070 71 39 0 49 119 496 566 2.21e-13 65.5
Msa0553940 AT1G62940.1 50.000 62 31 0 53 114 472 533 4.93e-12 61.6

Find 212 sgRNAs with CRISPR-Local

Find 318 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCTTCTGCTTTACACAAAA+TGG 0.138296 4_1:+51224174 Msa0553940:five_prime_UTR
TAAATATCACCATGACTTTC+TGG 0.142879 4_1:-51223158 None:intergenic
ACAGGGTAATAAATTGAATT+TGG 0.183276 4_1:-51224232 None:intergenic
CTCTTGGCTGTTCTGTATTT+AGG 0.185255 4_1:+51224259 Msa0553940:five_prime_UTR
ATATGACCAATAATGAATTT+TGG 0.185466 4_1:+51223477 Msa0553940:five_prime_UTR
GCCCTTATTGATTCTTCATC+TGG 0.217618 4_1:+51224106 Msa0553940:five_prime_UTR
TAATCATAATGTTGGGGATT+TGG 0.219662 4_1:-51223700 None:intergenic
TAAAATAGTGAAATAGAAAA+AGG 0.223449 4_1:-51223820 None:intergenic
GGAGTTTGATTAAAAGAGTT+TGG 0.228239 4_1:-51223395 None:intergenic
CGCCATGTCTTGTCTTTCTT+TGG 0.230322 4_1:-51227083 None:intergenic
TGTTGGGGATTTGGAATATT+TGG 0.231225 4_1:-51223691 None:intergenic
TATCAAATACAAGGGATTTC+AGG 0.243292 4_1:+51226384 Msa0553940:five_prime_UTR
GTTGAGTGATCATGTTGTTT+TGG 0.245364 4_1:-51223952 None:intergenic
TTTCATGAATTATAGGATAT+TGG 0.247502 4_1:+51227811 Msa0553940:three_prime_UTR
TAATATCCAAAATTCATTAT+TGG 0.251066 4_1:-51223483 None:intergenic
TTGACATTGACGAGGTTATC+AGG 0.253484 4_1:+51224773 Msa0553940:five_prime_UTR
TCATAGAAATCTTATTTCTA+TGG 0.256021 4_1:+51224594 Msa0553940:five_prime_UTR
CTTCATCTTCGGCACTGATA+TGG 0.264781 4_1:-51226709 None:intergenic
AGACAAAAGAATACAATTTC+AGG 0.266973 4_1:+51228012 Msa0553940:three_prime_UTR
ACGGCTGCATCGACTATTTC+AGG 0.272026 4_1:-51226556 None:intergenic
AGTGCCGAAGATGAAGAAAT+TGG 0.290280 4_1:+51226716 Msa0553940:CDS
TTTCACAACTATTCGTCAAT+AGG 0.295826 4_1:+51225592 Msa0553940:five_prime_UTR
GGGTCAGTTGGAAGATTTCT+AGG 0.298899 4_1:-51224031 None:intergenic
TTAATACCTTATTCTTCTTC+AGG 0.300163 4_1:+51227538 Msa0553940:three_prime_UTR
ATGAAGGCAAAGGGTGTTAA+TGG 0.303488 4_1:+51224859 Msa0553940:five_prime_UTR
AATCTTCTAAAGTGCCTCTT+TGG 0.304882 4_1:+51226650 Msa0553940:CDS
TGGAAATTTCTGTACGCATT+TGG 0.315063 4_1:-51223618 None:intergenic
AATATTTGGTGTGAAGAAAA+AGG 0.315910 4_1:-51223677 None:intergenic
AGAACAGCCAAGAGTATAAC+AGG 0.326675 4_1:-51224250 None:intergenic
CAGGCAGCTGCGGTGAGCTT+TGG 0.328932 4_1:+51225677 Msa0553940:five_prime_UTR
CAAAGCTCACCGCAGCTGCC+TGG 0.332403 4_1:-51225676 None:intergenic
TTGCCAATGTTTCATGTGTA+TGG 0.342668 4_1:+51224688 Msa0553940:five_prime_UTR
AGTCCACCAGTTTCATGTTA+TGG 0.343233 4_1:-51227111 None:intergenic
GGAACGTAATAGCTACGTTT+AGG 0.343445 4_1:-51223728 None:intergenic
AATGTGCAGAATAGAAATTT+TGG 0.344789 4_1:-51227210 None:intergenic
ACCCAGATGAAGAATCAATA+AGG 0.350199 4_1:-51224108 None:intergenic
ACTTGTGCGGTTGTGCCTTA+AGG 0.351894 4_1:-51223230 None:intergenic
AGCAGTGCCTGAGTTGCTTT+CGG 0.356544 4_1:-51227010 None:intergenic
CTTGTGCGGTTGTGCCTTAA+GGG 0.359633 4_1:-51223229 None:intergenic
AAGGGTGAAATTGTCTTTGT+TGG 0.360994 4_1:-51223658 None:intergenic
GGGACTATAATCATAATGTT+GGG 0.361470 4_1:-51223707 None:intergenic
GCTGGTATATTGTATTCATC+TGG 0.361976 4_1:+51224538 Msa0553940:five_prime_UTR
CTCCTAAAATATAACTCTTT+AGG 0.365677 4_1:+51223414 Msa0553940:five_prime_UTR
TTCCCCAATTTCTTCATCTT+CGG 0.365786 4_1:-51226720 None:intergenic
TTTGTTGGGATCCTAACTAT+AGG 0.368496 4_1:-51223643 None:intergenic
TCTTTGCCTGAAGAAGAATA+AGG 0.373133 4_1:-51227544 None:intergenic
TTCTTACCTCTCATAATAGA+AGG 0.374837 4_1:-51225709 None:intergenic
CAGTAATAACAAAATCATTC+AGG 0.375459 4_1:+51227599 Msa0553940:three_prime_UTR
AAATTTCCAACCCTATAGTT+AGG 0.375908 4_1:+51223632 Msa0553940:five_prime_UTR
TTCACAACTATTCGTCAATA+GGG 0.376437 4_1:+51225593 Msa0553940:five_prime_UTR
ACCTGTATAAAATCAACGTT+AGG 0.376743 4_1:-51224967 None:intergenic
TGTTGGTGAAATTCGTAAAC+AGG 0.387052 4_1:+51224315 Msa0553940:five_prime_UTR
TTCACTTGTAATCTTTGATA+AGG 0.390882 4_1:+51227502 Msa0553940:three_prime_UTR
AAAGCTCACCGCAGCTGCCT+GGG 0.391998 4_1:-51225675 None:intergenic
TTGTTGGGATCCTAACTATA+GGG 0.394896 4_1:-51223642 None:intergenic
GAGTTTGATTAAAAGAGTTT+GGG 0.395162 4_1:-51223394 None:intergenic
GAACGTAATAGCTACGTTTA+GGG 0.395890 4_1:-51223727 None:intergenic
TTATTACCCTGTTATACTCT+TGG 0.395992 4_1:+51224243 Msa0553940:five_prime_UTR
ACCATAAGTCTCATGTAGAT+TGG 0.396872 4_1:+51227935 Msa0553940:three_prime_UTR
GTGCCGAAGATGAAGAAATT+GGG 0.397388 4_1:+51226717 Msa0553940:CDS
AGGGTGAAATTGTCTTTGTT+GGG 0.398587 4_1:-51223657 None:intergenic
TACTCTGCCCTGTACACCTT+TGG 0.399980 4_1:+51227303 Msa0553940:three_prime_UTR
TGCTGCGGGATTATTGTCGT+TGG 0.401502 4_1:+51224723 Msa0553940:five_prime_UTR
CCCAATCTACATGAGACTTA+TGG 0.402140 4_1:-51227936 None:intergenic
ACCTTCCGGACCTTCTTGTA+TGG 0.403187 4_1:-51226942 None:intergenic
ATTAAACAAAACCAAGGAAA+AGG 0.409840 4_1:-51223537 None:intergenic
TGTTGTTCCACCGATGTTGT+CGG 0.411176 4_1:+51224828 Msa0553940:five_prime_UTR
AAAGAAACAATGTCAAGAAA+AGG 0.413052 4_1:-51224052 None:intergenic
CCCTTATTGATTCTTCATCT+GGG 0.414327 4_1:+51224107 Msa0553940:five_prime_UTR
TGCTTGAGTTCTAAACTCTA+AGG 0.416696 4_1:+51227029 Msa0553940:CDS
TCAGGATGGATATTTACATT+TGG 0.418131 4_1:+51226342 Msa0553940:five_prime_UTR
GTCAAGAAACTAGAGCTATT+AGG 0.418468 4_1:+51224382 Msa0553940:five_prime_UTR
TCAAAGGGTAGTTATTAAAC+AGG 0.419883 4_1:+51224507 Msa0553940:five_prime_UTR
CTATTCGTCAATAGGGTTGT+TGG 0.424322 4_1:+51225600 Msa0553940:five_prime_UTR
AGAGGCACTTTAGAAGATTA+CGG 0.425783 4_1:-51226646 None:intergenic
GACAACATCGGTGGAACAAC+AGG 0.427365 4_1:-51224826 None:intergenic
TACGCATTTGGCTCTCAAGT+TGG 0.428976 4_1:-51223606 None:intergenic
TGTTTAATAACTACCCTTTG+AGG 0.430394 4_1:-51224505 None:intergenic
GGTTTATCGCTCTTTGCTGC+GGG 0.430597 4_1:+51224709 Msa0553940:five_prime_UTR
ACGCATTTGGCTCTCAAGTT+GGG 0.434343 4_1:-51223605 None:intergenic
TAACAACTTTGAGTAAGAAA+TGG 0.436153 4_1:-51224134 None:intergenic
TTTGTTGAAAGTGATGGATT+CGG 0.436910 4_1:-51227771 None:intergenic
AGGGACTATAATCATAATGT+TGG 0.443278 4_1:-51223708 None:intergenic
CAGAAAATGAGAAGGATTTG+AGG 0.445513 4_1:+51224431 Msa0553940:five_prime_UTR
GCTGCGGGATTATTGTCGTT+GGG 0.447735 4_1:+51224724 Msa0553940:five_prime_UTR
CTCAACACCCTCACCCTCAT+TGG 0.448612 4_1:+51223969 Msa0553940:five_prime_UTR
TGCGGTGAGCTTTGGTTGAG+AGG 0.454034 4_1:+51225685 Msa0553940:five_prime_UTR
GCTTCTGCTTTACACAAAAT+GGG 0.455667 4_1:+51224175 Msa0553940:five_prime_UTR
GGGTTACCATAACATGAAAC+TGG 0.457748 4_1:+51227105 Msa0553940:three_prime_UTR
CCATAAGTCTCATGTAGATT+GGG 0.460455 4_1:+51227936 Msa0553940:three_prime_UTR
ATAACTGACCAAAGGTGTAC+AGG 0.460940 4_1:-51227311 None:intergenic
CCCAGATGAAGAATCAATAA+GGG 0.467167 4_1:-51224107 None:intergenic
AATCAAATTTACCATAAAGA+AGG 0.470429 4_1:-51224480 None:intergenic
GCATTTGTTGTCAAGAAGGC+TGG 0.471765 4_1:+51226752 Msa0553940:CDS
ATTGAAAAGTATGACACAAC+AGG 0.473687 4_1:-51227445 None:intergenic
ATTATTGTCGTTGGGCTCTA+CGG 0.478036 4_1:+51224732 Msa0553940:five_prime_UTR
AGTCGTTGGTGTACAATACT+TGG 0.482534 4_1:+51227702 Msa0553940:three_prime_UTR
TAAATTTGATTTGCCTCAAA+GGG 0.485047 4_1:+51224492 Msa0553940:five_prime_UTR
GTAAATTTGATTTGCCTCAA+AGG 0.487083 4_1:+51224491 Msa0553940:five_prime_UTR
AACAGCTAGAAACACACTCT+TGG 0.487245 4_1:-51224665 None:intergenic
ACATAATCGACCACATGCTT+CGG 0.487322 4_1:-51226788 None:intergenic
TTGTATTCATCTGGGACTAC+AGG 0.489553 4_1:+51224547 Msa0553940:five_prime_UTR
ATTTCACCAACACTACTAAG+AGG 0.489624 4_1:-51224304 None:intergenic
CGGAGACCTTGGTATCTTGT+CGG 0.489928 4_1:-51226973 None:intergenic
AGTGGCATTTGTTGTCAAGA+AGG 0.496940 4_1:+51226748 Msa0553940:CDS
AAGAAACAATGTCAAGAAAA+GGG 0.497596 4_1:-51224051 None:intergenic
GTACAGGGCAGAGTAATTGA+TGG 0.498066 4_1:-51227295 None:intergenic
AACACAGCTTCTAGATCAGC+AGG 0.500308 4_1:-51226523 None:intergenic
CACAAAATGGGTGTTTCACA+AGG 0.502968 4_1:+51224187 Msa0553940:five_prime_UTR
TTGAATCCTCTTAGTAGTGT+TGG 0.505325 4_1:+51224298 Msa0553940:five_prime_UTR
TTAAACAAAACCAAGGAAAA+GGG 0.505625 4_1:-51223536 None:intergenic
TTGAGCATAGGAGTTATTAG+TGG 0.506442 4_1:+51224928 Msa0553940:five_prime_UTR
ATCTTCCCAGTCGGAGACCT+TGG 0.507080 4_1:-51226984 None:intergenic
TGGTTTATCGCTCTTTGCTG+CGG 0.507976 4_1:+51224708 Msa0553940:five_prime_UTR
ATTTCTCTAGTGCATTTACA+AGG 0.509552 4_1:-51227344 None:intergenic
TGTCGGTGAAGAACACCTTC+CGG 0.510625 4_1:-51226956 None:intergenic
TAAGTTGCAGAGTTTGAGAC+AGG 0.511530 4_1:+51224885 Msa0553940:five_prime_UTR
TTGCTCCATACAAGAAGGTC+CGG 0.515213 4_1:+51226937 Msa0553940:CDS
CGGAGGCAAAAGTGGTAGAC+TGG 0.515561 4_1:+51225632 Msa0553940:five_prime_UTR
AAAGATGGTTGGCTACATAC+AGG 0.515947 4_1:+51226301 Msa0553940:five_prime_UTR
GCGGTGAGCTTTGGTTGAGA+GGG 0.517348 4_1:+51225686 Msa0553940:five_prime_UTR
ACATCTGAATTATGAGTCGT+TGG 0.521217 4_1:+51227688 Msa0553940:three_prime_UTR
TTTGTCTTTATCATGTGTGC+GGG 0.521901 4_1:+51223562 Msa0553940:five_prime_UTR
AAGCTCACCGCAGCTGCCTG+GGG 0.523366 4_1:-51225674 None:intergenic
CTGGTATATTGTATTCATCT+GGG 0.532782 4_1:+51224539 Msa0553940:five_prime_UTR
TGGGATCCTAACTATAGGGT+TGG 0.533189 4_1:-51223638 None:intergenic
ATGTCAAGAAAAGGGTCAGT+TGG 0.533509 4_1:-51224043 None:intergenic
AAACCATACACATGAAACAT+TGG 0.533547 4_1:-51224691 None:intergenic
TCCAAACACGGAGGCAAAAG+TGG 0.534472 4_1:+51225624 Msa0553940:five_prime_UTR
TTCTTGACAACAAATGCCAC+TGG 0.535082 4_1:-51226746 None:intergenic
GAAGCCATGGATTTGACTAA+AGG 0.537715 4_1:-51224157 None:intergenic
ATATCAACAATTGATAAAGA+TGG 0.537763 4_1:+51226286 Msa0553940:five_prime_UTR
ATCATCAATAACTGATCAAG+TGG 0.539714 4_1:+51223082 Msa0553940:five_prime_UTR
GTTACCTTTAGTCAAATCCA+TGG 0.539728 4_1:+51224153 Msa0553940:five_prime_UTR
AGATCCTCCGAAAGCAACTC+AGG 0.540928 4_1:+51227003 Msa0553940:CDS
AAAGTGGTAGACTGGAACAA+TGG 0.541163 4_1:+51225640 Msa0553940:five_prime_UTR
CTCAGGATACTTGAATAATG+AGG 0.541417 4_1:+51226255 Msa0553940:intron
CACATCTCATTATGTTTGAG+AGG 0.543063 4_1:+51227854 Msa0553940:three_prime_UTR
TTCATATACTTACGCTGTAA+CGG 0.543086 4_1:-51226575 None:intergenic
AATGTTAACGTGTACCCTTA+AGG 0.543830 4_1:+51223215 Msa0553940:five_prime_UTR
CTACATGAGACTTATGGTAA+TGG 0.544122 4_1:-51227930 None:intergenic
AATCAACGTTAGGGAAAGCA+TGG 0.550452 4_1:-51224957 None:intergenic
GAAAGACATTATCAAATACA+AGG 0.550958 4_1:+51226375 Msa0553940:five_prime_UTR
GGTCGATTATGTTGCTGAGC+AGG 0.550976 4_1:+51226799 Msa0553940:CDS
GTGGAAATGGGGATATGAAA+GGG 0.551077 4_1:-51223749 None:intergenic
ATTTAACTCATTAACTTGTG+CGG 0.552135 4_1:-51223243 None:intergenic
GGGACTACAGGTGTGAGTAA+AGG 0.553349 4_1:+51224559 Msa0553940:five_prime_UTR
TTATAGGATATTGGATCAGA+GGG 0.554300 4_1:+51227820 Msa0553940:three_prime_UTR
TTTGTGTAAAGCAGAAGCCA+TGG 0.560434 4_1:-51224170 None:intergenic
CCTGTATAAAATCAACGTTA+GGG 0.562718 4_1:-51224966 None:intergenic
CTTGAAACAATCAATTCTGA+GGG 0.563240 4_1:+51227660 Msa0553940:three_prime_UTR
TGACATTGACGAGGTTATCA+GGG 0.564262 4_1:+51224774 Msa0553940:five_prime_UTR
ACACTACTAAGAGGATTCAA+TGG 0.565007 4_1:-51224295 None:intergenic
ACTTGAAACAATCAATTCTG+AGG 0.568297 4_1:+51227659 Msa0553940:three_prime_UTR
CAACAATTGATAAAGATGGT+TGG 0.569594 4_1:+51226290 Msa0553940:five_prime_UTR
GGCATTGACAATGAAGGCAA+AGG 0.569984 4_1:+51224849 Msa0553940:five_prime_UTR
GCATTGACAATGAAGGCAAA+GGG 0.570042 4_1:+51224850 Msa0553940:five_prime_UTR
AACGAATTTCCAGAAAGTCA+TGG 0.570633 4_1:+51223149 Msa0553940:five_prime_UTR
ATTTGATAATGTCTTTCAAG+CGG 0.579224 4_1:-51226369 None:intergenic
CTAATAACTCCTATGCTCAA+AGG 0.579398 4_1:-51224925 None:intergenic
ATTATAGGATATTGGATCAG+AGG 0.579489 4_1:+51227819 Msa0553940:three_prime_UTR
ATTGTCAATGCCGACAACAT+CGG 0.579863 4_1:-51224838 None:intergenic
GGACTATAATCATAATGTTG+GGG 0.580599 4_1:-51223706 None:intergenic
TGTGGAAATGGGGATATGAA+AGG 0.582479 4_1:-51223750 None:intergenic
TGAATTTCAAGAAAAGAGAA+CGG 0.583620 4_1:-51223894 None:intergenic
CAGTTGGAAGATTTCTAGGA+GGG 0.583658 4_1:-51224027 None:intergenic
GTTCAAATTAGCAAAGGTGC+AGG 0.584639 4_1:+51227748 Msa0553940:three_prime_UTR
GGTGCAGCACCTTTGAGCAT+AGG 0.585948 4_1:+51224916 Msa0553940:five_prime_UTR
TGCTGTGCCAGAAAATGAGA+AGG 0.586565 4_1:+51224423 Msa0553940:five_prime_UTR
TAACTGACCAAAGGTGTACA+GGG 0.586877 4_1:-51227310 None:intergenic
AGGCTGTGGCTTGAGTGTGA+AGG 0.586939 4_1:-51223782 None:intergenic
GTGGTGTAAAATGTTGAATG+TGG 0.591694 4_1:-51223867 None:intergenic
AGTTTGAGACAGGTCTCTTG+CGG 0.592614 4_1:+51224895 Msa0553940:five_prime_UTR
GATGAATACCAATGAGGGTG+AGG 0.592922 4_1:-51223977 None:intergenic
GTTGTCGGCATTGACAATGA+AGG 0.595731 4_1:+51224843 Msa0553940:five_prime_UTR
TGCCGAAGATGAAGAAATTG+GGG 0.602629 4_1:+51226718 Msa0553940:CDS
AAGATACCAAGGTCTCCGAC+TGG 0.604082 4_1:+51226978 Msa0553940:CDS
ATTAAACAGGAAGATACTGC+TGG 0.608838 4_1:+51224520 Msa0553940:five_prime_UTR
CGTAAACAGGTTCAAGAGTG+TGG 0.611754 4_1:+51224328 Msa0553940:five_prime_UTR
AGGGTTGTTGGCTCCAAACA+CGG 0.614077 4_1:+51225612 Msa0553940:five_prime_UTR
TACCAAAGAAAGACAAGACA+TGG 0.614263 4_1:+51227081 Msa0553940:three_prime_UTR
AAAGACATTATCAAATACAA+GGG 0.615550 4_1:+51226376 Msa0553940:five_prime_UTR
GTCAATGCCGACAACATCGG+TGG 0.615987 4_1:-51224835 None:intergenic
TTTCGGAGGATCTTCCCAGT+CGG 0.617027 4_1:-51226993 None:intergenic
AGAGGGCCTTCTATTATGAG+AGG 0.618979 4_1:+51225703 Msa0553940:five_prime_UTR
AGATACCAAGGTCTCCGACT+GGG 0.620788 4_1:+51226979 Msa0553940:CDS
AAAGAAAGACAAGACATGGC+GGG 0.620901 4_1:+51227085 Msa0553940:three_prime_UTR
GCAGGTTGCTCCATACAAGA+AGG 0.622353 4_1:+51226932 Msa0553940:intron
TCGACTGCAGTGGGAACACG+TGG 0.624008 4_1:+51225553 Msa0553940:five_prime_UTR
AACTGCGTTCAAATTAGCAA+AGG 0.630302 4_1:+51227742 Msa0553940:three_prime_UTR
GCTCTACGGTCATTGTAATG+AGG 0.634594 4_1:+51224746 Msa0553940:five_prime_UTR
AATACTTGGAAAATTTCTGT+CGG 0.635384 4_1:+51227716 Msa0553940:three_prime_UTR
GCACTTTAGAAGATTACGGA+TGG 0.635858 4_1:-51226642 None:intergenic
TTACCATAACATGAAACTGG+TGG 0.637363 4_1:+51227108 Msa0553940:three_prime_UTR
CTCTTACTATAACTGACCAA+AGG 0.640497 4_1:-51227319 None:intergenic
AGCTCACCGCAGCTGCCTGG+GGG 0.641341 4_1:-51225673 None:intergenic
AGGGCTAAAACCCCATCATG+AGG 0.644178 4_1:-51224088 None:intergenic
AGTGCCTGAGTTGCTTTCGG+AGG 0.646420 4_1:-51227007 None:intergenic
ATGACTGAGTCGACTGCAGT+GGG 0.649133 4_1:+51225544 Msa0553940:five_prime_UTR
TTATAATCAAATGTCCAAAG+AGG 0.651413 4_1:-51226664 None:intergenic
TCCATACAAGAAGGTCCGGA+AGG 0.659172 4_1:+51226941 Msa0553940:CDS
TCAGTTGGAAGATTTCTAGG+AGG 0.666964 4_1:-51224028 None:intergenic
TGTACTTTCTCCGAAGCATG+TGG 0.668913 4_1:+51226778 Msa0553940:CDS
AATGACTGAGTCGACTGCAG+TGG 0.680151 4_1:+51225543 Msa0553940:five_prime_UTR
AAGAAACTAGAGCTATTAGG+TGG 0.680245 4_1:+51224385 Msa0553940:five_prime_UTR
GAGGAAATTTGACATTGACG+AGG 0.683873 4_1:+51224765 Msa0553940:five_prime_UTR
ATGAATACCAATGAGGGTGA+GGG 0.691317 4_1:-51223976 None:intergenic
AGAAATTGGGGAAATTCCAG+TGG 0.696561 4_1:+51226730 Msa0553940:CDS
CAAAGAAAGACAAGACATGG+CGG 0.716272 4_1:+51227084 Msa0553940:three_prime_UTR
GAACAGCCAAGAGTATAACA+GGG 0.749221 4_1:-51224249 None:intergenic
TCTTCACCGACAAGATACCA+AGG 0.749835 4_1:+51226967 Msa0553940:CDS
GTTGTTGGCTCCAAACACGG+AGG 0.795141 4_1:+51225615 Msa0553940:five_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAATTAAATTCAATGCA+CGG - chr4_1:51223290-51223309 None:intergenic 15.0%
!! TAATATCCAAAATTCATTAT+TGG - chr4_1:51223486-51223505 None:intergenic 15.0%
!!! GGATATTAGTTTTTTTTTTT+TGG + chr4_1:51223498-51223517 Msa0553940:five_prime_UTR 15.0%
!! AAAATAGTGAAATAGAAAAA+GGG - chr4_1:51223822-51223841 None:intergenic 15.0%
!! TAAAATAGTGAAATAGAAAA+AGG - chr4_1:51223823-51223842 None:intergenic 15.0%
!!! TTATTAACATTTTACTGATA+AGG - chr4_1:51226016-51226035 None:intergenic 15.0%
!!! GTTTGATTAACAATTTTAAT+TGG - chr4_1:51226221-51226240 None:intergenic 15.0%
!! CAGAAGAAAAAAAAAATAAA+GGG - chr4_1:51226912-51226931 None:intergenic 15.0%
!!! GTAATATTTTCATGAATTAT+AGG + chr4_1:51227804-51227823 Msa0553940:three_prime_UTR 15.0%
!! AACAATAACTAAAAACTCTT+TGG + chr4_1:51223190-51223209 Msa0553940:five_prime_UTR 20.0%
!! ACAATAACTAAAAACTCTTT+GGG + chr4_1:51223191-51223210 Msa0553940:five_prime_UTR 20.0%
!!! TATTTTTGTGTGTGAAAAAA+GGG - chr4_1:51223460-51223479 None:intergenic 20.0%
!!! ATATTTTTGTGTGTGAAAAA+AGG - chr4_1:51223461-51223480 None:intergenic 20.0%
!!! ATATGACCAATAATGAATTT+TGG + chr4_1:51223477-51223496 Msa0553940:five_prime_UTR 20.0%
!! CAAAAAATTAAACAAAACCA+AGG - chr4_1:51223546-51223565 None:intergenic 20.0%
!!! AGTTTTTTTAACCTTCTTTA+TGG + chr4_1:51224469-51224488 Msa0553940:five_prime_UTR 20.0%
!! AATCAAATTTACCATAAAGA+AGG - chr4_1:51224483-51224502 None:intergenic 20.0%
!!! TCATAGAAATCTTATTTCTA+TGG + chr4_1:51224594-51224613 Msa0553940:five_prime_UTR 20.0%
!! ATTAAGACTATTCTATTCAA+AGG - chr4_1:51224999-51225018 None:intergenic 20.0%
!! TTTAATAGCAACTGTTATAT+AGG + chr4_1:51225137-51225156 Msa0553940:intron 20.0%
!! TTAATAGCAACTGTTATATA+GGG + chr4_1:51225138-51225157 Msa0553940:intron 20.0%
!!! CAAATAAAGTTCAACTTTTA+TGG - chr4_1:51225455-51225474 None:intergenic 20.0%
!! TAGATATTAAAAGTGAAACA+CGG + chr4_1:51225749-51225768 Msa0553940:intron 20.0%
!! ATATAGAGCAAATATAGTAA+AGG - chr4_1:51225844-51225863 None:intergenic 20.0%
!! ATATCAACAATTGATAAAGA+TGG + chr4_1:51226286-51226305 Msa0553940:five_prime_UTR 20.0%
!!! AGATATTGTTTATTTTGATC+AGG + chr4_1:51226324-51226343 Msa0553940:five_prime_UTR 20.0%
!! AAAGACATTATCAAATACAA+GGG + chr4_1:51226376-51226395 Msa0553940:five_prime_UTR 20.0%
!! CCAGAAGAAAAAAAAAATAA+AGG - chr4_1:51226913-51226932 None:intergenic 20.0%
!!! CCTTTATTTTTTTTTTCTTC+TGG + chr4_1:51226910-51226929 Msa0553940:intron 20.0%
!!! ATCATTTGTTTTGATTACTT+GGG - chr4_1:51227575-51227594 None:intergenic 20.0%
!!! TATCATTTGTTTTGATTACT+TGG - chr4_1:51227576-51227595 None:intergenic 20.0%
!! TTTCATGAATTATAGGATAT+TGG + chr4_1:51227811-51227830 Msa0553940:three_prime_UTR 20.0%
!! CTAAATACCTCTAAAAAAAG+TGG + chr4_1:51223110-51223129 Msa0553940:five_prime_UTR 25.0%
!!! ATTTAACTCATTAACTTGTG+CGG - chr4_1:51223246-51223265 None:intergenic 25.0%
! GAGTTTGATTAAAAGAGTTT+GGG - chr4_1:51223397-51223416 None:intergenic 25.0%
! CTCCTAAAATATAACTCTTT+AGG + chr4_1:51223414-51223433 Msa0553940:five_prime_UTR 25.0%
!! GACCTAAAGAGTTATATTTT+AGG - chr4_1:51223419-51223438 None:intergenic 25.0%
! TTAAACAAAACCAAGGAAAA+GGG - chr4_1:51223539-51223558 None:intergenic 25.0%
! ATTAAACAAAACCAAGGAAA+AGG - chr4_1:51223540-51223559 None:intergenic 25.0%
!!! ATATTTGGTGTGAAGAAAAA+GGG - chr4_1:51223679-51223698 None:intergenic 25.0%
!!! AATATTTGGTGTGAAGAAAA+AGG - chr4_1:51223680-51223699 None:intergenic 25.0%
! TGAATTTCAAGAAAAGAGAA+CGG - chr4_1:51223897-51223916 None:intergenic 25.0%
!! TCTCTTTTCTTGAAATTCAA+TGG + chr4_1:51223898-51223917 Msa0553940:five_prime_UTR 25.0%
!! TTTTTGATGAATACCAATGA+GGG - chr4_1:51223985-51224004 None:intergenic 25.0%
! AAGAAACAATGTCAAGAAAA+GGG - chr4_1:51224054-51224073 None:intergenic 25.0%
! AAAGAAACAATGTCAAGAAA+AGG - chr4_1:51224055-51224074 None:intergenic 25.0%
! TAACAACTTTGAGTAAGAAA+TGG - chr4_1:51224137-51224156 None:intergenic 25.0%
! ACAGGGTAATAAATTGAATT+TGG - chr4_1:51224235-51224254 None:intergenic 25.0%
!!! TAAATTTGATTTGCCTCAAA+GGG + chr4_1:51224492-51224511 Msa0553940:five_prime_UTR 25.0%
! AATAAATTGTTTAGCCAAAC+AGG - chr4_1:51225164-51225183 None:intergenic 25.0%
! AAATAAGCTGAAAAGAACTA+AGG + chr4_1:51225314-51225333 Msa0553940:intron 25.0%
!! TGTAAGATTGTTTCCTTTTA+GGG + chr4_1:51225514-51225533 Msa0553940:intron 25.0%
! ATATAAAGGTCTTGATTCAA+TGG + chr4_1:51225777-51225796 Msa0553940:intron 25.0%
!! ATTTGTTAACGCTTAACTAA+AGG + chr4_1:51225862-51225881 Msa0553940:intron 25.0%
! AACTAACATGATATTTCTCA+AGG - chr4_1:51225895-51225914 None:intergenic 25.0%
! AAACTGAAGTAAATTTCTCT+TGG + chr4_1:51226103-51226122 Msa0553940:intron 25.0%
!!! ATTGTTTATTTTGATCAGGA+TGG + chr4_1:51226328-51226347 Msa0553940:five_prime_UTR 25.0%
!!! ATTTGATAATGTCTTTCAAG+CGG - chr4_1:51226372-51226391 None:intergenic 25.0%
! GAAAGACATTATCAAATACA+AGG + chr4_1:51226375-51226394 Msa0553940:five_prime_UTR 25.0%
! TTATAATCAAATGTCCAAAG+AGG - chr4_1:51226667-51226686 None:intergenic 25.0%
!!! TATTTTTTTTTTCTTCTGGC+AGG + chr4_1:51226914-51226933 Msa0553940:intron 25.0%
!! TCATGAGTACATCTTTTTAA+AGG + chr4_1:51227155-51227174 Msa0553940:three_prime_UTR 25.0%
!! AATGTGCAGAATAGAAATTT+TGG - chr4_1:51227213-51227232 None:intergenic 25.0%
! TTCACTTGTAATCTTTGATA+AGG + chr4_1:51227502-51227521 Msa0553940:three_prime_UTR 25.0%
! TTAATACCTTATTCTTCTTC+AGG + chr4_1:51227538-51227557 Msa0553940:three_prime_UTR 25.0%
! CAGTAATAACAAAATCATTC+AGG + chr4_1:51227599-51227618 Msa0553940:three_prime_UTR 25.0%
! AATACTTGGAAAATTTCTGT+CGG + chr4_1:51227716-51227735 Msa0553940:three_prime_UTR 25.0%
! ACTTTCAACAAAAAGACATT+TGG + chr4_1:51227782-51227801 Msa0553940:three_prime_UTR 25.0%
!!! TAACATGATTTTAGCTAAGA+AGG + chr4_1:51227894-51227913 Msa0553940:three_prime_UTR 25.0%
!! AATTGTATTCTTTTGTCTTC+TGG - chr4_1:51228010-51228029 None:intergenic 25.0%
ATCATCAATAACTGATCAAG+TGG + chr4_1:51223082-51223101 Msa0553940:five_prime_UTR 30.0%
!!! AGACTAACCACTTTTTTTAG+AGG - chr4_1:51223120-51223139 None:intergenic 30.0%
TAAATATCACCATGACTTTC+TGG - chr4_1:51223161-51223180 None:intergenic 30.0%
GGAGTTTGATTAAAAGAGTT+TGG - chr4_1:51223398-51223417 None:intergenic 30.0%
!! TATTTTTATGCCCTTTTCCT+TGG + chr4_1:51223526-51223545 Msa0553940:five_prime_UTR 30.0%
! TTTTGTCTTTATCATGTGTG+CGG + chr4_1:51223561-51223580 Msa0553940:five_prime_UTR 30.0%
AAATTTCCAACCCTATAGTT+AGG + chr4_1:51223632-51223651 Msa0553940:five_prime_UTR 30.0%
TAATCATAATGTTGGGGATT+TGG - chr4_1:51223703-51223722 None:intergenic 30.0%
GGACTATAATCATAATGTTG+GGG - chr4_1:51223709-51223728 None:intergenic 30.0%
GGGACTATAATCATAATGTT+GGG - chr4_1:51223710-51223729 None:intergenic 30.0%
AGGGACTATAATCATAATGT+TGG - chr4_1:51223711-51223730 None:intergenic 30.0%
!!! TTGAGTGATCATGTTGTTTT+GGG - chr4_1:51223954-51223973 None:intergenic 30.0%
! GTTTTTGATGAATACCAATG+AGG - chr4_1:51223986-51224005 None:intergenic 30.0%
CATTGGTATTCATCAAAAAC+TGG + chr4_1:51223986-51224005 Msa0553940:five_prime_UTR 30.0%
!!! ATTTTTTCACATCCTCATGA+TGG + chr4_1:51224076-51224095 Msa0553940:five_prime_UTR 30.0%
!!! TTTTTTCACATCCTCATGAT+GGG + chr4_1:51224077-51224096 Msa0553940:five_prime_UTR 30.0%
TTATTACCCTGTTATACTCT+TGG + chr4_1:51224243-51224262 Msa0553940:five_prime_UTR 30.0%
!! TCTAGTTTCTTGACATTTTC+AGG - chr4_1:51224376-51224395 None:intergenic 30.0%
!! GTAAATTTGATTTGCCTCAA+AGG + chr4_1:51224491-51224510 Msa0553940:five_prime_UTR 30.0%
TGTTTAATAACTACCCTTTG+AGG - chr4_1:51224508-51224527 None:intergenic 30.0%
TCAAAGGGTAGTTATTAAAC+AGG + chr4_1:51224507-51224526 Msa0553940:five_prime_UTR 30.0%
CTGGTATATTGTATTCATCT+GGG + chr4_1:51224539-51224558 Msa0553940:five_prime_UTR 30.0%
AAACCATACACATGAAACAT+TGG - chr4_1:51224694-51224713 None:intergenic 30.0%
CCTGTATAAAATCAACGTTA+GGG - chr4_1:51224969-51224988 None:intergenic 30.0%
ACCTGTATAAAATCAACGTT+AGG - chr4_1:51224970-51224989 None:intergenic 30.0%
TCAAGCAATCATATAAACGA+TGG - chr4_1:51225042-51225061 None:intergenic 30.0%
!! TTATGGTCTAGAATTTGTTG+TGG - chr4_1:51225438-51225457 None:intergenic 30.0%
! CTGTAAGATTGTTTCCTTTT+AGG + chr4_1:51225513-51225532 Msa0553940:intron 30.0%
!!! ATTGTTTCCTTTTAGGGTTA+TGG + chr4_1:51225520-51225539 Msa0553940:intron 30.0%
TTTCACAACTATTCGTCAAT+AGG + chr4_1:51225592-51225611 Msa0553940:five_prime_UTR 30.0%
TTCACAACTATTCGTCAATA+GGG + chr4_1:51225593-51225612 Msa0553940:five_prime_UTR 30.0%
TTCTTACCTCTCATAATAGA+AGG - chr4_1:51225712-51225731 None:intergenic 30.0%
GAAACACGGTAGTAATATAA+AGG + chr4_1:51225763-51225782 Msa0553940:intron 30.0%
AACTCTTTCCTTTAATTCTC+AGG + chr4_1:51226238-51226257 Msa0553940:intron 30.0%
TCAAGTATCCTGAGAATTAA+AGG - chr4_1:51226249-51226268 None:intergenic 30.0%
CAACAATTGATAAAGATGGT+TGG + chr4_1:51226290-51226309 Msa0553940:five_prime_UTR 30.0%
TCAGGATGGATATTTACATT+TGG + chr4_1:51226342-51226361 Msa0553940:five_prime_UTR 30.0%
TATCAAATACAAGGGATTTC+AGG + chr4_1:51226384-51226403 Msa0553940:five_prime_UTR 30.0%
!! GATTTCAGGTATCTACTTTT+AGG + chr4_1:51226398-51226417 Msa0553940:intron 30.0%
TTCATATACTTACGCTGTAA+CGG - chr4_1:51226578-51226597 None:intergenic 30.0%
GAAAAATGACGTAACATTTG+AGG - chr4_1:51226690-51226709 None:intergenic 30.0%
TAGAAAGTTCAAAAACCAGA+AGG + chr4_1:51227264-51227283 Msa0553940:three_prime_UTR 30.0%
!!! TTGATGGTTTTTTTTCCTTC+TGG - chr4_1:51227282-51227301 None:intergenic 30.0%
ATTTCTCTAGTGCATTTACA+AGG - chr4_1:51227347-51227366 None:intergenic 30.0%
! ATTGAAAAGTATGACACAAC+AGG - chr4_1:51227448-51227467 None:intergenic 30.0%
! TGTCATACTTTTCAATTCAG+CGG + chr4_1:51227452-51227471 Msa0553940:three_prime_UTR 30.0%
! AATTCAGCGGTTAAGATTTT+TGG + chr4_1:51227465-51227484 Msa0553940:three_prime_UTR 30.0%
GCAGTGTAATAAAAAGAACA+AGG + chr4_1:51227627-51227646 Msa0553940:three_prime_UTR 30.0%
ACTTGAAACAATCAATTCTG+AGG + chr4_1:51227659-51227678 Msa0553940:three_prime_UTR 30.0%
CTTGAAACAATCAATTCTGA+GGG + chr4_1:51227660-51227679 Msa0553940:three_prime_UTR 30.0%
!! TTTGTTGAAAGTGATGGATT+CGG - chr4_1:51227774-51227793 None:intergenic 30.0%
! TGTCTTTTTGTTGAAAGTGA+TGG - chr4_1:51227780-51227799 None:intergenic 30.0%
ATTATAGGATATTGGATCAG+AGG + chr4_1:51227819-51227838 Msa0553940:three_prime_UTR 30.0%
TTATAGGATATTGGATCAGA+GGG + chr4_1:51227820-51227839 Msa0553940:three_prime_UTR 30.0%
AACGAATTTCCAGAAAGTCA+TGG + chr4_1:51223149-51223168 Msa0553940:five_prime_UTR 35.0%
AATGTTAACGTGTACCCTTA+AGG + chr4_1:51223215-51223234 Msa0553940:five_prime_UTR 35.0%
TTGTGTGTGAAAAAAGGGTT+TGG - chr4_1:51223455-51223474 None:intergenic 35.0%
TTTGTCTTTATCATGTGTGC+GGG + chr4_1:51223562-51223581 Msa0553940:five_prime_UTR 35.0%
TGGAAATTTCTGTACGCATT+TGG - chr4_1:51223621-51223640 None:intergenic 35.0%
TTGTTGGGATCCTAACTATA+GGG - chr4_1:51223645-51223664 None:intergenic 35.0%
TTTGTTGGGATCCTAACTAT+AGG - chr4_1:51223646-51223665 None:intergenic 35.0%
AGGGTGAAATTGTCTTTGTT+GGG - chr4_1:51223660-51223679 None:intergenic 35.0%
AAGGGTGAAATTGTCTTTGT+TGG - chr4_1:51223661-51223680 None:intergenic 35.0%
! TGTTGGGGATTTGGAATATT+TGG - chr4_1:51223694-51223713 None:intergenic 35.0%
GAACGTAATAGCTACGTTTA+GGG - chr4_1:51223730-51223749 None:intergenic 35.0%
! GTTTTGTGTTTGTGGAAATG+GGG - chr4_1:51223763-51223782 None:intergenic 35.0%
!! GGTTTTGTGTTTGTGGAAAT+GGG - chr4_1:51223764-51223783 None:intergenic 35.0%
!! AGGTTTTGTGTTTGTGGAAA+TGG - chr4_1:51223765-51223784 None:intergenic 35.0%
!!! AAAGGGTGTGTGTTTTTTTG+AGG - chr4_1:51223805-51223824 None:intergenic 35.0%
GTGGTGTAAAATGTTGAATG+TGG - chr4_1:51223870-51223889 None:intergenic 35.0%
! AAGAAAAGAGAACGGTTTTG+TGG - chr4_1:51223889-51223908 None:intergenic 35.0%
!! GTTGAGTGATCATGTTGTTT+TGG - chr4_1:51223955-51223974 None:intergenic 35.0%
! TTTTTCACATCCTCATGATG+GGG + chr4_1:51224078-51224097 Msa0553940:five_prime_UTR 35.0%
CCCAGATGAAGAATCAATAA+GGG - chr4_1:51224110-51224129 None:intergenic 35.0%
!! CCCTTATTGATTCTTCATCT+GGG + chr4_1:51224107-51224126 Msa0553940:five_prime_UTR 35.0%
ACCCAGATGAAGAATCAATA+AGG - chr4_1:51224111-51224130 None:intergenic 35.0%
GTTACCTTTAGTCAAATCCA+TGG + chr4_1:51224153-51224172 Msa0553940:five_prime_UTR 35.0%
GCTTCTGCTTTACACAAAAT+GGG + chr4_1:51224175-51224194 Msa0553940:five_prime_UTR 35.0%
ACACTACTAAGAGGATTCAA+TGG - chr4_1:51224298-51224317 None:intergenic 35.0%
TTGAATCCTCTTAGTAGTGT+TGG + chr4_1:51224298-51224317 Msa0553940:five_prime_UTR 35.0%
ATTTCACCAACACTACTAAG+AGG - chr4_1:51224307-51224326 None:intergenic 35.0%
! TGTTGGTGAAATTCGTAAAC+AGG + chr4_1:51224315-51224334 Msa0553940:five_prime_UTR 35.0%
GTCAAGAAACTAGAGCTATT+AGG + chr4_1:51224382-51224401 Msa0553940:five_prime_UTR 35.0%
AAGAAACTAGAGCTATTAGG+TGG + chr4_1:51224385-51224404 Msa0553940:five_prime_UTR 35.0%
!! TTTTCTGGCACAGCAATAAT+GGG - chr4_1:51224418-51224437 None:intergenic 35.0%
!! ATTTTCTGGCACAGCAATAA+TGG - chr4_1:51224419-51224438 None:intergenic 35.0%
! CTCAAATCCTTCTCATTTTC+TGG - chr4_1:51224433-51224452 None:intergenic 35.0%
CAGAAAATGAGAAGGATTTG+AGG + chr4_1:51224431-51224450 Msa0553940:five_prime_UTR 35.0%
ATTAAACAGGAAGATACTGC+TGG + chr4_1:51224520-51224539 Msa0553940:five_prime_UTR 35.0%
GCTGGTATATTGTATTCATC+TGG + chr4_1:51224538-51224557 Msa0553940:five_prime_UTR 35.0%
TTGCCAATGTTTCATGTGTA+TGG + chr4_1:51224688-51224707 Msa0553940:five_prime_UTR 35.0%
CTAATAACTCCTATGCTCAA+AGG - chr4_1:51224928-51224947 None:intergenic 35.0%
TTGAGCATAGGAGTTATTAG+TGG + chr4_1:51224928-51224947 Msa0553940:five_prime_UTR 35.0%
!!! CCCTAACGTTGATTTTATAC+AGG + chr4_1:51224966-51224985 Msa0553940:five_prime_UTR 35.0%
!! GTCGTTTTCATAAGCTATCT+TGG + chr4_1:51225280-51225299 Msa0553940:intron 35.0%
! TAAGTTTTGCGAGACTGTTT+GGG - chr4_1:51225370-51225389 None:intergenic 35.0%
! ATAAGTTTTGCGAGACTGTT+TGG - chr4_1:51225371-51225390 None:intergenic 35.0%
AAACAGTCTCGCAAAACTTA+TGG + chr4_1:51225370-51225389 Msa0553940:intron 35.0%
AGTCATTCCATAACCCTAAA+AGG - chr4_1:51225530-51225549 None:intergenic 35.0%
ATGATATTTCTCAAGGACAC+AGG - chr4_1:51225888-51225907 None:intergenic 35.0%
! TTTTCCCACCTAGTGAAAAA+AGG - chr4_1:51226086-51226105 None:intergenic 35.0%
! AGAAAAAAGAAGGTACAGGT+AGG - chr4_1:51226180-51226199 None:intergenic 35.0%
! GACAAGAAAAAAGAAGGTAC+AGG - chr4_1:51226184-51226203 None:intergenic 35.0%
CAGCAAGACAAGAAAAAAGA+AGG - chr4_1:51226190-51226209 None:intergenic 35.0%
CTCAGGATACTTGAATAATG+AGG + chr4_1:51226255-51226274 Msa0553940:intron 35.0%
!! AGAGGCACTTTAGAAGATTA+CGG - chr4_1:51226649-51226668 None:intergenic 35.0%
!! AATCTTCTAAAGTGCCTCTT+TGG + chr4_1:51226650-51226669 Msa0553940:CDS 35.0%
TTCCCCAATTTCTTCATCTT+CGG - chr4_1:51226723-51226742 None:intergenic 35.0%
!! TGCTTGAGTTCTAAACTCTA+AGG + chr4_1:51227029-51227048 Msa0553940:CDS 35.0%
TACCAAAGAAAGACAAGACA+TGG + chr4_1:51227081-51227100 Msa0553940:three_prime_UTR 35.0%
TTACCATAACATGAAACTGG+TGG + chr4_1:51227108-51227127 Msa0553940:three_prime_UTR 35.0%
CTCTTACTATAACTGACCAA+AGG - chr4_1:51227322-51227341 None:intergenic 35.0%
!!! TTAAGATTTTTGGTGGTCGA+AGG + chr4_1:51227475-51227494 Msa0553940:three_prime_UTR 35.0%
TCTTTGCCTGAAGAAGAATA+AGG - chr4_1:51227547-51227566 None:intergenic 35.0%
ACATCTGAATTATGAGTCGT+TGG + chr4_1:51227688-51227707 Msa0553940:three_prime_UTR 35.0%
AACTGCGTTCAAATTAGCAA+AGG + chr4_1:51227742-51227761 Msa0553940:three_prime_UTR 35.0%
CACATCTCATTATGTTTGAG+AGG + chr4_1:51227854-51227873 Msa0553940:three_prime_UTR 35.0%
CTACATGAGACTTATGGTAA+TGG - chr4_1:51227933-51227952 None:intergenic 35.0%
ACCATAAGTCTCATGTAGAT+TGG + chr4_1:51227935-51227954 Msa0553940:three_prime_UTR 35.0%
CCATAAGTCTCATGTAGATT+GGG + chr4_1:51227936-51227955 Msa0553940:three_prime_UTR 35.0%
GGAACGTAATAGCTACGTTT+AGG - chr4_1:51223731-51223750 None:intergenic 40.0%
GTGGAAATGGGGATATGAAA+GGG - chr4_1:51223752-51223771 None:intergenic 40.0%
TGTGGAAATGGGGATATGAA+AGG - chr4_1:51223753-51223772 None:intergenic 40.0%
!! GTGTGAAGGTTTTGTGTTTG+TGG - chr4_1:51223771-51223790 None:intergenic 40.0%
!!! TGTGTGTTTTTTTGAGGCTG+TGG - chr4_1:51223799-51223818 None:intergenic 40.0%
ATGAATACCAATGAGGGTGA+GGG - chr4_1:51223979-51223998 None:intergenic 40.0%
! CAGTTGGAAGATTTCTAGGA+GGG - chr4_1:51224030-51224049 None:intergenic 40.0%
! TCAGTTGGAAGATTTCTAGG+AGG - chr4_1:51224031-51224050 None:intergenic 40.0%
ATGTCAAGAAAAGGGTCAGT+TGG - chr4_1:51224046-51224065 None:intergenic 40.0%
!! GCCCTTATTGATTCTTCATC+TGG + chr4_1:51224106-51224125 Msa0553940:five_prime_UTR 40.0%
GAAGCCATGGATTTGACTAA+AGG - chr4_1:51224160-51224179 None:intergenic 40.0%
TTTGTGTAAAGCAGAAGCCA+TGG - chr4_1:51224173-51224192 None:intergenic 40.0%
GGCTTCTGCTTTACACAAAA+TGG + chr4_1:51224174-51224193 Msa0553940:five_prime_UTR 40.0%
CACAAAATGGGTGTTTCACA+AGG + chr4_1:51224187-51224206 Msa0553940:five_prime_UTR 40.0%
! GAACAGCCAAGAGTATAACA+GGG - chr4_1:51224252-51224271 None:intergenic 40.0%
! AGAACAGCCAAGAGTATAAC+AGG - chr4_1:51224253-51224272 None:intergenic 40.0%
CTCTTGGCTGTTCTGTATTT+AGG + chr4_1:51224259-51224278 Msa0553940:five_prime_UTR 40.0%
! TTGTATTCATCTGGGACTAC+AGG + chr4_1:51224547-51224566 Msa0553940:five_prime_UTR 40.0%
AACAGCTAGAAACACACTCT+TGG - chr4_1:51224668-51224687 None:intergenic 40.0%
ATTATTGTCGTTGGGCTCTA+CGG + chr4_1:51224732-51224751 Msa0553940:five_prime_UTR 40.0%
GAGGAAATTTGACATTGACG+AGG + chr4_1:51224765-51224784 Msa0553940:five_prime_UTR 40.0%
TTGACATTGACGAGGTTATC+AGG + chr4_1:51224773-51224792 Msa0553940:five_prime_UTR 40.0%
TGACATTGACGAGGTTATCA+GGG + chr4_1:51224774-51224793 Msa0553940:five_prime_UTR 40.0%
ATTGTCAATGCCGACAACAT+CGG - chr4_1:51224841-51224860 None:intergenic 40.0%
! GCATTGACAATGAAGGCAAA+GGG + chr4_1:51224850-51224869 Msa0553940:five_prime_UTR 40.0%
!! ATGAAGGCAAAGGGTGTTAA+TGG + chr4_1:51224859-51224878 Msa0553940:five_prime_UTR 40.0%
! TAAGTTGCAGAGTTTGAGAC+AGG + chr4_1:51224885-51224904 Msa0553940:five_prime_UTR 40.0%
AATCAACGTTAGGGAAAGCA+TGG - chr4_1:51224960-51224979 None:intergenic 40.0%
ACTGTTATATAGGGCCTGTT+TGG + chr4_1:51225147-51225166 Msa0553940:intron 40.0%
GTTGTGGATGTGAACATCAA+TGG - chr4_1:51225422-51225441 None:intergenic 40.0%
CTATTCGTCAATAGGGTTGT+TGG + chr4_1:51225600-51225619 Msa0553940:five_prime_UTR 40.0%
AAAGTGGTAGACTGGAACAA+TGG + chr4_1:51225640-51225659 Msa0553940:five_prime_UTR 40.0%
!!! AATGGTACTTTTTTACCCCC+AGG + chr4_1:51225658-51225677 Msa0553940:five_prime_UTR 40.0%
AAAGGTCTTGATTCAATGGC+CGG + chr4_1:51225781-51225800 Msa0553940:intron 40.0%
ATAGCGAACAACAACAGTCA+AGG + chr4_1:51226060-51226079 Msa0553940:intron 40.0%
!!! AGTCAAGGCCTTTTTTCACT+AGG + chr4_1:51226075-51226094 Msa0553940:intron 40.0%
!!! AAGGCCTTTTTTCACTAGGT+GGG + chr4_1:51226079-51226098 Msa0553940:intron 40.0%
TACAGGTAGGCTAAGGAATA+AGG - chr4_1:51226167-51226186 None:intergenic 40.0%
! AAAGATGGTTGGCTACATAC+AGG + chr4_1:51226301-51226320 Msa0553940:five_prime_UTR 40.0%
! GCACTTTAGAAGATTACGGA+TGG - chr4_1:51226645-51226664 None:intergenic 40.0%
! AGTGCCGAAGATGAAGAAAT+TGG + chr4_1:51226716-51226735 Msa0553940:CDS 40.0%
GTGCCGAAGATGAAGAAATT+GGG + chr4_1:51226717-51226736 Msa0553940:CDS 40.0%
TGCCGAAGATGAAGAAATTG+GGG + chr4_1:51226718-51226737 Msa0553940:CDS 40.0%
AGAAATTGGGGAAATTCCAG+TGG + chr4_1:51226730-51226749 Msa0553940:CDS 40.0%
TTCTTGACAACAAATGCCAC+TGG - chr4_1:51226749-51226768 None:intergenic 40.0%
!! AGTGGCATTTGTTGTCAAGA+AGG + chr4_1:51226748-51226767 Msa0553940:CDS 40.0%
ACATAATCGACCACATGCTT+CGG - chr4_1:51226791-51226810 None:intergenic 40.0%
! GAGCAGGTTCTTCTTTTGAT+TGG + chr4_1:51226815-51226834 Msa0553940:intron 40.0%
CAAAGAAAGACAAGACATGG+CGG + chr4_1:51227084-51227103 Msa0553940:three_prime_UTR 40.0%
AAAGAAAGACAAGACATGGC+GGG + chr4_1:51227085-51227104 Msa0553940:three_prime_UTR 40.0%
GGGTTACCATAACATGAAAC+TGG + chr4_1:51227105-51227124 Msa0553940:three_prime_UTR 40.0%
AGTCCACCAGTTTCATGTTA+TGG - chr4_1:51227114-51227133 None:intergenic 40.0%
! TAACTGACCAAAGGTGTACA+GGG - chr4_1:51227313-51227332 None:intergenic 40.0%
! ATAACTGACCAAAGGTGTAC+AGG - chr4_1:51227314-51227333 None:intergenic 40.0%
!! TCAGCGGTTAAGATTTTTGG+TGG + chr4_1:51227468-51227487 Msa0553940:three_prime_UTR 40.0%
AGTCGTTGGTGTACAATACT+TGG + chr4_1:51227702-51227721 Msa0553940:three_prime_UTR 40.0%
! GTTCAAATTAGCAAAGGTGC+AGG + chr4_1:51227748-51227767 Msa0553940:three_prime_UTR 40.0%
CCCAATCTACATGAGACTTA+TGG - chr4_1:51227939-51227958 None:intergenic 40.0%
!! ACGCATTTGGCTCTCAAGTT+GGG - chr4_1:51223608-51223627 None:intergenic 45.0%
!! TACGCATTTGGCTCTCAAGT+TGG - chr4_1:51223609-51223628 None:intergenic 45.0%
TGGGATCCTAACTATAGGGT+TGG - chr4_1:51223641-51223660 None:intergenic 45.0%
GATGAATACCAATGAGGGTG+AGG - chr4_1:51223980-51223999 None:intergenic 45.0%
GGGTCAGTTGGAAGATTTCT+AGG - chr4_1:51224034-51224053 None:intergenic 45.0%
CGTAAACAGGTTCAAGAGTG+TGG + chr4_1:51224328-51224347 Msa0553940:five_prime_UTR 45.0%
TGCTGTGCCAGAAAATGAGA+AGG + chr4_1:51224423-51224442 Msa0553940:five_prime_UTR 45.0%
TGGTTTATCGCTCTTTGCTG+CGG + chr4_1:51224708-51224727 Msa0553940:five_prime_UTR 45.0%
GCTCTACGGTCATTGTAATG+AGG + chr4_1:51224746-51224765 Msa0553940:five_prime_UTR 45.0%
TGTTGTTCCACCGATGTTGT+CGG + chr4_1:51224828-51224847 Msa0553940:five_prime_UTR 45.0%
!! GTTGTCGGCATTGACAATGA+AGG + chr4_1:51224843-51224862 Msa0553940:five_prime_UTR 45.0%
!! GGCATTGACAATGAAGGCAA+AGG + chr4_1:51224849-51224868 Msa0553940:five_prime_UTR 45.0%
! AGTTTGAGACAGGTCTCTTG+CGG + chr4_1:51224895-51224914 Msa0553940:five_prime_UTR 45.0%
AGAGGGCCTTCTATTATGAG+AGG + chr4_1:51225703-51225722 Msa0553940:five_prime_UTR 45.0%
! TGTGACTAACGTTCAGGTTC+CGG - chr4_1:51225803-51225822 None:intergenic 45.0%
!! AAAGGTTTGAGTTGCTGCAG+CGG - chr4_1:51225826-51225845 None:intergenic 45.0%
!!! CAAGGCCTTTTTTCACTAGG+TGG + chr4_1:51226078-51226097 Msa0553940:intron 45.0%
! AAGAAGGTACAGGTAGGCTA+AGG - chr4_1:51226174-51226193 None:intergenic 45.0%
AACACAGCTTCTAGATCAGC+AGG - chr4_1:51226526-51226545 None:intergenic 45.0%
!! CTTCATCTTCGGCACTGATA+TGG - chr4_1:51226712-51226731 None:intergenic 45.0%
!! GCATTTGTTGTCAAGAAGGC+TGG + chr4_1:51226752-51226771 Msa0553940:CDS 45.0%
TGTACTTTCTCCGAAGCATG+TGG + chr4_1:51226778-51226797 Msa0553940:CDS 45.0%
TTGCTCCATACAAGAAGGTC+CGG + chr4_1:51226937-51226956 Msa0553940:CDS 45.0%
TCTTCACCGACAAGATACCA+AGG + chr4_1:51226967-51226986 Msa0553940:CDS 45.0%
CGCCATGTCTTGTCTTTCTT+TGG - chr4_1:51227086-51227105 None:intergenic 45.0%
GTACAGGGCAGAGTAATTGA+TGG - chr4_1:51227298-51227317 None:intergenic 45.0%
CTTGTGCGGTTGTGCCTTAA+GGG - chr4_1:51223232-51223251 None:intergenic 50.0%
ACTTGTGCGGTTGTGCCTTA+AGG - chr4_1:51223233-51223252 None:intergenic 50.0%
AGGGCTAAAACCCCATCATG+AGG - chr4_1:51224091-51224110 None:intergenic 50.0%
GGGACTACAGGTGTGAGTAA+AGG + chr4_1:51224559-51224578 Msa0553940:five_prime_UTR 50.0%
GGTTTATCGCTCTTTGCTGC+GGG + chr4_1:51224709-51224728 Msa0553940:five_prime_UTR 50.0%
TGCTGCGGGATTATTGTCGT+TGG + chr4_1:51224723-51224742 Msa0553940:five_prime_UTR 50.0%
GCTGCGGGATTATTGTCGTT+GGG + chr4_1:51224724-51224743 Msa0553940:five_prime_UTR 50.0%
GACAACATCGGTGGAACAAC+AGG - chr4_1:51224829-51224848 None:intergenic 50.0%
AATGACTGAGTCGACTGCAG+TGG + chr4_1:51225543-51225562 Msa0553940:five_prime_UTR 50.0%
ATGACTGAGTCGACTGCAGT+GGG + chr4_1:51225544-51225563 Msa0553940:five_prime_UTR 50.0%
!! AGGGTTGTTGGCTCCAAACA+CGG + chr4_1:51225612-51225631 Msa0553940:five_prime_UTR 50.0%
TCCAAACACGGAGGCAAAAG+TGG + chr4_1:51225624-51225643 Msa0553940:five_prime_UTR 50.0%
!! ACCACTTTTGCCTCCGTGTT+TGG - chr4_1:51225628-51225647 None:intergenic 50.0%
!! ACGGCTGCATCGACTATTTC+AGG - chr4_1:51226559-51226578 None:intergenic 50.0%
GGTCGATTATGTTGCTGAGC+AGG + chr4_1:51226799-51226818 Msa0553940:CDS 50.0%
GCAGGTTGCTCCATACAAGA+AGG + chr4_1:51226932-51226951 Msa0553940:intron 50.0%
TCCATACAAGAAGGTCCGGA+AGG + chr4_1:51226941-51226960 Msa0553940:CDS 50.0%
ACCTTCCGGACCTTCTTGTA+TGG - chr4_1:51226945-51226964 None:intergenic 50.0%
TGTCGGTGAAGAACACCTTC+CGG - chr4_1:51226959-51226978 None:intergenic 50.0%
CGGAGACCTTGGTATCTTGT+CGG - chr4_1:51226976-51226995 None:intergenic 50.0%
AAGATACCAAGGTCTCCGAC+TGG + chr4_1:51226978-51226997 Msa0553940:CDS 50.0%
AGATACCAAGGTCTCCGACT+GGG + chr4_1:51226979-51226998 Msa0553940:CDS 50.0%
TTTCGGAGGATCTTCCCAGT+CGG - chr4_1:51226996-51227015 None:intergenic 50.0%
AGATCCTCCGAAAGCAACTC+AGG + chr4_1:51227003-51227022 Msa0553940:CDS 50.0%
! AGCAGTGCCTGAGTTGCTTT+CGG - chr4_1:51227013-51227032 None:intergenic 50.0%
TACTCTGCCCTGTACACCTT+TGG + chr4_1:51227303-51227322 Msa0553940:three_prime_UTR 50.0%
AGGCTGTGGCTTGAGTGTGA+AGG - chr4_1:51223785-51223804 None:intergenic 55.0%
CTCAACACCCTCACCCTCAT+TGG + chr4_1:51223969-51223988 Msa0553940:five_prime_UTR 55.0%
GTCAATGCCGACAACATCGG+TGG - chr4_1:51224838-51224857 None:intergenic 55.0%
GGTGCAGCACCTTTGAGCAT+AGG + chr4_1:51224916-51224935 Msa0553940:five_prime_UTR 55.0%
!! GTTGTTGGCTCCAAACACGG+AGG + chr4_1:51225615-51225634 Msa0553940:five_prime_UTR 55.0%
CGGAGGCAAAAGTGGTAGAC+TGG + chr4_1:51225632-51225651 Msa0553940:five_prime_UTR 55.0%
! TTTTTACCCCCAGGCAGCTG+CGG + chr4_1:51225667-51225686 Msa0553940:five_prime_UTR 55.0%
! TGCGGTGAGCTTTGGTTGAG+AGG + chr4_1:51225685-51225704 Msa0553940:five_prime_UTR 55.0%
! GCGGTGAGCTTTGGTTGAGA+GGG + chr4_1:51225686-51225705 Msa0553940:five_prime_UTR 55.0%
CAGCGGTGTGACTAACGTTC+AGG - chr4_1:51225809-51225828 None:intergenic 55.0%
ATCTTCCCAGTCGGAGACCT+TGG - chr4_1:51226987-51227006 None:intergenic 55.0%
! AGTGCCTGAGTTGCTTTCGG+AGG - chr4_1:51227010-51227029 None:intergenic 55.0%
TCGACTGCAGTGGGAACACG+TGG + chr4_1:51225553-51225572 Msa0553940:five_prime_UTR 60.0%
AAAGCTCACCGCAGCTGCCT+GGG - chr4_1:51225678-51225697 None:intergenic 60.0%
AAGCTCACCGCAGCTGCCTG+GGG - chr4_1:51225677-51225696 None:intergenic 65.0%
CAAAGCTCACCGCAGCTGCC+TGG - chr4_1:51225679-51225698 None:intergenic 65.0%
CAGGCAGCTGCGGTGAGCTT+TGG + chr4_1:51225677-51225696 Msa0553940:five_prime_UTR 65.0%
AGCTCACCGCAGCTGCCTGG+GGG - chr4_1:51225676-51225695 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 51223060 51228033 51223060 ID=Msa0553940;Name=Msa0553940
chr4_1 mRNA 51223060 51228033 51223060 ID=Msa0553940-mRNA-1;Parent=Msa0553940;Name=Msa0553940-mRNA-1;_AED=0.03;_eAED=0.03;_QI=2316|1|1|1|1|0.8|5|984|121
chr4_1 exon 51223060 51224987 51223060 ID=Msa0553940-mRNA-1:exon:14601;Parent=Msa0553940-mRNA-1
chr4_1 exon 51225535 51225724 51225535 ID=Msa0553940-mRNA-1:exon:14602;Parent=Msa0553940-mRNA-1
chr4_1 exon 51226260 51226405 51226260 ID=Msa0553940-mRNA-1:exon:14603;Parent=Msa0553940-mRNA-1
chr4_1 exon 51226517 51226820 51226517 ID=Msa0553940-mRNA-1:exon:14604;Parent=Msa0553940-mRNA-1
chr4_1 exon 51226936 51228033 51226936 ID=Msa0553940-mRNA-1:exon:14605;Parent=Msa0553940-mRNA-1
chr4_1 five_prime_UTR 51223060 51224987 51223060 ID=Msa0553940-mRNA-1:five_prime_utr;Parent=Msa0553940-mRNA-1
chr4_1 five_prime_UTR 51225535 51225724 51225535 ID=Msa0553940-mRNA-1:five_prime_utr;Parent=Msa0553940-mRNA-1
chr4_1 five_prime_UTR 51226260 51226405 51226260 ID=Msa0553940-mRNA-1:five_prime_utr;Parent=Msa0553940-mRNA-1
chr4_1 five_prime_UTR 51226517 51226568 51226517 ID=Msa0553940-mRNA-1:five_prime_utr;Parent=Msa0553940-mRNA-1
chr4_1 CDS 51226569 51226820 51226569 ID=Msa0553940-mRNA-1:cds;Parent=Msa0553940-mRNA-1
chr4_1 CDS 51226936 51227049 51226936 ID=Msa0553940-mRNA-1:cds;Parent=Msa0553940-mRNA-1
chr4_1 three_prime_UTR 51227050 51228033 51227050 ID=Msa0553940-mRNA-1:three_prime_utr;Parent=Msa0553940-mRNA-1
Gene Sequence

>Msa0553940

ATGCAGCCGTTACAGCGTAAGTATATGAACACTTCATTTATGTCTTCGCTTCTGCAATCTATAGAAAATGCAGCCATCCGTAATCTTCTAAAGTGCCTCTTTGGACATTTGATTATAACCTCAAATGTTACGTCATTTTTCCATATCAGTGCCGAAGATGAAGAAATTGGGGAAATTCCAGTGGCATTTGTTGTCAAGAAGGCTGGAAGTGTACTTTCTCCGAAGCATGTGGTCGATTATGTTGCTGAGCAGGTTGCTCCATACAAGAAGGTCCGGAAGGTGTTCTTCACCGACAAGATACCAAGGTCTCCGACTGGGAAGATCCTCCGAAAGCAACTCAGGCACTGCTTGAGTTCTAAACTCTAA
Protein sequence

>Msa0553940

MQPLQRKYMNTSFMSSLLQSIENAAIRNLLKCLFGHLIITSNVTSFFHISAEDEEIGEIPVAFVVKKAGSVLSPKHVVDYVAEQVAPYKKVRKVFFTDKIPRSPTGKILRKQLRHCLSSKL*