AlfalfaGEDB | Msa0649130

M. sativa cultivar zhongmu-4 / Msa0649130

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0649130	XP_024638655.1	96.875	32	1	0	1	32	1	32	3.90e-13	73.2

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0649130	G7JCJ5	96.875	32	1	0	1	32	1	32	1.86e-13	73.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0004840	Msa0649130	0.800168	1.635428e-48	-8.615850e-47
Msa0014800	Msa0649130	0.826274	2.967544e-54	-8.615850e-47
Msa0037880	Msa0649130	0.826830	2.187362e-54	-8.615850e-47
Msa0043980	Msa0649130	0.817557	3.099601e-52	-8.615850e-47
Msa0049750	Msa0649130	0.801275	9.724233e-49	-8.615850e-47
Msa0052030	Msa0649130	0.820177	7.870375e-53	-8.615850e-47
Msa0084430	Msa0649130	0.804438	2.160117e-49	-8.615850e-47
Msa0089360	Msa0649130	0.812090	5.048899e-51	-8.615850e-47
Msa0096450	Msa0649130	0.801976	6.984507e-49	-8.615850e-47
Msa0096980	Msa0649130	0.814142	1.791059e-51	-8.615850e-47
Msa0104800	Msa0649130	0.807839	4.154501e-50	-8.615850e-47
Msa0105700	Msa0649130	0.814786	1.290422e-51	-8.615850e-47
Msa0108560	Msa0649130	0.824177	9.298520e-54	-8.615850e-47
Msa0117770	Msa0649130	0.805201	1.496561e-49	-8.615850e-47
Msa0120340	Msa0649130	0.836031	1.187191e-56	-8.615850e-47
Msa0608710	Msa0649130	0.827767	1.304492e-54	-8.615850e-47
Msa0615340	Msa0649130	0.806264	8.952103e-50	-8.615850e-47
Msa0626990	Msa0649130	0.820667	6.075776e-53	-8.615850e-47
Msa0627350	Msa0649130	0.825054	5.777629e-54	-8.615850e-47
Msa0628740	Msa0649130	0.811530	6.685685e-51	-8.615850e-47
Msa0633060	Msa0649130	0.808853	2.525474e-50	-8.615850e-47
Msa0637070	Msa0649130	0.802958	4.382162e-49	-8.615850e-47
Msa0649130	Msa0653490	0.808187	3.502425e-50	-8.615850e-47
Msa0649130	Msa0660300	0.821125	4.767300e-53	-8.615850e-47
Msa0649130	Msa0666140	0.806191	9.271549e-50	-8.615850e-47
Msa0649130	Msa0679550	0.814966	1.177111e-51	-8.615850e-47
Msa0649130	Msa0688070	0.821712	3.489043e-53	-8.615850e-47
Msa0649130	Msa0698360	0.805280	1.440961e-49	-8.615850e-47
Msa0649130	Msa0731180	0.832113	1.137399e-55	-8.615850e-47
Msa0649130	Msa0767220	0.806806	6.877777e-50	-8.615850e-47
Msa0649130	Msa0771580	0.814247	1.697721e-51	-8.615850e-47
Msa0649130	Msa0771720	0.829416	5.212550e-55	-8.615850e-47
Msa0649130	Msa0794320	0.811938	5.450928e-51	-8.615850e-47
Msa0649130	Msa0795080	0.814220	1.721072e-51	-8.615850e-47
Msa0649130	Msa0801740	0.800558	1.362490e-48	-8.615850e-47
Msa0649130	Msa0835730	0.803022	4.251426e-49	-8.615850e-47
Msa0649130	Msa0869120	0.819510	1.118566e-52	-8.615850e-47
Msa0649130	Msa0891600	-0.802104	6.572293e-49	-8.615850e-47
Msa0649130	Msa0899230	0.810889	9.208038e-51	-8.615850e-47
Msa0649130	Msa0910980	-0.829319	5.500649e-55	-8.615850e-47
Msa0649130	Msa0927670	0.811820	5.781056e-51	-8.615850e-47
Msa0649130	Msa0944550	0.824474	7.917082e-54	-8.615850e-47
Msa0649130	Msa0958920	0.820572	6.390431e-53	-8.615850e-47
Msa0649130	Msa0960160	0.806135	9.528346e-50	-8.615850e-47
Msa0649130	Msa0982270	0.843597	1.270895e-58	-8.615850e-47
Msa0649130	Msa0992490	-0.803711	3.060062e-49	-8.615850e-47
Msa0649130	Msa1002480	0.803754	2.997071e-49	-8.615850e-47
Msa0649130	Msa1023920	0.805129	1.549659e-49	-8.615850e-47
Msa0649130	Msa1032440	0.824828	6.533358e-54	-8.615850e-47
Msa0649130	Msa1034150	0.801745	7.789474e-49	-8.615850e-47
Msa0649130	Msa1082500	0.805181	1.511184e-49	-8.615850e-47
Msa0649130	Msa1090260	0.805278	1.442275e-49	-8.615850e-47
Msa0649130	Msa1092090	0.834816	2.408325e-56	-8.615850e-47
Msa0649130	Msa1118110	0.820267	7.507477e-53	-8.615850e-47
Msa0649130	Msa1151710	0.805023	1.630523e-49	-8.615850e-47
Msa0649130	Msa1174310	0.804364	2.238580e-49	-8.615850e-47
Msa0649130	Msa1206100	0.803116	4.064321e-49	-8.615850e-47
Msa0649130	Msa1215920	0.800998	1.108054e-48	-8.615850e-47
Msa0649130	Msa1224480	0.821086	4.867133e-53	-8.615850e-47
Msa0649130	Msa1230830	0.804970	1.672831e-49	-8.615850e-47
Msa0649130	Msa1233500	0.838091	3.533994e-57	-8.615850e-47
Msa0649130	Msa1237980	0.811327	7.398999e-51	-8.615850e-47
Msa0649130	Msa1267520	0.804812	1.804573e-49	-8.615850e-47
Msa0649130	Msa1270660	0.802433	5.622146e-49	-8.615850e-47
Msa0649130	Msa1270730	0.819034	1.435636e-52	-8.615850e-47
Msa0649130	Msa1276440	0.843051	1.776906e-58	-8.615850e-47
Msa0649130	Msa1279210	0.812805	3.524199e-51	-8.615850e-47
Msa0649130	Msa1296940	0.816261	6.058266e-52	-8.615850e-47
Msa0649130	Msa1306750	0.839317	1.703390e-57	-8.615850e-47
Msa0649130	Msa1307740	0.839845	1.241512e-57	-8.615850e-47
Msa0649130	Msa1314340	0.807559	4.765069e-50	-8.615850e-47
Msa0649130	Msa1314770	0.809202	2.126239e-50	-8.615850e-47
Msa0649130	Msa1322190	0.814375	1.590914e-51	-8.615850e-47
Msa0649130	Msa1329550	0.801260	9.793788e-49	-8.615850e-47
Msa0649130	Msa1333730	0.807469	4.978716e-50	-8.615850e-47
Msa0649130	Msa1348600	0.804938	1.698396e-49	-8.615850e-47
Msa0649130	Msa1407600	0.849671	2.784099e-60	-8.615850e-47
Msa0649130	Msa1444250	-0.804103	2.536791e-49	-8.615850e-47
Msa0649130	Msa1444740	0.846406	2.216090e-59	-8.615850e-47
Msa0649130	Msa1451620	-0.820058	8.383186e-53	-8.615850e-47
Msa0649130	Msa1462060	0.808595	2.867728e-50	-8.615850e-47
Msa0649130	Msa1466730	0.811476	6.869508e-51	-8.615850e-47
Msa0122590	Msa0649130	0.831234	1.873977e-55	-8.615850e-47
Msa0137570	Msa0649130	0.814668	1.370540e-51	-8.615850e-47
Msa0145010	Msa0649130	0.806436	8.233989e-50	-8.615850e-47
Msa0145310	Msa0649130	0.811280	7.574708e-51	-8.615850e-47
Msa0152170	Msa0649130	0.821966	3.048446e-53	-8.615850e-47
Msa0153830	Msa0649130	0.800296	1.540632e-48	-8.615850e-47
Msa0157100	Msa0649130	0.821833	3.271152e-53	-8.615850e-47
Msa0159770	Msa0649130	0.818344	2.058906e-52	-8.615850e-47
Msa0182570	Msa0649130	0.805173	1.517064e-49	-8.615850e-47
Msa0187220	Msa0649130	0.805270	1.447626e-49	-8.615850e-47
Msa0205510	Msa0649130	0.800932	1.142612e-48	-8.615850e-47
Msa0206010	Msa0649130	0.820369	7.113627e-53	-8.615850e-47
Msa0220440	Msa0649130	0.820475	6.724610e-53	-8.615850e-47
Msa0365490	Msa0649130	0.802922	4.457179e-49	-8.615850e-47
Msa0379640	Msa0649130	0.816443	5.514948e-52	-8.615850e-47
Msa0388840	Msa0649130	0.817222	3.687929e-52	-8.615850e-47
Msa0389810	Msa0649130	0.844598	6.847680e-59	-8.615850e-47
Msa0394340	Msa0649130	0.813962	1.962651e-51	-8.615850e-47
Msa0409200	Msa0649130	0.800330	1.516362e-48	-8.615850e-47
Msa0431690	Msa0649130	0.817014	4.107478e-52	-8.615850e-47
Msa0433360	Msa0649130	0.805282	1.438949e-49	-8.615850e-47
Msa0433410	Msa0649130	-0.821683	3.543309e-53	-8.615850e-47
Msa0433530	Msa0649130	0.811141	8.119385e-51	-8.615850e-47
Msa0442200	Msa0649130	0.801321	9.512828e-49	-8.615850e-47
Msa0444090	Msa0649130	0.803133	4.032098e-49	-8.615850e-47
Msa0458440	Msa0649130	0.813432	2.567798e-51	-8.615850e-47
Msa0468130	Msa0649130	0.836560	8.710352e-57	-8.615850e-47
Msa0472960	Msa0649130	-0.801585	8.401208e-49	-8.615850e-47
Msa0477130	Msa0649130	-0.819987	8.700800e-53	-8.615850e-47
Msa0477260	Msa0649130	0.806244	9.037893e-50	-8.615850e-47
Msa0482190	Msa0649130	-0.800757	1.240850e-48	-8.615850e-47
Msa0249100	Msa0649130	0.821315	4.310613e-53	-8.615850e-47
Msa0287410	Msa0649130	0.812747	3.628244e-51	-8.615850e-47
Msa0299580	Msa0649130	0.813112	3.017971e-51	-8.615850e-47
Msa0309550	Msa0649130	0.811092	8.322296e-51	-8.615850e-47
Msa0315290	Msa0649130	0.810155	1.326894e-50	-8.615850e-47
Msa0319170	Msa0649130	0.821555	3.793458e-53	-8.615850e-47
Msa0326110	Msa0649130	0.820416	6.940583e-53	-8.615850e-47
Msa0337060	Msa0649130	0.813294	2.753476e-51	-8.615850e-47
Msa0344910	Msa0649130	0.807846	4.139773e-50	-8.615850e-47
Msa0344980	Msa0649130	0.800449	1.433801e-48	-8.615850e-47
Msa0484780	Msa0649130	0.812542	4.024197e-51	-8.615850e-47
Msa0489610	Msa0649130	0.804286	2.324066e-49	-8.615850e-47
Msa0530650	Msa0649130	0.826469	2.667338e-54	-8.615850e-47
Msa0575470	Msa0649130	0.816199	6.255035e-52	-8.615850e-47
Msa0587190	Msa0649130	0.822822	1.928639e-53	-8.615850e-47
Msa0588240	Msa0649130	0.801200	1.007479e-48	-8.615850e-47
Msa0594790	Msa0649130	0.816450	5.497065e-52	-8.615850e-47
Msa0599580	Msa0649130	0.815353	9.655097e-52	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0649130	MtrunA17_Chr4g0041021	96.875	32	1	0	1	32	1	32	3.66e-17	73.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 40 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTATGCCTCAATTCTTTAAA+AGG	0.159091	4_3:+55720735	None:intergenic
CTTTCTCTTTCTTTATCTTT+TGG	0.191821	4_3:-55720759	Msa0649130:five_prime_UTR
TGGATGTGGACGGCATAAAT+TGG	0.210693	4_3:-55719988	Msa0649130:CDS
CGCCTCTAGCTGGTTTAAAC+AGG	0.301623	4_3:-55719616	Msa0649130:CDS
CTTATAAGTTGAGACAATTC+AGG	0.344668	4_3:-55720048	Msa0649130:five_prime_UTR
AATGCTTACAACAATAATTA+AGG	0.350956	4_3:+55719677	None:intergenic
ATCATCAACCCTTCTTCGTT+CGG	0.364084	4_3:-55720713	Msa0649130:five_prime_UTR
CCCTTCTTCGTTCGGTTCAA+AGG	0.379770	4_3:-55720705	Msa0649130:five_prime_UTR
AGAGTTGTGACTTGAGAAGA+AGG	0.409768	4_3:+55720819	None:intergenic
TATGCCTCAATTCTTTAAAA+GGG	0.439571	4_3:+55720736	None:intergenic
CCTTCTTCGTTCGGTTCAAA+GGG	0.457644	4_3:-55720704	Msa0649130:intron
AGAAGAAGGGTACGAGGAAG+AGG	0.463956	4_3:+55720833	None:intergenic
CCCTTTGAACCGAACGAAGA+AGG	0.465518	4_3:+55720704	None:intergenic
CAAGGAAACGTGCACCTTGA+AGG	0.469065	4_3:+55720869	None:intergenic
GACAATTTACGATTGTTTGA+AGG	0.474352	4_3:-55720026	Msa0649130:five_prime_UTR
GCTAGAGGCGCTGAGACCTT+CGG	0.481815	4_3:+55719629	None:intergenic
AGCAGAAGCTAAAACCTTCA+AGG	0.486400	4_3:-55720883	Msa0649130:five_prime_UTR
GCTTCTGCTATTCTGGCGCT+AGG	0.486613	4_3:+55720897	None:intergenic
ATATCATGTCTTCTGCCTCC+AGG	0.497633	4_3:+55719930	None:intergenic
AAGGTCTCAGCGCCTCTAGC+TGG	0.498136	4_3:-55719626	Msa0649130:CDS
GAAATTTGAAAATATGTGCC+TGG	0.506581	4_3:-55719948	Msa0649130:CDS
TATTATGATCACCATGGATG+TGG	0.516106	4_3:-55720002	Msa0649130:exon
CATGTCTAATACCTCGCTAA+AGG	0.537069	4_3:+55719591	None:intergenic
CCTTTGAACCGAACGAAGAA+GGG	0.538606	4_3:+55720705	None:intergenic
ATTTATGCCGTCCACATCCA+TGG	0.554553	4_3:+55719991	None:intergenic
GGATGTGGACGGCATAAATT+GGG	0.555198	4_3:-55719987	Msa0649130:CDS
GTGGACGGCATAAATTGGGT+TGG	0.566544	4_3:-55719983	Msa0649130:CDS
ACCTTCGGATGCTTGCCACA+TGG	0.573875	4_3:+55719644	None:intergenic
GAGTTGTGACTTGAGAAGAA+GGG	0.625930	4_3:+55720820	None:intergenic
TTAAACAGGGTCCTTTAGCG+AGG	0.626587	4_3:-55719602	Msa0649130:CDS
GCCATGTGGCAAGCATCCGA+AGG	0.633407	4_3:-55719645	Msa0649130:CDS
ATGATCACCATGGATGTGGA+CGG	0.637739	4_3:-55719998	Msa0649130:CDS
GCCTCTAGCTGGTTTAAACA+GGG	0.652544	4_3:-55719615	Msa0649130:CDS
GAAGGATATTATGATCACCA+TGG	0.654134	4_3:-55720008	Msa0649130:exon
GACTTGAGAAGAAGGGTACG+AGG	0.672383	4_3:+55720827	None:intergenic
ACCCTGTTTAAACCAGCTAG+AGG	0.677442	4_3:+55719614	None:intergenic
TGAAATGAAGAGTACACGTG+TGG	0.699903	4_3:-55720924	Msa0649130:five_prime_UTR
ATTTGAAAATATGTGCCTGG+AGG	0.710944	4_3:-55719945	Msa0649130:CDS
GGCAGAAGACATGATATACG+AGG	0.734207	4_3:-55719924	Msa0649130:intron
GCATTGTAGTCAAAGCCATG+TGG	0.735505	4_3:-55719659	Msa0649130:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TAAAATGAAATAGATAAATA+AGG	+	chr4_3:55719943-55719962	None:intergenic	10.0%
!!	TAAAATGAAATAGATAAATA+AGG	+	chr4_3:55719943-55719962	None:intergenic	10.0%
!!!	GTTTTAGAAGATAAAGTTAT+TGG	-	chr4_3:55720167-55720186	Msa0649130:intron	20.0%
!!!	GGAAATTATGGTTTTAATTT+TGG	-	chr4_3:55720227-55720246	Msa0649130:intron	20.0%
!!	TAGTTAATTACCTGATTAAA+TGG	-	chr4_3:55720256-55720275	Msa0649130:intron	20.0%
!!	TAATAACAAACCATTTAATC+AGG	+	chr4_3:55720269-55720288	None:intergenic	20.0%
!!!	TTATTATTGCTTCATGTTTT+TGG	-	chr4_3:55720283-55720302	Msa0649130:intron	20.0%
!!!	AATTGCAAAACACAATTTTA+GGG	+	chr4_3:55720807-55720826	None:intergenic	20.0%
!!	AATGCTTACAACAATAATTA+AGG	+	chr4_3:55720887-55720906	None:intergenic	20.0%
!!!	GTTTTAGAAGATAAAGTTAT+TGG	-	chr4_3:55720167-55720186	Msa0649130:intron	20.0%
!!!	GGAAATTATGGTTTTAATTT+TGG	-	chr4_3:55720227-55720246	Msa0649130:intron	20.0%
!!	TAGTTAATTACCTGATTAAA+TGG	-	chr4_3:55720256-55720275	Msa0649130:intron	20.0%
!!	TAATAACAAACCATTTAATC+AGG	+	chr4_3:55720269-55720288	None:intergenic	20.0%
!!!	TTATTATTGCTTCATGTTTT+TGG	-	chr4_3:55720283-55720302	Msa0649130:intron	20.0%
!!!	AATTGCAAAACACAATTTTA+GGG	+	chr4_3:55720807-55720826	None:intergenic	20.0%
!!	AATGCTTACAACAATAATTA+AGG	+	chr4_3:55720887-55720906	None:intergenic	20.0%
!!	CTTTCTCTTTCTTTATCTTT+TGG	-	chr4_3:55719802-55719821	Msa0649130:intron	25.0%
!!	TATGCCTCAATTCTTTAAAA+GGG	+	chr4_3:55719828-55719847	None:intergenic	25.0%
!	TTATGCCTCAATTCTTTAAA+AGG	+	chr4_3:55719829-55719848	None:intergenic	25.0%
!	ATGTTTGGTAAATTAGTTGA+AGG	-	chr4_3:55720134-55720153	Msa0649130:intron	25.0%
!	TTATTGGATTGTTCTGTTAT+TGG	-	chr4_3:55720183-55720202	Msa0649130:intron	25.0%
!	TGTTATGTCTTTATGATTAG+TGG	-	chr4_3:55720374-55720393	Msa0649130:intron	25.0%
!!	TTCATGATCAATGTACTTTT+AGG	-	chr4_3:55720403-55720422	Msa0649130:intron	25.0%
!	CTCATATTGTATATACACAA+TGG	-	chr4_3:55720692-55720711	Msa0649130:intron	25.0%
!!	TGGATTCTTTAGTTTCAAAT+CGG	-	chr4_3:55720712-55720731	Msa0649130:five_prime_UTR	25.0%
!!	GAATTGCAAAACACAATTTT+AGG	+	chr4_3:55720808-55720827	None:intergenic	25.0%
!	AATAATTAAGGCAGAATACT+AGG	+	chr4_3:55720875-55720894	None:intergenic	25.0%
!!	CTTTCTCTTTCTTTATCTTT+TGG	-	chr4_3:55719802-55719821	Msa0649130:intron	25.0%
!!	TATGCCTCAATTCTTTAAAA+GGG	+	chr4_3:55719828-55719847	None:intergenic	25.0%
!	TTATGCCTCAATTCTTTAAA+AGG	+	chr4_3:55719829-55719848	None:intergenic	25.0%
!	ATGTTTGGTAAATTAGTTGA+AGG	-	chr4_3:55720134-55720153	Msa0649130:intron	25.0%
!	TTATTGGATTGTTCTGTTAT+TGG	-	chr4_3:55720183-55720202	Msa0649130:intron	25.0%
!	TGTTATGTCTTTATGATTAG+TGG	-	chr4_3:55720374-55720393	Msa0649130:intron	25.0%
!!	TTCATGATCAATGTACTTTT+AGG	-	chr4_3:55720403-55720422	Msa0649130:intron	25.0%
!	CTCATATTGTATATACACAA+TGG	-	chr4_3:55720692-55720711	Msa0649130:intron	25.0%
!!	TGGATTCTTTAGTTTCAAAT+CGG	-	chr4_3:55720712-55720731	Msa0649130:five_prime_UTR	25.0%
!!	GAATTGCAAAACACAATTTT+AGG	+	chr4_3:55720808-55720827	None:intergenic	25.0%
!	AATAATTAAGGCAGAATACT+AGG	+	chr4_3:55720875-55720894	None:intergenic	25.0%
	AGAGGAACAAAAACAAAACA+AGG	+	chr4_3:55719713-55719732	None:intergenic	30.0%
	CATAAAACAAAAACCCATGA+AGG	+	chr4_3:55719890-55719909	None:intergenic	30.0%
	ACAAAGAAGTTAAACCTTGT+AGG	-	chr4_3:55720109-55720128	Msa0649130:intron	30.0%
	TAAACCTTGTAGGTTATGTT+TGG	-	chr4_3:55720119-55720138	Msa0649130:intron	30.0%
	TTTACCAAACATAACCTACA+AGG	+	chr4_3:55720126-55720145	None:intergenic	30.0%
!	GGTAAATTAGTTGAAGGTTT+TGG	-	chr4_3:55720140-55720159	Msa0649130:intron	30.0%
!!	ATTAGTTGAAGGTTTTGGTA+TGG	-	chr4_3:55720145-55720164	Msa0649130:intron	30.0%
!	CTTATAAGTTGAGACAATTC+AGG	-	chr4_3:55720513-55720532	Msa0649130:intron	30.0%
	GACAATTTACGATTGTTTGA+AGG	-	chr4_3:55720535-55720554	Msa0649130:intron	30.0%
	GAAATTTGAAAATATGTGCC+TGG	-	chr4_3:55720613-55720632	Msa0649130:intron	30.0%
	AGAGGAACAAAAACAAAACA+AGG	+	chr4_3:55719713-55719732	None:intergenic	30.0%
	CATAAAACAAAAACCCATGA+AGG	+	chr4_3:55719890-55719909	None:intergenic	30.0%
	ACAAAGAAGTTAAACCTTGT+AGG	-	chr4_3:55720109-55720128	Msa0649130:intron	30.0%
	TAAACCTTGTAGGTTATGTT+TGG	-	chr4_3:55720119-55720138	Msa0649130:intron	30.0%
	TTTACCAAACATAACCTACA+AGG	+	chr4_3:55720126-55720145	None:intergenic	30.0%
!	GGTAAATTAGTTGAAGGTTT+TGG	-	chr4_3:55720140-55720159	Msa0649130:intron	30.0%
!!	ATTAGTTGAAGGTTTTGGTA+TGG	-	chr4_3:55720145-55720164	Msa0649130:intron	30.0%
!	CTTATAAGTTGAGACAATTC+AGG	-	chr4_3:55720513-55720532	Msa0649130:intron	30.0%
	GACAATTTACGATTGTTTGA+AGG	-	chr4_3:55720535-55720554	Msa0649130:intron	30.0%
	GAAATTTGAAAATATGTGCC+TGG	-	chr4_3:55720613-55720632	Msa0649130:intron	30.0%
!!	TTGGCCCTTTTAAAGAATTG+AGG	-	chr4_3:55719821-55719840	Msa0649130:intron	35.0%
!!	AAAGGGTATTTCTCCTTCAT+GGG	-	chr4_3:55719874-55719893	Msa0649130:intron	35.0%
!!	GTTGAAGCTTTTGACTGTTT+AGG	-	chr4_3:55720086-55720105	Msa0649130:intron	35.0%
	TGTTATTGGCAGGTACAAAA+TGG	-	chr4_3:55720197-55720216	Msa0649130:intron	35.0%
	GTTATTGGCAGGTACAAAAT+GGG	-	chr4_3:55720198-55720217	Msa0649130:intron	35.0%
	AATCTATTTGTGTCATGAGC+TGG	-	chr4_3:55720328-55720347	Msa0649130:intron	35.0%
	GAAGGATATTATGATCACCA+TGG	-	chr4_3:55720553-55720572	Msa0649130:intron	35.0%
	TATTATGATCACCATGGATG+TGG	-	chr4_3:55720559-55720578	Msa0649130:intron	35.0%
	ATTTGAAAATATGTGCCTGG+AGG	-	chr4_3:55720616-55720635	Msa0649130:intron	35.0%
	ATGACATGTATCACAAGCAA+TGG	+	chr4_3:55720769-55720788	None:intergenic	35.0%
!!	TTGGCCCTTTTAAAGAATTG+AGG	-	chr4_3:55719821-55719840	Msa0649130:intron	35.0%
!!	AAAGGGTATTTCTCCTTCAT+GGG	-	chr4_3:55719874-55719893	Msa0649130:intron	35.0%
!!	GTTGAAGCTTTTGACTGTTT+AGG	-	chr4_3:55720086-55720105	Msa0649130:intron	35.0%
	TGTTATTGGCAGGTACAAAA+TGG	-	chr4_3:55720197-55720216	Msa0649130:intron	35.0%
	GTTATTGGCAGGTACAAAAT+GGG	-	chr4_3:55720198-55720217	Msa0649130:intron	35.0%
	AATCTATTTGTGTCATGAGC+TGG	-	chr4_3:55720328-55720347	Msa0649130:intron	35.0%
	GAAGGATATTATGATCACCA+TGG	-	chr4_3:55720553-55720572	Msa0649130:intron	35.0%
	TATTATGATCACCATGGATG+TGG	-	chr4_3:55720559-55720578	Msa0649130:intron	35.0%
	ATTTGAAAATATGTGCCTGG+AGG	-	chr4_3:55720616-55720635	Msa0649130:intron	35.0%
	ATGACATGTATCACAAGCAA+TGG	+	chr4_3:55720769-55720788	None:intergenic	35.0%
!	TGAAATGAAGAGTACACGTG+TGG	-	chr4_3:55719637-55719656	Msa0649130:CDS	40.0%
!!!	GGTTTTAGCTTCTGCTATTC+TGG	+	chr4_3:55719674-55719693	None:intergenic	40.0%
	AGCAGAAGCTAAAACCTTCA+AGG	-	chr4_3:55719678-55719697	Msa0649130:CDS	40.0%
	GAGTTGTGACTTGAGAAGAA+GGG	+	chr4_3:55719744-55719763	None:intergenic	40.0%
	AGAGTTGTGACTTGAGAAGA+AGG	+	chr4_3:55719745-55719764	None:intergenic	40.0%
	ATCATCAACCCTTCTTCGTT+CGG	-	chr4_3:55719848-55719867	Msa0649130:intron	40.0%
!!	CAAAGGGTATTTCTCCTTCA+TGG	-	chr4_3:55719873-55719892	Msa0649130:intron	40.0%
	TGAGACAGAAGATTCTGTTG+AGG	-	chr4_3:55720063-55720082	Msa0649130:five_prime_UTR	40.0%
	GAGACAGAAGATTCTGTTGA+GGG	-	chr4_3:55720064-55720083	Msa0649130:five_prime_UTR	40.0%
	TGGATTGTTCTGTTATTGGC+AGG	-	chr4_3:55720187-55720206	Msa0649130:intron	40.0%
	AATGGGACAGTGGGAAATTA+TGG	-	chr4_3:55720215-55720234	Msa0649130:intron	40.0%
	TTTGTGTCATGAGCTGGAAA+TGG	-	chr4_3:55720334-55720353	Msa0649130:intron	40.0%
	CATGTATCACAAGCAATGGT+AGG	+	chr4_3:55720765-55720784	None:intergenic	40.0%
	CTAGGTACACATAAACCAAG+AGG	+	chr4_3:55720857-55720876	None:intergenic	40.0%
!	TGAAATGAAGAGTACACGTG+TGG	-	chr4_3:55719637-55719656	Msa0649130:CDS	40.0%
!!!	GGTTTTAGCTTCTGCTATTC+TGG	+	chr4_3:55719674-55719693	None:intergenic	40.0%
	AGCAGAAGCTAAAACCTTCA+AGG	-	chr4_3:55719678-55719697	Msa0649130:CDS	40.0%
	GAGTTGTGACTTGAGAAGAA+GGG	+	chr4_3:55719744-55719763	None:intergenic	40.0%
	AGAGTTGTGACTTGAGAAGA+AGG	+	chr4_3:55719745-55719764	None:intergenic	40.0%
	ATCATCAACCCTTCTTCGTT+CGG	-	chr4_3:55719848-55719867	Msa0649130:intron	40.0%
!!	CAAAGGGTATTTCTCCTTCA+TGG	-	chr4_3:55719873-55719892	Msa0649130:intron	40.0%
	TGAGACAGAAGATTCTGTTG+AGG	-	chr4_3:55720063-55720082	Msa0649130:five_prime_UTR	40.0%
	GAGACAGAAGATTCTGTTGA+GGG	-	chr4_3:55720064-55720083	Msa0649130:five_prime_UTR	40.0%
	TGGATTGTTCTGTTATTGGC+AGG	-	chr4_3:55720187-55720206	Msa0649130:intron	40.0%
	AATGGGACAGTGGGAAATTA+TGG	-	chr4_3:55720215-55720234	Msa0649130:intron	40.0%
	TTTGTGTCATGAGCTGGAAA+TGG	-	chr4_3:55720334-55720353	Msa0649130:intron	40.0%
	CATGTATCACAAGCAATGGT+AGG	+	chr4_3:55720765-55720784	None:intergenic	40.0%
	CTAGGTACACATAAACCAAG+AGG	+	chr4_3:55720857-55720876	None:intergenic	40.0%
	CCTTTGAACCGAACGAAGAA+GGG	+	chr4_3:55719859-55719878	None:intergenic	45.0%
	CCTTCTTCGTTCGGTTCAAA+GGG	-	chr4_3:55719857-55719876	Msa0649130:intron	45.0%
	CAGGTACAAAATGGGACAGT+GGG	-	chr4_3:55720206-55720225	Msa0649130:intron	45.0%
	ATGATCACCATGGATGTGGA+CGG	-	chr4_3:55720563-55720582	Msa0649130:intron	45.0%
	ATTTATGCCGTCCACATCCA+TGG	+	chr4_3:55720573-55720592	None:intergenic	45.0%
	TGGATGTGGACGGCATAAAT+TGG	-	chr4_3:55720573-55720592	Msa0649130:intron	45.0%
	GGATGTGGACGGCATAAATT+GGG	-	chr4_3:55720574-55720593	Msa0649130:intron	45.0%
	ATATCATGTCTTCTGCCTCC+AGG	+	chr4_3:55720634-55720653	None:intergenic	45.0%
	GGCAGAAGACATGATATACG+AGG	-	chr4_3:55720637-55720656	Msa0649130:intron	45.0%
	AGGAAATGCAGCTCTACATG+CGG	+	chr4_3:55720837-55720856	None:intergenic	45.0%
	TGTAGAGCTGCATTTCCTCT+TGG	-	chr4_3:55720839-55720858	Msa0649130:five_prime_UTR	45.0%
	GCATTGTAGTCAAAGCCATG+TGG	-	chr4_3:55720902-55720921	Msa0649130:five_prime_UTR	45.0%
!	GCCTCTAGCTGGTTTAAACA+GGG	-	chr4_3:55720946-55720965	Msa0649130:five_prime_UTR	45.0%
	ACCCTGTTTAAACCAGCTAG+AGG	+	chr4_3:55720950-55720969	None:intergenic	45.0%
	TTAAACAGGGTCCTTTAGCG+AGG	-	chr4_3:55720959-55720978	Msa0649130:five_prime_UTR	45.0%
	CCTTTGAACCGAACGAAGAA+GGG	+	chr4_3:55719859-55719878	None:intergenic	45.0%
	CCTTCTTCGTTCGGTTCAAA+GGG	-	chr4_3:55719857-55719876	Msa0649130:intron	45.0%
	CAGGTACAAAATGGGACAGT+GGG	-	chr4_3:55720206-55720225	Msa0649130:intron	45.0%
	ATGATCACCATGGATGTGGA+CGG	-	chr4_3:55720563-55720582	Msa0649130:intron	45.0%
	ATTTATGCCGTCCACATCCA+TGG	+	chr4_3:55720573-55720592	None:intergenic	45.0%
	TGGATGTGGACGGCATAAAT+TGG	-	chr4_3:55720573-55720592	Msa0649130:intron	45.0%
	GGATGTGGACGGCATAAATT+GGG	-	chr4_3:55720574-55720593	Msa0649130:intron	45.0%
	ATATCATGTCTTCTGCCTCC+AGG	+	chr4_3:55720634-55720653	None:intergenic	45.0%
	GGCAGAAGACATGATATACG+AGG	-	chr4_3:55720637-55720656	Msa0649130:intron	45.0%
	AGGAAATGCAGCTCTACATG+CGG	+	chr4_3:55720837-55720856	None:intergenic	45.0%
	TGTAGAGCTGCATTTCCTCT+TGG	-	chr4_3:55720839-55720858	Msa0649130:five_prime_UTR	45.0%
	GCATTGTAGTCAAAGCCATG+TGG	-	chr4_3:55720902-55720921	Msa0649130:five_prime_UTR	45.0%
!	GCCTCTAGCTGGTTTAAACA+GGG	-	chr4_3:55720946-55720965	Msa0649130:five_prime_UTR	45.0%
	ACCCTGTTTAAACCAGCTAG+AGG	+	chr4_3:55720950-55720969	None:intergenic	45.0%
	TTAAACAGGGTCCTTTAGCG+AGG	-	chr4_3:55720959-55720978	Msa0649130:five_prime_UTR	45.0%
	CAAGGAAACGTGCACCTTGA+AGG	+	chr4_3:55719695-55719714	None:intergenic	50.0%
!	AGAAGAAGGGTACGAGGAAG+AGG	+	chr4_3:55719731-55719750	None:intergenic	50.0%
!	GACTTGAGAAGAAGGGTACG+AGG	+	chr4_3:55719737-55719756	None:intergenic	50.0%
	CCCTTCTTCGTTCGGTTCAA+AGG	-	chr4_3:55719856-55719875	Msa0649130:intron	50.0%
	CCCTTTGAACCGAACGAAGA+AGG	+	chr4_3:55719860-55719879	None:intergenic	50.0%
	GCAGGTACAAAATGGGACAG+TGG	-	chr4_3:55720205-55720224	Msa0649130:intron	50.0%
	GTGGACGGCATAAATTGGGT+TGG	-	chr4_3:55720578-55720597	Msa0649130:intron	50.0%
!	CGCCTCTAGCTGGTTTAAAC+AGG	-	chr4_3:55720945-55720964	Msa0649130:five_prime_UTR	50.0%
	CAAGGAAACGTGCACCTTGA+AGG	+	chr4_3:55719695-55719714	None:intergenic	50.0%
!	AGAAGAAGGGTACGAGGAAG+AGG	+	chr4_3:55719731-55719750	None:intergenic	50.0%
!	GACTTGAGAAGAAGGGTACG+AGG	+	chr4_3:55719737-55719756	None:intergenic	50.0%
	CCCTTCTTCGTTCGGTTCAA+AGG	-	chr4_3:55719856-55719875	Msa0649130:intron	50.0%
	CCCTTTGAACCGAACGAAGA+AGG	+	chr4_3:55719860-55719879	None:intergenic	50.0%
	GCAGGTACAAAATGGGACAG+TGG	-	chr4_3:55720205-55720224	Msa0649130:intron	50.0%
	GTGGACGGCATAAATTGGGT+TGG	-	chr4_3:55720578-55720597	Msa0649130:intron	50.0%
!	CGCCTCTAGCTGGTTTAAAC+AGG	-	chr4_3:55720945-55720964	Msa0649130:five_prime_UTR	50.0%
	GCTTCTGCTATTCTGGCGCT+AGG	+	chr4_3:55719667-55719686	None:intergenic	55.0%
	ACCTTCGGATGCTTGCCACA+TGG	+	chr4_3:55720920-55720939	None:intergenic	55.0%
	GCTTCTGCTATTCTGGCGCT+AGG	+	chr4_3:55719667-55719686	None:intergenic	55.0%
	ACCTTCGGATGCTTGCCACA+TGG	+	chr4_3:55720920-55720939	None:intergenic	55.0%
	GCAGCTCTACATGCGGCAGT+GGG	+	chr4_3:55720830-55720849	None:intergenic	60.0%
	TGCAGCTCTACATGCGGCAG+TGG	+	chr4_3:55720831-55720850	None:intergenic	60.0%
	GCCATGTGGCAAGCATCCGA+AGG	-	chr4_3:55720916-55720935	Msa0649130:five_prime_UTR	60.0%
!!	GCTAGAGGCGCTGAGACCTT+CGG	+	chr4_3:55720935-55720954	None:intergenic	60.0%
	AAGGTCTCAGCGCCTCTAGC+TGG	-	chr4_3:55720935-55720954	Msa0649130:five_prime_UTR	60.0%
	GCAGCTCTACATGCGGCAGT+GGG	+	chr4_3:55720830-55720849	None:intergenic	60.0%
	TGCAGCTCTACATGCGGCAG+TGG	+	chr4_3:55720831-55720850	None:intergenic	60.0%
	GCCATGTGGCAAGCATCCGA+AGG	-	chr4_3:55720916-55720935	Msa0649130:five_prime_UTR	60.0%
!!	GCTAGAGGCGCTGAGACCTT+CGG	+	chr4_3:55720935-55720954	None:intergenic	60.0%
	AAGGTCTCAGCGCCTCTAGC+TGG	-	chr4_3:55720935-55720954	Msa0649130:five_prime_UTR	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr4_3	gene	55719596	55720987	55719596	ID=Msa0649130;Name=Msa0649130
chr4_3	mRNA	55719596	55720987	55719596	ID=Msa0649130-mRNA-1;Parent=Msa0649130;Name=Msa0649130-mRNA-1;_AED=0.10;_eAED=0.10;_QI=350\|0.5\|0.66\|0.66\|0.5\|0.33\|3\|0\|68
chr4_3	exon	55719596	55719706	55719596	ID=Msa0649130-mRNA-1:exon:14356;Parent=Msa0649130-mRNA-1
chr4_3	exon	55719925	55720087	55719925	ID=Msa0649130-mRNA-1:exon:14355;Parent=Msa0649130-mRNA-1
chr4_3	exon	55720705	55720987	55720705	ID=Msa0649130-mRNA-1:exon:14354;Parent=Msa0649130-mRNA-1
chr4_3	five_prime_UTR	55720705	55720987	55720705	ID=Msa0649130-mRNA-1:five_prime_utr;Parent=Msa0649130-mRNA-1
chr4_3	five_prime_UTR	55720021	55720087	55720021	ID=Msa0649130-mRNA-1:five_prime_utr;Parent=Msa0649130-mRNA-1
chr4_3	CDS	55719925	55720020	55719925	ID=Msa0649130-mRNA-1:cds;Parent=Msa0649130-mRNA-1
chr4_3	CDS	55719596	55719706	55719596	ID=Msa0649130-mRNA-1:cds;Parent=Msa0649130-mRNA-1

Gene Sequence	>Msa0649130 ATGATCACCATGGATGTGGACGGCATAAATTGGGTTGGAAACTTGTATCAGAAATTTGAAAATATGTGCCTGGAGGCAGAAGACATGATATACGAGTATTCTGCCTTAATTATTGTTGTAAGCATTGTAGTCAAAGCCATGTGGCAAGCATCCGAAGGTCTCAGCGCCTCTAGCTGGTTTAAACAGGGTCCTTTAGCGAGGTATTAG
Protein sequence	>Msa0649130 MITMDVDGINWVGNLYQKFENMCLEAEDMIYEYSALIIVVSIVVKAMWQASEGLSASSWFKQGPLARY*