AlfalfaGEDB | Msa0161900

M. sativa cultivar zhongmu-4 / Msa0161900

KEGG: -
GO: -
PFAM: PF03101.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0161900	sp\|Q9SZL8\|FRS5_ARATH	36.242	149	74	6	2	132	21	166	6.14e-13	68.2

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0161900	A0A392N1M6	79.389	131	25	1	1	129	1	131	9.10e-70	215

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TFs/TRs:

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Gene ID	Type	Classification
Msa0161900	TF	FAR1

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0006110	Msa0161900	0.820403	6.988232e-53	-8.615850e-47
Msa0011170	Msa0161900	0.805568	1.253858e-49	-8.615850e-47
Msa0014710	Msa0161900	0.826657	2.404909e-54	-8.615850e-47
Msa0015390	Msa0161900	0.805230	1.475619e-49	-8.615850e-47
Msa0017070	Msa0161900	0.801929	7.140381e-49	-8.615850e-47
Msa0020410	Msa0161900	0.820938	5.264004e-53	-8.615850e-47
Msa0026910	Msa0161900	0.806487	8.030822e-50	-8.615850e-47
Msa0036140	Msa0161900	0.817479	3.228177e-52	-8.615850e-47
Msa0037880	Msa0161900	0.805736	1.155876e-49	-8.615850e-47
Msa0068740	Msa0161900	-0.802050	6.742326e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0161900	MtrunA17_Chr1g0202341	63.359	131	46	1	4	132	12	142	1.67e-50	170
Msa0161900	MtrunA17_Chr3g0077241	70.652	92	21	2	6	94	1	89	1.55e-39	129
Msa0161900	MtrunA17_Chr4g0052331	57.895	95	38	1	1	93	1	95	3.29e-33	112
Msa0161900	MtrunA17_Chr2g0329361	38.947	95	57	1	39	132	59	153	3.37e-18	78.2
Msa0161900	MtrunA17_Chr2g0329421	33.333	117	66	4	23	132	5	116	7.27e-16	71.2
Msa0161900	MtrunA17_Chr4g0033611	32.000	125	70	4	19	133	47	166	2.99e-15	70.1
Msa0161900	MtrunA17_Chr5g0448761	45.918	98	48	3	39	132	58	154	2.04e-13	66.2
Msa0161900	MtrunA17_Chr3g0078041	35.789	95	57	3	40	132	49	141	2.89e-12	61.6
Msa0161900	MtrunA17_Chr2g0287061	31.068	103	55	2	31	132	173	260	3.70e-12	62.8
Msa0161900	MtrunA17_Chr6g0458391	36.111	108	51	3	36	140	86	178	2.03e-11	60.5

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0161900	AT3G59470.3	36.170	94	56	2	40	132	69	159	2.88e-15	70.5
Msa0161900	AT3G59470.1	36.170	94	56	2	40	132	69	159	2.88e-15	70.5
Msa0161900	AT3G59470.2	36.170	94	56	2	40	132	81	171	3.21e-15	70.5
Msa0161900	AT4G38180.1	36.242	149	74	6	2	132	21	166	6.24e-14	68.2
Msa0161900	AT3G06250.2	35.789	95	45	3	39	132	26	105	7.08e-13	65.1
Msa0161900	AT3G06250.1	35.789	95	45	3	39	132	26	105	7.08e-13	65.1
Msa0161900	AT3G06250.3	35.789	95	45	3	39	132	26	105	7.08e-13	65.1
Msa0161900	AT3G07500.1	38.947	95	55	2	39	132	31	123	2.45e-12	62.0
Msa0161900	AT5G18960.2	33.684	95	47	3	39	132	41	120	3.89e-12	62.8
Msa0161900	AT5G18960.1	33.684	95	47	3	39	132	41	120	3.89e-12	62.8

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Find 39 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATACTACACAAGGTACGCG+AGG	0.651596	1_4:+38015980	Msa0161900:CDS
AATGGTAGTCTATCATTGAG+AGG	0.621900	1_4:-38015898	None:intergenic
AATGGTAGTCTTATTGTACA+TGG	0.595875	1_4:+38016126	Msa0161900:CDS
ACAAGGTACGCGAGGAATAA+AGG	0.387113	1_4:+38015988	Msa0161900:CDS
ACCGTCTTCGAAGAAGATTA+TGG	0.460480	1_4:+38015507	Msa0161900:five_prime_UTR
ACTGTTTGTGTTGAAGCTGC+AGG	0.548752	1_4:+38016207	Msa0161900:CDS
AGAACGGATGGTAGAGTAAT+TGG	0.359499	1_4:+38016045	Msa0161900:CDS
AGACGGTTCTCAGGTCGTAA+CGG	0.630551	1_4:-38015491	None:intergenic
AGAGATTCTTTGTTCAAAGG+AGG	0.550163	1_4:+38016071	Msa0161900:CDS
AGATTCTTTGTTCAAAGGAG+GGG	0.523644	1_4:+38016073	Msa0161900:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TCAAACGAACAAAAGAAAGT+AGG	-	chr1_4:38015439-38015458	None:intergenic	30.0%
TTGTAGAACTAAAGAAACCA+AGG	-	chr1_4:38015585-38015604	None:intergenic	30.0%
CATTGCCAAACAAAAATCTT+TGG	-	chr1_4:38015650-38015669	None:intergenic	30.0%
TCAAGAGATTCTTTGTTCAA+AGG	+	chr1_4:38016068-38016087	Msa0161900:CDS	30.0%
TTATTGTACATGGTCAAACA+AGG	+	chr1_4:38016136-38016155	Msa0161900:CDS	30.0%
TATTGTACATGGTCAAACAA+GGG	+	chr1_4:38016137-38016156	Msa0161900:CDS	30.0%
TCAAACGAACAAAAGAAAGT+AGG	-	chr1_4:38015439-38015458	None:intergenic	30.0%
TTGTAGAACTAAAGAAACCA+AGG	-	chr1_4:38015585-38015604	None:intergenic	30.0%
CATTGCCAAACAAAAATCTT+TGG	-	chr1_4:38015650-38015669	None:intergenic	30.0%
TCAAGAGATTCTTTGTTCAA+AGG	+	chr1_4:38016068-38016087	Msa0161900:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1_4	gene	38015347	38016248	38015347	ID=Msa0161900;Name=Msa0161900
chr1_4	mRNA	38015347	38016248	38015347	ID=Msa0161900-mRNA-1;Parent=Msa0161900;Name=Msa0161900-mRNA-1;_AED=0.11;_eAED=0.13;_QI=235\|1\|0.5\|1\|0\|0.5\|2\|0\|143
chr1_4	exon	38015347	38015557	38015347	ID=Msa0161900-mRNA-1:exon:11144;Parent=Msa0161900-mRNA-1
chr1_4	exon	38015793	38016248	38015793	ID=Msa0161900-mRNA-1:exon:11145;Parent=Msa0161900-mRNA-1
chr1_4	five_prime_UTR	38015347	38015557	38015347	ID=Msa0161900-mRNA-1:five_prime_utr;Parent=Msa0161900-mRNA-1
chr1_4	five_prime_UTR	38015793	38015816	38015793	ID=Msa0161900-mRNA-1:five_prime_utr;Parent=Msa0161900-mRNA-1
chr1_4	CDS	38015817	38016248	38015817	ID=Msa0161900-mRNA-1:cds;Parent=Msa0161900-mRNA-1

Gene Sequence

>Msa0161900

ATGACAGATCAAGAAATGACAGATATGCATGAACATACCAACATGATTGGCTCAGATGATATGCTTGCAAGTGATGCAGATCCTCTCAATGATAGACTACCATTGGAGCTCCCCTTAGAGCCCTTTGAAGGAATGGAGTTTACGTCAATTGAAGATGTAAAAAAATACTACACAAGGTACGCGAGGAATAAAGGTTTTAGCTTTCGTATGGGTCGTGTTACTAAATCAAGAACGGATGGTAGAGTAATTGGTCAAGAGATTCTTTGTTCAAAGGAGGGGTTTAGATCCCAAAAAAATTTGAAAAAAGAAAATGGTAGTCTTATTGTACATGGTCAAACAAGGGTAGGATGCAAGGCACTCTTGTATATCAAGAAAAGTGAAGATAGGTGGACTGTTTGTGTTGAAGCTGCAGGCAGGTTGTTTTTAATCTGA

Protein sequence

>Msa0161900

MTDQEMTDMHEHTNMIGSDDMLASDADPLNDRLPLELPLEPFEGMEFTSIEDVKKYYTRYARNKGFSFRMGRVTKSRTDGRVIGQEILCSKEGFRSQKNLKKENGSLIVHGQTRVGCKALLYIKKSEDRWTVCVEAAGRLFLI*