AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1120610


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1120610 MtrunA17_Chr7g0251061 79.741 232 24 2 1 209 33 264 2.74e-126 370
Msa1120610 MtrunA17_Chr7g0251121 57.872 235 67 10 1 209 29 257 1.18e-80 253
Msa1120610 MtrunA17_Chr7g0251101 57.872 235 67 10 1 209 33 261 5.32e-80 251
Msa1120610 MtrunA17_Chr7g0251091 77.124 153 20 3 1 138 1 153 7.02e-77 228
Msa1120610 MtrunA17_Chr7g0251071 54.321 243 72 12 1 209 32 269 1.20e-72 232
Msa1120610 MtrunA17_Chr7g0251051 52.752 218 67 6 1 205 31 225 2.57e-65 212
Msa1120610 MtrunA17_Chr1g0212811 45.492 244 90 12 1 208 20 256 2.44e-52 169
Msa1120610 MtrunA17_Chr7g0251081 100.000 38 0 0 172 209 2 39 9.01e-20 86.3
Msa1120610 MtrunA17_Chr7g0251131 29.954 217 112 9 11 198 32 237 2.64e-18 80.5
Msa1120610 MtrunA17_Chr7g0251011 29.954 217 112 9 11 198 35 240 3.69e-18 80.5
Msa1120610 MtrunA17_Chr8g0376061 28.261 230 129 10 6 206 27 249 4.04e-17 79.3
Msa1120610 MtrunA17_Chr8g0391231 26.230 244 133 14 3 209 24 257 6.27e-13 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1120610 AT1G18390.1 28.070 228 128 11 5 205 25 243 4.43e-14 70.9
Msa1120610 AT1G66930.2 26.768 198 112 7 11 186 33 219 6.12e-12 64.7

Find 28 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAAATGGATGGGAAATTA+TGG 0.249832 7_3:+70122990 Msa1120610:CDS
TTATCGAATGTGCTGTTCTT+AGG 0.305690 7_3:-70122717 None:intergenic
ACTTCGTTGATATTTATCTT+TGG 0.325710 7_3:-70122594 None:intergenic
TGAACATCAAGTATCCATTC+TGG 0.331522 7_3:+70122496 Msa1120610:CDS
CAGTCGCCAGAGATGATTAC+TGG 0.372478 7_3:+70122661 Msa1120610:CDS
TTTAGATGTTTCTGCAAAGA+TGG 0.375944 7_3:+70123074 Msa1120610:CDS
TCGCATGTAAGATCTATGTT+AGG 0.426387 7_3:-70122561 None:intergenic
CGTTGTTGCAGTTTGTGTAT+TGG 0.453733 7_3:-70122449 None:intergenic
ATATTGTGGTGGTGTTTCTT+CGG 0.454897 7_3:+70122536 Msa1120610:CDS
ATTGAGCGTTGCAATTTGTC+CGG 0.459800 7_3:-70122934 None:intergenic
GAACATCAAGTATCCATTCT+GGG 0.486266 7_3:+70122497 Msa1120610:CDS
ACCACTCCAGTAATCATCTC+TGG 0.493007 7_3:-70122667 None:intergenic
AACTTCATGTGCCTCACAAA+AGG 0.494156 7_3:+70123106 Msa1120610:CDS
GTTGATTCTGATGGTGTATG+TGG 0.513530 7_3:+70123029 Msa1120610:CDS
AACACTATGTTTCAAAGTGA+TGG 0.528238 7_3:+70122738 Msa1120610:CDS
CATGTGAGTGAGACGGAAGC+AGG 0.529693 7_3:-70122867 None:intergenic
TAAAGTTTCCTACACAATTG+TGG 0.540640 7_3:+70122842 Msa1120610:CDS
GATATAAACGAAGCATTACA+AGG 0.553914 7_3:+70122957 Msa1120610:CDS
CAAAAGTGTGTTGATTCTGA+TGG 0.579264 7_3:+70123020 Msa1120610:CDS
GGAAACTTTAGTATTACCGC+AGG 0.610021 7_3:-70122829 None:intergenic
GCCAGAGATGATTACTGGAG+TGG 0.616913 7_3:+70122666 Msa1120610:CDS
AGCAGGATCCACAATTGTGT+AGG 0.653468 7_3:-70122850 None:intergenic
CGGAGTACATGTGAGTGAGA+CGG 0.655647 7_3:-70122874 None:intergenic
AACATCAAGTATCCATTCTG+GGG 0.658534 7_3:+70122498 Msa1120610:CDS
GGTGTATGTGGAAGCAACGA+CGG 0.699910 7_3:+70123041 Msa1120610:CDS
ACTATGTTTCAAAGTGATGG+TGG 0.706305 7_3:+70122741 Msa1120610:CDS
GTATGTGGAAGCAACGACGG+AGG 0.741380 7_3:+70123044 Msa1120610:CDS
ATAAACGAAGCATTACAAGG+CGG 0.787280 7_3:+70122960 Msa1120610:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAAAATAGAAAAAAATATTG+TGG + chr7_3:70122522-70122541 Msa1120610:CDS 15.0%
!! AATAGAAAAAAATATTGTGG+TGG + chr7_3:70122525-70122544 Msa1120610:CDS 20.0%
! ACTTCGTTGATATTTATCTT+TGG - chr7_3:70122597-70122616 None:intergenic 25.0%
! TATCATTCCAATCAAGAATA+CGG - chr7_3:70122626-70122645 None:intergenic 25.0%
!!! AATATTTTTAGTACTACCTG+CGG + chr7_3:70122813-70122832 Msa1120610:CDS 25.0%
!! TATTGTTGAGTTTCCAATTT+TGG + chr7_3:70122902-70122921 Msa1120610:intron 25.0%
TCAAATACCGTATTCTTGAT+TGG + chr7_3:70122616-70122635 Msa1120610:intron 30.0%
AACACTATGTTTCAAAGTGA+TGG + chr7_3:70122738-70122757 Msa1120610:CDS 30.0%
TAAAGTTTCCTACACAATTG+TGG + chr7_3:70122842-70122861 Msa1120610:CDS 30.0%
! GATATAAACGAAGCATTACA+AGG + chr7_3:70122957-70122976 Msa1120610:CDS 30.0%
!!! GGTTTTGATTTGAAATGGAT+GGG + chr7_3:70122981-70123000 Msa1120610:CDS 30.0%
TTGAAATGGATGGGAAATTA+TGG + chr7_3:70122990-70123009 Msa1120610:CDS 30.0%
! TTTAGATGTTTCTGCAAAGA+TGG + chr7_3:70123074-70123093 Msa1120610:CDS 30.0%
TGAACATCAAGTATCCATTC+TGG + chr7_3:70122496-70122515 Msa1120610:CDS 35.0%
GAACATCAAGTATCCATTCT+GGG + chr7_3:70122497-70122516 Msa1120610:CDS 35.0%
AACATCAAGTATCCATTCTG+GGG + chr7_3:70122498-70122517 Msa1120610:CDS 35.0%
! TTTCTATTTTCTCCCCAGAA+TGG - chr7_3:70122513-70122532 None:intergenic 35.0%
! ATATTGTGGTGGTGTTTCTT+CGG + chr7_3:70122536-70122555 Msa1120610:CDS 35.0%
TCGCATGTAAGATCTATGTT+AGG - chr7_3:70122564-70122583 None:intergenic 35.0%
! TTATCGAATGTGCTGTTCTT+AGG - chr7_3:70122720-70122739 None:intergenic 35.0%
ACTATGTTTCAAAGTGATGG+TGG + chr7_3:70122741-70122760 Msa1120610:CDS 35.0%
GGAAACTCAACAATAACACT+CGG - chr7_3:70122897-70122916 None:intergenic 35.0%
! ATAAACGAAGCATTACAAGG+CGG + chr7_3:70122960-70122979 Msa1120610:CDS 35.0%
!!! AAGGCGGTTTTGATTTGAAA+TGG + chr7_3:70122976-70122995 Msa1120610:CDS 35.0%
!!! CGGTTTTGATTTGAAATGGA+TGG + chr7_3:70122980-70122999 Msa1120610:CDS 35.0%
!! CAAAAGTGTGTTGATTCTGA+TGG + chr7_3:70123020-70123039 Msa1120610:CDS 35.0%
CGTTGTTGCAGTTTGTGTAT+TGG - chr7_3:70122452-70122471 None:intergenic 40.0%
!! TGCAACAACGCTTTTAACTG+CGG + chr7_3:70122462-70122481 Msa1120610:CDS 40.0%
GGAAACTTTAGTATTACCGC+AGG - chr7_3:70122832-70122851 None:intergenic 40.0%
! ATTGAGCGTTGCAATTTGTC+CGG - chr7_3:70122937-70122956 None:intergenic 40.0%
! GTTGATTCTGATGGTGTATG+TGG + chr7_3:70123029-70123048 Msa1120610:CDS 40.0%
ACCACTCCAGTAATCATCTC+TGG - chr7_3:70122670-70122689 None:intergenic 45.0%
AGCAGGATCCACAATTGTGT+AGG - chr7_3:70122853-70122872 None:intergenic 45.0%
!! CCAATTTTGGCAGTCAAAGC+CGG + chr7_3:70122915-70122934 Msa1120610:intron 45.0%
CAGTCGCCAGAGATGATTAC+TGG + chr7_3:70122661-70122680 Msa1120610:CDS 50.0%
GCCAGAGATGATTACTGGAG+TGG + chr7_3:70122666-70122685 Msa1120610:CDS 50.0%
CGGAGTACATGTGAGTGAGA+CGG - chr7_3:70122877-70122896 None:intergenic 50.0%
! CCGGCTTTGACTGCCAAAAT+TGG - chr7_3:70122918-70122937 None:intergenic 50.0%
GGTGTATGTGGAAGCAACGA+CGG + chr7_3:70123041-70123060 Msa1120610:CDS 50.0%
CATGTGAGTGAGACGGAAGC+AGG - chr7_3:70122870-70122889 None:intergenic 55.0%
GTATGTGGAAGCAACGACGG+AGG + chr7_3:70123044-70123063 Msa1120610:CDS 55.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 70122432 70123127 70122432 ID=Msa1120610;Name=Msa1120610
chr7_3 mRNA 70122432 70123127 70122432 ID=Msa1120610-mRNA-1;Parent=Msa1120610;Name=Msa1120610-mRNA-1;_AED=0.44;_eAED=0.44;_QI=0|0|0|1|0.5|0.66|3|0|209
chr7_3 exon 70122432 70122614 70122432 ID=Msa1120610-mRNA-1:exon:17855;Parent=Msa1120610-mRNA-1
chr7_3 exon 70122654 70122897 70122654 ID=Msa1120610-mRNA-1:exon:17856;Parent=Msa1120610-mRNA-1
chr7_3 exon 70122928 70123127 70122928 ID=Msa1120610-mRNA-1:exon:17857;Parent=Msa1120610-mRNA-1
chr7_3 CDS 70122432 70122614 70122432 ID=Msa1120610-mRNA-1:cds;Parent=Msa1120610-mRNA-1
chr7_3 CDS 70122654 70122897 70122654 ID=Msa1120610-mRNA-1:cds;Parent=Msa1120610-mRNA-1
chr7_3 CDS 70122928 70123127 70122928 ID=Msa1120610-mRNA-1:cds;Parent=Msa1120610-mRNA-1
Gene Sequence

>Msa1120610

ATGTGCGATGATGATGACCAATACACAAACTGCAACAACGCTTTTAACTGCGGAAGCAGCATCGTGAACATCAAGTATCCATTCTGGGGAGAAAATAGAAAAAAATATTGTGGTGGTGTTTCTTCGGATCCTAACATAGATCTTACATGCGAAGAAAGTGTTCCAAAGATAAATATCAACGAAAAACTCACAGTCGCCAGAGATGATTACTGGAGTGGTATTTGTGCTGTGAATGTGAGTAGCAATCCTAAGAACAGCACATTCGATAACACTATGTTTCAAAGTGATGGTGGTGTTTCTTCTCAAGTAAATCTGTTATACAATTGTGATGCAAGTACAATGAATATTTTTAGTACTACCTGCGGTAATACTAAAGTTTCCTACACAATTGTGGATCCTGCTTCCGTCTCACTCACATGTACTCCGATCAAAGCCGGACAAATTGCAACGCTCAATGATATAAACGAAGCATTACAAGGCGGTTTTGATTTGAAATGGATGGGAAATTATGGAGAGTGTCAAAAGTGTGTTGATTCTGATGGTGTATGTGGAAGCAACGACGGAGGAAATGAATTTAGATGTTTCTGCAAAGATGGATCTCATACAACTTCATGTGCCTCACAAAAG

Protein sequence

>Msa1120610

MCDDDDQYTNCNNAFNCGSSIVNIKYPFWGENRKKYCGGVSSDPNIDLTCEESVPKININEKLTVARDDYWSGICAVNVSSNPKNSTFDNTMFQSDGGVSSQVNLLYNCDASTMNIFSTTCGNTKVSYTIVDPASVSLTCTPIKAGQIATLNDINEALQGGFDLKWMGNYGECQKCVDSDGVCGSNDGGNEFRCFCKDGSHTTSCASQK