AlfalfaGEDB | Msa1218590

M. sativa cultivar zhongmu-4 / Msa1218590

KEGG: K02905
GO: GO:0000003 GO:0000184 GO:0000956 GO:0002181 GO:0003674 GO:0003682 GO:0003735 GO:0005198 GO:0005488 GO:0005539 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005783 GO:0005789 GO:0005791 GO:0005829 GO:0005840 GO:0005844 GO:0006139 GO:0006401 GO:0006402 GO:0006412 GO:0006413 GO:0006518 GO:0006605 GO:0006612 GO:0006613 GO:0006614 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0007155 GO:0007275 GO:0007565 GO:0007566 GO:0007568 GO:0008104 GO:0008150 GO:0008152 GO:0008201 GO:0008283 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009892 GO:0009987 GO:0010467 GO:0010468 GO:0010605 GO:0010629 GO:0012505 GO:0015031 GO:0015833 GO:0015934 GO:0016020 GO:0016070 GO:0016071 GO:0019222 GO:0019439 GO:0019538 GO:0019898 GO:0022414 GO:0022610 GO:0022625 GO:0022626 GO:0030867 GO:0031090 GO:0031589 GO:0031974 GO:0031981 GO:0031984 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0034641 GO:0034645 GO:0034655 GO:0035264 GO:0040007 GO:0042175 GO:0042788 GO:0042886 GO:0043043 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044265 GO:0044267 GO:0044270 GO:0044271 GO:0044391 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044432 GO:0044444 GO:0044445 GO:0044446 GO:0044464 GO:0044703 GO:0044706 GO:0045047 GO:0045184 GO:0046483 GO:0046700 GO:0046907 GO:0048519 GO:0048589 GO:0048856 GO:0050789 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051704 GO:0060255 GO:0065007 GO:0070013 GO:0070727 GO:0070972 GO:0071702 GO:0071704 GO:0071705 GO:0072594 GO:0072599 GO:0072657 GO:0090150 GO:0090304 GO:0097367 GO:0098552 GO:0098554 GO:0098556 GO:0098562 GO:0098588 GO:0098827 GO:1901360 GO:1901361 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1901681 GO:1990904
PFAM: PF01779.20

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1218590	XP_004508161.1	100.000	61	0	0	1	61	1	61	1.11e-35	124

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1218590	sp\|Q9M7X7\|RL291_ARATH	85.246	61	9	0	1	61	1	61	3.96e-31	105

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0004540	Msa1218590	0.800854	1.185471e-48	-8.615850e-47
Msa0008410	Msa1218590	0.836924	7.036114e-57	-8.615850e-47
Msa0044020	Msa1218590	0.817413	3.340251e-52	-8.615850e-47
Msa0057710	Msa1218590	0.811704	6.128205e-51	-8.615850e-47
Msa0064350	Msa1218590	0.803846	2.868956e-49	-8.615850e-47
Msa0069330	Msa1218590	0.812818	3.500567e-51	-8.615850e-47
Msa0073670	Msa1218590	0.828557	8.413475e-55	-8.615850e-47
Msa0076040	Msa1218590	0.815832	7.552710e-52	-8.615850e-47
Msa0104550	Msa1218590	0.806029	1.003099e-49	-8.615850e-47
Msa0639120	Msa1218590	0.816053	6.741318e-52	-8.615850e-47
Msa0645170	Msa1218590	0.803280	3.759552e-49	-8.615850e-47
Msa0663900	Msa1218590	0.817472	3.239252e-52	-8.615850e-47
Msa0684500	Msa1218590	0.806734	7.125010e-50	-8.615850e-47
Msa0687630	Msa1218590	0.813028	3.148795e-51	-8.615850e-47
Msa0692840	Msa1218590	0.821523	3.860061e-53	-8.615850e-47
Msa0703230	Msa1218590	0.812502	4.104834e-51	-8.615850e-47
Msa0734960	Msa1218590	0.823099	1.662508e-53	-8.615850e-47
Msa0741900	Msa1218590	0.821173	4.648133e-53	-8.615850e-47
Msa0749760	Msa1218590	0.805354	1.389916e-49	-8.615850e-47
Msa0750880	Msa1218590	0.803761	2.987012e-49	-8.615850e-47
Msa0755340	Msa1218590	0.813960	1.964829e-51	-8.615850e-47
Msa0761940	Msa1218590	0.808844	2.536861e-50	-8.615850e-47
Msa0784380	Msa1218590	0.803356	3.624631e-49	-8.615850e-47
Msa0814320	Msa1218590	0.808848	2.531298e-50	-8.615850e-47
Msa0822990	Msa1218590	0.803336	3.659525e-49	-8.615850e-47
Msa0124340	Msa1218590	0.801254	9.817896e-49	-8.615850e-47
Msa0130240	Msa1218590	0.804701	1.904111e-49	-8.615850e-47
Msa0140300	Msa1218590	0.820361	7.144898e-53	-8.615850e-47
Msa0142950	Msa1218590	0.802600	5.195712e-49	-8.615850e-47
Msa0143380	Msa1218590	0.803788	2.948666e-49	-8.615850e-47
Msa0148100	Msa1218590	0.806927	6.483625e-50	-8.615850e-47
Msa0181610	Msa1218590	0.809121	2.213154e-50	-8.615850e-47
Msa0185590	Msa1218590	0.804260	2.352556e-49	-8.615850e-47
Msa0186890	Msa1218590	0.811222	7.798078e-51	-8.615850e-47
Msa0197930	Msa1218590	0.800925	1.146563e-48	-8.615850e-47
Msa0199690	Msa1218590	0.800030	1.744946e-48	-8.615850e-47
Msa0413450	Msa1218590	0.817930	2.553990e-52	-8.615850e-47
Msa0415810	Msa1218590	0.801362	9.333510e-49	-8.615850e-47
Msa0428170	Msa1218590	0.820141	8.022356e-53	-8.615850e-47
Msa0462990	Msa1218590	0.811518	6.726632e-51	-8.615850e-47
Msa0980940	Msa1218590	0.823122	1.641567e-53	-8.615850e-47
Msa1004420	Msa1218590	0.814599	1.419000e-51	-8.615850e-47
Msa1013770	Msa1218590	0.803977	2.694089e-49	-8.615850e-47
Msa1055010	Msa1218590	0.802781	4.766533e-49	-8.615850e-47
Msa1093840	Msa1218590	0.807306	5.391383e-50	-8.615850e-47
Msa0239450	Msa1218590	0.812978	3.230302e-51	-8.615850e-47
Msa0311700	Msa1218590	0.804081	2.563029e-49	-8.615850e-47
Msa0333920	Msa1218590	0.800658	1.299934e-48	-8.615850e-47
Msa0334040	Msa1218590	0.800254	1.571064e-48	-8.615850e-47
Msa0355270	Msa1218590	0.801181	1.016499e-48	-8.615850e-47
Msa1126510	Msa1218590	0.804779	1.834122e-49	-8.615850e-47
Msa1126770	Msa1218590	0.819900	9.107052e-53	-8.615850e-47
Msa1127890	Msa1218590	0.826735	2.304853e-54	-8.615850e-47
Msa1134110	Msa1218590	0.807061	6.075476e-50	-8.615850e-47
Msa1139810	Msa1218590	0.809041	2.302014e-50	-8.615850e-47
Msa1140690	Msa1218590	0.802535	5.357632e-49	-8.615850e-47
Msa1165870	Msa1218590	0.821091	4.853557e-53	-8.615850e-47
Msa1174100	Msa1218590	0.823995	1.025823e-53	-8.615850e-47
Msa1183660	Msa1218590	0.800893	1.163946e-48	-8.615850e-47
Msa1184370	Msa1218590	0.819877	9.220060e-53	-8.615850e-47
Msa1187840	Msa1218590	0.800079	1.705551e-48	-8.615850e-47
Msa1201830	Msa1218590	0.822788	1.963868e-53	-8.615850e-47
Msa1218590	Msa1218600	0.818481	1.916269e-52	-8.615850e-47
Msa1218590	Msa1227690	0.824308	8.659975e-54	-8.615850e-47
Msa1218590	Msa1228890	0.809354	1.972491e-50	-8.615850e-47
Msa1218590	Msa1235250	0.810726	9.988415e-51	-8.615850e-47
Msa1218590	Msa1251160	0.814139	1.793953e-51	-8.615850e-47
Msa1218590	Msa1254710	0.836958	6.896425e-57	-8.615850e-47
Msa1218590	Msa1294840	0.823888	1.086992e-53	-8.615850e-47
Msa1218590	Msa1295110	0.801847	7.423442e-49	-8.615850e-47
Msa1218590	Msa1303250	0.810505	1.114878e-50	-8.615850e-47
Msa1218590	Msa1307950	0.822450	2.353451e-53	-8.615850e-47
Msa1218590	Msa1331910	0.800592	1.340885e-48	-8.615850e-47
Msa1218590	Msa1344850	0.823069	1.688900e-53	-8.615850e-47
Msa1218590	Msa1353060	0.803002	4.290143e-49	-8.615850e-47
Msa1218590	Msa1365150	0.826560	2.537168e-54	-8.615850e-47
Msa1218590	Msa1367880	0.828806	7.326281e-55	-8.615850e-47
Msa1218590	Msa1398860	0.807630	4.602345e-50	-8.615850e-47
Msa1218590	Msa1404190	0.811209	7.849495e-51	-8.615850e-47
Msa1218590	Msa1438640	0.822615	2.154448e-53	-8.615850e-47
Msa1218590	Msa1443930	0.803974	2.697870e-49	-8.615850e-47
Msa1218590	Msa1452480	0.800592	1.340885e-48	-8.615850e-47
Msa1218590	Msa1465080	0.812158	4.880010e-51	-8.615850e-47
Msa0541860	Msa1218590	0.811313	7.451160e-51	-8.615850e-47
Msa0552810	Msa1218590	0.833829	4.258225e-56	-8.615850e-47
Msa0560470	Msa1218590	0.829329	5.471330e-55	-8.615850e-47
Msa0577220	Msa1218590	0.803244	3.824149e-49	-8.615850e-47
Msa0581070	Msa1218590	0.815217	1.035233e-51	-8.615850e-47
Msa0850150	Msa1218590	0.809423	1.906582e-50	-8.615850e-47
Msa0863370	Msa1218590	0.810696	1.013596e-50	-8.615850e-47
Msa0864010	Msa1218590	0.800442	1.438253e-48	-8.615850e-47
Msa0869100	Msa1218590	0.804277	2.333886e-49	-8.615850e-47
Msa0872310	Msa1218590	0.803392	3.563421e-49	-8.615850e-47
Msa0888940	Msa1218590	0.817963	2.510070e-52	-8.615850e-47
Msa0898760	Msa1218590	0.800436	1.442234e-48	-8.615850e-47
Msa0930780	Msa1218590	0.821280	4.390504e-53	-8.615850e-47
Msa0936410	Msa1218590	0.814341	1.618550e-51	-8.615850e-47
Msa0937550	Msa1218590	0.806313	8.741810e-50	-8.615850e-47
Msa0944660	Msa1218590	0.803057	4.179507e-49	-8.615850e-47
Msa0967370	Msa1218590	0.826958	2.038028e-54	-8.615850e-47
Msa0973250	Msa1218590	0.802616	5.156595e-49	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1218590	MtrunA17_Chr4g0068191	100.000	61	0	0	1	61	1	61	1.02e-39	124
Msa1218590	MtrunA17_Chr4g0068181	95.082	61	3	0	1	61	1	61	4.04e-38	120

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa1218590	AT3G06680.1	85.246	61	9	1	61	23	83	3.55e-32	106
Msa1218590	AT3G06700.1	85.246	61	9	1	61	1	61	4.02e-32	105
Msa1218590	AT3G06700.2	85.246	61	9	1	61	1	61	4.02e-32	105
Msa1218590	AT3G06700.3	85.246	61	9	1	61	1	61	4.02e-32	105
Msa1218590	AT3G06680.2	85.246	61	9	1	61	1	61	8.88e-32	104
Msa1218590	AT3G06680.3	85.246	61	9	1	61	1	61	8.88e-32	104

Find 17 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGTAAATTGTTAATCTATAA+TGG	0.244329	8_1:+81231910	Msa1218590:exon
GTAAATTGTTAATCTATAAT+GGG	0.337865	8_1:+81231911	Msa1218590:exon
GAAGTGTGGCGATGCCTCTT+TGG	0.338170	8_1:-81231672	None:intergenic
TCTTGTGAGCCTTGTAAGAC+TGG	0.428046	8_1:-81231635	None:intergenic
TGTGCTTCCTTGCATACCTC+TGG	0.438138	8_1:-81231854	None:intergenic
CGCTCACAACCAGTCTTACA+AGG	0.489194	8_1:+81231626	Msa1218590:CDS
TTAACATAACAAATCAAAGA+TGG	0.518906	8_1:-81231947	None:intergenic
TCTTACAAGGCTCACAAGAA+TGG	0.521704	8_1:+81231639	Msa1218590:CDS
TAAATTGTTAATCTATAATG+GGG	0.559815	8_1:+81231912	Msa1218590:exon
CATCGCCACACTTCCACCAA+GGG	0.564531	8_1:+81231681	Msa1218590:CDS
AACAATTTACTCTTCTTCAG+TGG	0.567212	8_1:-81231898	None:intergenic
TGAGGAACCAGAGGTATGCA+AGG	0.602234	8_1:+81231847	Msa1218590:CDS
GCATCGCCACACTTCCACCA+AGG	0.609525	8_1:+81231680	Msa1218590:CDS
ATGGCATCAAGAAGCCAAAG+AGG	0.631544	8_1:+81231658	Msa1218590:CDS
ATCGCCACACTTCCACCAAG+GGG	0.644272	8_1:+81231682	Msa1218590:CDS
CTTGTAAGACTGGTTGTGAG+CGG	0.674336	8_1:-81231625	None:intergenic
TCGCCACACTTCCACCAAGG+GGG	0.721304	8_1:+81231683	Msa1218590:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AGTAAATTGTTAATCTATAA+TGG	+	chr8_1:81231910-81231929	Msa1218590:exon	15.0%
!!	GTAAATTGTTAATCTATAAT+GGG	+	chr8_1:81231911-81231930	Msa1218590:exon	15.0%
!!	TAAATTGTTAATCTATAATG+GGG	+	chr8_1:81231912-81231931	Msa1218590:exon	15.0%
!!	TTAACATAACAAATCAAAGA+TGG	-	chr8_1:81231950-81231969	None:intergenic	20.0%
!	ATGAAAAAATGAGAAAGACA+AGG	-	chr8_1:81231726-81231745	None:intergenic	25.0%
!!!	TTTTTGATGATAACTGTAGA+TGG	+	chr8_1:81231812-81231831	Msa1218590:intron	25.0%
	AACAATTTACTCTTCTTCAG+TGG	-	chr8_1:81231901-81231920	None:intergenic	30.0%
	TAAACAAAAACCAGCAAAAC+TGG	-	chr8_1:81232026-81232045	None:intergenic	30.0%
!	AGATGGATCCAAAGTTTTTG+AGG	+	chr8_1:81231829-81231848	Msa1218590:intron	35.0%
!	AGGAAGCACAACAAGAAAAA+CGG	+	chr8_1:81231867-81231886	Msa1218590:CDS	35.0%
	TCTTACAAGGCTCACAAGAA+TGG	+	chr8_1:81231639-81231658	Msa1218590:CDS	40.0%
	AAGAGGAATAATTACCCCCT+TGG	-	chr8_1:81231700-81231719	None:intergenic	40.0%
	TGAGAAAGACAAGGAGAAAG+AGG	-	chr8_1:81231717-81231736	None:intergenic	40.0%
!	CTCTGGTTCCTCAAAAACTT+TGG	-	chr8_1:81231840-81231859	None:intergenic	40.0%
!!	CAAAGTTTTTGAGGAACCAG+AGG	+	chr8_1:81231838-81231857	Msa1218590:CDS	40.0%
!	CTTGTAAGACTGGTTGTGAG+CGG	-	chr8_1:81231628-81231647	None:intergenic	45.0%
!	TCTTGTGAGCCTTGTAAGAC+TGG	-	chr8_1:81231638-81231657	None:intergenic	45.0%
	ATGGCATCAAGAAGCCAAAG+AGG	+	chr8_1:81231658-81231677	Msa1218590:CDS	45.0%
	AGGAATAATTACCCCCTTGG+TGG	-	chr8_1:81231697-81231716	None:intergenic	45.0%
!	TACTCTGTTGCCAGTTTTGC+TGG	+	chr8_1:81232013-81232032	Msa1218590:three_prime_UTR	45.0%
	CGCTCACAACCAGTCTTACA+AGG	+	chr8_1:81231626-81231645	Msa1218590:CDS	50.0%
!	TGAGGAACCAGAGGTATGCA+AGG	+	chr8_1:81231847-81231866	Msa1218590:CDS	50.0%
	TGTGCTTCCTTGCATACCTC+TGG	-	chr8_1:81231857-81231876	None:intergenic	50.0%
	GAAGTGTGGCGATGCCTCTT+TGG	-	chr8_1:81231675-81231694	None:intergenic	55.0%
	CATCGCCACACTTCCACCAA+GGG	+	chr8_1:81231681-81231700	Msa1218590:CDS	55.0%
	ATCGCCACACTTCCACCAAG+GGG	+	chr8_1:81231682-81231701	Msa1218590:CDS	55.0%
	TTACCCCCTTGGTGGAAGTG+TGG	-	chr8_1:81231689-81231708	None:intergenic	55.0%
	GCATCGCCACACTTCCACCA+AGG	+	chr8_1:81231680-81231699	Msa1218590:CDS	60.0%
	TCGCCACACTTCCACCAAGG+GGG	+	chr8_1:81231683-81231702	Msa1218590:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	81231603	81232086	81231603	ID=Msa1218590;Name=Msa1218590
chr8_1	mRNA	81231603	81232086	81231603	ID=Msa1218590-mRNA-1;Parent=Msa1218590;Name=Msa1218590-mRNA-1;_AED=0.34;_eAED=0.34;_QI=0\|1\|1\|1\|1\|1\|2\|172\|61
chr8_1	exon	81231603	81231704	81231603	ID=Msa1218590-mRNA-1:exon:28711;Parent=Msa1218590-mRNA-1
chr8_1	exon	81231831	81232086	81231831	ID=Msa1218590-mRNA-1:exon:28712;Parent=Msa1218590-mRNA-1
chr8_1	CDS	81231603	81231704	81231603	ID=Msa1218590-mRNA-1:cds;Parent=Msa1218590-mRNA-1
chr8_1	CDS	81231831	81231914	81231831	ID=Msa1218590-mRNA-1:cds;Parent=Msa1218590-mRNA-1
chr8_1	three_prime_UTR	81231915	81232086	81231915	ID=Msa1218590-mRNA-1:three_prime_utr;Parent=Msa1218590-mRNA-1

Gene Sequence	>Msa1218590 ATGGCGAAGTCGAAGAATCACACCGCTCACAACCAGTCTTACAAGGCTCACAAGAATGGCATCAAGAAGCCAAAGAGGCATCGCCACACTTCCACCAAGGGGATGGATCCAAAGTTTTTGAGGAACCAGAGGTATGCAAGGAAGCACAACAAGAAAAACGGTGAAATCGCCACTGAAGAAGAGTAA
Protein sequence	>Msa1218590 MAKSKNHTAHNQSYKAHKNGIKKPKRHRHTSTKGMDPKFLRNQRYARKHNKKNGEIATEEE*