Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0002230 | Msa1195190 | 0.801567 | 8.473890e-49 | -8.615850e-47 |
| Msa0002240 | Msa1195190 | 0.807452 | 5.019035e-50 | -8.615850e-47 |
| Msa0003890 | Msa1195190 | 0.814047 | 1.879328e-51 | -8.615850e-47 |
| Msa0006070 | Msa1195190 | 0.800614 | 1.326827e-48 | -8.615850e-47 |
| Msa0006110 | Msa1195190 | 0.838786 | 2.338206e-57 | -8.615850e-47 |
| Msa0011170 | Msa1195190 | 0.820767 | 5.764798e-53 | -8.615850e-47 |
| Msa0014710 | Msa1195190 | 0.826747 | 2.289499e-54 | -8.615850e-47 |
| Msa0015130 | Msa1195190 | 0.808688 | 2.739503e-50 | -8.615850e-47 |
| Msa0015390 | Msa1195190 | 0.809831 | 1.557480e-50 | -8.615850e-47 |
| Msa0015610 | Msa1195190 | 0.800763 | 1.237503e-48 | -8.615850e-47 |
Find 5 sgRNAs with CRISPR-Local
Find 126 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACGACACTTTGAAAAGAATG+TGG | 0.692687 | 8_1:+43003804 | Msa1195190:CDS |
| ATCACACGCTTATTTGTTGA+AGG | 0.441603 | 8_1:-43002202 | None:intergenic |
| CGTCTTAGCAGAGCTAGGTT+TGG | 0.434111 | 8_1:-43003755 | None:intergenic |
| GGATTCGTCTTAGCAGAGCT+AGG | 0.559567 | 8_1:-43003760 | None:intergenic |
| TCATCTACAGCTTCAATCTT+TGG | 0.233065 | 8_1:-43003781 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTCTTTCTTTCAATGTGTTC+AGG | - | chr8_1:43002363-43002382 | None:intergenic | 30.0% | |
| TTATAGAACAGAGTTCTCTT+TGG | - | chr8_1:43002464-43002483 | None:intergenic | 30.0% | |
| GTCTTACATAATGAGTTGTT+TGG | + | chr8_1:43003162-43003181 | Msa1195190:intron | 30.0% | |
| TTAATTCTGGTGAAGAGTAA+AGG | + | chr8_1:43003241-43003260 | Msa1195190:intron | 30.0% | |
| GTTTCTTGCAATTCCATAAA+AGG | - | chr8_1:43003376-43003395 | None:intergenic | 30.0% | |
| GCTGATAACAATTTGTATGA+AGG | - | chr8_1:43003398-43003417 | None:intergenic | 30.0% | |
| TTCTTTCTTTCAATGTGTTC+AGG | - | chr8_1:43002363-43002382 | None:intergenic | 30.0% | |
| TTATAGAACAGAGTTCTCTT+TGG | - | chr8_1:43002464-43002483 | None:intergenic | 30.0% | |
| GTCTTACATAATGAGTTGTT+TGG | + | chr8_1:43003162-43003181 | Msa1195190:intron | 30.0% | |
| TTAATTCTGGTGAAGAGTAA+AGG | + | chr8_1:43003241-43003260 | Msa1195190:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8_1 | gene | 43002160 | 43003834 | 43002160 | ID=Msa1195190;Name=Msa1195190 |
| chr8_1 | mRNA | 43002160 | 43003834 | 43002160 | ID=Msa1195190-mRNA-1;Parent=Msa1195190;Name=Msa1195190-mRNA-1;_AED=0.83;_eAED=1.00;_QI=0|0|0|0.66|0|0.66|3|0|70 |
| chr8_1 | exon | 43002160 | 43002219 | 43002160 | ID=Msa1195190-mRNA-1:exon:13892;Parent=Msa1195190-mRNA-1 |
| chr8_1 | exon | 43002267 | 43002272 | 43002267 | ID=Msa1195190-mRNA-1:exon:13893;Parent=Msa1195190-mRNA-1 |
| chr8_1 | exon | 43003688 | 43003834 | 43003688 | ID=Msa1195190-mRNA-1:exon:13894;Parent=Msa1195190-mRNA-1 |
| chr8_1 | CDS | 43002160 | 43002219 | 43002160 | ID=Msa1195190-mRNA-1:cds;Parent=Msa1195190-mRNA-1 |
| chr8_1 | CDS | 43002267 | 43002272 | 43002267 | ID=Msa1195190-mRNA-1:cds;Parent=Msa1195190-mRNA-1 |
| chr8_1 | CDS | 43003688 | 43003834 | 43003688 | ID=Msa1195190-mRNA-1:cds;Parent=Msa1195190-mRNA-1 |
| Gene Sequence |
>Msa1195190 ATGATAATCAAAGTACAAGCAGCAACAGCTTTAGTAAGATTACCTTCAACAAATAAGCGTATTTTTTATATCCCCAAGCAAATTTTAAAAAGAGTTCGGAAAGAAAAGTGCAAGAGAGATACACTCAAAAAGACCAAACCTAGCTCTGCTAAGACGAATCCAAAGATTGAAGCTGTAGATGAACGACACTTTGAAAAGAATGTGGTTGAATGA |
| Protein sequence |
>Msa1195190 MIIKVQAATALVRLPSTNKRIFYIPKQILKRVRKEKCKRDTLKKTKPSSAKTNPKIEAVDERHFEKNVVE* |
