AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene77663


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77663.t1 MTR_8g024180 93.103 87 6 0 1 87 65 151 2.42e-56 171
MS.gene77663.t1 MTR_7g118290 68.966 87 24 1 1 87 77 160 5.33e-38 125
MS.gene77663.t1 MTR_4g013200 48.529 68 35 0 20 87 61 128 1.81e-17 72.0
MS.gene77663.t1 MTR_7g118290 45.977 87 23 2 1 87 77 139 5.52e-17 71.2
MS.gene77663.t1 MTR_7g116795 39.130 69 42 0 17 85 72 140 1.46e-14 64.7
MS.gene77663.t1 MTR_7g118290 50.000 62 27 2 1 62 77 134 8.26e-12 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77663.t1 AT2G46820 75.714 70 17 0 18 87 86 155 6.15e-35 117
MS.gene77663.t1 AT2G46820 75.714 70 17 0 18 87 86 155 6.15e-35 117
MS.gene77663.t1 AT4G01150 36.471 85 54 0 1 85 59 143 1.41e-16 70.1
MS.gene77663.t1 AT1G52220 40.260 77 46 0 11 87 62 138 3.60e-16 68.9
MS.gene77663.t1 AT1G52220 40.260 77 45 1 11 87 62 137 2.11e-14 64.3

Find 17 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAAATTATGCATACCTTTC+TGG 0.079710 8.1:+76188248 None:intergenic
CCTGGAGTTCTTGAGTTAAT+AGG 0.246781 8.1:-76189179 MS.gene77663:CDS
GATAGACTTCCTTTGATTCC+TGG 0.345188 8.1:-76189197 MS.gene77663:CDS
GTTCTTGAGTTAATAGGAAT+TGG 0.346748 8.1:-76189173 MS.gene77663:CDS
TAGCTAGTGTATTTGCACTA+TGG 0.434144 8.1:-76189349 MS.gene77663:CDS
GATGTTATGGTAATGGCAAC+AGG 0.459632 8.1:-76189582 None:intergenic
TTTGCACTATGGAGCTCAGC+TGG 0.465802 8.1:-76189338 MS.gene77663:CDS
TGAAGATTCTTAACAAACTC+AGG 0.486308 8.1:+76189525 None:intergenic
TCAGTTGAGTCAACTTCTGT+TGG 0.503744 8.1:+76189552 None:intergenic
TAAATATGCAGTGGGTTCAT+TGG 0.505381 8.1:-76189375 MS.gene77663:CDS
CCTATTAACTCAAGAACTCC+AGG 0.507872 8.1:+76189179 None:intergenic
TTAATAGGAATTGGTTACAC+TGG 0.524822 8.1:-76189164 MS.gene77663:CDS
TAATAGGAATTGGTTACACT+GGG 0.540472 8.1:-76189163 MS.gene77663:CDS
TCAAGAACTCCAGGAATCAA+AGG 0.544788 8.1:+76189188 None:intergenic
TTTGATGATAAATATGCAGT+GGG 0.562064 8.1:-76189383 MS.gene77663:CDS
ATTTGATGATAAATATGCAG+TGG 0.570116 8.1:-76189384 MS.gene77663:CDS
AATAGGAATTGGTTACACTG+GGG 0.760636 8.1:-76189162 MS.gene77663:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAAATAACATAATTCT+AGG + chr8.1:76188408-76188427 None:intergenic 10.0%
!! ATAAAAATGTATTACAATAT+CGG + chr8.1:76189136-76189155 None:intergenic 10.0%
!! TATATGTATATATATATATG+AGG - chr8.1:76189466-76189485 MS.gene77663:intron 10.0%
!! TATTTCAAAAAAAAAATACA+AGG + chr8.1:76188769-76188788 None:intergenic 10.0%
!!! AATTATGTTATTTATTTTGT+TGG - chr8.1:76188410-76188429 MS.gene77663:intron 10.0%
!! TCAATAATAAACATTAAGAA+GGG + chr8.1:76188385-76188404 None:intergenic 15.0%
!!! TATTTTGTCATAAAATCAAA+TGG + chr8.1:76189313-76189332 None:intergenic 15.0%
!! AAATATAGAAGATGAATACT+AGG + chr8.1:76189525-76189544 None:intergenic 20.0%
!! CTTAGATTTAAATATATGAC+TGG - chr8.1:76188711-76188730 MS.gene77663:intron 20.0%
!! GTCAATAATAAACATTAAGA+AGG + chr8.1:76188386-76188405 None:intergenic 20.0%
!! TCTAATCTTATCAATTTGTA+AGG + chr8.1:76189176-76189195 None:intergenic 20.0%
!! TTACAAATTGATAAGATTAG+AGG - chr8.1:76189175-76189194 MS.gene77663:CDS 20.0%
!!! TTTTTTTCCAGTAATTTCTA+GGG - chr8.1:76189253-76189272 MS.gene77663:intron 20.0%
! TAGGTTGTTAGAAATTGTAA+TGG - chr8.1:76188348-76188367 MS.gene77663:intron 25.0%
!! ATTTGATGATAAATATGCAG+TGG - chr8.1:76188449-76188468 MS.gene77663:intron 25.0%
!! TATGAGGTATTATTCATCTT+TGG - chr8.1:76189482-76189501 MS.gene77663:intron 25.0%
!! TTTGATGATAAATATGCAGT+GGG - chr8.1:76188450-76188469 MS.gene77663:intron 25.0%
!!! AATATCGCGTATTTGATTTT+TGG - chr8.1:76188742-76188761 MS.gene77663:intron 25.0%
!!! GTTTTTTTCCAGTAATTTCT+AGG - chr8.1:76189252-76189271 MS.gene77663:intron 25.0%
!!! TTGTTTTTTAAAATGATCCC+CGG - chr8.1:76188806-76188825 MS.gene77663:intron 25.0%
AAGAAAACCCTAGAAATTAC+TGG + chr8.1:76189263-76189282 None:intergenic 30.0%
AAGCACAATAGTATTTGACA+TGG + chr8.1:76189048-76189067 None:intergenic 30.0%
AATATCGGACTCTATCTATA+AGG + chr8.1:76189121-76189140 None:intergenic 30.0%
AGCACAATAGTATTTGACAT+GGG + chr8.1:76189047-76189066 None:intergenic 30.0%
ATCAAATGGCTTTCTTAAGA+TGG + chr8.1:76189299-76189318 None:intergenic 30.0%
ATGTCAAATACTATTGTGCT+TGG - chr8.1:76189047-76189066 MS.gene77663:intron 30.0%
CAATTACTTTCACTTGTAAC+AGG - chr8.1:76188608-76188627 MS.gene77663:intron 30.0%
GCATCAAGTGAAATAATGTT+GGG + chr8.1:76188941-76188960 None:intergenic 30.0%
GCTTTCTTAAGATGGTATTT+AGG + chr8.1:76189291-76189310 None:intergenic 30.0%
GTTCTTGAGTTAATAGGAAT+TGG - chr8.1:76188660-76188679 MS.gene77663:intron 30.0%
TAATAGGAATTGGTTACACT+GGG - chr8.1:76188670-76188689 MS.gene77663:intron 30.0%
TGAAGATTCTTAACAAACTC+AGG + chr8.1:76188311-76188330 None:intergenic 30.0%
TGCATCAAGTGAAATAATGT+TGG + chr8.1:76188942-76188961 None:intergenic 30.0%
TTAATAGGAATTGGTTACAC+TGG - chr8.1:76188669-76188688 MS.gene77663:intron 30.0%
! ACTTGATGCATTGATATACA+TGG - chr8.1:76188952-76188971 MS.gene77663:intron 30.0%
! TATTTTGTTGGCATCATAGT+GGG - chr8.1:76188422-76188441 MS.gene77663:intron 30.0%
!! TTATTTTGTTGGCATCATAG+TGG - chr8.1:76188421-76188440 MS.gene77663:intron 30.0%
!!! TGTTTTTTAAAATGATCCCC+GGG - chr8.1:76188807-76188826 MS.gene77663:intron 30.0%
AATAGGAATTGGTTACACTG+GGG - chr8.1:76188671-76188690 MS.gene77663:intron 35.0%
ACAATAGTATTTGACATGGG+GGG + chr8.1:76189044-76189063 None:intergenic 35.0%
ATCAAATACGCGATATTTGC+AGG + chr8.1:76188739-76188758 None:intergenic 35.0%
ATTGACATATAGATAGGGGA+TGG - chr8.1:76189073-76189092 MS.gene77663:intron 35.0%
CAAGAAGCTGTATGATACAT+AGG - chr8.1:76188329-76188348 MS.gene77663:intron 35.0%
CACAATAGTATTTGACATGG+GGG + chr8.1:76189045-76189064 None:intergenic 35.0%
CCTGCATTGAATAGTGTATA+AGG + chr8.1:76188990-76189009 None:intergenic 35.0%
CCTTATACACTATTCAATGC+AGG - chr8.1:76188987-76189006 MS.gene77663:intron 35.0%
GCACAATAGTATTTGACATG+GGG + chr8.1:76189046-76189065 None:intergenic 35.0%
TAGCTAGTGTATTTGCACTA+TGG - chr8.1:76188484-76188503 MS.gene77663:intron 35.0%
TCAAATACGCGATATTTGCA+GGG + chr8.1:76188738-76188757 None:intergenic 35.0%
TTCTATATTTCACCTTGCAG+TGG - chr8.1:76189535-76189554 MS.gene77663:CDS 35.0%
! GCATTGATATACATGGTTGA+TGG - chr8.1:76188959-76188978 MS.gene77663:intron 35.0%
! GGTGCATTGACATATAGATA+GGG - chr8.1:76189068-76189087 MS.gene77663:intron 35.0%
! GTGCATTGACATATAGATAG+GGG - chr8.1:76189069-76189088 MS.gene77663:intron 35.0%
! TAAATATGCAGTGGGTTCAT+TGG - chr8.1:76188458-76188477 MS.gene77663:intron 35.0%
! TGGTGCATTGACATATAGAT+AGG - chr8.1:76189067-76189086 MS.gene77663:intron 35.0%
!! GATGGCCTTATAGAAGATAT+GGG - chr8.1:76189091-76189110 MS.gene77663:intron 35.0%
AATAGTATTTGACATGGGGG+GGG + chr8.1:76189042-76189061 None:intergenic 40.0%
ACACTATTCAATGCAGGACT+TGG - chr8.1:76188993-76189012 MS.gene77663:intron 40.0%
CAATAGTATTTGACATGGGG+GGG + chr8.1:76189043-76189062 None:intergenic 40.0%
CCTATTAACTCAAGAACTCC+AGG + chr8.1:76188657-76188676 None:intergenic 40.0%
CCTGGAGTTCTTGAGTTAAT+AGG - chr8.1:76188654-76188673 MS.gene77663:intron 40.0%
GATAGACTTCCTTTGATTCC+TGG - chr8.1:76188636-76188655 MS.gene77663:intron 40.0%
GCCATCCCATATCTTCTATA+AGG + chr8.1:76189099-76189118 None:intergenic 40.0%
GCCTTATAGAAGATATGGGA+TGG - chr8.1:76189095-76189114 MS.gene77663:intron 40.0%
GTCCATTTAAGCTGATAGGT+GGG - chr8.1:76188829-76188848 MS.gene77663:intron 40.0%
TCAAGAACTCCAGGAATCAA+AGG + chr8.1:76188648-76188667 None:intergenic 40.0%
TCAGTTGAGTCAACTTCTGT+TGG + chr8.1:76188284-76188303 None:intergenic 40.0%
!! GATATACATGGTTGATGGTC+AGG - chr8.1:76188964-76188983 MS.gene77663:intron 40.0%
!! GGATGGCCTTATAGAAGATA+TGG - chr8.1:76189090-76189109 MS.gene77663:intron 40.0%
ATAGTATTTGACATGGGGGG+GGG + chr8.1:76189041-76189060 None:intergenic 45.0%
CCTATCAGCTTAAATGGACC+CGG + chr8.1:76188828-76188847 None:intergenic 45.0%
CTATCAGCTTAAATGGACCC+GGG + chr8.1:76188827-76188846 None:intergenic 45.0%
CTCCCACCTATCAGCTTAAA+TGG + chr8.1:76188834-76188853 None:intergenic 45.0%
CTTGTATGCAAACCACTGCA+AGG + chr8.1:76189550-76189569 None:intergenic 45.0%
GGTCCATTTAAGCTGATAGG+TGG - chr8.1:76188828-76188847 MS.gene77663:intron 45.0%
TATCAGCTTAAATGGACCCG+GGG + chr8.1:76188826-76188845 None:intergenic 45.0%
!!! GAGCTCAGCTGGTTTTATCT+CGG - chr8.1:76188506-76188525 MS.gene77663:intron 45.0%
CCGGGTCCATTTAAGCTGAT+AGG - chr8.1:76188825-76188844 MS.gene77663:intron 50.0%
TAGTATTTGACATGGGGGGG+GGG + chr8.1:76189040-76189059 None:intergenic 50.0%
TTTGCACTATGGAGCTCAGC+TGG - chr8.1:76188495-76188514 MS.gene77663:intron 50.0%
AGTATTTGACATGGGGGGGG+GGG + chr8.1:76189039-76189058 None:intergenic 55.0%
GTATTTGACATGGGGGGGGG+GGG + chr8.1:76189038-76189057 None:intergenic 60.0%
TATTTGACATGGGGGGGGGG+GGG + chr8.1:76189037-76189056 None:intergenic 60.0%
ATTTGACATGGGGGGGGGGG+GGG + chr8.1:76189036-76189055 None:intergenic 65.0%
TTTGACATGGGGGGGGGGGG+GGG + chr8.1:76189035-76189054 None:intergenic 70.0%
TTGACATGGGGGGGGGGGGG+GGG + chr8.1:76189034-76189053 None:intergenic 75.0%
! TGACATGGGGGGGGGGGGGG+GGG + chr8.1:76189033-76189052 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 76188263 76189592 76188263 ID=MS.gene77663
chr8.1 mRNA 76188263 76189592 76188263 ID=MS.gene77663.t1;Parent=MS.gene77663
chr8.1 exon 76189518 76189592 76189518 ID=MS.gene77663.t1.exon1;Parent=MS.gene77663.t1
chr8.1 CDS 76189518 76189592 76189518 ID=cds.MS.gene77663.t1;Parent=MS.gene77663.t1
chr8.1 exon 76189328 76189414 76189328 ID=MS.gene77663.t1.exon2;Parent=MS.gene77663.t1
chr8.1 CDS 76189328 76189414 76189328 ID=cds.MS.gene77663.t1;Parent=MS.gene77663.t1
chr8.1 exon 76189163 76189225 76189163 ID=MS.gene77663.t1.exon3;Parent=MS.gene77663.t1
chr8.1 CDS 76189163 76189225 76189163 ID=cds.MS.gene77663.t1;Parent=MS.gene77663.t1
chr8.1 exon 76188263 76188300 76188263 ID=MS.gene77663.t1.exon4;Parent=MS.gene77663.t1
chr8.1 CDS 76188263 76188300 76188263 ID=cds.MS.gene77663.t1;Parent=MS.gene77663.t1
Gene Sequence

>MS.gene77663

ATGGCAACAGGAGATGCACCAACAGAAGTTGACTCAACTGAGCTACCTGAGTTTGTTAAGAATCTTCAAGAAGCTGTATGATACATAGGTTGTTAGAAATTGTAATGGAGTTTCTTTTTCCCTTCTTAATGTTTATTATTGACCTAGAATTATGTTATTTATTTTGTTGGCATCATAGTGGGACAAATTTGATGATAAATATGCAGTGGGTTCATTGGTTGTAGCTAGTGTATTTGCACTATGGAGCTCAGCTGGTTTTATCTCGGTATTAAGTTATTTAAATGCTAATTTTATTTGCATTATTTGATTATCAATTTATTAGATACAGATGATAAATGTCATAAGCAATTACTTTCACTTGTAACAGGCAATTGATAGACTTCCTTTGATTCCTGGAGTTCTTGAGTTAATAGGAATTGGTTACACTGGGGTAAGTCAAATAAATACACTTAGATTTAAATATATGACTGGTCCCTGCAAATATCGCGTATTTGATTTTTGGTCCTTGTATTTTTTTTTTGAAATACATCTTTGCAAGAAAAATTGTTTTTTAAAATGATCCCCGGGTCCATTTAAGCTGATAGGTGGGAGTCTGATGCTTACGTTACACATGCTAAAAAAAATAAAATCTGAAACTTAATTTAAAAAAAAAATATAAATAGATTTTTTCATATGCCCAACATTATTTCACTTGATGCATTGATATACATGGTTGATGGTCAGGCCTTATACACTATTCAATGCAGGACTTGGATTTTTGTTTTACACCCCCCCCCCCCCCCCCATGTCAAATACTATTGTGCTTGGTGCATTGACATATAGATAGGGGATGGCCTTATAGAAGATATGGGATGGCCTTATAGATAGAGTCCGATATTGTAATACATTTTTATATTTAGACATAACTCATCCTTACAAATTGATAAGATTAGAGGTGTCACTCTTTATAAACTCATCTCAAGCATTATATTTATTCAATGCGACACTTAGTTTTTTTCCAGTAATTTCTAGGGTTTTCTTTTAATCCTAAATACCATCTTAAGAAAGCCATTTGATTTTATGACAAAATACATATATACTAGCAAAAACAATATATATATATATATATGTATATATATCTATATATATATATGTATATATATATCTATATATATGTATATATATATATATATATATATATATATATATGTGTATATATATATATATATGTATATATATATATGAGGTATTATTCATCTTTGGATTCAACTATAAGAAAACCTAGTATTCATCTTCTATATTTCACCTTGCAGTGGTTTGCATACAAGAACGTAGTTTTCAAGCCAGAAAG

Protein sequence

>MS.gene77663.t1

MATGDAPTEVDSTELPEFVKNLQEAWDKFDDKYAVGSLVVASVFALWSSAGFISAIDRLPLIPGVLELIGIGYTGWFAYKNVVFKPE