AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene32766


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32766.t1 MTR_3g104980 95.261 211 10 0 1 211 1 211 3.08e-143 398
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene32766.t1 AT5G21430 55.738 183 77 3 32 210 35 217 1.83e-62 194
MS.gene32766.t1 AT5G21430 55.191 183 77 4 32 210 35 216 5.39e-60 187

Find 50 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTAAAGAAGATGATTCTTT+TGG 0.261740 3.1:-82911512 None:intergenic
AGACATACTTAACAAGAAAT+TGG 0.280104 3.1:+82911944 MS.gene32766:CDS
ATTGGAGAGTCAAGGTTTAG+AGG 0.312619 3.1:+82911920 MS.gene32766:CDS
GCCATTTGAGGTTGATGTTT+CGG 0.315689 3.1:+82912163 MS.gene32766:CDS
TAATAACAAGGTTCGAGAAT+TGG 0.322385 3.1:+82911902 MS.gene32766:CDS
CTAGCATTTGTGTTATCAAT+TGG 0.334884 3.1:+82912402 MS.gene32766:CDS
ATTACAGATGCAGATCATTA+TGG 0.366825 3.1:+82911662 MS.gene32766:CDS
TGTGTCTGTGTTATCTTCTC+TGG 0.408544 3.1:-82912132 None:intergenic
ACCTTGTTATTATAAGCAAT+TGG 0.411448 3.1:-82911891 None:intergenic
CACATACGTTTGGCCATTTG+AGG 0.421335 3.1:+82912151 MS.gene32766:CDS
TCATACATTCTTCTTTCATC+CGG 0.438615 3.1:-82912098 None:intergenic
ATGTTTCTGACCATGTCATA+TGG 0.442154 3.1:-82911710 None:intergenic
TTGCTTGGAGTGTTGTTACT+TGG 0.444787 3.1:-82911365 None:intergenic
GTTGAGAATCCTAGCTTGCT+TGG 0.458033 3.1:-82911380 None:intergenic
CTGGTTGGTCCTACATCCTC+AGG 0.458680 3.1:-82912345 None:intergenic
ACCGAAACATCAACCTCAAA+TGG 0.480733 3.1:-82912164 None:intergenic
GCAGATCATTATGGTAGACT+TGG 0.523390 3.1:+82911671 MS.gene32766:CDS
GCAAATTGTAGTGGTGGTGG+TGG 0.527140 3.1:-82911339 None:intergenic
AGCTCTAGTGAAAACTCTGC+AGG 0.533335 3.1:+82911452 MS.gene32766:CDS
TAGAAAGTATCCAACCAGTC+TGG 0.536223 3.1:-82912364 None:intergenic
CTGGTTGGATACTTTCTAGC+GGG 0.536918 3.1:+82912369 MS.gene32766:CDS
ATAACACAGACACATACGTT+TGG 0.539673 3.1:+82912141 MS.gene32766:CDS
ATGAAAGAAGAATGTATGAC+TGG 0.543817 3.1:+82912102 MS.gene32766:CDS
GATGTTTCGGTACTTCAAGA+AGG 0.552371 3.1:+82912176 MS.gene32766:CDS
ATGTCATATGGACATCCTCT+TGG 0.556234 3.1:-82911698 None:intergenic
AGTGGTGGTGGTGGAGGACA+AGG 0.559487 3.1:-82911330 None:intergenic
AATTGTAGTGGTGGTGGTGG+AGG 0.559668 3.1:-82911336 None:intergenic
TTGGATACTTTCTAGCGGGA+TGG 0.565729 3.1:+82912373 MS.gene32766:CDS
GTAATTAGTACCTGTGGTGG+AGG 0.566183 3.1:-82912203 None:intergenic
GTTCGAGAATTGGAGAGTCA+AGG 0.571210 3.1:+82911912 MS.gene32766:CDS
GTAGGACCAACCAGACTGGT+TGG 0.579118 3.1:+82912354 MS.gene32766:CDS
TGAAAACTCTGCAGGTTCAA+CGG 0.580415 3.1:+82911460 MS.gene32766:CDS
CAAGTAATTAGTACCTGTGG+TGG 0.590198 3.1:-82912206 None:intergenic
GGATGTAGGACCAACCAGAC+TGG 0.590754 3.1:+82912350 MS.gene32766:CDS
TGGCAGGAACCTGAGGATGT+AGG 0.592315 3.1:+82912336 MS.gene32766:intron
AACAACACTCCAAGCAAGCT+AGG 0.601547 3.1:+82911371 MS.gene32766:CDS
ATACACTATCATGTCATCGC+CGG 0.604771 3.1:+82912079 MS.gene32766:CDS
GCCAATTGCTTATAATAACA+AGG 0.606196 3.1:+82911890 MS.gene32766:CDS
AATGCAAATTGTAGTGGTGG+TGG 0.612182 3.1:-82911342 None:intergenic
AGGTTCAACGGAGGTTGAGT+CGG 0.620899 3.1:+82911472 MS.gene32766:CDS
AAGTATCCAACCAGTCTGGT+TGG 0.621927 3.1:-82912360 None:intergenic
AGAAGGAGATCCTCCACCAC+AGG 0.633320 3.1:+82912193 MS.gene32766:CDS
ACTTGGAATGCAAATTGTAG+TGG 0.640752 3.1:-82911348 None:intergenic
GGTAGACTTGGCTTACCAAG+AGG 0.643717 3.1:+82911683 MS.gene32766:CDS
TGGAAAACATACGTATGCCA+CGG 0.651444 3.1:-82911562 None:intergenic
TGGAATGCAAATTGTAGTGG+TGG 0.664067 3.1:-82911345 None:intergenic
AAGAGGATGTCCATATGACA+TGG 0.675165 3.1:+82911700 MS.gene32766:CDS
ACTGGTTGGATACTTTCTAG+CGG 0.691428 3.1:+82912368 MS.gene32766:CDS
AACGTCGAAAGAGCTCTCCG+TGG 0.728158 3.1:+82911545 MS.gene32766:CDS
AAACTCTGCAGGTTCAACGG+AGG 0.732304 3.1:+82911463 MS.gene32766:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATAATAACAACAACATTA+TGG - chr3.1:82911585-82911604 None:intergenic 15.0%
!! TTGAAATGATCCATTTAAAA+CGG + chr3.1:82912298-82912317 MS.gene32766:intron 20.0%
!!! ATTAAAGAAGATGATTCTTT+TGG - chr3.1:82911515-82911534 None:intergenic 20.0%
! AGACATACTTAACAAGAAAT+TGG + chr3.1:82911944-82911963 MS.gene32766:CDS 25.0%
! GAATTGAATTGATGAAGATA+TGG + chr3.1:82911625-82911644 MS.gene32766:intron 25.0%
! GTATGATTCCTATACAATTA+AGG - chr3.1:82912063-82912082 None:intergenic 25.0%
! TCGGATTTAATTCATATGAA+AGG + chr3.1:82911739-82911758 MS.gene32766:intron 25.0%
! TTGGTTAACCTTAATTGTAT+AGG + chr3.1:82912052-82912071 MS.gene32766:intron 25.0%
!! TGGTCAGAAACATTTTATAT+CGG + chr3.1:82911720-82911739 MS.gene32766:intron 25.0%
!! TTTCTATTTCTCAACTTCTT+AGG + chr3.1:82912229-82912248 MS.gene32766:intron 25.0%
!!! ACCTTGTTATTATAAGCAAT+TGG - chr3.1:82911894-82911913 None:intergenic 25.0%
!!! ATTGGAAATACCGTTTTAAA+TGG - chr3.1:82912311-82912330 None:intergenic 25.0%
AAACAAGTAATTAGTACCTG+TGG - chr3.1:82912212-82912231 None:intergenic 30.0%
ATGAAAGAAGAATGTATGAC+TGG + chr3.1:82912102-82912121 MS.gene32766:CDS 30.0%
ATTACAGATGCAGATCATTA+TGG + chr3.1:82911662-82911681 MS.gene32766:CDS 30.0%
CTAGCATTTGTGTTATCAAT+TGG + chr3.1:82912402-82912421 MS.gene32766:CDS 30.0%
GCCAATTGCTTATAATAACA+AGG + chr3.1:82911890-82911909 MS.gene32766:CDS 30.0%
TAATAACAAGGTTCGAGAAT+TGG + chr3.1:82911902-82911921 MS.gene32766:CDS 30.0%
TCATACATTCTTCTTTCATC+CGG - chr3.1:82912101-82912120 None:intergenic 30.0%
TGTGTTTGCATCGTTATTAT+AGG + chr3.1:82912008-82912027 MS.gene32766:intron 30.0%
! AACGGTATTTCCAATTTTCA+TGG + chr3.1:82912316-82912335 MS.gene32766:intron 30.0%
!! TGATTCTTTTGGAAGTTCAA+TGG - chr3.1:82911504-82911523 None:intergenic 30.0%
!!! TTTTTTCTGTAAAACAAGCG+CGG + chr3.1:82911414-82911433 MS.gene32766:CDS 30.0%
ACTTGGAATGCAAATTGTAG+TGG - chr3.1:82911351-82911370 None:intergenic 35.0%
ATAACACAGACACATACGTT+TGG + chr3.1:82912141-82912160 MS.gene32766:CDS 35.0%
ATGTCACTCTACACTTTAAC+AGG + chr3.1:82911866-82911885 MS.gene32766:intron 35.0%
TATTATAGGTGAGACTTGAG+AGG + chr3.1:82912022-82912041 MS.gene32766:intron 35.0%
! ATGTTTCTGACCATGTCATA+TGG - chr3.1:82911713-82911732 None:intergenic 35.0%
! CTATTTCTCAACTTCTTAGG+AGG + chr3.1:82912232-82912251 MS.gene32766:intron 35.0%
! GTATTTCCAATTTTCATGGC+AGG + chr3.1:82912320-82912339 MS.gene32766:intron 35.0%
AAGAGGATGTCCATATGACA+TGG + chr3.1:82911700-82911719 MS.gene32766:CDS 40.0%
AATGCAAATTGTAGTGGTGG+TGG - chr3.1:82911345-82911364 None:intergenic 40.0%
ACCGAAACATCAACCTCAAA+TGG - chr3.1:82912167-82912186 None:intergenic 40.0%
ACTGGTTGGATACTTTCTAG+CGG + chr3.1:82912368-82912387 MS.gene32766:CDS 40.0%
ATACACTATCATGTCATCGC+CGG + chr3.1:82912079-82912098 MS.gene32766:CDS 40.0%
ATGTCATATGGACATCCTCT+TGG - chr3.1:82911701-82911720 None:intergenic 40.0%
ATTGGAGAGTCAAGGTTTAG+AGG + chr3.1:82911920-82911939 MS.gene32766:CDS 40.0%
CAAGTAATTAGTACCTGTGG+TGG - chr3.1:82912209-82912228 None:intergenic 40.0%
GATGTTTCGGTACTTCAAGA+AGG + chr3.1:82912176-82912195 MS.gene32766:CDS 40.0%
GCAGATCATTATGGTAGACT+TGG + chr3.1:82911671-82911690 MS.gene32766:CDS 40.0%
TGAAAACTCTGCAGGTTCAA+CGG + chr3.1:82911460-82911479 MS.gene32766:CDS 40.0%
TGGAAAACATACGTATGCCA+CGG - chr3.1:82911565-82911584 None:intergenic 40.0%
TGGAATGCAAATTGTAGTGG+TGG - chr3.1:82911348-82911367 None:intergenic 40.0%
TGTGTCTGTGTTATCTTCTC+TGG - chr3.1:82912135-82912154 None:intergenic 40.0%
!! AACTTCTTAGGAGGTGTTTG+AGG + chr3.1:82912241-82912260 MS.gene32766:intron 40.0%
!! GCCATTTGAGGTTGATGTTT+CGG + chr3.1:82912163-82912182 MS.gene32766:CDS 40.0%
!! TAGAAAGTATCCAACCAGTC+TGG - chr3.1:82912367-82912386 None:intergenic 40.0%
!! TTGCTTGGAGTGTTGTTACT+TGG - chr3.1:82911368-82911387 None:intergenic 40.0%
AACAACACTCCAAGCAAGCT+AGG + chr3.1:82911371-82911390 MS.gene32766:CDS 45.0%
AAGTATCCAACCAGTCTGGT+TGG - chr3.1:82912363-82912382 None:intergenic 45.0%
AGACTTGAGAGGTGGTGATT+TGG + chr3.1:82912033-82912052 MS.gene32766:intron 45.0%
CACATACGTTTGGCCATTTG+AGG + chr3.1:82912151-82912170 MS.gene32766:CDS 45.0%
CAGGTTCCTGCCATGAAAAT+TGG - chr3.1:82912329-82912348 None:intergenic 45.0%
GTAATTAGTACCTGTGGTGG+AGG - chr3.1:82912206-82912225 None:intergenic 45.0%
GTTCGAGAATTGGAGAGTCA+AGG + chr3.1:82911912-82911931 MS.gene32766:CDS 45.0%
TATAGGTGAGACTTGAGAGG+TGG + chr3.1:82912025-82912044 MS.gene32766:intron 45.0%
! AGCTCTAGTGAAAACTCTGC+AGG + chr3.1:82911452-82911471 MS.gene32766:CDS 45.0%
! ATTTTCATGGCAGGAACCTG+AGG + chr3.1:82912329-82912348 MS.gene32766:intron 45.0%
! CTGGTTGGATACTTTCTAGC+GGG + chr3.1:82912369-82912388 MS.gene32766:CDS 45.0%
! TTGGATACTTTCTAGCGGGA+TGG + chr3.1:82912373-82912392 MS.gene32766:CDS 45.0%
!! GTTGAGAATCCTAGCTTGCT+TGG - chr3.1:82911383-82911402 None:intergenic 45.0%
!! TTCTTAGGAGGTGTTTGAGG+TGG + chr3.1:82912244-82912263 MS.gene32766:intron 45.0%
AAACTCTGCAGGTTCAACGG+AGG + chr3.1:82911463-82911482 MS.gene32766:CDS 50.0%
AGGTTCAACGGAGGTTGAGT+CGG + chr3.1:82911472-82911491 MS.gene32766:CDS 50.0%
GGTAGACTTGGCTTACCAAG+AGG + chr3.1:82911683-82911702 MS.gene32766:CDS 50.0%
!! AATTGTAGTGGTGGTGGTGG+AGG - chr3.1:82911339-82911358 None:intergenic 50.0%
!! GCAAATTGTAGTGGTGGTGG+TGG - chr3.1:82911342-82911361 None:intergenic 50.0%
AACGTCGAAAGAGCTCTCCG+TGG + chr3.1:82911545-82911564 MS.gene32766:CDS 55.0%
AGAAGGAGATCCTCCACCAC+AGG + chr3.1:82912193-82912212 MS.gene32766:CDS 55.0%
GGATGTAGGACCAACCAGAC+TGG + chr3.1:82912350-82912369 MS.gene32766:CDS 55.0%
GTAGGACCAACCAGACTGGT+TGG + chr3.1:82912354-82912373 MS.gene32766:CDS 55.0%
TGGCAGGAACCTGAGGATGT+AGG + chr3.1:82912336-82912355 MS.gene32766:intron 55.0%
! CTGGTTGGTCCTACATCCTC+AGG - chr3.1:82912348-82912367 None:intergenic 55.0%
!! AGTGGTGGTGGTGGAGGACA+AGG - chr3.1:82911333-82911352 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 82911326 82912437 82911326 ID=MS.gene32766
chr3.1 mRNA 82911326 82912437 82911326 ID=MS.gene32766.t1;Parent=MS.gene32766
chr3.1 exon 82911326 82911572 82911326 ID=MS.gene32766.t1.exon1;Parent=MS.gene32766.t1
chr3.1 CDS 82911326 82911572 82911326 ID=cds.MS.gene32766.t1;Parent=MS.gene32766.t1
chr3.1 exon 82911660 82911721 82911660 ID=MS.gene32766.t1.exon2;Parent=MS.gene32766.t1
chr3.1 CDS 82911660 82911721 82911660 ID=cds.MS.gene32766.t1;Parent=MS.gene32766.t1
chr3.1 exon 82911888 82911977 82911888 ID=MS.gene32766.t1.exon3;Parent=MS.gene32766.t1
chr3.1 CDS 82911888 82911977 82911888 ID=cds.MS.gene32766.t1;Parent=MS.gene32766.t1
chr3.1 exon 82912074 82912214 82912074 ID=MS.gene32766.t1.exon4;Parent=MS.gene32766.t1
chr3.1 CDS 82912074 82912214 82912074 ID=cds.MS.gene32766.t1;Parent=MS.gene32766.t1
chr3.1 exon 82912342 82912437 82912342 ID=MS.gene32766.t1.exon5;Parent=MS.gene32766.t1
chr3.1 CDS 82912342 82912437 82912342 ID=cds.MS.gene32766.t1;Parent=MS.gene32766.t1
Gene Sequence

>MS.gene32766

ATGGCCTTGTCCTCCACCACCACCACTACAATTTGCATTCCAAGTAACAACACTCCAAGCAAGCTAGGATTCTCAACTAATAACATTGTTTTTTCTGTAAAACAAGCGCGGAAACTATGTATCAGAAGCTCTAGTGAAAACTCTGCAGGTTCAACGGAGGTTGAGTCGGAGAGTTCCATTGAACTTCCAAAAGAATCATCTTCTTTAATTTCTGTTCTCAACGTCGAAAGAGCTCTCCGTGGCATACGTATGTTTTCCATAATGTTGTTGTTATTATATGCTTAAAATTGCATTGCATTGAATTGAATTGATGAAGATATGGTTTAGAATGCAGCTATTACAGATGCAGATCATTATGGTAGACTTGGCTTACCAAGAGGATGTCCATATGACATGGTCAGAAACATTTTATATCGGATTTAATTCATATGAAAGGATTTTTATACAGTCAATCAATCATATTCGTCAAACATGAAAATCATTTGACTTCTAAAGTCACGCATGTATGATTGATGTTAATTCAACTTATCTTTTTGATCTATGTCACTCTACACTTTAACAGGTGCCAATTGCTTATAATAACAAGGTTCGAGAATTGGAGAGTCAAGGTTTAGAGGAAGACATACTTAACAAGAAATTGGAACTTCTCAGAGTTAGTATATATGCATGATTTGAATTGTTGTGTGTTTGCATCGTTATTATAGGTGAGACTTGAGAGGTGGTGATTTGGTTAACCTTAATTGTATAGGAATCATACACTATCATGTCATCGCCGGATGAAAGAAGAATGTATGACTGGAGCTTGTCCAGAGAAGATAACACAGACACATACGTTTGGCCATTTGAGGTTGATGTTTCGGTACTTCAAGAAGGAGATCCTCCACCACAGGTACTAATTACTTGTTTCTATTTCTCAACTTCTTAGGAGGTGTTTGAGGTGGAGAGAATTTCTCAAATATGATAATGAATTTGTTGAAATGATCCATTTAAAACGGTATTTCCAATTTTCATGGCAGGAACCTGAGGATGTAGGACCAACCAGACTGGTTGGATACTTTCTAGCGGGATGGATACTGCTAGCATTTGTGTTATCAATTGGACTAAACTTATAG

Protein sequence

>MS.gene32766.t1

MALSSTTTTTICIPSNNTPSKLGFSTNNIVFSVKQARKLCIRSSSENSAGSTEVESESSIELPKESSSLISVLNVERALRGIPITDADHYGRLGLPRGCPYDMVPIAYNNKVRELESQGLEEDILNKKLELLRESYTIMSSPDERRMYDWSLSREDNTDTYVWPFEVDVSVLQEGDPPPQEPEDVGPTRLVGYFLAGWILLAFVLSIGLNL