AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene82473

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82473.t1 MTR_5g025270 97.079 582 15 2 1 581 1 581 0.0 1154
MS.gene82473.t1 MTR_5g025230 82.646 582 96 4 1 581 1 578 0.0 985
MS.gene82473.t1 MTR_5g025250 81.959 582 101 3 1 581 1 579 0.0 976
MS.gene82473.t1 MTR_2g070460 36.301 584 335 13 24 577 32 608 2.98e-124 379
MS.gene82473.t1 MTR_8g037315 38.911 514 284 9 84 577 33 536 1.83e-113 348
MS.gene82473.t1 MTR_8g037275 37.475 499 283 9 100 577 81 571 5.95e-106 330
MS.gene82473.t1 MTR_8g037290 37.275 499 284 9 100 577 81 571 1.07e-104 327
MS.gene82473.t1 MTR_8g037310 36.434 516 299 10 83 577 64 571 1.79e-103 324
MS.gene82473.t1 MTR_3g110195 28.772 570 329 20 49 570 38 578 4.70e-57 201
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82473.t1 AT4G19170 68.214 560 150 6 29 578 54 595 0.0 791
MS.gene82473.t1 AT3G63520 40.077 519 281 8 83 580 28 537 1.71e-122 372
MS.gene82473.t1 AT1G30100 41.020 490 277 9 92 575 102 585 2.61e-121 370
MS.gene82473.t1 AT3G14440 40.323 496 281 10 92 580 112 599 6.53e-118 362
MS.gene82473.t1 AT4G18350 36.332 567 327 10 29 576 29 580 5.93e-116 357
MS.gene82473.t1 AT1G78390 38.259 494 285 11 96 581 176 657 4.88e-111 346
MS.gene82473.t1 AT3G24220 35.000 580 335 14 33 577 2 574 2.87e-103 323
MS.gene82473.t1 AT4G32810 24.459 601 352 28 31 570 8 567 2.35e-23 104
MS.gene82473.t1 AT2G44990 24.868 567 301 28 112 572 75 622 2.31e-11 67.0
MS.gene82473.t1 AT2G44990 24.868 567 301 28 112 572 71 618 2.43e-11 67.0

Find 144 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTATGGGTGGTGGTGGTGG+TGG 0.196963 5.2:-15961962 None:intergenic
AATCCTCTTGACATGATTTC+TGG 0.197088 5.2:+15962834 MS.gene82473:CDS
ATTTGCATCAAAACGGAAAT+AGG 0.215073 5.2:-15962700 None:intergenic
CTTGTTATTATGATGGGTTT+TGG 0.244030 5.2:-15961802 None:intergenic
AATCCAATGCCGAAGATTTC+AGG 0.254183 5.2:+15963221 MS.gene82473:CDS
ACACAATGTGAAATCATTAA+AGG 0.257197 5.2:+15962144 MS.gene82473:CDS
TTTGATGGATGAGATGTTAA+AGG 0.272209 5.2:-15961854 None:intergenic
GTGAAGATAAATATTAAAAC+AGG 0.290009 5.2:+15963095 MS.gene82473:CDS
CTGGACTTCGGGGTGATAAA+TGG 0.292154 5.2:+15963152 MS.gene82473:CDS
GGTGATAAATGGTGATTATA+TGG 0.293631 5.2:+15963163 MS.gene82473:CDS
AATCTTGACGGCCGATTGTT+TGG 0.307017 5.2:-15962575 None:intergenic
TGTTTGGGATTGTGATGTTT+GGG 0.324573 5.2:-15961890 None:intergenic
ACCGCAGCACGTGTCATATC+TGG 0.342484 5.2:+15962426 MS.gene82473:CDS
CAGCACGAAACCTGGACTTC+GGG 0.349258 5.2:+15963141 MS.gene82473:CDS
TCCAGGTTTCGTGCTGATAA+TGG 0.352248 5.2:-15963134 None:intergenic
GAGGTTATGGGTGGTGGTGG+TGG 0.353426 5.2:-15961965 None:intergenic
AACACAAGTTTGGCTTTGTT+TGG 0.358095 5.2:+15962483 MS.gene82473:CDS
GTGTTTGGGATTGTGATGTT+TGG 0.362352 5.2:-15961891 None:intergenic
TTGCTTACTTGTTATTATGA+TGG 0.362595 5.2:-15961809 None:intergenic
CGTGGAAGGAACTGAGGGTT+TGG 0.369571 5.2:-15962210 None:intergenic
AGGTTTGTTTATGCGGCAAT+TGG 0.386277 5.2:+15963197 MS.gene82473:CDS
GTGATAAATGGTGATTATAT+GGG 0.388122 5.2:+15963164 MS.gene82473:CDS
TTTGAGGTTATGGGTGGTGG+TGG 0.388850 5.2:-15961968 None:intergenic
TGGTTTAGCCAACACAAGTT+TGG 0.393154 5.2:+15962473 MS.gene82473:CDS
TTGAAACCAGAAAACACGTT+TGG 0.405859 5.2:-15962369 None:intergenic
TTGATGGATGAGATGTTAAA+GGG 0.406557 5.2:-15961853 None:intergenic
GCTAAACCAATGCCGTTAGA+AGG 0.408884 5.2:-15962459 None:intergenic
GGTGTAAGCTTGATTTCATA+AGG 0.411021 5.2:-15962540 None:intergenic
ATTATATGGGGAAGAAGAAT+AGG 0.419503 5.2:+15963177 MS.gene82473:CDS
ACCATTATCAGCACGAAACC+TGG 0.425051 5.2:+15963133 MS.gene82473:CDS
GAAGTTGGATGTAGATTGTA+TGG 0.425213 5.2:+15963275 MS.gene82473:CDS
TGCTTACTTGTTATTATGAT+GGG 0.425997 5.2:-15961808 None:intergenic
GGGAGTGATGATGGTGTTTG+TGG 0.434132 5.2:-15961994 None:intergenic
CCTAAAATTGACGGCGACAC+TGG 0.451020 5.2:+15962633 MS.gene82473:CDS
AAACTTCCACCGTCACTTAA+CGG 0.453134 5.2:+15962168 MS.gene82473:CDS
TGTTAAACATAACGATGTTC+CGG 0.453727 5.2:+15962725 MS.gene82473:CDS
AAACCATACGGCACCCTCCT+TGG 0.455270 5.2:-15963467 None:intergenic
TGAAACCAGAAAACACGTTT+GGG 0.456713 5.2:-15962368 None:intergenic
ATTTCTGGTGGTTCTCCAGT+AGG 0.459625 5.2:+15962849 MS.gene82473:CDS
TTGTATGGAGAGGATTGTTA+TGG 0.460237 5.2:+15963290 MS.gene82473:CDS
CCGATCCATCAAAAGTTTCA+AGG 0.463927 5.2:+15962874 MS.gene82473:CDS
ACTCCTGAAATCTTCGGCAT+TGG 0.467844 5.2:-15963224 None:intergenic
TGCTCAATGGAGAGAACATT+CGG 0.468229 5.2:-15963029 None:intergenic
TAACACCATTTGCATCAAAA+CGG 0.472715 5.2:-15962707 None:intergenic
CGCGATCAATGCGTGGGATG+GGG 0.473648 5.2:+15962974 MS.gene82473:CDS
TACAATCCTTCTAACGGCAT+TGG 0.476143 5.2:+15962453 MS.gene82473:CDS
ACGCGATCAATGCGTGGGAT+GGG 0.477959 5.2:+15962973 MS.gene82473:CDS
CATTTATCACCCCGAAGTCC+AGG 0.479778 5.2:-15963151 None:intergenic
GTGACGAGTCGAAGATGAAA+TGG 0.483688 5.2:+15962922 MS.gene82473:CDS
TTATGTGCATGATGAGAAGA+AGG 0.484903 5.2:+15963376 MS.gene82473:CDS
GTTTGCCGTTAAAATCTTGA+CGG 0.485908 5.2:-15962587 None:intergenic
CACGCGATCAATGCGTGGGA+TGG 0.488713 5.2:+15962972 MS.gene82473:CDS
CCTCTTGACATGATTTCTGG+TGG 0.488783 5.2:+15962837 MS.gene82473:CDS
GTGGAAGGAACTGAGGGTTT+GGG 0.489186 5.2:-15962209 None:intergenic
ATGAAATGGTTCGATGTGCC+AGG 0.489596 5.2:+15962936 MS.gene82473:CDS
ATTGTTTGGATATCACCGTT+TGG 0.495988 5.2:-15962561 None:intergenic
AACGGTGATATCCAAACAAT+CGG 0.496479 5.2:+15962564 MS.gene82473:CDS
GAAGAATAGGTTTGTTTATG+CGG 0.496498 5.2:+15963190 MS.gene82473:CDS
AACGGCGCTTACATAAGAAA+TGG 0.496894 5.2:+15962186 MS.gene82473:CDS
AAGGGAGGGATAGAAGTTGT+TGG 0.498248 5.2:-15961835 None:intergenic
AAAACACGTTTGGGATAAGT+TGG 0.498794 5.2:-15962359 None:intergenic
TTCACCACTCCTGAAATCTT+CGG 0.499358 5.2:-15963230 None:intergenic
TCCCTACGACACCGACAACT+TGG 0.500531 5.2:-15963522 None:intergenic
TCTCTCCATCGTATGCTCAA+TGG 0.500819 5.2:-15963042 None:intergenic
TTAATGATTTCACATTGTGT+TGG 0.501774 5.2:-15962141 None:intergenic
TTTGATGGATCGGAACCTAC+TGG 0.501938 5.2:-15962864 None:intergenic
GTCGCCGGAGTTTGAGATTG+TGG 0.504977 5.2:+15963430 MS.gene82473:CDS
TACAAAATCGAAAACGAAGC+TGG 0.505480 5.2:+15962333 MS.gene82473:CDS
AGTAATGCTGGGAGTGATGA+TGG 0.506662 5.2:-15962003 None:intergenic
GGTTTGTTTATGCGGCAATT+GGG 0.511453 5.2:+15963198 MS.gene82473:CDS
ACTGCTCATCCTAAAATTGA+CGG 0.512038 5.2:+15962624 MS.gene82473:CDS
ATGATTTCACATTGTGTTGG+AGG 0.513822 5.2:-15962138 None:intergenic
GGAGTTTGAGATTGTGGCGG+AGG 0.515266 5.2:+15963436 MS.gene82473:CDS
TGGATGAGATGTTAAAGGGA+GGG 0.519687 5.2:-15961849 None:intergenic
CACCAAGTTGTCGGTGTCGT+AGG 0.521647 5.2:+15963520 MS.gene82473:CDS
CACGCCATTACAATCTCAAA+CGG 0.522637 5.2:+15962270 MS.gene82473:CDS
TCAGCACGAAACCTGGACTT+CGG 0.523883 5.2:+15963140 MS.gene82473:CDS
GCGAAATCATGAAGAAAAGA+AGG 0.525570 5.2:-15962765 None:intergenic
ACCAAGTTGTCGGTGTCGTA+GGG 0.528287 5.2:+15963521 MS.gene82473:CDS
CCATCAAAAGTTTCAAGGAT+CGG 0.531863 5.2:+15962879 MS.gene82473:CDS
GAGGGAGGATGGTGAAGAAG+AGG 0.538581 5.2:+15963334 MS.gene82473:CDS
GTGTTTACGGATATTCAGCT+TGG 0.543147 5.2:+15962807 MS.gene82473:CDS
TGATTTCACATTGTGTTGGA+GGG 0.543631 5.2:-15962137 None:intergenic
GTGTTGGAGGGAGTTCGTCT+AGG 0.545453 5.2:-15962125 None:intergenic
ATGAGAAGAAGGGAGAGTCT+AGG 0.545870 5.2:+15963387 MS.gene82473:CDS
TCATGCACGCGATCAATGCG+TGG 0.547541 5.2:+15962967 MS.gene82473:CDS
GAAATCAAGCTTACACCAAA+CGG 0.548084 5.2:+15962546 MS.gene82473:CDS
ATTTGTAAGTTTGAACGTAA+CGG 0.551232 5.2:-15962311 None:intergenic
TATGACACGTGCTGCGGTGA+CGG 0.553634 5.2:-15962421 None:intergenic
GGAGGGAGTTCGTCTAGGAC+TGG 0.561664 5.2:-15962120 None:intergenic
GGTGATGGATGCAAAGTCGC+CGG 0.561761 5.2:+15963415 MS.gene82473:CDS
TAAGCTTGATTTCATAAGGA+AGG 0.561782 5.2:-15962536 None:intergenic
ATGGATGAGATGTTAAAGGG+AGG 0.562849 5.2:-15961850 None:intergenic
TACCACCTCTTCGACGGTGA+TGG 0.565615 5.2:+15962240 MS.gene82473:CDS
GGTGGTAAGGTCCACGTGGA+AGG 0.567364 5.2:-15962224 None:intergenic
ACATGTCTTGGATCTACAGA+TGG 0.569645 5.2:-15962078 None:intergenic
GGAGGGATAGAAGTTGTTGG+TGG 0.570969 5.2:-15961832 None:intergenic
TGCCATCACCGTCGAAGAGG+TGG 0.571502 5.2:-15962242 None:intergenic
GAGTGACATCACCAAGTTGT+CGG 0.571610 5.2:+15963511 MS.gene82473:CDS
TCACCGTCGAAGAGGTGGTA+AGG 0.575068 5.2:-15962237 None:intergenic
TCCGCCACAATCTCAAACTC+CGG 0.575111 5.2:-15963434 None:intergenic
CTTATAGCTTCTGCTGCACG+TGG 0.575305 5.2:+15962396 MS.gene82473:CDS
AGGATGAGCAGTCATACTCA+TGG 0.575328 5.2:-15962613 None:intergenic
AACGTAACGGCTACAGAGTG+TGG 0.575577 5.2:-15962298 None:intergenic
GCGTGCATGATATTAAATCC+TGG 0.576415 5.2:-15962954 None:intergenic
GGTCTTGTCATGGAGAATAC+CGG 0.577212 5.2:-15962744 None:intergenic
TATGTGCATGATGAGAAGAA+GGG 0.578787 5.2:+15963377 MS.gene82473:CDS
AAGAAAAGAAGGTCTTGTCA+TGG 0.578816 5.2:-15962754 None:intergenic
AAACAAAGCCAAACTTGTGT+TGG 0.580683 5.2:-15962481 None:intergenic
ATGTAAGCGCCGTTAAGTGA+CGG 0.584811 5.2:-15962177 None:intergenic
AACAATCCATGAAAACCATA+CGG 0.587297 5.2:-15963479 None:intergenic
AATGTGTTGAGTAGTAATGC+TGG 0.590033 5.2:-15962015 None:intergenic
CCACCAGAAATCATGTCAAG+AGG 0.590568 5.2:-15962837 None:intergenic
TGATAAATGGTGATTATATG+GGG 0.591790 5.2:+15963165 MS.gene82473:CDS
GATGGTGAAGAAGAGGATGA+TGG 0.592671 5.2:+15963341 MS.gene82473:CDS
CGGAGGTGAAGTTGCCAAGG+AGG 0.593842 5.2:+15963453 MS.gene82473:CDS
GTGGGATGGGGAAGATGATG+AGG 0.595645 5.2:+15962986 MS.gene82473:CDS
GGAGGTGAAGTTGCCAAGGA+GGG 0.600925 5.2:+15963454 MS.gene82473:CDS
TTGCCAAGGAGGGTGCCGTA+TGG 0.604907 5.2:+15963464 MS.gene82473:CDS
GTTTGGGATTGTGATGTTTG+GGG 0.605700 5.2:-15961889 None:intergenic
GTGTTGAAAGGTGAGGAAGT+TGG 0.610000 5.2:+15963260 MS.gene82473:CDS
GGACAATACAATCCTTCTAA+CGG 0.610598 5.2:+15962447 MS.gene82473:CDS
TGGCGGAGGTGAAGTTGCCA+AGG 0.613374 5.2:+15963450 MS.gene82473:CDS
TATGGAGAGGATTGTTATGG+TGG 0.621894 5.2:+15963293 MS.gene82473:CDS
GTCCACGTGGAAGGAACTGA+GGG 0.624509 5.2:-15962215 None:intergenic
GCATGCCATCACCGTCGAAG+AGG 0.632272 5.2:-15962245 None:intergenic
GTGAAGATTGATGTGTTGAA+AGG 0.637036 5.2:+15963248 MS.gene82473:CDS
CATGCACGCGATCAATGCGT+GGG 0.637360 5.2:+15962968 MS.gene82473:CDS
GGTCCACGTGGAAGGAACTG+AGG 0.638187 5.2:-15962216 None:intergenic
GGACCTTACCACCTCTTCGA+CGG 0.645380 5.2:+15962234 MS.gene82473:CDS
TGGATGTAGATTGTATGGAG+AGG 0.648715 5.2:+15963280 MS.gene82473:CDS
GCCGGAGTTTGAGATTGTGG+CGG 0.652926 5.2:+15963433 MS.gene82473:CDS
TGAAGATAAATATTAAAACA+GGG 0.655068 5.2:+15963096 MS.gene82473:CDS
AACCCTCAGTTCCTTCCACG+TGG 0.655473 5.2:+15962213 MS.gene82473:CDS
GATTGATGTGTTGAAAGGTG+AGG 0.662195 5.2:+15963253 MS.gene82473:CDS
AATGCCGAAGATTTCAGGAG+TGG 0.663954 5.2:+15963226 MS.gene82473:CDS
TCCAGATATGACACGTGCTG+CGG 0.676934 5.2:-15962427 None:intergenic
ATGTGTTGAGTAGTAATGCT+GGG 0.681095 5.2:-15962014 None:intergenic
AGCACGAAACCTGGACTTCG+GGG 0.694520 5.2:+15963142 MS.gene82473:CDS
TCTCTCCATTGAGCATACGA+TGG 0.695768 5.2:+15963037 MS.gene82473:CDS
TAAGCGCCGTTAAGTGACGG+TGG 0.696353 5.2:-15962174 None:intergenic
ATGGTGGCATAGGACCGTAA+CGG 0.707951 5.2:-15962671 None:intergenic
GACTCGTCACTAGCATAACG+AGG 0.742544 5.2:-15962909 None:intergenic
AAGAGGTGGTAAGGTCCACG+TGG 0.744047 5.2:-15962228 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGAAGATAAATATTAAAACA+GGG + chr5.2:15963096-15963115 MS.gene82473:CDS 15.0%
!! GTGAAGATAAATATTAAAAC+AGG + chr5.2:15963095-15963114 MS.gene82473:CDS 20.0%
! AAACGGAAATAGGTTAAAAA+TGG - chr5.2:15962693-15962712 None:intergenic 25.0%
! ACACAATGTGAAATCATTAA+AGG + chr5.2:15962144-15962163 MS.gene82473:CDS 25.0%
! ATTTGTAAGTTTGAACGTAA+CGG - chr5.2:15962314-15962333 None:intergenic 25.0%
! GTGATAAATGGTGATTATAT+GGG + chr5.2:15963164-15963183 MS.gene82473:CDS 25.0%
! TGATAAATGGTGATTATATG+GGG + chr5.2:15963165-15963184 MS.gene82473:CDS 25.0%
! TTAATGATTTCACATTGTGT+TGG - chr5.2:15962144-15962163 None:intergenic 25.0%
!! TAAAAAGTATGCTGTGTTTA+CGG + chr5.2:15962794-15962813 MS.gene82473:CDS 25.0%
!!! TGCTTACTTGTTATTATGAT+GGG - chr5.2:15961811-15961830 None:intergenic 25.0%
!!! TTGCTTACTTGTTATTATGA+TGG - chr5.2:15961812-15961831 None:intergenic 25.0%
!!! TTGTTATTATGATGGGTTTT+GGG - chr5.2:15961804-15961823 None:intergenic 25.0%
AACAATCCATGAAAACCATA+CGG - chr5.2:15963482-15963501 None:intergenic 30.0%
ATTATATGGGGAAGAAGAAT+AGG + chr5.2:15963177-15963196 MS.gene82473:CDS 30.0%
ATTTGCATCAAAACGGAAAT+AGG - chr5.2:15962703-15962722 None:intergenic 30.0%
GGTGATAAATGGTGATTATA+TGG + chr5.2:15963163-15963182 MS.gene82473:CDS 30.0%
TAACACCATTTGCATCAAAA+CGG - chr5.2:15962710-15962729 None:intergenic 30.0%
TAAGCTTGATTTCATAAGGA+AGG - chr5.2:15962539-15962558 None:intergenic 30.0%
TGTTAAACATAACGATGTTC+CGG + chr5.2:15962725-15962744 MS.gene82473:CDS 30.0%
! GAAGAATAGGTTTGTTTATG+CGG + chr5.2:15963190-15963209 MS.gene82473:CDS 30.0%
! TTGATGGATGAGATGTTAAA+GGG - chr5.2:15961856-15961875 None:intergenic 30.0%
! TTTGATGGATGAGATGTTAA+AGG - chr5.2:15961857-15961876 None:intergenic 30.0%
!! CACAGCATACTTTTTAGTAA+TGG - chr5.2:15962790-15962809 None:intergenic 30.0%
!! TATTTCCGTTTTGATGCAAA+TGG + chr5.2:15962702-15962721 MS.gene82473:CDS 30.0%
!! TTTTCATGGATTGTTTGTGA+AGG + chr5.2:15963487-15963506 MS.gene82473:CDS 30.0%
!! TTTTCCGTTTGAGATTGTAA+TGG - chr5.2:15962277-15962296 None:intergenic 30.0%
!!! CTTGTTATTATGATGGGTTT+TGG - chr5.2:15961805-15961824 None:intergenic 30.0%
!!! TTGTGGTTTTTTGAGGTTAT+GGG - chr5.2:15961980-15961999 None:intergenic 30.0%
!!! TTTGTGGTTTTTTGAGGTTA+TGG - chr5.2:15961981-15962000 None:intergenic 30.0%
!!! TTTTGAGACAAAACATGTCT+TGG - chr5.2:15962093-15962112 None:intergenic 30.0%
AAACAAAGCCAAACTTGTGT+TGG - chr5.2:15962484-15962503 None:intergenic 35.0%
AACGGTGATATCCAAACAAT+CGG + chr5.2:15962564-15962583 MS.gene82473:CDS 35.0%
AATCCTCTTGACATGATTTC+TGG + chr5.2:15962834-15962853 MS.gene82473:CDS 35.0%
AATGTGTTGAGTAGTAATGC+TGG - chr5.2:15962018-15962037 None:intergenic 35.0%
ACTGCTCATCCTAAAATTGA+CGG + chr5.2:15962624-15962643 MS.gene82473:CDS 35.0%
ATGATTTCACATTGTGTTGG+AGG - chr5.2:15962141-15962160 None:intergenic 35.0%
ATGTGTTGAGTAGTAATGCT+GGG - chr5.2:15962017-15962036 None:intergenic 35.0%
ATTGTTTGGATATCACCGTT+TGG - chr5.2:15962564-15962583 None:intergenic 35.0%
CCATCAAAAGTTTCAAGGAT+CGG + chr5.2:15962879-15962898 MS.gene82473:CDS 35.0%
CGGAAATAGGTTAAAAATGG+TGG - chr5.2:15962690-15962709 None:intergenic 35.0%
GAAATCAAGCTTACACCAAA+CGG + chr5.2:15962546-15962565 MS.gene82473:CDS 35.0%
GAAGTTGGATGTAGATTGTA+TGG + chr5.2:15963275-15963294 MS.gene82473:CDS 35.0%
GCGAAATCATGAAGAAAAGA+AGG - chr5.2:15962768-15962787 None:intergenic 35.0%
GGACAATACAATCCTTCTAA+CGG + chr5.2:15962447-15962466 MS.gene82473:CDS 35.0%
GGTGTAAGCTTGATTTCATA+AGG - chr5.2:15962543-15962562 None:intergenic 35.0%
GTGAAGATTGATGTGTTGAA+AGG + chr5.2:15963248-15963267 MS.gene82473:CDS 35.0%
GTTTGCCGTTAAAATCTTGA+CGG - chr5.2:15962590-15962609 None:intergenic 35.0%
TACAAAATCGAAAACGAAGC+TGG + chr5.2:15962333-15962352 MS.gene82473:CDS 35.0%
TAGGTTAAAAATGGTGGCAT+AGG - chr5.2:15962684-15962703 None:intergenic 35.0%
TATGTGCATGATGAGAAGAA+GGG + chr5.2:15963377-15963396 MS.gene82473:CDS 35.0%
TGAAACCAGAAAACACGTTT+GGG - chr5.2:15962371-15962390 None:intergenic 35.0%
TGATTTCACATTGTGTTGGA+GGG - chr5.2:15962140-15962159 None:intergenic 35.0%
TTATGTGCATGATGAGAAGA+AGG + chr5.2:15963376-15963395 MS.gene82473:CDS 35.0%
TTGAAACCAGAAAACACGTT+TGG - chr5.2:15962372-15962391 None:intergenic 35.0%
TTGTATGGAGAGGATTGTTA+TGG + chr5.2:15963290-15963309 MS.gene82473:CDS 35.0%
! AAAACACGTTTGGGATAAGT+TGG - chr5.2:15962362-15962381 None:intergenic 35.0%
! AAGAAAAGAAGGTCTTGTCA+TGG - chr5.2:15962757-15962776 None:intergenic 35.0%
! GAAACTTTTGCTTTCCGTTA+CGG + chr5.2:15962657-15962676 MS.gene82473:CDS 35.0%
! GGTAACAAACTTTTTGCGTT+AGG + chr5.2:15962504-15962523 MS.gene82473:CDS 35.0%
! TGTTTGGGATTGTGATGTTT+GGG - chr5.2:15961893-15961912 None:intergenic 35.0%
!! AACACAAGTTTGGCTTTGTT+TGG + chr5.2:15962483-15962502 MS.gene82473:CDS 35.0%
!! CCTTGAAACTTTTGATGGAT+CGG - chr5.2:15962877-15962896 None:intergenic 35.0%
!! TGATGTTTGGGGTTTTTCTT+TGG - chr5.2:15961881-15961900 None:intergenic 35.0%
!! TTTTATTGGTGGGTCTATGA+AGG - chr5.2:15962058-15962077 None:intergenic 35.0%
!!! ATGGTGTTTGTGGTTTTTTG+AGG - chr5.2:15961987-15962006 None:intergenic 35.0%
!!! CTACAGATGGTTTTATTGGT+GGG - chr5.2:15962068-15962087 None:intergenic 35.0%
!!! GGATCTACAGATGGTTTTAT+TGG - chr5.2:15962072-15962091 None:intergenic 35.0%
!!! GGGTTTTTCTTTGGTTTTGA+TGG - chr5.2:15961872-15961891 None:intergenic 35.0%
!!! TCTACAGATGGTTTTATTGG+TGG - chr5.2:15962069-15962088 None:intergenic 35.0%
AAACTTCCACCGTCACTTAA+CGG + chr5.2:15962168-15962187 MS.gene82473:CDS 40.0%
AACGGCGCTTACATAAGAAA+TGG + chr5.2:15962186-15962205 MS.gene82473:CDS 40.0%
AATCCAATGCCGAAGATTTC+AGG + chr5.2:15963221-15963240 MS.gene82473:CDS 40.0%
ACATGTCTTGGATCTACAGA+TGG - chr5.2:15962081-15962100 None:intergenic 40.0%
ATGGATGAGATGTTAAAGGG+AGG - chr5.2:15961853-15961872 None:intergenic 40.0%
CACGCCATTACAATCTCAAA+CGG + chr5.2:15962270-15962289 MS.gene82473:CDS 40.0%
CCGATCCATCAAAAGTTTCA+AGG + chr5.2:15962874-15962893 MS.gene82473:CDS 40.0%
GATTGATGTGTTGAAAGGTG+AGG + chr5.2:15963253-15963272 MS.gene82473:CDS 40.0%
GCGTGCATGATATTAAATCC+TGG - chr5.2:15962957-15962976 None:intergenic 40.0%
GTGTTTACGGATATTCAGCT+TGG + chr5.2:15962807-15962826 MS.gene82473:CDS 40.0%
TACAATCCTTCTAACGGCAT+TGG + chr5.2:15962453-15962472 MS.gene82473:CDS 40.0%
TATGGAGAGGATTGTTATGG+TGG + chr5.2:15963293-15963312 MS.gene82473:CDS 40.0%
TGCTCAATGGAGAGAACATT+CGG - chr5.2:15963032-15963051 None:intergenic 40.0%
TGGATGAGATGTTAAAGGGA+GGG - chr5.2:15961852-15961871 None:intergenic 40.0%
TGGATGTAGATTGTATGGAG+AGG + chr5.2:15963280-15963299 MS.gene82473:CDS 40.0%
TGGTTTAGCCAACACAAGTT+TGG + chr5.2:15962473-15962492 MS.gene82473:CDS 40.0%
TTCACCACTCCTGAAATCTT+CGG - chr5.2:15963233-15963252 None:intergenic 40.0%
! AGGTTTGTTTATGCGGCAAT+TGG + chr5.2:15963197-15963216 MS.gene82473:CDS 40.0%
! ATCGGCCGTCAAGATTTTAA+CGG + chr5.2:15962582-15962601 MS.gene82473:CDS 40.0%
! CCGATCCTTGAAACTTTTGA+TGG - chr5.2:15962882-15962901 None:intergenic 40.0%
! CTTATCCCAAACGTGTTTTC+TGG + chr5.2:15962363-15962382 MS.gene82473:CDS 40.0%
! GGTGTAGGAAGAGTTTTTGT+GGG - chr5.2:15961940-15961959 None:intergenic 40.0%
! GGTTTGTTTATGCGGCAATT+GGG + chr5.2:15963198-15963217 MS.gene82473:CDS 40.0%
! GTGTAGGAAGAGTTTTTGTG+GGG - chr5.2:15961939-15961958 None:intergenic 40.0%
! GTGTTTGGGATTGTGATGTT+TGG - chr5.2:15961894-15961913 None:intergenic 40.0%
! GTTTGGGATTGTGATGTTTG+GGG - chr5.2:15961892-15961911 None:intergenic 40.0%
! TGTAGGAAGAGTTTTTGTGG+GGG - chr5.2:15961938-15961957 None:intergenic 40.0%
!!! AGAGTCTAGGTTTTTGGTGA+TGG + chr5.2:15963400-15963419 MS.gene82473:CDS 40.0%
!!! GGTGGTGGTTTTTAGTGTTT+GGG - chr5.2:15961908-15961927 None:intergenic 40.0%
!!! TGGTGGTGGTTTTTAGTGTT+TGG - chr5.2:15961909-15961928 None:intergenic 40.0%
!!! TGGTTTTTTGAGGTTATGGG+TGG - chr5.2:15961977-15961996 None:intergenic 40.0%
!!! TTTTTTGAGGTTATGGGTGG+TGG - chr5.2:15961974-15961993 None:intergenic 40.0%
AAGGGAGGGATAGAAGTTGT+TGG - chr5.2:15961838-15961857 None:intergenic 45.0%
AATCTTGACGGCCGATTGTT+TGG - chr5.2:15962578-15962597 None:intergenic 45.0%
AATGCCGAAGATTTCAGGAG+TGG + chr5.2:15963226-15963245 MS.gene82473:CDS 45.0%
ACCATTATCAGCACGAAACC+TGG + chr5.2:15963133-15963152 MS.gene82473:CDS 45.0%
ACTCCTGAAATCTTCGGCAT+TGG - chr5.2:15963227-15963246 None:intergenic 45.0%
AGGATGAGCAGTCATACTCA+TGG - chr5.2:15962616-15962635 None:intergenic 45.0%
AGTAATGCTGGGAGTGATGA+TGG - chr5.2:15962006-15962025 None:intergenic 45.0%
ATGAAATGGTTCGATGTGCC+AGG + chr5.2:15962936-15962955 MS.gene82473:CDS 45.0%
ATGAGAAGAAGGGAGAGTCT+AGG + chr5.2:15963387-15963406 MS.gene82473:CDS 45.0%
CCACCAGAAATCATGTCAAG+AGG - chr5.2:15962840-15962859 None:intergenic 45.0%
GAGTGACATCACCAAGTTGT+CGG + chr5.2:15963511-15963530 MS.gene82473:CDS 45.0%
GATGGTGAAGAAGAGGATGA+TGG + chr5.2:15963341-15963360 MS.gene82473:CDS 45.0%
GCTAAACCAATGCCGTTAGA+AGG - chr5.2:15962462-15962481 None:intergenic 45.0%
GTGACGAGTCGAAGATGAAA+TGG + chr5.2:15962922-15962941 MS.gene82473:CDS 45.0%
GTGTTGAAAGGTGAGGAAGT+TGG + chr5.2:15963260-15963279 MS.gene82473:CDS 45.0%
TCTCTCCATCGTATGCTCAA+TGG - chr5.2:15963045-15963064 None:intergenic 45.0%
TCTCTCCATTGAGCATACGA+TGG + chr5.2:15963037-15963056 MS.gene82473:CDS 45.0%
! ATGTAAGCGCCGTTAAGTGA+CGG - chr5.2:15962180-15962199 None:intergenic 45.0%
! CCTCTTGACATGATTTCTGG+TGG + chr5.2:15962837-15962856 MS.gene82473:CDS 45.0%
! GAAGGGAGAGTCTAGGTTTT+TGG + chr5.2:15963394-15963413 MS.gene82473:CDS 45.0%
! GGGTGTAGGAAGAGTTTTTG+TGG - chr5.2:15961941-15961960 None:intergenic 45.0%
! GGTCTTGTCATGGAGAATAC+CGG - chr5.2:15962747-15962766 None:intergenic 45.0%
! GTAGGAAGAGTTTTTGTGGG+GGG - chr5.2:15961937-15961956 None:intergenic 45.0%
! TCCAGGTTTCGTGCTGATAA+TGG - chr5.2:15963137-15963156 None:intergenic 45.0%
! TTTGATGGATCGGAACCTAC+TGG - chr5.2:15962867-15962886 None:intergenic 45.0%
!! ATTTCTGGTGGTTCTCCAGT+AGG + chr5.2:15962849-15962868 MS.gene82473:CDS 45.0%
AACGTAACGGCTACAGAGTG+TGG - chr5.2:15962301-15962320 None:intergenic 50.0%
ATGGTGGCATAGGACCGTAA+CGG - chr5.2:15962674-15962693 None:intergenic 50.0%
CATTTATCACCCCGAAGTCC+AGG - chr5.2:15963154-15963173 None:intergenic 50.0%
CCTAAAATTGACGGCGACAC+TGG + chr5.2:15962633-15962652 MS.gene82473:CDS 50.0%
CTGGACTTCGGGGTGATAAA+TGG + chr5.2:15963152-15963171 MS.gene82473:CDS 50.0%
CTTATAGCTTCTGCTGCACG+TGG + chr5.2:15962396-15962415 MS.gene82473:CDS 50.0%
GACTCGTCACTAGCATAACG+AGG - chr5.2:15962912-15962931 None:intergenic 50.0%
GGAGGGATAGAAGTTGTTGG+TGG - chr5.2:15961835-15961854 None:intergenic 50.0%
GTGGAAGGAACTGAGGGTTT+GGG - chr5.2:15962212-15962231 None:intergenic 50.0%
TCAGCACGAAACCTGGACTT+CGG + chr5.2:15963140-15963159 MS.gene82473:CDS 50.0%
TCCAGATATGACACGTGCTG+CGG - chr5.2:15962430-15962449 None:intergenic 50.0%
TCCGCCACAATCTCAAACTC+CGG - chr5.2:15963437-15963456 None:intergenic 50.0%
TCCTCCCTCACCACGAAAAA+CGG - chr5.2:15963323-15963342 None:intergenic 50.0%
! CCAGTGTCGCCGTCAATTTT+AGG - chr5.2:15962636-15962655 None:intergenic 50.0%
! TTTGAGGTTATGGGTGGTGG+TGG - chr5.2:15961971-15961990 None:intergenic 50.0%
! TTTTTCGTGGTGAGGGAGGA+TGG + chr5.2:15963323-15963342 MS.gene82473:CDS 50.0%
!! AGTTTTTGTGGGGGGTGTAG+TGG - chr5.2:15961929-15961948 None:intergenic 50.0%
!! GGGAGTGATGATGGTGTTTG+TGG - chr5.2:15961997-15962016 None:intergenic 50.0%
!!! AGGGTGCCGTATGGTTTTCA+TGG + chr5.2:15963473-15963492 MS.gene82473:CDS 50.0%
AAACCATACGGCACCCTCCT+TGG - chr5.2:15963470-15963489 None:intergenic 55.0%
AACCCTCAGTTCCTTCCACG+TGG + chr5.2:15962213-15962232 MS.gene82473:CDS 55.0%
AAGAGGTGGTAAGGTCCACG+TGG - chr5.2:15962231-15962250 None:intergenic 55.0%
ACCGCAGCACGTGTCATATC+TGG + chr5.2:15962426-15962445 MS.gene82473:CDS 55.0%
ACGCGATCAATGCGTGGGAT+GGG + chr5.2:15962973-15962992 MS.gene82473:CDS 55.0%
AGCACGAAACCTGGACTTCG+GGG + chr5.2:15963142-15963161 MS.gene82473:CDS 55.0%
CAGCACGAAACCTGGACTTC+GGG + chr5.2:15963141-15963160 MS.gene82473:CDS 55.0%
CATGCACGCGATCAATGCGT+GGG + chr5.2:15962968-15962987 MS.gene82473:CDS 55.0%
CGTGGAAGGAACTGAGGGTT+TGG - chr5.2:15962213-15962232 None:intergenic 55.0%
GAGGGAGGATGGTGAAGAAG+AGG + chr5.2:15963334-15963353 MS.gene82473:CDS 55.0%
GGACCTTACCACCTCTTCGA+CGG + chr5.2:15962234-15962253 MS.gene82473:CDS 55.0%
GGAGGTGAAGTTGCCAAGGA+GGG + chr5.2:15963454-15963473 MS.gene82473:CDS 55.0%
GTCCACGTGGAAGGAACTGA+GGG - chr5.2:15962218-15962237 None:intergenic 55.0%
GTGGGATGGGGAAGATGATG+AGG + chr5.2:15962986-15963005 MS.gene82473:CDS 55.0%
GTGTTGGAGGGAGTTCGTCT+AGG - chr5.2:15962128-15962147 None:intergenic 55.0%
TACCACCTCTTCGACGGTGA+TGG + chr5.2:15962240-15962259 MS.gene82473:CDS 55.0%
TATGACACGTGCTGCGGTGA+CGG - chr5.2:15962424-15962443 None:intergenic 55.0%
TCACCGTCGAAGAGGTGGTA+AGG - chr5.2:15962240-15962259 None:intergenic 55.0%
TCATGCACGCGATCAATGCG+TGG + chr5.2:15962967-15962986 MS.gene82473:CDS 55.0%
! GCCGGAGTTTGAGATTGTGG+CGG + chr5.2:15963433-15963452 MS.gene82473:CDS 55.0%
! GGAGTTTGAGATTGTGGCGG+AGG + chr5.2:15963436-15963455 MS.gene82473:CDS 55.0%
! GTCGCCGGAGTTTGAGATTG+TGG + chr5.2:15963430-15963449 MS.gene82473:CDS 55.0%
! TAAGCGCCGTTAAGTGACGG+TGG - chr5.2:15962177-15962196 None:intergenic 55.0%
!! GGTGATGGATGCAAAGTCGC+CGG + chr5.2:15963415-15963434 MS.gene82473:CDS 55.0%
!! TTATGGGTGGTGGTGGTGGT+GGG - chr5.2:15961964-15961983 None:intergenic 55.0%
!! TTTTGTGGGGGGTGTAGTGG+TGG - chr5.2:15961926-15961945 None:intergenic 55.0%
!!! CGAGCCGTTTTTCGTGGTGA+GGG + chr5.2:15963316-15963335 MS.gene82473:CDS 55.0%
CACGCGATCAATGCGTGGGA+TGG + chr5.2:15962972-15962991 MS.gene82473:CDS 60.0%
CGCGATCAATGCGTGGGATG+GGG + chr5.2:15962974-15962993 MS.gene82473:CDS 60.0%
CGGAGGTGAAGTTGCCAAGG+AGG + chr5.2:15963453-15963472 MS.gene82473:CDS 60.0%
GCATGCCATCACCGTCGAAG+AGG - chr5.2:15962248-15962267 None:intergenic 60.0%
GGTCCACGTGGAAGGAACTG+AGG - chr5.2:15962219-15962238 None:intergenic 60.0%
GGTGGTAAGGTCCACGTGGA+AGG - chr5.2:15962227-15962246 None:intergenic 60.0%
TGCCATCACCGTCGAAGAGG+TGG - chr5.2:15962245-15962264 None:intergenic 60.0%
TGGCGGAGGTGAAGTTGCCA+AGG + chr5.2:15963450-15963469 MS.gene82473:CDS 60.0%
! GGAGGGAGTTCGTCTAGGAC+TGG - chr5.2:15962123-15962142 None:intergenic 60.0%
!! GAGGTTATGGGTGGTGGTGG+TGG - chr5.2:15961968-15961987 None:intergenic 60.0%
!! GTTATGGGTGGTGGTGGTGG+TGG - chr5.2:15961965-15961984 None:intergenic 60.0%
!! TATGGGTGGTGGTGGTGGTG+GGG - chr5.2:15961963-15961982 None:intergenic 60.0%
!! TTGCCAAGGAGGGTGCCGTA+TGG + chr5.2:15963464-15963483 MS.gene82473:CDS 60.0%
!!! GCCGTTTTTCGTGGTGAGGG+AGG + chr5.2:15963319-15963338 MS.gene82473:CDS 60.0%
!!! GCGAGCCGTTTTTCGTGGTG+AGG + chr5.2:15963315-15963334 MS.gene82473:CDS 60.0%
!!! TGGTGGCGAGCCGTTTTTCG+TGG + chr5.2:15963310-15963329 MS.gene82473:CDS 60.0%
! TGTGGGGGGTGTAGTGGTGG+TGG - chr5.2:15961923-15961942 None:intergenic 65.0%
!! ATGGGTGGTGGTGGTGGTGG+GGG - chr5.2:15961962-15961981 None:intergenic 65.0%
!! GTGGTGGTGGTGGGGGGTGT+AGG - chr5.2:15961955-15961974 None:intergenic 70.0%
!! TGGGTGGTGGTGGTGGTGGG+GGG - chr5.2:15961961-15961980 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 15961796 15963541 15961796 ID=MS.gene82473
chr5.2 mRNA 15961796 15963541 15961796 ID=MS.gene82473.t1;Parent=MS.gene82473
chr5.2 exon 15961796 15963541 15961796 ID=MS.gene82473.t1.exon1;Parent=MS.gene82473.t1
chr5.2 CDS 15961796 15963541 15961796 ID=cds.MS.gene82473.t1;Parent=MS.gene82473.t1
Gene Sequence

>MS.gene82473

ATGGTCCCAAAACCCATCATAATAACAAGTAAGCAACCACCAACAACTTCTATCCCTCCCTTTAACATCTCATCCATCAAAACCAAAGAAAAACCCCAAACATCACAATCCCAAACACTAAAAACCACCACCACTACACCCCCCACAAAAACTCTTCCTACACCCCCCACCACCACCACCACCCATAACCTCAAAAAACCACAAACACCATCATCACTCCCAGCATTACTACTCAACACATTCGACGACATCATCAACACCTTCATAGACCCACCAATAAAACCATCTGTAGATCCAAGACATGTTTTGTCTCAAAATTTTGCACCAGTCCTAGACGAACTCCCTCCAACACAATGTGAAATCATTAAAGGCAAACTTCCACCGTCACTTAACGGCGCTTACATAAGAAATGGCCCAAACCCTCAGTTCCTTCCACGTGGACCTTACCACCTCTTCGACGGTGATGGCATGCTTCACGCCATTACAATCTCAAACGGAAAAGCCACACTCTGTAGCCGTTACGTTCAAACTTACAAATACAAAATCGAAAACGAAGCTGGTTACCAACTTATCCCAAACGTGTTTTCTGGTTTCAACTCGCTTATAGCTTCTGCTGCACGTGGCTCCGTCACCGCAGCACGTGTCATATCTGGACAATACAATCCTTCTAACGGCATTGGTTTAGCCAACACAAGTTTGGCTTTGTTTGGTAACAAACTTTTTGCGTTAGGAGAATCAGACCTTCCTTATGAAATCAAGCTTACACCAAACGGTGATATCCAAACAATCGGCCGTCAAGATTTTAACGGCAAACTCTCCATGAGTATGACTGCTCATCCTAAAATTGACGGCGACACTGGTGAAACTTTTGCTTTCCGTTACGGTCCTATGCCACCATTTTTAACCTATTTCCGTTTTGATGCAAATGGTGTTAAACATAACGATGTTCCGGTATTCTCCATGACAAGACCTTCTTTTCTTCATGATTTCGCCATTACTAAAAAGTATGCTGTGTTTACGGATATTCAGCTTGGAATGAATCCTCTTGACATGATTTCTGGTGGTTCTCCAGTAGGTTCCGATCCATCAAAAGTTTCAAGGATCGGAATTATACCTCGTTATGCTAGTGACGAGTCGAAGATGAAATGGTTCGATGTGCCAGGATTTAATATCATGCACGCGATCAATGCGTGGGATGGGGAAGATGATGAGGATACTGTGACACTAATTGCTCCGAATGTTCTCTCCATTGAGCATACGATGGAGAGACTTGAGCTTGTGCATGCAATGATTGAGAAAGTGAAGATAAATATTAAAACAGGGATAGTATCAAGACAACCATTATCAGCACGAAACCTGGACTTCGGGGTGATAAATGGTGATTATATGGGGAAGAAGAATAGGTTTGTTTATGCGGCAATTGGGAATCCAATGCCGAAGATTTCAGGAGTGGTGAAGATTGATGTGTTGAAAGGTGAGGAAGTTGGATGTAGATTGTATGGAGAGGATTGTTATGGTGGCGAGCCGTTTTTCGTGGTGAGGGAGGATGGTGAAGAAGAGGATGATGGTTATTTAGTGAGTTATGTGCATGATGAGAAGAAGGGAGAGTCTAGGTTTTTGGTGATGGATGCAAAGTCGCCGGAGTTTGAGATTGTGGCGGAGGTGAAGTTGCCAAGGAGGGTGCCGTATGGTTTTCATGGATTGTTTGTGAAGGAGAGTGACATCACCAAGTTGTCGGTGTCGTAG
Protein sequence

>MS.gene82473.t1

MVPKPIIITSKQPPTTSIPPFNISSIKTKEKPQTSQSQTLKTTTTTPPTKTLPTPPTTTTTHNLKKPQTPSSLPALLLNTFDDIINTFIDPPIKPSVDPRHVLSQNFAPVLDELPPTQCEIIKGKLPPSLNGAYIRNGPNPQFLPRGPYHLFDGDGMLHAITISNGKATLCSRYVQTYKYKIENEAGYQLIPNVFSGFNSLIASAARGSVTAARVISGQYNPSNGIGLANTSLALFGNKLFALGESDLPYEIKLTPNGDIQTIGRQDFNGKLSMSMTAHPKIDGDTGETFAFRYGPMPPFLTYFRFDANGVKHNDVPVFSMTRPSFLHDFAITKKYAVFTDIQLGMNPLDMISGGSPVGSDPSKVSRIGIIPRYASDESKMKWFDVPGFNIMHAINAWDGEDDEDTVTLIAPNVLSIEHTMERLELVHAMIEKVKINIKTGIVSRQPLSARNLDFGVINGDYMGKKNRFVYAAIGNPMPKISGVVKIDVLKGEEVGCRLYGEDCYGGEPFFVVREDGEEEDDGYLVSYVHDEKKGESRFLVMDAKSPEFEIVAEVKLPRRVPYGFHGLFVKESDITKLSVS