AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene27117


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 30 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGATCTTTGGACAGATTTA+AGG 0.152281 1.1:+56279 None:intergenic
TTCAAAAGCTTCAGGATCTT+TGG 0.220610 1.1:+56267 None:intergenic
TTCAGCTCTTCAAAAGCTTC+AGG 0.290764 1.1:+56259 None:intergenic
TGCTTCAATCCCACAATATC+TGG 0.308295 1.1:+56366 None:intergenic
CCTTTCCACAACAACTTGCT+TGG 0.375325 1.1:-56121 MS.gene27117:CDS
CTTTCCACAACAACTTGCTT+GGG 0.383331 1.1:-56120 MS.gene27117:CDS
GGATCTTTGGACAGATTTAA+GGG 0.386269 1.1:+56280 None:intergenic
TTGGCAATGATGGCTTGGTT+AGG 0.434393 1.1:-56211 MS.gene27117:CDS
AAATGGACGCTTGGCAATGA+TGG 0.434782 1.1:-56221 MS.gene27117:CDS
GGATCAAACAATGCTCCTCC+AGG 0.460577 1.1:+56301 None:intergenic
CCAGAATACGCCAGATATTG+TGG 0.467314 1.1:-56376 None:intergenic
ATATTGTGGGATTGAAGCAC+TGG 0.493998 1.1:-56362 MS.gene27117:CDS
TTCACACAAGCGGCACTGAC+GGG 0.495505 1.1:-56184 MS.gene27117:CDS
CTTCACACAAGCGGCACTGA+CGG 0.499189 1.1:-56185 MS.gene27117:CDS
CCAGGGTAGTTTATATCACC+AGG 0.514510 1.1:+56319 None:intergenic
CAAGCGGCACTGACGGGTAA+AGG 0.516467 1.1:-56178 MS.gene27117:CDS
GACGCTTGGCAATGATGGCT+TGG 0.532277 1.1:-56216 MS.gene27117:CDS
CAATTCAATGAAATTACACT+AGG 0.563647 1.1:-56092 None:intergenic
GTGACCCAAGCAAGTTGTTG+TGG 0.567405 1.1:+56116 None:intergenic
CCAAGCAAGTTGTTGTGGAA+AGG 0.568516 1.1:+56121 None:intergenic
CCTCTAGGAATATATCTGCC+TGG 0.569936 1.1:-56337 MS.gene27117:CDS
ATTGAAGCACTGGAGCCTCT+AGG 0.571898 1.1:-56352 MS.gene27117:CDS
CAGAATACGCCAGATATTGT+GGG 0.576638 1.1:-56375 MS.gene27117:CDS
CAAGTTGTTGTGGAAAGGAT+CGG 0.585579 1.1:+56126 None:intergenic
GTTAGGTTTCTTCACACAAG+CGG 0.612558 1.1:-56194 MS.gene27117:CDS
AGGTCCTGTACAAAACATTG+TGG 0.632483 1.1:-56158 MS.gene27117:CDS
CCTGGTGATATAAACTACCC+TGG 0.634831 1.1:-56319 MS.gene27117:CDS
GATCAAACAATGCTCCTCCA+GGG 0.637834 1.1:+56302 None:intergenic
CCAGGCAGATATATTCCTAG+AGG 0.666249 1.1:+56337 None:intergenic
GGTGATATAAACTACCCTGG+AGG 0.752250 1.1:-56316 MS.gene27117:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGTGAAAGAAATAAAAAA+TGG - chr1.1:56227-56246 MS.gene27117:CDS 15.0%
AGAAATAAAAAATGGACGCT+TGG - chr1.1:56235-56254 MS.gene27117:CDS 30.0%
AGGATCTTTGGACAGATTTA+AGG + chr1.1:56189-56208 None:intergenic 35.0%
GGATCTTTGGACAGATTTAA+GGG + chr1.1:56188-56207 None:intergenic 35.0%
TTCAAAAGCTTCAGGATCTT+TGG + chr1.1:56201-56220 None:intergenic 35.0%
! TGATCCACAATGTTTTGTAC+AGG + chr1.1:56314-56333 None:intergenic 35.0%
AGGTCCTGTACAAAACATTG+TGG - chr1.1:56307-56326 MS.gene27117:CDS 40.0%
ATATTGTGGGATTGAAGCAC+TGG - chr1.1:56103-56122 MS.gene27117:CDS 40.0%
CAAGTTGTTGTGGAAAGGAT+CGG + chr1.1:56342-56361 None:intergenic 40.0%
GTTAGGTTTCTTCACACAAG+CGG - chr1.1:56271-56290 MS.gene27117:CDS 40.0%
TGCTTCAATCCCACAATATC+TGG + chr1.1:56102-56121 None:intergenic 40.0%
TTCAGCTCTTCAAAAGCTTC+AGG + chr1.1:56209-56228 None:intergenic 40.0%
! CTTTCCACAACAACTTGCTT+GGG - chr1.1:56345-56364 MS.gene27117:CDS 40.0%
AAATGGACGCTTGGCAATGA+TGG - chr1.1:56244-56263 MS.gene27117:CDS 45.0%
CCAAGCAAGTTGTTGTGGAA+AGG + chr1.1:56347-56366 None:intergenic 45.0%
CCAGGCAGATATATTCCTAG+AGG + chr1.1:56131-56150 None:intergenic 45.0%
CCAGGGTAGTTTATATCACC+AGG + chr1.1:56149-56168 None:intergenic 45.0%
CCTGGTGATATAAACTACCC+TGG - chr1.1:56146-56165 MS.gene27117:CDS 45.0%
GATCAAACAATGCTCCTCCA+GGG + chr1.1:56166-56185 None:intergenic 45.0%
GGTGATATAAACTACCCTGG+AGG - chr1.1:56149-56168 MS.gene27117:CDS 45.0%
! CCTCTAGGAATATATCTGCC+TGG - chr1.1:56128-56147 MS.gene27117:CDS 45.0%
! CCTTTCCACAACAACTTGCT+TGG - chr1.1:56344-56363 MS.gene27117:CDS 45.0%
!! TTGGCAATGATGGCTTGGTT+AGG - chr1.1:56254-56273 MS.gene27117:CDS 45.0%
GGATCAAACAATGCTCCTCC+AGG + chr1.1:56167-56186 None:intergenic 50.0%
GTGACCCAAGCAAGTTGTTG+TGG + chr1.1:56352-56371 None:intergenic 50.0%
!! ATTGAAGCACTGGAGCCTCT+AGG - chr1.1:56113-56132 MS.gene27117:CDS 50.0%
GACGCTTGGCAATGATGGCT+TGG - chr1.1:56249-56268 MS.gene27117:CDS 55.0%
! CTTCACACAAGCGGCACTGA+CGG - chr1.1:56280-56299 MS.gene27117:CDS 55.0%
!! TTCACACAAGCGGCACTGAC+GGG - chr1.1:56281-56300 MS.gene27117:CDS 55.0%
!! CAAGCGGCACTGACGGGTAA+AGG - chr1.1:56287-56306 MS.gene27117:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 56093 56394 56093 ID=MS.gene27117
chr1.1 mRNA 56093 56394 56093 ID=MS.gene27117.t1;Parent=MS.gene27117
chr1.1 exon 56093 56394 56093 ID=MS.gene27117.t1.exon1;Parent=MS.gene27117.t1
chr1.1 CDS 56093 56394 56093 ID=cds.MS.gene27117.t1;Parent=MS.gene27117.t1
Gene Sequence

>MS.gene27117

AATACGCCAGATATTGTGGGATTGAAGCACTGGAGCCTCTAGGAATATATCTGCCTGGTGATATAAACTACCCTGGAGGAGCATTGTTTGATCCCTTAAATCTGTCCAAAGATCCTGAAGCTTTTGAAGAGCTGAAAGTGAAAGAAATAAAAAATGGACGCTTGGCAATGATGGCTTGGTTAGGTTTCTTCACACAAGCGGCACTGACGGGTAAAGGTCCTGTACAAAACATTGTGGATCATATCTCCGATCCTTTCCACAACAACTTGCTTGGGTCACTCAATTCAATGAAATTACACTAG

Protein sequence

>MS.gene27117.t1

NTPDIVGLKHWSLUEYICLVIUTTLEEHCLIPUICPKILKLLKSUKUKKUKMDAWQUWLGUVSSHKRHURVKVLYKTLWIISPILSTTTCLGHSIQUNYT