AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene59935


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 19 sgRNAs with CRISPR-Local

Find 95 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAAATTATGCATACCTTTC+TGG 0.079710 8.2:-71610768 None:intergenic
CCTGGAGTTCTTGAGTTAAT+AGG 0.246781 8.2:+71609800 MS.gene59935:CDS
GATAGACTTCCTTTGATTCC+TGG 0.345188 8.2:+71609782 MS.gene59935:CDS
GTTCTTGAGTTAATAGGAAT+TGG 0.346748 8.2:+71609806 MS.gene59935:CDS
AAAGATTGCTCGTGATGTTA+TGG 0.420998 8.2:+71609384 MS.gene59935:CDS
TAGCTAGTGTATTTGCACTA+TGG 0.434144 8.2:+71609630 MS.gene59935:CDS
GATGTTATGGTAATGGCAAC+AGG 0.459632 8.2:+71609397 MS.gene59935:CDS
TTTGCACTATGGAGCTCAGC+TGG 0.465802 8.2:+71609641 MS.gene59935:CDS
TGAAGATTCTTAACAAACTC+AGG 0.486308 8.2:-71609454 None:intergenic
TCAGTTGAGTCAACTTCTGT+TGG 0.503744 8.2:-71609427 None:intergenic
TAAATATGCAGTGGGTTCAT+TGG 0.505381 8.2:+71609604 MS.gene59935:CDS
CCTATTAACTCAAGAACTCC+AGG 0.507872 8.2:-71609800 None:intergenic
TTAATAGGAATTGGTTACAC+TGG 0.524822 8.2:+71609815 MS.gene59935:CDS
TAATAGGAATTGGTTACACT+GGG 0.540472 8.2:+71609816 MS.gene59935:CDS
TCAAGAACTCCAGGAATCAA+AGG 0.544788 8.2:-71609791 None:intergenic
TGCTCGTGATGTTATGGTAA+TGG 0.557462 8.2:+71609390 MS.gene59935:CDS
TTTGATGATAAATATGCAGT+GGG 0.562064 8.2:+71609596 MS.gene59935:CDS
ATTTGATGATAAATATGCAG+TGG 0.570116 8.2:+71609595 MS.gene59935:CDS
AATAGGAATTGGTTACACTG+GGG 0.760636 8.2:+71609817 MS.gene59935:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAAATAACATAATTCT+AGG - chr8.2:71609554-71609573 None:intergenic 10.0%
!! ATAAAAATGTATTACAATAT+CGG - chr8.2:71610289-71610308 None:intergenic 10.0%
!! TATTTCAAAAAAAAAATACA+AGG - chr8.2:71609915-71609934 None:intergenic 10.0%
!! TGTATATATATATATATATG+AGG + chr8.2:71610649-71610668 MS.gene59935:intron 10.0%
!!! AATTATGTTATTTATTTTGT+TGG + chr8.2:71609556-71609575 MS.gene59935:intron 10.0%
! GGGGGGGGGGGGGGGGGGGG+GGG - chr8.2:71610176-71610195 None:intergenic 100.0%
!! TCAATAATAAACATTAAGAA+GGG - chr8.2:71609531-71609550 None:intergenic 15.0%
!!! TATTTTGTCATAAAATCAAA+TGG - chr8.2:71610466-71610485 None:intergenic 15.0%
!! AAATATAGAAGATGAATACT+AGG - chr8.2:71610708-71610727 None:intergenic 20.0%
!! CTTAGATTTAAATATATGAC+TGG + chr8.2:71609857-71609876 MS.gene59935:intron 20.0%
!! GTCAATAATAAACATTAAGA+AGG - chr8.2:71609532-71609551 None:intergenic 20.0%
!! TCTAATCTTATCAATTTGTA+AGG - chr8.2:71610329-71610348 None:intergenic 20.0%
!! TTACAAATTGATAAGATTAG+AGG + chr8.2:71610328-71610347 MS.gene59935:intron 20.0%
!!! TTTTTTTCCAGTAATTTCTA+GGG + chr8.2:71610406-71610425 MS.gene59935:intron 20.0%
! TAGGTTGTTAGAAATTGTAA+TGG + chr8.2:71609494-71609513 MS.gene59935:intron 25.0%
!! ATTTGATGATAAATATGCAG+TGG + chr8.2:71609595-71609614 MS.gene59935:CDS 25.0%
!! TATGAGGTATTATTCATCTT+TGG + chr8.2:71610665-71610684 MS.gene59935:intron 25.0%
!! TTTGATGATAAATATGCAGT+GGG + chr8.2:71609596-71609615 MS.gene59935:CDS 25.0%
!!! AATATCGCGTATTTGATTTT+TGG + chr8.2:71609888-71609907 MS.gene59935:intron 25.0%
!!! GTTTTTTTCCAGTAATTTCT+AGG + chr8.2:71610405-71610424 MS.gene59935:intron 25.0%
!!! TTGTTTTTTAAAATGATCCC+CGG + chr8.2:71609952-71609971 MS.gene59935:intron 25.0%
AAGAAAACCCTAGAAATTAC+TGG - chr8.2:71610416-71610435 None:intergenic 30.0%
AAGCACAATAGTATTTGACA+TGG - chr8.2:71610201-71610220 None:intergenic 30.0%
AATATCGGACTCTATCTATA+AGG - chr8.2:71610274-71610293 None:intergenic 30.0%
AGCACAATAGTATTTGACAT+GGG - chr8.2:71610200-71610219 None:intergenic 30.0%
ATCAAATGGCTTTCTTAAGA+TGG - chr8.2:71610452-71610471 None:intergenic 30.0%
ATGTCAAATACTATTGTGCT+TGG + chr8.2:71610200-71610219 MS.gene59935:intron 30.0%
CAATTACTTTCACTTGTAAC+AGG + chr8.2:71609754-71609773 MS.gene59935:intron 30.0%
GCATCAAGTGAAATAATGTT+GGG - chr8.2:71610087-71610106 None:intergenic 30.0%
GCTTTCTTAAGATGGTATTT+AGG - chr8.2:71610444-71610463 None:intergenic 30.0%
GTTCTTGAGTTAATAGGAAT+TGG + chr8.2:71609806-71609825 MS.gene59935:CDS 30.0%
TAATAGGAATTGGTTACACT+GGG + chr8.2:71609816-71609835 MS.gene59935:CDS 30.0%
TGAAGATTCTTAACAAACTC+AGG - chr8.2:71609457-71609476 None:intergenic 30.0%
TGCATCAAGTGAAATAATGT+TGG - chr8.2:71610088-71610107 None:intergenic 30.0%
TTAATAGGAATTGGTTACAC+TGG + chr8.2:71609815-71609834 MS.gene59935:CDS 30.0%
! ACTTGATGCATTGATATACA+TGG + chr8.2:71610098-71610117 MS.gene59935:intron 30.0%
! TATTTTGTTGGCATCATAGT+GGG + chr8.2:71609568-71609587 MS.gene59935:intron 30.0%
!! TTATTTTGTTGGCATCATAG+TGG + chr8.2:71609567-71609586 MS.gene59935:intron 30.0%
!!! TGTTTTTTAAAATGATCCCC+GGG + chr8.2:71609953-71609972 MS.gene59935:intron 30.0%
AAAGATTGCTCGTGATGTTA+TGG + chr8.2:71609384-71609403 MS.gene59935:CDS 35.0%
AATAGGAATTGGTTACACTG+GGG + chr8.2:71609817-71609836 MS.gene59935:CDS 35.0%
ACAATAGTATTTGACATGGG+GGG - chr8.2:71610197-71610216 None:intergenic 35.0%
ATCAAATACGCGATATTTGC+AGG - chr8.2:71609885-71609904 None:intergenic 35.0%
ATTGACATATAGATAGGGGA+TGG + chr8.2:71610226-71610245 MS.gene59935:intron 35.0%
CAAGAAGCTGTATGATACAT+AGG + chr8.2:71609475-71609494 MS.gene59935:intron 35.0%
CACAATAGTATTTGACATGG+GGG - chr8.2:71610198-71610217 None:intergenic 35.0%
CCTGCATTGAATAGTGTATA+AGG - chr8.2:71610136-71610155 None:intergenic 35.0%
CCTTATACACTATTCAATGC+AGG + chr8.2:71610133-71610152 MS.gene59935:intron 35.0%
GCACAATAGTATTTGACATG+GGG - chr8.2:71610199-71610218 None:intergenic 35.0%
TAGCTAGTGTATTTGCACTA+TGG + chr8.2:71609630-71609649 MS.gene59935:CDS 35.0%
TCAAATACGCGATATTTGCA+GGG - chr8.2:71609884-71609903 None:intergenic 35.0%
TTCTATATTTCACCTTGCAG+TGG + chr8.2:71610718-71610737 MS.gene59935:intron 35.0%
! GCATTGATATACATGGTTGA+TGG + chr8.2:71610105-71610124 MS.gene59935:intron 35.0%
! GGTGCATTGACATATAGATA+GGG + chr8.2:71610221-71610240 MS.gene59935:intron 35.0%
! GTGCATTGACATATAGATAG+GGG + chr8.2:71610222-71610241 MS.gene59935:intron 35.0%
! TAAATATGCAGTGGGTTCAT+TGG + chr8.2:71609604-71609623 MS.gene59935:CDS 35.0%
! TGGTGCATTGACATATAGAT+AGG + chr8.2:71610220-71610239 MS.gene59935:intron 35.0%
!! GATGGCCTTATAGAAGATAT+GGG + chr8.2:71610244-71610263 MS.gene59935:intron 35.0%
AATAGTATTTGACATGGGGG+GGG - chr8.2:71610195-71610214 None:intergenic 40.0%
ACACTATTCAATGCAGGACT+TGG + chr8.2:71610139-71610158 MS.gene59935:intron 40.0%
CAATAGTATTTGACATGGGG+GGG - chr8.2:71610196-71610215 None:intergenic 40.0%
CCTATTAACTCAAGAACTCC+AGG - chr8.2:71609803-71609822 None:intergenic 40.0%
CCTGGAGTTCTTGAGTTAAT+AGG + chr8.2:71609800-71609819 MS.gene59935:CDS 40.0%
GATAGACTTCCTTTGATTCC+TGG + chr8.2:71609782-71609801 MS.gene59935:CDS 40.0%
GCCATCCCATATCTTCTATA+AGG - chr8.2:71610252-71610271 None:intergenic 40.0%
GCCTTATAGAAGATATGGGA+TGG + chr8.2:71610248-71610267 MS.gene59935:intron 40.0%
TCAAGAACTCCAGGAATCAA+AGG - chr8.2:71609794-71609813 None:intergenic 40.0%
TCAGTTGAGTCAACTTCTGT+TGG - chr8.2:71609430-71609449 None:intergenic 40.0%
TGCTCGTGATGTTATGGTAA+TGG + chr8.2:71609390-71609409 MS.gene59935:CDS 40.0%
!! GATATACATGGTTGATGGTC+AGG + chr8.2:71610110-71610129 MS.gene59935:intron 40.0%
!! GATGTTATGGTAATGGCAAC+AGG + chr8.2:71609397-71609416 MS.gene59935:CDS 40.0%
!! GGATGGCCTTATAGAAGATA+TGG + chr8.2:71610243-71610262 MS.gene59935:intron 40.0%
ATAGTATTTGACATGGGGGG+GGG - chr8.2:71610194-71610213 None:intergenic 45.0%
CCTATCAGCTTAAATGGACC+CGG - chr8.2:71609974-71609993 None:intergenic 45.0%
CTATCAGCTTAAATGGACCC+GGG - chr8.2:71609973-71609992 None:intergenic 45.0%
CTGCCACCTATCAGCTTAAA+TGG - chr8.2:71609980-71609999 None:intergenic 45.0%
CTTGTATGCAAACCACTGCA+AGG - chr8.2:71610733-71610752 None:intergenic 45.0%
GGTCCATTTAAGCTGATAGG+TGG + chr8.2:71609974-71609993 MS.gene59935:intron 45.0%
TATCAGCTTAAATGGACCCG+GGG - chr8.2:71609972-71609991 None:intergenic 45.0%
!!! GAGCTCAGCTGGTTTTATCT+CGG + chr8.2:71609652-71609671 MS.gene59935:CDS 45.0%
CCGGGTCCATTTAAGCTGAT+AGG + chr8.2:71609971-71609990 MS.gene59935:intron 50.0%
TAGTATTTGACATGGGGGGG+GGG - chr8.2:71610193-71610212 None:intergenic 50.0%
TTTGCACTATGGAGCTCAGC+TGG + chr8.2:71609641-71609660 MS.gene59935:CDS 50.0%
AGTATTTGACATGGGGGGGG+GGG - chr8.2:71610192-71610211 None:intergenic 55.0%
GTATTTGACATGGGGGGGGG+GGG - chr8.2:71610191-71610210 None:intergenic 60.0%
TATTTGACATGGGGGGGGGG+GGG - chr8.2:71610190-71610209 None:intergenic 60.0%
ATTTGACATGGGGGGGGGGG+GGG - chr8.2:71610189-71610208 None:intergenic 65.0%
TTTGACATGGGGGGGGGGGG+GGG - chr8.2:71610188-71610207 None:intergenic 70.0%
TTGACATGGGGGGGGGGGGG+GGG - chr8.2:71610187-71610206 None:intergenic 75.0%
! TGACATGGGGGGGGGGGGGG+GGG - chr8.2:71610186-71610205 None:intergenic 80.0%
! ACATGGGGGGGGGGGGGGGG+GGG - chr8.2:71610184-71610203 None:intergenic 85.0%
! GACATGGGGGGGGGGGGGGG+GGG - chr8.2:71610185-71610204 None:intergenic 85.0%
! ATGGGGGGGGGGGGGGGGGG+GGG - chr8.2:71610182-71610201 None:intergenic 90.0%
! CATGGGGGGGGGGGGGGGGG+GGG - chr8.2:71610183-71610202 None:intergenic 90.0%
! TGGGGGGGGGGGGGGGGGGG+GGG - chr8.2:71610181-71610200 None:intergenic 95.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 71609380 71610775 71609380 ID=MS.gene59935
chr8.2 mRNA 71609380 71610775 71609380 ID=MS.gene59935.t1;Parent=MS.gene59935
chr8.2 exon 71609380 71609483 71609380 ID=MS.gene59935.t1.exon1;Parent=MS.gene59935.t1
chr8.2 CDS 71609380 71609483 71609380 ID=cds.MS.gene59935.t1;Parent=MS.gene59935.t1
chr8.2 exon 71609587 71609673 71609587 ID=MS.gene59935.t1.exon2;Parent=MS.gene59935.t1
chr8.2 CDS 71609587 71609673 71609587 ID=cds.MS.gene59935.t1;Parent=MS.gene59935.t1
chr8.2 exon 71609776 71609838 71609776 ID=MS.gene59935.t1.exon3;Parent=MS.gene59935.t1
chr8.2 CDS 71609776 71609838 71609776 ID=cds.MS.gene59935.t1;Parent=MS.gene59935.t1
chr8.2 exon 71610738 71610775 71610738 ID=MS.gene59935.t1.exon4;Parent=MS.gene59935.t1
chr8.2 CDS 71610738 71610775 71610738 ID=cds.MS.gene59935.t1;Parent=MS.gene59935.t1
Gene Sequence

>MS.gene59935

ATCGAAAGATTGCTCGTGATGTTATGGTAATGGCAACAGGAGATGCACCAACAGAAGTTGACTCAACTGAGCTACCTGAGTTTGTTAAGAATCTTCAAGAAGCTGTATGATACATAGGTTGTTAGAAATTGTAATGGAGTTTCTTTTTCCCTTCTTAATGTTTATTATTGACCTAGAATTATGTTATTTATTTTGTTGGCATCATAGTGGGACAAATTTGATGATAAATATGCAGTGGGTTCATTGGTTGTAGCTAGTGTATTTGCACTATGGAGCTCAGCTGGTTTTATCTCGGTATTAAGTTATTTAAATGCTAATTTTATTTGCATTATTTGATTATCAATTTATTAGATACAGATGATAAATGTCATAAGCAATTACTTTCACTTGTAACAGGCAATTGATAGACTTCCTTTGATTCCTGGAGTTCTTGAGTTAATAGGAATTGGTTACACTGGGGTAAGTCAAATAAATACACTTAGATTTAAATATATGACTGGTCCCTGCAAATATCGCGTATTTGATTTTTGGTCCTTGTATTTTTTTTTTGAAATACATCTTTGCAAGAAAAATTGTTTTTTAAAATGATCCCCGGGTCCATTTAAGCTGATAGGTGGCAGTCTGATGCTTACGTTACACATGCTAAAAAAAATAAAATCTGAAACTTAATTTAAAAAAAAAATATAAATAGATTTTTTCATATGCCCAACATTATTTCACTTGATGCATTGATATACATGGTTGATGGTCAGGCCTTATACACTATTCAATGCAGGACTTGGATTTTTGTTTTACACCCCCCCCCCCCCCCCCCCCCCCCATGTCAAATACTATTGTGCTTGGTGCATTGACATATAGATAGGGGATGGCCTTATAGAAGATATGGGATGGCCTTATAGATAGAGTCCGATATTGTAATACATTTTTATATTTAGACATAACTCATCCTTACAAATTGATAAGATTAGAGGTGTCACTCTTTATAAACTCATCTCAAGCATTATATTTATTCAATGCGACACTTAGTTTTTTTCCAGTAATTTCTAGGGTTTTCTTTTAATCCTAAATACCATCTTAAGAAAGCCATTTGATTTTATGACAAAATACATATATACTAGCAAAAACAATATATATATATATATATATATATGTATATATATCTATATATATATATATATATATATGTATATATATATCTATATATATGTATATATATATATATATATGTATATATATATATATATATATATATGTGTGTATATATATATATGTATATATATATATATATGAGGTATTATTCATCTTTGGATTCAACTATAAGAAAACCTAGTATTCATCTTCTATATTTCACCTTGCAGTGGTTTGCATACAAGAACGTAGTTTTCAAGCCAGAAAG

Protein sequence

>MS.gene59935.t1

IERLLVMLWUWQQEMHQQKLTQLSYLSLLRIFKKLGTNLMINMQWVHWLULVYLHYGAQLVLSRQLIDFLUFLEFLSUUELVTLGGLHTRTUFSSQK