AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene87268


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87268.t1 MTR_2g082580 98.696 230 3 0 1 230 1 230 1.17e-170 469
MS.gene87268.t1 MTR_5g041910 29.358 109 77 0 104 212 141 249 1.39e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87268.t1 AT3G01440 55.357 224 94 3 1 223 1 219 4.14e-83 247
MS.gene87268.t1 AT4G21280 25.532 235 150 4 1 223 1 222 1.37e-18 81.6
MS.gene87268.t1 AT4G21280 25.424 236 150 5 1 223 1 223 7.32e-18 79.7
MS.gene87268.t1 AT4G05180 27.684 177 124 2 50 223 54 229 1.40e-14 70.9
MS.gene87268.t1 AT4G05180 27.684 177 124 2 50 223 54 229 1.40e-14 70.9
MS.gene87268.t1 AT1G14150 28.346 127 91 0 95 221 61 187 4.33e-13 65.9
MS.gene87268.t1 AT1G14150 30.303 99 69 0 95 193 61 159 2.72e-11 60.5

Find 38 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACTACTTGTCCTGTCTTAA+AGG 0.141076 2.2:-14439521 None:intergenic
TAGCTAAGGATAATGGATTT+TGG 0.177277 2.2:+14439671 MS.gene87268:CDS
CAAGTAGTAGTTGGATTTCT+TGG 0.273988 2.2:+14439535 MS.gene87268:CDS
TTAAAGGTTCTTTGTGCATT+TGG 0.275452 2.2:-14439505 None:intergenic
TTTGGAACAAGGCATGTAAT+TGG 0.297323 2.2:-14439487 None:intergenic
CTATTAGTGACAGTTGGTTC+TGG 0.310416 2.2:-14439709 None:intergenic
AAACTCTACATGGCAAATAT+TGG 0.316818 2.2:+14440717 MS.gene87268:CDS
GTGACAGTTGGTTCTGGAAT+AGG 0.317646 2.2:-14439703 None:intergenic
ATTTCACTAGCTAAGGATAA+TGG 0.333626 2.2:+14439664 MS.gene87268:CDS
CTTTGTTTCTTTAGTTCTTA+CGG 0.338232 2.2:+14439624 MS.gene87268:CDS
TGAAAGTAGACTTGAGTCTT+AGG 0.346421 2.2:-14440839 None:intergenic
TGTAATTGGAGTTAAAGTTT+TGG 0.348907 2.2:-14439473 None:intergenic
TTTGTTTCTTTAGTTCTTAC+GGG 0.353615 2.2:+14439625 MS.gene87268:CDS
GATCCTTAAAACACTTCCTT+TGG 0.359342 2.2:-14440742 None:intergenic
GATATTGCAAATGAGAAAAC+GGG 0.400999 2.2:+14440672 MS.gene87268:intron
TGAAACACAAGCATGCTATA+AGG 0.409707 2.2:+14441258 MS.gene87268:CDS
AATTGCTGCTTATCTTCAGC+TGG 0.415937 2.2:-14440897 None:intergenic
ACTCCATGCAAATTTGTTAG+AGG 0.418986 2.2:-14439448 None:intergenic
AAGACACACTCAACTATGTT+AGG 0.422431 2.2:+14440811 MS.gene87268:CDS
TGCAGCTTGAAGAAGCTTCA+AGG 0.422761 2.2:+14441221 MS.gene87268:intron
AGATATTGCAAATGAGAAAA+CGG 0.438542 2.2:+14440671 MS.gene87268:intron
AAGACAGGACAAGTAGTAGT+TGG 0.458371 2.2:+14439526 MS.gene87268:CDS
ATTGTTTGATAACTTTGAAA+AGG 0.460958 2.2:+14440938 MS.gene87268:CDS
AGCTTACTATTAGTGACAGT+TGG 0.481220 2.2:-14439715 None:intergenic
TTCTTTGTGCATTTGGAACA+AGG 0.495526 2.2:-14439498 None:intergenic
TGCATTTGATCTTCTTGCAA+TGG 0.504301 2.2:+14440773 MS.gene87268:CDS
TGAGAAGATTTCACTAGCTA+AGG 0.517657 2.2:+14439657 MS.gene87268:CDS
TGCTTAAAGATGTCATGGAC+AGG 0.522204 2.2:+14441293 MS.gene87268:CDS
ATTTGATCTTCTTGCAATGG+CGG 0.551344 2.2:+14440776 MS.gene87268:CDS
ATTGCTGCTTATCTTCAGCT+GGG 0.551798 2.2:-14440896 None:intergenic
ATGGCAAATATTGGAGCCAA+AGG 0.555680 2.2:+14440726 MS.gene87268:CDS
GCACAAAGAACCTTTAAGAC+AGG 0.560597 2.2:+14439511 MS.gene87268:CDS
TAAGCAGCAATTAACTGATG+TGG 0.578245 2.2:+14440908 MS.gene87268:CDS
AGTTATGCTTAAAGATGTCA+TGG 0.580022 2.2:+14441288 MS.gene87268:CDS
GCTCCTCTAACAAATTTGCA+TGG 0.591211 2.2:+14439445 MS.gene87268:CDS
TATCAAGAAGAAACTCTACA+TGG 0.592066 2.2:+14440707 MS.gene87268:CDS
ACACACTCAACTATGTTAGG+AGG 0.598523 2.2:+14440814 MS.gene87268:CDS
ATATTGCAAATGAGAAAACG+GGG 0.743479 2.2:+14440673 MS.gene87268:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CGATTTAATAAAAAAAAAAT+AGG - chr2.2:14440353-14440372 None:intergenic 10.0%
!! TTAATAATAAATAAATGCAA+TGG + chr2.2:14439999-14440018 MS.gene87268:intron 10.0%
!!! ATGAAATTTTGAATATTTAT+AGG - chr2.2:14440122-14440141 None:intergenic 10.0%
!!! TGAAATTTTGAATATTTATA+GGG - chr2.2:14440121-14440140 None:intergenic 10.0%
!!! TTTTTTTTTTATTAAATCGA+TGG + chr2.2:14440354-14440373 MS.gene87268:intron 10.0%
!! AAATATTAATTGTACTATCA+TGG + chr2.2:14439952-14439971 MS.gene87268:intron 15.0%
!! ACAAAATTCTGAATATTTAT+AGG + chr2.2:14440308-14440327 MS.gene87268:intron 15.0%
!! ATTCACATAATATTAAATTG+CGG - chr2.2:14441166-14441185 None:intergenic 15.0%
!! ATTCTATTATATTTAAGCTT+AGG - chr2.2:14440511-14440530 None:intergenic 15.0%
!! CAAAATTCTGAATATTTATA+GGG + chr2.2:14440309-14440328 MS.gene87268:intron 15.0%
!! TATAGCTAATTAATTTACAT+AGG + chr2.2:14439748-14439767 MS.gene87268:intron 15.0%
!!! AAAGTGTGATTTTATTTTAT+AGG - chr2.2:14440153-14440172 None:intergenic 15.0%
!!! AAGTGTGATTTTATTTTATA+GGG - chr2.2:14440152-14440171 None:intergenic 15.0%
!!! AATCTTGATCATTATATTTT+TGG + chr2.2:14440077-14440096 MS.gene87268:intron 15.0%
!!! ATCTTGATCATTATATTTTT+GGG + chr2.2:14440078-14440097 MS.gene87268:intron 15.0%
!! AATATTATGTGAATAGATGT+TGG + chr2.2:14441172-14441191 MS.gene87268:intron 20.0%
!! CAATGTAAATGATGATTATA+TGG + chr2.2:14440646-14440665 MS.gene87268:intron 20.0%
!! TTACTTTAAATGTTAGAGTA+TGG - chr2.2:14440474-14440493 None:intergenic 20.0%
!! TTTATGTTTCAATTGACATT+TGG + chr2.2:14439840-14439859 MS.gene87268:intron 20.0%
!!! ATATTTTCCTTTAGCATTTT+AGG - chr2.2:14440245-14440264 None:intergenic 20.0%
!!! ATTGTTTGATAACTTTGAAA+AGG + chr2.2:14440938-14440957 MS.gene87268:CDS 20.0%
!!! TATTTTCCTTTAGCATTTTA+GGG - chr2.2:14440244-14440263 None:intergenic 20.0%
!!! TTTTGGGTTAATTAAGTAAA+TGG + chr2.2:14440094-14440113 MS.gene87268:intron 20.0%
! AGATATTGCAAATGAGAAAA+CGG + chr2.2:14440671-14440690 MS.gene87268:intron 25.0%
! CATAACTGTATAAAAGAACA+TGG - chr2.2:14439900-14439919 None:intergenic 25.0%
! TAGTCCCTGTAAAAAATTTA+CGG + chr2.2:14440205-14440224 MS.gene87268:intron 25.0%
! TATAGTTAACTTCATCATGA+TGG + chr2.2:14440378-14440397 MS.gene87268:intron 25.0%
!! ACATTTGGAACAATTATGAT+TGG + chr2.2:14439855-14439874 MS.gene87268:intron 25.0%
!! TGTAATTGGAGTTAAAGTTT+TGG - chr2.2:14439476-14439495 None:intergenic 25.0%
!!! AAAGTGTGACTTTAATTTGT+AGG + chr2.2:14440277-14440296 MS.gene87268:intron 25.0%
!!! AAGTGTGACTTTAATTTGTA+GGG + chr2.2:14440278-14440297 MS.gene87268:intron 25.0%
!!! ATCTGTTTACTTTTGAACTA+GGG + chr2.2:14440611-14440630 MS.gene87268:intron 25.0%
!!! CTTTGTTTCTTTAGTTCTTA+CGG + chr2.2:14439624-14439643 MS.gene87268:CDS 25.0%
!!! GTATGCTCTTTTTTAAATTG+CGG + chr2.2:14440960-14440979 MS.gene87268:intron 25.0%
!!! TATCTGTTTACTTTTGAACT+AGG + chr2.2:14440610-14440629 MS.gene87268:intron 25.0%
!!! TTTGTTTCTTTAGTTCTTAC+GGG + chr2.2:14439625-14439644 MS.gene87268:CDS 25.0%
AAACTCTACATGGCAAATAT+TGG + chr2.2:14440717-14440736 MS.gene87268:CDS 30.0%
AGTTATGCTTAAAGATGTCA+TGG + chr2.2:14441288-14441307 MS.gene87268:CDS 30.0%
ATATTGCAAATGAGAAAACG+GGG + chr2.2:14440673-14440692 MS.gene87268:CDS 30.0%
ATGCTAAAGGAAAATATCAC+AGG + chr2.2:14440248-14440267 MS.gene87268:intron 30.0%
ATTTCACTAGCTAAGGATAA+TGG + chr2.2:14439664-14439683 MS.gene87268:CDS 30.0%
CAAAATATTGCAGACAAATG+TGG + chr2.2:14441011-14441030 MS.gene87268:intron 30.0%
CATAAAGTTGCAAACAAATG+TGG + chr2.2:14441084-14441103 MS.gene87268:intron 30.0%
CTTATGATTGAAAGTTAGCA+TGG + chr2.2:14439921-14439940 MS.gene87268:intron 30.0%
GATATTGCAAATGAGAAAAC+GGG + chr2.2:14440672-14440691 MS.gene87268:intron 30.0%
TATCAAGAAGAAACTCTACA+TGG + chr2.2:14440707-14440726 MS.gene87268:CDS 30.0%
TGCTAAAGGAAAATATCACA+GGG + chr2.2:14440249-14440268 MS.gene87268:intron 30.0%
TTAAAGGTTCTTTGTGCATT+TGG - chr2.2:14439508-14439527 None:intergenic 30.0%
! AAAATTTACGGCAGCATTTT+CGG + chr2.2:14440217-14440236 MS.gene87268:intron 30.0%
! ATGTTGACGGAAAATTTTAC+AGG - chr2.2:14440182-14440201 None:intergenic 30.0%
! CAATATTTTATCGCACAGTT+CGG - chr2.2:14440566-14440585 None:intergenic 30.0%
! CACATTTGTCTGCAATATTT+TGG - chr2.2:14441013-14441032 None:intergenic 30.0%
! TGTTGACGGAAAATTTTACA+GGG - chr2.2:14440181-14440200 None:intergenic 30.0%
!! CATGTACTTTGATTTTGACA+AGG + chr2.2:14440860-14440879 MS.gene87268:CDS 30.0%
!!! GATAATGGATTTTGGATTGA+TGG + chr2.2:14439679-14439698 MS.gene87268:CDS 30.0%
!!! TAGCTAAGGATAATGGATTT+TGG + chr2.2:14439671-14439690 MS.gene87268:CDS 30.0%
AAGACACACTCAACTATGTT+AGG + chr2.2:14440811-14440830 MS.gene87268:CDS 35.0%
ACAGGGACTAAAAATGTTGA+CGG - chr2.2:14440195-14440214 None:intergenic 35.0%
AGCTTACTATTAGTGACAGT+TGG - chr2.2:14439718-14439737 None:intergenic 35.0%
ATTTGATCTTCTTGCAATGG+CGG + chr2.2:14440776-14440795 MS.gene87268:CDS 35.0%
CAAGTAGTAGTTGGATTTCT+TGG + chr2.2:14439535-14439554 MS.gene87268:CDS 35.0%
GACCTATTAGTGAAGTACAA+TGG + chr2.2:14440040-14440059 MS.gene87268:intron 35.0%
GATCCTTAAAACACTTCCTT+TGG - chr2.2:14440745-14440764 None:intergenic 35.0%
TAAGCAGCAATTAACTGATG+TGG + chr2.2:14440908-14440927 MS.gene87268:CDS 35.0%
TACTACTTGTCCTGTCTTAA+AGG - chr2.2:14439524-14439543 None:intergenic 35.0%
TGAAACACAAGCATGCTATA+AGG + chr2.2:14441258-14441277 MS.gene87268:CDS 35.0%
TGAAAGTAGACTTGAGTCTT+AGG - chr2.2:14440842-14440861 None:intergenic 35.0%
TGAGAAGATTTCACTAGCTA+AGG + chr2.2:14439657-14439676 MS.gene87268:CDS 35.0%
TGCATTTGATCTTCTTGCAA+TGG + chr2.2:14440773-14440792 MS.gene87268:CDS 35.0%
TGCCATTGTACTTCACTAAT+AGG - chr2.2:14440045-14440064 None:intergenic 35.0%
TTTAAATTGCGGTTACGTAG+TGG + chr2.2:14440971-14440990 MS.gene87268:intron 35.0%
! TTCTTTGTGCATTTGGAACA+AGG - chr2.2:14439501-14439520 None:intergenic 35.0%
! TTTGGAACAAGGCATGTAAT+TGG - chr2.2:14439490-14439509 None:intergenic 35.0%
!! ACTCCATGCAAATTTGTTAG+AGG - chr2.2:14439451-14439470 None:intergenic 35.0%
!!! GCTGCCGTAAATTTTTTACA+GGG - chr2.2:14440212-14440231 None:intergenic 35.0%
!!! TGCTGCCGTAAATTTTTTAC+AGG - chr2.2:14440213-14440232 None:intergenic 35.0%
AAGACAGGACAAGTAGTAGT+TGG + chr2.2:14439526-14439545 MS.gene87268:CDS 40.0%
ACACACTCAACTATGTTAGG+AGG + chr2.2:14440814-14440833 MS.gene87268:CDS 40.0%
ATGGCAAATATTGGAGCCAA+AGG + chr2.2:14440726-14440745 MS.gene87268:CDS 40.0%
GACTTGTGCACTTATCAGTA+AGG - chr2.2:14440435-14440454 None:intergenic 40.0%
GCTCCTCTAACAAATTTGCA+TGG + chr2.2:14439445-14439464 MS.gene87268:CDS 40.0%
TGCTTAAAGATGTCATGGAC+AGG + chr2.2:14441293-14441312 MS.gene87268:CDS 40.0%
TTCGGTCCCTAAAATGCTAA+AGG + chr2.2:14440235-14440254 MS.gene87268:intron 40.0%
! AATTGCTGCTTATCTTCAGC+TGG - chr2.2:14440900-14440919 None:intergenic 40.0%
! ATTGCTGCTTATCTTCAGCT+GGG - chr2.2:14440899-14440918 None:intergenic 40.0%
! CTATTAGTGACAGTTGGTTC+TGG - chr2.2:14439712-14439731 None:intergenic 40.0%
! TTGCGGTCATGTTTGTGTTT+TGG + chr2.2:14441049-14441068 MS.gene87268:intron 40.0%
!! GCACAAAGAACCTTTAAGAC+AGG + chr2.2:14439511-14439530 MS.gene87268:CDS 40.0%
!!! GAGCCAAAGGAAGTGTTTTA+AGG + chr2.2:14440739-14440758 MS.gene87268:CDS 40.0%
AATTGCGGCACAAATGTCGT+TGG - chr2.2:14441132-14441151 None:intergenic 45.0%
ATGTCGTTGGACCACAATTG+CGG - chr2.2:14441119-14441138 None:intergenic 45.0%
CACAAACATGACCGCAATTG+TGG - chr2.2:14441046-14441065 None:intergenic 45.0%
GTCAGAGACGTAGACACAAT+TGG + chr2.2:14440540-14440559 MS.gene87268:intron 45.0%
TGCAGCTTGAAGAAGCTTCA+AGG + chr2.2:14441221-14441240 MS.gene87268:intron 45.0%
!! GTGACAGTTGGTTCTGGAAT+AGG - chr2.2:14439706-14439725 None:intergenic 45.0%
!! GATTTAATAAAAAAAAAATA+GGG - chr2.2:14440352-14440371 None:intergenic 5.0%
GCGGTCAACAACTGCAATTG+CGG - chr2.2:14441147-14441166 None:intergenic 50.0%
! GGTTGATGCGACCACAATTG+CGG + chr2.2:14441032-14441051 MS.gene87268:intron 50.0%
GGCTAATGCAGCCGCAATTG+TGG + chr2.2:14441105-14441124 MS.gene87268:intron 55.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 14439442 14441342 14439442 ID=MS.gene87268
chr2.2 mRNA 14439442 14441342 14439442 ID=MS.gene87268.t1;Parent=MS.gene87268
chr2.2 exon 14439442 14439730 14439442 ID=MS.gene87268.t1.exon1;Parent=MS.gene87268.t1
chr2.2 CDS 14439442 14439730 14439442 ID=cds.MS.gene87268.t1;Parent=MS.gene87268.t1
chr2.2 exon 14440673 14440959 14440673 ID=MS.gene87268.t1.exon2;Parent=MS.gene87268.t1
chr2.2 CDS 14440673 14440959 14440673 ID=cds.MS.gene87268.t1;Parent=MS.gene87268.t1
chr2.2 exon 14441226 14441342 14441226 ID=MS.gene87268.t1.exon3;Parent=MS.gene87268.t1
chr2.2 CDS 14441226 14441342 14441226 ID=cds.MS.gene87268.t1;Parent=MS.gene87268.t1
Gene Sequence

>MS.gene87268

ATGGCTCCTCTAACAAATTTGCATGGAGTCTCCAAAACTTTAACTCCAATTACATGCCTTGTTCCAAATGCACAAAGAACCTTTAAGACAGGACAAGTAGTAGTTGGATTTCTTGGAAGCAAAACAAAGAAATCATCAGAATGTGAATCAGTTAACACTACAAGAAGAGCAGCTGCAATAAGCTTTGTTTCTTTAGTTCTTACGGGACAATTCAGTGAGAAGATTTCACTAGCTAAGGATAATGGATTTTGGATTGATGGACCTATTCCAGAACCAACTGTCACTAATAGTAAGCTTAATTTCTTGTATAGCTAATTAATTTACATAGGACAAAATGAATTGTATATAGTTCTTGCTTGCAAATACATGCATTTCATTGCTCTTATGTTTGTTTGATCTTTATGTTTCAATTGACATTTGGAACAATTATGATTGGTTTTTTGTTCTTTCTTTTACCATGTTCTTTTATACAGTTATGACTTATGATTGAAAGTTAGCATGGTGAACTTAAAATATTAATTGTACTATCATGGTGAAGTTAAAAAATAATTATTGATTTAATAATAAATAAATGCAATGGTCAAGATTTAATATGAGTGACCTATTAGTGAAGTACAATGGCAAGAAATATATTAAATCTTGATCATTATATTTTTGGGTTAATTAAGTAAATGGTCCCTATAAATATTCAAAATTTCATTTTTAGTCCCTATAAAATAAAATCACACTTTTTAGTCCCTGTAAAATTTTCCGTCAACATTTTTAGTCCCTGTAAAAAATTTACGGCAGCATTTTCGGTCCCTAAAATGCTAAAGGAAAATATCACAGGGACTAAAAAGTGTGACTTTAATTTGTAGGGACTAAAAACAAAATTCTGAATATTTATAGGGATCATTTACTTAATTAACCCTATTTTTTTTTTATTAAATCGATGGTTATAGTTAACTTCATCATGATGGTTCAATCTTAAGTTCACTATACTAATTGTCACCTTACTGATAAGTGCACAAGTCGCTTGAGCTGACTAGACCATACTCTAACATTTAAAGTAAGTTTGTAAAGTAATCCTAAGCTTAAATATAATAGAATATCGATATAGTCAGAGACGTAGACACAATTGGCCGAACTGTGCGATAAAATATTGTGTTTTCTTTGTTTGTGTTTTTCGTATCTGTTTACTTTTGAACTAGGGTTCTTGTTCTAACAATGTAAATGATGATTATATGGTTAGATATTGCAAATGAGAAAACGGGGACACGTTCTTTTATCAAGAAGAAACTCTACATGGCAAATATTGGAGCCAAAGGAAGTGTTTTAAGGATCAAGAAATATGCATTTGATCTTCTTGCAATGGCGGATTTGATTGCACAAGACACACTCAACTATGTTAGGAGGTACCTAAGACTCAAGTCTACTTTCATGTACTTTGATTTTGACAAGGTTATCTCTGCTGTCCCAGCTGAAGATAAGCAGCAATTAACTGATGTGGCAAACAAATTGTTTGATAACTTTGAAAAGGTATGCTCTTTTTTAAATTGCGGTTACGTAGTGGTCGATGTGATTGTAGTCCAAAATATTGCAGACAAATGTGGTTGATGCGACCACAATTGCGGTCATGTTTGTGTTTTGGTACTTGATGTTGCATAAAGTTGCAAACAAATGTGGCTAATGCAGCCGCAATTGTGGTCCAACGACATTTGTGCCGCAATTGCAGTTGTTGACCGCAATTTAATATTATGTGAATAGATGTTGGATTAATGAATTGAATTTTTTATGCAATGCAGCTTGAAGAAGCTTCAAGGAACAAAAATCTACTTGAAACACAAGCATGCTATAAGGAAACTGAAGTTATGCTTAAAGATGTCATGGACAGGATGACTATAATGTATAAAACAATTTGA

Protein sequence

>MS.gene87268.t1

MAPLTNLHGVSKTLTPITCLVPNAQRTFKTGQVVVGFLGSKTKKSSECESVNTTRRAAAISFVSLVLTGQFSEKISLAKDNGFWIDGPIPEPTVTNNIANEKTGTRSFIKKKLYMANIGAKGSVLRIKKYAFDLLAMADLIAQDTLNYVRRYLRLKSTFMYFDFDKVISAVPAEDKQQLTDVANKLFDNFEKLEEASRNKNLLETQACYKETEVMLKDVMDRMTIMYKTI