Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71558.t1 | XP_003622313.2 | 95.4 | 239 | 11 | 0 | 32 | 270 | 46 | 284 | 1.20E-126 | 462.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71558.t1 | G7L5H0 | 95.4 | 239 | 11 | 0 | 32 | 270 | 46 | 284 | 9.0e-127 | 462.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049149 | MS.gene71558 | 0.811746 | 6.00E-51 | -1.69E-46 |
| MS.gene049150 | MS.gene71558 | 0.819594 | 1.07E-52 | -1.69E-46 |
| MS.gene049153 | MS.gene71558 | 0.82352 | 1.33E-53 | -1.69E-46 |
| MS.gene049346 | MS.gene71558 | 0.884519 | 1.79E-71 | -1.69E-46 |
| MS.gene049351 | MS.gene71558 | 0.806622 | 7.52E-50 | -1.69E-46 |
| MS.gene049661 | MS.gene71558 | 0.833183 | 6.17E-56 | -1.69E-46 |
| MS.gene049662 | MS.gene71558 | 0.837483 | 5.06E-57 | -1.69E-46 |
| MS.gene049758 | MS.gene71558 | 0.846181 | 2.55E-59 | -1.69E-46 |
| MS.gene049910 | MS.gene71558 | 0.850144 | 2.05E-60 | -1.69E-46 |
| MS.gene049915 | MS.gene71558 | 0.827012 | 1.98E-54 | -1.69E-46 |
| MS.gene049993 | MS.gene71558 | 0.830645 | 2.61E-55 | -1.69E-46 |
| MS.gene050076 | MS.gene71558 | 0.815561 | 8.68E-52 | -1.69E-46 |
| MS.gene050301 | MS.gene71558 | 0.87127 | 7.77E-67 | -1.69E-46 |
| MS.gene050397 | MS.gene71558 | 0.875367 | 3.26E-68 | -1.69E-46 |
| MS.gene050400 | MS.gene71558 | 0.804832 | 1.79E-49 | -1.69E-46 |
| MS.gene050404 | MS.gene71558 | 0.838869 | 2.22E-57 | -1.69E-46 |
| MS.gene050406 | MS.gene71558 | 0.866797 | 2.19E-65 | -1.69E-46 |
| MS.gene050426 | MS.gene71558 | -0.854896 | 9.07E-62 | -1.69E-46 |
| MS.gene050432 | MS.gene71558 | 0.827183 | 1.80E-54 | -1.69E-46 |
| MS.gene050464 | MS.gene71558 | 0.86781 | 1.04E-65 | -1.69E-46 |
| MS.gene050529 | MS.gene71558 | 0.874253 | 7.81E-68 | -1.69E-46 |
| MS.gene050642 | MS.gene71558 | 0.82437 | 8.37E-54 | -1.69E-46 |
| MS.gene050670 | MS.gene71558 | 0.883201 | 5.48E-71 | -1.69E-46 |
| MS.gene050812 | MS.gene71558 | 0.817318 | 3.51E-52 | -1.69E-46 |
| MS.gene050820 | MS.gene71558 | 0.811641 | 6.33E-51 | -1.69E-46 |
| MS.gene05101 | MS.gene71558 | 0.836797 | 7.58E-57 | -1.69E-46 |
| MS.gene05105 | MS.gene71558 | 0.856278 | 3.59E-62 | -1.69E-46 |
| MS.gene051171 | MS.gene71558 | 0.804037 | 2.62E-49 | -1.69E-46 |
| MS.gene051208 | MS.gene71558 | 0.804752 | 1.86E-49 | -1.69E-46 |
| MS.gene051240 | MS.gene71558 | 0.816638 | 4.99E-52 | -1.69E-46 |
| MS.gene051242 | MS.gene71558 | 0.81531 | 9.87E-52 | -1.69E-46 |
| MS.gene051471 | MS.gene71558 | 0.803552 | 3.30E-49 | -1.69E-46 |
| MS.gene051525 | MS.gene71558 | 0.861934 | 7.23E-64 | -1.69E-46 |
| MS.gene051582 | MS.gene71558 | 0.889557 | 2.18E-73 | -1.69E-46 |
| MS.gene051860 | MS.gene71558 | 0.835893 | 1.29E-56 | -1.69E-46 |
| MS.gene051983 | MS.gene71558 | 0.826318 | 2.90E-54 | -1.69E-46 |
| MS.gene052011 | MS.gene71558 | -0.882975 | 6.63E-71 | -1.69E-46 |
| MS.gene052077 | MS.gene71558 | 0.872146 | 3.98E-67 | -1.69E-46 |
| MS.gene052078 | MS.gene71558 | 0.834651 | 2.65E-56 | -1.69E-46 |
| MS.gene052134 | MS.gene71558 | 0.861262 | 1.16E-63 | -1.69E-46 |
| MS.gene052135 | MS.gene71558 | 0.871961 | 4.58E-67 | -1.69E-46 |
| MS.gene052136 | MS.gene71558 | 0.826504 | 2.62E-54 | -1.69E-46 |
| MS.gene052178 | MS.gene71558 | 0.804761 | 1.85E-49 | -1.69E-46 |
| MS.gene052188 | MS.gene71558 | 0.873988 | 9.59E-68 | -1.69E-46 |
| MS.gene052253 | MS.gene71558 | 0.899096 | 2.78E-77 | -1.69E-46 |
| MS.gene052255 | MS.gene71558 | 0.823391 | 1.42E-53 | -1.69E-46 |
| MS.gene052310 | MS.gene71558 | 0.87004 | 1.97E-66 | -1.69E-46 |
| MS.gene052340 | MS.gene71558 | 0.888415 | 6.02E-73 | -1.69E-46 |
| MS.gene052414 | MS.gene71558 | 0.805832 | 1.10E-49 | -1.69E-46 |
| MS.gene052481 | MS.gene71558 | 0.848248 | 6.92E-60 | -1.69E-46 |
| MS.gene052484 | MS.gene71558 | 0.808299 | 3.32E-50 | -1.69E-46 |
| MS.gene052485 | MS.gene71558 | 0.853268 | 2.67E-61 | -1.69E-46 |
| MS.gene052505 | MS.gene71558 | 0.802859 | 4.59E-49 | -1.69E-46 |
| MS.gene052758 | MS.gene71558 | 0.812711 | 3.70E-51 | -1.69E-46 |
| MS.gene052982 | MS.gene71558 | 0.80681 | 6.86E-50 | -1.69E-46 |
| MS.gene053209 | MS.gene71558 | 0.849904 | 2.40E-60 | -1.69E-46 |
| MS.gene053330 | MS.gene71558 | 0.887531 | 1.31E-72 | -1.69E-46 |
| MS.gene053557 | MS.gene71558 | 0.847717 | 9.69E-60 | -1.69E-46 |
| MS.gene053836 | MS.gene71558 | 0.885223 | 9.79E-72 | -1.69E-46 |
| MS.gene053907 | MS.gene71558 | 0.833601 | 4.86E-56 | -1.69E-46 |
| MS.gene053928 | MS.gene71558 | 0.831041 | 2.09E-55 | -1.69E-46 |
| MS.gene053971 | MS.gene71558 | 0.844837 | 5.90E-59 | -1.69E-46 |
| MS.gene054102 | MS.gene71558 | 0.820699 | 5.98E-53 | -1.69E-46 |
| MS.gene054123 | MS.gene71558 | 0.803624 | 3.19E-49 | -1.69E-46 |
| MS.gene05413 | MS.gene71558 | 0.827132 | 1.85E-54 | -1.69E-46 |
| MS.gene054183 | MS.gene71558 | 0.868393 | 6.75E-66 | -1.69E-46 |
| MS.gene054349 | MS.gene71558 | 0.874563 | 6.12E-68 | -1.69E-46 |
| MS.gene054473 | MS.gene71558 | 0.841569 | 4.39E-58 | -1.69E-46 |
| MS.gene054556 | MS.gene71558 | 0.803873 | 2.83E-49 | -1.69E-46 |
| MS.gene054719 | MS.gene71558 | 0.846319 | 2.34E-59 | -1.69E-46 |
| MS.gene054918 | MS.gene71558 | 0.835211 | 1.92E-56 | -1.69E-46 |
| MS.gene054930 | MS.gene71558 | 0.826292 | 2.94E-54 | -1.69E-46 |
| MS.gene054983 | MS.gene71558 | 0.864438 | 1.22E-64 | -1.69E-46 |
| MS.gene055116 | MS.gene71558 | 0.915431 | 5.93E-85 | -1.69E-46 |
| MS.gene055277 | MS.gene71558 | 0.852658 | 4.00E-61 | -1.69E-46 |
| MS.gene055369 | MS.gene71558 | 0.844881 | 5.74E-59 | -1.69E-46 |
| MS.gene055370 | MS.gene71558 | 0.825866 | 3.71E-54 | -1.69E-46 |
| MS.gene055390 | MS.gene71558 | 0.80352 | 3.35E-49 | -1.69E-46 |
| MS.gene055422 | MS.gene71558 | 0.837662 | 4.55E-57 | -1.69E-46 |
| MS.gene055428 | MS.gene71558 | 0.843909 | 1.05E-58 | -1.69E-46 |
| MS.gene05553 | MS.gene71558 | 0.898929 | 3.27E-77 | -1.69E-46 |
| MS.gene055564 | MS.gene71558 | 0.843803 | 1.12E-58 | -1.69E-46 |
| MS.gene055601 | MS.gene71558 | -0.815204 | 1.04E-51 | -1.69E-46 |
| MS.gene055604 | MS.gene71558 | 0.828603 | 8.20E-55 | -1.69E-46 |
| MS.gene055859 | MS.gene71558 | 0.812908 | 3.35E-51 | -1.69E-46 |
| MS.gene05594 | MS.gene71558 | 0.826359 | 2.83E-54 | -1.69E-46 |
| MS.gene056191 | MS.gene71558 | 0.846123 | 2.65E-59 | -1.69E-46 |
| MS.gene056233 | MS.gene71558 | 0.805694 | 1.18E-49 | -1.69E-46 |
| MS.gene056337 | MS.gene71558 | 0.842682 | 2.23E-58 | -1.69E-46 |
| MS.gene056407 | MS.gene71558 | 0.851674 | 7.61E-61 | -1.69E-46 |
| MS.gene056451 | MS.gene71558 | 0.857452 | 1.62E-62 | -1.69E-46 |
| MS.gene056511 | MS.gene71558 | 0.819482 | 1.13E-52 | -1.69E-46 |
| MS.gene056612 | MS.gene71558 | 0.862014 | 6.84E-64 | -1.69E-46 |
| MS.gene056633 | MS.gene71558 | 0.82627 | 2.97E-54 | -1.69E-46 |
| MS.gene056659 | MS.gene71558 | 0.867178 | 1.66E-65 | -1.69E-46 |
| MS.gene056723 | MS.gene71558 | 0.871783 | 5.25E-67 | -1.69E-46 |
| MS.gene056796 | MS.gene71558 | 0.815845 | 7.50E-52 | -1.69E-46 |
| MS.gene056899 | MS.gene71558 | -0.862966 | 3.48E-64 | -1.69E-46 |
| MS.gene056979 | MS.gene71558 | 0.819125 | 1.37E-52 | -1.69E-46 |
| MS.gene05702 | MS.gene71558 | 0.892309 | 1.79E-74 | -1.69E-46 |
| MS.gene057121 | MS.gene71558 | 0.855231 | 7.25E-62 | -1.69E-46 |
| MS.gene057124 | MS.gene71558 | 0.856405 | 3.29E-62 | -1.69E-46 |
| MS.gene057245 | MS.gene71558 | 0.810652 | 1.04E-50 | -1.69E-46 |
| MS.gene05733 | MS.gene71558 | 0.806249 | 9.02E-50 | -1.69E-46 |
| MS.gene057396 | MS.gene71558 | 0.84686 | 1.67E-59 | -1.69E-46 |
| MS.gene057448 | MS.gene71558 | 0.842379 | 2.68E-58 | -1.69E-46 |
| MS.gene057449 | MS.gene71558 | 0.829511 | 4.94E-55 | -1.69E-46 |
| MS.gene057450 | MS.gene71558 | 0.83788 | 4.00E-57 | -1.69E-46 |
| MS.gene057476 | MS.gene71558 | 0.83676 | 7.75E-57 | -1.69E-46 |
| MS.gene057749 | MS.gene71558 | 0.863244 | 2.86E-64 | -1.69E-46 |
| MS.gene057823 | MS.gene71558 | 0.848144 | 7.39E-60 | -1.69E-46 |
| MS.gene057825 | MS.gene71558 | 0.835706 | 1.44E-56 | -1.69E-46 |
| MS.gene058002 | MS.gene71558 | 0.903817 | 2.34E-79 | -1.69E-46 |
| MS.gene058007 | MS.gene71558 | 0.827548 | 1.47E-54 | -1.69E-46 |
| MS.gene058082 | MS.gene71558 | 0.815873 | 7.40E-52 | -1.69E-46 |
| MS.gene05811 | MS.gene71558 | 0.866453 | 2.82E-65 | -1.69E-46 |
| MS.gene058142 | MS.gene71558 | 0.885562 | 7.31E-72 | -1.69E-46 |
| MS.gene058233 | MS.gene71558 | 0.890814 | 7.01E-74 | -1.69E-46 |
| MS.gene058276 | MS.gene71558 | 0.857327 | 1.76E-62 | -1.69E-46 |
| MS.gene058365 | MS.gene71558 | 0.840913 | 6.53E-58 | -1.69E-46 |
| MS.gene05842 | MS.gene71558 | 0.807389 | 5.18E-50 | -1.69E-46 |
| MS.gene058444 | MS.gene71558 | 0.80593 | 1.05E-49 | -1.69E-46 |
| MS.gene058660 | MS.gene71558 | 0.845157 | 4.84E-59 | -1.69E-46 |
| MS.gene058700 | MS.gene71558 | 0.816449 | 5.50E-52 | -1.69E-46 |
| MS.gene058710 | MS.gene71558 | 0.865769 | 4.64E-65 | -1.69E-46 |
| MS.gene058746 | MS.gene71558 | 0.858009 | 1.11E-62 | -1.69E-46 |
| MS.gene058749 | MS.gene71558 | 0.817547 | 3.12E-52 | -1.69E-46 |
| MS.gene058787 | MS.gene71558 | 0.801075 | 1.07E-48 | -1.69E-46 |
| MS.gene058845 | MS.gene71558 | 0.826734 | 2.31E-54 | -1.69E-46 |
| MS.gene05886 | MS.gene71558 | 0.86618 | 3.44E-65 | -1.69E-46 |
| MS.gene058928 | MS.gene71558 | 0.836333 | 9.95E-57 | -1.69E-46 |
| MS.gene058949 | MS.gene71558 | 0.803524 | 3.35E-49 | -1.69E-46 |
| MS.gene059011 | MS.gene71558 | 0.877034 | 8.68E-69 | -1.69E-46 |
| MS.gene05907 | MS.gene71558 | 0.88377 | 3.39E-71 | -1.69E-46 |
| MS.gene059082 | MS.gene71558 | 0.800075 | 1.71E-48 | -1.69E-46 |
| MS.gene059141 | MS.gene71558 | 0.84409 | 9.38E-59 | -1.69E-46 |
| MS.gene059247 | MS.gene71558 | 0.812657 | 3.80E-51 | -1.69E-46 |
| MS.gene059285 | MS.gene71558 | 0.854748 | 1.00E-61 | -1.69E-46 |
| MS.gene059294 | MS.gene71558 | 0.831199 | 1.91E-55 | -1.69E-46 |
| MS.gene059420 | MS.gene71558 | 0.893051 | 9.00E-75 | -1.69E-46 |
| MS.gene059462 | MS.gene71558 | 0.8283 | 9.71E-55 | -1.69E-46 |
| MS.gene059518 | MS.gene71558 | 0.831123 | 1.99E-55 | -1.69E-46 |
| MS.gene059597 | MS.gene71558 | 0.839448 | 1.57E-57 | -1.69E-46 |
| MS.gene059676 | MS.gene71558 | 0.831781 | 1.37E-55 | -1.69E-46 |
| MS.gene059688 | MS.gene71558 | 0.883299 | 5.05E-71 | -1.69E-46 |
| MS.gene059692 | MS.gene71558 | 0.889223 | 2.94E-73 | -1.69E-46 |
| MS.gene059695 | MS.gene71558 | 0.888089 | 8.04E-73 | -1.69E-46 |
| MS.gene05984 | MS.gene71558 | 0.868858 | 4.78E-66 | -1.69E-46 |
| MS.gene059894 | MS.gene71558 | 0.8669 | 2.03E-65 | -1.69E-46 |
| MS.gene060247 | MS.gene71558 | 0.828711 | 7.72E-55 | -1.69E-46 |
| MS.gene06037 | MS.gene71558 | 0.84603 | 2.80E-59 | -1.69E-46 |
| MS.gene060403 | MS.gene71558 | 0.858108 | 1.03E-62 | -1.69E-46 |
| MS.gene060407 | MS.gene71558 | 0.871821 | 5.10E-67 | -1.69E-46 |
| MS.gene060986 | MS.gene71558 | 0.809137 | 2.20E-50 | -1.69E-46 |
| MS.gene061049 | MS.gene71558 | 0.912868 | 1.19E-83 | -1.69E-46 |
| MS.gene061051 | MS.gene71558 | -0.816268 | 6.04E-52 | -1.69E-46 |
| MS.gene061114 | MS.gene71558 | -0.826104 | 3.26E-54 | -1.69E-46 |
| MS.gene061154 | MS.gene71558 | 0.860679 | 1.75E-63 | -1.69E-46 |
| MS.gene061159 | MS.gene71558 | 0.868337 | 7.04E-66 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71558.t1 | MTR_7g032900 | 95.397 | 239 | 11 | 0 | 32 | 270 | 46 | 284 | 2.25e-171 | 474 |
| MS.gene71558.t1 | MTR_6g092630 | 40.722 | 194 | 111 | 1 | 39 | 228 | 95 | 288 | 6.30e-46 | 156 |
| MS.gene71558.t1 | MTR_6g092630 | 41.026 | 195 | 111 | 1 | 39 | 229 | 95 | 289 | 6.92e-46 | 156 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71558.t1 | AT3G01660 | 67.364 | 239 | 75 | 1 | 31 | 269 | 38 | 273 | 1.13e-121 | 348 |
| MS.gene71558.t1 | AT4G29590 | 40.415 | 193 | 111 | 1 | 41 | 229 | 106 | 298 | 1.74e-44 | 153 |
Find 57 sgRNAs with CRISPR-Local
Find 99 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTAGCTGAAATGAAATTAA+AGG | 0.190982 | 7.3:-66789796 | None:intergenic |
| TTGTATACCATATGCATTAT+TGG | 0.206333 | 7.3:-66789868 | None:intergenic |
| CCCTTCTCTCCATGCACTTA+TGG | 0.211437 | 7.3:-66791095 | None:intergenic |
| TCTATGCAGTTATTGCTTAT+AGG | 0.219291 | 7.3:+66791281 | MS.gene71558:CDS |
| TGTATACCATATGCATTATT+GGG | 0.235224 | 7.3:-66789867 | None:intergenic |
| ATCTTGGATAAGGTTCAATT+TGG | 0.247707 | 7.3:-66790125 | None:intergenic |
| TTCTTGATCTCATGAGTTCT+TGG | 0.266453 | 7.3:+66790251 | MS.gene71558:CDS |
| AGCACGGTCATGAAGCTCTT+TGG | 0.299546 | 7.3:+66791235 | MS.gene71558:CDS |
| ATGAAATTAAAGGCATCTTT+TGG | 0.317779 | 7.3:-66789786 | None:intergenic |
| AGGTATTTGCGGAGGTGTTT+AGG | 0.319706 | 7.3:+66791011 | MS.gene71558:intron |
| GTTGGATTACTTTGTCATAA+AGG | 0.320022 | 7.3:+66790357 | MS.gene71558:CDS |
| ATGCATAAAACTCTTTATCT+TGG | 0.343449 | 7.3:-66790141 | None:intergenic |
| AAAACTCTTTATCTTGGATA+AGG | 0.357532 | 7.3:-66790135 | None:intergenic |
| GAGAAGGGACTACTTATAGT+AGG | 0.371810 | 7.3:+66791110 | MS.gene71558:CDS |
| CTGTGAAACCTTCCACTGAT+TGG | 0.374068 | 7.3:-66791156 | None:intergenic |
| CCAATTTAATCATCATGTAT+TGG | 0.377264 | 7.3:-66791313 | None:intergenic |
| ATCTAACCTTCTCTGGTTGT+TGG | 0.398077 | 7.3:-66790459 | None:intergenic |
| TAACAAGTCTTTACAGAGAA+AGG | 0.413091 | 7.3:+66790209 | MS.gene71558:CDS |
| GGAATATATCTAACCTTCTC+TGG | 0.413293 | 7.3:-66790466 | None:intergenic |
| TCTTGATCTCATGAGTTCTT+GGG | 0.417962 | 7.3:+66790252 | MS.gene71558:CDS |
| AGATCAAGAATCTCTATGTT+AGG | 0.439399 | 7.3:-66790238 | None:intergenic |
| GTAAAATACAAAAGAGTGGT+AGG | 0.461440 | 7.3:+66790295 | MS.gene71558:CDS |
| GTGGTAGGGCATGGGATGAA+TGG | 0.478315 | 7.3:+66790310 | MS.gene71558:CDS |
| CTTACTATGAACACCCCTCC+TGG | 0.496919 | 7.3:-66791043 | None:intergenic |
| AGATAATTCACAGGTATTTG+CGG | 0.498595 | 7.3:+66791000 | MS.gene71558:intron |
| TACAAAAGAGTGGTAGGGCA+TGG | 0.506435 | 7.3:+66790301 | MS.gene71558:CDS |
| TAAGCAATAACTGCATAGAA+AGG | 0.509040 | 7.3:-66791277 | None:intergenic |
| GCCATAAGTGCATGGAGAGA+AGG | 0.518478 | 7.3:+66791094 | MS.gene71558:CDS |
| GGAAGGTTTCACAGAGGCAG+AGG | 0.525489 | 7.3:+66791165 | MS.gene71558:CDS |
| ACTTGCAAAGAATCCAAGGT+TGG | 0.534361 | 7.3:+66790339 | MS.gene71558:CDS |
| ATGACAAAGTAATCCAACCT+TGG | 0.540131 | 7.3:-66790352 | None:intergenic |
| TAGTGGTGACTTGTCATCAA+TGG | 0.540340 | 7.3:-66791209 | None:intergenic |
| CCATAAGTGCATGGAGAGAA+GGG | 0.541068 | 7.3:+66791095 | MS.gene71558:CDS |
| TTATAGTAGGGTGCGACTTG+TGG | 0.542539 | 7.3:+66791123 | MS.gene71558:CDS |
| GTATACCATATGCATTATTG+GGG | 0.543687 | 7.3:-66789866 | None:intergenic |
| GACTTGTCATCAATGGTAGT+TGG | 0.545370 | 7.3:-66791202 | None:intergenic |
| CAGTACTTCCAATCAGTGGA+AGG | 0.551004 | 7.3:+66791148 | MS.gene71558:CDS |
| CTTGTGACTCATGTTGATGA+TGG | 0.555804 | 7.3:+66790172 | MS.gene71558:CDS |
| ACAAAAGAGTGGTAGGGCAT+GGG | 0.567245 | 7.3:+66790302 | MS.gene71558:CDS |
| GTGTTTAGGTTGCTAAAACC+AGG | 0.582281 | 7.3:+66791025 | MS.gene71558:CDS |
| TTCATGACCGTGCTCAGTAG+TGG | 0.584220 | 7.3:-66791226 | None:intergenic |
| TAAAATACAAAAGAGTGGTA+GGG | 0.591010 | 7.3:+66790296 | MS.gene71558:CDS |
| AAGAACTTGCAAAGAATCCA+AGG | 0.593998 | 7.3:+66790335 | MS.gene71558:CDS |
| AGAAGGGACTACTTATAGTA+GGG | 0.601293 | 7.3:+66791111 | MS.gene71558:CDS |
| ATGAGAAAGCCATAAGTGCA+TGG | 0.601564 | 7.3:+66791086 | MS.gene71558:CDS |
| TCAACATGAGTCACAAGGCG+TGG | 0.605771 | 7.3:-66790166 | None:intergenic |
| GGTTCAGTACTTCCAATCAG+TGG | 0.616659 | 7.3:+66791144 | MS.gene71558:CDS |
| GTATCTCCAACAACCAGAGA+AGG | 0.623557 | 7.3:+66790453 | MS.gene71558:CDS |
| TAATTCACAGGTATTTGCGG+AGG | 0.627593 | 7.3:+66791003 | MS.gene71558:intron |
| TAGGTTGCTAAAACCAGGAG+GGG | 0.635714 | 7.3:+66791030 | MS.gene71558:CDS |
| CATCATCAACATGAGTCACA+AGG | 0.647019 | 7.3:-66790171 | None:intergenic |
| TGATGTAAAATACAAAAGAG+TGG | 0.649830 | 7.3:+66790291 | MS.gene71558:CDS |
| CAAGTCACCACTACTGAGCA+CGG | 0.656964 | 7.3:+66791219 | MS.gene71558:CDS |
| TATACCATATGCATTATTGG+GGG | 0.671620 | 7.3:-66789865 | None:intergenic |
| TTTAGGTTGCTAAAACCAGG+AGG | 0.688060 | 7.3:+66791028 | MS.gene71558:CDS |
| TTAGGTTGCTAAAACCAGGA+GGG | 0.690482 | 7.3:+66791029 | MS.gene71558:CDS |
| ATCAGTGGAAGGTTTCACAG+AGG | 0.782577 | 7.3:+66791159 | MS.gene71558:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCAAAATTATAAAAATATCC+CGG | + | chr7.3:66790652-66790671 | MS.gene71558:intron | 15.0% |
| !!! | ATTTAATGTGTTTACTATTT+TGG | - | chr7.3:66790029-66790048 | None:intergenic | 15.0% |
| !!! | TTATAATTTTGATACATTCA+AGG | - | chr7.3:66790644-66790663 | None:intergenic | 15.0% |
| !!! | CTTTTGTATTTTACATCATT+TGG | - | chr7.3:66790289-66790308 | None:intergenic | 20.0% |
| ! | AAAACTCTTTATCTTGGATA+AGG | - | chr7.3:66790138-66790157 | None:intergenic | 25.0% |
| ! | AAGGTTTGGAAAATTATATG+AGG | - | chr7.3:66790496-66790515 | None:intergenic | 25.0% |
| ! | AATTTCAAACAGTTAATCGA+AGG | + | chr7.3:66790570-66790589 | MS.gene71558:intron | 25.0% |
| ! | ATAGTCGAACATAAATTCTT+GGG | - | chr7.3:66790943-66790962 | None:intergenic | 25.0% |
| ! | ATGCATAAAACTCTTTATCT+TGG | - | chr7.3:66790144-66790163 | None:intergenic | 25.0% |
| ! | CACTAATAAAGATAATTCAC+AGG | + | chr7.3:66790991-66791010 | MS.gene71558:intron | 25.0% |
| ! | TAAAATACAAAAGAGTGGTA+GGG | + | chr7.3:66790296-66790315 | MS.gene71558:CDS | 25.0% |
| ! | TATAGTCGAACATAAATTCT+TGG | - | chr7.3:66790944-66790963 | None:intergenic | 25.0% |
| ! | TGATGTAAAATACAAAAGAG+TGG | + | chr7.3:66790291-66790310 | MS.gene71558:CDS | 25.0% |
| ! | TGTAGCTGAAATGAAATTAA+AGG | - | chr7.3:66789799-66789818 | None:intergenic | 25.0% |
| ! | TGTATACCATATGCATTATT+GGG | - | chr7.3:66789870-66789889 | None:intergenic | 25.0% |
| ! | TTGTATACCATATGCATTAT+TGG | - | chr7.3:66789871-66789890 | None:intergenic | 25.0% |
| !! | TTGAAATTTCTCAGTAGTTT+TGG | - | chr7.3:66790558-66790577 | None:intergenic | 25.0% |
| !! | TTTGAGATTGACAAAAGATA+AGG | - | chr7.3:66790515-66790534 | None:intergenic | 25.0% |
| !!! | AATGTGTTTACTATTTTGGT+TGG | - | chr7.3:66790025-66790044 | None:intergenic | 25.0% |
| !!! | ATGAAATTAAAGGCATCTTT+TGG | - | chr7.3:66789789-66789808 | None:intergenic | 25.0% |
| AATCAACAATCTTCTTCACT+AGG | + | chr7.3:66790064-66790083 | MS.gene71558:intron | 30.0% | |
| AGATAATTCACAGGTATTTG+CGG | + | chr7.3:66791000-66791019 | MS.gene71558:intron | 30.0% | |
| AGATCAAGAATCTCTATGTT+AGG | - | chr7.3:66790241-66790260 | None:intergenic | 30.0% | |
| ATCTTGGATAAGGTTCAATT+TGG | - | chr7.3:66790128-66790147 | None:intergenic | 30.0% | |
| GATTGACAAAAGATAAGGTT+TGG | - | chr7.3:66790510-66790529 | None:intergenic | 30.0% | |
| GTAAAATACAAAAGAGTGGT+AGG | + | chr7.3:66790295-66790314 | MS.gene71558:CDS | 30.0% | |
| GTATACCATATGCATTATTG+GGG | - | chr7.3:66789869-66789888 | None:intergenic | 30.0% | |
| GTTGGATTACTTTGTCATAA+AGG | + | chr7.3:66790357-66790376 | MS.gene71558:CDS | 30.0% | |
| TAACAAGTCTTTACAGAGAA+AGG | + | chr7.3:66790209-66790228 | MS.gene71558:CDS | 30.0% | |
| TAAGCAATAACTGCATAGAA+AGG | - | chr7.3:66791280-66791299 | None:intergenic | 30.0% | |
| TAATCGAAGGAGTAAATAGT+CGG | + | chr7.3:66790583-66790602 | MS.gene71558:intron | 30.0% | |
| TAGTGAAGAAGATTGTTGAT+TGG | - | chr7.3:66790065-66790084 | None:intergenic | 30.0% | |
| TATACCATATGCATTATTGG+GGG | - | chr7.3:66789868-66789887 | None:intergenic | 30.0% | |
| TCTATGCAGTTATTGCTTAT+AGG | + | chr7.3:66791281-66791300 | MS.gene71558:CDS | 30.0% | |
| TGGAAAATTATATGAGGTCA+TGG | - | chr7.3:66790490-66790509 | None:intergenic | 30.0% | |
| ! | AAGAAGATTGTTGATTGGTT+TGG | - | chr7.3:66790060-66790079 | None:intergenic | 30.0% |
| ! | CAATCTCAAATCTCAAGTTT+TGG | + | chr7.3:66790525-66790544 | MS.gene71558:intron | 30.0% |
| ! | GTCTTTTGCTAGTAATATCA+TGG | - | chr7.3:66790749-66790768 | None:intergenic | 30.0% |
| !!! | ATTAAAGGCATCTTTTGGTT+CGG | - | chr7.3:66789784-66789803 | None:intergenic | 30.0% |
| AAGAACTTGCAAAGAATCCA+AGG | + | chr7.3:66790335-66790354 | MS.gene71558:CDS | 35.0% | |
| AGAAGGGACTACTTATAGTA+GGG | + | chr7.3:66791111-66791130 | MS.gene71558:CDS | 35.0% | |
| ATGAAATGATTGTGATGTCG+CGG | + | chr7.3:66790911-66790930 | MS.gene71558:intron | 35.0% | |
| ATGACAAAGTAATCCAACCT+TGG | - | chr7.3:66790355-66790374 | None:intergenic | 35.0% | |
| ATTGGGGGATAAGTAATCAA+TGG | - | chr7.3:66789853-66789872 | None:intergenic | 35.0% | |
| GGAATATATCTAACCTTCTC+TGG | - | chr7.3:66790469-66790488 | None:intergenic | 35.0% | |
| TCTTGATCTCATGAGTTCTT+GGG | + | chr7.3:66790252-66790271 | MS.gene71558:CDS | 35.0% | |
| TTATCCCCCAATAATGCATA+TGG | + | chr7.3:66789861-66789880 | MS.gene71558:CDS | 35.0% | |
| TTCTTGATCTCATGAGTTCT+TGG | + | chr7.3:66790251-66790270 | MS.gene71558:CDS | 35.0% | |
| TTTGATATGAGGTGTGTACA+TGG | - | chr7.3:66789999-66790018 | None:intergenic | 35.0% | |
| ! | GTGTTTTTCGTTAGTCAGTT+TGG | + | chr7.3:66790691-66790710 | MS.gene71558:intron | 35.0% |
| !! | CGTTTTGTTCTTACACAAGA+AGG | + | chr7.3:66790097-66790116 | MS.gene71558:CDS | 35.0% |
| !! | CTCAAATCTCAAGTTTTGGT+AGG | + | chr7.3:66790529-66790548 | MS.gene71558:intron | 35.0% |
| !! | TGTAGATTGGTTTGCATTGT+TGG | - | chr7.3:66789974-66789993 | None:intergenic | 35.0% |
| !! | TTGGTTGGAGTTTTGATATG+AGG | - | chr7.3:66790010-66790029 | None:intergenic | 35.0% |
| AAGGAGTAAATAGTCGGTCT+TGG | + | chr7.3:66790589-66790608 | MS.gene71558:intron | 40.0% | |
| ACTTGCAAAGAATCCAAGGT+TGG | + | chr7.3:66790339-66790358 | MS.gene71558:CDS | 40.0% | |
| ATCTAACCTTCTCTGGTTGT+TGG | - | chr7.3:66790462-66790481 | None:intergenic | 40.0% | |
| ATGAGAAAGCCATAAGTGCA+TGG | + | chr7.3:66791086-66791105 | MS.gene71558:CDS | 40.0% | |
| CATCATCAACATGAGTCACA+AGG | - | chr7.3:66790174-66790193 | None:intergenic | 40.0% | |
| CTGATCAAATGTGTCTCATG+TGG | + | chr7.3:66790858-66790877 | MS.gene71558:intron | 40.0% | |
| GACTAACAGAGACATGTTTG+TGG | - | chr7.3:66790718-66790737 | None:intergenic | 40.0% | |
| GACTTGTCATCAATGGTAGT+TGG | - | chr7.3:66791205-66791224 | None:intergenic | 40.0% | |
| GAGAAGGGACTACTTATAGT+AGG | + | chr7.3:66791110-66791129 | MS.gene71558:CDS | 40.0% | |
| GTAGCTATTTGGGTGAAAAG+TGG | - | chr7.3:66789943-66789962 | None:intergenic | 40.0% | |
| TAATTCACAGGTATTTGCGG+AGG | + | chr7.3:66791003-66791022 | MS.gene71558:intron | 40.0% | |
| TATTTGCGACAACACAACTG+TGG | - | chr7.3:66790890-66790909 | None:intergenic | 40.0% | |
| TGTGTACATGGCATGTAGAT+TGG | - | chr7.3:66789987-66790006 | None:intergenic | 40.0% | |
| ! | CTTGTGACTCATGTTGATGA+TGG | + | chr7.3:66790172-66790191 | MS.gene71558:CDS | 40.0% |
| ! | GGAGTTTGGAGTAGCTATTT+GGG | - | chr7.3:66789953-66789972 | None:intergenic | 40.0% |
| ! | TAGTGGTGACTTGTCATCAA+TGG | - | chr7.3:66791212-66791231 | None:intergenic | 40.0% |
| ! | TATCCCGGAGTCTTTCTTTT+TGG | + | chr7.3:66790667-66790686 | MS.gene71558:intron | 40.0% |
| ! | TGGAGTTTGGAGTAGCTATT+TGG | - | chr7.3:66789954-66789973 | None:intergenic | 40.0% |
| ! | TGGTTTGCATTGTTGGAGTT+TGG | - | chr7.3:66789967-66789986 | None:intergenic | 40.0% |
| ! | TTAGGTTGCTAAAACCAGGA+GGG | + | chr7.3:66791029-66791048 | MS.gene71558:CDS | 40.0% |
| !! | GTGTTTAGGTTGCTAAAACC+AGG | + | chr7.3:66791025-66791044 | MS.gene71558:CDS | 40.0% |
| !! | TTTAGGTTGCTAAAACCAGG+AGG | + | chr7.3:66791028-66791047 | MS.gene71558:CDS | 40.0% |
| ACAAAAGAGTGGTAGGGCAT+GGG | + | chr7.3:66790302-66790321 | MS.gene71558:CDS | 45.0% | |
| ACGCCAAAAAGAAAGACTCC+GGG | - | chr7.3:66790673-66790692 | None:intergenic | 45.0% | |
| ATCAGTGGAAGGTTTCACAG+AGG | + | chr7.3:66791159-66791178 | MS.gene71558:CDS | 45.0% | |
| CACGCCAAAAAGAAAGACTC+CGG | - | chr7.3:66790674-66790693 | None:intergenic | 45.0% | |
| CAGTACTTCCAATCAGTGGA+AGG | + | chr7.3:66791148-66791167 | MS.gene71558:CDS | 45.0% | |
| CCATAAGTGCATGGAGAGAA+GGG | + | chr7.3:66791095-66791114 | MS.gene71558:CDS | 45.0% | |
| CTGTGAAACCTTCCACTGAT+TGG | - | chr7.3:66791159-66791178 | None:intergenic | 45.0% | |
| GGTTCAGTACTTCCAATCAG+TGG | + | chr7.3:66791144-66791163 | MS.gene71558:CDS | 45.0% | |
| GTATCTCCAACAACCAGAGA+AGG | + | chr7.3:66790453-66790472 | MS.gene71558:CDS | 45.0% | |
| TACAAAAGAGTGGTAGGGCA+TGG | + | chr7.3:66790301-66790320 | MS.gene71558:CDS | 45.0% | |
| TAGGTTGCTAAAACCAGGAG+GGG | + | chr7.3:66791030-66791049 | MS.gene71558:CDS | 45.0% | |
| TTATAGTAGGGTGCGACTTG+TGG | + | chr7.3:66791123-66791142 | MS.gene71558:CDS | 45.0% | |
| !! | AGGTATTTGCGGAGGTGTTT+AGG | + | chr7.3:66791011-66791030 | MS.gene71558:intron | 45.0% |
| AGCACGGTCATGAAGCTCTT+TGG | + | chr7.3:66791235-66791254 | MS.gene71558:CDS | 50.0% | |
| CAAGTCACCACTACTGAGCA+CGG | + | chr7.3:66791219-66791238 | MS.gene71558:CDS | 50.0% | |
| CCCTTCTCTCCATGCACTTA+TGG | - | chr7.3:66791098-66791117 | None:intergenic | 50.0% | |
| CGACAACACAACTGTGGTTG+AGG | - | chr7.3:66790884-66790903 | None:intergenic | 50.0% | |
| CTTACTATGAACACCCCTCC+TGG | - | chr7.3:66791046-66791065 | None:intergenic | 50.0% | |
| GCCATAAGTGCATGGAGAGA+AGG | + | chr7.3:66791094-66791113 | MS.gene71558:CDS | 50.0% | |
| TCAACATGAGTCACAAGGCG+TGG | - | chr7.3:66790169-66790188 | None:intergenic | 50.0% | |
| TTCATGACCGTGCTCAGTAG+TGG | - | chr7.3:66791229-66791248 | None:intergenic | 50.0% | |
| GGAAGGTTTCACAGAGGCAG+AGG | + | chr7.3:66791165-66791184 | MS.gene71558:CDS | 55.0% | |
| GTGGTAGGGCATGGGATGAA+TGG | + | chr7.3:66790310-66790329 | MS.gene71558:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 66789777 | 66791330 | 66789777 | ID=MS.gene71558 |
| chr7.3 | mRNA | 66789777 | 66791330 | 66789777 | ID=MS.gene71558.t1;Parent=MS.gene71558 |
| chr7.3 | exon | 66789777 | 66789882 | 66789777 | ID=MS.gene71558.t1.exon1;Parent=MS.gene71558.t1 |
| chr7.3 | CDS | 66789777 | 66789882 | 66789777 | ID=cds.MS.gene71558.t1;Parent=MS.gene71558.t1 |
| chr7.3 | exon | 66790086 | 66790474 | 66790086 | ID=MS.gene71558.t1.exon2;Parent=MS.gene71558.t1 |
| chr7.3 | CDS | 66790086 | 66790474 | 66790086 | ID=cds.MS.gene71558.t1;Parent=MS.gene71558.t1 |
| chr7.3 | exon | 66791013 | 66791330 | 66791013 | ID=MS.gene71558.t1.exon3;Parent=MS.gene71558.t1 |
| chr7.3 | CDS | 66791013 | 66791330 | 66791013 | ID=cds.MS.gene71558.t1;Parent=MS.gene71558.t1 |
| Gene Sequence |
| Protein sequence |
