AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene62645


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62645.t1 MTR_8g106210 95.494 466 5 3 3 468 24 473 0.0 911
MS.gene62645.t1 MTR_4g066010 34.198 424 238 9 57 450 172 584 8.74e-74 243
MS.gene62645.t1 MTR_2g098420 34.925 398 230 6 70 443 188 580 5.23e-73 242
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62645.t1 AT1G12330 55.580 457 147 11 23 449 42 472 6.15e-172 493
MS.gene62645.t1 AT5G12900 41.139 316 179 4 138 450 247 558 1.32e-80 260

Find 63 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCAAACATGGTTAGGATTA+TGG 0.210083 4.2:+901113 MS.gene62645:CDS
TTCAGTTTACATGGAAGATA+TGG 0.260469 4.2:+901065 MS.gene62645:CDS
CAACGTTGTACAACATTTCT+TGG 0.272548 4.2:+900504 MS.gene62645:CDS
TTTGTTGTTGCTGAGGTTAT+TGG 0.282756 4.2:-901199 None:intergenic
TATTGTCACACTTTGAAATC+TGG 0.312009 4.2:+900382 MS.gene62645:CDS
TTCAAAAGAATGCTTGTAAT+AGG 0.330527 4.2:+900743 MS.gene62645:CDS
TGCTATGTTGGGTTGGAATA+GGG 0.335899 4.2:+900915 MS.gene62645:CDS
TTGCTATGTTGGGTTGGAAT+AGG 0.340560 4.2:+900914 MS.gene62645:CDS
GCTTCATTCAACATGATTCA+TGG 0.354899 4.2:+900802 MS.gene62645:CDS
TTAAATGTAAGGTTCTTTGC+AGG 0.355993 4.2:+901169 MS.gene62645:CDS
GCTGTTACTGCTGTTGTTGT+TGG 0.357262 4.2:-900113 None:intergenic
CAATGAAGAACAATGGAAAA+TGG 0.357639 4.2:+900234 MS.gene62645:CDS
AATACTGCTACTAATGTTGT+TGG 0.374603 4.2:+900427 MS.gene62645:CDS
ATGGATTTGAAAATCCCATT+TGG 0.381416 4.2:-900160 None:intergenic
TTGTTGCTTCAAAGAGTGTT+TGG 0.389896 4.2:+900968 MS.gene62645:CDS
TTGGTGTTGTTCTTCTACTT+TGG 0.395204 4.2:-899901 None:intergenic
TGTTGGGTTGGAATAGGGCT+TGG 0.406333 4.2:+900920 MS.gene62645:CDS
AAGCTCTTCAAGAAGCTTCA+AGG 0.420955 4.2:+900308 MS.gene62645:CDS
CAACTTTGATTCAGTTTACA+TGG 0.421670 4.2:+901056 MS.gene62645:CDS
GGTTGGTACCAAACATGGTT+AGG 0.425331 4.2:+901106 MS.gene62645:CDS
ACCATAATCCTAACCATGTT+TGG 0.426702 4.2:-901114 None:intergenic
ACTTGATGGGTTTAAGGTGA+TGG 0.428119 4.2:-901240 None:intergenic
ACATGATTCATGGGTTGACT+TGG 0.437332 4.2:+900812 MS.gene62645:CDS
TTTGGAGTTGAAGCACTTGT+TGG 0.439726 4.2:-899920 None:intergenic
CTTTGTTGCAAAGTCCAAAT+GGG 0.440057 4.2:+900146 MS.gene62645:CDS
TGTTTGGATGGTTCATTTGT+TGG 0.441093 4.2:+900984 MS.gene62645:CDS
TAATAGGATATTGAACCCTA+TGG 0.449841 4.2:+900759 MS.gene62645:CDS
GTTAGGATTATGGTTGCTCC+TGG 0.452810 4.2:+901123 MS.gene62645:CDS
TCAGTTTACATGGAAGATAT+GGG 0.453427 4.2:+901066 MS.gene62645:CDS
CTTCATTCAACATGATTCAT+GGG 0.453738 4.2:+900803 MS.gene62645:CDS
CTTGATGGGTTTAAGGTGAT+GGG 0.456935 4.2:-901239 None:intergenic
TTGATATTGATTGGTGTTGG+TGG 0.458109 4.2:-899965 None:intergenic
TCTTTGTTGCAAAGTCCAAA+TGG 0.458726 4.2:+900145 MS.gene62645:CDS
TGATGATTGATATTGTGCTT+TGG 0.465945 4.2:-899938 None:intergenic
AGTGAAATTGTTGCTATGTT+GGG 0.472103 4.2:+900904 MS.gene62645:CDS
TTGTTGATATTGATTGGTGT+TGG 0.475429 4.2:-899968 None:intergenic
GAGTGAAATTGTTGCTATGT+TGG 0.475944 4.2:+900903 MS.gene62645:CDS
CTTGATTCACACCTTTCCAT+AGG 0.488970 4.2:-900775 None:intergenic
TGGTAGTGCTGTTAAATGTA+AGG 0.493435 4.2:+901158 MS.gene62645:CDS
TTTGTTGTTGATGCTGACAT+TGG 0.496621 4.2:-900208 None:intergenic
ACAAAGAAAGCTTGCAGAAG+TGG 0.497139 4.2:-900949 None:intergenic
TCTTGTTAGCTTTGAAACAA+TGG 0.500929 4.2:-900179 None:intergenic
CGTTGTACAACATTTCTTGG+TGG 0.514695 4.2:+900507 MS.gene62645:CDS
TTGATTCACACCTTTCCATA+GGG 0.520693 4.2:-900774 None:intergenic
TTCAAGGTTGGTACCAAACA+TGG 0.524833 4.2:+901101 MS.gene62645:CDS
TGTCTTGTTTGTTGTTGCTG+AGG 0.529272 4.2:-901206 None:intergenic
TGGGTGGGGACAAGTCTTCA+AGG 0.532792 4.2:+901085 MS.gene62645:CDS
AAATTGTTGCTATGTTGGGT+TGG 0.545805 4.2:+900908 MS.gene62645:CDS
TGCTTCAAAGAGTGTTTGGA+TGG 0.549445 4.2:+900972 MS.gene62645:CDS
ATTAAATATGCAGATTCAAG+AGG 0.551509 4.2:+899838 None:intergenic
TCTGAAACTGAACTAGAAGA+TGG 0.555922 4.2:+900022 MS.gene62645:CDS
GTTGTTGTTGGAATGATCAA+AGG 0.567800 4.2:-900101 None:intergenic
CATGATTCATGGGTTGACTT+GGG 0.593679 4.2:+900813 MS.gene62645:CDS
GGATATTGAACCCTATGGAA+AGG 0.598272 4.2:+900764 MS.gene62645:CDS
TTTACATGGAAGATATGGGT+GGG 0.604216 4.2:+901070 MS.gene62645:CDS
TTGTTGTTGGAATGATCAAA+GGG 0.606933 4.2:-900100 None:intergenic
CAAACAACAATGAAGAACAA+TGG 0.609170 4.2:+900227 MS.gene62645:CDS
TTACATGGAAGATATGGGTG+GGG 0.625227 4.2:+901071 MS.gene62645:CDS
TGGGGACAAGTCTTCAAGGT+TGG 0.629308 4.2:+901089 MS.gene62645:CDS
GTTTACATGGAAGATATGGG+TGG 0.633305 4.2:+901069 MS.gene62645:CDS
ACAATGCAACTTAGACACAC+AGG 0.633999 4.2:+900577 MS.gene62645:CDS
TGTTGGAATGATCAAAGGGA+AGG 0.654140 4.2:-900096 None:intergenic
AGCAACAACAAACAAGACAA+AGG 0.670945 4.2:+901210 MS.gene62645:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAGAGATTTTTATATCATA+AGG - chr4.2:900640-900659 None:intergenic 15.0%
! AAGTAAAAAAGCAAACTTCT+TGG - chr4.2:900673-900692 None:intergenic 25.0%
! TTCAAAAGAATGCTTGTAAT+AGG + chr4.2:900743-900762 MS.gene62645:CDS 25.0%
!! CCTTTATCAACTCTAAAAAT+TGG - chr4.2:901033-901052 None:intergenic 25.0%
!!! AAGAAGTTTGCTTTTTTACT+TGG + chr4.2:900672-900691 MS.gene62645:CDS 25.0%
!!! CCAATTTTTAGAGTTGATAA+AGG + chr4.2:901030-901049 MS.gene62645:CDS 25.0%
!!! GTGTTTTTGTTGATATTGAT+TGG - chr4.2:899977-899996 None:intergenic 25.0%
!!! TTTGAACAAGACATTTTTTG+AGG + chr4.2:900702-900721 MS.gene62645:CDS 25.0%
AATACTGCTACTAATGTTGT+TGG + chr4.2:900427-900446 MS.gene62645:CDS 30.0%
AGTGAAATTGTTGCTATGTT+GGG + chr4.2:900904-900923 MS.gene62645:CDS 30.0%
ATGGATTTGAAAATCCCATT+TGG - chr4.2:900163-900182 None:intergenic 30.0%
CAAACAACAATGAAGAACAA+TGG + chr4.2:900227-900246 MS.gene62645:CDS 30.0%
CAACTTTGATTCAGTTTACA+TGG + chr4.2:901056-901075 MS.gene62645:CDS 30.0%
CAATGAAGAACAATGGAAAA+TGG + chr4.2:900234-900253 MS.gene62645:CDS 30.0%
CTTCATTCAACATGATTCAT+GGG + chr4.2:900803-900822 MS.gene62645:CDS 30.0%
TAATAGGATATTGAACCCTA+TGG + chr4.2:900759-900778 MS.gene62645:CDS 30.0%
TCAGTTTACATGGAAGATAT+GGG + chr4.2:901066-901085 MS.gene62645:CDS 30.0%
TGATGATTGATATTGTGCTT+TGG - chr4.2:899941-899960 None:intergenic 30.0%
TTAAATGTAAGGTTCTTTGC+AGG + chr4.2:901169-901188 MS.gene62645:CDS 30.0%
TTCAGTTTACATGGAAGATA+TGG + chr4.2:901065-901084 MS.gene62645:CDS 30.0%
! ACTCTAAAAATTGGCAAACT+TGG - chr4.2:901024-901043 None:intergenic 30.0%
! TTGTTGATATTGATTGGTGT+TGG - chr4.2:899971-899990 None:intergenic 30.0%
! TTGTTGTTGGAATGATCAAA+GGG - chr4.2:900103-900122 None:intergenic 30.0%
!! TATTGTCACACTTTGAAATC+TGG + chr4.2:900382-900401 MS.gene62645:CDS 30.0%
!! TCTTGTTAGCTTTGAAACAA+TGG - chr4.2:900182-900201 None:intergenic 30.0%
!! TTGAGGATTTTGAGTCAATT+GGG + chr4.2:900719-900738 MS.gene62645:CDS 30.0%
!! TTTGAGGATTTTGAGTCAAT+TGG + chr4.2:900718-900737 MS.gene62645:CDS 30.0%
!!! GAACGTTTTTTCTTGTGATT+TGG - chr4.2:900004-900023 None:intergenic 30.0%
AAATTGTTGCTATGTTGGGT+TGG + chr4.2:900908-900927 MS.gene62645:CDS 35.0%
ACCAAACATGGTTAGGATTA+TGG + chr4.2:901113-901132 MS.gene62645:CDS 35.0%
ACCATAATCCTAACCATGTT+TGG - chr4.2:901117-901136 None:intergenic 35.0%
AGCAACAACAAACAAGACAA+AGG + chr4.2:901210-901229 MS.gene62645:CDS 35.0%
CAACGTTGTACAACATTTCT+TGG + chr4.2:900504-900523 MS.gene62645:CDS 35.0%
CTACCATAGACATAAAAACC+AGG - chr4.2:901144-901163 None:intergenic 35.0%
CTTTGTTGCAAAGTCCAAAT+GGG + chr4.2:900146-900165 MS.gene62645:CDS 35.0%
GAGTGAAATTGTTGCTATGT+TGG + chr4.2:900903-900922 MS.gene62645:CDS 35.0%
GCTTCATTCAACATGATTCA+TGG + chr4.2:900802-900821 MS.gene62645:CDS 35.0%
TCTTTGTTGCAAAGTCCAAA+TGG + chr4.2:900145-900164 MS.gene62645:CDS 35.0%
TGTTTGGATGGTTCATTTGT+TGG + chr4.2:900984-901003 MS.gene62645:CDS 35.0%
TTGATTCACACCTTTCCATA+GGG - chr4.2:900777-900796 None:intergenic 35.0%
TTTACATGGAAGATATGGGT+GGG + chr4.2:901070-901089 MS.gene62645:CDS 35.0%
! AAGATTTTGTCACGAGAATG+TGG - chr4.2:900073-900092 None:intergenic 35.0%
! AGCTTCATCTCTTTTTCTTG+TGG - chr4.2:900293-900312 None:intergenic 35.0%
! GTTGTTGTTGGAATGATCAA+AGG - chr4.2:900104-900123 None:intergenic 35.0%
! TCTGAAACTGAACTAGAAGA+TGG + chr4.2:900022-900041 MS.gene62645:CDS 35.0%
! TGGTAGTGCTGTTAAATGTA+AGG + chr4.2:901158-901177 MS.gene62645:CDS 35.0%
!! TTGATATTGATTGGTGTTGG+TGG - chr4.2:899968-899987 None:intergenic 35.0%
!! TTGGTGTTGTTCTTCTACTT+TGG - chr4.2:899904-899923 None:intergenic 35.0%
!! TTGTTGCTTCAAAGAGTGTT+TGG + chr4.2:900968-900987 MS.gene62645:CDS 35.0%
!! TTTGTTGTTGATGCTGACAT+TGG - chr4.2:900211-900230 None:intergenic 35.0%
!! TTTGTTGTTGCTGAGGTTAT+TGG - chr4.2:901202-901221 None:intergenic 35.0%
AAGCTCTTCAAGAAGCTTCA+AGG + chr4.2:900308-900327 MS.gene62645:CDS 40.0%
ACAAAGAAAGCTTGCAGAAG+TGG - chr4.2:900952-900971 None:intergenic 40.0%
ACAATGCAACTTAGACACAC+AGG + chr4.2:900577-900596 MS.gene62645:CDS 40.0%
CGTTGTACAACATTTCTTGG+TGG + chr4.2:900507-900526 MS.gene62645:CDS 40.0%
CTTGATTCACACCTTTCCAT+AGG - chr4.2:900778-900797 None:intergenic 40.0%
GGATATTGAACCCTATGGAA+AGG + chr4.2:900764-900783 MS.gene62645:CDS 40.0%
GTTTACATGGAAGATATGGG+TGG + chr4.2:901069-901088 MS.gene62645:CDS 40.0%
TGTTGGAATGATCAAAGGGA+AGG - chr4.2:900099-900118 None:intergenic 40.0%
TTACATGGAAGATATGGGTG+GGG + chr4.2:901071-901090 MS.gene62645:CDS 40.0%
! ACATGATTCATGGGTTGACT+TGG + chr4.2:900812-900831 MS.gene62645:CDS 40.0%
! CATGATTCATGGGTTGACTT+GGG + chr4.2:900813-900832 MS.gene62645:CDS 40.0%
! TTGGCTTCTGTTTTCACTGT+TGG - chr4.2:899885-899904 None:intergenic 40.0%
! TTTGGAGTTGAAGCACTTGT+TGG - chr4.2:899923-899942 None:intergenic 40.0%
!! GCTCCTGGTTTTTATGTCTA+TGG + chr4.2:901138-901157 MS.gene62645:CDS 40.0%
!! TGCTATGTTGGGTTGGAATA+GGG + chr4.2:900915-900934 MS.gene62645:CDS 40.0%
!! TGCTTCAAAGAGTGTTTGGA+TGG + chr4.2:900972-900991 MS.gene62645:CDS 40.0%
!! TGTCTTGTTTGTTGTTGCTG+AGG - chr4.2:901209-901228 None:intergenic 40.0%
!! TTCAAGGTTGGTACCAAACA+TGG + chr4.2:901101-901120 MS.gene62645:CDS 40.0%
!! TTGCTATGTTGGGTTGGAAT+AGG + chr4.2:900914-900933 MS.gene62645:CDS 40.0%
!!! GGGATGAAGTTTTGAGCAAA+GGG + chr4.2:900833-900852 MS.gene62645:CDS 40.0%
!!! TGGGATGAAGTTTTGAGCAA+AGG + chr4.2:900832-900851 MS.gene62645:CDS 40.0%
GCTGTTACTGCTGTTGTTGT+TGG - chr4.2:900116-900135 None:intergenic 45.0%
GTTAGGATTATGGTTGCTCC+TGG + chr4.2:901123-901142 MS.gene62645:CDS 45.0%
! GGTTGGTACCAAACATGGTT+AGG + chr4.2:901106-901125 MS.gene62645:CDS 45.0%
TGGGGACAAGTCTTCAAGGT+TGG + chr4.2:901089-901108 MS.gene62645:CDS 50.0%
!! TGTTGGGTTGGAATAGGGCT+TGG + chr4.2:900920-900939 MS.gene62645:CDS 50.0%
TGGGTGGGGACAAGTCTTCA+AGG + chr4.2:901085-901104 MS.gene62645:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 899845 901251 899845 ID=MS.gene62645
chr4.2 mRNA 899845 901251 899845 ID=MS.gene62645.t1;Parent=MS.gene62645
chr4.2 exon 899845 901251 899845 ID=MS.gene62645.t1.exon1;Parent=MS.gene62645.t1
chr4.2 CDS 899845 901251 899845 ID=cds.MS.gene62645.t1;Parent=MS.gene62645.t1
Gene Sequence

>MS.gene62645

ATGCAGATTCAAGAGGAACATGAATTTGATGAATACACCAACAGTGAAAACAGAAGCCAAAGTAGAAGAACAACACCAACAAGTGCTTCAACTCCAAAGCACAATATCAATCATCATCATCCACCAACACCAATCAATATCAACAAAAACACAAAACCAAATCACAAGAAAAAACGTTCTGAAACTGAACTAGAAGATGGTTCTGTTTCATGCAACAAATGCAGACCACATTCTCGTGACAAAATCTTCATCCTTCCCTTTGATCATTCCAACAACAACAGCAGTAACAGCAAGTATTCATCTTTGTTGCAAAGTCCAAATGGGATTTTCAAATCCATTGTTTCAAAGCTAACAAGAAAAAGTCCAATGTCAGCATCAACAACAAACAACAATGAAGAACAATGGAAAATGGCAGTAGCAGAACTTTCTCACAAACTTGTTCATGCCACAAGAAAAAGAGATGAAGCTCTTCAAGAAGCTTCAAGGCTCATGAACTCAATGTCAGAGTTAGAGAAAAAGTTGAACAAGCTTGAAATTTATTGTCACACTTTGAAATCTGGTCTTGAAGAATGTAACAATATTAATACTGCTACTAATGTTGTTGGTTCTTCTAATGTTGTTGCTCAGAAGAATTTTCATCATCATGTTCAAGACAATGACAACGTTGTACAACATTTCTTGGTGGCTGTTTCTGAAGCAAGATCTTCAGTGAGACTTCTAAGTCGTTCTTTAACAATGCAACTTAGACACACAGGAAGCAAAGTGTATGAAAAAGTTTCTTTACTTTTACAACCTTATGATATAAAAATCTCTTTTTCAAAGAATCCAAGAAGTTTGCTTTTTTACTTGGAAGCACTTTTGAACAAGACATTTTTTGAGGATTTTGAGTCAATTGGGTTTCAAAAGAATGCTTGTAATAGGATATTGAACCCTATGGAAAGGTGTGAATCAAGTTTTGCTTCATTCAACATGATTCATGGGTTGACTTGGGATGAAGTTTTGAGCAAAGGGACAAGACATTTTAGTGAAGATTTTAGTAGATTTTGTGATAGAAAAATGAGTGAAATTGTTGCTATGTTGGGTTGGAATAGGGCTTGGTCTGAACCACTTCTGCAAGCTTTCTTTGTTGCTTCAAAGAGTGTTTGGATGGTTCATTTGTTGGCTAATTCAGTTCATCCAAGTTTGCCAATTTTTAGAGTTGATAAAGGAGTCAACTTTGATTCAGTTTACATGGAAGATATGGGTGGGGACAAGTCTTCAAGGTTGGTACCAAACATGGTTAGGATTATGGTTGCTCCTGGTTTTTATGTCTATGGTAGTGCTGTTAAATGTAAGGTTCTTTGCAGGTACTTGACCAATAACCTCAGCAACAACAAACAAGACAAAGGTTTAAACCCATCACCTTAA

Protein sequence

>MS.gene62645.t1

MQIQEEHEFDEYTNSENRSQSRRTTPTSASTPKHNINHHHPPTPININKNTKPNHKKKRSETELEDGSVSCNKCRPHSRDKIFILPFDHSNNNSSNSKYSSLLQSPNGIFKSIVSKLTRKSPMSASTTNNNEEQWKMAVAELSHKLVHATRKRDEALQEASRLMNSMSELEKKLNKLEIYCHTLKSGLEECNNINTATNVVGSSNVVAQKNFHHHVQDNDNVVQHFLVAVSEARSSVRLLSRSLTMQLRHTGSKVYEKVSLLLQPYDIKISFSKNPRSLLFYLEALLNKTFFEDFESIGFQKNACNRILNPMERCESSFASFNMIHGLTWDEVLSKGTRHFSEDFSRFCDRKMSEIVAMLGWNRAWSEPLLQAFFVASKSVWMVHLLANSVHPSLPIFRVDKGVNFDSVYMEDMGGDKSSRLVPNMVRIMVAPGFYVYGSAVKCKVLCRYLTNNLSNNKQDKGLNPSP