Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95864.t1 | XP_003631021.1 | 96.8 | 402 | 5 | 1 | 67 | 468 | 80 | 473 | 1.70E-219 | 771.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95864.t1 | Q9LXU9 | 39.4 | 350 | 199 | 6 | 104 | 450 | 219 | 558 | 9.0e-65 | 249.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95864.t1 | G7LCD0 | 96.8 | 402 | 5 | 1 | 67 | 468 | 80 | 473 | 1.2e-219 | 771.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene95864 | 0.846277 | 2.40E-59 | -1.69E-46 |
| MS.gene049151 | MS.gene95864 | 0.82695 | 2.05E-54 | -1.69E-46 |
| MS.gene049153 | MS.gene95864 | 0.842548 | 2.42E-58 | -1.69E-46 |
| MS.gene049260 | MS.gene95864 | 0.859319 | 4.49E-63 | -1.69E-46 |
| MS.gene049284 | MS.gene95864 | 0.808581 | 2.89E-50 | -1.69E-46 |
| MS.gene049297 | MS.gene95864 | 0.813987 | 1.94E-51 | -1.69E-46 |
| MS.gene049346 | MS.gene95864 | 0.842159 | 3.07E-58 | -1.69E-46 |
| MS.gene049351 | MS.gene95864 | 0.874588 | 6.01E-68 | -1.69E-46 |
| MS.gene049491 | MS.gene95864 | 0.841793 | 3.83E-58 | -1.69E-46 |
| MS.gene049599 | MS.gene95864 | -0.821731 | 3.45E-53 | -1.69E-46 |
| MS.gene049661 | MS.gene95864 | 0.860875 | 1.52E-63 | -1.69E-46 |
| MS.gene049662 | MS.gene95864 | 0.870311 | 1.61E-66 | -1.69E-46 |
| MS.gene049681 | MS.gene95864 | 0.825795 | 3.86E-54 | -1.69E-46 |
| MS.gene049758 | MS.gene95864 | 0.915411 | 6.08E-85 | -1.69E-46 |
| MS.gene049910 | MS.gene95864 | 0.83801 | 3.71E-57 | -1.69E-46 |
| MS.gene049915 | MS.gene95864 | 0.820748 | 5.82E-53 | -1.69E-46 |
| MS.gene050076 | MS.gene95864 | 0.825049 | 5.79E-54 | -1.69E-46 |
| MS.gene050099 | MS.gene95864 | 0.869826 | 2.32E-66 | -1.69E-46 |
| MS.gene050128 | MS.gene95864 | 0.872288 | 3.57E-67 | -1.69E-46 |
| MS.gene050226 | MS.gene95864 | 0.80718 | 5.73E-50 | -1.69E-46 |
| MS.gene050227 | MS.gene95864 | 0.806381 | 8.45E-50 | -1.69E-46 |
| MS.gene050231 | MS.gene95864 | 0.80113 | 1.04E-48 | -1.69E-46 |
| MS.gene050301 | MS.gene95864 | 0.908847 | 1.09E-81 | -1.69E-46 |
| MS.gene050319 | MS.gene95864 | -0.813998 | 1.93E-51 | -1.69E-46 |
| MS.gene050330 | MS.gene95864 | -0.814863 | 1.24E-51 | -1.69E-46 |
| MS.gene050397 | MS.gene95864 | 0.878699 | 2.27E-69 | -1.69E-46 |
| MS.gene050404 | MS.gene95864 | 0.826085 | 3.29E-54 | -1.69E-46 |
| MS.gene050406 | MS.gene95864 | 0.824599 | 7.40E-54 | -1.69E-46 |
| MS.gene050432 | MS.gene95864 | 0.820911 | 5.34E-53 | -1.69E-46 |
| MS.gene050464 | MS.gene95864 | 0.844712 | 6.38E-59 | -1.69E-46 |
| MS.gene050516 | MS.gene95864 | 0.805649 | 1.21E-49 | -1.69E-46 |
| MS.gene050529 | MS.gene95864 | 0.860809 | 1.59E-63 | -1.69E-46 |
| MS.gene05053 | MS.gene95864 | 0.814816 | 1.27E-51 | -1.69E-46 |
| MS.gene050558 | MS.gene95864 | 0.838356 | 3.02E-57 | -1.69E-46 |
| MS.gene050623 | MS.gene95864 | -0.806174 | 9.35E-50 | -1.69E-46 |
| MS.gene050642 | MS.gene95864 | 0.837781 | 4.24E-57 | -1.69E-46 |
| MS.gene050650 | MS.gene95864 | 0.806487 | 8.03E-50 | -1.69E-46 |
| MS.gene050670 | MS.gene95864 | 0.818569 | 1.83E-52 | -1.69E-46 |
| MS.gene050762 | MS.gene95864 | 0.801214 | 1.00E-48 | -1.69E-46 |
| MS.gene050763 | MS.gene95864 | 0.804542 | 2.05E-49 | -1.69E-46 |
| MS.gene050971 | MS.gene95864 | 0.820503 | 6.63E-53 | -1.69E-46 |
| MS.gene05101 | MS.gene95864 | 0.878159 | 3.52E-69 | -1.69E-46 |
| MS.gene05105 | MS.gene95864 | 0.817813 | 2.71E-52 | -1.69E-46 |
| MS.gene05119 | MS.gene95864 | -0.819649 | 1.04E-52 | -1.69E-46 |
| MS.gene051240 | MS.gene95864 | 0.800918 | 1.15E-48 | -1.69E-46 |
| MS.gene051255 | MS.gene95864 | 0.825171 | 5.42E-54 | -1.69E-46 |
| MS.gene051360 | MS.gene95864 | 0.801547 | 8.55E-49 | -1.69E-46 |
| MS.gene051471 | MS.gene95864 | 0.864706 | 1.00E-64 | -1.69E-46 |
| MS.gene051487 | MS.gene95864 | 0.803842 | 2.87E-49 | -1.69E-46 |
| MS.gene051582 | MS.gene95864 | 0.823171 | 1.60E-53 | -1.69E-46 |
| MS.gene051662 | MS.gene95864 | 0.826359 | 2.83E-54 | -1.69E-46 |
| MS.gene051663 | MS.gene95864 | 0.853799 | 1.88E-61 | -1.69E-46 |
| MS.gene051860 | MS.gene95864 | 0.887841 | 1.00E-72 | -1.69E-46 |
| MS.gene051877 | MS.gene95864 | 0.825634 | 4.21E-54 | -1.69E-46 |
| MS.gene051983 | MS.gene95864 | 0.83362 | 4.80E-56 | -1.69E-46 |
| MS.gene052011 | MS.gene95864 | -0.83721 | 5.94E-57 | -1.69E-46 |
| MS.gene052025 | MS.gene95864 | 0.809231 | 2.10E-50 | -1.69E-46 |
| MS.gene052077 | MS.gene95864 | 0.834689 | 2.59E-56 | -1.69E-46 |
| MS.gene052078 | MS.gene95864 | 0.880364 | 5.83E-70 | -1.69E-46 |
| MS.gene052178 | MS.gene95864 | 0.89864 | 4.35E-77 | -1.69E-46 |
| MS.gene052188 | MS.gene95864 | 0.872855 | 2.31E-67 | -1.69E-46 |
| MS.gene052207 | MS.gene95864 | 0.865794 | 4.56E-65 | -1.69E-46 |
| MS.gene052253 | MS.gene95864 | 0.874275 | 7.67E-68 | -1.69E-46 |
| MS.gene052287 | MS.gene95864 | 0.840413 | 8.82E-58 | -1.69E-46 |
| MS.gene052310 | MS.gene95864 | 0.860339 | 2.21E-63 | -1.69E-46 |
| MS.gene052326 | MS.gene95864 | 0.845862 | 3.12E-59 | -1.69E-46 |
| MS.gene052340 | MS.gene95864 | 0.888155 | 7.58E-73 | -1.69E-46 |
| MS.gene052485 | MS.gene95864 | 0.83384 | 4.23E-56 | -1.69E-46 |
| MS.gene052600 | MS.gene95864 | 0.81094 | 8.98E-51 | -1.69E-46 |
| MS.gene052917 | MS.gene95864 | -0.833769 | 4.41E-56 | -1.69E-46 |
| MS.gene053073 | MS.gene95864 | 0.812823 | 3.49E-51 | -1.69E-46 |
| MS.gene053074 | MS.gene95864 | 0.803789 | 2.95E-49 | -1.69E-46 |
| MS.gene05316 | MS.gene95864 | 0.816433 | 5.54E-52 | -1.69E-46 |
| MS.gene053209 | MS.gene95864 | 0.862238 | 5.84E-64 | -1.69E-46 |
| MS.gene053557 | MS.gene95864 | 0.875586 | 2.74E-68 | -1.69E-46 |
| MS.gene053836 | MS.gene95864 | 0.914585 | 1.61E-84 | -1.69E-46 |
| MS.gene053907 | MS.gene95864 | 0.81911 | 1.38E-52 | -1.69E-46 |
| MS.gene053928 | MS.gene95864 | 0.819601 | 1.07E-52 | -1.69E-46 |
| MS.gene053971 | MS.gene95864 | 0.807409 | 5.13E-50 | -1.69E-46 |
| MS.gene054010 | MS.gene95864 | 0.862848 | 3.79E-64 | -1.69E-46 |
| MS.gene054011 | MS.gene95864 | 0.886193 | 4.23E-72 | -1.69E-46 |
| MS.gene054012 | MS.gene95864 | 0.876947 | 9.31E-69 | -1.69E-46 |
| MS.gene054013 | MS.gene95864 | 0.873435 | 1.47E-67 | -1.69E-46 |
| MS.gene054123 | MS.gene95864 | 0.837093 | 6.37E-57 | -1.69E-46 |
| MS.gene054183 | MS.gene95864 | 0.817566 | 3.09E-52 | -1.69E-46 |
| MS.gene054184 | MS.gene95864 | 0.845124 | 4.94E-59 | -1.69E-46 |
| MS.gene054185 | MS.gene95864 | 0.845982 | 2.89E-59 | -1.69E-46 |
| MS.gene054214 | MS.gene95864 | 0.83096 | 2.19E-55 | -1.69E-46 |
| MS.gene054349 | MS.gene95864 | 0.820678 | 6.04E-53 | -1.69E-46 |
| MS.gene054376 | MS.gene95864 | 0.808826 | 2.56E-50 | -1.69E-46 |
| MS.gene054473 | MS.gene95864 | 0.87948 | 1.20E-69 | -1.69E-46 |
| MS.gene054542 | MS.gene95864 | 0.842673 | 2.24E-58 | -1.69E-46 |
| MS.gene05455 | MS.gene95864 | 0.834389 | 3.08E-56 | -1.69E-46 |
| MS.gene054618 | MS.gene95864 | 0.822891 | 1.86E-53 | -1.69E-46 |
| MS.gene054930 | MS.gene95864 | 0.815035 | 1.14E-51 | -1.69E-46 |
| MS.gene054942 | MS.gene95864 | 0.81734 | 3.47E-52 | -1.69E-46 |
| MS.gene054983 | MS.gene95864 | 0.841544 | 4.46E-58 | -1.69E-46 |
| MS.gene055116 | MS.gene95864 | 0.871175 | 8.35E-67 | -1.69E-46 |
| MS.gene055133 | MS.gene95864 | 0.817976 | 2.49E-52 | -1.69E-46 |
| MS.gene055277 | MS.gene95864 | 0.908021 | 2.69E-81 | -1.69E-46 |
| MS.gene055303 | MS.gene95864 | 0.812817 | 3.50E-51 | -1.69E-46 |
| MS.gene055390 | MS.gene95864 | 0.874353 | 7.22E-68 | -1.69E-46 |
| MS.gene055422 | MS.gene95864 | 0.90628 | 1.76E-80 | -1.69E-46 |
| MS.gene055428 | MS.gene95864 | 0.9048 | 8.40E-80 | -1.69E-46 |
| MS.gene05553 | MS.gene95864 | 0.809056 | 2.28E-50 | -1.69E-46 |
| MS.gene055604 | MS.gene95864 | 0.897328 | 1.56E-76 | -1.69E-46 |
| MS.gene055666 | MS.gene95864 | 0.802309 | 5.96E-49 | -1.69E-46 |
| MS.gene055712 | MS.gene95864 | 0.838666 | 2.51E-57 | -1.69E-46 |
| MS.gene055859 | MS.gene95864 | 0.817866 | 2.64E-52 | -1.69E-46 |
| MS.gene056069 | MS.gene95864 | -0.815949 | 7.11E-52 | -1.69E-46 |
| MS.gene056191 | MS.gene95864 | 0.882287 | 1.18E-70 | -1.69E-46 |
| MS.gene056314 | MS.gene95864 | 0.84819 | 7.18E-60 | -1.69E-46 |
| MS.gene056407 | MS.gene95864 | 0.845951 | 2.95E-59 | -1.69E-46 |
| MS.gene056410 | MS.gene95864 | 0.852245 | 5.24E-61 | -1.69E-46 |
| MS.gene056609 | MS.gene95864 | 0.81892 | 1.52E-52 | -1.69E-46 |
| MS.gene056612 | MS.gene95864 | 0.885799 | 5.95E-72 | -1.69E-46 |
| MS.gene056659 | MS.gene95864 | 0.841894 | 3.60E-58 | -1.69E-46 |
| MS.gene056772 | MS.gene95864 | 0.811562 | 6.58E-51 | -1.69E-46 |
| MS.gene056796 | MS.gene95864 | 0.822315 | 2.53E-53 | -1.69E-46 |
| MS.gene056899 | MS.gene95864 | -0.818962 | 1.49E-52 | -1.69E-46 |
| MS.gene056902 | MS.gene95864 | 0.853131 | 2.93E-61 | -1.69E-46 |
| MS.gene056931 | MS.gene95864 | 0.827946 | 1.18E-54 | -1.69E-46 |
| MS.gene056933 | MS.gene95864 | 0.83092 | 2.24E-55 | -1.69E-46 |
| MS.gene056935 | MS.gene95864 | 0.811317 | 7.44E-51 | -1.69E-46 |
| MS.gene056979 | MS.gene95864 | 0.832066 | 1.17E-55 | -1.69E-46 |
| MS.gene05702 | MS.gene95864 | 0.886866 | 2.36E-72 | -1.69E-46 |
| MS.gene057121 | MS.gene95864 | 0.879242 | 1.46E-69 | -1.69E-46 |
| MS.gene057245 | MS.gene95864 | 0.850966 | 1.21E-60 | -1.69E-46 |
| MS.gene05733 | MS.gene95864 | 0.849763 | 2.62E-60 | -1.69E-46 |
| MS.gene057439 | MS.gene95864 | 0.843919 | 1.04E-58 | -1.69E-46 |
| MS.gene057448 | MS.gene95864 | 0.84611 | 2.67E-59 | -1.69E-46 |
| MS.gene057449 | MS.gene95864 | 0.836482 | 9.12E-57 | -1.69E-46 |
| MS.gene057450 | MS.gene95864 | 0.85691 | 2.34E-62 | -1.69E-46 |
| MS.gene057469 | MS.gene95864 | 0.84386 | 1.08E-58 | -1.69E-46 |
| MS.gene057749 | MS.gene95864 | 0.829345 | 5.42E-55 | -1.69E-46 |
| MS.gene057754 | MS.gene95864 | 0.817898 | 2.60E-52 | -1.69E-46 |
| MS.gene057779 | MS.gene95864 | 0.824847 | 6.46E-54 | -1.69E-46 |
| MS.gene057782 | MS.gene95864 | 0.813007 | 3.18E-51 | -1.69E-46 |
| MS.gene057794 | MS.gene95864 | 0.817021 | 4.09E-52 | -1.69E-46 |
| MS.gene057796 | MS.gene95864 | 0.844127 | 9.16E-59 | -1.69E-46 |
| MS.gene057823 | MS.gene95864 | 0.81524 | 1.02E-51 | -1.69E-46 |
| MS.gene058002 | MS.gene95864 | 0.896958 | 2.24E-76 | -1.69E-46 |
| MS.gene058082 | MS.gene95864 | 0.851551 | 8.24E-61 | -1.69E-46 |
| MS.gene05811 | MS.gene95864 | 0.923214 | 3.56E-89 | -1.69E-46 |
| MS.gene058142 | MS.gene95864 | 0.862621 | 4.45E-64 | -1.69E-46 |
| MS.gene058233 | MS.gene95864 | 0.842333 | 2.76E-58 | -1.69E-46 |
| MS.gene058236 | MS.gene95864 | 0.811361 | 7.28E-51 | -1.69E-46 |
| MS.gene058276 | MS.gene95864 | 0.833473 | 5.23E-56 | -1.69E-46 |
| MS.gene058293 | MS.gene95864 | 0.825097 | 5.64E-54 | -1.69E-46 |
| MS.gene058365 | MS.gene95864 | 0.823779 | 1.15E-53 | -1.69E-46 |
| MS.gene05842 | MS.gene95864 | 0.822521 | 2.27E-53 | -1.69E-46 |
| MS.gene058660 | MS.gene95864 | 0.874189 | 8.20E-68 | -1.69E-46 |
| MS.gene058710 | MS.gene95864 | 0.835239 | 1.88E-56 | -1.69E-46 |
| MS.gene058749 | MS.gene95864 | 0.847388 | 1.19E-59 | -1.69E-46 |
| MS.gene058787 | MS.gene95864 | 0.831318 | 1.79E-55 | -1.69E-46 |
| MS.gene058833 | MS.gene95864 | 0.814592 | 1.42E-51 | -1.69E-46 |
| MS.gene058845 | MS.gene95864 | 0.864661 | 1.04E-64 | -1.69E-46 |
| MS.gene05886 | MS.gene95864 | 0.831916 | 1.27E-55 | -1.69E-46 |
| MS.gene058928 | MS.gene95864 | 0.832944 | 7.08E-56 | -1.69E-46 |
| MS.gene05907 | MS.gene95864 | 0.903886 | 2.18E-79 | -1.69E-46 |
| MS.gene059228 | MS.gene95864 | 0.830749 | 2.46E-55 | -1.69E-46 |
| MS.gene059285 | MS.gene95864 | 0.836092 | 1.15E-56 | -1.69E-46 |
| MS.gene059294 | MS.gene95864 | 0.889643 | 2.02E-73 | -1.69E-46 |
| MS.gene059326 | MS.gene95864 | 0.805281 | 1.44E-49 | -1.69E-46 |
| MS.gene059342 | MS.gene95864 | 0.815274 | 1.01E-51 | -1.69E-46 |
| MS.gene059420 | MS.gene95864 | 0.829819 | 4.16E-55 | -1.69E-46 |
| MS.gene059421 | MS.gene95864 | 0.812623 | 3.86E-51 | -1.69E-46 |
| MS.gene059518 | MS.gene95864 | 0.892138 | 2.09E-74 | -1.69E-46 |
| MS.gene059597 | MS.gene95864 | 0.863104 | 3.16E-64 | -1.69E-46 |
| MS.gene05959 | MS.gene95864 | 0.815745 | 7.90E-52 | -1.69E-46 |
| MS.gene059676 | MS.gene95864 | 0.838887 | 2.20E-57 | -1.69E-46 |
| MS.gene059688 | MS.gene95864 | 0.862468 | 4.96E-64 | -1.69E-46 |
| MS.gene059692 | MS.gene95864 | 0.87357 | 1.33E-67 | -1.69E-46 |
| MS.gene059695 | MS.gene95864 | 0.868242 | 7.55E-66 | -1.69E-46 |
| MS.gene059708 | MS.gene95864 | 0.827336 | 1.65E-54 | -1.69E-46 |
| MS.gene05984 | MS.gene95864 | 0.91794 | 2.87E-86 | -1.69E-46 |
| MS.gene059894 | MS.gene95864 | 0.891004 | 5.90E-74 | -1.69E-46 |
| MS.gene059899 | MS.gene95864 | 0.811951 | 5.41E-51 | -1.69E-46 |
| MS.gene059974 | MS.gene95864 | 0.874829 | 4.97E-68 | -1.69E-46 |
| MS.gene060005 | MS.gene95864 | 0.845657 | 3.54E-59 | -1.69E-46 |
| MS.gene06037 | MS.gene95864 | 0.895827 | 6.62E-76 | -1.69E-46 |
| MS.gene060403 | MS.gene95864 | 0.80028 | 1.55E-48 | -1.69E-46 |
| MS.gene060407 | MS.gene95864 | 0.823251 | 1.53E-53 | -1.69E-46 |
| MS.gene060476 | MS.gene95864 | 0.869112 | 3.95E-66 | -1.69E-46 |
| MS.gene060495 | MS.gene95864 | 0.808393 | 3.17E-50 | -1.69E-46 |
| MS.gene060559 | MS.gene95864 | 0.838798 | 2.32E-57 | -1.69E-46 |
| MS.gene06090 | MS.gene95864 | 0.811435 | 7.01E-51 | -1.69E-46 |
| MS.gene060986 | MS.gene95864 | 0.820402 | 6.99E-53 | -1.69E-46 |
| MS.gene060994 | MS.gene95864 | 0.803628 | 3.18E-49 | -1.69E-46 |
| MS.gene061049 | MS.gene95864 | 0.915774 | 3.95E-85 | -1.69E-46 |
| MS.gene061051 | MS.gene95864 | -0.83041 | 2.98E-55 | -1.69E-46 |
| MS.gene061112 | MS.gene95864 | 0.824709 | 6.97E-54 | -1.69E-46 |
| MS.gene061154 | MS.gene95864 | 0.821582 | 3.74E-53 | -1.69E-46 |
| MS.gene061159 | MS.gene95864 | 0.820124 | 8.09E-53 | -1.69E-46 |
| MS.gene061265 | MS.gene95864 | 0.829616 | 4.66E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95864.t1 | MTR_8g106210 | 95.494 | 466 | 5 | 3 | 3 | 468 | 24 | 473 | 0.0 | 911 |
| MS.gene95864.t1 | MTR_4g066010 | 34.198 | 424 | 238 | 9 | 57 | 450 | 172 | 584 | 8.74e-74 | 243 |
| MS.gene95864.t1 | MTR_2g098420 | 34.925 | 398 | 230 | 6 | 70 | 443 | 188 | 580 | 5.23e-73 | 242 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene95864.t1 | AT1G12330 | 55.580 | 457 | 147 | 11 | 23 | 449 | 42 | 472 | 6.15e-172 | 493 |
| MS.gene95864.t1 | AT5G12900 | 41.139 | 316 | 179 | 4 | 138 | 450 | 247 | 558 | 1.32e-80 | 260 |
Find 63 sgRNAs with CRISPR-Local
Find 78 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCAAACATGGTTAGGATTA+TGG | 0.210083 | 4.3:+942984 | MS.gene95864:CDS |
| TTCAGTTTACATGGAAGATA+TGG | 0.260469 | 4.3:+942936 | MS.gene95864:CDS |
| CAACGTTGTACAACATTTCT+TGG | 0.272548 | 4.3:+942375 | MS.gene95864:CDS |
| TTTGTTGTTGCTGAGGTTAT+TGG | 0.282756 | 4.3:-943070 | None:intergenic |
| TATTGTCACACTTTGAAATC+TGG | 0.312009 | 4.3:+942253 | MS.gene95864:CDS |
| TTCAAAAGAATGCTTGTAAT+AGG | 0.330527 | 4.3:+942614 | MS.gene95864:CDS |
| TGCTATGTTGGGTTGGAATA+GGG | 0.335899 | 4.3:+942786 | MS.gene95864:CDS |
| TTGCTATGTTGGGTTGGAAT+AGG | 0.340560 | 4.3:+942785 | MS.gene95864:CDS |
| GCTTCATTCAACATGATTCA+TGG | 0.354899 | 4.3:+942673 | MS.gene95864:CDS |
| TTAAATGTAAGGTTCTTTGC+AGG | 0.355993 | 4.3:+943040 | MS.gene95864:CDS |
| GCTGTTACTGCTGTTGTTGT+TGG | 0.357262 | 4.3:-941984 | None:intergenic |
| CAATGAAGAACAATGGAAAA+TGG | 0.357639 | 4.3:+942105 | MS.gene95864:CDS |
| AATACTGCTACTAATGTTGT+TGG | 0.374603 | 4.3:+942298 | MS.gene95864:CDS |
| ATGGATTTGAAAATCCCATT+TGG | 0.381416 | 4.3:-942031 | None:intergenic |
| TTGTTGCTTCAAAGAGTGTT+TGG | 0.389896 | 4.3:+942839 | MS.gene95864:CDS |
| TTGGTGTTGTTCTTCTACTT+TGG | 0.395204 | 4.3:-941772 | None:intergenic |
| TGTTGGGTTGGAATAGGGCT+TGG | 0.406333 | 4.3:+942791 | MS.gene95864:CDS |
| AAGCTCTTCAAGAAGCTTCA+AGG | 0.420955 | 4.3:+942179 | MS.gene95864:CDS |
| CAACTTTGATTCAGTTTACA+TGG | 0.421670 | 4.3:+942927 | MS.gene95864:CDS |
| GGTTGGTACCAAACATGGTT+AGG | 0.425331 | 4.3:+942977 | MS.gene95864:CDS |
| ACCATAATCCTAACCATGTT+TGG | 0.426702 | 4.3:-942985 | None:intergenic |
| ACATGATTCATGGGTTGACT+TGG | 0.437332 | 4.3:+942683 | MS.gene95864:CDS |
| TTTGGAGTTGAAGCACTTGT+TGG | 0.439726 | 4.3:-941791 | None:intergenic |
| CTTTGTTGCAAAGTCCAAAT+GGG | 0.440057 | 4.3:+942017 | MS.gene95864:CDS |
| ACTTGATGGGTTTAAGGTGA+TGG | 0.440131 | 4.3:-943111 | None:intergenic |
| TGTTTGGATGGTTCATTTGT+TGG | 0.441093 | 4.3:+942855 | MS.gene95864:CDS |
| ACAAAGAAAGCTTGCAAAAG+TGG | 0.443973 | 4.3:-942820 | None:intergenic |
| TAATAGGATATTGAACCCTA+TGG | 0.449841 | 4.3:+942630 | MS.gene95864:CDS |
| GTTAGGATTATGGTTGCTCC+TGG | 0.452810 | 4.3:+942994 | MS.gene95864:CDS |
| TCAGTTTACATGGAAGATAT+GGG | 0.453427 | 4.3:+942937 | MS.gene95864:CDS |
| CTTCATTCAACATGATTCAT+GGG | 0.453738 | 4.3:+942674 | MS.gene95864:CDS |
| TTGATATTGATTGGTGTTGG+TGG | 0.458109 | 4.3:-941836 | None:intergenic |
| TCTTTGTTGCAAAGTCCAAA+TGG | 0.458726 | 4.3:+942016 | MS.gene95864:CDS |
| TGATGATTGATATTGTGCTT+TGG | 0.465945 | 4.3:-941809 | None:intergenic |
| CTTGATGGGTTTAAGGTGAT+GGG | 0.465993 | 4.3:-943110 | None:intergenic |
| AGTGAAATTGTTGCTATGTT+GGG | 0.472103 | 4.3:+942775 | MS.gene95864:CDS |
| TTGTTGATATTGATTGGTGT+TGG | 0.475429 | 4.3:-941839 | None:intergenic |
| GAGTGAAATTGTTGCTATGT+TGG | 0.475944 | 4.3:+942774 | MS.gene95864:CDS |
| CTTGATTCACACCTTTCCAT+AGG | 0.488970 | 4.3:-942646 | None:intergenic |
| TGGTAGTGCTGTTAAATGTA+AGG | 0.493435 | 4.3:+943029 | MS.gene95864:CDS |
| TTTGTTGTTGATGCTGACAT+TGG | 0.496621 | 4.3:-942079 | None:intergenic |
| TCTTGTTAGCTTTGAAACAA+TGG | 0.500929 | 4.3:-942050 | None:intergenic |
| CGTTGTACAACATTTCTTGG+TGG | 0.514695 | 4.3:+942378 | MS.gene95864:CDS |
| TTGATTCACACCTTTCCATA+GGG | 0.520693 | 4.3:-942645 | None:intergenic |
| TTCAAGGTTGGTACCAAACA+TGG | 0.524833 | 4.3:+942972 | MS.gene95864:CDS |
| TGTCTTGTTTGTTGTTGCTG+AGG | 0.529272 | 4.3:-943077 | None:intergenic |
| TGGGTGGGGACAAGTCTTCA+AGG | 0.532792 | 4.3:+942956 | MS.gene95864:CDS |
| AAATTGTTGCTATGTTGGGT+TGG | 0.545805 | 4.3:+942779 | MS.gene95864:CDS |
| TGCTTCAAAGAGTGTTTGGA+TGG | 0.549445 | 4.3:+942843 | MS.gene95864:CDS |
| ATTAAATATGCAGATTCAAG+AGG | 0.551509 | 4.3:+941709 | None:intergenic |
| TCTGAAACTGAACTAGAAGA+TGG | 0.555922 | 4.3:+941893 | MS.gene95864:CDS |
| GTTGTTGTTGGAATGATCAA+AGG | 0.567800 | 4.3:-941972 | None:intergenic |
| CATGATTCATGGGTTGACTT+GGG | 0.593679 | 4.3:+942684 | MS.gene95864:CDS |
| GGATATTGAACCCTATGGAA+AGG | 0.598272 | 4.3:+942635 | MS.gene95864:CDS |
| TTTACATGGAAGATATGGGT+GGG | 0.604216 | 4.3:+942941 | MS.gene95864:CDS |
| TTGTTGTTGGAATGATCAAA+GGG | 0.606933 | 4.3:-941971 | None:intergenic |
| CAAACAACAATGAAGAACAA+TGG | 0.609170 | 4.3:+942098 | MS.gene95864:CDS |
| TTACATGGAAGATATGGGTG+GGG | 0.625227 | 4.3:+942942 | MS.gene95864:CDS |
| TGGGGACAAGTCTTCAAGGT+TGG | 0.629308 | 4.3:+942960 | MS.gene95864:CDS |
| GTTTACATGGAAGATATGGG+TGG | 0.633305 | 4.3:+942940 | MS.gene95864:CDS |
| ACAATGCAACTTAGACACAC+AGG | 0.633999 | 4.3:+942448 | MS.gene95864:CDS |
| TGTTGGAATGATCAAAGGGA+AGG | 0.654140 | 4.3:-941967 | None:intergenic |
| AGCAACAACAAACAAGACAA+AGG | 0.670945 | 4.3:+943081 | MS.gene95864:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAGAGATTTTTATATCATA+AGG | - | chr4.3:942511-942530 | None:intergenic | 15.0% |
| ! | AAGTAAAAAAGCAAACTTCT+TGG | - | chr4.3:942544-942563 | None:intergenic | 25.0% |
| ! | TTCAAAAGAATGCTTGTAAT+AGG | + | chr4.3:942614-942633 | MS.gene95864:CDS | 25.0% |
| !! | CCTTTATCAACTCTAAAAAT+TGG | - | chr4.3:942904-942923 | None:intergenic | 25.0% |
| !!! | AAGAAGTTTGCTTTTTTACT+TGG | + | chr4.3:942543-942562 | MS.gene95864:CDS | 25.0% |
| !!! | CCAATTTTTAGAGTTGATAA+AGG | + | chr4.3:942901-942920 | MS.gene95864:CDS | 25.0% |
| !!! | GTGTTTTTGTTGATATTGAT+TGG | - | chr4.3:941848-941867 | None:intergenic | 25.0% |
| !!! | TTTGAACAAGACATTTTTTG+AGG | + | chr4.3:942573-942592 | MS.gene95864:CDS | 25.0% |
| AATACTGCTACTAATGTTGT+TGG | + | chr4.3:942298-942317 | MS.gene95864:CDS | 30.0% | |
| AGTGAAATTGTTGCTATGTT+GGG | + | chr4.3:942775-942794 | MS.gene95864:CDS | 30.0% | |
| ATGGATTTGAAAATCCCATT+TGG | - | chr4.3:942034-942053 | None:intergenic | 30.0% | |
| CAAACAACAATGAAGAACAA+TGG | + | chr4.3:942098-942117 | MS.gene95864:CDS | 30.0% | |
| CAACTTTGATTCAGTTTACA+TGG | + | chr4.3:942927-942946 | MS.gene95864:CDS | 30.0% | |
| CAATGAAGAACAATGGAAAA+TGG | + | chr4.3:942105-942124 | MS.gene95864:CDS | 30.0% | |
| CTTCATTCAACATGATTCAT+GGG | + | chr4.3:942674-942693 | MS.gene95864:CDS | 30.0% | |
| TAATAGGATATTGAACCCTA+TGG | + | chr4.3:942630-942649 | MS.gene95864:CDS | 30.0% | |
| TCAGTTTACATGGAAGATAT+GGG | + | chr4.3:942937-942956 | MS.gene95864:CDS | 30.0% | |
| TGATGATTGATATTGTGCTT+TGG | - | chr4.3:941812-941831 | None:intergenic | 30.0% | |
| TTAAATGTAAGGTTCTTTGC+AGG | + | chr4.3:943040-943059 | MS.gene95864:CDS | 30.0% | |
| TTCAGTTTACATGGAAGATA+TGG | + | chr4.3:942936-942955 | MS.gene95864:CDS | 30.0% | |
| ! | ACTCTAAAAATTGGCAAACT+TGG | - | chr4.3:942895-942914 | None:intergenic | 30.0% |
| ! | TTGTTGATATTGATTGGTGT+TGG | - | chr4.3:941842-941861 | None:intergenic | 30.0% |
| ! | TTGTTGTTGGAATGATCAAA+GGG | - | chr4.3:941974-941993 | None:intergenic | 30.0% |
| !! | TATTGTCACACTTTGAAATC+TGG | + | chr4.3:942253-942272 | MS.gene95864:CDS | 30.0% |
| !! | TCTTGTTAGCTTTGAAACAA+TGG | - | chr4.3:942053-942072 | None:intergenic | 30.0% |
| !! | TTGAGGATTTTGAGTCAATT+GGG | + | chr4.3:942590-942609 | MS.gene95864:CDS | 30.0% |
| !! | TTTGAGGATTTTGAGTCAAT+TGG | + | chr4.3:942589-942608 | MS.gene95864:CDS | 30.0% |
| !!! | GAACGTTTTTTCTTGTGATT+TGG | - | chr4.3:941875-941894 | None:intergenic | 30.0% |
| AAATTGTTGCTATGTTGGGT+TGG | + | chr4.3:942779-942798 | MS.gene95864:CDS | 35.0% | |
| ACAAAGAAAGCTTGCAAAAG+TGG | - | chr4.3:942823-942842 | None:intergenic | 35.0% | |
| ACCAAACATGGTTAGGATTA+TGG | + | chr4.3:942984-943003 | MS.gene95864:CDS | 35.0% | |
| ACCATAATCCTAACCATGTT+TGG | - | chr4.3:942988-943007 | None:intergenic | 35.0% | |
| AGCAACAACAAACAAGACAA+AGG | + | chr4.3:943081-943100 | MS.gene95864:CDS | 35.0% | |
| CAACGTTGTACAACATTTCT+TGG | + | chr4.3:942375-942394 | MS.gene95864:CDS | 35.0% | |
| CTACCATAGACATAAAAACC+AGG | - | chr4.3:943015-943034 | None:intergenic | 35.0% | |
| CTTTGTTGCAAAGTCCAAAT+GGG | + | chr4.3:942017-942036 | MS.gene95864:CDS | 35.0% | |
| GAGTGAAATTGTTGCTATGT+TGG | + | chr4.3:942774-942793 | MS.gene95864:CDS | 35.0% | |
| GCTTCATTCAACATGATTCA+TGG | + | chr4.3:942673-942692 | MS.gene95864:CDS | 35.0% | |
| TCTTTGTTGCAAAGTCCAAA+TGG | + | chr4.3:942016-942035 | MS.gene95864:CDS | 35.0% | |
| TGTTTGGATGGTTCATTTGT+TGG | + | chr4.3:942855-942874 | MS.gene95864:CDS | 35.0% | |
| TTGATTCACACCTTTCCATA+GGG | - | chr4.3:942648-942667 | None:intergenic | 35.0% | |
| TTTACATGGAAGATATGGGT+GGG | + | chr4.3:942941-942960 | MS.gene95864:CDS | 35.0% | |
| ! | AAGATTTTGTCACGAGAATG+TGG | - | chr4.3:941944-941963 | None:intergenic | 35.0% |
| ! | AGCTTCATCTCTTTTTCTTG+TGG | - | chr4.3:942164-942183 | None:intergenic | 35.0% |
| ! | GTTGTTGTTGGAATGATCAA+AGG | - | chr4.3:941975-941994 | None:intergenic | 35.0% |
| ! | TCTGAAACTGAACTAGAAGA+TGG | + | chr4.3:941893-941912 | MS.gene95864:CDS | 35.0% |
| ! | TGGTAGTGCTGTTAAATGTA+AGG | + | chr4.3:943029-943048 | MS.gene95864:CDS | 35.0% |
| !! | TTGATATTGATTGGTGTTGG+TGG | - | chr4.3:941839-941858 | None:intergenic | 35.0% |
| !! | TTGGTGTTGTTCTTCTACTT+TGG | - | chr4.3:941775-941794 | None:intergenic | 35.0% |
| !! | TTGTTGCTTCAAAGAGTGTT+TGG | + | chr4.3:942839-942858 | MS.gene95864:CDS | 35.0% |
| !! | TTTGTTGTTGATGCTGACAT+TGG | - | chr4.3:942082-942101 | None:intergenic | 35.0% |
| !! | TTTGTTGTTGCTGAGGTTAT+TGG | - | chr4.3:943073-943092 | None:intergenic | 35.0% |
| AAGCTCTTCAAGAAGCTTCA+AGG | + | chr4.3:942179-942198 | MS.gene95864:CDS | 40.0% | |
| ACAATGCAACTTAGACACAC+AGG | + | chr4.3:942448-942467 | MS.gene95864:CDS | 40.0% | |
| CGTTGTACAACATTTCTTGG+TGG | + | chr4.3:942378-942397 | MS.gene95864:CDS | 40.0% | |
| CTTGATTCACACCTTTCCAT+AGG | - | chr4.3:942649-942668 | None:intergenic | 40.0% | |
| GGATATTGAACCCTATGGAA+AGG | + | chr4.3:942635-942654 | MS.gene95864:CDS | 40.0% | |
| GTTTACATGGAAGATATGGG+TGG | + | chr4.3:942940-942959 | MS.gene95864:CDS | 40.0% | |
| TGTTGGAATGATCAAAGGGA+AGG | - | chr4.3:941970-941989 | None:intergenic | 40.0% | |
| TTACATGGAAGATATGGGTG+GGG | + | chr4.3:942942-942961 | MS.gene95864:CDS | 40.0% | |
| ! | ACATGATTCATGGGTTGACT+TGG | + | chr4.3:942683-942702 | MS.gene95864:CDS | 40.0% |
| ! | CATGATTCATGGGTTGACTT+GGG | + | chr4.3:942684-942703 | MS.gene95864:CDS | 40.0% |
| ! | TTGGCTTCTGTTTTCACTGT+TGG | - | chr4.3:941756-941775 | None:intergenic | 40.0% |
| ! | TTTGGAGTTGAAGCACTTGT+TGG | - | chr4.3:941794-941813 | None:intergenic | 40.0% |
| !! | GCTCCTGGTTTTTATGTCTA+TGG | + | chr4.3:943009-943028 | MS.gene95864:CDS | 40.0% |
| !! | TGCTATGTTGGGTTGGAATA+GGG | + | chr4.3:942786-942805 | MS.gene95864:CDS | 40.0% |
| !! | TGCTTCAAAGAGTGTTTGGA+TGG | + | chr4.3:942843-942862 | MS.gene95864:CDS | 40.0% |
| !! | TGTCTTGTTTGTTGTTGCTG+AGG | - | chr4.3:943080-943099 | None:intergenic | 40.0% |
| !! | TTCAAGGTTGGTACCAAACA+TGG | + | chr4.3:942972-942991 | MS.gene95864:CDS | 40.0% |
| !! | TTGCTATGTTGGGTTGGAAT+AGG | + | chr4.3:942785-942804 | MS.gene95864:CDS | 40.0% |
| !!! | GGGATGAAGTTTTGAGCAAA+GGG | + | chr4.3:942704-942723 | MS.gene95864:CDS | 40.0% |
| !!! | TGGGATGAAGTTTTGAGCAA+AGG | + | chr4.3:942703-942722 | MS.gene95864:CDS | 40.0% |
| GCTGTTACTGCTGTTGTTGT+TGG | - | chr4.3:941987-942006 | None:intergenic | 45.0% | |
| GTTAGGATTATGGTTGCTCC+TGG | + | chr4.3:942994-943013 | MS.gene95864:CDS | 45.0% | |
| ! | GGTTGGTACCAAACATGGTT+AGG | + | chr4.3:942977-942996 | MS.gene95864:CDS | 45.0% |
| TGGGGACAAGTCTTCAAGGT+TGG | + | chr4.3:942960-942979 | MS.gene95864:CDS | 50.0% | |
| !! | TGTTGGGTTGGAATAGGGCT+TGG | + | chr4.3:942791-942810 | MS.gene95864:CDS | 50.0% |
| TGGGTGGGGACAAGTCTTCA+AGG | + | chr4.3:942956-942975 | MS.gene95864:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 941716 | 943122 | 941716 | ID=MS.gene95864 |
| chr4.3 | mRNA | 941716 | 943122 | 941716 | ID=MS.gene95864.t1;Parent=MS.gene95864 |
| chr4.3 | exon | 941716 | 943122 | 941716 | ID=MS.gene95864.t1.exon1;Parent=MS.gene95864.t1 |
| chr4.3 | CDS | 941716 | 943122 | 941716 | ID=cds.MS.gene95864.t1;Parent=MS.gene95864.t1 |
| Gene Sequence |
| Protein sequence |
