AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene069086


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069086.t1 MTR_5g019920 96.825 126 4 0 1 126 43 168 1.86e-83 241
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069086.t1 AT3G50685 77.600 125 27 1 2 126 35 158 3.32e-63 190

Find 38 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAATCTCAATTCCTGTTAT+AGG 0.193029 5.3:+11371696 MS.gene069086:CDS
CTCCCTGCTGATCAGTGTTT+TGG 0.233472 5.3:+11371987 MS.gene069086:CDS
GGTCTTCCTAATGGTCCTTT+TGG 0.235150 5.3:+11371864 MS.gene069086:CDS
TGTTGAAGGGTTGTCATATT+TGG 0.326036 5.3:+11371899 MS.gene069086:CDS
TTTCAACAAGGTTACATTCC+TGG 0.368209 5.3:+11371960 MS.gene069086:CDS
GGGTTATTAGGTGCTGTTGA+AGG 0.373371 5.3:+11371885 MS.gene069086:CDS
GCTATTGCTGCTATCTCTGT+TGG 0.385488 5.3:-11371666 None:intergenic
TGCTGCTATCTCTGTTGGGC+TGG 0.397920 5.3:-11371661 None:intergenic
ACTGGTTCTGGTCTTCCTAA+TGG 0.397978 5.3:+11371855 MS.gene069086:CDS
CCAGGACCAGGTGGATCAAT+TGG 0.412152 5.3:+11371762 MS.gene069086:CDS
CTCTACACTCTCAAAACAAC+TGG 0.418846 5.3:+11371726 MS.gene069086:CDS
AATAACCCAAAAGGACCATT+AGG 0.423242 5.3:-11371870 None:intergenic
TAGAGAGACCAACCTATAAC+AGG 0.435409 5.3:-11371708 None:intergenic
GGATCAATTGGTGCATTAGA+AGG 0.445690 5.3:+11371774 MS.gene069086:CDS
TATCCAAAACACTGATCAGC+AGG 0.446026 5.3:-11371990 None:intergenic
CTATTGCTGCTATCTCTGTT+GGG 0.455946 5.3:-11371665 None:intergenic
TGTAAGTTATCTTGTAGTTG+TGG 0.468651 5.3:+11371797 MS.gene069086:CDS
GTAAGTTATCTTGTAGTTGT+GGG 0.479034 5.3:+11371798 MS.gene069086:CDS
TAGTTGTGGGAATTGTTGCT+TGG 0.495156 5.3:+11371811 MS.gene069086:CDS
CCAATTGATCCACCTGGTCC+TGG 0.509784 5.3:-11371762 None:intergenic
GGTTATTAGGTGCTGTTGAA+GGG 0.510819 5.3:+11371886 MS.gene069086:CDS
TGTGGTCTTCCACCAGGACC+AGG 0.516068 5.3:+11371750 MS.gene069086:CDS
AGGGTTGTCATATTTGGCAT+TGG 0.526933 5.3:+11371905 MS.gene069086:CDS
TCTCAATTCCTGTTATAGGT+TGG 0.527179 5.3:+11371700 MS.gene069086:CDS
TGATCAGCAGGGAGAGGACC+AGG 0.529852 5.3:-11371978 None:intergenic
ACAGAGATAGCAGCAATAGC+AGG 0.544713 5.3:+11371669 MS.gene069086:CDS
ACAGCACCTAATAACCCAAA+AGG 0.547610 5.3:-11371879 None:intergenic
GAGATAGCAGCAATAGCAGG+TGG 0.565377 5.3:+11371672 MS.gene069086:CDS
AAACACTGATCAGCAGGGAG+AGG 0.597184 5.3:-11371984 None:intergenic
ACTCTCAAAACAACTGGTTG+TGG 0.598063 5.3:+11371732 MS.gene069086:CDS
ATAACAAACATGGCAAAGGA+AGG 0.600615 5.3:+11371624 None:intergenic
ACTGGTTGTGGTCTTCCACC+AGG 0.608557 5.3:+11371744 MS.gene069086:CDS
CAAGATAACAAACATGGCAA+AGG 0.614035 5.3:+11371620 None:intergenic
ATTGATCCACCTGGTCCTGG+TGG 0.622088 5.3:-11371759 None:intergenic
GGTCTTCCACCAGGACCAGG+TGG 0.646487 5.3:+11371753 MS.gene069086:CDS
AATGCACCAATTGATCCACC+TGG 0.654079 5.3:-11371768 None:intergenic
AGATAGCAGCAATAGCAGGT+GGG 0.659143 5.3:+11371673 MS.gene069086:CDS
ATCCAAAACACTGATCAGCA+GGG 0.675935 5.3:-11371989 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATATACCAAAAATAAAAC+TGG + chr5.3:11371837-11371856 MS.gene069086:CDS 15.0%
! TTAATCTCAATTCCTGTTAT+AGG + chr5.3:11371696-11371715 MS.gene069086:CDS 25.0%
GTAAGTTATCTTGTAGTTGT+GGG + chr5.3:11371798-11371817 MS.gene069086:CDS 30.0%
TGTAAGTTATCTTGTAGTTG+TGG + chr5.3:11371797-11371816 MS.gene069086:CDS 30.0%
! ACCAAAAATAAAACTGGTTC+TGG + chr5.3:11371843-11371862 MS.gene069086:CDS 30.0%
!!! ACCAGAACCAGTTTTATTTT+TGG - chr5.3:11371847-11371866 None:intergenic 30.0%
!!! GTTGCTATTGTTGTTGTTTT+TGG + chr5.3:11371927-11371946 MS.gene069086:CDS 30.0%
AATAACCCAAAAGGACCATT+AGG - chr5.3:11371873-11371892 None:intergenic 35.0%
TCTCAATTCCTGTTATAGGT+TGG + chr5.3:11371700-11371719 MS.gene069086:CDS 35.0%
TGTTGAAGGGTTGTCATATT+TGG + chr5.3:11371899-11371918 MS.gene069086:CDS 35.0%
TTTCAACAAGGTTACATTCC+TGG + chr5.3:11371960-11371979 MS.gene069086:CDS 35.0%
! GGTTTGCAGTATTTTCAACA+AGG + chr5.3:11371948-11371967 MS.gene069086:CDS 35.0%
!!! AATGGTCCTTTTGGGTTATT+AGG + chr5.3:11371873-11371892 MS.gene069086:CDS 35.0%
ACAGCACCTAATAACCCAAA+AGG - chr5.3:11371882-11371901 None:intergenic 40.0%
ACTCTCAAAACAACTGGTTG+TGG + chr5.3:11371732-11371751 MS.gene069086:CDS 40.0%
AGGGTTGTCATATTTGGCAT+TGG + chr5.3:11371905-11371924 MS.gene069086:CDS 40.0%
ATCCAAAACACTGATCAGCA+GGG - chr5.3:11371992-11372011 None:intergenic 40.0%
CTATTGCTGCTATCTCTGTT+GGG - chr5.3:11371668-11371687 None:intergenic 40.0%
CTCTACACTCTCAAAACAAC+TGG + chr5.3:11371726-11371745 MS.gene069086:CDS 40.0%
GGATCAATTGGTGCATTAGA+AGG + chr5.3:11371774-11371793 MS.gene069086:CDS 40.0%
TAGAGAGACCAACCTATAAC+AGG - chr5.3:11371711-11371730 None:intergenic 40.0%
TAGTTGTGGGAATTGTTGCT+TGG + chr5.3:11371811-11371830 MS.gene069086:CDS 40.0%
TATCCAAAACACTGATCAGC+AGG - chr5.3:11371993-11372012 None:intergenic 40.0%
! GTCTTCCTAATGGTCCTTTT+GGG + chr5.3:11371865-11371884 MS.gene069086:CDS 40.0%
!! GGTTATTAGGTGCTGTTGAA+GGG + chr5.3:11371886-11371905 MS.gene069086:CDS 40.0%
AATGCACCAATTGATCCACC+TGG - chr5.3:11371771-11371790 None:intergenic 45.0%
ACAGAGATAGCAGCAATAGC+AGG + chr5.3:11371669-11371688 MS.gene069086:CDS 45.0%
AGATAGCAGCAATAGCAGGT+GGG + chr5.3:11371673-11371692 MS.gene069086:CDS 45.0%
GCTATTGCTGCTATCTCTGT+TGG - chr5.3:11371669-11371688 None:intergenic 45.0%
! GGTCTTCCTAATGGTCCTTT+TGG + chr5.3:11371864-11371883 MS.gene069086:CDS 45.0%
!! ACTGGTTCTGGTCTTCCTAA+TGG + chr5.3:11371855-11371874 MS.gene069086:CDS 45.0%
!! GGGTTATTAGGTGCTGTTGA+AGG + chr5.3:11371885-11371904 MS.gene069086:CDS 45.0%
AAACACTGATCAGCAGGGAG+AGG - chr5.3:11371987-11372006 None:intergenic 50.0%
GAGATAGCAGCAATAGCAGG+TGG + chr5.3:11371672-11371691 MS.gene069086:CDS 50.0%
! CTCCCTGCTGATCAGTGTTT+TGG + chr5.3:11371987-11372006 MS.gene069086:CDS 50.0%
ACTGGTTGTGGTCTTCCACC+AGG + chr5.3:11371744-11371763 MS.gene069086:CDS 55.0%
ATTGATCCACCTGGTCCTGG+TGG - chr5.3:11371762-11371781 None:intergenic 55.0%
CCAATTGATCCACCTGGTCC+TGG - chr5.3:11371765-11371784 None:intergenic 55.0%
CCAGGACCAGGTGGATCAAT+TGG + chr5.3:11371762-11371781 MS.gene069086:CDS 55.0%
TGCTGCTATCTCTGTTGGGC+TGG - chr5.3:11371664-11371683 None:intergenic 55.0%
TGATCAGCAGGGAGAGGACC+AGG - chr5.3:11371981-11372000 None:intergenic 60.0%
TGTGGTCTTCCACCAGGACC+AGG + chr5.3:11371750-11371769 MS.gene069086:CDS 60.0%
GGTCTTCCACCAGGACCAGG+TGG + chr5.3:11371753-11371772 MS.gene069086:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 11371633 11372013 11371633 ID=MS.gene069086
chr5.3 mRNA 11371633 11372013 11371633 ID=MS.gene069086.t1;Parent=MS.gene069086
chr5.3 exon 11371633 11372013 11371633 ID=MS.gene069086.t1.exon1;Parent=MS.gene069086.t1
chr5.3 CDS 11371633 11372013 11371633 ID=cds.MS.gene069086.t1;Parent=MS.gene069086.t1
Gene Sequence

>MS.gene069086

ATGGCAAAGGAAGGAAGCGACACTAACACCAGCCCAACAGAGATAGCAGCAATAGCAGGTGGGTTAATCTCAATTCCTGTTATAGGTTGGTCTCTCTACACTCTCAAAACAACTGGTTGTGGTCTTCCACCAGGACCAGGTGGATCAATTGGTGCATTAGAAGGTGTAAGTTATCTTGTAGTTGTGGGAATTGTTGCTTGGTCTTTATATACCAAAAATAAAACTGGTTCTGGTCTTCCTAATGGTCCTTTTGGGTTATTAGGTGCTGTTGAAGGGTTGTCATATTTGGCATTGGTTGCTATTGTTGTTGTTTTTGGTTTGCAGTATTTTCAACAAGGTTACATTCCTGGTCCTCTCCCTGCTGATCAGTGTTTTGGATAG

Protein sequence

>MS.gene069086.t1

MAKEGSDTNTSPTEIAAIAGGLISIPVIGWSLYTLKTTGCGLPPGPGGSIGALEGVSYLVVVGIVAWSLYTKNKTGSGLPNGPFGLLGAVEGLSYLALVAIVVVFGLQYFQQGYIPGPLPADQCFG