AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene74203


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74203.t1 MTR_1g115740 95.288 191 8 1 1 190 1 191 1.92e-115 326
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74203.t1 AT3G12345 53.125 192 74 6 4 185 1 186 9.18e-49 157

Find 66 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCGAAGTCTTCTCCGGATT+CGG 0.259014 1.2:-86048105 None:intergenic
TCGGATTTCTGCTTTCTTGC+CGG 0.273376 1.2:-86048168 None:intergenic
GGTGGGTTGAAGGTGGAGTT+AGG 0.286204 1.2:+86048281 MS.gene74203:CDS
GTCTTCTCCGGATTCGGGTT+TGG 0.296851 1.2:-86048099 None:intergenic
TCGAAGTCTTCTCCGGATTC+GGG 0.304678 1.2:-86048104 None:intergenic
CCGCTGAAAGAACCGGTTTC+CGG 0.306728 1.2:+86048260 MS.gene74203:CDS
TCGGGGTCGGGACCGGGTTC+CGG 0.325614 1.2:+86048218 MS.gene74203:CDS
TTAGTTGAAGAAGAATTATC+AGG 0.339500 1.2:-86048500 None:intergenic
GAAGAAGAAGAAAGTGGAAA+TGG 0.354465 1.2:-86047993 None:intergenic
GGCTACATCGGGGTCGGGAC+CGG 0.376853 1.2:+86048211 MS.gene74203:CDS
AATGGTAGAATTGCATTACT+TGG 0.377104 1.2:+86048329 MS.gene74203:CDS
AATTGCATTACTTGGATTAA+CGG 0.390474 1.2:+86048337 MS.gene74203:CDS
TTGTTGGTGGAGCTTGCAAC+TGG 0.404741 1.2:+86048365 MS.gene74203:CDS
AAATCTAACAAGGCTACATC+GGG 0.409096 1.2:+86048200 MS.gene74203:CDS
GGAGGGTGGTGTTTGAGGTT+CGG 0.426231 1.2:-86048063 None:intergenic
GAGTCGGAGGGTGGTGTTTG+AGG 0.427392 1.2:-86048068 None:intergenic
GGGTCTCCGGAGAAAAGATT+TGG 0.433276 1.2:-86048015 None:intergenic
ACCGGTTCTTTCAGCGGCAC+CGG 0.435063 1.2:-86048254 None:intergenic
GCCGGTGCCGCTGAAAGAAC+CGG 0.443892 1.2:+86048253 MS.gene74203:CDS
ACCGGTTTCCGGTGGGTTGA+AGG 0.446296 1.2:+86048271 MS.gene74203:CDS
TCTCCGGATTCGGGTTTGGA+TGG 0.448986 1.2:-86048095 None:intergenic
ACCTTCAACCCACCGGAAAC+CGG 0.463956 1.2:-86048272 None:intergenic
GCTACATCGGGGTCGGGACC+GGG 0.464069 1.2:+86048212 MS.gene74203:CDS
TGGATTAACGGCACTGTTGT+TGG 0.466065 1.2:+86048349 MS.gene74203:CDS
AAGAAGAAGAAAGTGGAAAT+GGG 0.484348 1.2:-86047992 None:intergenic
GATCCATCCAAACCCGAATC+CGG 0.485651 1.2:+86048092 MS.gene74203:CDS
AGATACAGTGAGAGATTGAA+TGG 0.486900 1.2:+86048311 MS.gene74203:CDS
GTGGGTTGAAGGTGGAGTTA+GGG 0.495100 1.2:+86048282 MS.gene74203:CDS
AACAAGGCTACATCGGGGTC+GGG 0.495458 1.2:+86048206 MS.gene74203:CDS
AAAATCTAACAAGGCTACAT+CGG 0.499533 1.2:+86048199 MS.gene74203:CDS
TGAAGAGAAGGAAGGGTCTC+CGG 0.503944 1.2:-86048028 None:intergenic
CTGAAAGAACCGGTTTCCGG+TGG 0.509695 1.2:+86048263 MS.gene74203:CDS
TGCCGGTGCCTCTCTTGTAA+CGG 0.509867 1.2:-86048151 None:intergenic
TGCCTCTCTTGTAACGGATA+CGG 0.530086 1.2:-86048145 None:intergenic
GTGGAGCTTGCAACTGGAAA+AGG 0.541105 1.2:+86048371 MS.gene74203:CDS
ATCCGTTACAAGAGAGGCAC+CGG 0.544488 1.2:+86048149 MS.gene74203:CDS
AAGAAAGCAGAAATCCGAAA+AGG 0.544738 1.2:+86048173 MS.gene74203:CDS
AACTGAATAATCACAGTCCC+AGG 0.545871 1.2:-86048413 None:intergenic
TTTGGATGGATCCGAGTCGG+AGG 0.550758 1.2:-86048081 None:intergenic
GGAAGATACACGCCGGAACC+CGG 0.552706 1.2:-86048230 None:intergenic
TTCCGGCGTGTATCTTCCGC+CGG 0.553388 1.2:+86048235 MS.gene74203:CDS
CCGGAAACCGGTTCTTTCAG+CGG 0.554515 1.2:-86048260 None:intergenic
TAACAAGGCTACATCGGGGT+CGG 0.556442 1.2:+86048205 MS.gene74203:CDS
GCGGTCGTCGAAGTCTTCTC+CGG 0.559155 1.2:-86048111 None:intergenic
GGTTTCCGGTGGGTTGAAGG+TGG 0.563703 1.2:+86048274 MS.gene74203:CDS
TCTTGTAACGGATACGGAGT+TGG 0.566312 1.2:-86048139 None:intergenic
ATTAACGGCACTGTTGTTGG+TGG 0.571400 1.2:+86048352 MS.gene74203:CDS
GATTTGGAAGAAGAAGAAAG+TGG 0.572368 1.2:-86047999 None:intergenic
TTGGATGGATCCGAGTCGGA+GGG 0.580686 1.2:-86048080 None:intergenic
CTCCGTATCCGTTACAAGAG+AGG 0.587354 1.2:+86048143 MS.gene74203:CDS
GTTATCAATTATCACACACC+TGG 0.587535 1.2:+86048395 MS.gene74203:CDS
TGGCTCGTGTGGTGAAGAGA+AGG 0.589646 1.2:-86048040 None:intergenic
TCAAACACCACCCTCCGACT+CGG 0.590606 1.2:+86048070 MS.gene74203:CDS
GGGTGGTGTTTGAGGTTCGG+TGG 0.598214 1.2:-86048060 None:intergenic
GGTTCTTTCAGCGGCACCGG+CGG 0.605564 1.2:-86048251 None:intergenic
GATGGATCCGAGTCGGAGGG+TGG 0.610065 1.2:-86048077 None:intergenic
TGAAAGAACCGGTTTCCGGT+GGG 0.610132 1.2:+86048264 MS.gene74203:CDS
TCGTGTGGTGAAGAGAAGGA+AGG 0.614529 1.2:-86048036 None:intergenic
TTGAGGTTCGGTGGCTCGTG+TGG 0.617678 1.2:-86048051 None:intergenic
TAACTCCACCTTCAACCCAC+CGG 0.625656 1.2:-86048279 None:intergenic
CGTGTGGTGAAGAGAAGGAA+GGG 0.631699 1.2:-86048035 None:intergenic
GGGTTTGGATGGATCCGAGT+CGG 0.641564 1.2:-86048084 None:intergenic
CACCGGCGGAAGATACACGC+CGG 0.671007 1.2:-86048237 None:intergenic
TTATCAATTATCACACACCT+GGG 0.681683 1.2:+86048396 MS.gene74203:CDS
GGATACGGAGTTGGTTAATG+CGG 0.688257 1.2:-86048130 None:intergenic
AATCTAACAAGGCTACATCG+GGG 0.786766 1.2:+86048201 MS.gene74203:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GATTATTCAGTTATATTTTA+TGG + chr1.2:86048424-86048443 MS.gene74203:CDS 15.0%
! AAAAAGAGAAAATCAGTGTT+TGG + chr1.2:86048477-86048496 MS.gene74203:CDS 25.0%
! AAAAGGCAAAAAATCTAACA+AGG + chr1.2:86048190-86048209 MS.gene74203:CDS 25.0%
! AATTGCATTACTTGGATTAA+CGG + chr1.2:86048337-86048356 MS.gene74203:CDS 25.0%
! TTAGTTGAAGAAGAATTATC+AGG - chr1.2:86048503-86048522 None:intergenic 25.0%
!!! TGTTAGATTTTTTGCCTTTT+CGG - chr1.2:86048190-86048209 None:intergenic 25.0%
AAAATCTAACAAGGCTACAT+CGG + chr1.2:86048199-86048218 MS.gene74203:CDS 30.0%
AAGAAGAAGAAAGTGGAAAT+GGG - chr1.2:86047995-86048014 None:intergenic 30.0%
AATGGTAGAATTGCATTACT+TGG + chr1.2:86048329-86048348 MS.gene74203:CDS 30.0%
TTATCAATTATCACACACCT+GGG + chr1.2:86048396-86048415 MS.gene74203:CDS 30.0%
AAATCTAACAAGGCTACATC+GGG + chr1.2:86048200-86048219 MS.gene74203:CDS 35.0%
AAGAAAGCAGAAATCCGAAA+AGG + chr1.2:86048173-86048192 MS.gene74203:CDS 35.0%
AGATACAGTGAGAGATTGAA+TGG + chr1.2:86048311-86048330 MS.gene74203:CDS 35.0%
GAAGAAGAAGAAAGTGGAAA+TGG - chr1.2:86047996-86048015 None:intergenic 35.0%
GATTTGGAAGAAGAAGAAAG+TGG - chr1.2:86048002-86048021 None:intergenic 35.0%
GTTATCAATTATCACACACC+TGG + chr1.2:86048395-86048414 MS.gene74203:CDS 35.0%
! CTTCTTCCAAATCTTTTCTC+CGG + chr1.2:86048009-86048028 MS.gene74203:CDS 35.0%
AACTGAATAATCACAGTCCC+AGG - chr1.2:86048416-86048435 None:intergenic 40.0%
AATCTAACAAGGCTACATCG+GGG + chr1.2:86048201-86048220 MS.gene74203:CDS 40.0%
ATTAACGGCACTGTTGTTGG+TGG + chr1.2:86048352-86048371 MS.gene74203:CDS 45.0%
TCGGATTTCTGCTTTCTTGC+CGG - chr1.2:86048171-86048190 None:intergenic 45.0%
TGGATTAACGGCACTGTTGT+TGG + chr1.2:86048349-86048368 MS.gene74203:CDS 45.0%
! GGATACGGAGTTGGTTAATG+CGG - chr1.2:86048133-86048152 None:intergenic 45.0%
!! TCTTGTAACGGATACGGAGT+TGG - chr1.2:86048142-86048161 None:intergenic 45.0%
!! TGCCTCTCTTGTAACGGATA+CGG - chr1.2:86048148-86048167 None:intergenic 45.0%
CGTGTGGTGAAGAGAAGGAA+GGG - chr1.2:86048038-86048057 None:intergenic 50.0%
CTCCGTATCCGTTACAAGAG+AGG + chr1.2:86048143-86048162 MS.gene74203:CDS 50.0%
GATCCATCCAAACCCGAATC+CGG + chr1.2:86048092-86048111 MS.gene74203:CDS 50.0%
GGGTCTCCGGAGAAAAGATT+TGG - chr1.2:86048018-86048037 None:intergenic 50.0%
GTCGAAGTCTTCTCCGGATT+CGG - chr1.2:86048108-86048127 None:intergenic 50.0%
GTGGAGCTTGCAACTGGAAA+AGG + chr1.2:86048371-86048390 MS.gene74203:CDS 50.0%
TAACAAGGCTACATCGGGGT+CGG + chr1.2:86048205-86048224 MS.gene74203:CDS 50.0%
TAACTCCACCTTCAACCCAC+CGG - chr1.2:86048282-86048301 None:intergenic 50.0%
TCGAAGTCTTCTCCGGATTC+GGG - chr1.2:86048107-86048126 None:intergenic 50.0%
TCGTGTGGTGAAGAGAAGGA+AGG - chr1.2:86048039-86048058 None:intergenic 50.0%
TGAAGAGAAGGAAGGGTCTC+CGG - chr1.2:86048031-86048050 None:intergenic 50.0%
! ATCCGTTACAAGAGAGGCAC+CGG + chr1.2:86048149-86048168 MS.gene74203:CDS 50.0%
! GTGGGTTGAAGGTGGAGTTA+GGG + chr1.2:86048282-86048301 MS.gene74203:CDS 50.0%
! TGAAAGAACCGGTTTCCGGT+GGG + chr1.2:86048264-86048283 MS.gene74203:CDS 50.0%
! TTGTTGGTGGAGCTTGCAAC+TGG + chr1.2:86048365-86048384 MS.gene74203:CDS 50.0%
AACAAGGCTACATCGGGGTC+GGG + chr1.2:86048206-86048225 MS.gene74203:CDS 55.0%
ACCTTCAACCCACCGGAAAC+CGG - chr1.2:86048275-86048294 None:intergenic 55.0%
GGGTTTGGATGGATCCGAGT+CGG - chr1.2:86048087-86048106 None:intergenic 55.0%
TCAAACACCACCCTCCGACT+CGG + chr1.2:86048070-86048089 MS.gene74203:CDS 55.0%
TGCCGGTGCCTCTCTTGTAA+CGG - chr1.2:86048154-86048173 None:intergenic 55.0%
TGGCTCGTGTGGTGAAGAGA+AGG - chr1.2:86048043-86048062 None:intergenic 55.0%
TTGGATGGATCCGAGTCGGA+GGG - chr1.2:86048083-86048102 None:intergenic 55.0%
TTTGGATGGATCCGAGTCGG+AGG - chr1.2:86048084-86048103 None:intergenic 55.0%
! CCGCTGAAAGAACCGGTTTC+CGG + chr1.2:86048260-86048279 MS.gene74203:CDS 55.0%
! CCGGAAACCGGTTCTTTCAG+CGG - chr1.2:86048263-86048282 None:intergenic 55.0%
! CTGAAAGAACCGGTTTCCGG+TGG + chr1.2:86048263-86048282 MS.gene74203:CDS 55.0%
! GGAGGGTGGTGTTTGAGGTT+CGG - chr1.2:86048066-86048085 None:intergenic 55.0%
! GTCTTCTCCGGATTCGGGTT+TGG - chr1.2:86048102-86048121 None:intergenic 55.0%
! TCTCCGGATTCGGGTTTGGA+TGG - chr1.2:86048098-86048117 None:intergenic 55.0%
!! GGTGGGTTGAAGGTGGAGTT+AGG + chr1.2:86048281-86048300 MS.gene74203:CDS 55.0%
GAGTCGGAGGGTGGTGTTTG+AGG - chr1.2:86048071-86048090 None:intergenic 60.0%
GCGGTCGTCGAAGTCTTCTC+CGG - chr1.2:86048114-86048133 None:intergenic 60.0%
GGAAGATACACGCCGGAACC+CGG - chr1.2:86048233-86048252 None:intergenic 60.0%
TTCCGGCGTGTATCTTCCGC+CGG + chr1.2:86048235-86048254 MS.gene74203:CDS 60.0%
! ACCGGTTCTTTCAGCGGCAC+CGG - chr1.2:86048257-86048276 None:intergenic 60.0%
! GGGTGGTGTTTGAGGTTCGG+TGG - chr1.2:86048063-86048082 None:intergenic 60.0%
!! ACCGGTTTCCGGTGGGTTGA+AGG + chr1.2:86048271-86048290 MS.gene74203:CDS 60.0%
!! GGTTTCCGGTGGGTTGAAGG+TGG + chr1.2:86048274-86048293 MS.gene74203:CDS 60.0%
!! TTGAGGTTCGGTGGCTCGTG+TGG - chr1.2:86048054-86048073 None:intergenic 60.0%
CACCGGCGGAAGATACACGC+CGG - chr1.2:86048240-86048259 None:intergenic 65.0%
GATGGATCCGAGTCGGAGGG+TGG - chr1.2:86048080-86048099 None:intergenic 65.0%
! GGTTCTTTCAGCGGCACCGG+CGG - chr1.2:86048254-86048273 None:intergenic 65.0%
!! GCCGGTGCCGCTGAAAGAAC+CGG + chr1.2:86048253-86048272 MS.gene74203:CDS 65.0%
GCTACATCGGGGTCGGGACC+GGG + chr1.2:86048212-86048231 MS.gene74203:CDS 70.0%
GGCTACATCGGGGTCGGGAC+CGG + chr1.2:86048211-86048230 MS.gene74203:CDS 70.0%
TCGGGGTCGGGACCGGGTTC+CGG + chr1.2:86048218-86048237 MS.gene74203:CDS 75.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 86047954 86048526 86047954 ID=MS.gene74203
chr1.2 mRNA 86047954 86048526 86047954 ID=MS.gene74203.t1;Parent=MS.gene74203
chr1.2 exon 86047954 86048526 86047954 ID=MS.gene74203.t1.exon1;Parent=MS.gene74203.t1
chr1.2 CDS 86047954 86048526 86047954 ID=cds.MS.gene74203.t1;Parent=MS.gene74203.t1
Gene Sequence

>MS.gene74203

ATGGCGAACTTAGCAACAGCATCTGTTCAACTCTCAAACCCATTTCCACTTTCTTCTTCTTCCAAATCTTTTCTCCGGAGACCCTTCCTTCTCTTCACCACACGAGCCACCGAACCTCAAACACCACCCTCCGACTCGGATCCATCCAAACCCGAATCCGGAGAAGACTTCGACGACCGCATTAACCAACTCCGTATCCGTTACAAGAGAGGCACCGGCAAGAAAGCAGAAATCCGAAAAGGCAAAAAATCTAACAAGGCTACATCGGGGTCGGGACCGGGTTCCGGCGTGTATCTTCCGCCGGTGCCGCTGAAAGAACCGGTTTCCGGTGGGTTGAAGGTGGAGTTAGGGTTTAGCAGATACAGTGAGAGATTGAATGGTAGAATTGCATTACTTGGATTAACGGCACTGTTGTTGGTGGAGCTTGCAACTGGAAAAGGTGTTATCAATTATCACACACCTGGGACTGTGATTATTCAGTTATATTTTATGGCTGCTGTTACTGCTATTTATGTTAAGCTTGAAAAAGAGAAAATCAGTGTTTGGCCTGATAATTCTTCTTCAACTAATTAA

Protein sequence

>MS.gene74203.t1

MANLATASVQLSNPFPLSSSSKSFLRRPFLLFTTRATEPQTPPSDSDPSKPESGEDFDDRINQLRIRYKRGTGKKAEIRKGKKSNKATSGSGPGSGVYLPPVPLKEPVSGGLKVELGFSRYSERLNGRIALLGLTALLLVELATGKGVINYHTPGTVIIQLYFMAAVTAIYVKLEKEKISVWPDNSSSTN