AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene87280


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87280.t1 MTR_2g082930 97.674 215 5 0 1 215 1 215 8.29e-157 432
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene87280.t1 AT1G51100 69.780 182 47 4 37 215 35 211 1.34e-81 242

Find 38 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCAACCTATTTCAAAATT+TGG 0.094353 2.2:+14279351 None:intergenic
TTCTCTATAGGAAATGTTTC+TGG 0.167892 2.2:+14279438 None:intergenic
TCAGAAGATGTGGAATTTAA+TGG 0.265594 2.2:+14279507 None:intergenic
CTGTTCATTGTAGTGTTTGT+AGG 0.309624 2.2:+14279462 None:intergenic
GAGGTTGATGGAAATGGATT+TGG 0.350410 2.2:-14279112 MS.gene87280:CDS
GAACTTATGAAGAAGGAATA+TGG 0.383069 2.2:-14279258 MS.gene87280:CDS
ATTGTAGTGTTTGTAGGGTC+TGG 0.399199 2.2:+14279468 None:intergenic
TGTTCATTGTAGTGTTTGTA+GGG 0.442377 2.2:+14279463 None:intergenic
TTGTGTAGATGATGAGGTGC+AGG 0.469618 2.2:+14279612 None:intergenic
GTTGGAGGAACTTATGAAGA+AGG 0.476939 2.2:-14279265 MS.gene87280:CDS
TGGCTCGGCATTTGCAAGGT+CGG 0.477066 2.2:+14279374 None:intergenic
CAACCTATTTCAAAATTTGG+TGG 0.478009 2.2:+14279354 None:intergenic
AGGAAAAGTTGAGGAGAGAA+TGG 0.490855 2.2:-14279157 MS.gene87280:CDS
ATGAAGAGTTAAAATTCAAA+AGG 0.492536 2.2:+14278996 None:intergenic
GAGAGATGAAGGAAAAGTTG+AGG 0.497620 2.2:-14279166 MS.gene87280:CDS
ACAGAACTGTTGGCAAAAGT+TGG 0.501386 2.2:-14279231 MS.gene87280:CDS
TATTTCAAAATTTGGTGGCT+CGG 0.505623 2.2:+14279359 None:intergenic
TGAAAGGTGTGAAGATGTGT+TGG 0.506225 2.2:+14279578 None:intergenic
AATGGTGAAAGCTTCTGTTA+AGG 0.535346 2.2:-14279088 MS.gene87280:CDS
AGGAAGATACTTTGAATGAG+AGG 0.541636 2.2:-14279068 MS.gene87280:CDS
ATATGGTTCAACAGAACTGT+TGG 0.553725 2.2:-14279241 MS.gene87280:CDS
AAATTCTTGCTCAGAAGATG+TGG 0.558444 2.2:+14279497 None:intergenic
CAGAGATAATCAGACAAAGA+AGG 0.559049 2.2:-14279404 MS.gene87280:CDS
AGTGTTTGTAGGGTCTGGTG+TGG 0.564863 2.2:+14279473 None:intergenic
TCAGCTACCACATACCCTAC+TGG 0.567101 2.2:+14279300 None:intergenic
TGAGAAGCCAGTAGGGTATG+TGG 0.573091 2.2:-14279307 MS.gene87280:CDS
TTGGTGGCTCGGCATTTGCA+AGG 0.573166 2.2:+14279370 None:intergenic
ATCAAGCTAAGGCTAGGTGC+AGG 0.575411 2.2:-14279041 MS.gene87280:CDS
ATTAACCTTGAGAAGCCAGT+AGG 0.578990 2.2:-14279315 MS.gene87280:CDS
GTGTTTGTAGGGTCTGGTGT+GGG 0.588160 2.2:+14279474 None:intergenic
TGAGAGGCTTGATCAAGCTA+AGG 0.591500 2.2:-14279052 MS.gene87280:CDS
GATGGAAATGGATTTGGCAA+TGG 0.601943 2.2:-14279106 MS.gene87280:CDS
GGCTTGATCAAGCTAAGGCT+AGG 0.619994 2.2:-14279047 MS.gene87280:CDS
TTAACCTTGAGAAGCCAGTA+GGG 0.629422 2.2:-14279314 MS.gene87280:CDS
CATACCCTACTGGCTTCTCA+AGG 0.644603 2.2:+14279310 None:intergenic
TGTGAGGAATGTCGAAGCCA+TGG 0.662292 2.2:+14279638 None:intergenic
AAGAGATTGTGTAGATGATG+AGG 0.673918 2.2:+14279606 None:intergenic
GATGAGGTGCAGGATATGTG+AGG 0.712566 2.2:+14279622 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TTCCAACCTATTTCAAAATT+TGG + chr2.2:14279299-14279318 None:intergenic 25.0%
!!! ATTTTGAAATAGGTTGGAAA+AGG - chr2.2:14279300-14279319 MS.gene87280:CDS 25.0%
!!! TTGAACTTTTTAGAGTTTTG+AGG - chr2.2:14279516-14279535 MS.gene87280:CDS 25.0%
!!! TTTTATGAAGAAGTGCTTTT+TGG + chr2.2:14279111-14279130 None:intergenic 25.0%
CAACCTATTTCAAAATTTGG+TGG + chr2.2:14279296-14279315 None:intergenic 30.0%
GAACTTATGAAGAAGGAATA+TGG - chr2.2:14279389-14279408 MS.gene87280:CDS 30.0%
TCAGAAGATGTGGAATTTAA+TGG + chr2.2:14279143-14279162 None:intergenic 30.0%
TGTTCATTGTAGTGTTTGTA+GGG + chr2.2:14279187-14279206 None:intergenic 30.0%
TTCTCTATAGGAAATGTTTC+TGG + chr2.2:14279212-14279231 None:intergenic 30.0%
! GATCTTCTTCTTTTCTCTAT+AGG + chr2.2:14279224-14279243 None:intergenic 30.0%
! TATTTCAAAATTTGGTGGCT+CGG + chr2.2:14279291-14279310 None:intergenic 30.0%
!!! CACCAAATTTTGAAATAGGT+TGG - chr2.2:14279294-14279313 MS.gene87280:CDS 30.0%
!!! TTTTAGAGTTTTGAGGTTGA+TGG - chr2.2:14279523-14279542 MS.gene87280:CDS 30.0%
!!! TTTTGTTGTTGTGAGTTGAA+AGG + chr2.2:14279088-14279107 None:intergenic 30.0%
AAATTCTTGCTCAGAAGATG+TGG + chr2.2:14279153-14279172 None:intergenic 35.0%
AAGAGATTGTGTAGATGATG+AGG + chr2.2:14279044-14279063 None:intergenic 35.0%
AATGGTGAAAGCTTCTGTTA+AGG - chr2.2:14279559-14279578 MS.gene87280:CDS 35.0%
AGGAAGATACTTTGAATGAG+AGG - chr2.2:14279579-14279598 MS.gene87280:CDS 35.0%
ATATGGTTCAACAGAACTGT+TGG - chr2.2:14279406-14279425 MS.gene87280:CDS 35.0%
CAGAGATAATCAGACAAAGA+AGG - chr2.2:14279243-14279262 MS.gene87280:CDS 35.0%
CTGTTCATTGTAGTGTTTGT+AGG + chr2.2:14279188-14279207 None:intergenic 35.0%
!! GCAGAAATTTTGAGAGATGA+AGG - chr2.2:14279470-14279489 MS.gene87280:CDS 35.0%
!!! AGCTGATTTTTTGGAGAAGT+TGG - chr2.2:14279364-14279383 MS.gene87280:CDS 35.0%
!!! AGTTTTGAGGTTGATGGAAA+TGG - chr2.2:14279529-14279548 MS.gene87280:CDS 35.0%
!!! GAGCCACCAAATTTTGAAAT+AGG - chr2.2:14279290-14279309 MS.gene87280:CDS 35.0%
!!! GTATGTGGTAGCTGATTTTT+TGG - chr2.2:14279355-14279374 MS.gene87280:CDS 35.0%
!!! TGATTTTTTGGAGAAGTTGG+AGG - chr2.2:14279367-14279386 MS.gene87280:CDS 35.0%
ACAGAACTGTTGGCAAAAGT+TGG - chr2.2:14279416-14279435 MS.gene87280:CDS 40.0%
AGGAAAAGTTGAGGAGAGAA+TGG - chr2.2:14279490-14279509 MS.gene87280:CDS 40.0%
ATTAACCTTGAGAAGCCAGT+AGG - chr2.2:14279332-14279351 MS.gene87280:CDS 40.0%
ATTGTAGTGTTTGTAGGGTC+TGG + chr2.2:14279182-14279201 None:intergenic 40.0%
GAGAGATGAAGGAAAAGTTG+AGG - chr2.2:14279481-14279500 MS.gene87280:CDS 40.0%
GTTGGAGGAACTTATGAAGA+AGG - chr2.2:14279382-14279401 MS.gene87280:CDS 40.0%
TTAACCTTGAGAAGCCAGTA+GGG - chr2.2:14279333-14279352 MS.gene87280:CDS 40.0%
! GATGGAAATGGATTTGGCAA+TGG - chr2.2:14279541-14279560 MS.gene87280:CDS 40.0%
! TGAAAGGTGTGAAGATGTGT+TGG + chr2.2:14279072-14279091 None:intergenic 40.0%
!! GAGGTTGATGGAAATGGATT+TGG - chr2.2:14279535-14279554 MS.gene87280:CDS 40.0%
TGAGAGGCTTGATCAAGCTA+AGG - chr2.2:14279595-14279614 MS.gene87280:CDS 45.0%
TTGTGTAGATGATGAGGTGC+AGG + chr2.2:14279038-14279057 None:intergenic 45.0%
!! GCATTTGCAAGGTCGGTTTT+CGG + chr2.2:14279269-14279288 None:intergenic 45.0%
AGTGTTTGTAGGGTCTGGTG+TGG + chr2.2:14279177-14279196 None:intergenic 50.0%
ATCAAGCTAAGGCTAGGTGC+AGG - chr2.2:14279606-14279625 MS.gene87280:CDS 50.0%
GATGAGGTGCAGGATATGTG+AGG + chr2.2:14279028-14279047 None:intergenic 50.0%
GGCTTGATCAAGCTAAGGCT+AGG - chr2.2:14279600-14279619 MS.gene87280:CDS 50.0%
GTGTTTGTAGGGTCTGGTGT+GGG + chr2.2:14279176-14279195 None:intergenic 50.0%
TCAGCTACCACATACCCTAC+TGG + chr2.2:14279350-14279369 None:intergenic 50.0%
TGAGAAGCCAGTAGGGTATG+TGG - chr2.2:14279340-14279359 MS.gene87280:CDS 50.0%
!! CATACCCTACTGGCTTCTCA+AGG + chr2.2:14279340-14279359 None:intergenic 50.0%
! TGGCTCGGCATTTGCAAGGT+CGG + chr2.2:14279276-14279295 None:intergenic 55.0%
! TTGGTGGCTCGGCATTTGCA+AGG + chr2.2:14279280-14279299 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 14279011 14279658 14279011 ID=MS.gene87280
chr2.2 mRNA 14279011 14279658 14279011 ID=MS.gene87280.t1;Parent=MS.gene87280
chr2.2 exon 14279011 14279658 14279011 ID=MS.gene87280.t1.exon1;Parent=MS.gene87280.t1
chr2.2 CDS 14279011 14279658 14279011 ID=cds.MS.gene87280.t1;Parent=MS.gene87280.t1
Gene Sequence

>MS.gene87280

ATGGCTTCGACATTCCTCACATATCCTGCACCTCATCATCTACACAATCTCTTTCAAACCAACACATCTTCACACCTTTCAACTCACAACAACAAAACCAAAAAGCACTTCTTCATAAAATGCACTACTCCATTAAATTCCACATCTTCTGAGCAAGAATTTCCCACACCAGACCCTACAAACACTACAATGAACAGTCCAGAAACATTTCCTATAGAGAAAAGAAGAAGATCAGAGATAATCAGACAAAGAAGGCCGAAAACCGACCTTGCAAATGCCGAGCCACCAAATTTTGAAATAGGTTGGAAAAGGACAAAAGAGATTAACCTTGAGAAGCCAGTAGGGTATGTGGTAGCTGATTTTTTGGAGAAGTTGGAGGAACTTATGAAGAAGGAATATGGTTCAACAGAACTGTTGGCAAAAGTTGGTGAAATTGTTGCTGAAAGAGCAAGAGAAGAAGCAGAAATTTTGAGAGATGAAGGAAAAGTTGAGGAGAGAATGGTTGTTGAACTTTTTAGAGTTTTGAGGTTGATGGAAATGGATTTGGCAATGGTGAAAGCTTCTGTTAAGGAAGATACTTTGAATGAGAGGCTTGATCAAGCTAAGGCTAGGTGCAGGCAAGCTATACTTGTTTGTTATTCCTTTTGA

Protein sequence

>MS.gene87280.t1

MASTFLTYPAPHHLHNLFQTNTSSHLSTHNNKTKKHFFIKCTTPLNSTSSEQEFPTPDPTNTTMNSPETFPIEKRRRSEIIRQRRPKTDLANAEPPNFEIGWKRTKEINLEKPVGYVVADFLEKLEELMKKEYGSTELLAKVGEIVAERAREEAEILRDEGKVEERMVVELFRVLRLMEMDLAMVKASVKEDTLNERLDQAKARCRQAILVCYSF