Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004474.01.T01 | XP_013468965.1 | 99.231 | 130 | 1 | 0 | 1 | 130 | 1 | 130 | 5.73E-88 | 261 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004474.01.T01 | O22969 | 73.451 | 113 | 30 | 0 | 12 | 124 | 14 | 126 | 7.65E-55 | 171 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004474.01.T01 | A0A072VMF5 | 99.231 | 130 | 1 | 0 | 1 | 130 | 1 | 130 | 2.73e-88 | 261 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048273.01 | MsG0180004474.01 | 0.807851 | 4.130984e-50 | 2.325661e-47 |
| MsG0080048274.01 | MsG0180004474.01 | 0.800315 | 1.526580e-48 | 7.086336e-46 |
| MsG0080048929.01 | MsG0180004474.01 | 0.813454 | 2.538782e-51 | 1.658762e-48 |
| MsG0080049004.01 | MsG0180004474.01 | 0.806817 | 6.843318e-50 | 3.750689e-47 |
| MsG0080049129.01 | MsG0180004474.01 | 0.827665 | 1.380069e-54 | 1.341095e-51 |
| MsG0180000325.01 | MsG0180004474.01 | 0.822354 | 2.477845e-53 | 2.069079e-50 |
| MsG0180000638.01 | MsG0180004474.01 | 0.810518 | 1.107683e-50 | 6.691071e-48 |
| MsG0180001664.01 | MsG0180004474.01 | 0.807887 | 4.057207e-50 | 2.286307e-47 |
| MsG0180002982.01 | MsG0180004474.01 | 0.823274 | 1.513100e-53 | 1.296793e-50 |
| MsG0180003114.01 | MsG0180004474.01 | 0.804148 | 2.482953e-49 | 1.270714e-46 |
| MsG0180003155.01 | MsG0180004474.01 | 0.809466 | 1.865740e-50 | 1.096249e-47 |
| MsG0180003453.01 | MsG0180004474.01 | 0.825029 | 5.857642e-54 | 5.277735e-51 |
| MsG0180003933.01 | MsG0180004474.01 | 0.862185 | 6.061059e-64 | 1.759481e-60 |
| MsG0180004018.01 | MsG0180004474.01 | 0.805666 | 1.195771e-49 | 6.363483e-47 |
| MsG0180004458.01 | MsG0180004474.01 | 0.813783 | 2.149212e-51 | 1.416678e-48 |
| MsG0180004474.01 | MsG0180004504.01 | 0.833341 | 5.637878e-56 | 6.474714e-53 |
| MsG0180004474.01 | MsG0180004852.01 | 0.808132 | 3.599014e-50 | 2.041328e-47 |
| MsG0180004474.01 | MsG0180004865.01 | 0.825276 | 5.119931e-54 | 4.645377e-51 |
| MsG0180004474.01 | MsG0180005119.01 | 0.818470 | 1.927400e-52 | 1.444116e-49 |
| MsG0180004474.01 | MsG0180005150.01 | 0.821695 | 3.520776e-53 | 2.885233e-50 |
| MsG0180004474.01 | MsG0180005283.01 | 0.848122 | 7.495800e-60 | 1.361399e-56 |
| MsG0180004474.01 | MsG0180005416.01 | 0.800983 | 1.115586e-48 | 5.267153e-46 |
| MsG0180004474.01 | MsG0180005561.01 | 0.816551 | 5.217876e-52 | 3.708567e-49 |
| MsG0180004474.01 | MsG0180005760.01 | 0.812782 | 3.564946e-51 | 2.287160e-48 |
| MsG0180004474.01 | MsG0180005806.01 | 0.850031 | 2.208914e-60 | 4.269789e-57 |
| MsG0180004474.01 | MsG0180005879.01 | 0.831263 | 1.843425e-55 | 1.989900e-52 |
| MsG0180004474.01 | MsG0180006223.01 | 0.814071 | 1.856750e-51 | 1.233543e-48 |
| MsG0180004474.01 | MsG0280007016.01 | 0.809688 | 1.671910e-50 | 9.882022e-48 |
| MsG0180004474.01 | MsG0280007041.01 | 0.801000 | 1.106678e-48 | 5.227441e-46 |
| MsG0180004474.01 | MsG0280007418.01 | 0.813982 | 1.942798e-51 | 1.287517e-48 |
| MsG0180004474.01 | MsG0280007628.01 | 0.821412 | 4.093627e-53 | 3.327973e-50 |
| MsG0180004474.01 | MsG0280008227.01 | 0.808236 | 3.419323e-50 | 1.944830e-47 |
| MsG0180004474.01 | MsG0280008233.01 | 0.811227 | 7.777692e-51 | 4.787770e-48 |
| MsG0180004474.01 | MsG0280009434.01 | 0.842255 | 2.890672e-58 | 4.357810e-55 |
| MsG0180004474.01 | MsG0280009751.01 | 0.821807 | 3.316857e-53 | 2.726822e-50 |
| MsG0180004474.01 | MsG0280010496.01 | 0.854010 | 1.636456e-61 | 3.603813e-58 |
| MsG0180004474.01 | MsG0280011067.01 | 0.803367 | 3.606202e-49 | 1.808820e-46 |
| MsG0180004474.01 | MsG0280011189.01 | 0.814527 | 1.472451e-51 | 9.904072e-49 |
| MsG0180004474.01 | MsG0280011279.01 | 0.833019 | 6.783250e-56 | 7.714714e-53 |
| MsG0180004474.01 | MsG0380013177.01 | 0.843662 | 1.220717e-58 | 1.923549e-55 |
| MsG0180004474.01 | MsG0380013501.01 | 0.802630 | 5.120628e-49 | 2.520628e-46 |
| MsG0180004474.01 | MsG0380013530.01 | 0.817519 | 3.162026e-52 | 2.307919e-49 |
| MsG0180004474.01 | MsG0380014012.01 | 0.801913 | 7.195100e-49 | 3.477720e-46 |
| MsG0180004474.01 | MsG0380014053.01 | 0.823374 | 1.433673e-53 | 1.232100e-50 |
| MsG0180004474.01 | MsG0380014183.01 | 0.835528 | 1.592218e-56 | 1.952220e-53 |
| MsG0180004474.01 | MsG0380014465.01 | 0.814249 | 1.696069e-51 | 1.132241e-48 |
| MsG0180004474.01 | MsG0380014530.01 | 0.803919 | 2.769961e-49 | 1.409382e-46 |
| MsG0180004474.01 | MsG0380015077.01 | 0.819693 | 1.015829e-52 | 7.872766e-50 |
| MsG0180004474.01 | MsG0380015129.01 | 0.813680 | 2.264033e-51 | 1.488270e-48 |
| MsG0180004474.01 | MsG0380015501.01 | 0.804856 | 1.767058e-49 | 9.210488e-47 |
| MsG0180004474.01 | MsG0380015974.01 | 0.825164 | 5.443414e-54 | 4.923279e-51 |
| MsG0180004474.01 | MsG0380016074.01 | 0.808431 | 3.107124e-50 | 1.776328e-47 |
| MsG0180004474.01 | MsG0380016354.01 | 0.821430 | 4.055204e-53 | 3.298452e-50 |
| MsG0180004474.01 | MsG0380016452.01 | 0.829295 | 5.577104e-55 | 5.685239e-52 |
| MsG0180004474.01 | MsG0380016478.01 | 0.840634 | 7.725277e-58 | 1.107080e-54 |
| MsG0180004474.01 | MsG0380016623.01 | 0.829575 | 4.768338e-55 | 4.899650e-52 |
| MsG0180004474.01 | MsG0380017532.01 | 0.812012 | 5.250567e-51 | 3.300176e-48 |
| MsG0180004474.01 | MsG0380017647.01 | 0.825071 | 5.725211e-54 | 5.164374e-51 |
| MsG0180004474.01 | MsG0380017727.01 | 0.829810 | 4.178352e-55 | 4.323976e-52 |
| MsG0180004474.01 | MsG0380017976.01 | 0.832835 | 7.533879e-56 | 8.520794e-53 |
| MsG0180004474.01 | MsG0480018089.01 | 0.812232 | 4.702239e-51 | 2.973312e-48 |
| MsG0180004474.01 | MsG0480018108.01 | 0.829410 | 5.228855e-55 | 5.347517e-52 |
| MsG0180004474.01 | MsG0480018196.01 | 0.815979 | 7.003201e-52 | 4.900450e-49 |
| MsG0180004474.01 | MsG0480018323.01 | 0.836858 | 7.313943e-57 | 9.334469e-54 |
| MsG0180004474.01 | MsG0480018324.01 | 0.809526 | 1.811603e-50 | 1.066134e-47 |
| MsG0180004474.01 | MsG0480018328.01 | 0.801370 | 9.296924e-49 | 4.432356e-46 |
| MsG0180004474.01 | MsG0480018629.01 | 0.809987 | 1.441685e-50 | 8.588682e-48 |
| MsG0180004474.01 | MsG0480018631.01 | 0.852309 | 5.024213e-61 | 1.045606e-57 |
| MsG0180004474.01 | MsG0480018658.01 | 0.803993 | 2.673465e-49 | 1.362840e-46 |
| MsG0180004474.01 | MsG0480018844.01 | 0.865597 | 5.263494e-65 | 1.722300e-61 |
| MsG0180004474.01 | MsG0480018983.01 | 0.819839 | 9.406203e-53 | 7.320047e-50 |
| MsG0180004474.01 | MsG0480019377.01 | 0.810280 | 1.246987e-50 | 7.485103e-48 |
| MsG0180004474.01 | MsG0480019485.01 | 0.815480 | 9.048641e-52 | 6.246271e-49 |
| MsG0180004474.01 | MsG0480019693.01 | 0.807116 | 5.915450e-50 | 3.267208e-47 |
| MsG0180004474.01 | MsG0480019974.01 | 0.844789 | 6.082648e-59 | 9.929932e-56 |
| MsG0180004474.01 | MsG0480020122.01 | 0.821333 | 4.268846e-53 | 3.462874e-50 |
| MsG0180004474.01 | MsG0480021039.01 | 0.803187 | 3.929251e-49 | 1.961851e-46 |
| MsG0180004474.01 | MsG0480021307.01 | 0.802453 | 5.569066e-49 | 2.729096e-46 |
| MsG0180004474.01 | MsG0480021308.01 | 0.823479 | 1.355047e-53 | 1.168085e-50 |
| MsG0180004474.01 | MsG0480021317.01 | 0.827623 | 1.412784e-54 | 1.371066e-51 |
| MsG0180004474.01 | MsG0480021420.01 | 0.823136 | 1.629724e-53 | 1.391414e-50 |
| MsG0180004474.01 | MsG0480021654.01 | 0.846078 | 2.722978e-59 | 4.632371e-56 |
| MsG0180004474.01 | MsG0480021668.01 | 0.800050 | 1.728215e-48 | 7.968189e-46 |
| MsG0180004474.01 | MsG0480021856.01 | 0.848458 | 6.052226e-60 | 1.111478e-56 |
| MsG0180004474.01 | MsG0480021999.01 | 0.808549 | 2.932750e-50 | 1.681766e-47 |
| MsG0180004474.01 | MsG0480022804.01 | 0.812047 | 5.160775e-51 | 3.246568e-48 |
| MsG0180004474.01 | MsG0480022805.01 | 0.811138 | 8.132932e-51 | 4.994712e-48 |
| MsG0180004474.01 | MsG0480023166.01 | 0.826067 | 3.324118e-54 | 3.084608e-51 |
| MsG0180004474.01 | MsG0480023298.01 | 0.832302 | 1.021671e-55 | 1.137179e-52 |
| MsG0180004474.01 | MsG0480023371.01 | 0.837612 | 4.690560e-57 | 6.126022e-54 |
| MsG0180004474.01 | MsG0480023422.01 | 0.802774 | 4.783315e-49 | 2.363334e-46 |
| MsG0180004474.01 | MsG0480023483.01 | 0.815866 | 7.421036e-52 | 5.176710e-49 |
| MsG0180004474.01 | MsG0480023502.01 | 0.867647 | 1.172981e-65 | 4.125606e-62 |
| MsG0180004474.01 | MsG0480023505.01 | 0.808252 | 3.392571e-50 | 1.930437e-47 |
| MsG0180004474.01 | MsG0480023616.01 | 0.812561 | 3.984610e-51 | 2.541748e-48 |
| MsG0180004474.01 | MsG0480023993.01 | 0.806957 | 6.389599e-50 | 3.514729e-47 |
| MsG0180004474.01 | MsG0580024371.01 | 0.812578 | 3.951694e-51 | 2.521821e-48 |
| MsG0180004474.01 | MsG0580024386.01 | 0.802098 | 6.591432e-49 | 3.200935e-46 |
| MsG0180004474.01 | MsG0580024763.01 | 0.800377 | 1.483317e-48 | 6.896207e-46 |
| MsG0180004474.01 | MsG0580024836.01 | 0.817744 | 2.812600e-52 | 2.065625e-49 |
| MsG0180004474.01 | MsG0580024944.01 | 0.800586 | 1.344213e-48 | 6.282687e-46 |
| MsG0180004474.01 | MsG0580024982.01 | 0.829567 | 4.788682e-55 | 4.919401e-52 |
| MsG0180004474.01 | MsG0580025373.01 | 0.806524 | 7.887573e-50 | 4.290585e-47 |
| MsG0180004474.01 | MsG0580025447.01 | 0.806815 | 6.847578e-50 | 3.752876e-47 |
| MsG0180004474.01 | MsG0580025997.01 | 0.811158 | 8.052006e-51 | 4.947435e-48 |
| MsG0180004474.01 | MsG0580026211.01 | 0.809561 | 1.780601e-50 | 1.048883e-47 |
| MsG0180004474.01 | MsG0580027098.01 | 0.808198 | 3.484107e-50 | 1.979690e-47 |
| MsG0180004474.01 | MsG0580028092.01 | 0.847115 | 1.417800e-59 | 2.494357e-56 |
| MsG0180004474.01 | MsG0580028093.01 | 0.806370 | 8.501141e-50 | 4.606202e-47 |
| MsG0180004474.01 | MsG0580029719.01 | 0.816443 | 5.515096e-52 | 3.908226e-49 |
| MsG0180004474.01 | MsG0580029820.01 | 0.813505 | 2.473776e-51 | 1.618487e-48 |
| MsG0180004474.01 | MsG0580029918.01 | 0.833017 | 6.788733e-56 | 7.720614e-53 |
| MsG0180004474.01 | MsG0680030776.01 | 0.811945 | 5.429900e-51 | 3.406647e-48 |
| MsG0180004474.01 | MsG0680031791.01 | 0.814197 | 1.741296e-51 | 1.160761e-48 |
| MsG0180004474.01 | MsG0680032055.01 | 0.805628 | 1.218096e-49 | 6.475889e-47 |
| MsG0180004474.01 | MsG0680033901.01 | 0.812400 | 4.321407e-51 | 2.745002e-48 |
| MsG0180004474.01 | MsG0680033902.01 | 0.825241 | 5.218020e-54 | 4.729691e-51 |
| MsG0180004474.01 | MsG0680034431.01 | 0.805265 | 1.450842e-49 | 7.641455e-47 |
| MsG0180004474.01 | MsG0680034724.01 | 0.825228 | 5.256483e-54 | 4.762808e-51 |
| MsG0180004474.01 | MsG0680035612.01 | 0.822392 | 2.428035e-53 | 2.029749e-50 |
| MsG0180004474.01 | MsG0780037436.01 | 0.800413 | 1.458428e-48 | 6.786699e-46 |
| MsG0180004474.01 | MsG0780037655.01 | 0.810911 | 9.107234e-51 | 5.559779e-48 |
| MsG0180004474.01 | MsG0780038115.01 | 0.804793 | 1.821748e-49 | 9.479715e-47 |
| MsG0180004474.01 | MsG0780038337.01 | 0.809236 | 2.090631e-50 | 1.220922e-47 |
| MsG0180004474.01 | MsG0780038416.01 | 0.809082 | 2.255174e-50 | 1.311570e-47 |
| MsG0180004474.01 | MsG0780038848.01 | 0.823624 | 1.252829e-53 | 1.084461e-50 |
| MsG0180004474.01 | MsG0780039805.01 | 0.809734 | 1.634577e-50 | 9.673184e-48 |
| MsG0180004474.01 | MsG0780039859.01 | 0.821074 | 4.899654e-53 | 3.946326e-50 |
| MsG0180004474.01 | MsG0780040080.01 | 0.842961 | 1.877362e-58 | 2.893199e-55 |
| MsG0180004474.01 | MsG0780040319.01 | 0.822208 | 2.679271e-53 | 2.227840e-50 |
| MsG0180004474.01 | MsG0780040437.01 | 0.828873 | 7.058289e-55 | 7.104086e-52 |
| MsG0180004474.01 | MsG0780040644.01 | 0.829454 | 5.100713e-55 | 5.222832e-52 |
| MsG0180004474.01 | MsG0780041249.01 | 0.845506 | 3.891872e-59 | 6.499862e-56 |
| MsG0180004474.01 | MsG0780041289.01 | 0.814256 | 1.690481e-51 | 1.128714e-48 |
| MsG0180004474.01 | MsG0780041706.01 | 0.807204 | 5.666399e-50 | 3.136967e-47 |
| MsG0180004474.01 | MsG0880042043.01 | 0.817982 | 2.485336e-52 | 1.837083e-49 |
| MsG0180004474.01 | MsG0880042921.01 | 0.857128 | 2.016039e-62 | 4.924276e-59 |
| MsG0180004474.01 | MsG0880043032.01 | 0.820327 | 7.272206e-53 | 5.736218e-50 |
| MsG0180004474.01 | MsG0880043075.01 | 0.823314 | 1.480456e-53 | 1.270249e-50 |
| MsG0180004474.01 | MsG0880043343.01 | 0.819546 | 1.097604e-52 | 8.471899e-50 |
| MsG0180004474.01 | MsG0880043515.01 | 0.824139 | 9.487865e-54 | 8.332789e-51 |
| MsG0180004474.01 | MsG0880044352.01 | 0.802987 | 4.322571e-49 | 2.147119e-46 |
| MsG0180004474.01 | MsG0880045073.01 | 0.844671 | 6.541606e-59 | 1.063864e-55 |
| MsG0180004474.01 | MsG0880045261.01 | 0.850726 | 1.409296e-60 | 2.786529e-57 |
| MsG0180004474.01 | MsG0880045412.01 | 0.861325 | 1.110383e-63 | 3.130248e-60 |
| MsG0180004474.01 | MsG0880046135.01 | -0.805895 | 1.070193e-49 | 5.728863e-47 |
| MsG0180004474.01 | MsG0880046540.01 | 0.805191 | 1.504100e-49 | 7.906990e-47 |
| MsG0180004474.01 | MsG0880046584.01 | 0.818800 | 1.622305e-52 | 1.226520e-49 |
| MsG0180004474.01 | MsG0880046866.01 | 0.816752 | 4.702012e-52 | 3.360573e-49 |
| MsG0180004474.01 | MsG0880047540.01 | 0.816709 | 4.808312e-52 | 3.432339e-49 |
| MsG0180004474.01 | MsG0880047661.01 | 0.801280 | 9.701824e-49 | 4.614863e-46 |
| MsG0180004474.01 | MsG0880047669.01 | 0.809083 | 2.254919e-50 | 1.311442e-47 |
| MsG0180004474.01 | MsG0880047727.01 | 0.816760 | 4.682714e-52 | 3.347495e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004474.01.T01 | MTR_1g080680 | 99.231 | 130 | 1 | 0 | 1 | 130 | 1 | 130 | 6.93e-92 | 261 |
| MsG0180004474.01.T01 | MTR_2g103500 | 82.000 | 100 | 18 | 0 | 13 | 112 | 19 | 118 | 2.88e-56 | 172 |
| MsG0180004474.01.T01 | MTR_4g011000 | 75.455 | 110 | 22 | 1 | 8 | 112 | 4 | 113 | 1.51e-54 | 167 |
| MsG0180004474.01.T01 | MTR_5g006970 | 57.143 | 91 | 39 | 0 | 20 | 110 | 41 | 131 | 1.81e-29 | 104 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004474.01.T01 | AT3G04620 | 89.109 | 101 | 11 | 0 | 14 | 114 | 33 | 133 | 3.74e-63 | 190 |
| MsG0180004474.01.T01 | AT2G34160 | 73.451 | 113 | 30 | 0 | 12 | 124 | 14 | 126 | 7.80e-56 | 171 |
| MsG0180004474.01.T01 | AT1G29250 | 77.228 | 101 | 23 | 0 | 12 | 112 | 14 | 114 | 4.53e-53 | 163 |
Find 41 sgRNAs with CRISPR-Local
Find 79 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTGAATTCTGTTCTTCTTA+TGG | 0.143902 | 1:+78571383 | None:intergenic |
| AAACATCATATATTATAATA+TGG | 0.229375 | 1:-78570051 | MsG0180004474.01.T01:three_prime_UTR |
| ATTCATGGACGTAAGTAAAT+AGG | 0.243793 | 1:-78569982 | MsG0180004474.01.T01:three_prime_UTR |
| CTGAATTCTGTTCTTCTTAT+GGG | 0.253699 | 1:+78571384 | None:intergenic |
| TCAGTAGCTGCTTCTGATTC+AGG | 0.262383 | 1:+78570088 | None:intergenic |
| TCATTTGCAGTTTCAGTTAC+AGG | 0.276939 | 1:+78571409 | None:intergenic |
| CCATAAGAAGAACAGAATTC+AGG | 0.338440 | 1:-78571383 | MsG0180004474.01.T01:CDS |
| TTCATGGACGTAAGTAAATA+GGG | 0.345558 | 1:-78569981 | MsG0180004474.01.T01:three_prime_UTR |
| GTAAAAGAAAAGTGGTTTCT+TGG | 0.349846 | 1:+78571351 | None:intergenic |
| AATACAATCTAAAGTTATAA+TGG | 0.350985 | 1:+78569896 | None:intergenic |
| GAGGTTGAGCTTTCTGCTCT+TGG | 0.357617 | 1:-78570932 | MsG0180004474.01.T01:CDS |
| GAGCTTTCTGCTCTTGGAAT+GGG | 0.372225 | 1:-78570926 | MsG0180004474.01.T01:intron |
| GAAAAGTGGTTTCTTGGTGT+TGG | 0.373099 | 1:+78571357 | None:intergenic |
| AGTAAATAGGGGAGTAGAAA+AGG | 0.430445 | 1:-78569969 | MsG0180004474.01.T01:three_prime_UTR |
| TGAGCTTTCTGCTCTTGGAA+TGG | 0.445801 | 1:-78570927 | MsG0180004474.01.T01:CDS |
| AACATCATATATTATAATAT+GGG | 0.471119 | 1:-78570050 | MsG0180004474.01.T01:three_prime_UTR |
| TCCATTGTTCTTCAAAATCT+CGG | 0.472867 | 1:+78570453 | None:intergenic |
| TTTGTAGATTGAAATTGTGC+TGG | 0.477350 | 1:-78570161 | MsG0180004474.01.T01:intron |
| AATGAGGGGATCAAAGTTGT+AGG | 0.482695 | 1:-78569929 | MsG0180004474.01.T01:three_prime_UTR |
| TTCAGTTACAGGTGCAGCTA+TGG | 0.492084 | 1:+78571420 | None:intergenic |
| TCAGGTGTGGTGGTGTCAGC+AGG | 0.492551 | 1:+78570106 | None:intergenic |
| CAAAGGTCGGCTTGTGCAGA+AGG | 0.493622 | 1:-78570288 | MsG0180004474.01.T01:CDS |
| GTAAATAGGGGAGTAGAAAA+GGG | 0.509721 | 1:-78569968 | MsG0180004474.01.T01:three_prime_UTR |
| GACATCTACAGTTGGCATGA+AGG | 0.541089 | 1:-78570318 | MsG0180004474.01.T01:CDS |
| AGGGGAGTAGAAAAGGGTCG+TGG | 0.541220 | 1:-78569962 | MsG0180004474.01.T01:three_prime_UTR |
| CGTGGAGTTCAATGCAATGA+GGG | 0.553178 | 1:-78569944 | MsG0180004474.01.T01:three_prime_UTR |
| TGAGAAGTTTGACAATATGA+TGG | 0.556772 | 1:-78570131 | MsG0180004474.01.T01:CDS |
| TGCTTCTGATTCAGGTGTGG+TGG | 0.564894 | 1:+78570096 | None:intergenic |
| GAAGTATTGACATCTACAGT+TGG | 0.573871 | 1:-78570326 | MsG0180004474.01.T01:CDS |
| TTGTAGATTGAAATTGTGCT+GGG | 0.576481 | 1:-78570160 | MsG0180004474.01.T01:intron |
| AGATTGACGTAAAAGAAAAG+TGG | 0.580806 | 1:+78571343 | None:intergenic |
| GTAATCCTCTGTAACTGTAA+CGG | 0.592251 | 1:+78571456 | None:intergenic |
| AGCTGCTTCTGATTCAGGTG+TGG | 0.603045 | 1:+78570093 | None:intergenic |
| CTTTACCGTTACAGTTACAG+AGG | 0.610243 | 1:-78571461 | None:intergenic |
| TCGTGGAGTTCAATGCAATG+AGG | 0.638219 | 1:-78569945 | MsG0180004474.01.T01:three_prime_UTR |
| TGAAGGATGAGAACAAAGGT+CGG | 0.640401 | 1:-78570301 | MsG0180004474.01.T01:CDS |
| TCGGCTTGTGCAGAAGGCAA+AGG | 0.641693 | 1:-78570282 | MsG0180004474.01.T01:intron |
| TCATGGACGTAAGTAAATAG+GGG | 0.646244 | 1:-78569980 | MsG0180004474.01.T01:three_prime_UTR |
| GTGGAGTTCAATGCAATGAG+GGG | 0.682802 | 1:-78569943 | MsG0180004474.01.T01:three_prime_UTR |
| ATACATTCAGCAGCACAATG+AGG | 0.717661 | 1:-78570951 | MsG0180004474.01.T01:CDS |
| GGCATGAAGGATGAGAACAA+AGG | 0.737049 | 1:-78570305 | MsG0180004474.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAACATCATATATTATAATA+TGG | - | Chr1:78571228-78571247 | MsG0180004474.01.T01:intron | 10.0% |
| !! | AACATCATATATTATAATAT+GGG | - | Chr1:78571229-78571248 | MsG0180004474.01.T01:intron | 10.0% |
| !! | ATTTATTTACATTACTTTGA+TGG | - | Chr1:78570211-78570230 | MsG0180004474.01.T01:intron | 15.0% |
| !! | TTAAAACAATGTAATGTATA+AGG | - | Chr1:78570617-78570636 | MsG0180004474.01.T01:intron | 15.0% |
| !!! | AATACAATCTAAAGTTATAA+TGG | + | Chr1:78571386-78571405 | None:intergenic | 15.0% |
| !! | AAAAAGAAAAACAGAAAATG+AGG | - | Chr1:78570643-78570662 | MsG0180004474.01.T01:intron | 20.0% |
| !! | AATGATCATTAGATTAAGAT+CGG | - | Chr1:78570023-78570042 | MsG0180004474.01.T01:three_prime_UTR | 20.0% |
| !! | AGAAGAATCCAATTAATAAT+CGG | + | Chr1:78570433-78570452 | None:intergenic | 20.0% |
| !! | CAAATTAAAGACAAAAAGAA+GGG | + | Chr1:78570405-78570424 | None:intergenic | 20.0% |
| !! | CTAATCAATTAATGAATGTT+TGG | - | Chr1:78570922-78570941 | MsG0180004474.01.T01:intron | 20.0% |
| !! | GCTATAGAAAAGAAAAAAAA+TGG | - | Chr1:78570257-78570276 | MsG0180004474.01.T01:intron | 20.0% |
| !!! | ATTTTAATGTTAGTTGTTTC+TGG | - | Chr1:78570510-78570529 | MsG0180004474.01.T01:intron | 20.0% |
| ! | ACAAAAATGTTGACATGAAT+TGG | - | Chr1:78570774-78570793 | MsG0180004474.01.T01:intron | 25.0% |
| ! | AGAAAAGAAAAAAAATGGCT+AGG | - | Chr1:78570262-78570281 | MsG0180004474.01.T01:intron | 25.0% |
| ! | CAAAATATCAAGAAAAGTCT+AGG | + | Chr1:78571102-78571121 | None:intergenic | 25.0% |
| ! | GCAAATTAAAGACAAAAAGA+AGG | + | Chr1:78570406-78570425 | None:intergenic | 25.0% |
| ! | TAGAGTAAGAATATGAAACT+AGG | + | Chr1:78570733-78570752 | None:intergenic | 25.0% |
| ! | TATAACTCTCATTCATATGA+TGG | - | Chr1:78570889-78570908 | MsG0180004474.01.T01:intron | 25.0% |
| ! | TTCAAATTGTGATCAACTAA+TGG | - | Chr1:78570235-78570254 | MsG0180004474.01.T01:intron | 25.0% |
| !! | ACAAAAAGAAGGGAAATTTT+AGG | + | Chr1:78570395-78570414 | None:intergenic | 25.0% |
| !! | ATGTAATGTCACTTGATAAT+CGG | - | Chr1:78569969-78569988 | MsG0180004474.01.T01:three_prime_UTR | 25.0% |
| !! | GACTTTCTTAATTTTAAGCA+CGG | - | Chr1:78570045-78570064 | MsG0180004474.01.T01:three_prime_UTR | 25.0% |
| !! | TACTTTTGTTCAATTGATAG+AGG | + | Chr1:78570694-78570713 | None:intergenic | 25.0% |
| !! | TGTTGTAATTTTTGCATTCA+TGG | - | Chr1:78571282-78571301 | MsG0180004474.01.T01:intron | 25.0% |
| !!! | TCAAGGCATTATTTTTACTA+TGG | - | Chr1:78570558-78570577 | MsG0180004474.01.T01:intron | 25.0% |
| !!! | TTACATTGACTTAACTATCA+AGG | - | Chr1:78570541-78570560 | MsG0180004474.01.T01:intron | 25.0% |
| AATGATCATTGATGTAGTGA+CGG | + | Chr1:78570013-78570032 | None:intergenic | 30.0% | |
| AATTGATAGAGGATATCTGT+TGG | + | Chr1:78570683-78570702 | None:intergenic | 30.0% | |
| ACTGTTTAATCTGAATTCAG+CGG | - | Chr1:78570112-78570131 | MsG0180004474.01.T01:CDS | 30.0% | |
| AGATTGACGTAAAAGAAAAG+TGG | + | Chr1:78569939-78569958 | None:intergenic | 30.0% | |
| ATTCATGGACGTAAGTAAAT+AGG | - | Chr1:78571297-78571316 | MsG0180004474.01.T01:intron | 30.0% | |
| CTGAATTCTGTTCTTCTTAT+GGG | + | Chr1:78569898-78569917 | None:intergenic | 30.0% | |
| GTAAAAGAAAAGTGGTTTCT+TGG | + | Chr1:78569931-78569950 | None:intergenic | 30.0% | |
| TCCATTGTTCTTCAAAATCT+CGG | + | Chr1:78570829-78570848 | None:intergenic | 30.0% | |
| TGAGAAGTTTGACAATATGA+TGG | - | Chr1:78571148-78571167 | MsG0180004474.01.T01:intron | 30.0% | |
| TTACTATGGTGTGTAATGTA+AGG | - | Chr1:78570572-78570591 | MsG0180004474.01.T01:intron | 30.0% | |
| TTCATGGACGTAAGTAAATA+GGG | - | Chr1:78571298-78571317 | MsG0180004474.01.T01:intron | 30.0% | |
| TTGCACAACCGATTATTAAT+TGG | - | Chr1:78570422-78570441 | MsG0180004474.01.T01:intron | 30.0% | |
| TTGTAGATTGAAATTGTGCT+GGG | - | Chr1:78571119-78571138 | MsG0180004474.01.T01:intron | 30.0% | |
| TTTGTAGATTGAAATTGTGC+TGG | - | Chr1:78571118-78571137 | MsG0180004474.01.T01:intron | 30.0% | |
| ! | TGAATTCTCTTCATTTTCGT+CGG | + | Chr1:78571030-78571049 | None:intergenic | 30.0% |
| ! | TGTAATGTCACTTGATAATC+GGG | - | Chr1:78569970-78569989 | MsG0180004474.01.T01:three_prime_UTR | 30.0% |
| AGTAAATAGGGGAGTAGAAA+AGG | - | Chr1:78571310-78571329 | MsG0180004474.01.T01:intron | 35.0% | |
| CAAGTGACATTACATTACCT+TGG | + | Chr1:78569964-78569983 | None:intergenic | 35.0% | |
| CCATAAGAAGAACAGAATTC+AGG | - | Chr1:78569896-78569915 | MsG0180004474.01.T01:three_prime_UTR | 35.0% | |
| CCTGAATTCTGTTCTTCTTA+TGG | + | Chr1:78569899-78569918 | None:intergenic | 35.0% | |
| GTAAATAGGGGAGTAGAAAA+GGG | - | Chr1:78571311-78571330 | MsG0180004474.01.T01:intron | 35.0% | |
| GTAATCCTCTGTAACTGTAA+CGG | + | Chr1:78569826-78569845 | None:intergenic | 35.0% | |
| TATGAAACTAGGAACAGATG+AGG | + | Chr1:78570722-78570741 | None:intergenic | 35.0% | |
| TCATGGACGTAAGTAAATAG+GGG | - | Chr1:78571299-78571318 | MsG0180004474.01.T01:intron | 35.0% | |
| TCATTTGCAGTTTCAGTTAC+AGG | + | Chr1:78569873-78569892 | None:intergenic | 35.0% | |
| TGAATGTATCTCTGCATCAA+AGG | + | Chr1:78570317-78570336 | None:intergenic | 35.0% | |
| !! | GAAGTATTGACATCTACAGT+TGG | - | Chr1:78570953-78570972 | MsG0180004474.01.T01:CDS | 35.0% |
| AATGAGGGGATCAAAGTTGT+AGG | - | Chr1:78571350-78571369 | MsG0180004474.01.T01:CDS | 40.0% | |
| ACTTGCAACTGAGAAGAGTA+AGG | - | Chr1:78570848-78570867 | MsG0180004474.01.T01:intron | 40.0% | |
| ATACATTCAGCAGCACAATG+AGG | - | Chr1:78570328-78570347 | MsG0180004474.01.T01:CDS | 40.0% | |
| CATTCATGTAGTCGATGCAT+TGG | + | Chr1:78570141-78570160 | None:intergenic | 40.0% | |
| TAGTGACGGTATGAATCCAA+AGG | + | Chr1:78569999-78570018 | None:intergenic | 40.0% | |
| TGAAGGATGAGAACAAAGGT+CGG | - | Chr1:78570978-78570997 | MsG0180004474.01.T01:intron | 40.0% | |
| ! | CTTGATAATCGGGATACCTT+TGG | - | Chr1:78569980-78569999 | MsG0180004474.01.T01:three_prime_UTR | 40.0% |
| ! | GAAAAGTGGTTTCTTGGTGT+TGG | + | Chr1:78569925-78569944 | None:intergenic | 40.0% |
| !! | GCCGAGATTTTGAAGAACAA+TGG | - | Chr1:78570825-78570844 | MsG0180004474.01.T01:intron | 40.0% |
| CGTGGAGTTCAATGCAATGA+GGG | - | Chr1:78571335-78571354 | MsG0180004474.01.T01:intron | 45.0% | |
| GACATCTACAGTTGGCATGA+AGG | - | Chr1:78570961-78570980 | MsG0180004474.01.T01:intron | 45.0% | |
| GAGCTTTCTGCTCTTGGAAT+GGG | - | Chr1:78570353-78570372 | MsG0180004474.01.T01:intron | 45.0% | |
| GGCATGAAGGATGAGAACAA+AGG | - | Chr1:78570974-78570993 | MsG0180004474.01.T01:intron | 45.0% | |
| GTGGAGTTCAATGCAATGAG+GGG | - | Chr1:78571336-78571355 | MsG0180004474.01.T01:CDS | 45.0% | |
| TCAGTAGCTGCTTCTGATTC+AGG | + | Chr1:78571194-78571213 | None:intergenic | 45.0% | |
| TCGTGGAGTTCAATGCAATG+AGG | - | Chr1:78571334-78571353 | MsG0180004474.01.T01:intron | 45.0% | |
| TGAGCTTTCTGCTCTTGGAA+TGG | - | Chr1:78570352-78570371 | MsG0180004474.01.T01:intron | 45.0% | |
| TTCAGTTACAGGTGCAGCTA+TGG | + | Chr1:78569862-78569881 | None:intergenic | 45.0% | |
| ! | CTTTTACGTCAATCTCGCCA+AGG | - | Chr1:78569944-78569963 | MsG0180004474.01.T01:three_prime_UTR | 45.0% |
| AGCTGCTTCTGATTCAGGTG+TGG | + | Chr1:78571189-78571208 | None:intergenic | 50.0% | |
| GAGGTTGAGCTTTCTGCTCT+TGG | - | Chr1:78570347-78570366 | MsG0180004474.01.T01:intron | 50.0% | |
| TGCTTCTGATTCAGGTGTGG+TGG | + | Chr1:78571186-78571205 | None:intergenic | 50.0% | |
| AGGGGAGTAGAAAAGGGTCG+TGG | - | Chr1:78571317-78571336 | MsG0180004474.01.T01:intron | 55.0% | |
| ! | TCGGCTTGTGCAGAAGGCAA+AGG | - | Chr1:78570997-78571016 | MsG0180004474.01.T01:intron | 55.0% |
| !! | CAAAGGTCGGCTTGTGCAGA+AGG | - | Chr1:78570991-78571010 | MsG0180004474.01.T01:intron | 55.0% |
| ! | TCAGGTGTGGTGGTGTCAGC+AGG | + | Chr1:78571176-78571195 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 78569822 | 78571479 | 78569822 | ID=MsG0180004474.01;Name=MsG0180004474.01 |
| Chr1 | mRNA | 78569822 | 78571479 | 78569822 | ID=MsG0180004474.01.T01;Parent=MsG0180004474.01;Name=MsG0180004474.01.T01;_AED=0.47;_eAED=0.47;_QI=30|1|1|1|1|1|5|253|130 |
| Chr1 | exon | 78569822 | 78570176 | 78569822 | ID=MsG0180004474.01.T01:exon:12948;Parent=MsG0180004474.01.T01 |
| Chr1 | exon | 78570283 | 78570347 | 78570283 | ID=MsG0180004474.01.T01:exon:12947;Parent=MsG0180004474.01.T01 |
| Chr1 | exon | 78570437 | 78570499 | 78570437 | ID=MsG0180004474.01.T01:exon:12946;Parent=MsG0180004474.01.T01 |
| Chr1 | exon | 78570927 | 78570975 | 78570927 | ID=MsG0180004474.01.T01:exon:12945;Parent=MsG0180004474.01.T01 |
| Chr1 | exon | 78571336 | 78571479 | 78571336 | ID=MsG0180004474.01.T01:exon:12944;Parent=MsG0180004474.01.T01 |
| Chr1 | five_prime_UTR | 78571450 | 78571479 | 78571450 | ID=MsG0180004474.01.T01:five_prime_utr;Parent=MsG0180004474.01.T01 |
| Chr1 | CDS | 78571336 | 78571449 | 78571336 | ID=MsG0180004474.01.T01:cds;Parent=MsG0180004474.01.T01 |
| Chr1 | CDS | 78570927 | 78570975 | 78570927 | ID=MsG0180004474.01.T01:cds;Parent=MsG0180004474.01.T01 |
| Chr1 | CDS | 78570437 | 78570499 | 78570437 | ID=MsG0180004474.01.T01:cds;Parent=MsG0180004474.01.T01 |
| Chr1 | CDS | 78570283 | 78570347 | 78570283 | ID=MsG0180004474.01.T01:cds;Parent=MsG0180004474.01.T01 |
| Chr1 | CDS | 78570075 | 78570176 | 78570075 | ID=MsG0180004474.01.T01:cds;Parent=MsG0180004474.01.T01 |
| Chr1 | three_prime_UTR | 78569822 | 78570074 | 78569822 | ID=MsG0180004474.01.T01:three_prime_utr;Parent=MsG0180004474.01.T01 |
| Gene Sequence |
| Protein sequence |
