AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880045097.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045097.01.T04 MTR_2g076050 92.593 27 2 0 1 27 1 27 1.34e-12 55.1
MsG0880045097.01.T01 MTR_2g076050 94.286 35 2 0 13 47 26 60 1.34e-17 69.7
MsG0880045097.01.T02 MTR_0002s0700 95.833 72 3 0 1 72 12 83 1.36e-45 140
MsG0880045097.01.T02 MTR_4g050850 97.143 70 2 0 1 70 12 81 3.87e-44 138
MsG0880045097.01.T02 MTR_5g055290 96.923 65 2 0 1 65 12 76 3.05e-40 127
MsG0880045097.01.T02 MTR_6g007780 92.105 38 3 0 13 50 75 112 6.59e-19 74.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045097.01.T04 ATCG00570 97.436 39 1 0 1 39 1 39 4.57e-22 78.6
MsG0880045097.01.T02 ATCG00580 95.833 72 3 0 1 72 12 83 8.23e-46 141

Find 32 sgRNAs with CRISPR-Local

Find 48 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTTAGCTTACGATGTATT+TGG 0.244623 8:-54164216 MsG0880045097.01.T02:CDS
CGAATACTGGTAATAATATC+AGG 0.301237 8:+54164306 None:intergenic
TCAAAACGGCCAGTTATTAA+TGG 0.307608 8:+54164147 None:intergenic
GTAGCTCTATAATTCGGATT+AGG 0.338134 8:+54163954 None:intergenic
ATAACCAACCCGCAATGAAT+AGG 0.348030 8:+54164250 None:intergenic
TTATTACCAGTATTCGATAC+TGG 0.348418 8:-54164299 MsG0880045097.01.T02:CDS
ATACCCTCCCTATTCATTGC+GGG 0.391873 8:-54164258 MsG0880045097.01.T02:CDS
CTTTCTGTAAAATACTCGTT+TGG 0.393697 8:+54164183 None:intergenic
TAACCAACCCGCAATGAATA+GGG 0.406851 8:+54164251 None:intergenic
TGAATCGTACCAGTCTCTAC+TGG 0.407751 8:-54163897 MsG0880045097.01.T01:CDS
CAAGGAATTCCATTAATAAC+TGG 0.412082 8:-54164156 MsG0880045097.01.T02:CDS
TGTGTCGTAGCTCTATAATT+CGG 0.415726 8:+54163948 None:intergenic
AATAATCCAGTATCGAATAC+TGG 0.423687 8:+54164293 None:intergenic
TTATTAATGGAATTCCTTGT+CGG 0.434610 8:+54164160 None:intergenic
AATGGGATAGGTCCTATCTA+TGG 0.452739 8:+54164071 None:intergenic
AGGCTCTAATGACCATAGAT+AGG 0.488577 8:-54164083 None:intergenic
GTAAAATACTCGTTTGGAAG+GGG 0.495265 8:+54164189 None:intergenic
CTGTAAAATACTCGTTTGGA+AGG 0.503673 8:+54164187 None:intergenic
ATTCGGATTAGGTTTATCGT+TGG 0.512045 8:+54163965 None:intergenic
GTACGATGGTTGGCTGTTCA+CGG 0.514822 8:-54164042 MsG0880045097.01.T04:CDS
GAATCGTACCAGTCTCTACT+GGG 0.521784 8:-54163896 MsG0880045097.01.T01:CDS
GGTTGGTTATTTGTCAGCAC+GGG 0.523918 8:-54164237 MsG0880045097.01.T02:CDS
GGGTTGGTTATTTGTCAGCA+CGG 0.532372 8:-54164238 MsG0880045097.01.T02:CDS
TGTAAAATACTCGTTTGGAA+GGG 0.534074 8:+54164188 None:intergenic
CCTCCCTATTCATTGCGGGT+TGG 0.536548 8:-54164254 MsG0880045097.01.T02:CDS
GTTGTTCCAAAGAATCAAAA+CGG 0.551629 8:+54164133 None:intergenic
TAATAAACCCCAGTAGAGAC+TGG 0.572062 8:+54163888 None:intergenic
TATACCCTCCCTATTCATTG+CGG 0.586067 8:-54164259 MsG0880045097.01.T02:CDS
GTTGGCTGTTCACGGACTAG+CGG 0.657866 8:-54164034 MsG0880045097.01.T01:CDS
CAACCCGCAATGAATAGGGA+GGG 0.665638 8:+54164255 None:intergenic
CCAACCCGCAATGAATAGGG+AGG 0.678708 8:+54164254 None:intergenic
AATCGTACCAGTCTCTACTG+GGG 0.739139 8:-54163895 MsG0880045097.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATATATATATATATATT+AGG - Chr8:54164304-54164323 MsG0880045097.01.T02:CDS 0.0%
!! TATTGATCCCAAAAAAAAAA+CGG + Chr8:54164106-54164125 None:intergenic 20.0%
!!! ATTCAACATTTTGTTCATTT+GGG + Chr8:54164192-54164211 None:intergenic 20.0%
!!! TGAATTTAGTAGATCTTTTT+AGG - Chr8:54164002-54164021 MsG0880045097.01.T01:CDS 20.0%
! TTATTAATGGAATTCCTTGT+CGG + Chr8:54163951-54163970 None:intergenic 25.0%
!! CATTCAACATTTTGTTCATT+TGG + Chr8:54164193-54164212 None:intergenic 25.0%
!!! ATTTAGTAGATCTTTTTAGG+AGG - Chr8:54164005-54164024 MsG0880045097.01.T01:CDS 25.0%
AACCATCGTACTGTAAAAAT+GGG + Chr8:54164057-54164076 None:intergenic 30.0%
CAAGGAATTCCATTAATAAC+TGG - Chr8:54163952-54163971 MsG0880045097.01.T01:CDS 30.0%
CTTTCTGTAAAATACTCGTT+TGG + Chr8:54163928-54163947 None:intergenic 30.0%
GTTGTTCCAAAGAATCAAAA+CGG + Chr8:54163978-54163997 None:intergenic 30.0%
TGTAAAATACTCGTTTGGAA+GGG + Chr8:54163923-54163942 None:intergenic 30.0%
TTATTACCAGTATTCGATAC+TGG - Chr8:54163809-54163828 MsG0880045097.01.T01:CDS 30.0%
! AATAATCCAGTATCGAATAC+TGG + Chr8:54163818-54163837 None:intergenic 30.0%
CAACCATCGTACTGTAAAAA+TGG + Chr8:54164058-54164077 None:intergenic 35.0%
CTGTAAAATACTCGTTTGGA+AGG + Chr8:54163924-54163943 None:intergenic 35.0%
GATCCCAAAAAAAAAACGGT+GGG + Chr8:54164102-54164121 None:intergenic 35.0%
GTAAAATACTCGTTTGGAAG+GGG + Chr8:54163922-54163941 None:intergenic 35.0%
TCAAAACGGCCAGTTATTAA+TGG + Chr8:54163964-54163983 None:intergenic 35.0%
TCGTACTGTAAAAATGGGAT+AGG + Chr8:54164052-54164071 None:intergenic 35.0%
TGATCCCAAAAAAAAAACGG+TGG + Chr8:54164103-54164122 None:intergenic 35.0%
! ATTCGGATTAGGTTTATCGT+TGG + Chr8:54164146-54164165 None:intergenic 35.0%
! CATTTTTACAGTACGATGGT+TGG - Chr8:54164056-54164075 MsG0880045097.01.T04:CDS 35.0%
! GGTTTAGCTTACGATGTATT+TGG - Chr8:54163892-54163911 MsG0880045097.01.T01:CDS 35.0%
! GTAGCTCTATAATTCGGATT+AGG + Chr8:54164157-54164176 None:intergenic 35.0%
! TATTTTACAGAAAGCCGACA+AGG - Chr8:54163934-54163953 MsG0880045097.01.T01:CDS 35.0%
! TGTGTCGTAGCTCTATAATT+CGG + Chr8:54164163-54164182 None:intergenic 35.0%
!! ATCCCATTTTTACAGTACGA+TGG - Chr8:54164052-54164071 MsG0880045097.01.T04:CDS 35.0%
!!! GTACCCACCGTTTTTTTTTT+GGG - Chr8:54164096-54164115 None:intergenic 35.0%
AATGGGATAGGTCCTATCTA+TGG + Chr8:54164040-54164059 None:intergenic 40.0%
ATAACCAACCCGCAATGAAT+AGG + Chr8:54163861-54163880 None:intergenic 40.0%
TAACCAACCCGCAATGAATA+GGG + Chr8:54163860-54163879 None:intergenic 40.0%
TAATAAACCCCAGTAGAGAC+TGG + Chr8:54164223-54164242 None:intergenic 40.0%
TATACCCTCCCTATTCATTG+CGG - Chr8:54163849-54163868 MsG0880045097.01.T01:CDS 40.0%
! AGGCTCTAATGACCATAGAT+AGG - Chr8:54164025-54164044 MsG0880045097.01.T01:CDS 40.0%
!!! AACTGGCCGTTTTGATTCTT+TGG - Chr8:54163969-54163988 MsG0880045097.01.T01:CDS 40.0%
!!! GGTACCCACCGTTTTTTTTT+TGG - Chr8:54164095-54164114 None:intergenic 40.0%
AATCGTACCAGTCTCTACTG+GGG - Chr8:54164213-54164232 MsG0880045097.01.T02:CDS 45.0%
ATACCCTCCCTATTCATTGC+GGG - Chr8:54163850-54163869 MsG0880045097.01.T01:CDS 45.0%
GAATCGTACCAGTCTCTACT+GGG - Chr8:54164212-54164231 MsG0880045097.01.T02:CDS 45.0%
TGAATCGTACCAGTCTCTAC+TGG - Chr8:54164211-54164230 MsG0880045097.01.T02:CDS 45.0%
! GGTTGGTTATTTGTCAGCAC+GGG - Chr8:54163871-54163890 MsG0880045097.01.T01:CDS 45.0%
!! GGGTTGGTTATTTGTCAGCA+CGG - Chr8:54163870-54163889 MsG0880045097.01.T01:CDS 45.0%
CAACCCGCAATGAATAGGGA+GGG + Chr8:54163856-54163875 None:intergenic 50.0%
! GTACGATGGTTGGCTGTTCA+CGG - Chr8:54164066-54164085 MsG0880045097.01.T04:CDS 50.0%
CCAACCCGCAATGAATAGGG+AGG + Chr8:54163857-54163876 None:intergenic 55.0%
CCTCCCTATTCATTGCGGGT+TGG - Chr8:54163854-54163873 MsG0880045097.01.T01:CDS 55.0%
!! GTTGGCTGTTCACGGACTAG+CGG - Chr8:54164074-54164093 MsG0880045097.01.T04:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr8 gene 54163805 54164325 54163805 ID=MsG0880045097.01;Name=MsG0880045097.01
Chr8 mRNA 54163805 54164059 54163805 ID=MsG0880045097.01.T01;Parent=MsG0880045097.01;Name=MsG0880045097.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|0|1|0|84
Chr8 exon 54163805 54164059 54163805 ID=MsG0880045097.01.T01:exon:23391;Parent=MsG0880045097.01.T01
Chr8 CDS 54163805 54164059 54163805 ID=MsG0880045097.01.T01:cds;Parent=MsG0880045097.01.T01
Chr8 mRNA 54164107 54164325 54164107 ID=MsG0880045097.01.T02;Parent=MsG0880045097.01;Name=MsG0880045097.01.T02;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|0|1|0|72
Chr8 exon 54164107 54164325 54164107 ID=MsG0880045097.01.T02:exon:23392;Parent=MsG0880045097.01.T02
Chr8 CDS 54164107 54164325 54164107 ID=MsG0880045097.01.T02:cds;Parent=MsG0880045097.01.T02
Chr8 mRNA 54163805 54163936 54163805 ID=MsG0880045097.01.T03;Parent=MsG0880045097.01;Name=MsG0880045097.01.T03;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|0|1|0|43
Chr8 exon 54163805 54163936 54163805 ID=MsG0880045097.01.T03:exon:23393;Parent=MsG0880045097.01.T03
Chr8 CDS 54163805 54163936 54163805 ID=MsG0880045097.01.T03:cds;Parent=MsG0880045097.01.T03
Chr8 mRNA 54163978 54164097 54163978 ID=MsG0880045097.01.T04;Parent=MsG0880045097.01;Name=MsG0880045097.01.T04;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|0|1|0|39
Chr8 exon 54163978 54164097 54163978 ID=MsG0880045097.01.T04:exon:23394;Parent=MsG0880045097.01.T04
Chr8 CDS 54163978 54164097 54163978 ID=MsG0880045097.01.T04:cds;Parent=MsG0880045097.01.T04
Chr8 mRNA 54164163 54164225 54164163 ID=MsG0880045097.01.T05;Parent=MsG0880045097.01;Name=MsG0880045097.01.T05;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|20
Chr8 exon 54164163 54164225 54164163 ID=MsG0880045097.01.T05:exon:23395;Parent=MsG0880045097.01.T05
Chr8 CDS 54164163 54164225 54164163 ID=MsG0880045097.01.T05:cds;Parent=MsG0880045097.01.T05
Chr8 mRNA 54164097 54164129 54164097 ID=MsG0880045097.01.T06;Parent=MsG0880045097.01;Name=MsG0880045097.01.T06;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|10
Chr8 exon 54164097 54164129 54164097 ID=MsG0880045097.01.T06:exon:23396;Parent=MsG0880045097.01.T06
Chr8 CDS 54164097 54164129 54164097 ID=MsG0880045097.01.T06:cds;Parent=MsG0880045097.01.T06
Chr8 mRNA 54163978 54163998 54163978 ID=MsG0880045097.01.T07;Parent=MsG0880045097.01;Name=MsG0880045097.01.T07;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|6
Chr8 exon 54163978 54163998 54163978 ID=MsG0880045097.01.T07:exon:23397;Parent=MsG0880045097.01.T07
Chr8 CDS 54163978 54163998 54163978 ID=MsG0880045097.01.T07:cds;Parent=MsG0880045097.01.T07
Gene Sequence

>MsG0880045097.01.T04

ATGACCATAGATAGGACCTATCCCATTTTTACAGTACGATGGTTGGCTGTTCACGGACTAGCGGTACCCACCGTTTTTTTTTTGGGATCAATATCAGCAATGCAGTTCATCCAACGATAA

>MsG0880045097.01.T05

ATGTATTTGGAAGTCCCCTTCCAAACGAGTATTTTACAGAAAGCCGACAAGGAATTCCATTAA

>MsG0880045097.01.T01

ATGGTTGGCTGTTCACGGACTAGCGGTACCCACCGTTTTTTTTTTGGGATCAATATCAGCAATGCAGTTCATCCAACGATAAACCTAATCCGAATTATAGAGCTACGACACAATCAAACCCAAATGAACAAAATGTTGAATGAATCGTACCAGTCTCTACTGGGGTTTATTACTCATTTTTGTACTTGCTGTTTTATTTTCAAATTATTTCTTCAATTAATAGAAGAAAAGAGAATATATATATATATATATTAG

>MsG0880045097.01.T06

ATGAATTTAGTAGATCTTTTTAGGAGGCTCTAA

>MsG0880045097.01.T07

ATGCAGTTCATCCAACGATAA

>MsG0880045097.01.T02

GATATTATTACCAGTATTCGATACTGGATTATTCATAGCATTACTATACCCTCCCTATTCATTGCGGGTTGGTTATTTGTCAGCACGGGTTTAGCTTACGATGTATTTGGAAGTCCCCTTCCAAACGAGTATTTTACAGAAAGCCGACAAGGAATTCCATTAATAACTGGCCGTTTTGATTCTTTGGAACAACTCGATGAATTTAGTAGATCTTTTTAG

>MsG0880045097.01.T03

ATGAACAAAATGTTGAATGAATCGTACCAGTCTCTACTGGGGTTTATTACTCATTTTTGTACTTGCTGTTTTATTTTCAAATTATTTCTTCAATTAATAGAAGAAAAGAGAATATATATATATATATATTAG

Protein sequence

>MsG0880045097.01.T04

MTIDRTYPIFTVRWLAVHGLAVPTVFFLGSISAMQFIQR*

>MsG0880045097.01.T05

MYLEVPFQTSILQKADKEFH*

>MsG0880045097.01.T01

MVGCSRTSGTHRFFFGINISNAVHPTINLIRIIELRHNQTQMNKMLNESYQSLLGFITHFCTCCFIFKLFLQLIEEKRIYIYIY*

>MsG0880045097.01.T06

MNLVDLFRRL*

>MsG0880045097.01.T07

MQFIQR*

>MsG0880045097.01.T02

DIITSIRYWIIHSITIPSLFIAGWLFVSTGLAYDVFGSPLPNEYFTESRQGIPLITGRFDSLEQLDEFSRSF*

>MsG0880045097.01.T03

MNKMLNESYQSLLGFITHFCTCCFIFKLFLQLIEEKRIYIYIY*